Summary

Contained this document, the images and text outline the specimens observed
showing continuum of the specimens from initial observation to more complex
slides.. In the beginning, the letter e, crossed hairs, and rulers was examined to
help learn how to use the microscope. Then, a diverse gallery of cells were
observed. These included oral cheek, onion, and amoeba cells. The final aspect of
this lab was the observation of diffusion within cells.
Intro:
The Letter e- The e is upside down because under the microscope the e was rightside up.

Newspaper
scanning 40x

1200 m

Crossed Hairs:

Human hair
Scanning 40x

100 m

Ruler:

Ruler piece
Lower power 1600x

320 m

Analysis
1. Summarize the differences an image through a microscope and the same
image viewed with the naked eye
a. The difference between a microscope an image viewed the naked
eye is that in a microscope the image is upside down. For example,
when viewing the letter e the top of the e is on the bottom while
when viewing with the naked I the top of the e is on the top. Under
microscopes, the image is rotated.
2. When viewing an object through the high power objective not all of the
object may be in focus? Explain
a. In the highest power objective, the microscope zooms in a small part
of the object. However, anything that is not in this small part will
not be in focus. Therefore, some objects might not be focused under
the highest power objective
3. What is relationship between magnification and the diameter of the field
of view?
a. When the magnification increase, the diameter of the field
decreases. As the magnification increases, the microscope focuses
on a smaller part of the object. As the microscope focuses on that
smaller portion, the diameter decreases
4. What is diameter in micrometers of the low power field of view on your
microscope?

a. The diameter of the low power if 1600 micrometers. This is because

1 millimeter is 1000 micrometers and the diameter is 1.6 millimeter.
You would do 1.6mm x 1000 mu=1600mu
5. Calculate the diameter in micrometers of you high power field.
a. 400 mu using equation: 40/10=4 1600/4=400
Gallery of Diverse Cells
Onion Cell: Vegetable/ Low Power 100x: 400 m

Elodea: Low Power 100x/100 m

Cheek Cell-Cheek Cell/ High Power 400x/ 57 m

Human ground bone- Human bone/ High Power 400x/13 m

Human Skeletal Muscle: Muscle/ High Power 400x/3 cm

Paramecium: Paramecium/ High Power 400x/ 450m

Amoeba: Amoeba/ High Power 400x and Oil 125x/ 300 m

Saccharomyces (yeast)/ Lower Power 100x/ 5 m

Euglena: Euglena/ Oil: 125 x/ 75 m

Volvox: Volvox/ High Power 400x, Oil 125x/ 4m

Diffusion within Cells

Potato Cell with No Iodine
Going to be translucent
Must be sliced thin enough
Remember to look around the
edges if the potato is too thick
With water

Potato Cell with Iodine

Cells will turn purple, black, or
brown
The whole potato will not turn that
color