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Uniquechallengesandfuturedirectionsrelatedto
managingmousegnotobiotic husbandryfacilities
ChrissJ.Vowles
UniqueChallengesandFuture
DirectionsRelatedtoManagingMouse
Gnotobiotic HusbandryFacilities
Challengesingermfreecolonymanagement
Nationalstandards
Germfreeanimalhealth
Accesstogermfree/gnotobiotic animals
Staffing
Funding
FutureDirections
Research
TraditionalExperimentalHousing
AlternativeBioexclusion System
FunctionandPurpose
Maintainagermfree
animalcolony
Husbandry
Colonymanagement
Gnotobiotic Research
Allocationofmiceto
experimentalspace
Collaboratewith
investigator
Design
Executionofexperiment
NationalStandards
LackofGnotobiotic
guidelines
Termgnotobioticonly
seenonpage52,70,and
135oftheGuideforthe
CareandUseof
LaboratoryAnimals,8th
edition
AnimalUseCommittee
Concerns
Physicalcagechanges
Dirtyisolator
GermfreeAnimalHealth
Abnormallylargececum
Occasionallyleadstogut
twist
Nutrition
Heatdegradationtofeed
Thiamine
VitaminK
Supplements
Irradiation
Sterilityoftenunreliable
AccesstoAxenic/Gnotobiotic Animals
CoreTechniciansand
VeterinaryStaffOnly
Veterinarystafftraining
Guided
Treatment
Sterile/new
Externallysterilize
packaging
Staffing
Lackofexperiencedstaff
Sixmonthsoftraining
Fatigue
Highstress
Nomistakes
Personalitytraits
Highattentiontodetail
Ergonomicburden
Funding
Veryexpensive
Space
Labor
Unrealisticforthecoretosupportitself
Alternativesourcesoffundingneeded
Staffsalaries
Specializedtraining
Nostandardforcompensation
Experiments
UserRequestForm
Investigatorfillsout
requestform
Staffapprovesrequest
Consultation
Scheduleexperiment
Gnotobiotic ExperimentalHousing
SoftsideIsolators
Pros
BasicPPE
Plasticgown,gloves
Lowriskofcontamination
Large,experimental
populationoptions
Cons
Onlyonemicroorganismat
atime
Crosscontaminationis
unavoidable
24houradvancedplan
Largefootprint
Gnotobiotic ExperimentalHousing
ClassIIBiosafetycabinet
Strictasepsis
Surgicalsterility
Pros
Easyaccess,withinminutes
Gooddexterity
Usebasic,staticcaging
Cons
Limitedspace
Highriskofcross
contamination
Largefootprint/lowmouse
density
HermeticallySealed,
IndependentlyVentilatedCages(IVC)
Tecniplast
Isocage P
HermeticallySealedCages
SmallIsolators
Allentown
SPP
AsepticManipulationBetween
Gnotobiotic Groups
SterilizeCabinet
Cage
Decontamination
Manipulate
SterilizeCage
AsepticallyDon
SterilePPE
SterilizingtheCabinet
Sterilize
Cabinet
CageDecon
Manipulate
SterilizeCage
Aseptically
Donsterile
PPE
SterilizetheExterioroftheCage
Sterilize
Cabinet
CageDecon
Manipulate
SterilizeCage
AsepticallyDon
sterilePPE
AsepticallyDonninginSterilePPE
Sterilize
Cabinet
CageDecon
Manipulate
SterilizeCage
AsepticallyDon
sterilePPE
Manipulation
Sterilize
Cabinet
CageDecon
Manipulate
SterilizeCage
AsepticallyDon
sterilePPE
CageDecontamination
Sterilize
Cabinet
CageDecon
Manipulate
SterilizeCage
AsepticallyDon
sterilePPE
IVCLimitations
ChlorineDioxideSterilant
(Exspor)
Contacttime
Toxicity
Corrosive
Time
Materials
Labor
Crosscontaminations
Rotatingcabinets
Maintenance
Valves
Springs
Plastic
CansealedIVCunitsreplaceisolators?
No
Isolatorsaresafertohousetheaxenic
colony
HermeticallysealedIVCsgreatlyincrease
efficiencyofexperimentation
Highriskofcontaminationdueto
individualhusbandryneeds
AdvantagesofHermeticallySealed
Cages
Nine,classIIbiosafetycabinets
Roughly2025cages
Maximumof9 experiments
456sq ft room
Replacedbyone,71cagedoubleunit
Norealisticgrouppopulation
restrictions
2014 completed84
2015completed120experiments
2016topresent over130
IncreasedFunding
Moreuserfeesgenerated
Summary
Challenges
Nationalstandards
Funding
Futuredirections
Alternativesterilant
HermeticallysealedIVCisamajoradvancementin
gnotobiotic experimentation
ThankYou!
NASILAR
Dr.KathrynEaton
SaraPoe
NatalieE.Anderson
TrishaJDenike