Multiplex Paper Biochip for

Acute Kidney Injury

Group 31
Presented by
Benjamin Huang
450,000+
Estimated people affected annually by AKI

50%
Of diagnoses result in death, depending on severity

$15,900
Estimated increase in cost of care due to AKI
 Changes to Project

Resource-Limited Design Timeline Team Responsibility

Specifications
Biochip Output
Sensitivity &
Selectivity
Design Alternatives
Hydrophobic
Bioink
AuNR Length
Nanostructure
Geometry
 Antibodies

ARTIFICIAL VS. REAL

Cost

Stability

Accuracy
 Other
Considerations

r
SC
Pugh Chart
 Chosen Solution

-3D print template -Limited time for project

-Relatively simple -Difficult fabrication
fabrication after designing -Higher cost
-Low Cost -Shorter testing time
-Longer testing time
 Budget

Item Quantity Cost Source

Precision X-Acto 2 $11.78 Washington
Knife University
Bookstore
X-Acto #11 100 $19.44 Amazon
Replacement Marketplace
Blades
Total Cost: $31.22
 Sources
Figure sources listed in notes of each slide.
General sources for information:
1. Malyszko J, Lukaszyk E, Glowinska I, Durlik M. Biomarkers of delayed graft function as a form of acute kidney injury in kidney transplantation.
Nat Publ Gr. 2015. doi:10.1038/srep11684.
2. Tian L, Liu K-K, Morrissey JJ, Gandra N, Kharasch ED, Singamaneni S. Gold nanocages with built-in artificial antibodies for label-free plasmonic
biosensing. doi:10.1039/c3tb21551b.
3. Aydogdu M, Grsel G, Sancak B, et al. The use of plasma and urine neutrophil gelatinase associated lipocalin (NGAL) and Cystatin C in early
diagnosis of septic acute kidney injury in critically ill patients. Dis Markers. 2013;34(4):237-246. doi:10.3233/DMA-130966.
4. Martin LJ, Zieve D, Ogilvie I. Creatinine Urine Test. In: Medline Plus. US National Library of Medicine; 2015.
https://medlineplus.gov/ency/article/003610.htm.
5. Tian L, Morrissey JJ, Kattumenu R, Gandra N, Kharasch ED, Singamaneni S. Bioplasmonic paper as a platform for detection of kidney cancer
biomarkers. Anal Chem. 2012;84(22):9928-9934. doi:10.1021/ac302332g.
6. Warren AD, Kwong GA, Wood DK, Lin KY, Bhatia SN. Point-of-care diagnostics for noncommunicable diseases using synthetic urinary
biomarkers and paper microfluidics. Proc Natl Acad Sci U S A. 2014;111(10):3671-3676. doi:10.1073/pnas.1314651111.
7. Abbas A, Tian L, Morrissey JJ, Kharasch ED, Singamaneni S. Hot spot-localized artificial antibodies for label-free plasmonic biosensing. Adv Funct
Mater. 2013;23(14):1789-1797. doi:10.1002/adfm.201202370.
8. Martinez AW, Phillips ST, Butte MJ, Whitesides GM. Patterned paper as a platform for inexpensive, low-volume, portable bioassays. Angew Chemie
- Int Ed. 2007;46(8):1318- 1320. doi:10.1002/anie.200603817.
9. Tian, L. et al, Bioplasmonic calligraphy for multiplexed label-free biodetection, Biosensors and Bioelectronics, Volume 59, 15 September 2014,
Pages 208-215
10.Yu C, Irudayaraj J. Multiplex biosensor using gold nanorods. Anal Chem. 2007;79(2):572- 579. doi:10.1021/ac061730d.
11. Wang W, Wu WY, Wang W, Zhu JJ. Tree-shaped paper strip for semiquantitative colorimetric detection of protein with self-calibration. J
Chromatogr A. 2010;1217(24):3896-3899. doi:10.1016/j.chroma.2010.04.017.
12. Dungchai W, Chailapakul O, Henry CS. Use of multiple colorimetric indicators for paper- based microfluidic devices. Anal Chim Acta.
2010;674(2):227-233. doi:10.1016/j.aca.2010.06.019.
13.Abbas A, Brimer A, Slocik JM, Tian L, Naik RR, Singamaneni S. Multifunctional analytical platform on a paper strip: Separation, preconcentration,
and subattomolar detection. Anal Chem. 2013;85(8):3977-3983. doi:10.1021/ac303567g.
14. Luan J, Liu K-K, Tadepalli S, et al. PEGylated Artificial Antibodies: Plasmonic Biosensors with Improved Selectivity. ACS Appl Mater Interfaces.
2016;8(36):23509-23516. doi:10.1021/acsami.6b07252.
15. Rose F. Ueber die Verbindungen des Eiweiss mit Metalloxyden. Ann der Phys und Chemie. 1833;104(5):132-142. doi:10.1002/andp.18331040512.
16. LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem.
1951;193(1):265-275. http://www.ncbi.nlm.nih.gov/pubmed/14907713. Accessed December 2, 2016