CD 3200 Service Manual PDF

1 - Introduction
2 Troubleshooting
3 - Parts Lists
4 - Removal & Replacement
5 - Verification Procedures
CELL-DYN 3200 SYSTEM

SERVICE MANUAL
Part Number 9140300
4#0#,QWURGXFWLRQ Proprietary Information
4#0#,QWURGXFWLRQ
Table of Contents 1.1 Proprietary Information
This CELL- DYN 3200 System Service Manual was developed
1.1 Proprietary Information . . . . . . . . . . . . . . . . . . . . . . . . . 1
solely for use with Abbott Laboratories equipment as specified
1.2 Revision Log. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 in the operating instructions. The Manual is copyrighted by
1.3 About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Abbott Laboratories. No part of this document may be
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 reproduced, stored, or transmitted in any form or by any means
Service Manual Section Descriptions . . . . . . . . . . . . . 3 electronic, mechanical, photocopied, recorded, or otherwise
Section 1: Introduction & General Data . . . . . . . . . . . . 3 without the prior written permission of Abbott Laboratories.
Section 2: Troubleshooting . . . . . . . . . . . . . . . . . . . . . . 3 This CELL- DYN 3200 System Service Manual is published by
Section 3: Parts Lists . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Abbott Diagnostics, a Division of Abbott Laboratories, Abbott
Section 4: Removal & Replacement . . . . . . . . . . . . . . . 3 Park, IL 60064-3500, U. S. A. Please direct all inquiries
Section 5: Verification Procedures . . . . . . . . . . . . . . . . 3 concerning information in this manual to the foregoing address.
Section 6: Preventive Maintenance & Total Call. . . . . . 3 CELL- DYN is a registered trademark of Abbott Laboratories.
1.4 CELL-DYN 3200 Operators Manual . . . . . . . . . . . . . . 3
Vacutainer is a registered trademark of Becton-Dickinson and
1.5 Warranty Information. . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Company.
1.6 System Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
The CELL-DYN 3200 Hematology System . . . . . . . . . . 4 This service manual was developed to be used by trained
System External Components . . . . . . . . . . . . . . . . . . . 4 Abbott Laboratories Field Service Personnel. The revision
status of this manual is the responsibility of the manual holder.
System Internal Components . . . . . . . . . . . . . . . . . . . . 5
1.7 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 In no event shall Abbott Laboratories or its subsidiaries be
Signal Words and Graphic Warning Conventions . . . 5 liable for any damages incurred in connection with or arising
Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 from the use of this manual by persons not fully trained by
Abbott Laboratories.
Graphic Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Biosafety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 1998 by Abbott Laboratories. All rights reserved.
Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Laser Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.8 CELL-DYN 3200 Hematology Parameters . . . . . . . . . . 9
1.9 System Specifications . . . . . . . . . . . . . . . . . . . . . . . . . 10
CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 1-1
4#0#,QWURGXFWLRQ 1.2 Revision Log
1.2 Revision Log

The Revision Status of this manual is indicated below. Be sure
that the manual contains the latest revision number of all
pages.
Table 1-1 Revision Log
Revision Date Pages Revised/Added TSBs Incorporated ISAs Incorporated

A August Complete Revision 109 - 001A, 003 109 - 003, 004, 005, 006
4#0#,QWURGXFWLRQ 1.3 About This Manual
1.3 About This Manual Section 4: Removal & Replacement

This section contains the procedures to be used by the FSR for
Introduction the removal and replacement of subassemblies and
components. The procedures are presented in a paragraph
This Service Manual contains the information necessary to format and are accompanied by illustrations for added clarity.
align, troubleshoot, repair, and maintain the CELL-DYN 3200
Automated Hematology System. Section 5: Verification Procedures
This section contains procedures that enable the FSR to verify
Service Manual Section Descriptions the integrity of various instrument functions. These procedures
The manual is composed of five sections. The following are may be used to facilitate troubleshooting, or be dictated by
descriptions of the contents of each of the sections. Troubleshooting and Removal and Replacement.
This section also contains procedures such as adjustments,
Section 1: Introduction alignments, and calibrations used to ensure proper instrument
This section contains an introduction to the system, information performance. These procedures may be performed on a routine
on the use of the manual, warranty information, safety maintenance basis, or be dictated by Removal and
considerations, hematology parameters, and system Replacement.
specifications.
1.4 CELL-DYN 3200 Operators Manual
Section 2: Troubleshooting Operating instructions and routine maintenance procedures for
This section contains procedures to be used by the FSR in the CELL-DYN 3200 are included in the Operators Manual
identifying and isolating problems. The procedures are provided with each system. It is essential that the service
presented in a flowchart format and are accompanied by engineer read and understand the Operators Manual, and be
illustrations for added clarity. A detailed explanation of the use able to correctly perform all routine operating functions before
of the flowcharts is given at the beginning of the section. attempting to troubleshoot and repair the system.
Section 3: Parts Lists

This section contains parts lists and graphics showing the
locations of FRUs. This section is used by the FSR in
conjunction with Troubleshooting and Removal & Replacement.
4#0#,QWURGXFWLRQ 1.5 Warranty Information
1.5 Warranty Information System External Components

Components comprising the CELL-DYN 3200 System have The following is a list of the CELL-DYN 3200 external
been designed by Abbott Laboratories for optimal performance. components and conditions:
Substitution of reagents, accessories, or system components
may adversely affect performance and may invalidate any Operator Technique (Maintenance)
warranty agreements. System Reagents
Abbott Laboratories cannot accept responsibility for any clinical Isotonic Diluent
results produced by use of reagents, calibrators, controls, Diluent/Sheath
equipment, or supplies either not manufactured by or not Hemoglobin Lyse
approved by Abbott Laboratories. WBC Lyse
System Controls & Calibrators
1.6 System Introduction CELL-DYN 3200 Whole Blood Control
Each CELL-DYN 3200 System may be comprised of three or OPTI-CAL
four major hardware components: Analyzer, Video Display, Latex Beads
Keyboard, and optional Sample Loader. Whole Blood Specimens
System Environmental Integrity (Temperature, RFI, ESD)
The CELL-DYN 3200 Hematology System
Power Line Integrity
When approaching a hematology system from a service
perspective, the word system must be emphasized because
there are both internal components (system hardware and
software) and external components (reagents, controls, etc.)
that comprise the complete system. The Service
Representative must realize that both of these types of
components can adversely affect overall system performance.
Therefore, it follows that all external components and
conditions such as reagents, environment, integrity of samples
and controls be checked and verified as correct before
performing service on the internal components. In the
investigation of any non-specific or data related complaint, the
system hardware and software should be the last components
of the system to be suspected as the cause of the problem.
4#0#,QWURGXFWLRQ 1.7 Safety
System Internal Components Signal Words and Graphic Warning

The following is a list of the CELL-DYN 3200 internal Conventions
components:
Signal Words
CELL-DYN 3200 System Components
'$1*(5=##Denotes an immediate hazard which, if not
Flow Panel
avoided, could result in serious injury or death. This signal word
Optics Bench represents the highest level of any hazardous situation.
Power Supplies
Measurement Electronics :$51,1*=##Denotes a hazard which, if not avoided, could
Computer Electronics result in moderate to serious injury.
A/D & D/A Electronics &$87,21=##Denotes potential hazards that could result in
Air Supply minor injury. Also, used for conditions or activities that could
Personal Computer Module threaten equipment performance.
Tube Manipulation Hardware
Graphic Conventions
1.7 Safety The general hazard icon identifies an activity or area
Operation, maintenance, and servicing of hematology systems that may present a hazard to personnel or equipment.
may expose individuals to potential safety and health hazards.
All work must be performed in accordance with procedures
described in the Abbott Operators and Service Manuals, or as
directed by an Abbott Technical Support Representative.
The electrical hazard icon alerts personnel to the
Warnings are inserted in this manual to alert personnel to possibility of electrical shock if procedural or
potential hazards. The standard warning conventions including engineering controls are not observed.
signal words (e.g. Caution) and symbols are described below.
Clean-up spills of potentially infectious materials in accordance

The biohazard icon identifies an activity or area where with established biosafety practices. A generally accepted
personnel may be exposed to infectious substances. procedure for cleaning such spills is to absorb the spill with
toweling or other absorbent material, wipe the area with a
detergent solution, and then wipe the area with an appropriate
tuberculocidal disinfectant such as 10% chlorine bleach
The laser hazard icon identifies an activity or area solution.
where personnel will be exposed to an eye hazard if Prior to service or maintenance, the instrument should be
procedural or engineering controls are not observed. decontaminated in accordance with the following:
a. Remove and dispose of contaminated disposables in a
regulated medical waste container.
The electrostatic discharge icon identifies an activity or b. Clean and decontaminate the exterior of the
area where personnel must wear a ground strap while instrument using a detergent solution followed by a
servicing the system. 10% chlorine bleach solution or other tuberculocidal
disinfectant. Flush the fluid pathway with chlorine
bleach solution as specified in the Operators Manual.
Biosafety
Dispose of waste in accordance with local, state and federal
:$51,1*=#327(17,$/#%,2+$=$5' Consider regulations. Probes, needles, broken glass, slides, and other
all clinical specimens, reagent controls, surfaces, or sharps that are contaminated with potentially infectious
components that contain or have contacted human substances should be collected in a sharps container for
blood or serum as potentially infectious. Wear gloves, disposal as regulated medical waste. Contaminated gloves,
lab coats and safety glasses, and follow other wipes, and other disposables should be placed in a standard
biosafety practices as specified in the OSHA medical waste container.
Bloodborne Pathogen Rule (29CFR Part 1910.1030)
or other equivalent biosafety procedures.
:$51,1*=#327(17,$/#%,2+$=$5'#The
aspiration needle/probe is sharp and potentially
contaminated with infectious materials. Avoid contact
with the tip of the needle/probe.
Electrical Safety The Class 1 Laser Product Label (Abbott PN 9230702A) is

shown below (Figure 1-1). The label consists of black lettering
:$51,1*= (/(&75,&$/#+$=$5' When the against a white background. The label is located on the
instrument is powered and protective covers are backside of the instrument and positioned at a clearly visible
removed, there are exposed electrical systems that location.
could startle or seriously injure personnel upon The inner protective cover laser warning labels must not be
contact. Use appropriate safety precautions to prevent removed and are to remain legible. The protective housing
body and/or tool contact with live electrical label (Abbott PN 9230701D) is shown in Figure 1-2. The label
components, especially power supplies. consists of black lettering against a yellow background and
appears on the protective cover within the optics bench and on
Laser Safety the flow panel (see Figure 1-3 and Figure 1-4).
DANGER: LASER LIGHT Avoid direct exposure to

beam. Do not look directly into the laser beam or any
reflection of the beam from a mirror-like surface.
When the access door, instrument top cover, or other
inner protective cover is removed, (Helium-Neon)
laser power up to 10 mW continuous wave at 632.8
nm in a beam with 1 mR divergence could be
accessible in the interior of the optics bench. This
32109
amount of energy, with insignificant attenuation with
distance, is sufficient to cause eye damage. Figure 1-1 Laser Product Label (PN 9230702A)
&$87,21: Use of controls or adjustments or

performance of procedures other than those specified
herein may result in hazardous laser light exposure. If
the instrument is used or modified in a manner not
specified by the manufacturer, the protection provided
by the instrument may be impaired.
.CUGT
9CTPKPI
.CDGN
Figure 1-2 Laser Warning Label (PN9230702D)

Figure 1-3 Top Laser Warning Label Location
For beam alignment and other open beam configurations,
follow instructions provided by the Service Manual, Advisories,
and Bulletins regarding the requirement for using laser safety
eyewear. If required, assure laser eyewear are not damaged
and have an optical density of 1- 2 at a wavelength of 632.8
nm. During open beam configurations, assure that the beam is
confined to the laser bench area and only personnel with proper
eye protection are present.
4#0#,QWURGXFWLRQ 1.8 CELL-DYN 3200 Hematology Parameters
1.8 CELL-DYN 3200 Hematology

.CUGT
Parameters
9CTPKPI The following tables list the CELL-DYN 3200 Hematology
.CDGN Parameters and their abbreviations.
Table 1-2 Red Cell Parameters
Full Name Abbreviation

Red Blood Cell Concentration RBC
Hemoglobin Concentration HGB
Hematocrit HCT
Mean Cell Volume MCV
Mean Cell Hemoglobin MCH
Mean Cell Hemoglobin Concentration MCHC
Red Cell Distribution Width RDW
Table 1-3 White Cell Parameters

Figure 1-4 Front Laser Warning Label Location Full Name Abbreviation
White Blood Cell Concentration WBC
Neutrophil Concentration NEU
Neutrophil Percentage %N
Lymphocyte Concentration LYM
Lymphocyte Percentage %L
Monocyte Concentration MONO
Monocyte Percentage %M
Eosinophil Concentration EOS
4#0#,QWURGXFWLRQ 1.9 System Specifications
Table 1-3 White Cell Parameters (Continued) 1.9 System Specifications

Full Name Abbreviation The following tables contain the specification for the CELL-DYN
3200 System.
Eosinophil Percentage %E
Basophil Concentration BASO Table 1-5 Physical Specifications (Unpackaged)
Basophil Percentage %B Graphics
CS Model SL Model
Printer
Height 19.0 (48.2 cm) 19.0 (48.2 cm) 7.2 (18.3 cm)
Table 1-4 Platelet Parameters
Width 32.8 (83.3 cm) 32.8 (83.3 cm) 16.1 (40.9 cm)
Full Name Abbreviation
Depth 23.3 (59.2 cm) 29.3 (74.4 cm) 9.9 (25.1 cm)
Platelet Concentration PLT
Weight 214 lb (97.3 kg) 232 lb (105.5 kg) 9.9 lb (4.5 kg)
Mean Platelet Volume MPV
Platelet-crit PCT* Table 1-6 Physical Specifications (Packaged)
Platelet Distribution Width PDW*
Graphics
* Clinical significance has not been established for these CS Model SL Model
Printer
parameters. Therefore they are not reportable in the US.
They are provided for laboratory use only. Height 38 (96.5 cm) 38 (96.5 cm) 12 (30.5 cm)
Width 34 (86.3 cm) 34 (86.3 cm) 19.5 (49.5 cm)
Depth 31 (78.7 cm) 31 (78.7 cm) 14.8 (37.6 cm)
Weight 255 lb (116 kg) 273 lb (124 kg) 16.4 lb (7.4 kg)
CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 1 - 10
Table 1-7 Analyzer Input Requirements Table 1-8 Performance Specifications
Max Linear
Module Voltage Frequency Background Precision Correlation
Current Range
110 VAC + 10 % 50/60 3 Hz 6 amps WOC 0.10K/L 2.7% 0.99 0.0-250 K/L
120 VAC + 10 % 50/60 3 Hz 6 amps
Analyzer NOC 0.10 K/L 2.7% 0.99 0.0-250 K/L
220 VAC + 10 % 50/60 3 Hz 3 amps
RBC 0.20 M/L 1.5% 0.98 0.0-8.0 M/L
240 VAC + 10 % 50/60 3 Hz 3 amps
110 VAC + 10 % 50/60 3 Hz 6 amps HGB 0.1g/dl 1.0% 0.98 0.0-25.0g/dL
Analyzer &
120 VAC + 10 % 50/60 3 Hz 6 amps MCV N/A 1.0% 0.98 35-180 fL
Sample
Loader 220 VAC + 10 % 50/60 3 Hz 3 amps RDW N/A 5.0% 0.91 N/A
240 VAC + 10 % 50/60 3 Hz 3 amps
0.0 - 1750
PLT 5.0K/L 4.0% 0.98
Monitor 100 - 240 VAC 50/60 1.3 amps K/L
120 VAC 10%
Printer 50/60 0.5 amps MPV N/A 5.0% 0.72 2.0-18.0 fL
220 - 260 VAC
Table 1-9 Operational Specifications

Specimen Tube Dimensions
Operating Environment Diameter 13 mm
Temperature 15o - 30o C (59o - 86o F) Length 75 mm
Relative Humidity 20% to 80%, RHNC Minimum Specimen Volumes
Complete Cycle Times Closed and Sample Loader Mode 1 mL
Startup (From Power Off) ~ 7 minutes Open Mode 500 L
Startup (From Standby) ~ 5 minutes Batch Size
Count Cycle, Patient ~ 52 seconds 1 to 50 tubes per batch
Count Cycle + FrgWBC ~ 100 seconds Bar Code Formats
Count Cycle + ResRBC ~ 100 seconds Interleaved 2-of-5 code
Shutdown (To Standby) ~ 130 seconds Codabar
Shutdown (To Power Off) ~ 130 seconds Code 39
Throughput Code 128 (check digit only)
CS Model Bar Code Specifications
Open 80 Samples/Hour Printed on good quality label stock

Closed 68 Samples/Hour 0.25-inch quiet zone on each end
SL Model 0.01-inch minimum narrow bar width
Open 80 Samples/Hour 0.5-inch minimum bar length
Closed 68 Samples/Hour 2-inch maximum label length
Whole Blood Aspiration Volume 1.25-inch maximum label width
Open Mode 125 L
Closed Mode 250 L
5#7URXEOHVKRRWLQJ Table of Contents TOC
5##7URXEOHVKRRWLQJ
Table of Contents
2.1 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2 IP-25 Hard Drive Errors . . . . . . . . . . . . . . . . . . . . . . . . . 2-78

2.2 Troubleshooting Approach . . . . . . . . . . . . . 2-2 IP-26 Printer Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-79
IP-27 Sample Loader & Aspiration Tower
2.3 Error Messages . . . . . . . . . . . . . . . . . . . . . . . 2-3 Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . 2-81
IP-1 Fluid Leaks, Open Mode Probe or Wash Block . . . 2-4 Appendix 2A -Cable Connection Diagram . . . . . . . . . . . . 2A-1
IP-2 Fluid Leaks, Closed Mode Needle or Wash Block . 2-6 Appendix 2B -System Block Diagrams . . . . . . . . . . . . . . 2B-1
IP-3 Incomplete Aspiration . . . . . . . . . . . . . . . . . . . . . . . 2-8 Appendix 2C -System Flow Diagrams . . . . . . . . . . . . . . . 2C-1
IP-4 Flow Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-11
IP-5 RBC/HGB Diluent Overpressure . . . . . . . . . . . . . 2-13
IP-6 Optical Parameter Count Problems . . . . . . . . . . . 2-15
IP-7 Reagent Empty . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-26
IP-8 Optical Backgrounds Too High . . . . . . . . . . . . . . . 2-31
IP-9 Histogram Problems . . . . . . . . . . . . . . . . . . . . . . . 2-32
IP-10 Controls Out . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-33
IP-11 Vacuum Accumulator #1 Wet . . . . . . . . . . . . . . . . 2-35
IP-12 Vacuum Accumulator #2 Wet . . . . . . . . . . . . . . . . 2-37
IP-13 HGB Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-39
IP-14 X-B Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-41
IP-15 Optical Parameters Imprecision . . . . . . . . . . . . . . 2-44
IP-16 HGB Imprecision . . . . . . . . . . . . . . . . . . . . . . . . . 2-47
IP-17 Primary Vacuum/Pressure Isolation, . . . . . . . . . . 2-48
IP-18 Pressure Leak Isolation . . . . . . . . . . . . . . . . . . . . 2-57
IP-19 Vacuum Leak Isolation . . . . . . . . . . . . . . . . . . . . . 2-64
IP-20 Shear Valve Position Fault . . . . . . . . . . . . . . . . . . 2-70
IP-21 Flags . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-72
IP-22 External Waste Full . . . . . . . . . . . . . . . . . . . . . . . 2-74
IP-23 Blank Display. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-76
IP-24 HSSL Failures/Message Reception Time-Out . . . 2-77
CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300-August 1998 2-1
5#0#7URXEOHVKRRWLQJ 2.1 Summary
2.1 Summary c. To refer to the appropriate IP or VP, click on the red

link.
This chapter contains the following information for CELL-DYN d. Begin at the START box and follow the directions in the
3200: IP.
Error Messages e. If a part is adjusted or replaced, go to Section 5.2,
Verification Matrix.
Isolation Procedures (IPs)
f. Find the part in left column.
Block Diagrams
g. Perform VPs in order given (i.e., 1, 2, 3, 4 etc.)
Normal Scatter/Histograms
2. Perform Total Call.
Service Commands
2.2 Troubleshooting Approach

Troubleshooting is required whenever an Observed Error or a
Displayed Error occurs. Displayed Errors can be viewed on the
Bulletin Line or status box of the Data Station screen. (The
Bulletin Line is down about 3/4 way on the screen.) Detailed
information can be found in the fault report screen of the
diagnostics submenu.
When a malfunction occurs, follow these recommended
procedures:
1. Use this Troubleshooting Guide as follows:
a. If possible, begin by verifying the reported error.
Perform the exact procedure that produced the error
condition.
b. When an error occurs, either observed or displayed,
look up the error in:
the Error Messages table or
IP 27, Sample Loader & Aspiration Tower Error
Messages table
CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 - August 1998 2-2
5#0#7URXEOHVKRRWLQJ 2.3 Error Messages
2.3 Error Messages Error Corrective Action

Invalid Tube Height IP-27
Error Corrective Action
Leaks, Pressure IP-18
Background, HGB Too High IP-13
Leaks, Vacuum IP-19
Background, RBC/ PLT Too High IP-8
Load Zone Empty Detector
Background, WBC Too High IP-8 IP-27
Malfunction
Bar Code Reader Failure IP-27 MCH Errors IP-14
C:\ Hard Drive Errors IP-25 MCHC Errors IP-14
Controls Out IP-10 MCV Errors IP-14
Diluent/Sheath Empty IP-7 Message Reception Timeout IP-24
Erratic Results, HGB IP-13 Mix Head Not At Top Position IP-27
IP-6 Ensure Blood Sam Mix Head Stuck At Top Position IP-27
ple is Fresh. Differential
Mix Head Stuck In Angle Position IP-27
count is not reliable
Erratic Results, Optical Mix Head Stuck In Vertical
before 30 minutes or after IP-27
12 hours after sample is Position
drawn. Mix Zone Rack Position Error IP-27
External Waste Full IP-22 No Tube Present IP-27
Flags IP-21 Overpressure, Diluent Syringe IP-5
Flow Errors IP-4 Precision Problems HGB IP-16, OPT IP-15
Fluid Leaks, Closed Mode Probe IP-2 Pressure Errors IP-17
Fluid Leaks, Open Mode Probe IP-1 Printer Errors IP-26
HCT Errors IP-14 Probe Stuck At Home Position IP-27
HGB Errors IP-13 Probe Unable To Reach Home
IP-27
Histogram Errors IP-9 Position
HSSL Com Failures IP-24 Rack Bar Code Read Failure IP-27
Incomplete Aspiration IP-3 Shear Valve Position Fault IP-20
CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 - August 1998 2-3
5#0#7URXEOHVKRRWLQJ 2.3 Error Messages
Error Corrective Action

Tube Dropped During Mixing IP-27
Tube Lost Moving From Position
IP-27
3 To 4
Tube Position Bar Code Read
IP-27
Failure
Tube Stuck In Position 3 IP-27
Tube Stuck In Position 4 IP-27
Unexpected Tube In Position 4
IP-27
After Rack Advance
Unload Area Hardware
IP-27
Malfunction
Unload Zone Full Detector
IP-27
Malfunction
Unload Zone Nearly Full Detector
IP-27
Malfunction
Vacuum Accumulator 1 Wet IP-11
Vacuum Accumulator 2 Wet IP-12
Vacuum Errors IP-17
IP-15 Ensure Blood Sam-
ple is Fresh. Differential
count is not reliable
WBC Imprecision
before 30 minutes or after
12 hours after sample is
drawn.
WBC/HGB Lyse Empty IP-7
X-B Errors IP-14
CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 - August 1998 2 - 3A
5#7URXEOHVKRRWLQJ IP-1 Fluid Leaks, Open Mode Probe or Wash Block
IP-1 Fluid Leaks, Open Mode Probe or Wash Block (Page 1 of 2)
Start
- Clamp tubing at the top of

- Clamp tubing between Valve 11
Ultrasonic sensor and
YES Shear valve
NO
Probe Leaking
In Ready Mode Leaking
Stops
? ?
Leaking
Stops
YES
NO ?
- Verify pinch tubing in

YES Valve 11
- Verify and replace NO
Valve 11 (PL-4.1)
- Verify connections on
Wash Block Leaking
NO Ultrasonic Sensor
In Ready Mode
- Verify and tighten fittings
on Y-Valve
? - Verify connection on top
of Probe - Verify Shear valve
- Replace Y-Valve (PL-8) fittings
YES - Clean Shear Valve
(VP-48)
- Replace Shear Valve
1 2 (PL-9.1)
CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300-August 1998 2-4
5#7URXEOHVKRRWLQJ IP-1 Fluid Leaks, Open Mode Probe or Wash Block
IP-1 Fluid Leaks, Open Mode Probe or Wash Block (Page 2 of 2)
1 2
- Verify pinch tubing in - Run a several Count

Valve 15 Cycles while observing the
- Verify and replace Valve bottom of the Wash Block - Ensure Vacuum #1 and Pressure #3
15 (PL-4.1) are within specifications (VP-16)
- Ensure bottom fitting on Wash Block
YES is not restricted
- Ensure tubing between Wash Block
and Valve 14 is not restricted
- Verify operation of Valve 14 and
- Ensure fittings on Wash NO Liquid replace if necessary (VP-10), (PL-4.1)
block are not leaking Leaking - Replace pinch tubing in Valve 14
(TM-1)
?
- Ensure tubing between Valve 14 and
WC #4 is not restricted
- Verify operation of right Vent Trap
Check Valve and replace if necessary
(TM-1)
- Ensure tubng between WC #4 and
Valve 31 is not restricted
- Verify operation of Valve 31 and
replace if necessary (VP-10), (PL-4.1)
- Replace pinch tubing in Valve 31
(TM-1)
5#7URXEOHVKRRWLQJ IP-2 Fluid Leaks, Closed Mode Needle or Wash Block
IP-2 Fluid Leaks, Closed Mode Needle or Wash Block (Page 1 of 2)
Start
- Clamp tubing at the top of

- Clamp tubing between Valve 11
Leave tubing connected, and
Ultrasonic sensor and
remove Needle from Wash Block
Shear valve
YES NO
Leaking
Stops
Probe Leaking ?
Leaking
In Ready Mode
Stops
?
? YES
NO
- Verify pinch tubing in
YES NO
Valve 11
- Verify and replace
Valve 11 (PL-4.1)
- Verify connections on
Wash Block Leaking Ultrasonic Sensor
NO
In Ready Mode - Verify and tighten fittings
on Y-Valve - Verify Shear valve
- Verify connection on top fittings
?
of Probe - Clean Shear Valve
- Replace Y-Valve (PL-8) (VP-48)
YES - Replace Shear Valve
(PL-9.1)
1 2
5#7URXEOHVKRRWLQJ IP-2 Fluid Leaks, Closed Mode Needle or Wash Block
IP-2 Fluid Leaks, Closed Mode Needle or Wash Block (Page 2 of 2)
1 2
- Verify pinch tubing in - Run a several Count

Valve 34 Cycles while observing the
- Verify and replace Valve bottom of the Wash Block
34 (PL-4.1)
- Ensure Vacuum #1 and Pressure #3 are
within specifications (VP-16)
- Ensure bottom fitting on Wash Block is not
YES
restricted
- Ensure tubing between Wash Block and
- Ensure fittings on Wash NO Liquid Valve 37 is not restricted
block are not leaking Leaking - Verify operation of Valve 37 and replace if
necessary (VP-10), (PL-4.1)
? - Replace pinch tubing in Valve 37 (TM-1)
- Ensure tubing between Valve 37 and WC #4
is not restricted
- Verify operation of right Vent Trap Check
Valve and replace if necessary (TM-1)
- Ensure tubng between WC #4 and Valve 31 is
not restricted
- Verify operation of Valve 31 and replace if
necessary (VP-10), (PL-4.1)
- Replace pinch tubing in Valve 31 (TM-1)
5#7URXEOHVKRRWLQJ IP-3 Incomplete Aspiration
IP-3 Incomplete Aspiration (Page 1 of 3)
Start
- Verify Y-Valve is rotating - Aspirate Normal

properly (VP-7) . YES
NO Control in both open
- Clean Shear Valve (VP-48) . and closed modes.
Rotating
Properly
Mode(s)
? Affected
Problem Open Only ? Both
Corrected
NO
?
Closed Only
YES - Replace Y-Valve Assy (PL-8) .

1 2 4
- Replace MDM (Pl-9.2)
Total Call - Replace SDM/CDM (PL-15) .
1 2
- Manually place a blood

- Run a closed cycle and sample under Closed
- Run an open cycle and - Place a blood sample Probe.
time the blood
time the blood movement under Open Probe and
movement to the - From Main Menu, press
to the Ultrasonic Sensor. manually open Valve 12
Ultrasonic Sensor. Start DIAGNOSTICS
Start when Valve 12 until the blood is just
when Valve 12 opens YES
opens and stop when past the Y-Valve. MORE
and stop when blood
blood reaches sensor. YES - Verify an air gap of reaches sensor. TOWER TEST
approximately 1 inch NEEDLE DOWN
between the diluent and
blood columns. - Manually open Valve 12
until the blood is just
- If no air gap is present, past the Y-Valve.
check the Open Probe < 7.0 Seconds
plumbing for air leaks. - Verify an air gap of
< 3.5 Seconds ? approximately 3 inches
between the diluent and
? NO blood columns.
NO
- Clean Closed Probe (VP-51).

- Clean Open Probe (VP-50). - Ensure there are no
restrictions between Closed
- Ensure there are no Air Gap
Probe and Y-Valve. NO
restrictions between Open Present
Probe and Y-Valve. - Replace Closed Probe (PL-8).
?
- Replace Open Probe (PL-8). - Verify Valve 38 (VP-10) and
ensure Vent Trap plumbing is
free of restrictions. YES
- Check the Closed
Probe plumbing for air
leaks. 3
3 4
- Run an open cycle and

- INITIALIZE and PRIME - Run an open cycle and
time the blood
instrument. verify that blood column
movement to the
- Run a closed cycle and Ultrasonic Sensor. Start stops just past
verify that the blood when Valve 12 opens Ultrasonic Sensor. I
column stops just past and stop when blood blood does not stop:
Sample Sensor #2. reaches sensor. YES - Re-seat Ultrasonic
- If blood does not stop: Sensor Cable.
- Perform Sample Sensor - Replace Ultrasonic
adjustment (VP-12). Sensor (PL-8).
- Re-seat sensor cable. - Replace Ultrasonic
- Replace Sample Sensor Board (PL-9.2).
< 3.5 Seconds
Sensor (PL-9.1).
- Replace Sample ?
Sensor Board (PL-9.2).
NO
- Verify Vac 2 level (VP-16). Troubleshoot as necessary.

- Ensure there are no restrictions between Y-Valve and
Valve 12
- Verify Valve 12 and tubing (VP-10). Replace as necessary
(PL-4.1), (TM-1).
5#7URXEOHVKRRWLQJ IP-4 Flow Errors
IP-4 Flow Errors (Page 1 of 2)
Sample
Staging
Pump
Start
From Main Menu:

DIAGNOSTICS
YES
MORE
- Run a Count Cycle and observe MOTOR OPERATION
the Delivery Syringe and WC1. EXERCISE MOTORS WC1
Verify that when the syringe is
- Type A and press Enter.
moving up, liquid is dripping into
WC1 at a fast and constant rate - Observe the Delivery Syringe
(See Figures). and verify smooth upward
movement (See Figures). 68
Delivery Syringe
Dripping
OK Smooth
Movement
?
? YES
NO
NO
1 2
3
5#7URXEOHVKRRWLQJ IP-4 Flow Errors
IP-4 Flow Errors (Page 2 of 2)
1 2 3
- Remove and clean Delivery

Syringe.
- Check Laser for stable
- Verify level of 9 psi (VP-16) - Replace Delivery Syringe
output (VP-46).
- Verify tubing and operation of (PL-7).
Valves 64, 65, 91, and 56 and - Replace Syringe Driver
replace as necessary (TM-1), Assembly (PL-7).
(PL-4.1). - Replace MPM (PL-11).
- Perform Optical Flow Cell
Cleaning Procedure (VP-44).
- Replace In-Line Sheath Filter Output
YES
(PL-4.2). Stable
?
- Remove, inspect, and clean

NO Flow Cell Nozzle.
- Replace Flow Cell Assy. (PL-10).
- Replace Laser (PL-10).

- Replace Laser Power Supply
(PL-2.2).
5#7URXEOHVKRRWLQJ IP-5 RBC/HGB Diluent Overpressure
IP-5 RBC/HGB Diluent Overpressure (Page 1 of 2)
Start
- Verify Shear Valve rotation time

- Check resistance of is <1.1 sec
- Remove, inspect, and clean
Overpressure Sensor Shear Valve (VP-48) From Main Menu:
- Ensure diluent paths from DIAGNOSTICS
NO syringe to RBC and HGB MORE
Mixing Chambers are free of MOTOR OPERATION
restrictions SHEAR VAL DISPENSE
Sensor - Verify operation of Valves 27 SHEAR VAL TIME
NO
Shorted and 26 (VP-10) and replace if
necessary (PL-4.1) SHEAR VAL ASPIRATE
? SHEAR VAL TIME
YES
- Replace Sensor (PL-4.2)

Times
Problem OK
Corrected YES ?
?
Total Call
NO
YES
1 2
Total Call
5#7URXEOHVKRRWLQJ IP-5 RBC/HGB Diluent Overpressure
IP-5 RBC/HGB Diluent Overpressure (Page 2 of 2)
1 2
In Ready Mode, try to manually

rotate the front and back of the - Lubricate Shear Valve Driver
Shear Valve Sections (VP-42)
Shear Valve. Should Not Move
If problem persists:
- Replace Shear Valve Driver
If there is movement: (PL-9.1)
- Replace defective section:

Front (PL-9.1)
Rear (PL-9.1) Rim Notch
If problem persists: Faces Right
- Replace Shear Valve Driver Total Call
(PL-9.1) 32002
Total Call
5#7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems
IP-6 Optical Parameter Count Problems (Page 1 of 11)
Note: This procedure assumes that HGB Results are OK in Open and Closed Modes, and there are no hardware or software Error Messages.
It is designed to find and correct gross WBC, NOC, RBC, and PLT problems. For Optical Precision Problems see IP-15.
Start
- Verify proper Reagents and Expiration All "0" YES 3 RBC Only "0" YES 6
Date.
? ?
- Perform Background Count Verification
(VP-56).
NO NO
Go to (IP-8),
Backgrounds
NO correct and
RBC & PLT PLT Only "0" YES 7
YES 4
OK Only "0"
return here.
? ? ?
YES
NO
NO
NO
From Main Menu, press
RUN
SPECIMEN TYPE NOC Only "0" YES 8
WBC Only "0" YES 5
FRAGILE WBC ?
- Run a Normal Control five times and ?
record the optical values. NO
- Compare the results to the Data Sheet.
1
NOC Only WBC Only

YES 12 YES 15
Low High
? ?
All Low YES 9
? NO NO
NO
NOC Only
All High YES 13 YES 16
High
RBC & PLT ? ?
YES 10
Only Low
? NO 2
NO
NO
NO
RBC & PLT

YES 14
Only High
WBC Only
YES 11 ?
Low
?
WBC Only
All Erratic YES 17 YES 19
Erratic
? ?
NO NO
RBC & PLT NO NOC Only

YES 18 YES 20
Only Erratic Erratic
? ?
NO
Total Call
3
- Verify beam alignment
(VP-20), (VP-21).
- Verify laser power (VP-46).
- If not able to get data on
- Verify samples are being staged to the Extended Count
Optical Flow Cell by Peri-pump. YES
Screen, replace in order
Troubleshoot as necessary. the Optical Flow Cell,
- Verify Sheath Reservoir is filling MAM, SPM, and CPU/
properly. D C M (PL-10), (PL-12),
Power OK (PL-13).
- Verify Pressure 2 (VP-16) and
proper Sheath flow to Optical Flow ?
Cell. Troubleshoot as necessary.
NO
- Replace In-Line Filter (PL-4.2). NO
- Verify operation of Sample
Injection Syringe (VP-6), (VP-7).
Troubleshoot as necessary. - Connect new Laser Head to
Laser Power Supply and
verify power (see Warning).
NO
If good, replace Laser Head
(PL-10). If not, replace Laser
Power Supply (PL-2).
Problem
Corrected
? 'DQJHU=#+LJK#9ROWDJH#00#$VVXUH#WKH#SRZHU#WR#WKH#LQVWUXPHQW#LV#
'DQJHU
RII1#$#KLJK#YROWDJH#FKDUJH#PD\#UHPDLQ#RQ#WKH#ODVHU#FRQQHFWRU#
ZLWK#WKH#SRZHU#RII1#'R#QRW#WRXFK#WKH#PDOH#SLQV1#6KRUW#PDOH#SLQV#
YES
WR#&KDVVLV1
Total Call
4 5 6 7
- Replace MAM (PL-12). - Replace MAM (PL-12).

- Verify RBC/PLT Sample is being - Verify WBC Sample is being - Replace SPM (PL-10). - Replace SPM (PL-10).
staged to Optical Flow Cell. staged to Optical Flow Cell.
- Replace CPU/DCM (PL-13). - Replace CPU/DCM (PL-13).
- Verify operation of Valve 54 (VP- - Verify operation of Valve 53
10) and tubing. Replace as and 55 (VP-10) and tubing.
necessary (PL-4.1), (TM-1). Replace as necessary (PL-
4.1), (TM-1). 8
- From Main Menu, press
DIAGNOSTICS - Verify operation of WBC
Syringe (VP-6), (VP-7).
F12 - Verify NOC Sample is
F1 being staged to Optical
DIAGNOSTICS Flow Cell.
SET POINT ENTRY
F12 - Verify operation of Valve
- Verify RBC/PLT Threshold is
550. F1 41 (VP-10) and tubing.
- Reseat Preamp, MAM, SPM, SET POINT ENTRY Replace as necessary
- Verify WBC Threshold is (PL-4.1), (TM-1).
CPU/DCM Cables.
360. - From Main Menu, press
- Replace MAM (PL-12).
- Reseat Preamp, MAM, SPM, DIAGNOSTICS
- Replace 10 o Preamp (PL-10).
CPU/DCM Cables. F12
- Replace SPM (PL-10).
- Replace MAM (PL-12). F1
- Replace CPU/DCM (PL-13). o
- Replace 0 Preamp (PL-10). SET POINT ENTRY
- Replace SPM (PL-10). - Verify WBC Threshold is
- Replace CPU/DCM (PL-13). 400.
- Perform NOC Gain
Adjustment (VP-25).
- Verify laser power (VP-46).

- Clean Laser Bench (VP-43).
- Clean, debubble, and wet
-- Verify Sheath Reservoir is filling
YES Optical Flow Cell (VP-44),
properly.
(VP-45).
- Verify Pressure 2 (VP-16) and
- Verify beam alignment (VP-
proper Sheath flow to Optical Flow
Power OK 20), (VP-21).
Cell. Troubleshoot as necessary.
- Replace Optical Flow Cell (PL-
- Replace In-Line Filter (PL-4.2). ? 10).
- Verify operation of Sample
NO - Replace MAM (PL-12).
Injection Syringe (VP-6), (VP-7).
NO - Replace CPU/DCM (PL-13).
Troubleshoot as necessary.
- Replace Vent Accumulator Filter
(PL-4.2). - Connect new Laser Head to
Laser Power Supply and
verify power (See Warning).
NO If good, replace Laser Head
(PL-10). If not, replace
Laser Power Supply (PL-2).
Problem
Corrected
'DQJHU=#+LJK#9ROWDJH#00#$VVXUH#WKH#SRZHU#WR#WKH#LQVWUXPHQW#
'DQJHU
?
LV#RII1#$#KLJK#YROWDJH#FKDUJH#PD\#UHPDLQ#RQ#WKH#ODVHU#
FRQQHFWRU#ZLWK#WKH#SRZHU#RII1#'R#QRW#WRXFK#WKH#PDOH#SLQV1#
YES 6KRUW#PDOH#SLQV#WR#&KDVVLV1
Total Call
10 11
- Verify RBC/PLT Sample is - From Main Menu, press - From Main Menu, press
being staged to Optical DIAGNOSTICS - Verify WBC Sample is DIAGNOSTICS
Flow Cell. being staged to Optical
F12 F12
- Verify operation of Valve 54 Flow Cell.
(VP-10) and tubing. F1 F1
- Verify operation of Valve 53
Replace as necessary (PL- SET POINT ENTRY and 55 (VP-10) and tubing. SET POINT ENTRY
4.1), (TM-1). N O - Verify RBC/PLT Threshold Replace as necessary (PL- - Verify WBC Threshold is
- Clean Shear Valve (VP-48). is 550. 4.1), (TM-1). 360.
NO
- Verify 0 o and 10 o Log - Verify operation of WBC - Verify WBC Gain (VP-23).
Amplifiers (VP-28). Syringe (VP-6), (VP-7). - Replace MAM (PL-12).
- Verify 0 o and 10 o Gain (VP- - Clean Shear Valve (VP-48). - Replace SPM (PL-10).
26), (VP-27).
Problem - Replace CPU/DCM (PL-13).
Corrected
?
- Replace CPU/DCM (PL-13).
Problem
YES Corrected
?
Total Call
YES
Total Call
12 13 14
- Verify NOC Sample is being - Clean Laser Bench (VP-43). - From Main Menu, press
staged to Optical Flow Cell. - Clean, debubble, and wet DIAGNOSTICS
- Verify operation of Valve 41 Optical Flow Cell (VP-44), F12
(VP-10) and tubing. Replace as (VP-45).
F1
necessary (PL-4.1), (TM-1). - Verify beam alignment (VP-
SET POINT ENTRY
- From Main Menu, press 20), (VP-21).
- Verify RBC/PLT Threshold is
DIAGNOSTICS - Replace Optical Flow Cell
550.
F12 (PL-10).
- Verify 0 o and 10 o Log Amplifiers
F1 - Replace MAM (PL-12).
(VP-28).
SET POINT ENTRY - Replace CPU/DCM (PL-13).
- Verify 0 o and 10 o Gain (VP-26),
- Verify NOC Threshold is 400. (VP-27).
- Perform NOC Gain Adjustment - Replace MAM (PL-12).
(VP-25). - Replace SPM (PL-10).
15 16

- Verify operation of WBC - From Main Menu, press
DIAGNOSTICS
Syringe (VP-6), (VP-7). DIAGNOSTICS
F12
- Clean Shear Valve (VP-48). F12
F1
F1
NO SET POINT ENTRY
SET POINT ENTRY
- Verify WBC Threshold is
360. - Verify NOC Threshold is
400.
- Verify WBC Gain (VP-23).
- Perform NOC Gain
- Replace MAM (PL-12). Adjustment (VP-25).
Problem
Corrected - Replace SPM (PL-10).
?
YES
Total Call
17
- Verify stable laser power

(VP-46).
- Verify samples are being staged to - Clean, debubble, and wet
Optical Flow Cell by Peri-pump. Optical Flow Cell (VP-44),
Troubleshoot as necessary. YES
(VP-45).
- Verify Sheath Reservoir is filling - Replace Optical Flow Cell
properly. (PL-10).
- Verify Pressure 2 (VP-16) and Power Stable - Replace MAM (PL-12).
proper Sheath flow to Optical Flow NO
Cell. Troubleshoot as necessary. ? - Replace SPM (PL-12).
- Replace In-Line Filter (PL-4.2). - Replace CPU/DCM (PL-13).
- Replace Vent Accumulator Filter NO
(PL-4.2).
- Verify operation of Sample - Connect new Laser Head to
Injection Syringe (VP-6), (VP-7). Laser Power Supply and
Troubleshoot as necessary. verify power (See Warning).
If good, replace Laser Head
NO (PL-10). If not, replace Laser
Power Supply (PL-2).
Problem 'DQJHU=#+LJK#9ROWDJH#00#$VVXUH#WKH#SRZHU#WR#WKH#
'DQJHU
Corrected LQVWUXPHQW#LV#RII1#$#KLJK#YROWDJH#FKDUJH#PD\#UHPDLQ#RQ#
? WKH#ODVHU#FRQQHFWRU#ZLWK#WKH#SRZHU#RII1#'R#QRW#WRXFK#WKH#
PDOH#SLQV1#6KRUW#PDOH#SLQV#WR#&KDVVLV1
YES
Total Call
18
- From Main Menu,
press - From Main Menu, press
DIAGNOSTICS DIAGNOSTICS
F12 F12
- Verify RBC/PLT Sample is being F1 F1
staged to Optical Flow Cell. SET POINT ENTRY
SET POINT ENTRY
- Verify operation of Valve 54 (VP-10) - Verify WBC Threshold is
and tubing . Replace as necessary - Verify RBC/PLT
Threshold is 550. 360.
(PL-4.1) , (TM-1) . NO
- Replace MAM (PL-12) .
- Clean Shear Valve (VP-48) .
19 - Replace SPM (PL-10) .
NO - Replace CPU/DCM (PL-13) .
- Verify operation of WBC 20

Syringe (VP-6), (VP-7).
- Clean Shear Valve (VP-48) .
Problem
Corrected - Verify NOC Sample is being staged
? to Optical Flow Cell.
- Verify operation of Valve 41 (VP-10)
YES and tubing. Replace as necessary
Problem
(PL-4.1) , (TM-1) .
Corrected
Total Call - From Main Menu, press
?
DIAGNOSTICS
F12
YES
F1
SET POINT ENTRY
Total Call - Verify NOC Threshold is 400.
5#7URXEOHVKRRWLQJ IP-7 Reagent Empty
IP-7 Reagent Empty (Page 1 of 5)
Start
- Open Right Front Door

HGB Lyse
YES and observe HGB/
Empty
- Verify reagents, type and WBC Syringe.
?
expiration.
- Ensure reagent
NO
containers are connected YES 1
to the correct tubing and
input ports.
- Ensure sinkers are on WBC Lyse
lines and lines are at Empty
bottom of containers. ?
- Ensure reagent lines are
not crimped or clogged. NO
- INITIALIZE and PRIME
instrument.
- Open Left Front Door
Diluent/Sheath
YES and observe Diluent and
Empty
Sheath Reservoirs.
?
NO 2
Total Call
1
Syringe
Filling YES
Properly
From Main Menu, YES ?
press - Remove Top Cover and
RUN observe Reagent Sensor
Board. See Figure
CLEAR FAULT NO
- Inspect and seat
connectors on J1 of
- Verify operation and tubing Reagent Sensor Board
in valves 28 for HGB, 23 and J26 of FCM.
Syringe for WBC. (VP-10) See - Replace FCM (PL-14.1).
Moving Figure. Replace as
Properly necessary (PL-4.1), (TM-1).
?
- Replace SDM2 (PL-15)
Floss Tubing
NO Up and Down
Ensure Tubing
is Seated in Slot
- Clean/replace syringe (PL-7).

- Verify operation and tubing in
- Verify Syringe Driver
valves 24 for HGB, 25 for WBC.
operation (VP-6), (VP-7).
(VP-10) See Figure. Replace as
- Replace Syringe Driver necessary (PL-4.1), (TM-1).
Assembly (PL-7), (RR-11). 32098
- Replace SDM2 (PL-15).
- Replace MPM (PL-11).
Note: The "Diluent/Sheath Empty"

Diluent message can be generated by the In-line
Not Filling YES 5 Diluent/Sheath Sensor, the Diluent
Properly Reservoir Level Sensor, or the Sheath
RUN Reservoir Level Sensor.
?
CLEAR FAULT
- Observe the Diluent and Sheath NO
Reservoirs and verify that both fill to
the Sensor Electrodes and
immediately stop. See Figure Sheath
Not Filling YES 6
NO Properly
?
Both NO
Filling YES 3
Properly
?
Diluent Filling
YES 7
Past Electrode
NO YES
?
Neither NO Fill
Filling To
H e re
Properly
?
Sheath Filling
Past Electrode 32099
YES
?
4
3 4 5 6
- Ensure Vac 1 line to

- Ensure Vac 1 line to reservoir reservoir is free of
- Remove Top Cover and - Ensure Diluent/Sheath plumbing restrictions.
is free of restrictions. is free of restrictions.
observe Reagent Sensor
- Ensure there is no saline - Ensure there is no saline
Board. See Figure - Ensure there is no saline bridge at
bridge at reservoir connector. bridge at reservoir
- Inspect and seat reservoir connectors. connector.
connectors on J1 of - Verify the Vac 1 level (VP-16) - Verify operation of Valves 62
and 63 (VP-10). Replace as - Verify operation of Valves 61
Reagent Sensor Board and verify Vac 1 retention (VP-
necessary (PL-4.1) and 64 (VP-10). Replace as
and J26 of FCM. 15). necessary (PL-4.1).
- Replace FCM (PL-14.1). - Ensure Vac 1 lines to reservoirs is - Verify operation of Check
Valve Replace as necessary - Verify operation of Check
free of restrictions. Valve. Replace as
(TM-1).
- Verify operation of Valves 61, 62, necessary (TM-1).
63, and 64 (VP-10). Replace as - Verify Reservoir is not
cracked or leaking Replace - Verify Reservoir is not
necessary (PL-4.1). cracked or leaking. Replace
as necessary (PL-4.2).
- Verify operation of Check Valves. as necessary (PL-4.2).
Replace as necessary (TM-1). - Replace FCM (PL-14.1).
- Replace FCM (PL-14.1).
- Verify Reservoirs are not cracked
or leaking. Replace as necessary
(PL-4.2).
- Clean sensor contacts

(Electrodes and
connector) at top of
Reservoir.
- Remove pins 1 and 4
and clean and crimp
metal contacts so they
are touching. See Figure
- If pins 1 and 4 are
damaged, replace with
pins 2 and 3. 32100
- Reseat Sensor Cables

(CDM 2 J8 for Diluent, or
CDM 2 J10 for Sheath.
5#7URXEOHVKRRWLQJ IP-8 Optical Backgrounds Too High (Page 1 of 1)
IP-8 Optical Backgrounds Too High (Page 1 of 1)
Start
- From Main Menu, press - Perform the following in order and verify
- Verify Background Counts backgrounds after each action taken.
DIAGNOSTICS
(VP-56). - Perform Extended Autoclean.
F12
NO - Clean Shear Valve (VP-48).
F1
- Clean and debubble Optical Flow Cell
SET POINT ENTRY
(VP-44).
WBC Only - Verify RBC/PLT Threshold is 550.
- Clean/replace Diluent and Sample
Too High - From Main Menu, press Injection Syringes (PL-7).
? SPECIAL PROTOCOLS - Replace In-line Sheath Filter (PL-4.2).
MORE - Ensure RBC/PLT Mixing Chamber is
REAGENT RESERVOIR NO being rinsed and drained properly.
YES
- Follow on-screen instructions and press - Clean Laser Bench (VP-43).
EMPTY DIL/SHEATH
- From Main Menu, press - Replace Diluent Container and press
DIAGNOSTICS FILL DIL/SHEATH
F12 - Run multiple backgrounds and verify.
F1
SET POINT ENTRY
- Verify WBC Threshold is 360.
- Replace WBC Lyse Container. Backgrounds
- Clean/replace WBC Syringe (PL-7). OK
- Ensure WBC Mixing Chamber is ?
being rinsed and drained properly.
- INITIALIZE and PRIME Instrument. YES
Total Call
5#7URXEOHVKRRWLQJ IP-9 Histogram Problems (Page 1 of 1)
IP-9 Histogram Problems (Page 1 of 1)
Start
- Perform the following in order
and verify histograms after
- Verify reagents; type and each action taken. WBC Only YES
expiration date. - Ensure RBC/PLT and/or
WBC Mixing Chambers are ?
- Ensure reagents are at
room temperature. being rinsed and drained
properly.
- Verify Backgrounds (VP-56).
- Perform Extended Autoclean. NO - Verify WBC Gains
- Clean Shear Valve (VP-48). (VP-23).
- Clean, debubble, and wet
Backgrounds Go to Optical Flow Cell (VP-44), - Verify 0 o and 10 o Log
NO
OK (IP-8) N O (VP-45). Amplifiers (VP-28).
NO
? - Fine align Optical Flow Cell - Verify 0 o and 10 o Gains
(VP-20). (VP-26), (VP-27).
YES
- Replace WBC Lyse for WBC - Clean/replace Diluent, WBC
problem or Diluent for RBC/ Lyse, and Sample Injection
PLT problem. Syringes (PL-7).
- Replace In-line Sheath Filter
(PL-4.2).
Problem
Corrected
Problem
? Corrected
?
YES
Total Call YES Total Call
5#7URXEOHVKRRWLQJ IP-10 Controls Out
IP-10 Controls Out (Page 1 of 2)
Start
- Verify reagents; type and NO Q.C. OK YES

expiration date.
?
- Ensure reagents are at room
temperature and have not Total Call
been previously frozen. - Perform Autoclean.
- Ensure Controls and - From Main Menu, press
Calibrators have not been SPECIAL PROTOCOLS
frozen or heat stressed. - Open fresh Commercial
MORE
Control, and run Q.C.
AUTOCLEAN
- Verify Backgrounds (VP-56).
- Check Control Lot # YES
- Use current Control Insert
Sheet to verify Q.C means/
limits are correct if customer
has not established their own Results
NO 1
means. All Acceptable
Backgrounds ?
- From Main Menu, press OK
SETUP ?
YES
Q.C. SETUP MENU
NO
Q.C. LIMITS Total Call
- Edit as necessary.
Go to (IP-8).
- Run Q.C.
5#7URXEOHVKRRWLQJ IP-10 Controls Out
IP-10 Controls Out (Page 2 of 2)
- Determine which
NO
parameter(s) are out-of-
Precision
- Clean Shear Valve (VP-48). range, and go to (IP-15)
In Spec
or (IP-16) for HGB and
? correct.
- For indices (MCH or
YES MCHC), determine which
- Verify System Precision parameter (HGB, MCV,
- From Main Menu, press - Recalibrate out-of- or RBC is imprecise and
RUN range parameter(s) troubleshoot accordingly.
SPECIMEN TYPE (VP-58). MCH = HGB X10
- Select empty Q.C. File RBC
Q.C. SPECIMEN
- Review CV% (VP-57). MCHC = HGB X 100
MCV X RBC
5#7URXEOHVKRRWLQJ IP-11 Vacuum Accumulator #1 Wet
IP-11 Vacuum Accumulator #1 Wet (Page 1 of 2)
Start
Liquid Exiting YES

Analyzer - Verify that Waste Chamber 2 is
- Reinitialize Analyzer not overflowing. If overflowing,
From Main Menu: ? verify Pressure Valve 18 (VP-10).
DIAGNOSTICS Replace as necessary (PL-4.1).
MORE Check output Check Valve.
NO Replace as necessary (TM-1).
INITIALIZATION
- Ensure operation of Waste
Chamber 2 Trap and Trap Output
- Perform Vacuum
Check Valve. Replace as
Accumulator #2 Rinsing
necessary (PL-4.2) and (TM-1).
Procedure (VP-60)
Press:
MORE
- Observe the Waste Line into
External Waste Container and
press: Problem
Corrected NO
DRAIN ACCUMULATORS
? - Perform Vacuum
YES Procedure (VP-60)
1
Total Call
- Check Cable and replace

if necessary (PL-5)
- Replace Accumulator
- Remove connector from (PL-5)
J11 on VPM and check
resistance between
connector pins. Must be
greater than 20 NO
Megohms. See Figure J10 J11
Vac 1 Acc Vac 2 Acc
VPM
BD
> 20 Megohms
YES 32003
- Replace VPM (PL-5)

- Replace FCM (PL-14.1)
Start
Liquid Exiting YES

Analyzer - Verify that Waste Chamber 2 is
- Reinitialize Analyzer not overflowing. If overflowing,
From Main Menu: ? verify Pressure Valve 18 (VP-10).
DIAGNOSTICS Replace as necessary (PL-4.1).
MORE Check output Check Valve.
NO Replace as necessary (TM-1).
INITIALIZATION
- Ensure operation of Waste
Chamber 2 Trap and Trap Output
- Perform Vacuum
Check Valve. Replace as
necessary (PL-4.2) and (TM-1).
Procedure (VP-60)
Press:
MORE
- Observe the Waste Line into
External Waste Container and
press: Problem
Corrected NO
DRAIN ACCUMULATORS
? - Perform Vacuum
YES Procedure (VP-60)
1
Total Call
- Check Cable and replace

if necessary (PL-5)
- Replace Accumulator
- Remove connector from (PL-5)
J11 on VPM and check
resistance between
connector pins. Must be
greater than 20 NO
Megohms. See Figure J10 J11
Vac 1 Acc Vac 2 Acc
VPM
BD
> 20 Megohms
YES 32003

5#7URXEOHVKRRWLQJ IP-13 HGB Problems
IP-13 HGB Problems (Page 1 of 2)
Start
"0", Low, High, or

HGB Only
Erratic
- Verify proper Reagents and
Expiration Date. ?
From Main Menu, Press:
RUN
SPECIMEN TYPE
FRAGILE WBC Both
- Run a Normal Control five
times and record the NOC
- Perform HGB Cleaning
and HGB values.
Procedure (VP-49).
- Compare the NOC and HGB - Remove, inspect, and clean Shear From Main Menu, Press:
results to the Data Sheet. Valve.
DIAGNOSTICS
- Perform Vacuum & Pressure Level
RAW DATA SUMMARY
Verification/Adjustment (VP-16).
- Run five background counts
- Verify tubing and operation of Valves
and observe the HGB Sample
51, 28, 24, 33, 93, and 26 (VP-10)
and Reference Readings.
and replace as necessary (PL-4.1).
- Verify operation of HGB Lyse and
RBC Diluent Syringes (VP-6), (VP-7)
and replace as necessary (PL-7).
5#7URXEOHVKRRWLQJ IP-13 HGB Problems
IP-13 HGB Problems (Page 2 of 2)
1 2 5
Total Call
Select path from Chart below. - Verify connector at J18 of FCM.

Reference Sample 3 - Replace HGB Flow Cell (PL-4.2).
2
1950-2050 1950-2050 - Replace FCM (PL-14.1).
3 - Replace CPU/DCM (PL-13).
< 1950 < 1950
> 2050 >2050 3 - Perform HGB Adjustment

(VP-5)
Erratic 1950-2050 4
1600 1950-2050 4 4
Erratic Erratic 5
0 0
5
- Verify tubing, operation (VP-10),

and flow through Valve 94 and
replace as necessary (TM-1),
(PL-4.1).
5#7URXEOHVKRRWLQJ IP-14 X-B Errors
IP-14 X-B Errors (Page 1 of 3)
Start Effect Of RBC, MCV, & HGB On X-B Parameters

X-B Parameter RBC RBC MCV MCV HGB HGB
MCV NA NA NA NA
- Clean Fan Filters and verify all Fans are running
properly. MCH NA NA
- Ensure there is at least 6" of clearance behind and MCHC
on the left side of Instrument.
- Ensure Instrument is not subject to drastic
temperature variations (direct sunlight, heating/air
conditioning ducts).
- Review Customer Maintenance Log.
- If Fan Filters were clogged and then cleaned, allow a NO
Controls at or - Review Maintenance Procedures and
15 minute temperature stabilization period.
Near 2 SD Dates.
- Review patient result in Datalog, and look for groups of mean - Review X-B Results.
( 3 or more) of abnormal MCV, RBC, or HGB.
? - Determine if Maintenance was
-If MCV shifts, ask Customer for possible causes,
performed just before X-B Shift.
such as Oncology or Geriatric Patients.
If MCH or MCHC errors: YES
- See table to determine which parameter (HGB,
RBC, MCV) is bad. Go to (IP-10)
Note: HCT = RBC X MCV/10 Controls Out. 1
MCH = HGB X 10/RBC
MCHC = (HGB X 100)/(RBC X MCV)
- Review Q.C. Logs for all 3 Control Levels.

- Verify RBC, HGB, and MCV Control Levels are not
at the high or low end of range.
- Verify Reagent, Type and

Expiration Date.
Shift
After
Maintenance
?
Reagent
Change YES
YES Required
?
- Monitor X-B for 5
- Repeat Maintenance Data Points (100
Procedures NO Samples).
performed before X-B X-B In
Shift. Range
2
- Monitor X-B for 5 Data ?
Points (100 Samples).
YES
X-B In
Range NO
Total Call
?
YES 2
Total Call
- Verify System Precision

RUN
SPECIMEN TYPE
- Select empty Q.C. File
Q.C. SPECIMEN
- Run one Patient 10
times.
- Review CV% (VP-57) .
All
Parameters YES
OK
?
- Verify Calibrator Insert
Sheet matches Current
NO Calibrators.
- Perform System
Calibration (VP-58) .
- Determine which
parameter(s) are out-of-
range, and go to (IP-15) or
(IP-16) for HGB and correct.
5#7URXEOHVKRRWLQJ IP-15 Optical Parameters Imprecision
IP-15 Optical Parameters Imprecision (Page 1 of 3)
Start
- Verify Background Counts

- Clean Fan Filters and verify all Fans are (VP-56).
running properly.
- Ensure there is at least 6" of clearance
behind and on the left side of Instrument. - Verify System Precision.
- Ensure Instrument is not subject to drastic - From Main Menu, press Backgrounds
temperature variations (direct sunlight, RUN OK
heating/air conditioning ducts). SPECIMEN TYPE ? NO Go to (IP-8).
- If Fan Filters were clogged and then NO
- Select empty Q.C. File YES
cleaned, allow a 15 minute temperature
Q.C. SPECIMEN
stabilization period.
- Run one Patient 10 times. All
- Perform Autoclean
- Review CV% (VP-57). Imprecise
? YES 2
SPECIAL PROTOCOLS
MORE NO
AUTOCLEAN Problem
- Clean Shear Valve (VP-48). Corrected
RBC/PLT
- Manually clean, debubble, and wet Optical ? Only
Flow Cell (VP-44), (VP45). YES 3
?
- Clean Optics Bench (VP-43). YES
NO
- Replace In-line Sheath Filter (PL-4.2).
Total Call 1
1 2
- Replace WBC Lyse Container.

YES - Verify samples are being staged to
DIAGNOSTICS
Optical Flow Cell by Peri-pump.
WBC F12 Troubleshoot as necessary.
Only F1 - Verify Sheath Reservoir is filling
? SET POINT ENTRY properly.
- Verify WBC Threshold is 360. - Verify Pressure 2 (VP-16) and
NO - Ensure WBC Mixing Chamber is proper Sheath flow to Optical Flow
being rinsed and drained Cell. Troubleshoot as necessary.
properly. - Verify operation of Sample
NOC - Verify operation of WBC Lyse Injection Syringe (VP-6) , (VP-7 ).
Only Syringe (VP-6) , (VP-7) . Troubleshoot as necessary.
? Troubleshoot as necessary. - Replace Optical Flow Cell (PL-10) .
- Verify/adjust WBC Gain (VP-23) . - Replace Shear Valve Sections
YES - Replace Shear Valve Sections (PL-9.1) .
(PL-9.1) . - Replace MAM (PL-12) .
- Ensure HGB Mixing - Replace MAM (PL-12) . - Replace SPM (PL-10) .
Chamber is being rinsed NO - Replace SPM (PL-10) . - Replace CPU/DCM (PL-13) .
and drained properly. - Replace CPU/DCM (PL-13) .
Total Call
- Replace Diluent Container.

DIAGNOSTICS
F12
F1
SET POINT ENTRY
- Verify RBC/PLT Threshold is 550.
- Ensure RBC/PLT Mixing Chamber is
being rinsed and drained properly.
- Verify 0 o and 10 o Log Amplifiers (VP-28).
- Verify 0 o and 10 o Gain (VP-26), (VP-27).
- Replace Shear Valve Sections (PL-9.1).
5#7URXEOHVKRRWLQJ IP-16 HGB Imprecision (Page 1 of 1)
IP-16 HGB Imprecision (Page 1 of 1)
Start
HGB CVs
- Clean Fan Filters and verify all Fans are - Ensure HGB Flow Cell is being
OK
running properly. rinsed, drained, and vented
? properly. Verify Valves 51, 94, 93,
- Ensure there is at least 6" of clearance
behind and on the left side of (VP-10) and tubing . Replace as
YES necessary (PL-4), (TM-1).
Instrument. NO
- Ensure Instrument is not subject to - Clean HGB Lyse Syringe.
Total Call
drastic temperature variations (direct - Verify HGB Lyse Syringe (VP-6),
sunlight, heating/air conditioning ducts). (VP-7). Replace as necessary
- If Fan Filters were clogged and then NO (PL-7).
cleaned, allow a 15 minute temperature - Inspect and re-seat Cable at J18
- Replace HGB Lyse
stabilization period. of FCM (PL-4.2).
Container>
- Clean Shear Valve (VP-48). - Replace HGB Flow Cell (PL-4.2).
- Manually clean HGB Flow Cell (VP-49). - Replace FCM (PL-14.1).
NO
- Verify System Precision. - Replace CPU/DCM (PL-13).
RUN
HGB CVs
SPECIMEN TYPE OK
- Select empty Q.C. File
?
Q.C. SPECIMEN
- Run one Patient 10 times. YES
- Review HGB CV% (VP-57).
Total Call
5#7URXEOHVKRRWLQJ IP-17 Primary Vacuum/Pressure Isolation,
IP-17 Primary Vacuum/Pressure Isolation, (Page 1 of 9)
Start
5P SI 12"H g 8"Hg 12P SI 12"H g

7
To S L
6
Pres To S L
- Remove Left Side Cover and Constantly
5
extend Air Supply (RR-7) to Running
FLO W PA N E L
observe pumps. (Vac-Front, ? YES
Pres-Rear)
TO
NO
2
4
9P SI
- Reinitialize Analyzer NO Vac
12P SI
From Main Menu: Constantly
Running
DIAGNOSTICS YES 3 2 00 4
MORE ?
INITIALIZATION
5
NO
Both
- Disconnect tubing from
Constantly
positions 6 and 7 on Air
Running
Supply Manifold, and
? observe Pumps and VPM
Pump LEDs. See Figures
YES
32005
- Observe LEDs. See
1
Figure 6
All Valves
Close Then NO
Open
Both LEDs ?
ON 2
? +12 VNR
YES
Fuse (Top)
NO 32006
- Check +12V UR and Fuse.

- Replace Pump Relay See Figure
Board (PL-5) YES - Replace if necessary (PL-1)
Check fuse again
NO
- Reinitialize Analyzer
Problem Fuse
From Main Menu: YES
Corrected Blown
DIAGNOSTICS
? ?
MORE
INITIALIZATION NO 8
- Observe Flow Panel YES
valves - Replace Power Supply
Total Call (PL-1)
- Check +24V UR (28.9V- Note: If the +24 UR is approximately

37.7V) and Fuse the same as the +12 UR, the +24V
(Bottom Fuse) Fuse is blown or the +24V Power
- While observing VPM - Replace Fuse if Supply is defective.
Solenoid LEDs (See necessary (PL-1)
Figure) press:
INITIALIZATION
NO
NO
- Check Fuse again

Problem
Corrected
LEDs Bright ?
Then Dim When
Activated Fuse
YES Blown YES
?
?
Total Call
YES NO 8
3 - Replace Power Supply

(PL-1) 32007
7
To S L
5P SI 12"H g 8"H g 12P SI 12"H g

3
6
To S L
5
- Unpinch tubing
FL O W PA N E L
- Pinch tubing as shown in - Pinch tubing at positions
Figure 6 and 7 that supply 12
TO
YES psi and 12" Hg to Sample
Loader
P inch
9P SI
Pumps
12P SI
Stop
? 32008
Pumps
NO NO
Stop
?
- Inspect 12 psi and 12" Hg

plumbing and - Ensure Valves 31 and 32,
YES 97 and 98, 17 and 18 are
Accumulators. Replace
as necessary (PL-5), not open at the same
(TM-2) time.
- Inspect Vent Valves 1 and - Inspect Sample Loader - Inspect Flow Panel 12 psi
4. Replace as necessary plumbing and 3-Way and 12" Hg plumbing for
(PL-5) Valves. Replace as leaks.
necessary (PL-18.3),
(TM-3)
Pump YES - Unpinch tubing and pinch

Stops at position 6 on Manifold.
?
- Observe Pressure Pump
LED. See Figure Page 1
NO
Pump
Stops
- Inspect 12 psi plumbing ? NO

LED and Accumulator.
ON Replace as necessary
(PL-5), (TM-2) - Inspect Flow Panel 12 psi
?
- Inspect Vent Valve 1. plumbing for leaks.
Replace as necessary YES
NO (PL-5)
7
To S L
5P S I 12"H g 8"H g 12P S I 12"H g

YES
6
To S L
- Replace Pump Relay
Board (PL-5)
5
4
FL O W PA N E L
- Inspect Sample Loader
TO
plumbing and 3-Way
- Pinch tubing See Figure Valves. Replace as
9P S I
this page necessary (PL-18.3), P inch
(TM-3)
12P S I
32 0 09
Pump YES - Unpinch tubing and pinch

Stops at position 7 on Manifold.
?
- Observe Vacuum Pump
LED. See Figure Page 1
NO
Pump
Stops
? NO
- Inspect 12" Hg plumbing
LED and Accumulator.
ON Replace as necessary
(PL-5), (TM-2) - Inspect Flow Panel 12"
?
- Inspect Vent Valve 4. Hg plumbing for leaks.
Replace as necessary YES
NO (PL-5)
7
- Replace VPM (PL-5) To S L
5P SI 12"H g 8"H g 12P SI 12"H g

YES
6
To S L
- Replace Pump Relay
Board (PL-5)
5
4
FLO W PAN E L
3
Pinch
TO
plumbing and 3-Way
2
- Pinch tubing. See Figure Valves. Replace as
9P SI
1
(TM-3)
12P SI
32010
6
- Replace VPM
NO
(PL-5)
- Reseat connectors VPM J18, PRM

J1, J2, J3, J4
Neither - Check Fuse on PRM. Replace if
LEDs
Pump YES YES necessary (PL-5)
ON
Runs
? - Verify 115VAC to PRM
?
- Replace VPM - Replace PRM (PL-xx)
NO
(PL-5)
NO
VAC - Reseat connectors VPM J18, PRM

VAC LED J1, J3
Doesn't YES
ON
Run YES - Check 115VAC across Pins 1 and 2
? on PRM P3. IF present, replace Vac
?
Pump. If not, replace PRM (PL-5)
- Replace VPM
NO NO (PL-5)
- Reseat connectors VPM J18,

PRM J1, J2
Pres
Doesn't Pres LED - Check 115VAC across Pins 1 and
YES YES
Run ON 2 on PRM P2. IF present, replace
? Pres Pump. If not, replace PRM
? (PL-5)
Both NO - Replace Pump Head

OK - If problem persists,
- Re-connect tubing to replace Pump Assembly
positions 6 and 7 (6-Red, ?
Pump Head:
7-Green)
Pressure (PL-5)
YES Vacuum (PL-5)
Pump Assy:
- Perform Vacuum and - Observe Vacuum and Pressure (PL-5)
Pressure Level Pressure Pumps and Vacuum (PL-5)
Verification/Adjustment time the duration between
(VP-16) run cycles.
- Perform Vacuum and NO

Pres Vac
Pressure Recovery Cycles Cycles
Verification (VP-17) < 2 min < 2 min NO
YES ? ?
Total Call
See Pressure Leaks (IP-18) See Vacuum Leaks (IP-19) YES
8
C D M 2 & S D M /C D M P o w er C on n ecto rs
J3 J1
+2 4 V U R
5 1
J1 B a ck Fro m +1 2 V U R To
UR RET P.S . 4 2
1 UR RET SDM A
To 3 J1/J2 3 3
C D M 2-J3
4 +1 2 V U R 1
FC M -J5
5 +2 4 V U R
- If the Fuse continues to blow, J2

UR RET
assemblies on the +12V UR or 1
To 3 PSM J2
+24V UR must be disconnected S D M /C D M -J3 4 +1 2 V U R 1
one at a time until the shorted 5
+2 4 V U R To
2 SDM B
assembly is found and
J3 3
replaced. (See Figures)
To +2 4 V U R
1
- Power-down instrument and S h ea r
2 RET
Valve
connect Meter between TP2- D rive r-J3 J4
+12V or TP1-+24V and TP4 on
N o te: J4 an d J5 a re no t o n 1
PSM and remove connectors S D M /C D M
To
2 SDM C
until short is found
3
- Replace defective assembly and J4
+2 4 V U R
verify proper operation 1
To +1 2 V U R FC M
2
S D M 9 -J1 0
3 UR RET
J5
1
To
2 SDM D
3 2 0 11 3
5#7URXEOHVKRRWLQJ IP-18 Pressure Leak Isolation
IP-18 Pressure Leak Isolation (Page 1 of 7)
Note: This procedure is designed to isolate leaks in the pressure plumbing. Refer to IP-17 Primary Vacuum/
Pressure Isolation for troubleshooting gross pressure failures.
Start
- Observe Pressure Pump

and time the duration
- Remove Left Side Cover and between run cycles.
extend Air Supply (RR-7) to
observe Pres Pump. (Vac-
Front, Pres-Rear)
- Reinitialize Analyzer Pump

From Main Menu: Cycles
DIAGNOSTICS < 2 min YES
MORE ?
INITIALIZATION
NO
1
Total Call
- Adjust Pressure Levels if
necessary (VP-16)

Figure) press:
INITIALIZATION
NO
NO
- Check Fuse again

Problem
Corrected
LEDs Bright ?
Then Dim When
Activated Fuse
YES Blown YES
?
?
Total Call
YES NO 7 3 2 0 07

(PL-1)
- Select 3 pressure levels in

real-time press:
MORE
VOLTAGE READINGS
Move cursor to each level
and press:
SELECT
When finished press:
FINISH SELECT
- Observe the 3 levels and

verify that none decrease
more than 0.2 psi in 1
minute.
- Pinch tubing at position 3

of Air Supply Manifold. - Inspect tubing between
NO
See Figure Air Supply and Flow
Pres Panel.
3 - Inspect plumbing through
OK YES
Valve 63 to Diluent
? Reservoir.
- Inspect Diluent Reservoir
YES Pres for leaks. Replace as
3 necessary (PL-4.2).
OK
Pres ?
2
OK NO
5P SI 12"H g 8"H g 12P SI 12"H g

7
To S L
? NO
6
To S L
5
YES
- Inspect 5 psi Accumulator
FL O W PA N E L
and plumbing on Air
Supply. Replace as
TO
Pres necessary (PL-5), (TM-2)
1
OK NO
9P SI
?
12P SI
5
32004
YES Total Call
4 5
- Inspect tubing between

Air Supply and Flow
Panel. - Pinch tubing See Figure
- Pinch tubing at position 2
of Air Supply Manifold. - Inspect plumbing through
See Figure Page 2. Valve 64 to Sheath
YES Reservoir.
- Inspect Sheath Reservoir
for leaks. Replace as Pres
necessary (PL-4.2). 1 YES
OK
?
Pres
2
7
To S L
5P SI 12"H g 8"H g 12P SI 12"H g

OK 6
?
6
To S L NO
5
NO
4
FL O W PA N E L
- Inspect Valve 1. Replace
as necessary (PL-5).
3
TO
- Inspect 12 psi plumbing
- Inspect 9 psi Accumulator and Accumulator.
2
and plumbing on Air

9P SI
Replace as necessary
Supply. Replace as P inch
(PL-5), (TM-2).
necessary (PL-5), (TM-2)
1
12P SI
32009
- Inspect plumbing to
Valves 32, 98, and 18.
- Press in on Valves 32, 98,
- Unpinch tubing and pinch and 18 one at a time, if
at position 6 on Manifold. leaking stops replace as
NO
necessary (PL-4.1)
Pres
1
OK
?
YES

plumbing and 3-Way
Valves. Replace as
(TM-3)
7
C D M 2 & S D M /C D M P o w er C on n ecto rs
J3 J1
+2 4 V U R
5 1
UR RET P.S . 4 2
1 UR RET SDM A
To 3 J1/J2 3 3
C D M 2-J3
4 +1 2 V U R 1
FC M -J5
5 +2 4 V U R
J2
- If the Fuse continues to blow, UR RET
1
assemblies on the +12V UR or To 3 PSM J2
+24V UR must be disconnected S D M /C D M -J3 4 +1 2 V U R 1
+2 4 V U R To
5 2
one at a time until the shorted SDM B
assembly is found and replaced. J3 3
(See Figures) To +2 4 V U R
S h ea r 1
2 RET
- Power-down instrument and Valve
D rive r-J3 J4
connect Meter between TP2-
+12V or TP1-+24V and TP4 on N o te: J4 an d J5 a re no t o n 1
To
S D M /C D M 2
PSM and remove connectors SDM C
until short is found 3
J4
+2 4 V U R
- Replace defective assembly and To
1
+1 2 V U R FC M
verify proper operation 2
S D M 9 -J1 0
3 UR RET
J5
1
To
2 SDM D
3 2 0 11 3
5#7URXEOHVKRRWLQJ IP-19 Vacuum Leak Isolation
IP-19 Vacuum Leak Isolation (Page 1 of 6)
Note: This procedure is designed to isolate leaks in the vacuum plumbing. Refer to IP-17 Primary Vacuum/
Pressure Isolation for troubleshooting gross vacuum failures.
Start
- Observe Vacuum Pump

and time the duration
- Remove Left Side Cover and between run cycles.
extend Air Supply (RR-7) to
observe Vac Pump. (Vac-
Front, Pres-Rear)
- Reinitialize Analyzer Pump

From Main Menu: Cycles
< 2 min YES
DIAGNOSTICS
MORE ?
INITIALIZATION
1
NO
Total Call
- Adjust Vacuum Levels if

necessary (VP-16)

Figure) press:
INITIALIZATION
NO
NO
- Check Fuse again

Problem
Corrected
LEDs Bright ?
Then Dim When
Activated Fuse
YES Blown YES
?
?
Total Call
YES NO 5
3 2 0 07

(PL-1)
- Select 2 vacuum levels in

real-time press:
MORE
VOLTAGE READINGS
Move cursor to each level
and press:
SELECT
When finished press:
FINISH SELECT
- Observe the 2 levels and

verify that none decrease
more than 0.2 psi in 1
minute.
- Pinch tubing at position 5 - Inspect tubing between Air Supply and

of Air Supply Manifold. Flow Panel.
NO See Figure
- Inspect plumbing through Valve 17 to
Vac WC-2.
2 YES - Inspect WC-2 for leaks. Replace as
OK
necessary (PL-4.2).
?
- Inspect plumbing to Valve 12.
Vac - Press in on Valve 12. Replace as
YES necessary (PL-4.1).
2
OK
?
Vac
5P SI 12"H g 8"H g 12P SI 12"H g

7
To S L
1
OK NO NO
6
To S L
?
5
4 - Inspect Vac 2
FL O W PA N E L
YES Accumulator and
plumbing on Air Supply.
TO
Total Call (PL-5), (TM-2)
9P SI
1
12P SI
32004
- Unpinch tubing and

pinch at position 7 on
Manifold. - Inspect plumbing to
- Pinch tubing See Figure Flow Panel.
- Inspect plumbing to
YES NO Valves 31 and 97.
- Inspect WC-3 and WC-
Vac 4 for leaks. Replace as
1 necessary (PL-4.2)
Vac OK - Press in on Valves 13,
1 ? 14, 21, 36, and 37 one
OK at a time. Replace as
? necessary (PL-4.1).
7
To S L
5P SI 12"H g 8"H g 12P SI 12"H g

NO
6
To S L
5 YES
- Inspect 12" Hg
FL O W PA N E L
4
plumbing and
Accumulator. Replace
3
P inch
as necessary (PL-5), TO - Inspect Sample Loader
(TM-2) plumbing and 3-Way
Valves. Replace as
2
9P SI
- Inspect Vent Valve 4.

(TM-2)
1
12P SI
(PL-5)
32010
5 C D M 2 & S D M /C D M P o w er C on n ecto rs
J3 J1
+2 4 V U R
5 1
UR RET P.S . 4 2
1 UR RET SDM A
To 3 J1/J2 3 3
C D M 2-J3
4 +1 2 V U R 1
FC M -J5
5 +2 4 V U R
J2
UR RET
1
To 3 PSM J2
S D M /C D M -J3 4 +1 2 V U R 1
- If the Fuse continues to blow, +2 4 V U R To
5 2
assemblies on the +12V UR or +24V SDM B
UR must be disconnected one at a J3 3
To +2 4 V U R
time until the shorted assembly is S h ea r 1
found and replaced. (See Figures) Valve 2 RET
D rive r-J3 J4
- Power-down instrument and connect
N o te: J4 an d J5 a re no t o n 1
Meter between TP2-+12V or TP1- To
S D M /C D M 2
+24V and TP4 on PSM and remove SDM C
3
connectors until short is found J4
+2 4 V U R
- Replace defective assembly and To
1
+1 2 V U R
2 FC M
verify proper operation S D M 9 -J1 0
3 UR RET
J5
1
To
2 SDM D
3 2 0 11 3
5#7URXEOHVKRRWLQJ IP-20 Shear Valve Position Fault
IP-20 Shear Valve Position Fault (Page 1 of 2)
Start Note: If the +24 UR

is approximately
- Check +24V the same as the
- Verify +24V UR (28.9V- +12 UR, the +24V
37.7V) (VP-1). Fuse (Bottom
Fuse). Fuse is blown or
- Remove, inspect, and clean the +24V Power
NO - Replace Fuse if
Shear Valve (VP-48). Supply is defective.
necessary (PL-1).
28.9V-37.7V
?
From Main Menu, press NO 28.9V-37.7V NO
DIAGNOSTICS ?
YES
MORE
INITIALIZATION - Check Fuse again.
YES
- Verify +24V between Pins 1
and 2 of Shear Valve Driver
Board. If not present re-seat - Verify proper operation.
cable.
- Replace cable. (PL-1). Fuse
Problem Blown
- Re-seat cable from FCM-J8 YES
Corrected
to SVDM-J2. Total Call ?
? - Lubricate Shear Valve Driver
(VP-42).
YES NO 1
- Replace Shear Valve Driver
Assy. (PL-9.1).
Total Call - Replace FCM (PL-14.1). - Replace Power Supply
(PL-1).
5#7URXEOHVKRRWLQJ IP-20 Shear Valve Position Fault
IP-20 Shear Valve Position Fault (Page 2 of 2)
IP-20 Shear Valve Position Fault, 2 of 2
1
C D M 2 & S DM /C D M P o w er C on n ecto rs
J3 J1
+2 4 V U R
- If the Fuse continues to blow, J1 Fro m
5 1
B a ck +1 2 V U R To
assemblies on the +12V UR or 1
UR RET P.S . 4 2
SDM A
To UR RET
+24V UR must be 3 J1/J2 3 3
C D M 2-J3
4 +1 2 V U R 1
disconnected one at a time FC M -J5
5 +2 4 V U R
until the shorted assembly is
found and replaced. (See J2
UR RET
Figures) 1
To 3 PSM J2
- Power-down instrument and S D M /C D M -J3 4 +1 2 V U R 1
connect Meter between TP2- +2 4 V U R To
5 2 SDM B
+12V or TP1-+24V and TP4 on 3
J3
PSM and remove connectors To +2 4 V U R
1
until short is found. S h ea r
2 RET
Valve
- Replace defective assembly and D rive r-J3 J4
verify proper operation. N o te: J4 and J5 a re no t o n 1
To
S D M /C D M 2 SDM C
3
J4
+2 4 V U R
1
To +1 2 V U R FC M
2
S D M 9 -J1 0
3 UR RET
J5
1
To
2 SDM D
3 2 0 11 3
5#7URXEOHVKRRWLQJ IP-21 Flags
IP-21 Flags (Page 1 of 2)
Start
Problem
- Ensure Customer has verified YES
Corrected
an Instrument problem and
?
not a Specimen Specific
problem with multiple Manual Total Call
Differential Counts.
NO
- Clean Fan Filters and verify
all Fans are running properly.
- Ensure there is at least 6" of - Perform Autoclean
- Verify Background - Run a Normal Patient
clearance behind and on the From Main Menu, press Counts (VP-56). Sample exhibiting flags 5
left side of Instrument.
SPECIAL PROTOCOLS times and print the
- Ensure Instrument is not results.
MORE YES
subject to drastic temperature
AUTOCLEAN - Observe the results and
variations (direct sunlight,
refer the the System
heating/air conditioning - Clean Shear Valve (VP-48).
Flags Table.
ducts). - Manually clean, debubble, and wet Backgrounds
- Initialize and Prime Optical Flow Cell (VP-44), (VP45). NO OK
Instrument - Clean Optics Bench (VP-43). ?
- If Fan Filters were clogged 1
- Replace In-line Sheath Filter (PL-4.2).
and then cleaned, allow a 15 NO
minute temperature
stabilization period. Go to (IP-8).
- Replace RBC Diluent (RBC/
PLT Flags) or WBC Lyse Problem
(WBC Flags) Container. YES Corrected
?
Total Call
5#7URXEOHVKRRWLQJ IP-21 Flags
IP-21 Flags (Page 2 of 2)
Flagging
WBC Flags Only All Flagging
Parameters
?
RBC and/or PLT Flags Only - Verify Pressure 2 (VP-16) and

proper Sheath flow to Optical
Flow Cell. Troubleshoot as
- From Main Menu, press - From Main Menu, press necessary.
DIAGNOSTICS DIAGNOSTICS - Replace Optical Flow Cell
F12 F12 (PL-10).
F1 F1 - Replace MAM (PL-12).
SET POINT ENTRY SET POINT ENTRY - Replace SPM (PL-10).
- Verify WBC Threshold is 360. - Verify RBC/PLT Threshold is 550. - Replace CPU/DCM (PL-13).
- Ensure WBC Mixing Chamber is - Ensure RBC/PLT Mixing Chamber is
being rinsed and drained properly. being rinsed and drained properly.
System Flags Table
- Verify/adjust WBC Gain (VP-23). - Verify 0 o and 10 o Log Amplifiers (VP-28).
WBC Flags PLT Flags RBC Flags
- Perform WBC OPTI-CAL - Verify 0 o and 10 o Gain (VP-26), (VP-27).
Verification/Adjustment (VP-24). - Replace MAM (PL-12). Var Lym LRI RBC Morph
- Replace MAM (PL-12). - Replace SPM (PL-10). Band URI
- Replace SPM (PL-10). - Replace CPU/DCM (PL-13). IG LURI
Blast
5#7URXEOHVKRRWLQJ IP-22 External Waste Full
IP-22 External Waste Full (Page 1 of 2)
Start
- Ensure Dummy Plug is

System Uses installed
NO
Waste Sensor Cable
- Reseat Dummy Plug
3 2 01 2
?
Problem
YES Total Call
Corrected
- Empty Waste Container
?
- Reseat Waste Sensor Plug
- Clear Fault
NO
- Insert Insulated Tool into

NO Sensor Jack to open
contacts. See Figure
Problem
Corrected
? Total Call 1
YES
5#7URXEOHVKRRWLQJ IP-22 External Waste Full
IP-22 External Waste Full (Page 2 of 2)
- Disconnect Sensor Cable from Waste

Problem Container and Sensor Jack and verify
YES
Corrected infinite resistance between Fluid
? Contacts. Replace as necessary (PL-3.1).
NO Problem
YES Total Call
Corrected
?
Perform in the following order until problem is

corrected:
- Reseat CDM2, J7 and FCM, J2
NO
- Replace Waste Sensor Jack (PL-3.2)
- Replace CDM2 (PL-16)
5#7URXEOHVKRRWLQJ IP-23 Blank Display (Page 1 of 1)
IP-23 Blank Display (Page 1 of 1)
Start
- Connect Monitor to
- Turn Brightness and Contrast Laptop. See Figure
controls fully clockwise. - Verify Laptop
- Open Computer Door to
observe 7-segment LED on - Verify Monitor Power and Power information displays on
CPU/DCM. Cord. Monitor.
- Cycle Analyzer Power and - With Power off, check Video
verify the following: Cable, re-seat all Computer NO
Connectors, re-seat VGA Board.
- Stepper Motors all home.
- Valves activate.
- 7-segment LED increments YES Information
until "E" and "1" are alternately Displays
displayed. ?
Problem
- Analyzer "beeps" after Corrected
Initialization. YES
?
Total Call
YES - Replace VGA Board (PL-
Data Station CRT
17.2). NO
Initialization
OK
?
- Replace Monitor (PL-

NO 17.3).
- Troubleshoot Attach Video Cable

"dead" Analyzer From CRT to Laptop
problem. External Monitor Port
Laptop 32101
Computer
5#7URXEOHVKRRWLQJ IP-24 HSSL Failures/Message Reception Time-Out (Page 1 of 1)
IP-24 HSSL Failures/Message Reception Time-Out (Page 1 of 1)
Start
- In order replace the

- With Power OFF, check following.
and re-seat connectors on - HSSL Cable (PL-17.3)
the CPU/DCM, and Main - CPU/DCM (PL-13)
Computer.
- Serial Link/Keyboard
- Re-seat the HSSL Board. Controller (PL-17.2)
- Check and re-connect the
HSSL Cable. NO
- Reload HSSL Com (VP-67)
Problem
Corrected
?
YES
Total Call
5#7URXEOHVKRRWLQJ IP-25 Hard Drive Errors (Page 1 of 1)
IP-25 Hard Drive Errors (Page 1 of 1)
Start
- Format the Hard Drive (VP-61).

- Install Software (VP-53).
- With Power OFF, re-seat - Load System Setup (VP-65), (VP-
cable between Hard Drive 63).
and Motherboard.
- Re-seat Power Cable on
Hard Drive and CDM. NO
Problem
NO
Corrected
?
Problem
Corrected YES
?
Total Call - Replace Hard Drive (PL-17.1).
YES
Total Call
5#7URXEOHVKRRWLQJ IP-26 Printer Errors
IP-26 Printer Errors (Page 1 of 2)
Start
- Ensure Printer Power is - Check power and power

ON, and Printer is On- cable. Is Printer
line. NO
- Press On-line Button. Working
- Ensure paper is loaded ?
YES
properly. Clear Printer buffer.
NO
YES
SETUP
Printer CUSTOMIZE
On-Line Total Call REPORT
Printer ? STOP PRINTING
On-Line
CONFIRM STOP
? NO
YES - Replace Printer.
Clear Printer buffer.

Printer
SETUP
Reset & NO 1
CUSTOMIZE Print
REPORT ?
STOP PRINTING
CONFIRM STOP YES
Total Call
5#7URXEOHVKRRWLQJ IP-26 Printer Errors
IP-26 Printer Errors (Page 2 of 2)
1
- Connect Laptop Printer to
Printer Connector to
verify problem.
- Try printing with alternate
Printer Cable.
- Replace I/O Board (PL-
NO 17.2).
NO - Replace Motherboard
(PL-17.2).
Laptop
Printer
Prints Prints
OK ?
?
YES
YES
- Replace Printer.
- Replace Printer Cable
(PL-17.3).
5#7URXEOHVKRRWLQJ IP-27 Sample Loader & Aspiration Tower Error Messages
IP-27 Sample Loader & Aspiration Tower Error Messages
Error Message Associated VP(s) Associated Components Parts List(s)

Mix Head stuck in angle position VP-36 Stepper Motor, Home Sensor, SHM 2 PL-18.2, PL-18.1
Mix Head stuck in vertical position VP-36 Stepper Motor, Home Sensor, SHM 2 PL-18.2, PL-18.1
Mix Head not at top position Up/Down Air Cylinder, Sensor, Valve 3, PL-18.1, PL-18.2,
VP-35
SHM 2 PL-18.3
Mix Head stuck at top position Up/Down Air Cylinder, Sensor, Valve 3, PL-18.1, PL-18.2,
VP-35
SHM 2 PL-18.3
Load zone empty detector malfunction VP-.39 Sensor, SHM 2 PL-18.2, PL-18.3
Unload zone full detector malfunction VP-39 Sensor, SHM 2 PL-18.2, PL-18.3
Unload zone nearly full detector Sensor, SHM 2
VP-39 PL-18.2, PL-18.3
malfunction
Tube stuck in position 3 Tube Sensor Board, Rack Advance
VP-38 PL-18.2, PL-18.3
Cylinder, Valve 1, SHM 2
Probe stuck at home position Aspiration Tower, Home Sensor, Needle
VP-31 PL-6.1, PL-8
SHM 1
Probe unable to reach home position Aspiration Tower, Home Sensor, Needle
VP-31 PL-6.1, PL-8
SHM 1
Invalid tube height VP-34 Aspiration Tower, Sensors S1/S2, SHM 1 PL-6.1, PL-8
Unload area hardware malfunction VP-38, VP-39 Unload Side Sensors, SHM 2 PL-18.2, PL-18.3
Tube dropped during mixing Mixer Head, Valve 4, Regulator, SHM 2 PL-18.1, PL-18.2,
VP-37
PL-18.3
Mix Zone Rack position error VP-38 Rack Index Cylinder, Valve 1, SHM 2 PL-18.1, PL-18.3
Unexpected tube in position 4 after Tube Sensor Board, SHM 2
VP-38 PL-18.1, PL-18.2
Rack advance
Tube lost moving from position 3 to Tube Sensor Board, SHM 2
VP-38 PL-18.1, PL-18.2
position 4
Rack barcode read failure VP-40 Rack Barcode Label
5#7URXEOHVKRRWLQJ IP-27 Sample Loader & Aspiration Tower Error Messages
IP-27 Sample Loader & Aspiration Tower Error Messages
Error Message Associated VP(s) Associated Components Parts List(s)

Tube position barcode read failure VP-40 Rack Position Barcode Labels
Barcode reader failure VP-33, VP-11 Barcode Reader, Aspiration Tower, SHM 2 PL-18.2, PL-6.1, PL-18.1
$SSHQGL[#5$#0#&DEOH#&RQQHFWLRQ#'LDJUDP Table of Contents
$SSHQGL[#5$#0#&DEOH#&RQQHFWLRQ#'LDJUDP
Table of Contents
Figure 2A-1 Cable Connection Diagram, SL . . . . . . . . . . . . 2
CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2A - 1
$SSHQGL[#5$#0#&DEOH#&RQQHFWLRQ#'LDJUDP
Figure 2A-1 Cable Connection Diagram, SL

9601320
9520847
FCM
(SOL 11-18) SDM DATA, +5V 9520837
J3 9520783
SDM 1 J9 CPU
SOL 9600930 SOL START SW 9601820
NC NC
1 J1 J6 J6 J6
2 J2 J7 7 J9 9520825 9520825 J13 9520864 DATA STATION *S/L SPM
J6 J1 J5
3 J3 J8 8 9520828 NC 9601830
4 J4 J9 J3 SPEAKER & J12
9520830 9520830 STATUS BD 9600910 IN-LINE 9520865 J3 9520865
5 J5 J10 J1 9520870 J14
9520858 J26 REAGENT 9520500
J2 SP SENSOR BD J3 J4
9520838 9520851 DAC1 9520846 J1 9520846
P/O J7 J22 J8 J11
(SOL 21-28) SDM/CDM 9520852 ADC1 9520505 DAC3
J25 J7 J10 J5
SDM 2 9520850 ADC3
J12 J7
9600930 9520826 NC 9520892 9520848
J2 J16 J1 J6
1 J1 J6 6 NC NC 9520841
2 J2 J7 7 J15 J2 J2
VAC/PRES ULTRA SONIC 9520893
3 J3 J8 8 SOLENOID 9601220 9520820 SAMPLE DET J17
J4 J9 J1 J14 NC
4 1 J1 J13
9520858 9601161 J11 J3
5 J5 J10 MAM
2 J2 2nd SAMPLE DET.
9520841 9520841 J20 9520841 9520841 9601950
J4 MUX DAC CTRL. J2
EXT. CPU BUS

3 J15
+5V. +/-15V
J3 9520849 9520849 J19 9520849 9520506 ADC2
4 J4 J17 J16 J9 J4 J15
(SOL 31-38) 9520829 9520829 PERIPHERAL BUS
5 J5 J12 J21 J1
9520831 9520831 9520865
J13 J23 DILUENT J3
9520832 9520832 9520839 NC 9520853 9601000
1 J51 J56 6 J14 J24 J10 P OVER PRESS J5 J10 J1
J52 9520861 SENSOR NC PREAMP 0 DEG (CH1)
2 J57 7 12"VAC OVFL J4 J6
3 J53 J58 8 9520528 9520866 9340008 NC 9520854 9601000
J10 J5 9212057 J7 J12 J1
4 J54 8"VAC OVFL 9520862 9520385 LINEAR P/S PREAMP 10 DEG (CH2)
J18 HGB NC
J11 J8 J11 PMT PREAMP
9520833 TOP
TEST CONN. J16 9601010
P/O 9520878 NC 9520855 J1
J6 J18 J2 J6 J13 J2 PMT (CH3)
SDM/CDM 9520879
J3
9520834 SHEAR VALVE DR. NC 9520001
9600900 J1 J7 9520856 J1
(SOL 41) J1 FAN J14
PRES PUMP J2 PMT (CH4)
1 J61 J2 PUMP 9520878 NC 9520620 J3
VAC PUMP J4 J3 J8
J68 J3 RELAY FAN PMT PREAMP
J67 9601200 9212064 9601010
9520862 9520002
J2 LASER LASER P/S J4 J13
9520869 PWR SW
CDM 2 J3 MPM
} 9600920
9601080 EX WASTE 9520896
9520857 J501
9520879 9520847 MPM SERIAL LINK
J4 J7 J1 MOTOR
(SOL 51-58) 9520826 DIL SENS J1 J2 B HGB LYSE SYR.
J6 J8 MOTOR
SDM 5
9520859 NC 9520835 TO REAGENT 9520845
J1 J9 RESERVOIRS J3 J1 J2 A CORE SYR.
9600930 9520860 SHEATH SENS 9520844
J2 J10 BACK J4 MOTOR
1 J1 J6 6 NC 9520836 9520869 J3 9520155
2 J2 J7 7 J5 J5 J1 J2 C WBC LYSE SYR.
NC 9520866 12V, 24V UNREG. 9520866 9520154
3 J3 J8 NC J11 J3 J1 J6
J4 J9 12V, 24V UNREG. 9520828 MOTOR
4 9520842
9520859 J2 J7 J1 J2 E PROBE
5 J5 J10 MOTOR
PDM J8 MOTOR
NC 9520563 J1 J2 D RBC/HGB DIL SYR.
9601980 J5 J9 J1 J2 G SAMPLE PUMP
(SOL 91-98) 9520829 CHOPPER DRIVER
(SOL 61-68) J8 J2 SENSOR & CHOPPER DRIVER 3X 9600420
SDM 9 9520896 9520782 NC
J1 J10 3X 9600420
SDM 6 9600930 J4
SPARE NC
9600930 1 J1 J6 6 J7 J11
1 J1 J6 6 2 J2 J7 7 9520782 +/-15V NC
J9 J12 9601910
2 J2 J7 7 3 J3 J8 8 9520865 NC SHM(2)
J4 J13
3 J3 J8 8 4 J4 J9 NC
9520861 9520543 9520543 J14 J1 TO SL VAL-1
4 J4 J9 5 J5 J10 J6
5 J5 J10 9320860 HARD DISK
9520897
J2 TO SL VAL-2
J10
J3 TO SL VAL-3
9520898 9520895
FLOPPY J11 J4 TO SL VAL-4
9520895 9601910
MDM 9601940 J13 SHM(1) J5 TO SL VAL-5
9520796 9520884
J2 9520894 J1 TO TOWER SOL. J10 TO SL MIXER SENS.
9520795 J12 9520872 TO TOWER SENS.
J1 J10
J6 J3 9520894
MOTOR J12 J12
9520789 9520612 TO PC MOTHERBOARD 9520890
J3 J2 9520883
J13 TO TOWER MOTOR J13 TO SL MIXER MOTOR
9520789 J5 9520882 9520888
9520871 2 J14 TO TOWER DC-MOTOR J15 TO BAR CODE READER
J5 9520780 9520887
J9 TO COVER SENSOR J9 TO SL RACK SENSORS
9520892 9212106
NC J7 32054 J16 J11 TO SL TUBE SENS ASSY
9520871 1 ASPIRATION 9520893 J16
J4 TOWER
Y-VALVE ASSY CONTROLER SAMPLE LOADER
BOTTOM VIEW CONTROLLER
4PLCS
SDM1-4.....FLOW PANEL 1 FLOW PANEL (X2) 9520255 ASY3A
*S/L=SERIAL LINK PCS PANEL
SDM5,6.....REAGENT PANEL CHAIN CABLE
SOLENOID CHAIN CABLE LASER PANEL
CONNECT THE DOOR AND PLATE TO CHASSIS GND.
CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2A - 2
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Table of Contents
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV
Table of Contents
Figure 2B-1 ADC Block Diagram . . . . . . . . . . . . . . . . . . . . . 2

Figure 2B-2 ADC Block Diagram . . . . . . . . . . . . . . . . . . . . . 3
Figure 2B-3 DAC Block Diagram . . . . . . . . . . . . . . . . . . . . . 4
Figure 2B-4 Measurement & Subsystem Block Diagram . . 5
Figure 2B-5 Solenoid Driver Modules 1, 2 & SDM/CDM . . . 6
Figure 2B-6 Solenoid Driver Modules 5, 6, 9. . . . . . . . . . . . 7
Figure 2B-7 Motor Driver Block Diagram. . . . . . . . . . . . . . . 8
Figure 2B-8 CS Sensor Interface Block Diagram . . . . . . . . 9
Figure 2B-9 S/L Sensor Interface Block Diagram . . . . . . . 10
Figure 2B-10 Vacuum/Pressure Block Diagram . . . . . . . . . 11
Figure 2B-11 Power Distribution Block Diagram . . . . . . . . . 12
Figure 2B-12 Computer Block Diagram. . . . . . . . . . . . . . . . 13
Figure 2B-13 Measurement Block Diagram. . . . . . . . . . . . . 14
Figure 2B-14 Solenoid Control Block Diagram . . . . . . . . . . 15
Figure 2B-15 Motor Control Block Diagram. . . . . . . . . . . . . 16
Figure 2B-16 Sensor Block Diagram . . . . . . . . . . . . . . . . . . 17
Figure 2B-17 Power Distribution Block Diagram . . . . . . . . . 18
Figure 2B-18 Normal 10, 90 and 0, 90D Scattergrams . . . . 19
Figure 2B-19 Normal 10, 90 and 0, 90D Scattergrams . . . . 20
Figure 2B-20 Normal PLT and RBC Histograms . . . . . . . . . 21
CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2B - 1
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-1 ADC Block Diagram
Figure 2B-1 Figure 2B-1ADC Block Diagram
Display
PC Module
VPM
High Speed
Serial Link
Tag Bus
SPM
High
Speed
External
CPU
FCM
Bus
MAM
CPU/DCM MUX DAC Control Bus
MPM
Serial
Bus
MPM
Peripheral Bus
Serial RS-485 Bus To SHM1

(Aspiration Tower Control) 32043
Serial RS-485 Bus To SHM2

(Sample Loader Control)
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-2 ADC Block Diagram
Figure 2B-2 ADC Block Diagram
Display
PC Module
High Speed
Serial Link
ADC1
ADC3
A/D MUX
ADC2
Tag Bus
CPU/DCM
ADC4
MAM SPM FCM VPM
32044
MUX DAC Control
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-3 DAC Block Diagram
Figure 2B-3 DAC Block Diagram
Display
PC Module
High Speed
Serial Link
DAC1
D/A DAC2
DAC3
CPU/DCM
DAC4
MAM SPM FCM VPM
MUX DAC Control 32045
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-4 Measurement & Subsystem
Figure 2B-4 Measurement & Subsystem Block Diagram
Display
0 o Lin
PD 0 o PAM
10 o Lin PC Module
90 o Lin
PD 10 o PAM
90 o D Lin High Speed
Serial Link
MAM 0 o Log SPM
PMT 90 o PAM
10 o Log
VDYN Analog Out
Tag Bus
PMT 90 o D PAM
VDYN CPU/DCM
External CPU Bus

Peripheral Bus
LED Current HGB Voltage

HGB
Flow HGB Signal FCM
Cell 32046
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-5 Solen Dr Mod 1, 2 & SDM/CDM
Figure 2B-5 Solenoid Driver Modules 1, 2 & SDM/CDM

Solenoid Data Bus
3-1 WC2 Vacuum Supply (5" Hg)

3-2 WC2 Pressure (12 psi)
3-3 RBC Diluent Syringe Supply
3-4 CS Probe Diluent Flush (NC)
CLK 3-6 CS Vent Trap Drain (WC3)
SDM/CDM 3-7 CS Probe Drain (WC4)
(CDM1) 3-8 CS Vent Trap Vent
4-1 NOC Sample Staging
4-7 ON/OFF Motor

DC Y-Valve
4-8 CW/CCW Drive
Module Motor
1-1 Shear Valve Diluent Flush

1-2 Sample Aspiration Vacuum
FCM 1-3 Shear Valve Drain (WC4)
1-4 Open Sample Probe Drain (WC4)
CLK S D M 1
1-5 Open Sample Probe Diluent Flush (NC)
1-6 Spare
1-7 WC4 Vacuum Supply (12" Hg)
1-8 WC4 Pressure Supply (12 psi)
Peripheral Bus
2-1 RBC Mixing Chamber Drain

2-2 Sample Injection Syringe Sheath Supply
CPU/
2-3 Main WBC Lyse Supply (NC)
DCM
2-4 HGB Lyse Syringe Output
SDM 2
2-5 WBC Lyse Syringe Output
CLK
2-6 Diluent Syringe HGB Dilution
2-7 Diluent Syringe RBC Dilution
2-8 Main HGB Lyse Supply (NC)
1 12 psi Accumulator Vent

2 9 psi Pressure Supply
VPM 3 5 psi Pressure Supply

4 12" Hg Accumulator Vent
5 Vacuum #2 Vacuum Supply
32047
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-6 Solenoid Dr Modules 5, 6, 9
Figure 2B-6 Solenoid Driver Modules 5, 6, 9
Solenoid Data Bus
5-1 HGB Flow Cell Vent

5-2 Staging Pump Input
5-3 WBC Cup Vent
5-4 RBC/PLT Sample Staging
CLK SDM 5
FCM 5-5 WBC Sample Staging
CDM 2
5-6 Optical Flow Cell Output
5-7 Optical Flow Cell Drain
5-8 Spare
Peripheral
6-1 Sheath Reservoir Vacuum (12" Hg)
Bus
6-2 Diluent Reservoir Vacuum (12" Hg)
6-3 Diluent Reservoir Pressure (5 psi)
CPU/ 6-4 Sheath Reservoir Pressure (9 psi)
DCM SDM 6
CLK 6-5 Sheath Reservoir Output (NC)
6-6 Diluent Reservoir Output (NC)
6-8 WC1 Vent
9-1 Optical Flow Cell Sheath Flow

9-2 WBC Cup Drain (WC3)
9-3 HGB Flow Cell Drain (WC3)
CLK 9-4 HGB Flow Cell Diluent Flush
SDM 9
9-5 RBC Mixing Chamber Diluent Flush
9-6 WBC Mixing Chamber Diluent Flush
9-7 WC4 Vacuum (12" Hg)
32048
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-7 Motor Driver Block Diagram
Figure 2B-7 Motor Driver Block Diagram
A Aspiration Probe Up/Down

SHM1
DC Barcode Spin
RS-485
Serial Driver Motor
Bus A A Sample Delivery Syringe
B B HGB Lyse Syringe

MPM
Serial C C WBC Lyse Syringe
Bus
MPM
CPU/DCM D D RBC Diluent Syringe
E E Open Probe Up/Down
G G Sample Staging Pump
Peripheral Bus AC Vacuum Pump

VPM
AC Pressure Pump
Limit Switch (CW)

FCM Shear
Valve DC Shear Valve Motor
Driver
Limit Switch (CCW)
4-7 ON/OFF
Motor
DC Y-Valve
SCM/CDM Drive
4-8 CW/CCW Motor
Module
32049
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-8 CS Sensor Interface Block
Figure 2B-8 CS Sensor Interface Block Diagram
Display
Vacuum Accumulator #1 Wet
VPM
PC Module Vacuum Accumulator #2 Wet
High Speed
Serial Link Diluent Empty
WBC Lyse Empty

HGB Lyse Empty
CPU/DCM
Peripheral Bus
External Waste Full
CDM2 Diluent Reservior Full

RS-485
Serial Sheath Reservoir Full
Bus
FCM
Tube Height (S1)
Shear Limit Switch (CCW)
Tube Height (S2) Valve
Driver Limit Switch (CW)
SHM1 GS1 Home
Door Closed Interface Ultrasonic Short Sample
Board
Sample Cover
Y-Valve Closed Mode Sensor

Interface MDM
Short Sample
Board
Y-Valve Open Mode Sensor
32050
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-9 S/L Sensor Interface Block
Figure 2B-9 S/L Sensor Interface Block Diagram
Third Tube Sensor

Typical Tube Sensor
Fourth Tube Sensor
J11
25 KHZ LED Drive
RS-485 Bus
To CPU/DCM Tube
J16
SHM2 Load Side Empty Sensor

Power
From PDM J12
J9 U. S. Fourth Rack Sensor
U. S. Fifth Rack Sensor
Mixer Home Sensor (Up)

J10
Mixer Head Home Sensor
32105
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-10 Vacuum/Pressure Block
Figure 2B-10 Vacuum/Pressure Block Diagram
Vacuum/Pressure Module 32051
ADC4
Analog Multiplexer
DAC4 Threshold Setpoints Multiplexer

&
Sample and Hold Circuitry P1 P2 P3 V1 V2
P1 P2 P3 V1 V2
SPP1 SPP2 SPP3 SPV1 SPV2
Pres #1 Pres #2 Pres #3 Vac #1 Vac #2

Regulator Regulator Regulator Regulator Regulator
12 psi 9 psi 5 psi 12" Hg (Variable)
V3
V5
Relay PCB Relay PCB
V2
Pressure Vacuum
Pump Pump Vac #2
Pres #1 Pres #2 Pres #3 Vac #1
Accumulator
Accumulator Accumulator Accumulator Accumulator
5" Hg
12 psi 9 psi 5 psi 12" Hg
(Varies)
V1 V4
Vent Vent
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-11 Power Distribution Block
Figure 2B-11 Power Distribution Block Diagram
AC Line
Pow er Sw itch
MAIN POWER SUPPLY PDM J2 + 5, GND-PC MB
+ 12, -12, GND, PF-PC MB

J13 J3
+ 5, -15, GND-MAM, SPM, CPU
J4
+ 5V + 5, + 24, GND-MDM
J5
+ 12V
+ 5, + 12, -12, + 24-MPM
J5 -12V J1 J6
Filter + 24V Spare
(CORCOM) J7
GND + 5, + 15, -15, GND-VPM, FCM
J8
Fuse + 15V + 5. + 12, GND-Hard Drive
J10
J4 -15V + 5. + 12, GND-Floppy Drive
J9
(Rear) GND J11
+ 5, + 15, -15, GND-SHM1
AC Voltage J12
Selector + 5, + 15, -15, GND-SHM2
J13
Transformer
J4 115VA C + 2400VDC
LASER P/S LASER
(Top)
115VAC, Rear Fan
J7
115VAC, Side Fan
J8
115VAC, PRM
J2
32052
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-12 Computer Block Diagram
Figure 2B-12 Computer Block Diagram
H ar d D i s k Flo p p y D is k
D r iv e D ri v e
C PU / D C M
K e y b o ard
HSSL
Pa r a lle l/
M o t h er b o a rd
S e ria l Po rt s
H SS L
C o n t r o ller
Pa r a lle l/
S e ria l Po rt s
Bu s
V id e o D is p la y
C o n t r o ller 32053
M e m b r a n e K e y b o a rd
Po w er V id e o D is p la y
C RT
S u p p ly M o d u le
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-13 Measurement Block Diagram
Figure 2B-13 Measurement Block Diagram
MAM SPM
TP1 J1 TP3
J10 J15 J6
6 CH 1 Lin CH 1 Lin
Amplifiers 4 Lin Amplifiers 1 1
CH 2 Lin CH 2 Lin J7
3 3
CH 3 Lin CH 3 Lin Peak
TP2 5 5 Amplifier 1 ADC3
TP8 CH 4 Lin CH 4 Lin Hold MUX
7 7 Circuits 2 To CPU/DCM
CH 1 Log CH 1 Log
0 Photodiode Preamp 2 9 9 3
Log Amplifiers CH 2 Log CH 2 Log
11 11
AGND
AGND AGND
TP1 J1 TP4
J12
Amplifiers 6 4 Lin Amplifiers
FCM
TP2
TP9 J18
10 Photodiode Preamp 2 HGB Voltage Amplifier
TP7 TP1 Log Amplifiers 1
J25
HGB 2
AGND
Flow 3 MUX Amplifier 1 ADC1
AGND
Cell 4 2 To CPU/DCM
LED Current
5 3
AGND
J2 AGND
TP2 TP5 AGND
J13
Amplifiers Lin Amplifiers J24
4 4
1 ADC4
TP3 Amplifier From
PMT
2 VPM
VDYN/-100
8 8
TP1
AGND
90 PMT Preamp
2 32055
AGND
J2
TP2 TP6
J14
Amplifiers 4 4 Lin Amplifiers
TP3
PMT
VDYN/-100
8 8 TP2
TP1
AGND 90 D PMT Preamp
2 +10V Reference
AGND
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-14 Solenoid Control Block
Figure 2B-14 Solenoid Control Block Diagram
SDM/CDM
CDM Connectors
Solenoid Data/+5V/5V Ret.
J3 J1
J51 3-1 WC4 Vacuum Supply (12" Hg) +24V UR
5 1
J52 3-2 WC4 Pressure (12 psi) From 4 +12V UR
3-3 RBC Diluent Syringe Supply 2
J53 P. S. 3 UR RET 3 To
J54 3-4 CS Probe Diluent Flush (NC)
3-6 CS Vent Trap Drain (WC3) 1 5 SDMA
J56
3-7 CS Probe Drain (WC4) 6
J9 J57 J6 7
J58 3-8 CS Vent Trap Vent
J61 On CLK A
4-1 NOC Sample Staging 1
J3 Hi CLK A
CLK 2 J2
MDM On CLK B
J1 J6 SDM5 3
Hi CLK B
Y From 4 1
J68 J2 DC J1 5-1 HGB Flow Cell Vent On CLK C
ON/OFF J6 Valve FCM 5 2
J67 J1 Motor J9 J2 5-2 Staging Pump Input Hi CLK C
FCM CW/CCW 6 On CLK D 3 To
CLK J3 5-3 WBC Cup Vent
CLK J1 7 5 SDMB
J2 J4 5-4 RBC/PLT Sample Staging Hi CLK D
CLK 5-5 WBC Sample Staging 8 6
J4 CLK J5 Reset
J2 CDM2 9 7
J19 J6 5-6 Optical Flow Cell Output
J1 J10 J7 5-7 Optical Flow Cell Drain
J4
J2 J8 5-8 Spare
Peripheral Bus J1 1-1 Shear Valve Diluent Flush
J9 J2 1-2 Sample Aspiration Vacuum 1
1-3 Shear Valve Drain (WC4) 2
J3
3 To
J4 1-4 Open Sample Probe Drain (WC4) SDM6
5 SDMC
J5 1-5 Open Sample Probe Diluent Flush (NC)
J16 J6 J1 6-1 Sheath Reservoir Vacuum (12" Hg) 6
CPU/DCM J6 1-6 Spare
J9 J2 6-2 Diluent Reservoir Vacuum (12" Hg) 7
J17 J1 J10 J7 1-7 WC2 Vacuum Supply (4" Hg)
J3 6-3 Diluent Reservoir Pressure (5 psi)
J8 1-8 WC2 Pressure Supply (12 psi)
J4 6-4 Sheath Reservoir Pressure (9 psi) J5
SDM1 J5 6-5 Sheath Reservoir Output (NC)
6-6 Diluent Reservoir Output (NC) 1
SDM2 J6
6-7 WC1 Pressure (12 psi) 2
J10 J7 To
2-1 RBC Mixing Chamber Drain 6-8 WC1 Vent 3
J1 J8
J9 J2 2-2 Sample Injection Syringe Sheath Supply 5 SDMD
2-3 Main WBC Lyse Supply (NC) 6
J3
2-4 HGB Lyse Syringe Output 7
J4
SDM9
J5 2-5 WBC Lyse Syringe Output
J6 2-6 Diluent Syringe HGB Dilution J1 9-1 Optical Flow Cell Sheath Flow
J10 J7 2-7 Diluent Syringe RBC Dilution J9 J2 9-2 WBC Mixing Chamber Drain (WC3)
J8 2-8 Main HGB Lyse Supply (NC) J3 9-3 HGB Flow Cell Drain (WC3)
J4 9-4 HGB Flow Cell Diluent Flush
J5 9-5 RBC Mixing Chamber Diluent Flush
J6 9-6 WBC Mixing Chamber Diluent Flush 32056
J10 J7 9-7 WC3 Vacuum (12" Hg)
J8 9-8 WC3 Pressure (12 psi)
VPM
J1 1 12 psi Accumulator Vent FCM Connectors SDM Connectors & Driver Outputs
J17 J9
J2 2 9 psi Pressure Supply J1 J3
PD0 +24VUR +12VUR
J3 3 5 psi Pressure Supply On CLK 1 PD0 1
Hi CLK 1 1 1 PD1
J4 4 12" Hg Accumulator Vent PD1 2
5 Vacuum #2 Vacuum Supply On CLK 2 2 2 PD2
J5 PD2 3
3 3 PD3
Hi CLK 2 PD3 4
4 4 PD4
Aspiration Tower SHM Solenoid Connector On CLK 3 To PD4 To 5
SHM1 5 5 PD5 Hi/Lo
Hi CLK 3 CDM1 PD5 SDMs 6 Driver
RS-485 Bus GS2 Stop +24V On CLK 4 6 6 PD6
J16 J1 7
PD6
7
7
PD7 TP1
J2 Door Lock Hi CLK 4 PD7 8
J1-J8 8 8 +5V
J3 Spare Reset +5V 9
9 9
J4 Spare 10
1 15 10
J5 Spare
J6 Spare 2
J1-J8
J7 Spare DGND
AGND DGND
J8 Spare 1
J10 Solenoid Drive
On/Off J2 J4 +24VUR On/Off
Driver TP2 Driver 2
SHM2 Sample Loader On CLK 5 +24VUR 1 +12VUR
Hi CLK 5 1 1
RS-485 Bus +12VUR 2 UR RET
J1 V1 Rack Advance 2 2
J16 On CLK 6 UR RET 3
J2 V2 Load Side Cross Transfer 3 To 3 To On CLK
Hi CLK 6 On CLK 9 5 Hi CLK
J3 V3 Mixer Lift
Reset 4 CDM2 5 SDM9
Hi CLK 9 6
J4 V4 Mixer Bladders 9 6 Reset
Reset 7
J5 V5 Unload Side Cross Transfer 15 7
J6 Spare
J7 Spare
J8 Spare AGND
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-15 Motor Control Block Diagram
Figure 2B-15 Motor Control Block Diagram

MPM Stepper Motor Control
Aspiration Tower Motor Control Sample Loader Motor Control

MPM Serial Link A
J6 JI J3 JI CDB J2 Delivery Syringe GS1 Stepper Motor Mixer Stepper Motor
CPU/DCM
JI CDB J2 B HGB Lyse Syringe PH0
J4 1 PH0 1
Motor Motor
J5 JI CDB J2 C WBC Lyse Syringe PH0
2 PH0 2
MPM J13
J6 JI CDB J2 D RBC/HGB Diluent Syringe SHM2 J13
SHM1 PH1
J7 JI CDB J2 E Open Probe PH1 4
4
PH1
J8 5 PH1 5
J9 JI CDB J2 G Sample Staging Pump Barcode Spin Motor

+24V 1
J8
Driver 2 DC
MPM Motor Control Block Diagram (Motor 0) Typical MPM Motor Control Output Connector
J3 J3-J14
Discharge FB+
Microprocessor Ramping 8 1
Discharge TO +24V
& A/D
Complete FB- CDB 2
Digital Control Converter 9 +5V
3 Shear Valve Motor Control
M0 I0
4 +5V
M0 I1 1 1 + Drive
5 TO 1
3 DGND 3 Shear
Digital Control Bus M0 PH0 CDB
6 FCM J8 J2 Valve
J1 On/Off J4
J3 M0 PH1 4 4 Driver
MPM J3
M0 I0 7 CW/CCW Module - Drive
Serial M0 M0 PH0 6 M0 4 M1-M11 5 5 3 DC
FB+
Link Phase TO Current TO Control 8
Control M0 PH1 CDB Control M0 I1 CDB Latches FB-
Latch 7 Latch 5 J4-J14 9 Shear Valve Motor
10
Y-Valve Motor Control

CDB Block Diagram
Chopper Driver Board
J1 J2 On/Off Y-Valve Motor
J68 2 2 J2 1
PH0
6 Stepper MA
PH0 1 SDM/CDM MDM J14
I0 Motor CW/CCW 2 DC
4 Motor J67 2 2 J1
Phase 0 PH0 2
I1 Driver MB
5
PH1
7 Stepper PH1
TO MA 4
MPM +24V Motor Vacuum & Pressure Pump Control
2 Phase 1 PH1
Driver MB 5 Pump Relay Board
3 +5V F1
Line
TP1 1
3+ AC In 1 Neutral J4 115VAC In
GND 2
1 K1
FB+ 4- AC Out 2
Winding +5V Vacuum Pump
FB+ Voltage 1 1
8 J1 1
Feedback Vac. Pump On
VPM J18 3 3 J3
FB- (Diagnostics) 2 AC
9 FB- Pres. Pump On
4 4
3+ AC In 1
K2
1
4- AC Out 2 J2
32057 2 AC
Pressure Pump
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-16 Sensor Block Diagram
Figure 2B-16 Sensor Block Diagram

Typical FCM & VPM Fluid Sensor Input Reagent Empty In-Line Sensors
IFSB Aspiration Tower Sensors Typical SHM Optical Sensor Input
Electrode +
J1
E5 GS1 Home Sensor 3
FLUID E1 Diluent Empty 1 1 +5V
LINE J9-10
E6 Tube Height Sensor (S2) 6 J10
E2 WBC Lyse Empty 2 2 1
Electrode -
J26 SHM1 2
E8 Tube Height Sensor (S1) 9 3 In
E4 HGB Lyse Empty 4 4 4
5
GND Cover Sensor 3 Typical SHM Magnetic Sensor Input 6 In
6 6 7
J9 8
Door Sensor 6 9 In
10
11
Reagent & Waste Level Sensors CDM2 12 In
Sample Loader Sensors
External Waste Full 1 J7 10 10
Load Side Empty Sensor 3 DGND
Diluent Reservoir Level 1 J8 11 11 Typical SHM Tube Sensor Input

U. S. Fifth Rack Sensor 6 J9
J6 J2
Sheath Reservoir Level 1 J10 13 13
U. S. Fouth Rack Sensor 9 +5V
GND SHM2 J11
15 15 25KHZ LED Drive
Mixer Head Home Sensor 3 1
J10 2
3 In
Mixer Lifter Up Sensor 6 4
Second Sample Sensor Board 5 25KHZ LED Drive
FCM 6
Fourth Tube Sensor 4 7
+5V
J11 8 In
TP4 9
TP2 Third Tube Sensor 8 10
1 +5V 1 11
12
Ref 2 2 J14
J1 3 AGND
3 Vacuum Accumulator Sensors AGND
Vacuum #1 Wet 1 J1
AGND VPM Y-Valve Sensors
Vacuum #2 Wet 1 J2
Closed Mode Sensor 2 J4
Ultrasonic Short Sample Sensor Board MDM
+5V
Open Mode Sensor 2 J5
1 1
J1 2 2 J11
AGND Typical MDM Optical Sensor Input
3 3
+5V
Limit Sensor Baord Shear Valve Driver Module J4-J5
+5V +5V 1
2 2 1 1 2 In
J1 3
Valve CCW DGND
3 3 3 3
J1 TP1 J2 J8
Valve CW Valve CW
4 4 6 6
DGND Valve CCW AGND
6 6 7 7
RBC Overpressure Sensor
NO +5V
2
GND J10
3
32058
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-17 Power Distribution Block
Figure 2B-17 Power Distribution Block Diagram

Power Distribution Module Power Connectors Main Power Supply Power Connectors
J1 CDM 1 & 2 Power Connectors
J501 BACK
PWR Fail J3 J1
1 PWR Fail
2 1 +24V UR
-12V 3 2 5 1 To
+12V 4 Input -12V From +12V UR
3 4 2 SDMA
+24V 5 From To +12V P. S. UR RET
J8 P.S. 4 J1/J2 3 3
GND 1 6 PDM-J1 5 +24V
+5V GND 1 7 J501 GND 1 1
1 6 GND 1
2 GND 2 +5V 8 7
To +5V 9 +5V
FCM-J21 3 +15V TP8 8 +5V
VPM-J12 4 GND 2 9
5 +15V TP2 J2
6 -15V
GND 1 J2
-12V 1 J1
GND 1 2
J9 UR RET 1
To PC 1 To
Input 1
-15V
TP7 +5V 3 4 Motherboard
To 3 2 SDMB
From 2 GND 2 +5V 5 CDM2-J3 4 +12V UR 3
P.S. 3 KEY
+24V +5V 6 FCM-J5 5 +24V UR
4 +15V
J4
J3
PWR Fail J2
1 UR RET
+5V 2 1 J4
+12V 3 To PC To 3
J10
+12V
-12V 4 Motherboard SDM/CDM-J3 4 +12V UR 1
To GND 1 5 +24V UR To
1 GND 2 5 2
PC 2 GND 2 GND 1 6 SDMC
Hard 3 +5V 3
Disk 4 TP1 J3
J4 To
+5V Shear +24V UR
J11 TP5 GND 1 1 1
+5V 2 Valve 2 RET
To +12V To
1 GND 2 3 MAM-J3 Driver-J3
PC 2 GND 2 GND 1 4 J5
Floppy 3 GND 2 GND 1 5 SPM-J3
+5V CPU/DCM-J14 J4 1
Disk 4 6 To
GND 1 7 To 1 -15V 2 SDMD
-15V 8 2 GND 2
J12 3
+24V
PDM-J9 3 +15V
1 J5
2 +5V +24V 1
3 To FCM Power Connectors
To 4 +15V +5V 2 MDM-J3
SHM 1-J12 5 -15V GND 1 3
J3
6 GND 2 TP6 J2 TOP
7 GND 2 J6 115VAC
To 9 +5V
8 +5V
To 1 SDMs-J9 10 GND
-12V
1 PRM-J4 2 Neutral
+12V
J13 2 J4
+24V 3
+24V GND 1 To +24V UR
1 GND 1 4 J4 To 1
2 +5V GND 1 5 MPM-J2 To
115VAC SDM9-J10 2 +12V UR
3 6 Laser 1 Neutral 3 UR RET
To 4 +15V TP3 7 Power 2
SHM 2-J12 -15V +5V
5 GND 2 8 Supply To J8
6 -15V
7 J7 J7
Shear 1 +5V
GND 2 Valve 3 GND
8 To 115VAC
TP4 -12V 1 1 Driver-J2
+12V 2 Rear 2 Neutral To J11
3 Fan
+12V GND 1 4 Spare Ultrasonic 1 +5V
GND 1 5
J8 Sample 3 GND
GND 1 6 Sensor-J3
Typical MPM Motor Control Output Connector To 115VAC
7 Side 1 Neutral To J14
J3-J14 +5V 8 2
Fan Second 1 +5V
1 Sample 3 GND
+24V Sensor-J1
2
+5V
3
M0 I0
4
Note: Pin configurations on the assemblies that are not 32059
M0 I1
5 TO shown are the same as those shown.
M0 PH0 CDB
6
M0 PH1
7
FB+
8
FB-
9
10
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-18 Normal 10,90 & 0,90D S'grams
Figure 2B-18 Normal 10, 90 and 0, 90D Scattergrams
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-19 Normal 10,90 & 0,90D S'grams
Figure 2B-19 Normal 10, 90 and 0, 90D Scattergrams
3 rd Cut 4 th Cut
M
E
E
0 Size
90 Dep
N L B
B
L
M
0 10 Complexity 32103
B - Basophil L - Lymphocyte N - Neutrophil

E - Eosinophil M - Monocyte
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-20 Norm PLT & RBC Histograms
Figure 2B-20 Normal PLT and RBC Histograms
RBC/PLT PLT
PLT RBC 1. Curve Starts to the Right of the Lower Threshold.
2. Single Peak
32104
3. Good Distance From Lower Threshold at Peak
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Table of Contents
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV
Table of Contents
Figure 2C-1 Flow Diagram, Complete . . . . . . . . . . . . . . . . . 2

Figure 2C-2 Sample Loader Flow Diagram . . . . . . . . . . . . . 3
Figure 2C-3 Vacuum/Pressure Flow Diagram . . . . . . . . . . . 4
Figure 2C-4 Reagent Flow Diagram . . . . . . . . . . . . . . . . . . 5
Figure 2C-5 Sample Aspiration Flow Diagram. . . . . . . . . . . 6
Figure 2C-6 Dilution and Mixing Flow Diagram . . . . . . . . . . 7
Figure 2C-7 Sample Staging Flow Diagram . . . . . . . . . . . . 8
Figure 2C-8 Sample Delivery Flow Diagram . . . . . . . . . . . . 9
Figure 2C-9 Waste Flow Diagram . . . . . . . . . . . . . . . . . . . 10
Figure 2C-10 Vent Flow Diagram . . . . . . . . . . . . . . . . . . . . 11
Service Commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2C - 1
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Figure 2C-1 Flow Diagram, Complete
Figure 2C-1 Flow Diagram, Complete
REAGENT & WASTE PANEL BLOOD/OUT, FARSIDE

WBC/OUT
HGB LYSE WBC DIL/IN, FARSIDE
HGB/OUT
TO AIR DILUENT
BLOOD/IN
WBC LYSE
MUFFLER WASTE RBC DIL/IN, FARSIDE
EMPTY 62 63 61 64 HGB DIL/IN RBC/OUT
PRES SENSORS
WBC LYSE
PUMP
FRONT VALVE REAR VALVE
TO AIR
TO AIR
FILTER 1
DILUENT SHEATH HGB LYSE
RESERVOIR RESERVOIR
23 28
PS1
TOP FILTER (SMALL)
HGB LYSE (BLU)
SENSOR
ACC 1 WBC LYSE (VIO)
PRES 1 91 WBC
66 65
(12 psi) TO AIR FLOW
DILUENT (RED) CELL
FILTER 94 96
(LARGE) DILUENT (RED) 1 2 3 4 5
2
56
54 57
WBC OUT 53
PS2
SAMPLE WBC LYSE 52
SENSOR
ACC 2 SENSOR 2
95 HGB OUT 51 WBC
PRES 2 WBC M. C. PERIS-
20 uL 55
(9 psi) MOTOR-X HGB TALTIC
VENT HGB DIL
12 uL
BLOOD RBC 22 PUMP
ACC SAMPLE 1.67 uL
VALVE MOTOR-G
3 RBC RBC DIL
MIXING RBC OUT TRAP
CHAMBER ULTRASONIC HGB 500 uL
SENSOR FLOW SAMPLE
11 12 CELL INJECTION
PS3 SY1
41 92
SENSOR 13 MOTOR-A
VENT
ACC 3
PRES 3 TRAP TRAP
(5 psi) 93 67 68
Y-VALVE PRESSURE
47,48 TO AIR SWITCH
TO SAMPLE LOADER 17
TO AIR
(SL MODEL ONLY) 21
MUFFLER 14 OPEN 27 26
SAMPLE 25 24
VAC PROBE
15 33
PUMP 38
VAC 1 (GRN) WC-1
MOTOR-E WC-2
10 mL 2.5 mL 2.5 mL
34 RBC WBC HGB
DIL LYSE LYSE
PS4 TO AIR WC-4 VENT SYRINGE SYRINGE SY2
WC-3 TRAP SY4 SY3
SENSOR
ACC 4 37 MOTOR-D MOTOR-C MOTOR-B 18
VAC 1 4 36
(12" Hg) CLOSED
MODE
31 32 97 98 PROBE
DRAIN
5
VAC 1
PS5 (GRN)
VAC 2 (BRN)
SENSOR
PRESS 1 (ORN)
ACC 5 2 WAY PINCH VALVE NORMALLY OPEN
VAC 2 WASTE (BLK)
(VAR.)
2 WAY PINCH VALVE NORMALLY CLOSED
DRAIN VAC 2 (BRN)
AIR FLOW RESTRICTOR
32060
CHECK VALVE. ARROW SHOWS
DIRECTION OF FLOW
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Figure 2C-2 Sample Loader Flow Diagram
Figure 2C-2 Sample Loader Flow Diagram

12 psi 12" Hg.
5um 3-Way
Filter Valve
Rack Advance
NC Out .010" Orifice
NO
V1
3-Way
Valve
Load Side Cross Transfer
NC Out
.016" Orifice
NO
V2
3-Way
Valve
Unload Side Cross Transfer
NC Out
.016" Orifice
NO
V5
3-Way
Valve
To Mixer Head Up/Down
Air Out
NC
.016" Orifice
NO
V3
3-Way
Valve
5 psi
NC Out
Mixer Bladders
NO
V4
Regulator
32061
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Figure 2C-3 Vacuum/Pressure Flow Diagram
Figure 2C-3 Vacuum/Pressure Flow Diagram
REAGENT & WASTE PANEL
TO AIR
MUFFLER WASTE 62 63 61 64
PRES SENSORS
PUMP
TO AIR
TO AIR
FILTER 1
DILUENT SHEATH
RESERVOIR RESERVOIR
PS1
SENSOR
ACC 1
PRES 1
(12 psi)
2 WAY PINCH VALVE NORMALLY OPEN 12 psi
9 psi
2
5 psi
PS2 AIR FLOW RESTRICTOR 12" Hg
SENSOR VAC 2 (Varies)

ACC 2 CHECK VALVE. ARROW SHOWS
PRES 2 DIRECTION OF FLOW
(9 psi)
3
TRAP
PS3
SENSOR
ACC 3
PRES 3 TRAP TRAP
(5 psi) 67
TO SAMPLE LOADER 17
TO AIR
(SL MODEL ONLY)
MUFFLER
VAC
PUMP
VAC 1 (GRN) WC-1
WC-2
PS4 TO AIR WC-4 WC-3

SENSOR
ACC 4 18
VAC 1 4
(12" Hg)
31 32 97 98
DRAIN
5
VAC 1
PS5 (GRN)
VAC 2 (BRN)
SENSOR
PRESS 1 (ORN)
ACC 5
VAC 2 32062
(VAR.)
DRAIN VAC 2 (BRN)
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Figure 2C-4 Reagent Flow Diagram
Figure 2C-4 Reagent Flow Diagram
REAGENT & WASTE PANEL BLOOD/OUT, FARSIDE

WBC/OUT
HGB LYSE WBC DIL/IN, FARSIDE
HGB/OUT
DILUENT
WBC LYSE BLOOD/IN
EMPTY HGB DIL/IN RBC/OUT RBC DIL/IN, FARSIDE

SENSORS
WBC LYSE
DILUENT SHEATH HGB LYSE

RESERVOIR RESERVOIR
23 28
TOP FILTER (SMALL)

HGB LYSE (BLU)
WBC LYSE (VIO)
91 WBC
66 65
FLOW
DILUENT (RED) CELL
94 96
DILUENT (RED) 12345
WBC LYSE
95 HGB OUT WBC

WBC M. C.
MOTOR-X 20 uL
HGB HGB DIL
12 uL
BLOOD RBC 22
SAMPLE 1.67 uL
VALVE MOTOR-G
RBC RBC DIL
MIXING RBC OUT
CHAMBER HGB 500 uL
FLOW SAMPLE
11 CELL INJECTION
SY1
DILUENT/SHEATH MOTOR-A
SHEATH
HGB LYSE
PRESSURE
WBC LYSE SWITCH
OPEN 27 26
SAMPLE 25 24
PROBE
15 33
2 WAY PINCH VALVE NORMALLY OPEN
MOTOR-E 10 mL 2.5 mL 2.5 mL
2 WAY PINCH VALVE NORMALLY CLOSED 34 RBC WBC HGB
DIL LYSE LYSE 32063
SYRINGE SYRINGE SY2
AIR FLOW RESTRICTOR SY4 SY3
MOTOR-D MOTOR-C MOTOR-B

DIRECTION OF FLOW
CLOSED
MODE
PROBE
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Figure 2C-5 Sample Aspiration Flow
Figure 2C-5 Sample Aspiration Flow Diagram

SAMPLE
SENSOR 2
WBC 2 WAY PINCH VALVE NORMALLY CLOSED
MOTOR-X 20 uL
HGB
BLOOD 12 uL
RBC AIR FLOW RESTRICTOR
SAMPLE 1.67 uL
VALVE
DIRECTION OF FLOW TRAP
ULTRASONIC
SENSOR
12
Y-VALVE
47,48 TO AIR
17
OPEN
SAMPLE
PROBE
38
MOTOR-E WC-2
VENT
TRAP
CLOSED
MODE
32064
PS5
VAC 2 (BRN)
SENSOR OPEN MODE SAMPLE
ACC 5 CLOSED MODE SAMPLE
VAC 2
OPEN/CLOSED MODE SAMPLE
(VAR.)
VAC 2 (BRN) VACUUM #2
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Figure 2C-6 Dilution and Mixing Flow
Figure 2C-6 Dilution and Mixing Flow Diagram
(1) RBC/PLT WBC

TO AIR FLOW
CELL
FILTER
(LARGE) 1 2345
54
53
(2) WBC 52
51 WBC (3) NOC
M. C. PERIS-
55
TALTIC
VENT
PUMP
ACC
MOTOR-G
RBC
MIXING
CHAMBER HGB
FLOW
CELL
41
32066
RBC/PLT STAGING
WBC STAGING
2 WAY PINCH VALVE NORMALLY CLOSED NOC STAGING
STAGING PUMP LINES

AIR FLOW RESTRICTOR WC-1
MIXING CHAMBER VENTS
DIRECTION OF FLOW
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Figure 2C-7 Sample Staging Flow Diagram
Figure 2C-7 Sample Staging Flow Diagram
(1) RBC/PLT WBC

TO AIR FLOW
CELL
FILTER
(LARGE) 1 2345
54
53
(2) WBC 52
51 WBC (3) NOC
M. C. PERIS-
55
TALTIC
VENT
PUMP
ACC
MOTOR-G
RBC
MIXING
CHAMBER HGB
FLOW
CELL
41
32066
RBC/PLT STAGING
WBC STAGING
2 WAY PINCH VALVE NORMALLY CLOSED NOC STAGING
STAGING PUMP LINES

AIR FLOW RESTRICTOR WC-1
MIXING CHAMBER VENTS
DIRECTION OF FLOW
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Figure 2C-8 Sample Delivery Flow Diagram
Figure 2C-8 Sample Delivery Flow Diagram
SHEATH
RESERVOIR
TOP FILTER (SMALL)
91 WBC
65
TO AIR FLOW
DILUENT (RED) CELL
FILTER
(LARGE) 12345
56
(1) RBC
(2) WBC
(3) NOC
VENT
ACC
500 uL
SAMPLE
INJECTION
SY1
MOTOR-A
68
2 WAY PINCH VALVE NORMALLY OPEN SAMPLE DELIVERY

FLOW CELL OUT
2 WAY PINCH VALVE NORMALLY CLOSED WASTE CHAMBER #1 VENT
AIR FLOW RESTRICTOR SHEATH FLOW

WC-1
32067
DIRECTION OF FLOW
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Figure 2C-9 Waste Flow Diagram
Figure 2C-9 Waste Flow Diagram

BLOOD/OUT, FARSIDE
WBC/OUT
HGB/OUT WBC DIL/IN, FARSIDE
BLOOD/IN
WASTE RBC DIL/IN, FARSIDE
HGB DIL/IN RBC/OUT
WBC
FLOW
CELL
12345
56
57
52
WBC
WBC M. C. PERIS-
20 uL HGB
TALTIC
12 uL PUMP
RBC
1.67 uL
MOTOR-G
RBC
MIXING TRAP
CHAMBER HGB
FLOW
12 CELL
13 92
32068
TRAP TRAP
Y-VALVE 93
47,48
21
OPEN
SAMPLE
PROBE
14
WC-2 WC-1
MOTOR-E
WC-4 VENT
WC-3 TRAP
ACC 4 37
VAC 1 36
(12" Hg) CLOSED
MODE
DRAIN PROBE
ACC 5 2 WAY PINCH VALVE NORMALLY OPEN WASTE CHAMBER #1 WASTE

VAC 2 WASTE (BLK) WASTE CHAMBER #2 WASTE
(VAR.)
WASTE CHAMBER #3 WASTE
DRAIN
WASTE CHAMBER #4 WASTE
AIR FLOW RESTRICTOR
MAIN WASTE OUT
DIRECTION OF FLOW
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Figure 2C-10 Vent Flow Diagram
Figure 2C-10 Vent Flow Diagram
TO AIR 2 WAY PINCH VALVE NORMALLY OPEN
MUFFLER
PRES
PUMP
TO AIR AIR FLOW RESTRICTOR
TO AIR
FILTER 1 CHECK VALVE. ARROW SHOWS
TO AIR DIRECTION OF FLOW
FILTER VENT TO AIR

(LARGE)
ACC 1
PRES 1 53
(12 psi)
51 WBC
M. C.
VENT
ACC
RBC
MIXING
CHAMBER HGB
FLOW
CELL
TO AIR
MUFFLER
68
VAC
TO AIR
PUMP
TO AIR 32069
38
WC-1
ACC 4
VAC 1 4
(12" Hg) VENT
TRAP
$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Service Commands
Service Commands DIAGNOSTICS Pressing F12/F1 accesses the following

Commands.
3200 Hidden Screens
SET POINT ENTRY
From the Menu listed, press the function keys indicated to
access the desired Service Screen. LATEX/BLOOD COMPARE
TOGGLE CS/SL
Example: To access the Set Point Entry Screen, enter the
DIAGNOSTICS Menu then press F12/F1. DIAG FUNCTIONS
ERROR LOG
From Main Menu: F12/F8 to go to C:\CD3200 prompt
STORE RAW ON/OFF
DEBUG ON/OFF
Pressing DIAG FUNCTIONS accesses
From C:\CD3200 Type rl to reboot to Initialized the following commands.
prompt: Type rl /nopri to reboot to Ready
PEEK COMMAND
(Use only if Analyzer was in the Ready POKE COMMAND
Mode before entering DOS.)
MOTOR CONTROL
CALIBRATION F12/F1 accesses Dilution Factors
6#0#3DUWV#/LVWV #Table of Contents
6#0#3DUWV#/LVWV
Table of Contents
How to Use This Chapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Sample Loader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

Reagents and Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Tubing Designator/Catalog Number Table . . . . . . . . . . . . 66
Alphabetical Parts List Cross Reference . . . . . . . . . . . . . . . . . 4
PL-1 Power Supply Module . . . . . . . . . . . . . . . . . . . . . . 11 How to Use This Chapter
PL-2 Power Distribution Module & Laser Supply. . . . . . 13
PL-3 Reagent Lines & Fans . . . . . . . . . . . . . . . . . . . . . 16 This chapter contains Parts Identification information for the
PL-4 Flow Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 CELL-DYN 3200 System. It includes:
PL-5 Vacuum/Pressure . . . . . . . . . . . . . . . . . . . . . . . . . 22
PL-6 Aspiration Tower & Open Probe Assemblies). . . . 24 Alphabetical Listing of Parts and corresponding PL
PL-7 Syringe Drivers (AB, CD) . . . . . . . . . . . . . . . . . . . 27 numbers;
PL-8 Peri-Pump, Sample Handler Module #1, Illustrated Parts Lists, showing part location, catalog
& Open Probe Assembly. . . . . . . . . . . . . . . . . . . . 29 number and description.
PL-9 Shear Valve Driver, Motor Driver Module, System Tubing Maps with table of tubing designators,
& Sample Sensor Boards . . . . . . . . . . . . . . . . . . . 31 catalog numbers and descriptions.
PL-10 Laser Bench . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
PL-11 Motor Processor Module. . . . . . . . . . . . . . . . . . . . 36 Table of frequently used supplies and tools.
PL-12 Main Amplifier & Signal Processor Modules . . . . . 38
PL-13 Central Processing Unit/Device Control Module. . 40
PL-14 Flow Control Module, View #1 . . . . . . . . . . . . . . . 42
PL-15 Solenoid Driver Modules 1, 2, & Solenoid
Driver Module/Cable Distribution Module . . . . . . . 45
PL-16 Solenoid Driver Modules 5,6,9 & Cable
Distribution Module #2 . . . . . . . . . . . . . . . . . . . . . 48
PL-17 Main Computer Boards & Computer Assemblies . 51
PL-18 Sample Loader . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
PL-19 Covers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Flow Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Pneumatic Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
6#0#3DUWV#/LVWV Table of Contents
To find information about a part:

From the Alphabetical Listing:
Click on the red PL number to jump to the appropriate
Parts Description/Catalog Number table.
From an illustrated Parts List:
Find the part in the drawing, note its item number, and
click anywhere on the drawing to jump to the
appropriate Parts Description table.
From a Tubing Map:
Find the Tubing Designator on the tubing, e.g., small
silicon tubing is designator S1. Click anywhere on the
Tubing Map to jump to the Tubing Designator/Catalog
Number table.
3 - Parts Lists Reagents and Supplies
Reagents and Supplies
Item Part Number Description

1 99120-01 Calibrator, CELL-DYN 3000
2 93111-01 Ctrl, CELL-DYN 3000 (2.5 mL)
3 99129-01 Ctrl, CELL-DYN 3000 (3.0 mL)
4 03H78-01 Reagent, WBC Lyse (960 mL)
5 03H79-01 Reagent, DIL/STH (20 L)
6 03H80-01 Reagent, HGB/NOC (960 mL)
7 99644-01 Enzymatic Cleaner
8 1360-02 ISE Conditioning Solutions
9 8160580701 Versalube
10 8160600301 5.0 Standard Reference Particles
13 06H63-01 Rack, Sample Loader (1 ea)
14 04H87-01 Racks, Sample Loader (5 ea)
15 92532-01 Connector, Ser Loopback
16 06H71-01 Specifications, Interface
17 8938080601 Disks, Installation (1 & 2)
18 8938070001 Disk, Service
19 06H61-01 Disk, Setup
20 06H90-01 Kit, Software Upgrade Dom.
21 06H91-01 Kit, Software Upgrade Int.
22 8945020201 Fixture, Voltage Divider
6#0#3DUWV#/LVWV Alphabetical Parts List Cross Reference
Alphabetical Parts List Cross Reference Description List #

Bd, Sensor, Sample Tube PL-18
Description List #
Bd, SER Link/KBd Intfc PL-17
3.0 Standard Reference Particles Rea/Sup
Bd, Shear Valve Driver, SVDM PL-9
Bd, Signal Processor Module, SPM PL-12
Bd, Solenoid Driver Module, SDM PL-15 PL-16
Accumulator, Vac/Pres (Lg) PL-5 Bd, Status Alert PL-19
Accumulator, Vac/Pres (SM) PL-5 Bd, Ultrasonic Sensor PL-9
Barcode Reader PL-18 Bd, Video, VGA W/1MB RAM PL-17
Bd, Cable Dist Module, CDM PL-16 Bd, VPM (Vac/Pres Module) PL-5
Bd, Chopper Driver Module, CDB PL-6 PL-7 Bd. Sample Handler Module, SHM PL-8
Bd, CPU/DCM PL-13 Belt, Timing PL-7
Bd, DC Motor Driver Module PL-9 Belt, Timing Spinner Assy PL-6
Bd, Flow Control Module, FCM PL-14 Belt, Timing, GS1 Assy PL-6
Bd, HV/Preamp PL-10 Belt, Timing, GS1 Motor PL-6
Bd, I/O Serial/Parallel PL-17 Bench, Optics (Complete) PL-10
Bd, In-line Sensor PL-3 Block, Wash, Closed Sample PL-6
Bd, Main Amplifier Module PL-12 Block, Wash, Open Sample PL-6
Bd, w/RAM & CPU Mother PL-17 Bottle, Vent Accumulator PL-4
Bd, Motor Processor Module, MPM PL-11 Bottle, Waste (large) PL-4
Bd, PDM (Power Dist. Module) PL-2 Bottle, Waste (small) PL-4
Bd, Photodiode Preamp PL-10 Cable Kit, Internal I/O PL-17
Bd, PRM (Pump Relay Module) PL-5
Cable, 900 PRE-J2 to MAM-J12 PL-10 PL-12
Bd, Sample Handler Module PL-18
Cable, 900 D PRE-J2 to MAM-J14 PL-10 PL-12
Bd, Sample Sensor#2 PL-9
Cable, CDM-J6 to FCM-J2 PL-16
Bd, SDM/CDM PL-15
Description List # Description List #

Cable, CDM2-J10 to SH Sensor PL-16 Cable, FCM-J26 to ILSB-J1 PL-14 PL-3
Cable, CDM2-J4 to VPM-J6 PL-16 Cable, FCM-J4 to SDM9-J10 PL-14
Cable, CDM2-J7 to Ext Waste PL-3 PL-16 Cable, FCM-J5 to PSM(B)-J1 PL-14
Cable, CDM2-J8 to DIL Sensor PL-16 Cable, FCM-J7 to SAB-J1 PL-14 PL-19
Cable, CPU-J1 to SHM1-J16 PL-13 Cable, FCM-J8 to SVDM-J2 PL-14
Cable, CPU-J10 to SPM-J5 PL-12 PL-13 Cable, FCM-J9 to Start SW PL-14
Cable, CPU-J12 to SPM-J7 PL-13 Cable, Floppy Drive INTFC PL-17
Cable, CPU-J17 to SHM2-J16 PL-13 Cable, Hard Drive INTFC PL-17
Cable, CPU-J3 to SPM-J4 PL-13 Cable, HGB Flow Cell to FCM PL-4
Cable, CPU-J6 to MPM-J1 PL-11 PL-13 Cable, HSSL (Internal) PL-13
Cable, CPU-J7 to FCM-J25 PL-13 Cable, HSSL to Monitor PL-17
Cable, CPU-J8 to FCM-J22 PL-13 Cable, Interface, HSSL PL-17
Cable, DCM2-J3 to PSM(B)-J1 PL-16 Cable, MAM-J10 to PD PRE PL-12
Cable, External CPU Bus PL-12 PL-13 Cable, MAM-J12 to PD PRE PL-12
Cable, FCM-J-8 to HGB F. Cell PL-14 Cable, MAM-J15 to SPM-J6 PL-12
Cable, FCM-J1 to CDM1-J6 PL-14 Cable, MAM-J3 to PDM-J4 PL-12
Cable, MUX DAC Ctrl Bus PL-5 Cable, MAM-J4 to CPU-J9 PL-12 PL-13
Cable, FCM-J10 to DIL OP Sen PL-14 Cable, MDM-J1 to SDM4-J8 PL-9
Cable, FCM-J18 to HGB Cell PL-4 Cable, MDM-J2 to SDM4-J7 PL-9
Cable, FCM-J2 to CDM2-J6 PL-14 Cable, MPM-J3 to CDB A-J1 PL-7 PL-11
Cable, FCM-J21 to PDM-J8 PL-14 Cable, MPM-J4 to CDB B-J1 PL-7 PL-11
Cable, FCM-J22 to CPU-J8 PL-14 Cable, MPM-J5 to CDB C-J1 PL-7 PL-11
Cable, FCM-J23 to VPM-J13 PL-14 Cable, MPM-J6 to CDB D-J1 PL-7 PL-11
Cable, FCM-J24 to VPM-J14 PL-14 Cable, MPM-J7 to CDB E-J1 PL-11
Cable, FCM-J25 to CPU-J7 PL-14 Cable, MPM-J7 to CDM-J1 PL-6

Cable, MPM-J9 to CDB G-J1 PL-8 PL-11 PL-14 PL-15
Cable, SDM Data/+5V Bus
PL-12 PL-13 PL-16
Cable, MUX DAC CTRL Bus
PL-14 Cable, SDM5-J10 to CDM2-J1 PL-16
Cable, Nose Cover Sensor PL-8 PL-19 Cable, SDM5-J10 to CDM2-J2 PL-16
Cable, P/O Ultrasonic Sen PL-14 Cable, SDM9-J10 to FCM-J4 PL-16
Cable, PDM-J10 to Hard Drive PL-2 PL-17 PL-5 PL-13
Cable, Serial Periph Bus
Cable, PDM-J11 to Floppy Drive PL-2 PL-17 PL-14
Cable, PDM-J12 to SHM1-J12 PL-2 Cable, SHM1-J1 to GS2 Solenoid PL-8
Cable, PDM-J13 to SHM2-J12 PL-2 Cable, SHM1-J12 to PDM-J12 PL-8
Cable, PDM-J2/J3 to Mother Bd. PL-2 PL-17 Cable, SHM1-J13 to GS1 Motor PL-8
Cable, PDM-J4 to MAM-J3 PL-2 PL-13 Cable, SHM1-J16 to CPU-J1 PL-8
Cable, PDM-J5 to MDM-J3 PL-2 PL-9 Cable, SHM1-J8 to Spin Motor PL-8
Cable, PDM-J6 to MPM-J2 PL-2 PL-11 Cable, SHM1-J9 to Sensors PL-8
Cable, PDM-J8 to FCM-J21 PL-2 PL-5 Cable, SHM2-J10 to MIX Sens PL-18
Cable, PMT Socket PL-10 Cable, SHM2-J11 to Tube Sens PL-18
Cable, Printer, Parallel PL-17 Cable, SHM2-J12 to PDM-J13 PL-18
Cable, PSM (B)-J1 to CDM2-J3 PL-1 Cable, SHM2-J13 to MIX MTR PL-18
Cable, PSM (B)-J3 to SVD-J3 PL-1 Cable, SHM2-J15 to BCD RDR PL-18
Cable, PSM (B)-J4 to PDM-J9 PL-1 PL-2 Cable, SHM2-J16 to CPU-J17 PL-18
Cable, PSM (Back)-J2 to CDM1-J3 PL-1 PL-15 Cable, SHM2-J9 to RACK Sens PL-18
Cable, PSM (T)-J13 to PWR SW PL-1 Cable, SPM-J4 to CPU-J3 PL-12
Cable, PSM (T)-J2 to PRM-J4 PL-1 Cable, SS2-J1 to FCM-J14 PL-9 PL-14
Cable, PSM (T)-J501 to PDM-J1 PL-1 PL-2 Cable, SVDM J3 to PSM (B)-J3 PL-9
Cable, PSM (T)-J7 to XXXFAN PL-1 Cable, SVDM J4 to DC Motor PL-9
Cable, PSM (T)-J8 to XXXFAN PL-1 Cable, SVDM-J1 to Sensors PL-9

Cable, SVDM-J2 to FCM-J8 PL-9 Cover, Right Side PL-19
Cable, Vac1 Wet Sensor PL-5 Cover, SL Bottom PL-19
Cable, Vac2 Wet Sensor PL-5 Cover, SL Left Side Front PL-19
Cable, VPM-J13 to FCM-J23 PL-5 Cover, SL Right Side Front PL-19
Cable, VPM-J14 to FCM-J24 PL-5 Cover, SL Top Skirt PL-19
Cable, VPM-J18 to PRM-J1 PL-5 Cover, Top PL-19
Cable, VPM-J6 to CDM2-J4 PL-5 Cylinder, Air, Mixer Lift PL-18
Cable, Waste Full Sensor PL-3 Cylinder, Air, X & Y Index PL-18
Cable, Y-Valve Sensor PL-8 PL-9 Disk Drive, Floppy, 1.44MB PL-17
Calibrator, CELL-DYN 3000 Rea/Sup Disk Drive, Hard, 540MB PL-17
Cap Assy, Diluent/Sheath PL-3 Disk, Service Rea/Sup
Cap Assy, HGB/NOC Lyse PL-3 Disk, Setup Rea/Sup
Cap Assy, WBC, Lyse PL-3 Disks, Installation (1 & 2) Rea/Sup
Cbl, SDM/CDM-J1 to SDM1-J10 PL-15 Driver Assy, Y-Valve, Complete PL-8
Cbl, SDM/CDM-J2 to SDM2-J10 PL-15 Enzymatic Cleaner Rea/Sup
Chamber, Mixing, RBC/WBC PL-4 Fan Guards & Filter, 3 Pcs PL-3
Clamp, Tubing 1/4 O.D. TM Fan, 115VAC, 4.69 SQ PL-3
Computer Assy, CD3200 PL-17 Filter, 5 Micron, In-line TM
Connector, Ser Loopback Rea/Sup Filter, In-line, Sheath PL-4
Cord, Power 125Vac 10A PL-1 Filter, Pressure Pump PL-5
Cover, Front Nose Section PL-19 Filter, Vent Accumulator PL-4
Cover, Keyboard PL-17 Fitting, Luer Lock PL-7
Cover, Left Front Door PL-19 Fitting, Reagent Inlet PL-3
Cover, Left Side PL-19 Fitting, Waste Outlet PL-3
Cover, Right Front Door PL-19 Fixture, Voltage Divider Rea/Sup

Flow Cell Assy, HGB PL-4 Keyboard, PCAT PL-17
Flow Cell, Optical PL-10 PL-4 PL-5
Kit, Hardware, 3200
Fitting (T4) TM PL-19
Fitting 0.062 X 0.062 X 0.062 TM Kit, Reagent Line (Complete) PL-3
Fitting Barb Conn 0.062" X 0.062" TM Kit, Software Upgrade Dom. Rea/Sup
Fitting Barb RDCR 0.093" X 0.062" TM Kit, Software Upgrade Int. Rea/Sup
Fitting Barb RDCR 0.125" X 0.062" TM Knob, Sample Valve PL-9
Fitting Barb RDCR 0.125" X 0.093" TM Laser, HeNe, 5mw PL-10
Fitting Conn 0.093 X 0.093 TM Mirror Perforated PL-10
Fitting Elb 0.062" X 0.062" X 0.062" TM Mixer Head, Complete PL-18
Fitting Elb 0.093 X 0.093 TM Monitor, 14 VGA Color PL-17
Fitting, L, 1/8 in x 1/8 in TM Motor, Spinner Assy PL-6
Fitting, Reducer, 3/16 in to 1/8 in TM PL-6 PL-7
Motor, Stepper
PL- 8
Fitting RSTR .010 X 1/16 TM
Motor, Stepper, GS1 PL-6
Fitting RSTR .016 X 1/16 TM
Motor, Stepper, Mixer PL-18
Fitting T 0.062" X 0.062" X 0.062" TM
Motor, Y-Valve PL-8
Fitting T 0.062" X 0.125" X 0.062" TM
Needle, Closed Sample PL-6
Fitting T 0.093 X 0.093 X 0.093 TM
Plug TM
Fitting T 0.125" X 0.125" X 0.125" TM
Power Supply Laser PL-2
Fuse, 4A 220/240 (200VAC In) PL-1
Power Supply, AC/DC Main PL-1
Fuse, 5A 220/240 Vac PL-5
Probe Driver Assy, Open PL-6
Fuse, 8A 220/240 (<200VAC In) PL-1
Probe, Open Sample PL-6
Fuse, Fast 1A 250V 3 AG PL-2
Pump Assy, Peristaltic PL-8
Fuse, SB 15A 220/240VAC PL-1
Pump Head, Vac/Pres PL-5
ISE Cleaning Solutions Rea/Sup

Pump Roller Assy PL-8 Switch, Reed, w/Cable PL-18
Pump, Pressure (Complete) PL-5 Switch, Touch Bar PL-19
Pump, Vacuum (Complete) PL-5 Syringe Driver Assy, (AB) PL-7
Rack, Sample Loader (1 ea) Rea/Sup Syringe, Diluent (10 mL) PL-7
Racks, Sample Loader (5 ea) Rea/Sup Syringe, Driver Assy, (CD) PL-7
Reagent, DIL/STH (20 L) Rea/Sup Syringe, Sample Inject, 500 uL PL-7
Reagent, HGB/NOC (960 mL) Rea/Sup Syringe, WBC/HGB (2.5 mL) PL-7
Reagent, WBC Lyse (960 mL) Rea/Sup Tubing Silicon (S2) TM
Regulator, Pressure PL-18 Tubing Silicon (SY) TM
Reservoir, Diluent/Sheath PL-4 Tubing Silicon 1/32 ID x 5/32 OD TM
Sample Loader, Complete PL-18 Tubing Tyg 0.062" ID 0.125" OD Pur TM
Sensor, Overpressure PL-4 Tubing Tyg 0.062" ID 0.125" OD Red TM
PL-6 PL-8 Tubing Tyg 0.062 TM
Sensor, Photodiode & LED
PL-18 PL-19 Tubing Tyg 0.125 ID 0.250 OD Grn TM
Sensor, Sample Detector PL-9 Tubing Tyg 0.125 ID 0.250 OD Yel TM
Sensor, Sample, Ultrasonic PL-8 Tubing Tyg 0.125" ID 0.250" 0D Pur TM
Shear Valve Driver Assy PL-9 Tubing Tyg 0.125" ID 0.250" OD Blk TM
Shield, SHM #1, 2 PL-18 Tubing Tyg 0.125" ID 0.250" OD Grn TM
Smpl Valve Ceramic, Center PL-9 Tubing Tyg 0.125" ID 0.250" OD Or TM
Smpl Valve Ceramic, Front PL-9 Tubing Tyg 0.125" ID 0.250" OD Red TM
Smpl Valve Ceramic, Rear PL-9 Tubing Tyg 0.125 ID 0.250 OD Blu TM
Solenoid, GS2 Stop PL-6 Tubing Tyg 1/32 ID 3/32 OD TM
Speaker Assy PL-17 Tubing Tyg 3/16 ID 5/16 OD Clr TM
Specifications, Interface Rea/Sup Tubing Tyg 3/32 TM
Switch, AC Input, 16A PL-17 Tubing Tyg O.125 ID 0.250 OD Gry TM
6#0#3DUWV#/LVWV #Alphabetical Parts List Cross Reference
Description List #
Tubing Tygon, 1/16 ID TM
Tubing Tygon, 1/8 ID TM
Tower Assy, Aspiration PL-6
Trap Assy, Waste Bottle PL-4
Tube Assy, Waste Outlet PL-3
Tube, Photomult, 900 PL-10
Tube, Photomult, 900 DEP PL-10

Tubing, Peristaltic Pump PL-8
Valve Assy, Solenoid, 3.9KG PL-4
Valve Assy, Solenoid, 3.9KG PL-5
Valve Assy, Solenoid, 5Kg PL-5
Valve Assy, Solenoid, NC PL-4
Valve, Check, Duckbill TM
Valve, Rotary, 3-Port PL-8
Valve, Solenoid, 3-Way PL-18
Versalube Rea/Sup
6#0#3DUWV#/LVWV #PL-1 Power Supply Module (1 of 2)
PL-1 Power Supply Module (1 of 2)
8 9
7 10
11
13/14
5
4
3 1 12
2
6#0#3DUWV#/LVWV #PL-1 Power Supply Module (2 of 2)
PL-1 Power Supply Module (2 of 2)

1 8934000801 Power Supply, AC/DC Main
2 8951078201 Cable, PSM (B)-J4 to PDM-J9
3 8952086901 Cable, PSM (B)-J3 to SVD-J3
4 8952082801 Cable, PSM (Back)-J2 to CDM1-J3
5 8952086601 Cable, PSM (B)-J1 to CDM2-J3
6 8510016601 Fuse, SB 15A 220/240VAC
7 8952087801 Cable, PSM (T)-J2 to PRM-J4
8 8952062001 Cable, PSM (T)-J8 to XXXFAN
9 8952000101 Cable, PSM (T)-J7 to XXXFAN
10 8952000201 Cable, PSM (T)-J13 to PWR SW
11 8952089601 Cable, PSM (T)-J501 to PDM-J1
12 8240051601 Cord, Power 125Vac 10A
13 50065-01 Fuse, 8A 220/240 (<200VAC In)
14 61164-01 Fuse, 4A 220/240 (200VAC In)
6#0#3DUWV#/LVWV #PL-2 Power Distribution Module & Laser Supply (1 of 3)
PL-2 Power Distribution Module & Laser Supply (1 of 3)
5 6
4 7
8
9
10
11
3
2
1 12
13
14

1 8952078201 Cable, PSM (B)-J4 to PDM-J9
2 8952082901 Cable, PDM-J8 to FCM-J21
3 8952054301 Cable, PDM-J6 to MPM-J2
4 8952089601 Cable, PSM (T)-J501 to PDM-J1
5 8952089701 Cable, PDM-J10 to Hard Drive
6 8952089801 Cable, PDM-J11 to Floppy Drive
7 8952061201 Cable, PDM-J3 to Mother Bd.
8 8952086501 Cable, PDM-J4 to MAM-J3
9 8952078901 Cable, PDM-J5 to MDM-J3
10 8952089401 Cable, PDM-J12 to SHM1-J12
11 8952089501 Cable, PDM-J13 to SHM2-J12
12 8960198001 Bd, PDM (Power Dist. Module)
13 8510019201 Fuse, Fast 1A 250V 3 AG
14 8921207601 Power Supply Laser
6#0#3DUWV#/LVWV #PL-3 Reagent Lines & Fans (1 of 3)
PL-3 Reagent Lines & Fans (1 of 3)
4
3
2
6 5
9 10
7 11
8
13
32017 12

1 06H65-01 Kit, Reagent Line (Complete)
2 8921210101 Cap Assy, WBC, Lyse
3 8921205901 Cap Assy, Diluent/Sheath
4 8921211501 Cap Assy, HGB/NOC Lyse
5 92161-02 Tube Assy, Waste Outlet
6 8925089901 Cable, Waste Full Sensor
7 8960091001 Bd, In-line Sensor
8 8952087001 Cable, FCM-J26 to ILSB-J1
9 03484-01 Fan Guards & Filter, 3 Pcs
10 8260402701 Fan, 115VAC, 4.69 SQ
11 8952085701 Cable, CDM2-J7 to Ext Waste
12 8930038301 Fitting, Reagent Inlet
13 8930067601 Fitting, Waste Outlet
6#0#3DUWV#/LVWV #PL-4 Flow Panel (1 of 3)
PL-4 Flow Panel (1 of 3)
3
1
1
2
3
2 2
2 2
7
8 9
6 14
4
13
15 4
10
11
9 12 32019

1 8921122501 Valve Assy, Solenoid, 3.9KG
2 8921016301 Valve Assy, Solenoid, NC
3 8913007601 Kit, Hardware, 3200
4 8921174702 Bottle, Waste (small)
5 8270721901 Filter, Vent Accumulator
6 8921176502 Bottle, Vent Accumulator
7 8921174902 Reservoir, Diluent/Sheath
8 8921206702 Flow Cell Assy, HGB
9 8921150901 Trap Assy, Waste Bottle
10 8510615001 Sensor, Overpressure
11 8921174802 Bottle, Waste (large)
12 8270721901 Filter, In-line, Sheath
13 8921210801 Chamber, Mixing, RBC/WBC
14 8952077801 Cable, HGB Flow Cell to FCM
15 8921217201 Bottle, Waste (small w/tubing)
6#0#3DUWV#/LVWV #PL-5 Vacuum/Pressure (1 of 2)
PL-5 Vacuum/Pressure (1 of 2)
9 10
11
13
12
8 3
22
3 V1
V4
V2
7 3 V3
2 14
V5
4
4 15
6 16
1 1
5 17
2
19
1
21 18
20
6#0#3DUWV#/LVWV #PL 5 Vacuum/Pressure (2 of 2)
PL 5 Vacuum/Pressure (2 of 2)
Item Part # Description
1 8921122001 Valve Assy, Solenoid, 5Kg
2 8913007601 Kit, Hardware, 3200
3 8921173201 Accumulator, Vac/Pres (SM)
4 8921122101 Valve Assy, Solenoid, 3.9KG
5 8921153401 Pump, Vacuum (Complete)
6 8921153001 Pump, Pressure (Complete)
7 8410301801 Pump Head, Vac
8 60160-01 Fuse, 5A 220/240 Vac
9 8960120001 Bd, PRM (Pump Relay Module)
10 8952083401 Cable, VPM-J18 to PRM-J1
11 8921173401 Accumulator, Vac/Pres (Lg)
12 8952087501 Cable, Vac2 Wet Sensor
13 8952052801 Cable, Vac1 Wet Sensor
14 8952082901 Cable, PDM-J8 to FCM-J21/VPM-J12
15 8952083101 Cable, VPM-J13 to FCM-J23
16 8952083201 Cable, VPM-J14 to FCM-J24
17 8952084101 Cable, MUX DAC CTRL Bus
18 8952084901 Cable, Serial Periph Bus
19 8952087901 Cable, VPM-J6 to CDM2-J4
20 8952083401 Cable, VPM-J18 to PRM-J1
21 8960122001 Bd, VPM (Vac/Pres Module)
22 8270040301 Filter, Pressure Pump
6#0#3DUWV#/LVWV #PL-6 Aspiration Tower & Open Probe Assemblies (1 of 3)
PL-6 Aspiration Tower & Open Probe Assemblies (1 of 3)
1
7
2
10
3 5
8
2
6
16
11
12
15
13
14

1 8921208502 Tower Assy, Aspiration
2 8370830101 Sensor, Photodiode & LED
3 8250426601 Belt, Timing, GS1 Assy
4 8250426501 Belt, Timing, GS1 Motor
5 8921209202 Motor, Spinner Assy
6 8250426801 Belt, Timing Spinner Assy
7 03H99-01 Needle, Closed Sample
8 8933040501 Block, Wash, Closed Smple
9 8934005801 Solenoid, GS2 Stop
10 8350602701 Motor, Stepper, GS1
11 8921204601 Probe Driver Assy, Open
12 9960042001 Bd, Chopper Driver Module, CDB
13 8952084201 Cable, MPM-J7 to CDM-J1
14 8921076101 Motor, Stepper
15 8931201401 Block, Wash, Open Sample
16 8930094601 Probe, Open Sample
6#0#3DUWV#/LVWV #PL-7 Syringe Drivers (AB, CD) (1 of 2)
PL-7 Syringe Drivers (AB, CD) (1 of 2)
8
5
5
4
4
7
6
9
4 9
4 2 6 10
9
12
13
3 9
1 6 6
11
5 5
2
6#0#3DUWV#/LVWV #PL-7 Syringe Drivers (AB, CD) (2 of 2)
PL-7 Syringe Drivers (AB, CD) (2 of 2)

1 8921217301 Syringe Driver Assy, (AB)
2 28561-01 Syringe, WBC/HGB (2.5 mL)
3 28560-01 Syringe, Sample Inj (500 uL)
4 04H02-01 Fitting, Luer Lock
5 8250425701 Belt, Timing
7 8952084401 Cable, MPM-J4 to CDB-J1
8 8952015401 Cable, MPM-J6 to CDB D-J1
9 8960042001 Bd, Chopper Driver Module, CDB
10 8952015501 Cable, MPM-J5 to CDB C-J1
11 8921217401 Syringe, Driver Assy, (CD)
12 02H82-01 Syringe, Diluent (10 mL)
13 8952084501 Cable, MPM-J3 to CDB A-J1
6#0#3DUWV#/LVWV #PL-8 Peri-Pump, Sample Handler Module #1, & Open Probe Assembly (1 of 2)
PL-8 Peri-Pump, Sample Handler Module #1, & Open Probe Assembly (1 of 2)
11
12
10 13
9 14
8
7 6
15
1
2
16
16
3
5 4 17 19 18 17
6#0#3DUWV#/LVWV #PL-8 Peri-Pump, Sample Handler Module #1, & Open Probe Assembly (2 of 2)
PL-8 Peri-Pump, Sample Handler Module #1, & Open Probe Assembly (2 of 2)

1 8921137801 Pump Assy, Peristaltic
2 8952056301 Cable, MPM-J9 to CDB G-J1
4 91485-01 Tubing, Peristaltic Pump
5 8921071001 Pump Roller Assy
6 8960191001 Bd. Sample Handler Module, SHM
7 8952089201 Cable, SHM1-J16 to CPU-J1
8 8952088301 Cable, SHM1-J13 to GS1 MOT
9 8952089401 Cable, SHM1-J12 to PDM-J12
10 8952088401 Cable, SHM1-J1 to GS2 SOL
11 8952088201 Cable, SHM1-J8 to Spin Motor
12 8952078001 Cable, Nose Cover Sensor
13 8952087201 Cable, SHM1-J9 to Sensors
14 8921206101 Driver Assy, Y-Valve, Complete
15 8921207701 Motor, Y-Valve
17 8952087101 Cable, Y-Valve Sensor
18 8250900301 Valve, Rotary, 3-Port
19 8370181801 Sensor, Sample, Ultrasonic
6#0#3DUWV#/LVWV #PL-9 Shear Valve Driver, Motor Driver Mod, & Sample Sensor Bds (1 of 3)
PL-9 Shear Valve Driver, Motor Driver Module, & Sample Sensor Boards (1 of 3)
8
9
10
11
12
4
7
5
3
6 2
18
19
P/O Bd.
20
13
17
16
15 14

1 8921204901 Shear Valve Driver Assy
2 8960116701 Sensor, Sample Detector
3 N/A Smpl Valve Ceramics, 3 PCS
4 8932102801 Smpl Valve Ceramic, Rear
5 06H64-01 Smpl Valve Ceramic, Center
6 8932103101 Smpl Valve Ceramic, Front
7 8921167901 Knob, Sample Valve
8 8960222001 Bd, Shear Valve Driver, SVDM
9 8952086201 Cable, SVDM-J2 to FCM-J8
10 8952078501 Cable, SVDM-J1 to Sensors
11 8952086901 Cable, SVDM J3 to PSM (B)-J3
12 8952087401 Cable, SVDM J4 to DC Motor
13 8960194001 Bd, DC Motor Driver Module
14 8952078901 Cable, PDM-J5 to MDM-J3
15 8952079601 Cable, MDM-J2 to SDM4-J7
16 8952079501 Cable, MDM-J1 to SDM4-J8
17 8952087101 Cable, Y-Valve Sensor
18 8370181801 Bd, Ultrasonic Sensor
19 8960116701 Bd, Sample Sensor#2
20 8952082001 Cable, SS2-J1 to FCM-J14
6#0#3DUWV#/LVWV #PL-10 Laser Bench (1 of 2)
PL-10 Laser Bench (1 of 2)
3
2
4
5
1
10
6
8 7 9
5 7
11
6#0#3DUWV#/LVWV #PL-10 Laser Bench (2 of 2)
PL-10 Laser Bench (2 of 2)

1 8921206401 Bench, Optics (Complete)
2 8010131201 Tube, Photomult, 900
3 8921137501 Laser, HeNe, 5mw
4 8570000101 Tube, Photomult, 900 DEP
5 8960100001 Bd, Photodiode Preamp
6 8960101001 Bd, HV/Preamp
7 8921142901 Cable, PMT Socket
8 8952085501 Cable, 900 PRE-J2 to MAM-J12
9 8952085601 Cable, 900D PRE-J2 to MAM-J14
10 8921145201 Flow Cell, Optical
11 8921181501 Mirror Perforated
6#0#3DUWV#/LVWV #PL-11 Motor Processor Module (1 of 2)
PL-11 Motor Processor Module (1 of 2)
2
1
3
4
6#0#3DUWV#/LVWV #PL-11 Motor Processor Module (2 of 2))
PL-11 Motor Processor Module (2 of 2))

1 8960092001 Bd, Motor Processor, MPM
2 8952084701 Cable, CPU-J6 to MPM-J1
3 8952054301 Cable, PDM-J6 to MPM-J2
4 8952084501 Cable, MPM-J3 to CDB A-J1
5 8952084401 Cable, MPM-J4 to CDB B-J1
6 8952015501 Cable, MPM-J5 to CDB C-J1
7 8952015401 Cable, MPM-J6 to CDB D-J1
8 8952084201 Cable, MPM-J7 to CDB E-J1
9 8952056301 Cable, MPM-J9 to CDB G-J1
6#0#3DUWV#/LVWV #PL-12 Main Amplifier & Signal Processor Modules (1 of 2)
PL-12 Main Amplifier & Signal Processor Modules (1 of 2)

1 2
3
13
12
11
10
6
9
8
7
6 5 4
6#0#3DUWV#/LVWV #PL-12 Main Amplifier & Signal Processor Modules (2 of 2)
PL-12 Main Amplifier & Signal Processor Modules (2 of 2)

1 8960195001 Bd, Main Amplifier, MAM
2 8952085501 Cable, 900 PRE-J2 to MAM-J12
3 8952085601 Cable, 900 D PRE-J2 to MAM-J14
4 8960183001 Bd, Signal Processor, SPM
5 8952086501 Cable, MAM-J3 to PDM-J4
6 8952084801 Cable, MAM-J15 to SPM-J6
7 8952050001 Cable, SPM-J4 to CPU-J3
9 8952050501 Cable, CPU-J10 to SPM-J5
10 8952084601 Cable, External CPU Bus
11 8952085401 Cable, MAM-J12 to PD PRE
12 8952085301 Cable, MAM-J10 to PD PRE
13 8952050601 Cable, MAM-J4 to CPU-J9
6#0#3DUWV#/LVWV #PL-13 Central Processing Unit/Device Control Module (1 of 2)
PL-13 Central Processing Unit/Device Control Module (1 of 2)
6
4
3
5
9
11 7
12
10
13
15 14
6#0#3DUWV#/LVWV #PL-13 Central Processing Unit/Device Control Module (2 of 2)
PL-13 Central Processing Unit/Device Control Module (2 of 2)

1 8960182001 Bd, CPU/DCM
3 8952086401 Cable, HSSL (Internal)
4 8952084701 Cable, CPU-J6 to MPM-J1
5 8952084601 Cable, External CPU Bus
6 8952089301 Cable, CPU-J17 to SHM2-J16
7 8952089201 Cable, CPU-J1 to SHM1-J16
10 8952086501 Cable, PDM-J4 to MAM-J3
12 8952050601 Cable, MAM-J4 to CPU-J9
13 8952085201 Cable, CPU-J7 to FCM-J25
15 8952085101 Cable, CPU-J8 to FCM-J22
6#0#3DUWV#/LVWV #PL-14 Flow Control Module, View #1 (1 of 3)
PL-14 Flow Control Module, View #1 (1 of 3)
1
10
8
7
6
5 2
4
21
20
19
18 16
17 15
14
13
12 11

1 8960132001 Bd, Flow Control Module, FCM
4 8952082501 Cable, FCM-J1 to CDM1-J6
5 8952082601 Cable, FCM-J2 to CDM2-J6
6 8952087001 Cable, FCM-J26 to ILSB-J1
7 8952083701 Cable, SDM Data/+5V Bus
8 8952086101 Cable, FCM-J4 to SDM9-J10
9 8952086601 Cable, FCM-J5 to PSM(B)-J1
10 8952083801 Cable, FCM-J7 to SAB-J1
11 8952082901 Cable, FCM-J21 to PDM-J8
12 8952085101 Cable, FCM-J22 to CPU-J8
13 8952083101 Cable, FCM-J23 to VPM-J13
14 8952083201 Cable, FCM-J24 to VPM-J14
15 8952085201 Cable, FCM-J25 to CPU-J7
16 8952038501 Cable, FCM-J-8 to HGB F. Cell
17 8952082001 Cable, SS2-J1 to FCM-J14
18 N/A Cable, P/O Ultrasonic Sen
19 8952083901 Cable, FCM-J10 to DIL OP Sen
20 8952078301 Cable, FCM-J9 to Start SW
21 8952086201 Cable, FCM-J8 to SVDM-J2
6#0#3DUWV#/LVWV #PL-15 Solenoid Dr Mod 1, 2, & Solenoid Dr Mod/Cable Distrib Module (1 of 3)
PL-15 Solenoid Driver Modules 1, 2, & Solenoid Driver Module/Cable

Distribution Module (1 of 3)
MDM-J1
1 2
MDM-J2
V41 6
V38
V32
V36
V34
V33 4
V32 V31
3
6
6
5
3 6 4

V18 V21
V17 V22
V23
V15
V24
V14 V25
V13
V11 V12 V26
V27 V28


1 8960205001 Bd, SDM/CDM
2 8952082801 Cable, PSM (Back)-J2 to CDM1-J3
3 8952083001 Cbl, SDM/CDM-J1 to SDM1-J10
4 8952085801 Cbl, SDM/CDM-J2 to SDM2-J10
5 8960093001 Bd, Solenoid Driver Module, SDM
6#0#3DUWV#/LVWV #PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (1 of 3)
PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (1 of 3)

11
12
2
1
11
8
9
10 2 6 3
8 7 5 4
V98
V97
V96
V95
V94
V93
V92 V91
V68
V67 V57
V66 V56
V65 V55
V54
V64
V63 V53
V62 V52
V61 V51

1 8960093001 Bd, Solenoid Driver Module, SDM
2 8952085901 Cable, SDM5-J10 to CDM2-J1
3 8960108001 Bd, Cable Dist Module, CDM
4 8952085701 Cable, CDM2-J7 to EXT Waste
5 8952083501 Cable, CDM2-J8 to DIL Sensor
6 8952083601 Cable, CDM2-J10 to SH Sensor
7 8952082601 Cable, CDM-J6 to FCM-J2
8 8952086001 Cable, SDM5-J10 to CDM2-J2
9 8952086601 Cable, DCM2-J3 to PSM(B)-J1
10 8952087901 Cable, CDM2-J4 to VPM-J6
12 8952086101 Cable, SDM9-J10 to FCM-J4
6#0#3DUWV#/LVWV #PL-17 Main Computer Boards & Computer Assemblies (1 of 4)
PL-17 Main Computer Boards & Computer Assemblies (1 of 4)
2
7
6
15
15
14
11
10
16
13 12 6
19
18 20
17
20
22
16
21 21

1 8200570201 Disk Drive, Floppy, 1.44MB
2 8921150601 Speaker Assy
3 8952056201 Cable, Floppy Drive INTFC
4 8952089801 Cable, PDM-J11 to Floppy Drive
5 8200570701 Disk Drive, Hard, 540MB
6 8952064801 Cable, Hard Drive INTFC
7 8952089701 Cable, PDM-J10 to Hard Drive
8 8921205401 Computer Assy, CD3200
9 8921211901 Bd Mother, w/RAM & CPU
10 8952061201 Cable, PDM-J2/J3 to Mother Bd
11 8170001301 Bd, Video, VGA w/1MB RAM
12 8960109001 Bd, SER Link/KBd INTFC
13 8952082401 Cable, HSSL to Monitor
14 8170000701 Bd, I/O Serial/Parallel
15 8600028501 Cable Kit, Internal I/O
16 06H74-01 Keyboard, PCAT
17 06H75-01 Cover, Keyboard
18 04H44-01 Monitor, 14 VGA Color
19 8510751801 Switch, AC Input, 16A
20 8952087017 Cable, Interface, HSSL
21 20005-01 Cable, Printer, Parallel
6#0#3DUWV#/LVWV #PL-18 Sample Loader (1 of 5)
PL-18 Sample Loader (1 of 5)
10
32070
#
9
7
13
11
#
12
16
15
14
2
19
18
11
17 19
32071
7
19
7
11 32072
11
2
3
1
4
8 7
6
V5
32073 V4
V3
V2
V1
6#0#3DUWV#/LVWV PL-18 Sample Loader (5 of 5)

1 8960191001 Bd, Sample Handler Module
2 8952058801 Cable, SHM2-J11 to Tube Sens
3 8952058601 Cable, SHM2-J15 to BCD RDR
4 8952089301 Cable, SHM2-J16 to CPU-J17
5 8952089001 Cable, SHM2-J13 to MIX MTR
6 8952089501 Cable, SHM2-J12 to PDM-J13
7 8952088701 Cable, SHM2-J9 to RACK Sens
8 Part of Sensor Cable, SHM2-J10 to MIX Sens
9 8921211601 Sample Loader, Complete
10 8350602501 Motor, Stepper, Mixer
12 8934001701 Barcode Reader
13 8921208301 Mixer Head, Complete
14 8960211001 Bd, Sensor, Sample Tube
15 2880860801 Cylinder, Air, Mixer Lift
16 8510510101 Switch, Reed, w/Cable
17 8450876601 Valve, Solenoid, 3-Way
18 8310842201 Regulator, Pressure
19 8280861301 Cylinder, Air, X & Y Index
20 8932074001 Shield, SHM #1, 2
6#0#3DUWV#/LVWV #PL-19 Covers (1 of 3)
PL-19 Covers (1 of 3)
6 7 8
4 9
6
2 17
8 13
12
8
1 10
8 32074 11
11
20
19
18
32075

1 8931213001 Cover, SL Bottom
2 8932063701 Cover, SL Top Skirt
3 8932073701 Cover, SL Right Side Front
4 8932073801 Cover, SL Left Side Front
5 8921217001 Cover, Front Nose Section
6 8913007601 Kit, Hardware, 3200
7 8931204801 Cover, Left Side
8 8913007601 Kit, Hardware, 3200
9 8931204601 Cover, Top
10 8931204701 Cover, Right Side
11 8932062201 Cover, Right Front Door
13 8952078001 Cable, Nose Cover Sensor
17 8932062101 Cover, Left Front Door
18 8510810901 Switch, Touch Bar
19 8952083801 Cable, FCM-J7 to SAB-J1
20 8960192001 Bd, Status Alert
6#0#3DUWV#/LVWV #Flow Panel
Flow Panel
6#0#3DUWV#/LVWV #Pneumatic Unit
Pneumatic Unit
6#0#3DUWV#/LVWV #Sample Loader
Sample Loader
6#0#3DUWV#/LVWV #Tubing Designator/Catalog Number Table
Tubing Designator/Catalog Number Table

A1 8310817501 Fitting RSTR .016 X 1/16
A2 8310817401 Fitting RSTR .010 X 1/16
C 8310808401 Fitting Conn 0.093 X 0.093
C1 8310802001 Fitting Barb Conn 0.062" X 0.062"
CLP2 8210050201 Clamp, Tubing 1/4 O.D.
CV 8310810201 Valve, Check, Duckbill
EL 8310820001 Fitting Elb 0.093 X 0.093
F1 8270722201 Filter, 5 Micron, In-line
L1 8310807901 Fitting Elb 0.062" X 0.062" X 0.062"
L7 8310805801 Fitting, L, 1/8 in x 1/8 in
P1 03H85-01 Plug
R1 8310805901 Fitting Barb RDCR 0.093" X 0.062"
R4 8310807401 Fitting, Reducer, 3/16 in to 1/8 in
S1 9130542 Tubing, Silicon 1/32 ID x 5/32 OD
S2 9130543 Tubing Silicon (S2)
SY 9130544 Tubing Silicon (SY)
T1 8310801001 Fitting T 0.062" X 0.062" X 0.062"
T3 8310800901 Fitting T 0.125" X 0.125" X 0.125"
T4 31082-01 Fitting (T4)
T5 8310809501 Fitting T 0.062" X 0.125" X 0.062"
T8 8310800701 Fitting T 0.093 X 0.093 X 0.093
6#0#3DUWV#/LVWV #Tubing Designator/Catalog Number Table

Y1 9130548 Tubing Tygon, 1/16 ID
Y2 9130549 Tubing Tygon, 1/8 ID
Y3 8310654201 Tubing Tygon 3/32
Y4 9130555 Tubing Tygon 1/32 ID 3/32 OD
Y5 8310654001 Tubing Tygon 3/16 ID 5/16 OD Clr
Y622 8310662201 Tubing Tygon 0.062" ID 0.125" OD Red
Y626 8310662601 Tubing Tygon 0.062
Y627 8310662701 Tubing Tygon 0.062" ID 0.125" OD Pur
Y630 8310663001 Tubing Tygon 0.125" ID 0.250" OD Blk
Y631 8310663101 Tubing Tygon 0.125 ID 0.250 OD Grn
Y632 8310663201 Tubing Tygon 0.125" ID 0.250" OD Red
Y633 8310663301 Tubing Tygon 0.125" ID 0.250" OD Or
Y634 8310663401 Tubing Tygon 0.125 ID 0.250 OD Yel
Y635 8310663501 Tubing Tygon 0.125" ID 0.250" OD Grn
Y636 8310663601 Tubing Tygon 0.125 ID 0.250 OD Blu
Y637 8310663701 Tubing Tygon 0.125" ID 0.250" 0D Pur
Y638 8310663801 Tubing Tygon O.125 ID 0.250 OD Gry
Y7 8310653501 Tubing Tygon 3/32
Y FIT 8310810801 Fitting 0.062 X 0.062 X 0.062
7#0#5HPRYDO#)#5HSODFHPHQW
Table of Contents Introduction
Step-by-step subassembly removal procedures for field
Shear Valve & Shear Valve Driver (RR-1). . . . . . . . . . . . . . . . .2
replaceable components are described on the following illustrated
Y-Valve (RR-2) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9 pages. Most subassemblies are replaced in reverse order of
Ultrasonic Sensor (RR-3) . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 disassembly, and reassembly is obvious and straightforward.
Open Probe Drive Assembly (RR-4) . . . . . . . . . . . . . . . . . . . .22
Optics Bench (RR-5) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29
Power Supply (RR-6). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35
Vacuum/Pressure Assembly (RR-7) . . . . . . . . . . . . . . . . . . . .39
Tower Assembly (RR-8) . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43
HGB Flow Cell (RR-9) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52
Sample Loader (RR-10) . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57
Syringe Driver Assembly (RR-11) . . . . . . . . . . . . . . . . . . . . . .69
CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 -- August 1998 4-1
7#5HPRYDO#)#5HSODFHPHQW# Shear Valve & Shear Valve Driver (RR-1)
Shear Valve & Shear Valve Figure 4-1: Front Access Doors
Driver (RR-1)
Removal Procedure
1. Ensure that the shear valve is in the
cleaning position using the special
protocols function.
2. With analyzer power off, open the left
and right access doors and remove
the safety cover.
3. Remove the shear valve retaining Figure 4-2: Shear Valve Retaining Screw
screw from the center of the shear
valve.
4. Carefully remove the three ceramic Figure 4-3: Shear Valve Ceramics.
pieces from the shear valve. Set the
fragile center piece aside. Do not
disconnect tubing from the other two
pieces.
5. Remove the top cover (4 screws) and Figure 4-4: Removing Top Cover and Optics Cover
the optics cover (two 6-32 screws and
two 8-32 screws.)
6. Remove the 3 screws that hold the Figure 4-5: Shear Valve Driver MountingScrews
assembly in place.
7. Move the preamplifier cables out of Figure 4-6: Shear Valve Cables J2 and J3.
the way and disconnect cables at J2
and J3 from the Driver Bd.
8. Angle the assembly and remove from Figure 4-7: Shear Valve & Shear Valve Driver Removed.
the back
Replacement Procedure
Replace by following the steps above in
reverse order.
Verification Procedure
See Verification Matrix, Section 5.2.
7#5HPRYDO#)#5HSODFHPHQW# Y-Valve (RR-2)
Y-Valve (RR-2) Figure 4-8: Front Access Doors
Removal Procedure
the front cover.
2. Locate the Y-valve assembly and cut Figure 4-9: Y-Valve Tie Wrap
the tie wrap on the lower left-hand
corner.
3. Disconnect the tubing from the three Figure 4-10: Y-Valve Block Tubing
ports on the valve block
4. Remove the two screws at the top Figure 4-11: Y-Valve Mounting Screws
near the motor and cut the tie wrap to
the left of the wash block.
5. Disconnect the two sensor Figure 4-12: Y-Valve Sensor Connections.

connections from the base of the
assembly.
NOTE: For replacement purposes,
note which socket each comes from
as they are not identified.
6. Remove the top cover (4 screws). Figure 4-13: Top Cover Removal
7. Cut the two tie wraps from around the Figure 4-14: Y-Valve Cable J6
cables above solenoids and the tie
wrap beneath solenoid 13. Disconnect
cable J6 from the Motor Driver Bd.
(located to the left of solenoid 11 in
photo.)
Feed cable through front panel and
remove assembly.
Follow the above steps in reverse order.
7#5HPRYDO#)#5HSODFHPHQW# Ultrasonic Sensor (RR-3)
Ultrasonic Sensor (RR-3) Figure 4-15: Front Access Doors
Removal Procedure

the front cover.
2. Remove the top cover (4 screws.) Figure 4-16: Top Cover Removal
3. Locate the ultrasonic sensor and cut Figure 4-17: Y-Valve Tie Wrap
the tie wrap on the Y-valve
4. Remove the two fluid connections Figure 4-18: Ultrasonic Sensor Fluid Connections
from the left and right of the ultrasonic
sensor.
5. Remove the two screws on the front Figure 4-19: Ultrasonic Sensor Holding Screws
panel which hold the Sensor Bd.
bracket.
6. Disconnect the gray wires from J1 on Figure 4-20: Ultrasonic Sensor Board Connectors
the Sensor Bd and feed connector
through front panel.
Follow the above steps in reverse order.
7#5HPRYDO#)#5HSODFHPHQW# Open Probe Drive Assembly (RR-4)
Open Probe Drive Assembly Figure 4-21: Front Access Doors

(RR-4)
Removal Procedure
Warning Potential Biohazard:
The aspiration needle/probe is sharp
and potentially contaminated with
infectious materials. Avoid contact
with the tips of the needle/probe.

the front cover.
2. Locate and remove the two screws Figure 4-22: Wash Block Holding Screws
holding the wash block to the open
probe assembly and let the wash
block dangle.
3. Remove the 4 screws holding the Figure 4-23: Open Probe Assembly Mounting Screws
assembly to the front panel.
4. Remove the top cover (4 screws). Figure 4-24: Top Cover Removal
5. Remove the right side cover Figure 4-25: Right Side Cover Removal
(4 screws).
6. Loosen the upper and lower Figure 4-26: CPU Door Access
thumbscrews and open the CPU door
assembly to gain access to the back of
the aspiration assembly.
7. Carefully pull aside the white solenoid Figure 4-27: Probe Assembly Removal
wires to remove the drive assembly,
twisting it to the right to remove.
Disconnect the ribbon cable J1 from
the Chopper Driver Bd. and remove
the Probe Assembly.
Replace by following the above steps in
reverse order.
7#5HPRYDO#)#5HSODFHPHQW# Optics Bench (RR-5)
Optics Bench (RR-5) Figure 4-28: Top Cover & Optics Cover Removal
Removal Procedure
'$1*(5=
High Voltage: Assure the
power to the instrument is
off. A high voltage charge
may remain on the laser
connector with the power
off. Do not touch the male
pins. Short male pins to
Chassis.
1. With the analyzer power off, remove

the top cover (4 screws) and the optics
cover (4 screws).
2. Remove the five optics mounting Figure 4-29: Optics Bench Mounting Screws
screws. Remove screws from the
aluminum plate, not the black base of
the Optics Bench.
3. Remove the 4 signal cables from the Figure 4-30: Optics Signal Cables
optics bench. Lay the bundle away
from the optics.
4. Remove the two mixing chambers. Do Figure 4-31: Flow Cell Panel
not disconnect the tubing. Loosen the
two flow cell panel thumbscrews and
open the panel.
5. Disconnect the flow cell tubing (1 - 5) Figure 4-32: Flow Cell Tubing
from the manifold.
6. Being careful not to stress any wires Figure 4-33: Optics Bench Removal
that may be connected to the main
power supply, lift the optics bench out
and place on top of back left corner of
the chassis. Disconnect the brown and
yellow wire from top of the main power
supply and remove optics bench.
reverse order.
7#5HPRYDO#)#5HSODFHPHQW# Power Supply (RR-6)
Power Supply (RR-6) Figure 4-34: Power Supply Connections
Removal Procedure
1. With analyzer power off, remove
optics bench (RR-5).
2. Disconnect the nine connectors
shown on the power supply.
Note 1: Five-pin connections J1, J2,
and J3 are the same.
Note 2: Three-pin connection is
directly above an empty two-pin
connection.
3. Remove power supply mounting Figure 4-35: Power Supply Mounting Screws
screws (6 places).
4. Lift power supply up and out. Figure 4-36: Power Supply Removal
Replacement Procedure Figure 4-37: Power Supply Line Frequency & Line Voltage Select Switches
1. Ensure Line Frequency and Line
Voltage Select switches are set
correctly, according to the photo.
2. Ensure fuse is present and OK.
3. To reinstall, follow the removal steps in
reverse order.
7#5HPRYDO#)#5HSODFHPHQW# Vacuum/Pressure Assembly (RR-7)
Vacuum/Pressure Assembly Figure 4-38: Left Side Panel Removal

(RR-7)
Removal Procedure
NOTE: It is no longer necessary to
remove the Vacuum/Pressure
Assembly when servicing the VPM,
pumps, etc. The Assembly is mounted
on movable slides in 3200 analyzers
built after May, 1998. To access the
Assembly:
1. With the analyzer power off, remove
the left side panel (4 screws).
2. Disconnect the fan power cord and Figure 4-39: Fan Assembly Removal
remove fan assembly (4 screws).
3. Remove the locking screw and pull Figure 4-40: Vacuum/Pressure Assembly Removal
vacuum/pressure assembly out until
unit locks in place.
Replacement Procedure Figure 4-41: Vacuum/Pressure Assembly Return

1. To return assembly to normal position,
push up on clip and push assembly
back in place.
7#5HPRYDO#)#5HSODFHPHQW# Tower Assembly (RR-8)
Tower Assembly (RR-8) Figure 4-42: Front Access Doors
Removal Procedure
Warning Potential Biohazard:

The aspiration needle/probe is sharp
and potentially contaminated with
infectious materials. Avoid contact
with the tips of the needle/probe.
1. With the analyzer power off, open the

left and right access doors and
remove the safety cover.
1. Remove the left and right panels from Figure 4-43: Sample Loader Panels
the rear rail of the sample loader.
2. Remove the screw at the top of the Figure 4-44: Tower Assembly Screw
tower assembly.
3. Loosen the two screws at each end of Figure 4-45: Sample Loader End Screws
the sample loader just one turn.
4. Lift sample loader and slide forward Figure 4-46: Slide Sample Loader Forward
until it stops (about 2).
5. Cut the two tie wraps around cable Figure 4-47: Tower Cable Tie Wraps
bundle in front of flow cell panel.
6. Disconnect J1, J8, J10 and J13 from Figure 4-48: Tower Assembly Cables
the SHM Bd mounted to the flow cell
panel.
7. Perform the following steps: Figure 4-49: Tower Assembly Tubing

a. Disconnect the tubing at aspiration
probe and wash block
b. Remove the glass bubble trap (2
screws). Do not disconnect tubing.
c. Lift the tubing from under the clip.
8. Remove the three hex head (7/64 Figure 4-50: Tower Assembly Removal
allen) screws from the base of the
Tower Assembly and lift the assembly
out.
NOTE: Long screw at base.
reverse order.
7#5HPRYDO#)#5HSODFHPHQW# HGB Flow Cell (RR-9)
HGB Flow Cell (RR-9) Figure 4-51: Front Access Doors
Removal Procedure
1. With analyzer power off, open left and
right access doors and remove front
cover.
2. Remove tubing from the flow cell: two Figure 4-52: HGB Flow Cell Tubing
in front, one on top and one at the
base.
3. Disconnect the inline electrical Figure 4-53: HGB Flow Cell Electrical Connection
connection.
4. Remove the two screws on top of the Figure 4-54: HGB Flow Cell Cover Screws
HGB flow cell to remove the flow cell
cover.
5. Remove the two screws at back of the Figure 4-55: HGB Flow Cell Removal
flow cell to remove it.
reverse order.
7#5HPRYDO#)#5HSODFHPHQW# Sample Loader (RR-10)
Sample Loader (RR-10) Figure 4-56: Front Access Doors
Removal Procedure
Cell-Dyn 3200 Analyzers built after May
1998 do not require sample loader removal
to access front panel.
Follow Part A to access front panel. Follow
Parts A and B to remove and replace the
loader.
Part A
the safety cover.
2. Remove the left and right panels from Figure 4-57: Sample Loader Panels
the rear rail of the sample loader.
tower assembly.
Part B Figure 4-61: Tower Assembly Base Screws

1. Remove the three hex head screws
(7/64 allen) from the base of the
tower assembly.
NOTE: Larger screw at base.
2. Release the clip around the tower Figure 4-62: Tower Assembly Tubing
assembly tubing. Disconnect the two
tubes as shown in the inset. Lay the
assembly on top of the instrument.
3. Disconnect the vacuum and pressure Figure 4-63: Vacuum & Pressure Connections
connections by pressing on the metal
clip.
4. Remove plastic cover from SHM. Figure 4-64: SHM Cables

Disconnect all cables from SHM.
Remove cable clamp (1 screw).
NOTE: Cables are marked for proper
reassembly.
5. Remove cable clamps. Set aside Figure 4-65: Remove Cable Clamps
ribbon cable (J16) and multicolored
cable (J12).
6. Remove loader surround cover by Figure 4-66: Sample Loader Cover Removal
loosening front three screws and
removing left and right end screws (2
at each end).
7. Remove loader mounting screws (two Figure 4-67: Sample Loader Mounting Screws
at each end). Lift loader from brackets.
reverse order.
7#5HPRYDO#)#5HSODFHPHQW# Syringe Driver Assembly (RR-11)
Syringe Driver Assembly Figure 4-68: Front Access Doors

(RR-11)
Removal Procedure
the safety cover.
2. Remove the right panel from the rear Figure 4-69: Sample Loader Right Panel
rail of the sample loader.
tower assembly.
6. Unscrew the luer fittings from top of Figure 4-73: Luer Fittings
each syringe.
7. Remove the four mounting screws for Figure 4-74: Syringe Driver & Spacer Bar
whichever of the two syringe driver
assemblies needs replacing. (Right
side mounting screws shown here.)
Then remove the spacer bar (two
screws) between the two syringe
driver assemblies.
8. Pull out the drive assembly and Figure 4-75: Chopper Driver Ribbon Cables
disconnect the ribbon cables on each
side.
reverse order.
8#0#9HULILFDWLRQ#3URFHGXUHV Table of Contents
8#0#9HULILFDWLRQ#3URFHGXUHV
Table of Contents VP-22 PMT Dynode Voltage Verification/Adjustment . . 22
VP-23 WBC Gain Verification/Adjustment . . . . . . . . . . . 23
VP-24 WBC OPTI-CAL Verification/Adjustment. . . . . . . 24
5.1 How to Use This Chapter . . . . . . . . . . . . . . . . . . . . . 2 VP-25 NOC Gain Adjustment. . . . . . . . . . . . . . . . . . . . . 26
5.2 Verification Matrix . . . . . . . . . . . . . . . . . . . . . . . . . . 3 VP-26 RBC/PLT 0 Gain Verification/Adjustment . . . . . 27
5.3 Verification Procedures . . . . . . . . . . . . . . . . . . . . . . 5 VP-27 RBC/PLT 10 Gain Verification/Adjustment . . . . 28
VP-1 System Voltage Verification/Adjustment. . . . . . . . 5 VP-28 MAM 0 & 10 Log Amplifier Verification . . . . . . . 29
VP-2 A/D Verification/10 Volt Reference VP-29 0 MAM Log Amplifier Verification/Adjustmen. . . 32
Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 VP-30 10 MAM Log Amplifier Verification/
VP-3 MAM Reference Voltage Verification/ Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 VP-31 Aspirate/Vent Needle Verification . . . . . . . . . . . . 34
VP-4 VPM Reference Voltage Verification/ VP-32 GS2 Stop Solenoid Verification . . . . . . . . . . . . . . 35
Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 VP-33 Bar Code Spin Assembly Verification . . . . . . . . . 35
VP-5 HGB Current Verification/Adjustment . . . . . . . . . . 8 VP-34 Tube Height Sensors (S1/S2) Verification. . . . . . 35
VP-6 Motor Power Verification . . . . . . . . . . . . . . . . . . . 8 VP-35 Mixer Up/Down Verification . . . . . . . . . . . . . . . . . 36
VP-7 Motor Operation Verification. . . . . . . . . . . . . . . . . 9 VP-36 Mixer Head Rotation Verification . . . . . . . . . . . . . 36
VP-8 Motor Homing Verification . . . . . . . . . . . . . . . . . . 9 VP-37 Mixer Bladders Verification . . . . . . . . . . . . . . . . . 37
VP-9 Solenoid Bank Verification . . . . . . . . . . . . . . . . . 10 VP-38 Rack Advance & Tube Sensors Verification . . . . 37
VP-10 Individual Solenoid Verification. . . . . . . . . . . . . . 10 VP-39 Cross Transfer Arms & Rack Sensors
VP-11 Bar Code Reader Verification/Alignment . . . . . . 10 Verification. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
VP-12 Specimen Sensor #2 Verification/Adjustment . . 11 VP-40 Rack Bar Code Labels Verification . . . . . . . . . . . 38
VP-13 LSI Loopback Verification. . . . . . . . . . . . . . . . . . 11 VP-41 Mixer Bladders Pressure Verification/
VP-14 MAM & SPM Signal Generator Verification . . . . 12 Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
VP-15 Vacuum & Pressure Retention Verification . . . . 12 VP-42 Shear Valve Driver Lubrication Procedure . . . . . 39
VP-16 Vacuum & Pressure Level VP-43 Optics Bench Cleaning Procedure . . . . . . . . . . . 40
Verification/Adjustment. . . . . . . . . . . . . . . . . . . . 13 VP-44 Optical Flow Cell Cleaning & De-Bubble
VP-17 Vacuum & Pressure Recovery Verification. . . . . 13 Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
VP-18 Laser Replacement/Alignment Procedure . . . . . 14 VP-45 Optical Flow Cell Wetting Procedure. . . . . . . . . . 45
VP-19 Front/Rear Mirror Replacement/Alignment VP-46 Laser Power Output Verification . . . . . . . . . . . . . 46
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 VP-47 Optical Offsets Verification . . . . . . . . . . . . . . . . . 47
VP-20 Optical Flow Cell Verification/Fine Alignment. . . 17 VP-48 Shear Valve Cleaning Procedure . . . . . . . . . . . . 47
VP-21 Optical Flow Cell Coarse Alignment . . . . . . . . . . 21 VP-49 HGB Flow Cell Cleaning Procedure . . . . . . . . . . 48
8#0#9HULILFDWLRQ#3URFHGXUHV 5.1 How to Use This Chapter
VP-50 Open Mode Aspiration Path Cleaning User and System Conventions
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
VP-51 Closed Mode Aspiration Path Cleaning
Menu Selections
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
VP-52 Power-Up CPU/DCM 7-Segment LED Menu selections are identified by square bullets. For example:
Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
1. From Main Menu, press
VP-53 Software Installation Procedure . . . . . . . . . . . . . 51
VP-54 Hard Drive Verification . . . . . . . . . . . . . . . . . . . . 51 DIAGNOSTICS
VP-55 Floppy Drive Verification. . . . . . . . . . . . . . . . . . . 52 MORE
VP-56 Background Counts Verification . . . . . . . . . . . . . 53
TOWER TEST
VP-57 System CV Verification. . . . . . . . . . . . . . . . . . . . 53
VP-58 System Calibration Verification/Adjustment . . . . 54 or:
VP-59 Vacuum Accumulator #1 Rinsing Procedure . . . 54
a. Press
VP-60 Vacuum Accumulator #2 Rinsing Procedure . . . 55
VP-61 Hard Drive Formatting . . . . . . . . . . . . . . . . . . . . 56 ACCEPT
VP-62 DOS Installation . . . . . . . . . . . . . . . . . . . . . . . . . 57 to enter the new factors for the parameters that need
VP-63 CS/SL SETUP . . . . . . . . . . . . . . . . . . . . . . . . . . 57 calibration.
VP-64 Backup Procedure . . . . . . . . . . . . . . . . . . . . . . . 58
VP-65 Restore Setup. . . . . . . . . . . . . . . . . . . . . . . . . . . 58 User Entries
VP-66 List Mode Data Collection. . . . . . . . . . . . . . . . . . 58
VP-67 Download HSSL Com. . . . . . . . . . . . . . . . . . . . . 59 User-typed entries are shown in bold, italicized type. For
example:
5.1 How to Use This Chapter a. At C:\ prompt type CD\RAWFILE, and press
ENTER
This chapter contains:
b. Type DIR, and Press
Verification Matrix containing all VPs required to ensure
ENTER
System integrity after a part is replaced
Step-by-step illustrated procedures to verify, align, calibrate,
and maintain components, assemblies, and functions of the
CD3200 Analyzer and Autoloader
A list of conditions indicating the need to perform a
particular procedure
Required tools and materials for each procedure
5.2 Verification Matrix ASSOCIATED VPs

REPLACED ASSEMBLY
(Perform In Order Listed)
ASSOCIATED VPs VP-35, VP-36, VP-37, VP-38,
REPLACED ASSEMBLY Sample Handler Module #2
(Perform In Order Listed) VP-39, VP-40, VP-41
VP-23, VP-19, VP-26, VP-56, HGB Flow Cell VP-5, VP-56, VP-57, VP-58
0 Degree Preamp
VP-57, VP-58
Shear Valve Driver Module VP-7, VP-57
VP-23, VP-19, VP-27, VP-56,
10 Degree Preamp Motor Driver Module VP-7
VP-57, VP-58
Sample Sensor #2 VP-12
VP-22, VP-23, VP-56, VP-57,
90 Degree PMT Preamp Pump Relay Module VP-17, VP-16
VP-58
VP-22, VP-23, VP-56, VP-57, Barcode Reader VP-10, VP-40
90 Degree PMT Preamp Aspiration Tower VP-31, VP-32, VP-33, VP-34
VP-58
VP-3, VP-28, VP-23, VP-26, VP-35, VP-36, VP-37, VP-38,
Main Amplifier Module Sample Loader
VP-27, VP-56, VP-57, VP-58 VP-39, VP-40, VP-41
VP-23,VP-26, VP-27, VP-56, VP-45, VP-22, VP-20, VP-23,
Signal Processor Module Optics Bench VP-24, VP-25, VP-26, VP-27,
VP-57, VP-58
VP-67, VP-52, VP-2, VP-56, VP-56, VP-57, VP-58
CPU/DCM VP-18,VP-20, VP-23, VP-24,
VP-57, VP-58
Flow Control Module VP-5, VP-9 Laser Head VP-25, VP-26, VP-27, VP-56,
VP-57, VP-58
VP-4, VP-16, VP-56, VP-57,
Vacuum/Pressure Module VP-19, VP-20, VP-23, VP-24,
VP-58
Optics Bench Mirror VP-25, VP-26, VP-27, VP-56,
Motor Processor Module VP-6, VP-7
VP-57, VP-58
Power Supply Module VP-1, VP-56, VP-57, VP-58
VP-19, VP-20, VP-23, VP-24,
Laser Power Supply VP-46 Optical Flow Cell
VP-25, VP-26, VP-27
Solenoid Driver Module VP-6, VP-7, VP-56, VP-57,
VP-9 Syringe Driver Assembly
SDM/CDM VP-58
Sample Handler Module #1 VP-31, VP-32, VP-33, VP-34 VP-6, VP-8, VP-7, VP-56,
Peri-Pump Assembly
VP-57, VP-58
ASSOCIATED VPs
REPLACED ASSEMBLY
(Perform In Order Listed)
S. Valve Center Ceramic VP-1, VP-56, VP-57, VP-58
Hard Disk Drive VP-53, VP-54
Floppy Disk Drive VP-55
8#0#9HULILFDWLRQ#3URFHGXUHV VP-1 System Voltage Verification/Adjustment
5.3 Verification Procedures Table 5-1 Voltage Specifications
Test Point Function Range Ripple

VP-1 System Voltage Verification/Adjustment
PSM-TP4 Analog Ground N/A N/A
PSM-TP2 +12 VDC UREG 15.0V-19.6V < 3.0V PP
Required Tools & Materials:
# 2 Phillips Screwdriver PSM-TP1 +24 VDC UREG 28.9V-37.7V < 7.0V PP
Digital Voltmeter PSM-TP7 N/A N/A N/A

PSM-TP6 N/A N/A N/A
Clip Leads
PSM-TP3 +15 VDC 14.6V-15.4V < 75MV PP
Potentiometer Adjustment Tool
PSM-TP5 -15 VDC 14.6V-15.4V < 75MV PP
Verification Procedure: N/A -12 VDC 11.50-12.50V <75MV PP
1. Open right side door to gain access to power supply. N/A +12 VDC 11.50-12.50V <75MV PP
N/A +5 VDC 5.00V-5.40V <75MV PP
2. Refer to Figure 5-1. Connect voltmeter ground lead to TP4
on power supply module. N/A +24 VDC REG 24.0-25.0 <75MV PP
3. Verify the voltages according to Table 5-1.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-2 A/D Verification/10 Volt Reference Adjustment
Figure 5-1 Power Supply Voltage Testpoints
REAR TO P
15 A S B (+ 1 2V )
F1
ALARM
T P 1 + 24 V
F2 -12V
T P 2 + 12 V
15 A S B (+ 2 4V ) + 12 V
T P 3 + 15 V
PSM + 24 V
TP4 GND
GND
TP5 -15V
+ 5V
10 0V
TP6 RET
+ 10 0V
TP7 3 2 0 76
VP-2 A/D Verification/10 Volt Reference Required Tools & Materials:

Adjustment # 2 Phillips Screwdriver
The CELL-DYN 3200 utilizes an A/D converter to convert all Digital Voltmeter
critical voltages levels to a 12-bit digital code. This code is read Clip Leads
by the computer and these voltages are then displayed on the Potentiometer Adjustment Tool
VOLTAGE READINGS screen in DIAGNOSTICS. The
following procedure verifies the accuracy of this A/D Converter
by using the A/D to read the 9.901 volt reference generated on Verification Procedure:
the CPU/DCM and is also used to set the level of the 10 volt 1. Open right side door to gain access to the CPU/DCM.
reference.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-3 MAM Reference Voltage Verification/Adjustment
2. Locate the CPU/DCM on the right side door and connect Verification Procedure:
the positive lead of the DVM to TP1 and the negative lead
to TPAGND. 1. Connect positive lead of DVM to TP2 and negative to TP7.
3. Adjust R4 for a reading of 10.00 volts +/- 0.01V. 2. If necessary, adjust R86 for a reading of 10.00 volts +/-
0.01V.
4. Move the positive lead to TP3 (9.901V reference) and
verify that the meter reading is 9.90 volts +/- 0.03V.
VP-4 VPM Reference Voltage
5. Move the positive lead to TP2 (99mV reference) and verify Verification/Adjustment
that the meter reading is 0.099 volts +/- 0.003V.
6. From Main Menu, press Required Tools & Materials:
DIAGNOSTICS # 2 Phillips Screwdriver
MORE Digital Voltmeter
VOLTAGE READINGS Clip Leads
7. Press [VOLTAGE READINGS] and observe the 9.901V Potentiometer Adjustment Tool
Reference on the SPM. Verify by repeatedly pressing the
key that the voltage displayed is 9.90 volts +/- 0.05V.
Verification Procedure:
1. Open flow panel door to gain access to the VPM.
VP-3 MAM Reference Voltage
Verification/Adjustment 2. Connect positive lead of DVM to TP2 (VREF+) and
negative to TP1 (VREF-).
Required Tools & Materials: 3. If necessary, adjust R16 for a reading of 10.00 volts +/-
0.01V.
# 2 Phillips Screwdriver
Digital Voltmeter
Clip Leads
Potentiometer Adjustment Tool or Small Slotted
Screwdriver
8#0#9HULILFDWLRQ#3URFHGXUHV VP-5 HGB Current Verification/Adjustment
VP-5 HGB Current Verification/Adjustment 6. Move the cursor to HGB CURRENT; enter new setting,
and press [ENTER]. A change of 50 will yield a voltage
This procedure adjusts the HGB LED current to yield a HGB change of approximately 0.3 volts.
output voltage of 5.00 volts with the HGB Flow Cell filled with
Reference Solution. 7. Press
SET ANALYZER
Required Tools & Materials: RETURN
None RETURN
8. Press
VOLTAGE READINGS
and repeat steps 2 through 8 until the voltage is within
DIAGNOSTICS specifications.
MORE
VOLTAGE READINGS VP-6 Motor Power Verification
2. Move the cursor to HGB output, and press This procedure verifies the winding current of the system
SELECT stepper motors.
FINISH SELECT
3. Run one background count to insure that the HGB mixing
chamber is full of liquid. None
4. Verify that the HGB OUTPUT voltage is 5.00 volts Verification Procedure:
+0.2/-0.00 volts. If the voltage is within range, skip steps 5
through 8. 1. From Main Menu, press
5. Press DIAGNOSTICS
MORE MORE
to the first diagnostic screen, and press MOTOR OPERATION
F12 2. Press
F1 MOTOR PWR CHECKING
and wait for the display.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-7 Motor Operation Verification
3. Verify each motor meets the specifications shown in Table 2. Press

5-2. EXERCISE MOTORS
type the letter of the suspect assembly, and press
Table 5-2 Motor Power Specifications
ENTER
Phase 0 Phase 0 Phase 0 Phase 3
3. Verify that the assembly moves smoothly without stopping
Motor Low Medium High High or jerking.
A 1.5-2.1 3.4-4.2 4.9-5.9 4.9-5.9 4. Use the [SHEAR VALVE] and [Y VALVE] keys to exercise
the shear valve and Y valve.
B .6-1.2 2.5-3.1 4.2-5.0 4.2-5.0
C .6-1.2 2.5-3.1 4.2-5.0 4.2-5.0 VP-8 Motor Homing Verification
D .6-1.2 2.5-3.1 4.2-5.0 4.2-5.0 This procedure allows visual verification of the homing
operation of the system motors by running a homing routine.
E 1.5-2.1 3.4-4.2 4.9-5.9 4.9-5.9
G 1.5-2.1 3.4-4.2 4.9-5.9 4.9-5.9 Required Tools & Materials:
None
VP-7 Motor Operation Verification
This procedure allows visual verification of the operation of the
system motors by running an exercise routine. 1. From Main Menu, press
DIAGNOSTICS
Required Tools & Materials: MORE
None MOTOR OPERATION
2. Press
HOME MOTORS
type the letter of the suspect assembly, and press
DIAGNOSTICS
ENTER
MORE
3. Verify that the assembly moves smoothly without stopping
MOTOR OPERATION
or jerking.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-9 Solenoid Bank Verification
VP-9 Solenoid Bank Verification Verification Procedure:

This procedure allows visual verification of the operation of a 1. From Main Menu, press
bank of eight solenoids by running an exercise routine. DIAGNOSTICS
MORE
SOLENOID OPERATION
None
2. Press
Verification Procedure: STEP SOLENOID
1. From Main Menu, press type the number of the starting solenoid, and press
DIAGNOSTICS ENTER
MORE 3. Use the UP/DOWN ARROW KEYS, and verify that the
solenoids are opened when commanded.
SOLENOID OPERATION
2. Press
VP-11 Bar Code Reader Verification/Alignment
CYCLE BANK
type the number of the bank, and press Required Tools & Materials:
ENTER Specimen Tube With Bar Code Label
3. Verify that the solenoids are opened in ascending order.
VP-10 Individual Solenoid Verification 1. From Main Menu, press
This procedure allows visual verification of the operation of a DIAGNOSTICS
bank of eight solenoids by running an exercise routine. MORE
BAR CODE ALIGNMENT
2. Record the tube bar code number, and place tube at the
None read station.
3. Press
BAR CODE ALIGNMENT
8#0#9HULILFDWLRQ#3URFHGXUHV VP-12 Specimen Sensor #2 Verification/Adjustment
4. Verify that the number displayed on the screen matches NOTE: The voltage at TP4 should be <0.50V when air or
the recorded number, and the read rate is constant. liquid is passing through the sensor, and >3.00V with the
diluted control.
5. If necessary, loosen the locking screws, and align the
position of the reader until the read rate is constant. 7. Run a background count, and verify a reading of (0.50V.
6. Tighten the locking screws and re-verify. 8. While observing the tubing through specimen sensor #2,
place the diluted low control under the open probe, and
manually open valve #12 until the dilution is aspirated
VP-12 Specimen Sensor #2
approximately 2 inches past specimen sensor #2.
Verification/Adjustment
9. Verify a reading of >3.00V.
Required Tools & Materials: 10. Manually open solenoid 13 to remove blood from lines,
and run a background count.
CELL-DYN 3200 Diluent/Sheath
Low Abnormal Control 11. Run another background count, and verify that the LED on
the sensor board does not flicker.
DVM with Clip Leads
12. Disconnect the DVM from the specimen sensor board.
Potentiometer Adjustment Tool
Verification Procedure: VP-13 LSI Loopback Verification

1. Remove top and top right covers.
2. Run controls and insure that results are within Loopback Connector PN 9211532
specifications.
3. Create a specimen dilution using LOW ABNORMAL Verification Procedure:
CONTROL material and CELL-DYN 3200 Diluent/Sheath
that gives an RBC value of 0.8MEG/ul, +/- .03. 1. Turn power off, install loopback connector at COM1 (top
serial connector) on PC module, and power-up and
4. Connect the positive lead of the DVM to TP1 and the initialize instrument.
negative lead to TP3 on the specimen sensor board.
5. Adjust R1 for a reading of 2.00 +/-0.01 volts.
6. Move positive lead to TP4.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-14 MAM & SPM Signal Generator Verification
2. From Main Menu, press VP-15 Vacuum & Pressure Retention

DIAGNOSTICS Verification
MORE This procedure tests the ability of the system to retain the
SERIAL TEST appropriate vacuum and pressure levels. A failure indicates a
leak in the system.
3. Press
TRANSMIT MESSAGE Required Tools & Materials:
and verify that the message received matches the
None
message sent: CELL-DYN serial interface test.
4. Press Verification Procedure:
STOP TRANSMISS 1. Ensure that the instrument is in the READY mode.
RETURN
2. Verify that off-time of the vacuum pump is 2 minutes or
RETURN greater between automatic recovery cycles.
5. Turn power off, and remove loopback connector. 3. Verify that off-time of the pressure pump is 2 minutes or
greater between automatic recovery cycles.
VP-14 MAM & SPM Signal Generator
Verification

None
Not Available At This Time
8#0#9HULILFDWLRQ#3URFHGXUHV VP-16 Vacuum & Pressure Level Verification/Adjustment
VP-16 Vacuum & Pressure Level to the first diagnostic screen and press
Verification/Adjustment F12
F1
Required Tools & Materials: Use the following equation to calculate the new setting(s).
None New Setting = Target Level X Current Setting
Actual Level
6. Move the cursor to the appropriate vacuum(s) or
1. Ensure that the instrument is in the Open Sampler mode. pressure(s), enter new setting(s) and press
2. From Main Menu, press ENTER
DIAGNOSTICS 7. Press
MORE SET ANALYZER
VOLTAGE READINGS RETURN
3. Observe the vacuum and pressure levels and compare RETURN
with Table 5-3.
NOTE: If the level is reduced the vacuum or pressure
Table 5-3 Vacuum & Pressure Specifications must be bled off to the new level before performing step 8.
A count cycle may be used for bleeding.
Vacuum/Pressure Specification
8. Go to VOLTAGE READINGS, and repeat steps 3 through
Pressure #1 12.5-13.5 psi 8 until the level(s) are within specifications.
Pressure #2 8.5-9.5 psi
Pressure #3 4.0-4.5 psi VP-17 Vacuum & Pressure Recovery
Vacuum #1 12.5-13.5 Hg Verification
Vacuum #2 3.1-3.5 Hg This procedure tests the ability of the vacuum and pressure
pumps to return from atmosphere to the appropriate levels. A
4. If all the vacuum and pressure levels are within failure indicates that the pump under test is defective or there is
specifications, do not perform steps 5 through 8. a gross leak in the system.
5. Press
MORE
8#0#9HULILFDWLRQ#3URFHGXUHV VP-18 Laser Replacement/Alignment Procedure
Required Tools & Materials: Verification Procedure:

None 1. Ensure instrument power is off. Remove top cover, both
optics covers, and open flow cell access door.
'$1*(5=#+,*+#92/7$*(#Assure the power
to the instrument is off. A high voltage charge may
DIAGNOSTICS remain on the laser connector with the power off.
MORE Do not touch the male pins. Disconnect the laser
connector and short both male pins to the
to VACUUM TEST and PRESSURE TEST.
instrument chassis.
2. Press
2. Disconnect laser connector and short both male pins to
VACUUM TEST instrument chassis.
and verify that the elapsed time is 4.5 seconds or less.
3. Remove Laser Clamps and replace Laser.
3. Press
4. Replace clamps and tighten until snug but Laser can still
PRESSURE TEST be moved.
and verify that the elapsed time is 3.5 seconds or less. 5. Move laser body until 3/4 inch extends beyond front laser
clamp and rotate laser body until front label is on top.
VP-18 Laser Replacement/Alignment
6. Connect laser connector, ensure laser shutter is closed,
Procedure
turn on power, and prime instrument. Allow a 15-minute
'$1*(5=#/$6(5#/,*+7 Avoid direct laser warm-up period.
exposure to laser beam. Refer to Introduction 7. Refer to Figure 5-4. Mount Power Meter in beam path.
Section 1.7 for detailed safety information. Open Laser Shutter and verify a reading of no less
than 5 mW.
8. If you have a polarizing filter, skip step 9.
9. Scribe optics bench on right and front sides of beam
.050 Allen Wrench
splitter/polarizer assembly, and remove assembly from
Optics Alignment Kit Cat. #8921183801 90o path.
DVM with Clip Leads
Laser Power Meter
8#0#9HULILFDWLRQ#3URFHGXUHV VP-18 Laser Replacement/Alignment Procedure
10. Place polarizing filter or beam splitter/polarizer in beam repeated several times to attain proper alignment. Record
path between laser and power meter with beam splitter power level through slit and, if necessary, replace beam
facing laser. splitter/polarizer in normal position.
11. While observing power reading, rotate the laser body 19. Connect the negative lead of the DVM to TP2 and the
clockwise. As power level decreases, change scales as positive lead to TP1 on the 0o photodiode PCB.
needed.
20. Refer to Figure 5-2. Manually open solenoid 56 and
12. Rotate laser body until power is at minimum on the 20 uW remove tubing from port #1 on the flow cell distribution
scale. manifold.
13. Tighten laser clamps and verify that power level does not Figure 5-2 Flow Cell Plumbing.
change more than +/- 3 uw and remove beam
splitter/polarizer from beam path. Laser is now aligned for 5
56
true vertical polarization.
14. Place alignment post (#9420071) in front tooling hole and
center beam on hole using X and Y alignment screws on
rear mirror. if alignment post is not available, center beam 91 WBC
on cylindrical lens. Placing scotch tape on rear of FLO W
cylindrical lens will aid in centering beam. 3 C EL L
FLO W C E LL M AN IFO LD
15. Remove alignment post and place power meter behind 4
57
forward slit. 54
16. Use X alignment (.050" allen wrench) screw on rear mirror 1 2

52
to obtain maximum power through forward slit. Maximum 32 077 1 2 3 4 5
power through slit should be approximately 1/5 of laser
power. 21. Observe the 90o slit. When two red images of the flow cell
walls appear on the slit, close solenoid 56 and reconnect
17. Use X alignment screw on front mirror to move beam tubing to port #1.
toward alignment hole in center of obscuration bar.
22. Adjust the Y alignment screw on the front mirror for a
18. Repeat steps 16 through 18 until maximum power is being minimum reading on the DVM. This reading should be less
transmitted through slit and beam is centered horizontally than 0.300 volts.
on hole in center of obscuration bar. This may have to be
23. Adjust the Y alignment screw on the rear mirror until the
red images are in the center 1/3 of the 90o slit.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-19 Front/Rear Mirror Replacement/Alignment Procedure
24. Repeat steps 22 and 23 until the red images are in the 2. Remove, replace, and secure defective mirror (front
center 1/3 of the slit and the DVM voltage is at minimum. or rear).
The minimum voltage should be less than 0.200 volts.
3. Place alignment post (#9420071) in front tooling hole and
25. Re-check power transmitted through forward slit. If it has center beam on hole using X and Y alignment screws on
changed, a slight X alignment of the rear mirror is rear mirror.
necessary.
4. Remove alignment post and place power meter behind
26. Replace both optics covers. forward slit.
27. Perform Optical Flow Cell Verification/Fine Alignment 5. Use X alignment (.050" allen wrench) screw on rear mirror
VP-20 and, if necessary, perform Optical Flow Cell Coarse to obtain maximum power through forward slit. Maximum
Alignment VP-21, and repeat VP-20. power through slit should be approximately 1/5 of laser
power.
VP-19 Front/Rear Mirror 6. Use X alignment screw on front mirror to move beam
Replacement/Alignment Procedure toward alignment hole in center of obscuration bar.
7. Repeat steps 4 through 6 until maximum power is being
'$1*(5=#/$6(5#/,*+7 Avoid direct transmitted through slit and beam is centered horizontally
exposure to laser beam. Refer to Introduction on hole in center of obscuration bar. This may have to be
Section 1.7 for detailed safety information. repeated several times to attain proper alignment. Record
power level through slit and, if necessary, replace beam
Required Tools & Materials: splitter/polarizer in normal position.
# 2 Phillips Screwdriver 8. Connect the negative lead of the DVM to TP2 and the
positive lead to TP1 on the 0o photodiode PCB.
.050 Allen Wrench
Optics Alignment Kit Cat. #8921183801 9. Refer to Figure 5-3. Manually open solenoid 56 and
remove tubing from port #1 on the flow cell distribution
DVM with Clip Leads manifold.
Laser Power Meter
1. Remove top cover, both optics covers, and open flow cell
access door.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-20 Optical Flow Cell Verification/Fine Alignment
5
VP-20 Optical Flow Cell Verification/Fine
56 Alignment
'$1*(5=#/$6(5#/,*+7 Avoid direct
WBC exposure to laser beam. Refer to Introduction
91
FL O W Section 1.7 for detailed safety information.
3 CELL
FL O W C E LL M A NIFO L D The alignment of the optical flow cell is one of the most critical
4
54
57 alignments performed on the CELL-DYN 3200. It must be
1 2 optimized so that the instrument can properly perform the WBC
52 5-Part Differential measurements. However, if this alignment is
32077 1 2 3 4 5
not really needed, it is best that it not be performed. Therefore,
Figure 5-3 Flow Cell Plumbing if the instrument is within the prescribed specifications, do not
10. Observe the 90o slit. When two red images of the flow cell attempt to align the optical flow cell.
walls appear on the slit, close solenoid 56 and reconnect
tubing to port #1. Required Tools & Materials:
11. Adjust the Y alignment screw on the front mirror for a # 2 Phillips Screwdriver
minimum reading on the DVM. This reading should be less 7.00 Diameter Beads, PN 1606004
than 0.300 volts.
12. Adjust the Y alignment screw on the rear mirror until the
Clean Container
red images are in the center 1/3 of the 90o slit.
13. Repeat steps 11 and 12 until the red images are in the Verification Procedure:
center 1/3 of the slit and the DVM voltage is at minimum.
The minimum voltage should be less than 0.200 volts. 1. Prepare the WBC Beads solution.
a. Place 2mL of diluent in a clean container.
14. Re-check power transmitted through forward slit. If it has
changed, a slight X alignment of the rear mirror is b. Add 15 drops of 7.00 beads and mix well.
necessary. 2. From Main Menu, press
15. Replace both optics covers. DIAGNOSTICS
16. Perform Optical Flow Cell Verification/Fine Alignment MORE
VP-20 and, if necessary, perform Optical Flow Cell Coarse WBC DATA
Alignment VP-21, and repeat VP-20.
3. Press 7. Loosen the locking screw on the lower (y-axis)

CALC CV micrometer, and cover the opening with black cloth to
block ambient light.
4. Place Beads solution under probe and press TOUCH
PLATE to aspirate Beads and run a count cycle. 8. Place the 7.00 beads solution under the probe, and
press
5. Refer to Table 5-4 and compare the CVs to those
EXTENDED WBC COUNT
displayed on the screen. If the displayed CVs are less than
those from the table, the flow cell does not need START
alignment. to aspirate the Beads and start the cycle.
NOTE: If any or all of the CVs are out-of-specification, the 9. Observe the 0o and 10o display on the left side of the
flow cell must be completely aligned. The 0o and 10o screen. When dots appear on the screen, adjust the lower
channels must be aligned first by adjusting the Y-axis micrometer until the tightest possible grouping of dots and
alignment (lower) micrometer and then the 90o and 90o lowest possible CVs are attained. If the cycle stops before
depolarized must be aligned by adjusting the X-axis this is accomplished, repeat step 8 and try again.
alignment (right) micrometer. 10. Place Beads solution under probe and START SWITCH
Table 5-4 WBC Mean Channel & CV Specification to aspirate Beads and run a count cycle.
Channel Mean Range CV 11. Press
0o 35 32-38 <3.5% CALC CV
10o 65 60-70 <3.5% when the cycle is completed. If the 0o and 10o CVs are
within specifications, continue with the procedure. If not,
90o * Derived From Opti-Cal +/- 5 Channels <12.5%
o
repeat steps 8 through 10.
90 Dep. * Derived From Opti-Cal +/- 5 Channels <12.5%
NOTE: If repeated alignment does not bring the CVs
NOTE: * These values must be obtained from the within specifications, further troubleshooting is required to
Beads auto-gain default table, or by performing the isolate the problem. The first considerations are a
Opti-Cal procedure. contaminated or defective flow cell or a defective laser.
The last consideration should be that of a misaligned
6. Open the flow cell access door, located at the top left of
optical bench, since once properly aligned and secured,
the Analyzer.
none of the assemblies should move under normal
conditions.
12. Loosen the locking screw on the right (x-axis) micrometer,

and cover the opening with black cloth to block ambient
light. Then press the START key to aspirate the Beads
and start the cycle.
13. Observe the 90o and 90o depolarized display on the right
side of the screen. When dots appear on the screen,
adjust the micrometer to attain the greatest deflection to
the right and up. CVs are not as important as in the 0o and
10o channels.
14. Place Beads solution under probe and press START
SWITCH to aspirate Beads and run a count cycle.
15. Press the key when the cycle is completed. If the 90o and
90o depolarized CVs are within specifications, the
procedure is completed. If not, repeat steps 12 through 15.
16. Tighten the locking screws and record the setting for future
reference.
17. Perform PMT Dynode Voltage Verification/Adjustment
VP-22.
18. Perform WBC Gain Verification/Adjustment VP-23.
19. Perform WBC OPTI-CAL Verification/Adjustment (VP-24).
20. Perform NOC Gain Adjustment VP-25.
21. Perform RBC/PLT 0o Gain Verification/Adjustment VP-26.
22. Perform RBC/PLT 10o Gain Verification/Adjustment
VP-27.
Figure 5-4 CELL-DYN 3200 Optics Bench

R ear M irror
X A xis
Laser S trap Laser S trap
5 M illiw att H E N E Laser

Vertical P olarization
632.8 N anom eters
.75" 90 oD epolarized P M T
P lace B e am splitter/ 90 oP M T
P olarizer here during B eam sp litter/P olarizer
Laser po larization. Y
X
Y
P lace P o w er M eter
A H ead he re during 90 oS lit/F ield Lens
x Laser po larization. M irror R e ar View
i
s
F ixture H oles
90 oO bjective Lens
C ylinderc al Lens 10 o D etector
P erforate d M irror M ount

Tooling
F orw ard S lit Im aging Lens
H ole
F low C ell 0 oD etector
Y -A xis A lignm ent M ic. O bscura tion B ar

F orw ard S catter A ssy.
F ront M irror X -A xis A lignm ent M ic.
3 2 078
8#0#9HULILFDWLRQ#3URFHGXUHV VP-21 Optical Flow Cell Coarse Alignment
VP-21 Optical Flow Cell Coarse Alignment 5. Continue rotating CCW until the pattern of the front wall is
observed, and record the micrometer setting (Figure
'$1*(5=#/$6(5#/,*+7 Avoid direct 5-5-C).
exposure to laser beam. Refer to Introduction Figure 5-5 Beam Images
Section 1.7 for detailed safety information.
"A " "B "
C lear Q u artz R ear W all
7.00 Diameter Beads
S catter P attern
CELL-DYN 3200 Diluent/Sheath O bscuration B ar
Clean Container "C " "D "

F ront W all F low C ell C entered
1. Prepare the Bead solution. 320 79
B rightest Im age
b. Add 15 drops of 7.00 beads and mix well.
6. Rotate the micrometer CW to a setting half-way between
2. Remove top cover and flow cell access cover. those recorded in steps 3 and 4. This should coarsely
3. Loosen the locking screw on the lower (y-axis) center the flow cell channel on the beam; the pattern will
micrometer, and back off (CW viewed from top) on the resemble that of Figure 5-5-D and will be at the brightest
micrometer (Figure 5-5) until the beam is striking the flow level.
cell at a point to the rear of the flow cell channel. The 7. Cover the opening with black cloth to block ambient light.
scatter pattern on the obscuration bar should resemble
that in Figure 5-5-A. Placing a piece of white paper in front 8. From Main Menu, press
of the obscuration bar will aid in viewing the scatter DIAGNOSTICS
pattern. MORE
4. While observing the scatter pattern, rotate the micrometer WBC DATA
CCW until the pattern of the rear wall of the flow cell
channel is observed, and record the micrometer setting 9. Press
(Figure 5-5-B). CALC CV
8#0#9HULILFDWLRQ#3URFHGXUHV VP-22 PMT Dynode Voltage Verification/Adjustment
10. Place Beads solution under probe and press START 2. From Main Menu, press
SWITCH to aspirate Beads and run a count cycle. DIAGNOSTICS
11. If there is data in both displays, perform the fine alignment MORE
procedure VP-20. If there is no data in either display,
WBC DATA
Repeat steps 5 through 9.
3. Press
VP-22 PMT Dynode Voltage CALC CV
Verification/Adjustment 4. Place Beads solution under probe and press START
'$1*(5=#/$6(5#/,*+7 Avoid direct
5. Observe the mean channel values for the 90o and 90o
exposure to laser beam. Refer to Introduction
depolarized channels, and refer to Table 5-4. If the
displayed mean channel values are within specifications
and the recorded gain settings are less than 2500, the
Required Tools & Materials: dynode voltages need not be adjusted and the procedure
is completed. If either of the above are out-of-range, the
dynode voltage(s) for the channel(s) must be adjusted.
3/16 Allen Wrench
6. Remove the top cover from the analyzer, and locate the
7.00 Diameter Beads, PN 1606004 PMT preamplifiers on the right side of the optical bench.
CELL-DYN 3200 Diluent/Sheath The board on the left is for the 90o channel and the board
on the right is for the 90o depolarized channel. R11 adjusts
Clean Container
the dynode voltage on both boards.
Verification Procedure: 7. Press
1. Prepare the WBC Beads solution: MORE

a. Place 2 mL of diluent in a clean container. to first Diagnostics Screen, and press
b. Add 15 drops of 7.00 beads and mix well. F12
F1
SET POINT ENTRY
8#0#9HULILFDWLRQ#3URFHGXUHV VP-23 WBC Gain Verification/Adjustment
8. Move cursor to WBC 90D, enter 2000, and press 14. Press
ENTER. Move cursor to WBC 90DP, enter 2000, and DIAGNOSTICS
press ENTER.
MORE
9. Press
VOLTAGE READINGS
SET ANALYZER
15. Verify that both dynode voltages are less than 9.00 (900
RETURN volts). Dynode voltages greater that 900 volts indicate a
RETURN problem, and the problem must be isolated and corrected
MORE TO before continuing.
WBC DATA
VP-23 WBC Gain Verification/Adjustment
10. Cover optical bench with black cloth to block ambient light.
The following procedure verifies, and if necessary, adjusts the
11. Place the 7.00 beads solution under the probe, and gain of the WBC channels using 7.00 beads as a reference
press material.
EXTENDED WBC COUNT
There are two modes available for adjusting the WOC gain -
START ENTER SETTING and AUTO GAIN ADJUSTMENT.
to aspirate the Beads and start the cycle.
In the ENTER SETTING mode the proper gain setting is
o
NOTE: Both 90 channels are extremely sensitive to calculated manually and then entered. Use the following
ambient light. The black cloth must remain in place when equation when using this mode.
adjusting the dynode voltages. Desired Mean Channel
New Gain Setting = X Present Setting
12. Observe mean channel value on right display and adjust Present Mean Channel
R11 on 90o preamplifier (left PCB) until the displayed
values approximate the specifications shown in Table 5-1. In the AUTO GAIN ADJUSTMENT mode Beads is run on the
(Note that this is a coarse preliminary manual adjustment analyzer and the new gain setting is calculated and entered
that will be more finely tuned with the auto gain circuitry.) automatically. We will use this mode, if necessary, in the
following procedure.
13. Repeat steps 11 and 12 for the 90o depolarized
preamplifier (right PCB).
7.00 Diameter Beads
8#0#9HULILFDWLRQ#3URFHGXUHV VP-24 WBC OPTI-CAL Verification/Adjustment
Clean Container 8. Press

AUTO GAIN ADJUSTMNT
WBC GAINS
1. Prepare the WBC Beads solution: 9. If necessary, change the Mean Channel Numbers to the
a. Place 2mL of diluent in a clean container. desired values (Table 5-1) and press ENTER.
b. Add 15 drops of 7.00 beads and mix well. NOTE: You must run Beads twice in order to set gains.
2. From Main Menu, press The first run is needed to calculate new settings, and the
second is to verify the settings.
DIAGNOSTICS
MORE 10. Place Beads solution under probe, press
GAIN ADJUSTMNTS START
3. Press and press the START SWITCH to aspirate Beads and

run a count cycle.
VERIFY GAINS
WBC GAINS
SWITCH to aspirate Beads and run a count cycle to
4. If necessary, change the Mean Channel Numbers to the verify.
desired values (Table 5-1) and press
12. Press
ENTER
Y
5. Place Beads solution under probe, press ENTER
START to save new gain settings.
and press the START SWITCH to aspirate Beads and
run a count cycle.
VP-24 WBC OPTI-CAL Verification/Adjustment
6. If the Mean Channel Numbers are within specifications,
The following procedure verifies, and if necessary, adjusts the
skip steps 7 through 12.
gain of the WBC 90o and 90oD channels using OPTI-CAL as a
7. Press reference material. It also determines and stores the Beads
reference channels to be used for reference after an OPTI-CAL
RETURN
gain adjustment.
to access AUTO GAIN ADJUSTMNT
8#0#9HULILFDWLRQ#3URFHGXUHV VP-24 WBC OPTI-CAL Verification/Adjustment
Required Tools & Materials: 9. Press

7.00 Diameter Beads, PN 1606004 CELL REGION
CELL-DYN 3200 Diluent/Sheath 10. Change window settings to conform to the OPTI-CAL
Clean Container assay sheet.
OPTI-CAL 11. Press

RETURN
Verification/Adjustment Procedure: 12. Toggle the BLOOD/BEADS key to blood.
1. Insure Channels 1 through 4 offset voltages are less than 13. Run the OPTI-CAL 3 times and record the mean channel
2 volts. values for GRANs on 90o and 90oD on each run.
2. Insure Laser power is greater than 5mw and less than 14. Compute the average mean channel for 90o and 90oD for
7mw. the three runs.
3. Insure Channel WOC gain settings are less than 2500. 15. Compare the average to the Gran mean channel location
4. Insure Dynode voltages are less than 900 volts. given in the OPTI-CAL assay sheet. If it is within
specifications, skip steps 16 through 20.
5. Insure the CVs on all 4 channels are: 0o < 3.5%, 10o <
3.5%, 90o < 12.5%, 90oD < 12.5%. 16. Calculate the new gain values for 90o and 90oD, use the
following equation:
6. Insure the Auto Gain function has been run and the gains
have been set to the following: X (Y/Z)
0o = 35 +/- 3, 10o = 65 +/- 5. Where:
7. From Main Menu, press X = Current Gain Value.
DIAGNOSTICS Y = Mean Channel Value from Assay Sheet.

F12 Z = Current Mean Channel from Step 14.
F1 17. Press
8. Press RETURN
BEADS/BLOOD COMPARE SET POINT ENTRY
8#0#9HULILFDWLRQ#3URFHGXUHV VP-25 NOC Gain Adjustment
18. Enter the new value for 90o gain and press 26. Press
ENTER MORE
19. Enter the new value for 90oD gain and press GAIN ADJUSTMNTS
ENTER 27. Press
SET ANALYZER AUTO GAIN ADJUSTMNT
RETURN WBC GAINS
20. Press 28. Enter the Beads Channel numbers for 90o and 90oD.
BEADS/BLOOD COMPARE 29. Run an Auto Gain using Beads to enter these channel
and repeat steps 12 through 15. locations into the default table.
21. Toggle BLOOD/BEADS key to BEADS. 30. Reboot system on a Setup Disk. Perform a GET to store
these values.
22. Prepare the Bead solution.
VP-25 NOC Gain Adjustment
b. Add 15 drops of 7.00 beads and mix well.
The NOC gains are not calibrated with beads because the 0o
23. Run the Beads three times and record the Mean Channel and 10o channels are used for both WOC and NOC counting.
for 90o and 90oD on each run. Verify that 0o and 10o Mean However, the NOC gains must be higher than those used for
Channels are 35+/-3 and 65+/-5 respectively and all CVs WOC by a factor of 1.5. In this procedure, we simply multiply
are within specification on each run. the WOC 0o and 10o gains by 1.5 and change the NOC 0o and
24. Compute the average Mean Beads Channel for 90o and 10o setpoints to the new values.
90oD for the three runs.
NOTE: These Beads Channel locations are now specific
to this analyzer and must be entered into the AUTO GAIN None
table.
25. Press
RETURN
RETURN
8#0#9HULILFDWLRQ#3URFHGXUHV VP-26 RBC/PLT 0 Gain Verification/Adjustment
Verification/Adjustment Procedure: VP-26 RBC/PLT 0 Gain

1. From Main Menu, press Verification/Adjustment
DIAGNOSTICS The following procedure verifies and, if necessary, adjusts the
F12 gain of the RBC/PLT 0o channel using Beads spheres as a
reference material.
F1
2. Press Required Tools & Materials
SET POINT ENTRY 7.00 Diameter Beads, PN 1606004
3. Move the cursor to the NOC 0o setpoint. CELL-DYN 3200 Diluent/Sheath
4. Multiply the displayed WOC 0o setpoint by 1.5, change the Red-Top Vacutainer
NOC 0o setpoint to the new calculated value, and press Transfer Pipette
ENTER
5. Move the cursor to the NOC 10o setpoint. Verification/Adjustment Procedure:
6. Multiply the displayed WOC 10o setpoint by 1.5, change 1. Prepare the 7.00 beads solution:
the NOC 10o setpoint to the new calculated value, and a. Mix the 7.00 beads by shaking vigorously.
press b. Add 15 drops of 7.00 beads to a vacutainer.
ENTER c. Add 2 ml of 3200 Diluent/Sheath reagent.
7. If necessary, move the cursor to the NOC Lo threshold, 2. From Main Menu, press
change it to 400, and press
DIAGNOSTICS
ENTER
MORE
8. Press
GAIN ADJUSTMNTS
SET ANALYZER
3. Press
to store the new values.
VERIFY GAINS
9. Press RBC/PLT 0O GAIN
RETURN
RETURN
8#0#9HULILFDWLRQ#3URFHGXUHV VP-27 RBC/PLT 10 Gain Verification/Adjustment
4. If necessary, change the Mean Channel Number to the 11. Place Beads solution under probe and press START
desired value and press SWITCH to aspirate Beads and run a count cycle to
ENTER verify.
5. Place Beads solution under probe, press 12. Press
START Y
and press the START SWITCH to aspirate Beads and ENTER
run a count cycle. to save new gain setting.
6. If the Mean Channel Number is within specifications (180
+/- 1), skip steps 7 through 12. VP-27 RBC/PLT 10 Gain
7. Press Verification/Adjustment
RETURN The following procedure verifies and, if necessary, adjusts the
gain of the RBC/PLT 10o channel using Beads spheres as a
to access AUTO GAIN ADJUSTMNT.
reference material.
8. Press
AUTO GAIN ADJUSTMNT Required Tools & Materials:
RBC/PLT 0O GAIN 3.29 or 3.31 Beads, PN 1606005
9. If necessary, change the Mean Channel Number to the CELL-DYN 3200 Diluent/Sheath
desired value and press Red-Top Vacutainer
ENTER Transfer Pipette
NOTE: You must run Beads twice in order to set gain. The
first run is needed to calculate new setting, and the second Verification/Adjustment Procedure:
is to verify the setting.
1. Prepare the 3.29 or 3.31 Beads solution.
10. Place Beads solution under probe, press a. Mix the Beads by shaking vigorously.
START b. Add 10 drops of Beads and 20 drops of diluent to a
and press the START SWITCH to aspirate Beads and vacutainer and mix well.
run a count cycle.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-28 MAM 0 & 10 Log Amplifier Verification
2. From Main Menu, press NOTE: You must run Beads twice in order to set gain. The
DIAGNOSTICS first run is needed to calculate new setting, and the second
is to verify the setting.
MORE
10. Place Beads solution under probe, press
GAIN ADJUSTMNTS
START
3. Press
and press the START SWITCH to aspirate Beads and
VERIFY GAINS run a count cycle.
RBC/PLT 10O GAIN
4. If necessary, change the Mean Channel Number to the SWITCH to aspirate Beads and run a count cycle to
desired value and press verify.
ENTER 12. Press
5. Place Beads solution under probe, press Y
START ENTER
and press the START SWITCH to aspirate Beads and to save new gain setting.
run a count cycle.
6. If the Mean Channel Number is within specifications VP-28 MAM 0 & 10 Log Amplifier Verification
channel (120 +/- 1 for 3.29 or 121+/- 1 for 3.31), skip steps
7 through 12. The following procedure verifies the dynamic range of the 0o
and 10o log amplifiers on the MAM by running the 3 Beads and
7. Press calculating the ratio of the peak channels of the three sizes.
RETURN The CELL-DYN 3200 uses the 0o channel for and MCV, and the
to access AUTO GAIN ADJUSTMNT. 10o channel for RBCs, PLTs, and MPV, with the log amplifiers
used for both outputs. The 0o channel gain is set with 7.00
8. Press
beads by placing the peak channel in 180 +/- 1, and the lower
AUTO GAIN ADJUSTMNT range is verified with a ratio to a 4.99 RBC 0o Bead peak
RBC/PLT 10O GAIN. channel number. The 100 gain is set with 3.31 or 3.29 Beads by
placing the 3.29 peak channel in 120 +/- 1 or the 3.31 peak
9. If necessary, change the Mean Channel Number to the channel in 121 +/- 1, and the upper range is verified with a ratio
desired value and press to a 7.00 beads RBC 10o peak channel number.
ENTER
The following table and equations are an example of a typical Transfer Pipette
RBC and PLT verification/adjustment.
Verification/Adjustment Procedure:
Table 5-5 0o & 10o Log Amplifier Verification
1. Prepare the 7.00 beads solution:
RBC 0o RBC 10o
Beads PLT 0o P.C. PLT 10o P.C. a. Mix the 7.00 beads by shaking vigorously.
P.C. P.C.
3.29 N/A N/A N/A Set at 120 b. Add 15 drops of 7.00 beads to a vacutainer.
Falls at c. Add 2 ml of 3200 Diluent/Sheath reagent.
4.99 N/A N/A N/A
129 2. In the RUN menu, press
Set at SPECIMEN TYPE
7.00 Falls at 200 N/A N/A
180
BEADS
RETURN.
0o Equation:
7.00 RBC 0o P.C. = 1.400.04 180 = 1.39 (within spec) DIAGNOSTICS
4.99 RBC 0o P.C. 129 MORE
RBC DATA
o
10 Equation: 4. Turn smoothing off, place Beads solution under probe,
7.00 RBC 10o P.C. = 1.670.04 200 = 1.66 (within spec) and press the START SWITCH to aspirate Beads and
3.xx PLT 10o P.C. 120 run a count cycle.
5. When cycle is completed, view RBC 0 DATA and RBC 0
HISTOGRAM, and verify the peak is in channel 180 +/- 1
Required Tools & Materials: channel. If the RBC 0o peak channel number is within
7.00 Diameter Beads, PN 1606004 specifications, record the RBC 0o and RBC 10o peak
channel numbers, and skip step 6.
3.29 or 3.31 Beads, PN 1606005
4.99 Beads, PN 1606003 (VP-26), and repeat steps 3 through 5.
7. Prepare the 4.99 beads solution:
Red-Top Vacutainer
a. Mix the 4.99 beads by shaking vigorously.
b. Add 15 drops of 4.99 beads to a vacutainer. 15. Plug the appropriate 0o peak channel numbers into the
following equation, and calculate the 0o log amplifier ratio.
8. Place the Beads under probe, and press the START
If the ratio is out-of-specifications, the 0o log amplifiers
must be adjusted (VP-29) or the MAM must be replaced.:
9. When cycle is completed, view RBC 0 DATA and RBC 0
HISTOGRAM and record the RBC 0o peak channel 0o Equation:
number 7.00 RBC 0o P.C. = 1.40 0.04
10. Prepare the 3.29 or 3.31 Beads solution: 4.99 RBC 0o P.C.
a. Mix the Beads by shaking vigorously. 16. Plug the appropriate 10o peak channel numbers into the
b. Add 10 drops of Beads and 20 drops of diluent to a following equation, and calculate the 10o log amplifier
vacutainer and mix well. ratio. If the ratio is out-of-specifications, the 10o log
amplifiers must be adjusted (VP-30) or the MAM must be
11. Press
replaced.
DIAGNOSTICS
PLT DATA
PLT 10 DATA. 10o Equation:
7.00 RBC10o P.C. = 1.67 0.04
12. Turn smoothing off, place Beads solution under probe,
and press the START SWITCH to aspirate Beads and 3.xx PLT10o P.C.
run a count cycle.
13. When cycle is completed, view PLT 10 DATA and PLT
10 HISTOGRAM, and verify the peak is in channel (120
+/- 1 for 3.29 or 121+/- 1 for 3.31). If the PLT 10o peak
channel number is within specifications, record the PLT 10
peak channel number, and skip step 14.
(VP-27), and repeat steps 12 and 13.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-29 0 MAM Log Amplifier Verification/Adjustment
VP-29 0 MAM Log Amplifier Required Tools & Materials:

Verification/Adjustment Voltage-Divider Fixture, PN 9450202
The following procedure verifies and, if necessary, adjusts the Digital Voltmeter
0o log amplifier gains and offsets on the MAM. Refer to Figure Potentiometer Adjustment Tool or Small Slotted
5-6. A voltage-divider is used to inject a 10mv reference voltage Screwdriver
into the 0o log amplifier, and gains and offsets are adjusted to
provide the initial setup of the 0o log amplifier chain.
Verification/Adjustment Procedure:
Figure 5-6 Voltage-Divider Fixture
1. Refer to Figure 5-6. Install the fixture on the MAM by
R ed (+1 5V )
attaching the red lead to the top of L2, black lead to TP7,
and the yellow lead to TP8.
5K O H M S
2. Attach the positive lead of the DVM to TP8 and the
negative lead to TP7.
100 O H M S Yellow (+10m v) 3. Adjust the pot on the fixture for a reading of +10.0mv +/-
0.2mv.
3 2 0 80
B lack (G N D ) 4. Move the positive lead to TP11, and adjust R102 for
-23.0mv +/- 0.2mv
Figure 5-7 MAM Test Points & Potentiometers
TP 2
R84
3 2 0 81
R102 TP 11 R106
TP 9
L2
TP 7
TP 1 7 TP 1 8
R137
TP 8 R132 R131
R138 TP 2 1
TP 1 5 TP 1 6
5. Move the positive lead to TP15, and adjust R132 for

-1.000V +/- 0.010V.
6. Move the positive lead to TP16, and adjust R137 for 3.68V
+/- 0.010V.
7. Leave meter as is, and adjust R132 for a reading of 1.72V
+/- 0.010V.
NOTE: After performing this procedure the 0o gain must

be verified/adjusted with Beads (VP-26).
VP-30 10 MAM Log Amplifier 3. Adjust the pot on the fixture for a reading of +10.0mv +/-
0.2mv.
4. Move the positive lead to TP18, and adjust R106 for
The following procedure verifies and, if necessary, adjusts the
-10.0mv +/- 0.2mv
10o log amplifier gains and offsets on the MAM. Refer to Figure
5-8. A voltage-divider is used to inject a 10mv reference voltage 5. Move the positive lead to TP21, and adjust R131 for
into the 0o log amplifier, and gains and offsets are adjusted to -1.000V +/- 0.010V.
provide the initial setup of the 10o log amplifier chain.
6. Move the positive lead to TP9, and adjust R138 for 4.20V
Figure 5-8 Voltage-Divider Fixture +/- 0.010V.
R ed (+1 5V ) 7. Leave meter as is, and adjust R131 for a reading of
0.750V +/- 0.010V.
5K O H M S
NOTE: After performing this procedure the 10o gain must

be verified/adjusted with Beads (VP-27).
100 O H M S Yellow (+10m v)
3 2 0 80 VP-31 Aspirate/Vent Needle Verification

B lack (G N D )

None
Voltage-Divider Fixture, PN 9450202
Digital Voltmeter
DIAGNOSTICS
Potentiometer Adjustment Tool or Small Slotted
Screwdriver MORE
TOWER TEST
Verification/Adjustment Procedure: 2. Press
1. Refer to Figure 5-6. Install the fixture on the MAM by NEEDLE DOWN
attaching the red lead to the top of L2, black lead to TP7,
3. Verify that the needle moves down smoothly without any
and the yellow lead to TP17
stopping or jerking. Also verify that the GS1 sensor status
2. Attach the positive lead of the DVM to TP17 and the displays the following:
negative lead to TP7.
GS1 UP:SENSOR STATUS
8#0#9HULILFDWLRQ#3URFHGXUHV VP-32 GS2 Stop Solenoid Verification
GS1=1 VP-33 Bar Code Spin Assembly Verification

GS1 DOWN:SENSOR STATUS
GS1=0
None
4. Press
NEEDLE UP Verification/Adjustment Procedure:
5. Verify that the needle moves up smoothly without any 1. From Main Menu, press
stopping or jerking.
DIAGNOSTICS
MORE
VP-32 GS2 Stop Solenoid Verification
TOWER TEST
Required Tools & Materials: 2. Place a tube at the read station, and press
None SPIN MOTOR
3. Verify that the tube is spun smoothly without any stopping
Verification/Adjustment Procedure: or jerking.
1. From Main Menu, press 4. Press
DIAGNOSTICS STOP MOTOR
MORE to return to DIAGNOSTICS.
TOWER TEST
2. Alternately press Retract Solenoid and Extend VP-34 Tube Height Sensors (S1/S2)
Solenoid. Verification
3. Verify that the GS1 solenoid retracts and extends This diagnostic routine first moves GS1 and GS2 down and
smoothly and fully with each command. Also verify that then up in the same manner as a count cycle. It then displays
LED DS1 on SHM1 is on with the retract command and off the status of sensors GS1, S1, and S2 in both the up and down
with the extend command. positions (1=sensor blocked, 0=sensor unblocked). It also
displays the type of tube sensed at the aspirate position: BD,
SST, or No Tube.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-35 Mixer Up/Down Verification
Required Tools & Materials: GS1=0 S1=1 S2=1SST

BD Specimen Tube No Tube Example:
Sarstedt Specimen Tube GS1 UP: Sensor Status
Verification/Adjustment Procedure: GS1=1 S1=0 S2=0
1. From Main Menu, press GS1 Down: Sensor Status Tube Type
DIAGNOSTICS GS1=0 S1=0 S2=1 No Tube
MORE
TOWER TEST VP-35 Mixer Up/Down Verification
2. Place the appropriate tube at the vent/aspirate position.
3. Press
None
SENSOR TEST
GS1 DOWN Verification/Adjustment Procedure:
4. Verify the following sensor status and tube type for each 1. From Main Menu, press
tube condition.
DIAGNOSTICS
BD Tube Example:
MORE
GS1 UP: Sensor Status LOADER TEST
GS1=1 S1=0 S2=0 2. Alternately press MIXER DOWN and MIXER UP and
verify that the assembly moves up and down smoothly
GS1 Down: Sensor Status Tube Type
without any stopping or jerking. Also verify that the home
GS1=0 S1=1 S2=0BD sensor is 1 when up and 0 when down.
Sarstedt Tube Example:

VP-36 Mixer Head Rotation Verification
GS1 UP: Sensor Status
GS1=1 S1=0 S2=0 Required Tools & Materials:
GS1 Down: Sensor Status Tube Type None
8#0#9HULILFDWLRQ#3URFHGXUHV VP-37 Mixer Bladders Verification
Verification/Adjustment Procedure: VP-38 Rack Advance & Tube Sensors

1. From Main Menu, press Verification
DIAGNOSTICS
MORE
LOADER TEST Specimen Tubes
Specimen Loader Rack
2. Alternately press ROTATE MIX UP and ROTATE MIX
DOWN and verify that the mixer head rotates up and
down smoothly without any stopping or jerking. Also verify Verification/Adjustment Procedure:
that the home sensor is 0 when up and 1 when down. 1. From Main Menu, press
DIAGNOSTICS
VP-37 Mixer Bladders Verification
MORE
LOADER TEST
2. Place a rack with alternating tubes at the load side rear
None
wall.
Verification/Adjustment Procedure: 3. Press
1. From Main Menu, press START RACK ADVANCE
DIAGNOSTICS 4. Verify that the rack indexes smoothly through the

processing station without any stopping or jerking during
MORE
index movements.
LOADER TEST
5. Verify that when a tube is present at a sensor position a 1
2. Press is indicated, and when a tube is not present a 0 is
CYCLE BLADDERS indicated.
3. Alternately press GRIP TUBE and RELEASE TUBE
and verify that the mixer bladders inflate and deflate
completely, and that there are no pressure or vacuum
leaks.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-39 Cross Transfer Arms & Rack Sensors Verification
VP-39 Cross Transfer Arms & Rack Sensors 5. Remove rack from the load side, place five racks in the
unload side, and press
Verification
EXTEND ARMS
Required Tools & Materials: Verify the following sensor status, and press RETRACT
ARMS.
5 Racks
SENSOR STATUS
Verification/Adjustment Procedure: EXTEND ARMS:LOAD SENSOR = 1
1. From Main Menu, press UNLOAD FULL =1
DIAGNOSTICS
UNLOAD WARN = 1
MORE
LOADER TEST VP-40 Rack Bar Code Labels Verification
2. Leave the load side and unload side empty, and
alternately press EXTEND ARMS and RETRACT Required Tools & Materials:
ARMS.
5 Racks
3. Verify that the arms extend and retract smoothly without
any stopping or jerking. Verification/Adjustment Procedure:
4. Place one rack in the load side, leave unload side empty, 1. From Main Menu, press
and press
DIAGNOSTICS
EXTEND ARMS
MORE
Verify the following sensor status, and press
VER RACK BAR CODE
RETRACT ARMS
2. Manually move a rack through the processing station,
SENSOR STATUS positioning the rack and tube position bar code labels at
EXTEND ARMS:LOAD SENSOR = 0 the slot in the processing station.
UNLOAD FULL =0 3. Verify that the bar code number on the screen matches the
number on the rack.
UNLOAD WARN = 0
8#0#9HULILFDWLRQ#3URFHGXUHV VP-41 Mixer Bladders Pressure Verification/Adjustment
VP-41 Mixer Bladders Pressure 7. Adjust pressure regulator (just to right of load side empty
sensor) for 6.5 psi +/- 0.2 psi.
8. Reconnect tubing to vacuum/pressure port.
Pressure Gauge VP-42 Shear Valve Driver Lubrication
Slotted Screwdriver Procedure
Verification/Adjustment Procedure: Required Tools & Materials:

1. From Main Menu, press Oil PN 14237-015 (Dallas/Europe), 1605806 (Santa Clara)
DIAGNOSTICS
MORE
1. Power off instrument using the proper shutdown
VOLTAGE READINGS
procedure.
2. Verify Press 1 psi is 12.5-13.5 psi. If out-of-range,
2. Remove the shear valve driver from the Analyzer.
perform Vacuum & Pressure Level Verification/Adjustment
(VP-16). 3. Lubricate the assembly as shown in Figure 5-9.
3. Press 4. Re-install the shear valve driver and verify instrument
MORE performance.
LOADER TEST
4. Press
CYCLE BLADDERS
5. Remove tubing from vacuum/pressure port on mixer head,
and connect tubing to pressure gauge.
6. Press
GRIP TUBE
to apply pressure.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-43 Optics Bench Cleaning Procedure
Figure 5-9 Shear Valve Driver Lubrication Points
2-3 D rop s O f O il 3 P lace s
F ront
3 2 0 82
2-3 D rop s O f O il 2 P lace s
VP-43 Optics Bench Cleaning Procedure Verification Procedure:
'$1*(5=#/$6(5#/,*+7 Avoid direct 1. Clean the left side and rear fan filters.
exposure to laser beam. Refer to Introduction 2. Remove Analyzer top cover and plastic optics covers.
3. Close Laser Shutter.
Required Tools & Materials: 4. Using pressurized air, remove dust and dirt from optics
Cotton Swabs bench and surrounding area.
High Grade Lens Paper (Kodak recommended - DO NOT
USE Baxter S/P, as it may scratch the lens.)
Canned Air - Optics Grade
5. Using Kodak lens paper on the end of a cotton swab, 9. Check for a red glow or halo around the forward slit. If
clean rear mirror, cylindrical lens, front mirror, imaging laser output lens dirty, a halo will appear around football
lens, and flow cell. shaped laser beam. Refer to Figure 5-11.
&$87,21=##Do not use lens cleaning solution on Figure 5-11 Forward Slit Halo
mirrors. If cleaning fluid is needed, use reagent grade
methanol only. F orw ard
S lit
F ootball
6. Repeat cleaning process to ensure all residual particles S haped
are removed. B eam
7. Open Laser Shutter.

8. With laser on, check for a red glow or halo around opening
where laser beam exits the laser tube. Refer to Figure N O R ed
5-10. H alo
Figure 5-10 Laser Output Halo 3 2 0 84
Laser Tube
R ear
M irror
N O R ed
H alo
3 2 0 83
10. If halo exists in either location, clean laser with dry cotton Figure 5-13 Mark Mirror Positions
swab, or use lens paper. Refer to Figure 5-12.
Figure 5-12 Laser Tube Cleaning M ark
Location
Laser Tube
R ear
M irror
3 2 0 87
Insert C otton
S w ab H ere b. Remove screws and mirror. Refer to Figure 5-14.
3 2 0 85
Figure 5-14 Mirror Removal
11. Close Laser Shutter.

Tape
12. Clean 10o mirror.
a. Mark position of mirror and housing with pencil. Refer
to Figure 5-13. R em ove
M irror
3 2 0 88
5 - Verification Procedures VP-44 Optical Flow Cell Cleaning & De-Bubble Procedure
c. Clean mirror using reagent grade methanol or 16. Perform RBC/PLT 0o Gain Verification/Adjustment
microscope lens cleaning solution (green) and high (VP-26).
grade lens paper. (Kodak recommended - DO NOT
USE Baxter S/P). Ensure surface is clean; use
(VP-27).
compressed air to remove any remaining lint.
d. Replace mirror and align marks. DO NOT tighten
screws. VP-44 Optical Flow Cell Cleaning & De-Bubble
e. Open Laser Shutter. Procedure
13. Use the following procedure to adjust the mirror for DANGER: LASER LIGHT Avoid direct
maximum 10o mean. exposure to laser beam. Refer to Introduction
a. Prepare the WBC Beads solution by placing 2 mL of Section 1.7 for detailed safety information.
diluent in a clean container, adding 15 drops of 7.00
beads, and mixing well.
b. From Main Menu, press
20% Bleach or Abbott, ISE Conditioning Solution
n DIAGNOSTICS LN1360-02
n MORE
n WBC DATA 10 mL Syringe
c. Place the 7.00 beads solution under the probe, and Needle Nosed Pliers
press
n EXTENDED WBC Verification Procedure:
n COUNT 1. Fill syringe with 20% bleach solution or ISE Cleaning
n START Solution.
to aspirate the Beads and start the cycle. 2. From Main Menu, press
d. Observe the 10o mean, and rotate the mirror for the
n SPECIAL PROTOCOLS
highest 10o mean reading.
n EMPTY/FILL FLOW CELL
e. Tighten mirror screws ensuring maximum reading is
maintained, and erase pencil marks. n EMPTY FLOW CELL
14. Perform WBC Gain Verification/Adjustment (VP-23).

15. Perform NOC Gain Adjustment (VP-25).
8#0#9HULILFDWLRQ#3URFHGXUHV VP-44 Optical Flow Cell Cleaning & De-Bubble Procedure
3. From Main Menu, press Figure 5-15 Flow Cell Cleaning Plumbing
DIAGNOSTICS
MORE Flo w C ell D e-Bu b b le
P ro ce s s
SOLENOID OPERATION
4. To open solenoid 56, press
STEP SOLENOID
type 56, and press
ENTER
5. Refer to Figure 5-15. Remove elbow from right side of
solenoid 57, and connect elbow to syringe.
6. Inject bleach or cleaning solution in short strokes to create 56
turbulent motion in flow cell. In je c t 20 %

B le a ch o r
IS E C le an in g
7. Let solution remain is flow cell for three to five minutes. 57
S o lu tio n
& Air a t
Th is P o in t
&$87,21=##Do not allow ISE cleaning solution to remain in
91
flow cell for over five minutes. 3 2 0 89
8. Disconnect syringe, and connect reconnect elbow to

solenoid 57.
9. Initialize and prime instrument, and run three background
counts.
10. Verify that background counts are within specifications:
WBC 0.10 K/L, RBC 0.02 M/L, and PLT 5 K/L.
11. Refer to Figure 5-15. Verify that there are no large bubbles
in the flow cell. If large bubbles are present, perform
Optical Flow Cell Wetting Procedure (VP-43).
8#0#9HULILFDWLRQ#3URFHGXUHV VP-45 Optical Flow Cell Wetting Procedure
VP-45 Optical Flow Cell Wetting Procedure 6. Inject HGB lyse reagent in short strokes to create turbulent
motion in flow cell.
'$1*(5=#/$6(5#/,*+7 Avoid direct 7. Let lyse remain is flow cell for three to five minutes.
Section 1.7 for detailed safety information. 8. Disconnect syringe, and connect reconnect elbow to
solenoid 57.
Required Tools & Materials: 9. Initialize and prime instrument, and run three background
counts.
CN Free HGB/NOC Lyse
10 mL Syringe 10. Verify that background counts are within specifications:
Verification Procedure: 11. Refer to Figure 5-16. Verify that there are no large bubbles
in the flow cell. If large bubbles are present, repeat this
1. Fill syringe with HGB lyse solution. procedure.
2. From Main Menu, press Figure 5-16 Flow Cell Bubbles
SPECIAL PROTOCOLS To W a ste /
EMPTY/FILL FLOW CELL C e ll

Va cu u m
EMPTY FLOW CELL

S tre a m
N O L a rg e
3. From Main Menu, press Fe w S m a ll A ir A ir B u b b le s
B ub b le s a re O K
DIAGNOSTICS
MORE
SOLENOID OPERATION
4. To open solenoid 56, press
STEP SOLENOID
type 56, and press
ENTER S he a th P rim e
5. Refer to Figure 5-15. Remove elbow from right side of S he a th In

3 2 0 90
solenoid 57, and connect elbow to syringe. S am p le In
8#0#9HULILFDWLRQ#3URFHGXUHV VP-46 Laser Power Output Verification
VP-46 Laser Power Output Verification Figure 5-17 Laser Output Power Check
5 MW
'$1*(5=#/$6(5#/,*+7 Avoid direct Power
M e te r 32091
M o u n t C e n te re d
in B e a m Pa th
Laser Power Meter
Optical Alignment Tool Kit, PN 8921183801
1. Ensure laser has been on for at least 15 minutes.
2. Perform Optics Bench Cleaning Procedure (VP-43).
3. Refer to Figure 5-17. Check laser power after first mirror: 4. Refer to Figure 5-18. Check laser power after forward slit:
a. Mount power meter photodiode centered in beam path. a. Mount power meter photodiode centered in beam path
b. Verify that power is 5 mW or greater. between forward slit and imaging lens.
c. If power is less than 5 mW, replace laser tube. b. Power meter should read at least 1/5 of that obtained
in step 3.
5. Perform Optical Offsets Verification (VP-47).
8#0#9HULILFDWLRQ#3URFHGXUHV VP-47 Optical Offsets Verification
Figure 5-18 Laser Power At Forward Slit

3. Verify that CH 1 through CH4 Offsets are less than 1.00
volt.
4. If any are greater than 1.00 volts, perform Optics Bench
Cleaning Procedure (VP-43).
VP-48 Shear Valve Cleaning Procedure

DI Water
P ow e r
M e ter
Clean Container
1 /5 o f P o w e r
o f First M irro r Lint Free Wipes
3 2 0 92
SPECIAL PROTOCOLS
VP-47 Optical Offsets Verification CLEAN/RES SHEAR VAL
CLEAN SHEAR VAL
2. Refer to Figure 5-19. Unscrew shear valve knob.
Optical Alignment Tool Kit, PN 8921183801
&$87,21=##Do not drop center ceramic section.
DIAGNOSTICS 3. Remove three ceramic sections from shear valve driver
MORE leaving tubing in place, and place center ceramic piece in
a container of DI water.
VOLTAGE READINGS
4. Use damp lint free wipes to clean front and rear surfaces
2. Ensure top cover is in place, or cover optics bench with of front and rear ceramic sections.
black cloth.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-49 HGB Flow Cell Cleaning Procedure
5. Clean center mounting holes in front and rear ceramic VP-49 HGB Flow Cell Cleaning Procedure
sections.
This procedure first uses the Auto Clean routine to clean the
Figure 5-19 Shear Valve Disassembly flow cell, and if that is unsuccessful a manual procedure is
used.
Loop
Rear
S he a r Required Tools & Materials:
C e n te r
Va lv e
Fron t
B lo ck CELL-DYN Enzymatic Cleaner
Loop M o un tin g G u id e 20% Bleach Solution
K no b
R im N o tch 20 mL Syringe
L o ck N otc h 3 2 0 93
6. Clean mounting guide and lock notches in each ceramic SPECIAL PROTOCOLS
section.
MORE
7. Using a flashlight, inspect ports on front and rear ceramic AUTO CLEAN
sections, and ensure no foreign material blocks ports.
Follow the on-screen instructions to perform the
8. Remove center section from DI water, and clean both procedure.
sides to remove any blood or foreign material.
2. Run three background counts to purge enzyme cleaner.
9. Wet all surfaces with DI water and assemble as shown in
Figure 5-19. Ensure wide notches are down and rim notch 3. From Main Menu, press
on center section is to the right. DIAGNOSTICS
10. Tighten shear valve knob until it stops. MORE
VOLTAGE READINGS
11. Press
RESTORE SHEAR VAL 4. Verify that the HGB OUTPUT voltage is 5.00 volts
+0.2/-0.00 volts. If the voltage is within range, skip steps 5
12. Run background counts until all are within specifications: through 13.
5. Manually open solenoid 93 to drain liquid from flow cell.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-50 Open Mode Aspiration Path Cleaning Procedure
6. Prepare and aspirate 20% bleach solution into syringe. 13. Verify that the HGB OUTPUT voltage is 5.00 volts
+0.2/-0.00 volts. If voltage is slightly out-of-range, perform
7. Refer to Figure 5-20. Remove tubing from port and
HGB Current Verification/Adjustment (VP-5).
connect syringe to port.
8. Inject bleach solution until it can be seen exiting top port, VP-50 Open Mode Aspiration Path Cleaning
and let remain in flow cell for five to ten minutes.
Procedure
9. Manually open solenoid 93 to drain bleach from flow cell.
:$51,1*=#327(17,$/#%,2+$=$5' The
10. Remove syringe, and connect tubing to port.
aspiration needle/probe are sharp and potentially
11. Run three background counts to purge bleach. contaminated with infectious materials. Avoid
contact with the tips of the needle/probe.
Figure 5-20 HGB Flow Cell Plumbing

Inje ct B lea ch
To Here CELL-DYN Enzymatic Cleaner
R em ove Tu bing Specimen Tubes
& Co nnect Syringe
Wire Stylet, Gauge #23
Lint Free Tissues
H GB Flow Ce ll
Diluent
3 2 0 94
1. In the RUN mode use CHANGE SAMPLER to select
OPEN SAMPLER.
2. Manually open solenoid 12 to purge liquid from plumbing.
DIAGNOSTICS 3. Remove tubing from top of open probe.
MORE 4. Place a tissue under probe, run stylet into the top of probe
VOLTAGE READINGS until it extends out the bottom, and wipe any material from
stylet. Run up and down several times and remove.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-51 Closed Mode Aspiration Path Cleaning Procedure
5. Clean outside of probe several times with tissue 3. Manually open solenoid 12 to purge liquid from plumbing.
dampened with Enzymatic Cleaner, and reconnect tubing
4. Place a tube filled with enzyme cleaner at the aspirate
to top.
position, and press
6. Place a tube containing 4 mL of Enzymatic Cleaner under NEEDLE DOWN
probe, manually open solenoid 12, and close when
Enzymatic Cleaner passes through solenoid 12. 5. Manually open solenoid 12, and close when enzyme
cleaner passes through solenoid 12.
7. Let Enzymatic Cleaner remain for five minutes, and
manually open 12 until plumbing is emptied. 6. Manually hold solenoid 38 closed, and momentarily open
solenoid 36 to aspirate enzyme cleaner into vent needle.
8. Place a tube filled with diluent under probe, and open 12
until tube is emptied to flush plumbing. 7. Let enzyme cleaner remain for five minutes, press
9. Run background counts until all are within specifications: NEEDLE UP

WBC 0.10 K/ L, RBC 0.02 M/ L, and PLT 5 K/L. and manually open 12 until plumbing is emptied.
8. Initialize and Prime instrument.
VP-51 Closed Mode Aspiration Path Cleaning
9. Run background counts until all are within specifications:
Procedure WBC 0.10 K/L, RBC 0.02 M/L, and PLT 5 K/L.

CELL-DYN Enzymatic Cleaner
Specimen Tubes
1. In the RUN mode use CHANGE SAMPLER to select
CLOSED SAMPLER.
DIAGNOSTICS
MORE
TOWER TEST
8#0#9HULILFDWLRQ#3URFHGXUHV VP-52 Power-Up CPU/DCM 7-Segment LED Verification
VP-52 Power-Up CPU/DCM 7-Segment LED VP-53 Software Installation Procedure

Verification
Required Tools & Materials: CELL-DYN System Installation Disks 1 & 2 P.N. 9380590
None
Verification Procedure: 1. Turn system power off.
1. Power-up the system and observe the 7-segment LED on 2. Insert disk #1 in the floppy drive.
the CPU/DCM. Refer to the following table for status
conditions. 3. Turn system power on.
NOTE: After all the tests pass and solenoids energize, 1 4. Follow the on-screen instructions to install software.
and E will alternately flash in the display. This is a normal 5. At the C:\CD3200> prompt type A:INSTALL and press
condition and does not indicate a problem.
ENTER
Table 5-6 CPU/DCM 7-Segment LED Status Conditions 6. When prompted, install disk #2 and follow the on-screen
0 Indicates system power-up. directions.
1 Indicates that the CPU/DCM flash memory failed. 7. When installation is complete, remove disk #2 and
Indicates that the CPU/DCM first 524K of DRAM power-down and re-boot instrument.
2
failed.
Indicates that the CPU/DCM second 524K of VP-54 Hard Drive Verification
3
DRAM failed.
Indicates that the CPU/DCM third 524K of DRAM Required Tools & Materials:
4
failed. None
Indicates that the CPU/DCM fourth 524K of DRAM
5
failed. Verification Procedure:
6 Indicates that all CPU/DCM memory passed. 1. In the MAIN menu press
F12
F8
8#0#9HULILFDWLRQ#3URFHGXUHV VP-55 Floppy Drive Verification
2. At the C:\CD3200 prompt, type CHKDSK/F and press VP-55 Floppy Drive Verification
ENTER
3. At the selection prompt type Y and press Required Tools & Materials:
ENTER None
to run CHKDSK/F.
.
1 ,2 7 9 ,6 8 8 ,7 0 4 b y te s to ta l d is k sp a c e 1. In the MAIN menu, press
1 3 1,0 7 2 b y te s in 2 h id d e n file s F12
3 ,1 7 8 ,4 9 6 b yte s in 9 7 d ire cto rie s
F8
7 5 0 ,22 3 ,3 6 0 b y te s in 2 ,2 1 5 u s e r file s
5 2 6 ,1 5 5,7 7 6 b y te s a v a ila b le o n d is k 2. At the C:\CD3200 prompt, type A: and press
ENTER
3 2 , 7 6 8 b y te s in e a c h a llo c a tio n u n it
3 8 ,0 53 to ta l a llo ca tio n u n its o n d is k 3. At the A:\> prompt, type CHKDSK/F and press
16 ,0 5 7 a v a ila b le a llo ca tio n u n its o n d is k ENTER
6 5 5 ,3 6 0 to ta l b yte s m e m ory 4. At the selection prompt type Y and press
3 2 0 95
6 1 8 ,5 6 0 b yte s fre e ENTER
to run CHKDSK/F.The display should closely resemble
Figure 5-21 CHKDSK Results, Hard Disk Figure 5-22.
4. If lost allocation units are reported, type N to delete. 5. If there are no indications of a defect, type C: and press
5. The display should closely resemble Figure 5-22. If a line ENTER
is added saying xxxx bytes in bad sectors verify that 6. Then type rll and press
the number of bytes is less than 1% of the total disk space.
If the number exceeds 1%, the hard drive is suspect and ENTER
should probably be replaced. to return to the MAIN menu.
6. If there are no indications of a defect, type rll and press
ENTER to return to the MAIN menu.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-56 Background Counts Verification
Table 5-7 Background Count Specification

1 ,4 5 7 ,6 6 4 bytes total disk space
WBC
1 9 5 ,0 7 2 bytes in 4 user files NOC RBC HGB PLT
(WOC)
1 ,2 6 2 ,5 9 2 bytes available on disk
0.10 k/ L 0.10 k/L 0.02 m/L 0.1g/dL 5.0 k/L
5 1 2 bytes in each allocation unit
2 ,8 4 7 total allocation units on disk VP-57 System CV Verification
2 ,4 6 6 available allocation units on disk
6 5 5 ,3 6 0 total bytes memory Required Tools & Materials:

6 1 8 ,5 6 0 bytes free 2.5 mL Fresh Normal Blood
Figure 5-22 Floppy Drive CHKDSK Results Verification Procedure:

VP-56 Background Counts Verification RUN
SPECIMEN TYPE
2. Move the cursor to an empty file, and press
None
QC SPECIMEN
Verification Procedure: 3. Mix the fresh blood thoroughly, and run 10 times in the
open mode and 10 times in the closed mode.
4. Press
RUN
SPECIMEN TYPE MAIN
BACKGROUND QUALITY CONTROL
VIEW QC LOG
2. Press the TOUCH PLATE to run a background count.
5. Verify the CVs are within specification according to the
3. Verify that the background counts are within specifications
following table.
according to the following table.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-58 System Calibration Verification/Adjustment
Table 5-8 CV Specifications b. From MAIN MENU press

WBC CALIBRATION
NOC RBC HGB MCV PLT MPV AUTO-CALIBRATE
(WOC)
2.7% 2.7% 1.5% 1.0% 1.0% 4.0% 5.0% and toggle F2 to CALIBRATOR.
c. Enter the reference values from the assay sheet,
pressing [ENTER] after each entry.
VP-58 System Calibration
d. Press
START AUTO-CAL
Required Tools & Materials: to ready the instrument for calibration.
e. Prepare the calibrator for processing according to the
CELL-DYN Calibrator
assay sheet.
Verification Procedure: f. Run the calibrator 5 times, mixing well between runs.
g. Press
1. Pre-calibration checks:
ACCEPT
a. Verify reagent type, expiration date, and volumes are
at least 1/2 full. to enter the new factors for the parameters that need
calibration.
b. Verify calibrators are not expired.
c. Ensure background counts are within specifications
(VP-56).
VP-59 Vacuum Accumulator #1 Rinsing
d. Ensure WOC offsets for channels 1 through 4 are less
Procedure
than 1.00 volt.
e. Verify system precision is within specifications (VP-57). Required Tools & Materials:
Clean >250 mL Container
2. Perform Open Mode Auto Calibration using commercial
calibrator only. DI Water
NOTE: If any problems are encountered, refer to 0.125"ID 0.250"OD Tubing, 1 Foot
Auto-Cal Procedure-Open Mode in Chapter 6 of the #2 Phillips Screwdriver
CELL-DYN 3200 Operations Manual for a complete
description of the calibration procedure.
a. Ensure Analyzer in Open Mode.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-60 Vacuum Accumulator #2 Rinsing Procedure
Verification Procedure 5. Place tubing connected to position 4 into container, and

allow all DI water to be aspirated into Vacuum
1. Initialize Analyzer, and remove Left Side Cover. Accumulator #1.
2. Extend Air Supply out of Analyzer (Vacuum/Pressure 6. Remove tubing from position 4, connect original tubing,
Assembly (RR-7)). and unpinch position 7.
3. Measure 250 mL (1 cup) of DI water into a clean container. 7. Wait at least 5 minutes to remove any saline bridges
&$87,21=##Do not place more than stated amount of between electrodes.
DI water in container. 8. From Main Menu, press
DIAGNOSTICS
4. Refer to Figure 5-23. Pinch tubing at position 7 of MORE
manifold, remove tubing from position 4, and attach DRAIN ACCUMULATORS
0.125"ID 0.250"OD tubing to fitting at position 4.
9. Initialize Analyzer and repeat Step 8.
5P SI 12"H g 8"H g 12P SI 12"H g
VP-60 Vacuum Accumulator #2 Rinsing

7
To S L
Procedure
6
To S L
5

FL O W PA N E L
4
Clean >250 mL Container

3
TO
DI Water
0.125"ID 0.250"OD Tubing, 1 Foot
2
9P SI
#2 Phillips Screwdriver
1
12P SI
32004
1. Initialize Analyzer, and remove Left Side Cover.
Figure 5-23 Vacuum/Pressure Manifold 2. Extend Air Supply out of Analyzer (Vacuum/Pressure
Assembly (RR-7)).
8#0#9HULILFDWLRQ#3URFHGXUHV VP-61 Hard Drive Formatting
3. Measure 250 mL (1 cup) of DI water into a clean container.
5P SI 12"H g 8"H g 12P SI 12"H g

7
To S L
&$87,21=##Do not place more than stated amount of
6
DI water in container. To S L
FL O W PA N E L
4
4. Refer to Figure 5-24. Remove tubing from position 5 of
manifold, and attach 0.125"ID 0.250"OD tubing to fitting at
TO
position 5.
5. Place tubing connected to position 5 into container, and
9P SI
allow all DI water to be aspirated into Vacuum
Accumulator #2.
12P SI
6. Remove tubing from position 5, and connect original
32004
tubing.
Figure 5-24 Vacuum/Pressure Manifold
7. Wait at least 5 minutes to remove any saline bridges
between electrodes.
VP-61 Hard Drive Formatting
DIAGNOSTICS Required Tools & Materials:
MORE CELL-DYN 3200 System Service Disk PN 9380700
IDRAIN ACCUMULATORS
9. Initialize Analyzer and repeat Step 8. Verification Procedure
1. Perform (VP-64) Backup Procedure .
2. Turn System OFF.
3. Insert Service Disk into Floppy Drive.
4. Turn System ON.
5. At A:\ prompt type CHKDSK/F, then press
ENTER
8#0#9HULILFDWLRQ#3URFHGXUHV VP-62 DOS Installation
6. If there is any indication that the disk is defective, it should 3. Turn System ON.
be formatted.
4. At A:\ prompt type INSTALL, then press
7. Format the hard drive: ENTER
a. Type FORMAT C:/S and press
5. Remove Service Disk.
ENTER
6. Turn System OFF, wait 5 seconds, then turn System ON.
b. The display will indicate when the format is complete
8. Perform (VP-65) Restore Setup VP-63 CS/SL SETUP
.
1 ,2 7 9 ,6 8 8 ,7 0 4 b y te s to ta l d is k sp a c e
1 3 1,0 7 2 b y te s in 2 h id d e n file s Required Tools & Materials:
3 ,1 7 8 ,4 9 6 b yte s in 9 7 d ire cto rie s
None
7 5 0 ,22 3 ,3 6 0 b y te s in 2 ,2 1 5 u s e r file s
5 2 6 ,1 5 5,7 7 6 b y te s a v a ila b le o n d is k Verification Procedure
3 2 , 7 6 8 b y te s in e a c h a llo c a tio n u n it 1. Enter Diagnostics Hidden Screen
3 8 ,0 53 to ta l a llo ca tio n u n its o n d is k
a. From MAIN MENU, press
16 ,0 5 7 a v a ila b le a llo ca tio n u n its o n d is k
DIAGNOSTICS
6 5 5 ,3 6 0 to ta l b yte s m e m ory
3 2 0 95
b. Press
6 1 8 ,5 6 0 b yte s fre e
F12
Figure 5-25 CHKDSK/F Results, Hard Disk F1
TOGGLE CS/SL to appropriate instrument type.
VP-62 DOS Installation NOTE: Instrument type will display on screen.
c. Press
RETURN
Service Disk PN 9380700
1. Turn System OFF.
2. Insert Service Disk into Floppy Drive.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-64 Backup Procedure
VP-64 Backup Procedure 4. At A:\ prompt, type R to RESTORE, then press

ENTER
Required Tools & Materials: This copies Old Version Setup from Setup Disk to Hard
CELL-DYN 3200 System Setup Disk PN 9380595 Drive.
5. Remove Setup Disk.
6. Turn System OFF, wait 5 seconds, then turn System ON.
1. Turn System OFF.
2. Insert Setup Disk. VP-66 List Mode Data Collection
3. Turn System ON.
4. At A:\ prompt, type S to SAVE, then press
None
ENTER.
This copies Old Version Setup from Hard Drive to Setup Verification Procedure
Disk.
1. Collect Data with List Mode ON.
5. Remove Setup Disk.
a. From MAIN MENU, Press
6. Turn System OFF, wait 5 seconds, then turn System ON. DIAGNOSTICS
b. Press,
VP-65 Restore Setup F12
F1
Required Tools & Materials: STORE RAW ON/OFF (To ON)
CELL-DYN 3200 System Setup Disk PN 9380595 c. Press,
RETURN
Verification Procedure d. From MAIN Menu, press
1. Turn System OFF. RUN
2. Insert Setup Disk. e. Run specimens to collect list mode data.
3. Turn System ON.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-67 Download HSSL Com
f. Press Example: COPY C:\RAWFILE\960921\8411.RAW B:.

MAIN If multiple files need to be downloaded, use wild cards.
DIAGNOSTICS Such as sequence # 841?.raw. This will download
8410, 8411, 8412, 8413, etc.
g. Press,
F12 3. If Needed - Delete Data Files from Hard Drive.
F1 a. At C:\ prompt type CD\RAWFILE, and press
h. DO NOT run analyzer with List Mode ON for an ENTER
extended period. Each file = approximately 100kb. b. Type DIR, and Press
When Hard Drive is filled with Data Lists Fatal Fault
ENTER
condition will result. Then files must be deleted.
to get listing of all files in RAWFILE directory.
2. Download List Mode Data to Diskette.
c. Select files to be deleted.
a. From MAIN MENU, press
d. Type DEL C:\RAWFILE\(FILE NAME) for each file to
F12 be deleted.
F8
4. Press
b. Type CD\RAWFILE\(yymmdd-the DATE OF
COLLECTION). RETURN
c. Press
ENTER
VP-67 Download HSSL Com
NOTE: Date format is YYMMDD (Year Month Day). Required Tools & Materials
Example: 960321 = 21 March 1996.
CELL-DYN 3200 System Installation Disks 1 & 2 P.N. 9380590
d. Place Formatted 1.44mb diskette in Floppy Disk Drive.
e. Type Verification Procedure
COPY C:\RAWFILE\960321\(SEQUENCE#).RAW B:.
This will copy one file of LIST MODE DATA from Data 1. Turn system power OFF.
Station to Diskette in Floppy Drive. 2. Insert disk #1 in the floppy drive.
NOTE: Sequence # = Specimen Sequence number in 3. Turn system power ON.
Data Log.
4. When instructed, insert disk #2.
8#0#9HULILFDWLRQ#3URFHGXUHV VP-67 Download HSSL Com
5. Follow on-screen instructions to download HSSL Com and

setup CS or SL.
a. Go to the Main Menu and press F12, F8 to get to the
C:\CD3200> prompt.
b. Type HSSL
c. If analyzer is an SL, type 0KL <enter>. SKL should be
displayed in the left column. Type 0K <enter>. 0K
should be displayed in the right column.
d. If analyzer is a CS, type 0KS <enter>. 0KS should be
displayed in the left column. Type 0K <enter>. 0K
should be displayed in the right column.
NOTE: 0 = zero
a. Press F8 to exit to the C:\CD3200> prompt.
b. Turn off analyzer, wait 5 sec, turn on analyzer to
reboot.

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CD 3200 Service Manual PDF

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1 - Introduction

CELL-DYN 3200 SYSTEM

1.2 Revision Log

Table 1-1 Revision Log

Revision Date Pages Revised/Added TSBs Incorporated ISAs Incorporated

1.3 About This Manual Section 4: Removal & Replacement

Section 3: Parts Lists

1.5 Warranty Information System External Components

System Internal Components Signal Words and Graphic Warning

Clean-up spills of potentially infectious materials in accordance

Electrical Safety The Class 1 Laser Product Label (Abbott PN 9230702A) is

DANGER: LASER LIGHT Avoid direct exposure to

&$87,21: Use of controls or adjustments or

Figure 1-2 Laser Warning Label (PN9230702D)

1.8 CELL-DYN 3200 Hematology

Table 1-2 Red Cell Parameters

Full Name Abbreviation

Table 1-3 White Cell Parameters

Table 1-3 White Cell Parameters (Continued) 1.9 System Specifications

Table 1-7 Analyzer Input Requirements Table 1-8 Performance Specifications

Table 1-9 Operational Specifications

CS Model Bar Code Specifications

Open 80 Samples/Hour Printed on good quality label stock

2.1 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2 IP-25 Hard Drive Errors . . . . . . . . . . . . . . . . . . . . . . . . . 2-78

2.1 Summary c. To refer to the appropriate IP or VP, click on the red

2.2 Troubleshooting Approach

2.3 Error Messages Error Corrective Action

Incomplete Aspiration IP-3 Shear Valve Position Fault IP-20

Error Corrective Action

IP-1 Fluid Leaks, Open Mode Probe or Wash Block (Page 1 of 2)

- Clamp tubing at the top of

- Verify pinch tubing in

IP-1 Fluid Leaks, Open Mode Probe or Wash Block (Page 2 of 2)

- Verify pinch tubing in - Run a several Count

IP-2 Fluid Leaks, Closed Mode Needle or Wash Block (Page 1 of 2)

- Clamp tubing at the top of

IP-2 Fluid Leaks, Closed Mode Needle or Wash Block (Page 2 of 2)

- Verify pinch tubing in - Run a several Count

IP-3 Incomplete Aspiration (Page 1 of 3)

- Verify Y-Valve is rotating - Aspirate Normal

YES - Replace Y-Valve Assy (PL-8) .

IP-3 Incomplete Aspiration (Page 2 of 3)

- Manually place a blood

- Clean Closed Probe (VP-51).

IP-3 Incomplete Aspiration (Page 3 of 3)

- Run an open cycle and

- Verify Vac 2 level (VP-16). Troubleshoot as necessary.

IP-4 Flow Errors (Page 1 of 2)

From Main Menu:

IP-4 Flow Errors (Page 2 of 2)

- Remove and clean Delivery

- Remove, inspect, and clean

- Replace Laser (PL-10).

IP-5 RBC/HGB Diluent Overpressure (Page 1 of 2)

- Verify Shear Valve rotation time

- Replace Sensor (PL-4.2)

IP-5 RBC/HGB Diluent Overpressure (Page 2 of 2)

In Ready Mode, try to manually

- Replace defective section:

IP-6 Optical Parameter Count Problems (Page 1 of 11)

IP-6 Optical Parameter Count Problems (Page 2 of 11)

NOC Only WBC Only

RBC & PLT