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INTRODUCTION
Squamous cell carcinoma of head and neck (SCCHN)
is globally the sixth most common cancer,which
includes cancer of oral cavity, larynx, and pharynx.
In developing countries especially in south Asia,
there are growing habits of consumption of tobacco
and its products in the form of surti, bidi, cigarette,
khaine, and pan masala. The tobacco and tobacco
products are known to generate a strong free radical
environment, when the free radicals overwhelm the
antioxidant system, they may lead to degeneration
of cellular components and mutations.
Free radicals are the molecules which contain
an unpaired electron in their outer shell and are
generated endogenously during various cellular
metabolic activities and exogenously by number
of harmful compounds, tobacco and tobacco
products. In human cells, mitochondria are the
major intracellular source of reactive oxygen species
(ROS) generation.[1,2] These molecules are highly
254
Anil Kumar,
Mohan C. Pant1,
Hirdya S. Singh2,
Shashi
Khandelwal
Immunotoxicology
Division, CSIR, Indian
Institute of Toxicology
Research, 1Ch. S.M.
Medical University,
Lucknow, 2Ch. Charan
Singh University
Meerut, India
For correspondence:
Dr. Shashi
Khandelwal,
Scientist F and
Head, CSIR Indian
Institute of Toxicology
Research, 80 Mahatma
Gandhi Marg,
Lucknow 226001,
India.
E-mail: skhandelwal_
itrc@rediffmail.com
51
2560 years
84
6
32
14
38
N = 100
53
2575 years
93
7
34
15
44
54
46
255
RESULTS
The overall profile of TAC, GSH, RNS and ROS of controls
and SCCHN patients is given in Figure 1. Total antioxidant
capacity of the patients as evaluated in blood was found to be
substantially suppressed in comparison to controls. The TAC
value in blood of controls was 1.63 M, whereas in the SCCHN
patients, levels dropped to 1.40 M. A ~1.2-fold reduction
(P < 0.001) in TAC was evident in the plasma.
Similarly, GSH levels in both saliva and blood of SCCHN patients
indicated a lowering pattern. A ~1.7-fold reduction in GSH
suggests an oxidantantioxidant imbalance in the cancer patients.
GSH values in blood fell to 4.40 M from 2.54 M in controls.
In contrast, ROS and RNS were found to be elevated in SCCHN
patients. The NO2 levels in blood, increased by 1.7-fold. The
NO2 values were 62.68 M when compared to 37.21 M in
controls. The ROS values in blood also showed a substantial
~3.0-fold increase. Control ROS levels of 3.20 M increased to
9.50 M in the patients.
0 2
62.68 6.94
3.20 0.35
2.54 0.38
9.20 0.57
***
15. 00 1.64
**
9.50 0.92
37.21 4.65
***
4.40 0.52
1.40 0.23
1.63 0.28
4 6 8 10 12 14 16 18 40 50 60 70 80
***
Figure 1: Plasma TAC, GSH RNS and ROS in controls (n=90) and
HNSCC patients (n=100), 8-OHdG in blood cells of controls (n=50) and
HNSCC patients (n=50),The level of TAC is expressed as mM, GSH,
RNS and ROS as M, 8-OHdG as number of apurinic sites/105 bp.
Pearsons
coefficient
0.38**
0.44**
P-value
0.01
0.01
Table 3: Levels of the plasma oxidative stress determinants in controls and SCCHN cases
Non-habituates
Control
Case
1.83 0.10 1.50 0.17
5.12 0.48 3.16 0.95
2.49 0.65 8.47 1.02
31.74 0.30 40.09 1.28
5.83 0.39 9.11 0.21
Smokers
Control
Case
1.67 0.03* 1.24 0.02*
4.40 0.52* 2.48 0.61*
3.24 0.72* 9.22 1.44*
35.98 7.54* 65.17 3.80*
7.33 0.71 13.28 2.36
Chewers
Smokers + chewers
Control
Case
Control
Case
1.55 0.03
1.26 0.02 1.48 0.04* 1.33 0.05*
4.06 0.77* 2.37 0.78* 3.87 0.42* 2.33 0.47*
3.35 1.39* 9.97 1.61* 3.87 0.88* 10.71 1.42*
38.43 2.37* 66.77 14.34* 43.33 3.01* 76.67 8.18**
9.55 0.77 14.12 1.59 10.84 0.88 16.08 2.82
257
REFERENCES
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
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