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IN CYTOTOX XENOMETRIX

by Endotell

NR - CVDE
APPLICATIONS
Combined colorimetric assays for the quantification of the lysosomal activity and for the
quantification of nuclear DNA and cell number in response to pharmaceutical, chemical
and environmental compounds, and nutrients.

PRINCIPLE
This kit allows to measure sequentially two cytotoxicity parameters in one single cell
culture: Lysosomal integrity and activity (NR: Neutral Red) and nuclear DNA and cell
number (CVDE: Crystal Violet Dye Elution).
Neutral Red is taken up and stored in the lysosomes of viable cells and is a marker for
membrane integrity and lysosomal activity. Crystal Violet is a dye that accumulates in
the cell nucleus. The fixed dye, measured photometrically after solubilization, correlates
with the nuclear DNA content and thus with cell number.

BIOLOGICAL PARAMETERS EVALUATION


IC50 (Inhibitory Concentration 50%) Nuclear DNA
Lysosomal activity Cell proliferation

CVDE staining of mouse L929 cells

1.0000
0.9000
0.8000
0.7000
OD540-OD690

0.6000
0.5000
0.4000
0.3000
0.2000
0.1000
0.0000
Medium 1

40000

20000

10000

5000

2500

1250

625

312

156

78

Medium 2

Cells per well


TECHNICAL SPECIFICATIONS
Absorbance: - NR: 540 nm
- CVDE: 540 nm

Approximate assay time (total): - 4 hr 20 min plus time to dry the plate

Available kit configurations: - Reagents only


- Reagents and 96-well microplates, sterile
reagent reservoirs

RN - CVDE Kit content: - Wash solutions


- Labeling solutions
- Fixing solution
- Solubilization solutions
- Instruction manual

NR - CVDE KIT
SIZES AND REFERENCE NUMBERS
REFERENCE NO. NUMBER OF TESTS
KRCV96.300 2 x 300
KRCV96.1200 2 x 1200

Kits with plasticware (microplates and reservoirs) are also available.


Individual reagents and other kit sizes available upon request.

Version 1.5 01/2007

Xenometrix
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CH-4123 Allschwil
Switzerland
Tel.: + 41 61 482 14 34
Fax: + 41 61 482 20 72
Email: info@xenometrix.ch
Web: www.xenometrix.ch