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Kazem Naddafi1 , Ramin Nabizadeh2 ,

Masoud Yonesian2 , Gholamreza Jahed2 , Ayoub Beyki3

Abstract

Accepted Jul. 2, 2006)

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(Received Sep. 25, 2005

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The purpose of this study is to determine arsenic toxicity on rainbow trout. Acute toxicity of arsenic
was determined by measuring the lethal effects on rainbow trout in static conditions. Five aquariums
of 253030 cm with five concentrations of 5,10,15,20 and 25 mg/L of arsenic were prepared and
then ten fishes were added to each concentration. Also one aquarium with similar conditions was
considered as a control with no arsenic solution. Hardness, temperature and dissolved oxygen of
dilution water were determined by standard methods, and concentration of dissolved oxygen, pH and
temperature of solution test in time periods of 2,4,6,8,24,48,72 and 96 hrs were measured. Water
temperature of aquarium was regulated by circulation of water in refrigerator through indirect
conduction with solution test. LC50 was measured at intervals of 24,48,72 and 96 hrs by SPSS software
and respectively showed 28.13,21.77,15.78 and 12.72 mg/L.Probit curve was drawn by Harvard Chart
XL software, and LC50 curve was drawn by Excel software.
Keywords: Rainbow Trout, Arsenic Toxicity,Water Pollution.
1-Associate Prof., School of Public Health, Tehran University
of Medical Sciences, knadafi@tums.ac.ir
2- Assistant Prof., School of Public Health, Tehran University
of Medical Sciences
3-M.Sc. Student, School of Public Health, Golestan University
of Medical Sciences

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1- Clesceria, L. S., Greenberg, A.E., and Eaton, A. D., eds. (1998). Standard methods for the
examination of water and wastewater, APHA, WEF, AWWA, 20th Ed., Washington, D.C.
.^ R 7 V 5
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=* . $U ( ^~

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3- OECD. (1992). Guidelines for testing of chemicals, Section 2: Fish acute toxicity test.
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12- Chung-Min,L.,and Ming-Chao, L. (2001). Acute toxicity modeling of rainbow trout and silver
sea bream exposed to waterborne metals. J. Environmental Toxicology, 16(4), 349-360.
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aquatic invertebrates, 1st Ed., United States Department of the Interior Fish and Wildlife
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