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DAFTAR ISI

KATA PENGANTAR................................................................................................
DAFTAR ISI.............................................................................................................
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BAB I PENDAHULUAN.........................................................................................
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BAB II TINJAUAN PUSTAKA..............................................................................
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2.1 Salak
.............................................................................................................................
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2.1.1. Syarat pertumbuhan ..................................................................................


2.1.1.1. Iklim...........................................................................................
2.1.1.2. Tanah..........................................................................................
2.1.1.3. Ketinggian Tempat.....................................................................
2.1.2 Klasifikasi salak (salacca edulis)................................................................
2.1.3. Jenis-Jenis Salak (Sallacca Edulis)............................................................
2.1.4. Khasiat Kulit Salak..................................................................................10

2.2 Kandungan Kimia Dalam Kulit Salak...............................................................10

2.3 Diabetes Mellitus...............................................................................................11

2.3.1 Jenis-jenis diabetes mellitus......................................................................11

2.3.1.1 Diabetes mellitus tipe 1..............................................................11

2.3.1.2 Diabetes mellitus 2....................................................................12

2.3.1.3 Diabetes mellitus gestasional.....................................................13

2.3.2 Diagnosis diabetes mellitus......................................................................16

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2.3.3. Gejala Diabetes........................................................................................16

2.3.4. Faktor/Penyebab Diabetes.......................................................................17

2.3.5. Bahaya Diabetes......................................................................................18

2.3.6. Cara Mengatasi Diabetes.........................................................................19

2.3.7 Penatalaksanaan diabetes mellitus............................................................20

2.3.7.1 Terapi non farmakologi..............................................................20

2.3.7.2 Terapi farmakologi.....................................................................21

2.4 Metode Pemisahan.............................................................................................25

2.5 Penapisan fitokimia...........................................................................................27

2.6 Tinjauan Umum Enzim.....................................................................................30

2.6.1 Enzim -amilase..........................................................................................34


2.6.2 Enzim -glukosidase...................................................................................36
2.7 Spektrofotometri.................................................................................................38
2.7.1Spektrofotometer UV-Vis..........................................................................38
2.7.2 Microplate Reader....................................................................................39

BAB III METODOLOGI PENELITIAN

3.1 Waktu dan Tempat Penelitian..........................................................................41

3.2 Metodologi Penelitian.....................................................................................41

3.2.1 Alat........................................................................................................41

3.2.2 Bahan....................................................................................................41

3.2.3 Rancangan penelitian............................................................................42

3.3 Prosedur Penelitian.........................................................................................42

3.3.1 Identifikasi Sampel Secara Botani........................................................42

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3.3.2 Persiapan Sampel segar dan Sampel Kering.........................................42

3.3.3 Uji Fitokimia.........................................................................................43

3.3.4 Identifikasi Alkaloid..............................................................................43

3.3.5 Identifikasi Flavonoid, Saponin, Terpenoid dan Steroid.......................43

3.4 Ekstraksi Sampel dengan Metode Infusa dan Maserasi..................................45

3.5 Uji Inhibisi Sampel Infusa dan Ekstrak Etanol terhadap Aktivitas Enzim -
amilase..................................................................................................................45

3.5.1 Penyiapan larutan..................................................................................45


3.5.2 Metode Uji Inhibisi Terhadap Enzim -amilase..................................46

3.6 Uji Inhibisi Sampel Infusa dan Ekstrak Etanol terhadap Aktivitas Enzim -
glukosidase............................................................................................................49

3.6.1 Penyiapan larutan..................................................................................49

3.6.2 Metode Uji Inhibisi Terhadap Enzim -glukosidase............................50

3.7 Analisa Data....................................................................................................54

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