Alex Randall

English 2100 Online

March 30, 2017
Jolynne Berrette

Extended Definition
Draft 2:

Fluorescence Spectrometry

Formal Definition:
Fluorescence spectrometry is a type of electromagnetic spectrometry that inspects fluorescence
from a specimen.

Parenthetical Definition:
Using fluorescence spectrometry (a type electromagnetic spectrometry that examines
fluorescence), the lab team was able to get the molecules to emit visible light.

Fluorescence Spectrometry

Fluorescence Spectrometry is used in the chemical and medical field. It is used to determine the
level of a substance in which its chemical constitutes are being measured and established within
a solution that is based on its fluorescent properties, it is a fast, reliable and simple analysis.
Using a beam of light, most of the time an ultraviolet light, it is aimed at the specimen and
causes the electrons in some molecule compounds
to stimulate. In this case, it causes the molecules to
emit light. This process is completed by using a
device called a fluorometer.

In the diagram above the light source is shot through the solution in the sample cuvette, the light
that passes through is then measured. In fluorescence spectrometry, the light that passes through
(excitation) and the light that is emitted by the sample (emission) can both be measured.

Medical Field
Fluorescence spectrometry is not only used by chemists its also used for diagnostic purposes in
the medical field. It helps to see different types of microorganisms in bacterias, viruses, fungi,
etc. There are a lot of different types of diagnostic methods and techniques but fluorescence
spectrometry seems to be the fastest, most reliable and promising diagnostic technique. It is
mainly used in the microbiology field (obviously).

Diagnostics

Many studies have reported the success in fluorescence spectroscopy as a great diagnostic tool
for bacteria at genus, species and group level by the use of something called spectral
fingerprints. Spectral fingerprints are a(n)
fingerprint for atoms, elements, or
molecules.

Pictured in diagram is a photo of a

fluorescence spectroscopy machine.

References :
http://www.chromedia.org/chromedia?
waxtrapp=mkqjtbEsHonOvmOlIEcCArB&subNav=cczbdbEsHonOvmOlIEcCArBP
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2787503/
https://www.google.com/search?
q=Fluorescence+Spectroscopy&espv=2&source=lnms&tbm=isch&sa=X&ved=0ahUKEwjevp2ws__S
AhUiS2MKHTEaBpYQ_AUIBigB&biw=1280&bih=648#tbm=isch&q=fluorescence+spectroscopy+m
achine&*&imgrc=cmhvsOuoEWrmGM: