Biopanning

Biopanning is an anity selection technique which se- 3245166 . PMID 22053087.

lects for peptides that bind to a given target.[1] All peptide
[4] Mandecki W, Chen YC, and Grihalde N. A Mathematical
sequences obtained from biopanning using combinatorial
Model for Biopanning (Anity Selection) Using Peptide Li-
peptide libraries have been stored in a special freely avail-
braries on Filamentous Phage. Journal of theoretical biol-
able database named BDB.[2][3] This technique is often ogy. 1995. 176:523-530
used for the selection of antibodies too.
[5] Smith GP, and Scott JK. Libraries of peptides and proteins
Biopanning involves 4 major steps for peptide
displayed on lamentous phage. Methods in enzymology.
selection.[4] The rst step is to have phage display 1993. 217:228-257
libraries prepared. This involves inserting foreign
desired gene segments into a region of the bacteriophage
genome, so that the peptide product will be displayed
on the surface of the bacteriophage virion. The most
often used are genes pIII or pVIII of bacteriophage
M13.[5] The next step is the capturing step. It involves
conjugating the phage library to the desired target. This
procedure is termed panning. It utilizes the binding
interactions so that only specic peptides presented by
bacteriophage are bound to the target. For example,
selecting antibody presented by bacteriophage with
coated antigen in microtiter plates.
The washing step comes after the capturing step to wash
away the unbound phages from solid surface. Only the
bound phages with strong anity are kept. The nal
step involves the elution step where the bound phages are
eluted through changing of pH or other environment con-
ditions.
The end result is the peptides produced by bacteriophage
are specic. The resulting lamentous phages can infect
Gram negative bacteria once again to produce phage li-
braries. The cycle can occur many times resulting with
strong anity binding peptides to the target.

1 References
[1] Ehrlich GK, Berthold W, and Bailon P. Phage display
technology. Anity selection by biopanning. Methods in
molecular biology. 2000. 147:195-208

[2] He, Bifang; Chai, Guoshi; Duan, Yaocong; Yan,

Zhiqiang; Qiu, Liuyang; Zhang, Huixiong; Liu, Zechun;
He, Qiang; Han, Ke (2016-01-04). BDB: biopan-
ning data bank. Nucleic Acids Research. 44 (D1):
D11271132. doi:10.1093/nar/gkv1100. ISSN 1362-
4962. PMC 4702802 . PMID 26503249.

[3] Huang, J; Ru, B; Zhu, P; Nie, F; Yang, J; Wang, X; Dai, P;

Lin, H; Guo, FB; Rao, N (Nov 3, 2011). MimoDB 2.0:
a mimotope database and beyond.. Nucleic Acids Re-
search. 40 (1): D2717. doi:10.1093/nar/gkr922. PMC

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