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Department of Food Science and Nutrition, College of Home Economics, University of the Philippines Diliman,
Quezon City, Philippines
2
Department of Food Science and Nutrition, College of Home Economics, University of the Philippines Diliman,
Quezon City, Philippines
ABSTRACT:
This experiment aimed to understand the underlying principles behind the extraction and characterization of DNA
sample from duck egg embryo. Using the DNAs basic unit, nucleic acid, the purity and concentration was assessed
through UV-Vis Spectrophotometry at wavelengths 260 nm and 280 nm. For further analysis if the DNA sample,
agarose gel electrophoresis was employed. This technique separates DNA molecules based on its size and shape.
DNA was successfully extracted from the fertilized duck embryo which was calculated to have a low yield of 6.6% (w/v),
an estimated concentration of 23.05023334 g/mL and 10% purity.
INTRODUCTION
DNA carries genetic instructions essential in growth,
development and functioning for all known living
organisms. DNA is responsible for coding most of the
genetic information in an organism and is expressed in
an organisms physical appearance, personality, and
behavior. In 1969, the first isolation of DNA was done
by Friedrich Miescher. Combinations of mechanical
and chemical procedures are done to extract DNA from
a sample.
In this experiment, the characterization technique used
to analyze the DNA sample from the fertilized duck egg
was UV spectroscopy and agarose gel electrophoresis.
Double beam UV-Vis spectrophotometry can be used
to analyze DNA and its concentration and purity. The
aromatic nucleotide bases found in DNA absorb UV
light that allows detection of the presence DNA.
Besides UV light absorption, further analysis of the
purity of the DNA extract was done using
electrophoretic methods. Electrophoresis is an
experimental method based on the differential
movement of charged molecules in an electric field. It
can also be used to purify and analyze many other
biomolecules. The movement of molecules takes place
in a polymerized gel matrix, which was utilized because
it is more stable as compared to a free solution.
Application of electric current administers migration of
the different molecules across the rigid gel matrix. The
difference in movement of molecules was influenced by
molecule size, shape, charge, and chemical
composition.
REFERENCES
Agarose Gel Electrophoresis of DNA.. (2000).
Retrieved
from:
http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotec
h/gels/agardna.html
Burden,
D.W.
and
Whitney,
D.B.
(1995).
Biotechnology: Proteins to PCR (A Course in
Strategies and Lab Techniques). USA: Birkhauser
Boston
APPENDIX
Weight sample: 0.66 g
___0.66 g balut DNA___ x 100 = 6.6% w/v
10 mL Tris-EDTA buffer
Raw Data for UV-Vis Spectrophotometry
3
3.91837
4.42574
4.51474
4.2403
3.79224
Value
A
B
C
D
E
3
2.70785
3.88162
4.0212
3.75354
3.26792
1
2.73064
3.84675
4.10046
3.74077
3.27303
2
2.70431
3.8682
4.21225
3.74598
3.24616
dsDNA concentration=
50ug
1
x A 260 x df =50 x 4.610046667 x =23.05023334 ug / mL
mL
10
A 260 4.610046667
=
=1.1213 %Nucleic acid=10
A 280 4.111303333