GENETIKA MOLECULAR SERVICES

PCR OPTIMIZATION ORDER FORM

CUSTOMER DETAILS
Name
Ref ID
Institute name/department
Address

Contact number (office) : (mobile) :

Email address

Type of Service (Please choose ONE only)

Please Indicate
Service Name Description
with a x
SERVICE A PCR optimization WITH Primer Design Primer will be designed and synthesized at Genetika Science
SERVICE B PCR optimization WITHOUT Primer Design Primer sequences will be submitted by customer
 GENETIKA MOLECULAR SERVICES
PCR OPTIMIZATION ORDER FORM

SERVICE A PCR optimization WITH Primer Design

Target DNA Template
Type of Sample
No GenBank gDNA
Region No. Conc. Purity Vol.
. Accesion Name Size (bp) Name Organism cDNA
Start...End (ng/L) OD 260/280 (L)
Number Fresh
Culture
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
 GENETIKA MOLECULAR SERVICES
PCR OPTIMIZATION ORDER FORM

SERVICE B PCR optimization WITHOUT Primer Design

PCR Primers DNA Template PCR Product
Type of Sample
No gDNA
Primer Name Tm No. Conc. Purity Vol.
. Name Organism cDNA Size
Sequence (5 to 3) (C) (ng/L) OD 260/280 (L)
Fresh
Culture
Forward : 1.
Intersequence : 2.
Reverse : 3.
Insert sequence : 4.
5.
Forward : 1.
Intersequence : 2.
Reverse : 3.
Insert sequence : 4.
5.
Forward : 1.
Intersequence : 2.
Reverse : 3.
Insert sequence : 4.
5.
 GENETIKA MOLECULAR SERVICES
PCR OPTIMIZATION ORDER FORM

Add-on Services (NOTE: Additional fees are applicable, please submit DNA sequencing order form or PCR Production Cloning
submission form separately
Please Indicate with a x Types of service Quantity
DNA Exttraction
RNA Extraction
cDNA Synthesis
PCR Product Cloning
Sequencing of PCR Product
Blunting of 3d A-overhang PCR Product

Optional: If PCR Optimization has been done before, please attach PCR Setup, Thermal cycling Condition, labelled gel photo for our reference