Neil Brian Yao

3A- Biochem

Names and origin

Recommended name:
Protein names     RuvB-like helicase 2
    EC=3.6.1.-
Name:  RVB2
Gene names
ORF Names:  UM04226
Organism Ustilago maydis (Smut fungus) [Complete proteome]
Taxonomic
5270 [NCBI]
identifier
Eukaryota › Fungi › Dikarya › Basidiomycota ›
Taxonomic
Ustilaginomycotina › Ustilaginomycetes › Ustilaginales ›
lineage
Ustilaginaceae › Ustilago

Protein attributes
Sequence length 476 AA.
Sequence status Complete.
Sequence processing The displayed sequence is not processed.
Protein existence Inferred from homology.

General annotation (Comments)

DNA helicase which participates in several chromatin remodeling

complexes, including the SWR1 and the INO80 complexes. The
SWR1 complex mediates the ATP-dependent exchange of
Function histone H2A for the H2A variant HZT1 leading to transcriptional
regulation of selected genes by chromatin remodeling. The
INO80 complex remodels chromatin by shifting nucleosomes
and is involved in DNA repair. Also involved in pre-rRNA
processing By similarity.

Subunit May form heterododecamers with RVB1. Component of the

structure SWR1 chromatin remodeling complex, the INO80 chromatin
remodeling complex, and of the R2TP complex By similarity.
Subcellular
location Nucleus By similarity.
Sequence
similarities Belongs to the ruvB family.
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Ontologies
Keywords
DNA damage
DNA repair
   Biological process Transcription
Transcription regulation
rRNA processing
   Cellular
Nucleus
component
ATP-binding
   Ligand
Nucleotide-binding
Activator
Chromatin regulator
   Molecular function
Helicase
Hydrolase
   Technical term Complete proteome
Gene Ontology (GO)
DNA repair
Inferred from electronic annotation. Source: UniProtKB-
KW

chromatin modification
Inferred from electronic annotation. Source: UniProtKB-
KW

rRNA processing
Inferred from electronic annotation. Source: UniProtKB-
   Biological process
KW

regulation of transcription, DNA-dependent

Inferred from electronic annotation. Source: UniProtKB-
KW

transcription

Inferred from electronic annotation. Source: UniProtKB-

KW
nucleus
   Cellular
component Inferred from electronic annotation. Source: UniProtKB-
SubCell
   Molecular function ATP binding
 Inferred from electronic annotation. Source: UniProtKB-
KW

DNA helicase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...
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Sequence annotation (Features)

Feature Graphical
Position(s) Length Description Feature identifier
key view
Molecule processing
RuvB-like
Chain 1 – 476 476 PRO_0000165673
helicase 2
Regions
Nucleotide ATP By
72 – 80 9
binding similarity
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Sequences

Mass
Sequence Length Tools
(Da)
Q4P6N7-1 [UniParc].

Last modified July 19,

FASTA 476 52,411 go
2005. Version 1.
Checksum:
F49C6B0318EF1F08
10 20 30 40 50 60
MAQISTTSEH TIQTLERIGA HSHVKGLGLD DQLEPRPSSQ GMVGQRAARK
AAGLIVKMVQ

70 80 90 100 110 120

DGRIAGRAIL MVGPPSTGKT AIAMGMAQTL GSDVPFTMLS ASEVFSLEMS
KTEALMQAFR

130 140 150 160 170 180

RSIGVRIREE AEVVEGEVVE IQIDRSLTGA TKTGKLTIKT TDMETIYELG
NKMIDSLQKE

190 200 210 220 230 240

 KVTAGDVIAI DKASGRITKL GRSFTRARDY DAMGSDTKFV QCPEGELQRR
KDVVHTVSLH

250 260 270 280 290 300

EIDVINSRTQ GFLALFSGDT GEIKPELRDQ INIKVGEWRE EGKAEIVPGV
LFIDEVHMLD

310 320 330 340 350 360

IECFSFLNRA LESELAPLVI MASNRGICRI RGTRFRSPHG IPIDLLDRVL
IISTKPYELA

370 380 390 400 410 420

DLKQILTIRA AEEEVSLKPE ALEVLTRMAS ETSLRYAINL ITTANLAAKR
RKADEVEVAD

430 440 450 460 470

VRRVYNLFVD EKRSVQYLKE HAEQFMNESD EYGDIDGLNS GAPIIGRQAS
AGAVQA

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References

"Insights from the genome of the biotrophic fungal plant

pathogen Ustilago maydis."
Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten
[1] H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K.,
McCann M., Perez-Martin J. Birren B.W.
Nature 444:97-101(2006) [PubMed: 17080091] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 521.

ProtScale
RUVB2_USTMA (Q4P6N7)

RuvB-like helicase 2 (EC 3.6.1.-)

Ustilago maydis (Smut fungus).
The parameters have been computed for the following feature:

FT CHAIN 1 476 RuvB-like helicase 2.

The computation has been carried out on the complete sequence (476 amino
acids).

SEQUENCE LENGTH: 476

Using the scale Hphob. / Kyte & Doolittle, the individual values for the 20
amino acids are:

Ala: 1.800 Arg: -4.500 Asn: -3.500 Asp: -3.500 Cys: 2.500 Gln: -3.500
Glu: -3.500 Gly: -0.400 His: -3.200 Ile: 4.500 Leu: 3.800 Lys: -3.900
Met: 1.900 Phe: 2.800 Pro: -1.600 Ser: -0.800 Thr: -0.700 Trp: -0.900
Tyr: -1.300 Val: 4.200 : -3.500 : -3.500 : -0.490

Weights for window positions 1,..,9, using linear weight variation model:

1 2 3 4 5 6 7 8 9
1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
edge center edge

MIN: -2.611
MAX: 2.156
ProtScale graph