Biology

Osmosis Lab Report

Chatthanayongphakdi, Natthapat,
Pond

Wachapatthana, Ingkarat, Ing

Singlamlert, Chomphunik, Fay

Petchpadoong , Thitaporn,
Genie

Tavichai, Chinorod, Chino

1004

Date : March, 10th 2017

Introduction: attached in the back

Purpose: To observe the effects and changes of objects due to the process of
osmosis by its hypotonic and hypertonic features.

Question: How will soaking the Jelly in distilled water affect the size of the
candy?
Hypothesis: If the jelly is in distilled water, then the size of jelly will be
bigger after being soaked.
Question: How will soaking the Jelly in concentrated salt water affect the
size of the candy?
Hypothesis: If the jelly is in salt water, then the size of jelly will be smaller
after being soaked.

Question: How will soaking the Potato in distilled water affect the size?
Hypothesis: If the potato is in distilled water, then the size of potato will be
bigger after being soaked.

Question: How will soaking the Potato in concentrated salt water affect the
size?
Hypothesis: If the potato is in salt water, then the size of potato will be
smaller after being soaked.

Materials:
6 Beakers (3 for jelly, 3 for Concentrated salt water
potato) 3 Jellies
Electronic Balance 3 Potato Pieces
Distilled water Calculator groups bring their
 own calculator Weighing paper
Markers
Graduated cylinder
Knife Lab Coat
Goggles
Methods:
Collect the materials that your group needs (3 Jelly and Half a Potato)
Use the knife to cut the potato into 3 equal pieces
Use the electronic balance to find the mass of your jelly. Remember to lay down
a piece of weighing paper and set to zero.
Record your data
Record descriptive observations about the jelly prior to the experiment.
Measure the initial volume of the jelly and the potato and record your data.
Fill your 1st beaker halfway with distilled water. Put one of your jelly into the
beaker. Set the beaker aside for one hour. Label the beaker using marker.
Fill your 2st beaker with concentrated salt solution halfway. Put your 2nd jelly into
that beaker. Set beaker aside for one hour. Label the beaker using marker.
Put your 3rd jelly into an empty beaker without putting anything in it. That should
be your control.
Put the 4th beaker halfway with distilled water. Put one of your potato pieces into
the beaker. Set the beaker aside for one hour. Label the beaker.
Put the 5th beaker halfway with concentrated salt solution halfway. Put your
second potato pieces into that beaker and set it aside for an hour. Label the
beaker.
Place your last piece of potato pieces into the last beaker, empty. That is your
control for the potato. Label the beaker.
Make sure you label all the beakers distilled water or concentrated salt
solution with some markers.
After one hour, come back and look at each of the beakers and record the data
volume and mass of both the jellies in two beaker and both the potato in two
other beakers.
Also record the descriptive data for what the jelly and the potato look like now
after being soaker for an hour.
Calculate the percent change in the size of each jelly and potato
a.Percent change in volume
b. Percent change in mass

DATA TABLE A:

Jelly 1 (Color): Jelly 2 (Color):

Dimensions Before After Before After soaking

Soaking Soaking in Soaking in Salt Water
Distilled
Water

Volume 2.0ml 3.0 ml 1.8ml 1.0 ml

Mass 2.66g 4.17g 2.90ml 1.56 g

DATA TABLE B:
 Potato 1 (Yellow): Potato 2 (Yellow):

Dimensions Before After Before After soaking

Soaking Soaking in Soaking in Salt Water
Distilled
Water

Volume 4.0 ml 5.0 ml 4.0 ml 3.0 ml

Mass 4.10 g 4.78 g 4.31 g 3.54 g

DATA TABLE C:

Jelly 1: (Red) Jelly 2: (Red)

Descriptive Observations Before Descriptive Observations Before

Soaking in Distilled Water: It is in Soaking in Salt Water: It is in
semicircle shape. It is soft and sticky semicircle shape. It is soft and sticky
in the same time and it smell good in the same time and it smell good

Descriptive Observations After Descriptive Observations After

Soaking in Distilled Water: The Soaking in Salt Water: The jelly
jelly will swell because more water will shrink because more water go
go into the jelly to make the balance out of the jelly to make the balance
of concentration of concentration in the environment.

DATA TABLE D:

Potato 1: (Yellow) Potato 2: (Yellow)

 Descriptive Observations Before
Soaking in
distilled water : The potato is slice
into dice shape, hard feeling and
slippery.
Descriptive Observations Before
Soaking in salt
water : The potato is slice into dice
shape, hard feeling and slippery.

Descriptive Observations After Descriptive Observations After

Soaking in Di distilled water :
The potato become bigger and th it have
a lighter colour. Because the potato is hy
hypotonic to the distilled water so the
water go transport into the potato.
Soaking in salt
water : The potato is yellow color,
dice shape, sm smooth surface
texture. The potato is hypotonic
comparing to the salt water which is
hypertonic It also getting smaller.

DATA TABLE E:

Jelly 1 (Red): Jelly 2 (Red):

Soaked in Distilled Soaked in Salt
Water Water

Percent Change in +50 % -44.4%

Volume

Percent Change in + 56.8% -46.2%

Mass

DATA TABLE F:

Potato 1 (Yellow): Potato 2 (Yellow):

 Soaked in Distilled Soaked in Salt
Water Water

Percent Change in + 25% -25%

Volume

Percent Change in +16.6% -17.9%

Mass

Bar Graph:
Make bar graphs to
show the percent
change in mass and
volume for both the
jelly and the potato.

Analyzing
Results Questions:
1. What happened to
the jellies after
being soaked in
distilled
water and salt
water for an hour?
Why? Be
descriptive
and detailed in your answer. Use your knowledge of membrane transport.
- After two jellies are being soaked in the solution for an hour, it
changes the
size of its
shape. For
jelly in the
distilled
water, the
size is
become
bigger by
increasing
50% in
volume and
56.8% in
mass
because
 jelly has more concentration of solute than distilled water; jelly is
hypertonic solution to pure water. Therefore, water from outside jelly will
come inside jelly as water move from high to low concentration. For jelly in
the salt water, the size is become shrink by decreasing -44.4% in volume
and -46.2% in mass because jelly has less concentration of solute meaning
that there is more water concentration inside jelly than in the salt water.
So water will move out of the cell and jelly size become smaller in order to
make balance concentration of solute and solvent between jelly and water
outside.

2. What happened to the potato after being soaked in distilled water and salt water
for an hour? Be descriptive and detailed in your answer. Use your knowledge of
membrane transport.
- When potato soaked in distilled water for an hour, it increases in
volume by 25% and mass by 16.6%. This is because of osmosis as potato
and pure water have different concentration of solute and solvent. Potato
is hypertonic solution to distilled water, so water goes inside potato and
make potato increase in size.
- Potato 2 in the salt water become smaller after soaked in the salt
water. Due to the hypertonic solution of salt water (have more solute than
solvent), the liquid inside potato goes out to salt water for making
equilibrium of between potato and water outside.
Conclusion:
From this experiment, we have observed how an objects size, shape, mass, and
volume can be affected by the process of osmosis. The potatoes and the jellies
inside the hypotonic solutions (distilled water) have an increase in both volume
and mass. Having a +50% and +56.8% change in volume and mass for the jelly,
and a +25% and +16.6 % change for the potato respectively. Whereas, the
potatoes and the jellies inside the hypertonic solutions (saltwater) have a
decrease in both its mass and volume. Having a -44.4% and -46.2% change in
the second jellys volume and mass, and a -25% and -17.9% change for the
second potato respectively.

Work log:

Name Responsibility

Genie data table

Fay Making data table, helping and

observing during the process in the
lab, organizing the lab report

Ing Purpose, Conclusion

Pond I help fill in data table

Chino Hypothesis, data table, analysis

 question