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Aileen Aquino

12/15/16
Enzyme lab report
Ms. Lenowitz

Can changing the magnetic stirring speed increase the rate of


reaction?

Introduction:
Enzyme catalase and the substrate (H2O2) combine in the active site and then the
enzyme catalase breaks it down into H2O (Water) and O2 (Oxygen). This process is called
digestion. In general enzymes are proteins that speed up reactions like synthesis and digestion,
and in this experiment we are using catalase which is an enzyme. And a substrate is what
attaches to the enzymes and two things can happen to the substrate, it can either be digested
(broken down) or synthesized (Put together). In this experiment the substrate is H2O2
(Hydrogen Peroxide + Oxygen) and it is being broken into O2 (Oxygen) and H2O (Water). In
this lab we measured the amount of O2. We had a control and experimental test. In the
experimental test we increased the speed of the magnetic stirrer. We thought if we increased
the speed of the magnetic stirrer ten the rate of reaction will increase as well because it's
stirring quicker meaning the catalase is moving so possibly the substrate can synthesize.

Procedure:
Catalase Lab Procedure
1. _____all_____Check that you have all the equipment below.
Computer with Internet access and Vernier LoggerPro software
LabQuest Mini
Vernier Gas Pressure Sensor
Two-hole black rubber stopper (top)
Plastic tubing with Luer-lock connectors
20-200 L micropipette set to 100 L
micropipette tips
50mL graduated cylinder
125 mL or 250 mL Erlenmeyer flask
Magnetic stirrer
Stirring bar
Thermometer

Metal clamp stand


At the central table:
Hydrogen peroxide
Catalase enzyme suspension
2. ____all______Check that every person is wearing apron and goggles.
3. ____all______Check that everyone who will touch the enzyme or liquids is
wearing thick latex gloves (blue and yellow gloves)
On the blank lines, write the initials of the teammate who completed the task:
4. __________Open your laptop and open Logger Pro .
5. __________Click File Open and open the folder in Biology by
Vernier. Then choose 06 Enzyme (Pressure).
6. __________Connect the LabQuest Mini to the computer using the USB
cable, and connect the Gas Pressure Sensor to CH 1 of the LabQuest Mini.
Set up the laboratory apparatus as seen in the picture:
7. __________Measure out 50mL of 1.5% H2O2
and pour into an Erlenmeyer flask.
8. __________Carefully place a magnetic stir bar in
the flask.
9. __________Place the flask on a magnetic stir
plate. Use a clamp to fasten the flask to the ring
stand as shown. Position the flask at the center
of the magnetic stirrer.
10. __________Test the stirrer at 100 rpm.
11. __________Stop the stirrer.
12. __________Use the plastic tubing with two Luerlock connectors to connect the two-hole rubber
stopper assembly to the Gas Pressure Sensor as shown in the image. The
valve connected to the stopper should stay closed during this investigation.
Its closed when its flat, parallel to the floor
Complete all the steps below quickly to complete your test reaction.
13. __________Start data collection: click green Collect button on Logger Pro.
14. __________Using a micropipette, add 100 L of enzyme suspension to the
contents of the flask.
15. __________IMMEDIATELY tightly seal the flask by placing the stopper in and
HOLDING it in carefully.
16. __________IMMEDIATELY Turn the stir plate on to 100.
NOTE: If the pressure exceeds 130 kPa, the pressure inside the flask will be
too great and the rubber stopper will likely pop off. HOLD DOWN the
stopper or be ready to have uncovered chemicals on your desk
WAIT 200 seconds (3.3 minutes) while data is
collecting- Do NOT click STOP. Data collection will automatically stop

after 200 seconds.


17. __________Turn off the stir plate.
18. __________Carefully remove the stopper from the flask to relieve the
pressure.
19. __________Use a thermometer to test and record the temperature of the
liquid in your lab packet
20. __________Pour all chemical waste into a RED bucket.
21. __________Remove the magnetic bar.
22. __________ Dispose of your used micropipette tip.
23. ___all_______Take off your safety gear and place it neatly away.
24. Observe the graph generated using LoggerPro software (example shown
below).
25. Hold down Control on the keyboard and press j to zoom in the graph.
26. Highlight the section of the graph where the slope is increasing, by clicking
and dragging your mouse across it.
27. Click the Analyze tab at the top of the page, and choose Linear Fit. A
statistics box will appear for your highlighted section of the graph.
28. Record the slope of the line, m, as the rate of catalase activity in kPa/s in
your lab packet (page 13)
29. Click File, Save As, and save with the file name:
(Your Names) Sam, Anabel, Jenni, Jose Control Trial Logger Pro Data
Email the graph to Ms. Lenowitz by following these final steps!
30. Click File and choose Print Graph. In the drop down menu of printers, choose
Cute PDF Writer in the drop down list. This will export your graph as a
PDF file. Be patient- it takes a few seconds to pop up.
31. Save the PDF with the this file name:
(Your Names) Sam, Anabel, Jenni, Jose Control Trial Data PDF
32. Open your MESA email and Compose a new email to
glenowitz@mesacharter.org
33. Attach the PDF to the email.
34. Press send!

Analysis:
My hypothesis was that if we increased the rate of the magnetic stirrer, than the rate of
reaction will increase, and our results did not support our hypothesis because the rate of
reaction decreased. The rate of reaction for the control was m=142.7 kPa/min and for the
experimental it was m= -13.17. Our data is pretty odd but even if the experimental trial worked it
probably wouldnt have made a better change because the variables that change enzymes are
its pH or temperature and we changed neither.

Conclusion:
There were a few errors. We had to try our experimental trial twice. The first time the
magnetic stirrer was much too big and in the second the luer-lock connectors werent placed in
tight enough. We probably shouldve checked before we started if everything was placed in
tight. In conclusion our experiment shows that the speed of the magnetic stirrer did not increase
the rate of change of the catalase but decreased it.