NON-FERMENTING GRAM Swimmers ear

NEGATIVE BACILLI Jacuzzi/ Hot tub

Syndrome
Non-Fermenting Gram Negative Bacilli
Severe Pneumonia
gram negative bacilli among CF patients
aerobic VIRULENCE FACTOR
Fail to acidify of Media that is LPS
overlaid with mineral oil and TSI Unusual Porin Channels
Ubiquitous found in most Pili
environments (soil, plants, Capsule (Aiginate)
decaying vegetation, isolated in Elastase
hospital equipment and other Exotoxin A
devices) Hemolysin
can withstand treatment with Pyocyanic Pigment
disinfectants
*Rx of Choice: Aminoglycosides,
resistant to most antimicrobials
Piperacillin, Ticarcillin, Ceftazidine,
A. Family Pseudomonadaceae Cefepime

Pseudomonads LABORATORY DIAGNOSIS

Produces Pigments
Generalities of Medically Pyocyanin
important members Pyoverdin
Gram Negative Bacilli/ (Pyocyanin +
coccobacillus Pyoverdin = Green
Strict Aerobes Colonies)
Motile Other Pigments:
Oxidase (+) except P.luteolus pyorubin and
pyomelanin
and P.oryzihabitans
Odor: Grape Like or
Catalase (+)
Tortilla like
Most are able to grow in
Temperature: Ability to
MacConkey Agar
grow at 47C
Most are oxidizers, some are
Motile
assacharolytic ON CULTURE AND
1. Pseudomonas aeruginosa BIOCHEMICAL MEDIA
BAP-large, rough, dull
Most frequently isolated species grayish spreading
Important opportunistic colonies with feathery
pathogen among edge (ground glass
immunocompromised appearance)
Common cause of nococomial NA and MHA-pigments
infections are visible
Associated diseases: EMB-colorless colonies
Bacteremia, skin, wound Cetrimide Agar- positive
infections, UTI, endocartis growth
 BIOCHEMICAL REACTIONS
Oxidase (+)
Oxidizer (Glucose,
Fructose, Xylose)
LDC (-); ODC and ADH (+)
Indolde (-)
Gelatinase (+)
NO3 reducer
Citrate (+)
Acetamide Utilization (+)
Other Pseudomonas species
2. Pseudomonas fluorescens
3. Pseudomonas putida
Normal Flora: Opportunistic
organisms
Low virulence
Associated with: UTI, post
surgical, abscesses, empyema,
septic arthritis
Growth at NO NO
42c
4. Pseudomonas stutzeri
Diseases: otitis media, septic
arthritis, pneumonia among CF
patients, meningitis among HIV
patients, UTI
Colonies: Dry wrinkled with
buffy/light brown color (tough
and adherent)
5. Pseudomonas mendocina
Disease: Endocardities; usually
a contaminant
Colonies: Non wrinkled with
bufi/light brown pigment
*Soil Denitrifiers: use ammonium as
sole nitrogen source

IDENTIFICATION: *Can grow in Anaerobic condition

P.fluoresce P.putida P.stutzeri P.mendoci

ns na
Pyocyanin NO NO ADH (-) (+)
Pyoverdin YES YES Starch (+) (-)
Gelatinase (+) (-) Hydrolysis
NO3 (-) (-)
reducer 6. Pseudomonas
Xylose YES YES pseudoaicaligenes
Oxidizers
ADH (+) (+) 7. Pseudomonas alcaligene
LDC and (-) (-)
ODC Usually contaminants
Growth in NO NO Oxidase (+), able to grow on
Cetrimide MacConkey; motile
Acetamide NO NO P.pseudoalcaligene = ADH (+)
Utilization