pBLU Lab Results

LB - LB +

LB/Amp/X - LB/Amp/X +
Conclusion Questions:

1. Justify the importance of each

Calcium chloride The plasmid
Luria broth Temperature of the Incubator
Ampicillin Nutrient Agar
Water bath (warm) Restriction Enzymes
Ice bath before heating Sticky vs Blunt ends
Ice bath after heating E. coli
Xgal

2. Explain the purpose of each of the 4 3. Explain the results from each of the 4
plates. plates.
a. LB+ a. LB+
b. LB- b. LB-
c. LB/Amp/Xgal+ c. LB/Amp/Xgal+
d. LB/Amp/Xgal- d. LB/Amp/Xgal-

4. After the 2nd day, one of the groups had blue colonies surrounded by several little white colonies
as shown on the right. What are two possible reasons that would explain why there are white
colonies are present around the mother colony.

5. Explain one sources of error that your group had in this lab and EXPLAIN why the error caused a
problem.