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Amalina Syahirah
151141187
Objectives
1) To familiarize the students with essential theory concerning gas chromatography.
2) To be able to prepare standard limonene at different concentrations and establish
standard curve.
3) To quantify the concentration of limonene in the citrus peel extract by using gas
chromatography.
Experimental procedure
PREPARATION OF THE STANDARD SOLUTION
1.
2.
3.
4.
5.
Prepare the standard concentration of limonene for 0.2 ppm solution from the
stock solution with series of dilution with pentane.
Measure the peak area of the standard samples using GC.
Draw the linear curve for the standard samples utilizing the peak area reading
against each concentration.
Calculate the linear equation from the plot. Y = mX +c.
Calculate the unknown concentration of limonene utilizing the linear equation of
the standard samples.
Amalina Syahirah
151141187
Amalina Syahirah
151141187
PREPARATION OF SAMPLE
1.
2.
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3.
4.
Transfer the extract to a 50 ml beaker wraped with Aluminium foil and remove
all pentene using water bath at 35oC in a fume hood for 15 minutes.
35oc
5.
For the chromatographic analysis, dilute the citrus oil with 1.0 ml of pentane and
inject 1.00 l of the resulting solution into the GC.
bAmalina Syahirah
151141187