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Which of the following would be considered the definitive host of a parasite:

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Humans
Insect vector
First host infected by the parasite
Host in which mature parasites develop

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A parasite's definitive host is defined as the host in which sexual reproduction occurs.

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This suspicious form, found in a stool sample, measures 15 micro meters.

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Entamoeba histolytica
Entamoeba hartmanni
Entamoeba coli
Pseudoparasite

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Entamoeba hartmanni and Entamoeba histolytica are basically identical with the
exception of size. Entamoeba hartmanni is typically smaller that Entamoeba
histolytica measuring less than 10 micro meters.

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This suspicious form was recovered in blood. Please identify the species listed below
that matches the parasites shown in the image with arrows.

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Plasmodium vivax
Plasmodium malariae
Plasmodium falciparum
Pseudoparasite

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Plasmodium falciparum ring forms often resemble a walkman headset by containing


two chromatin dots instead of one, as commonly seen in the other Plasmodium
species. These dots serve as connecting points for the ring of cytoplasm.

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Which one of the following parasites migrates through the circulation (blood) before
maturing in the portal venous system?

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Schistosoma mansoni
Enterobius vermicularis
Taenia saginata
Trichuris trichiura
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Schistosoma mansoni is a fluke that resides in the portal system venules, primarily
those of the lower intestine. Cercariae (the free-swimming larval stage of the fluke) is
released from infected snails (the intermediate host) into freshwater estuaries.
Humans that are in the water may become infected through skin penetration by the
cercariae. The cercaria then enters the blood circulation, finally settling into the
intrahepatic portal blood vessels of the intestine where it matures.

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Which of the following organisms is an obligate intracellular parasite:

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Proteus
Escherichia
Klebsiella
Rickettsiae

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Rickettsia are gram-negative coccobacilli which are classified as obligate intracellular


parasites. They are able to invade various eukaryotic host cells such as
reticuloendothelial cells and vascular endothelial cells. Rickettsiae are transmitted to
humans through infected arthropods, and hard ticks serve as both vectors and
reservoir hosts for most spotted fever group (SFG) rickettsiae

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I measure 12 micro meters and am found in stool.


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Pneumocystis carinii cyst


Entamoeba coli cyst
Endolimax nana cyst
Iodamoeba butschlii cyst

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The cysts of Iodamoeba butschlii may be readily identified by their size


(approximately 12 micro meters) and by the presence of a well-defined glycogen
mass. Furthermore, the large karyosome and absence of peripheral chromatin that
constitutes the single nucleus help to distinguish them from the other amebic cysts.

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Match the organisms on the right with their appropriate type from the drop down box:

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Protozoa Trypansoma, Leischmania


Helminths Trichinella, Schistosoma
Protozoa Plasmodium
Helminths Ascaris, Cestodes

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Protozoa: Trypansoma, Leischmania, Plasmodium

Helminths: Trichinella, Schistosoma, Ascaris, Cestodes

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Multiple ring forms are most frequently seen in which Plasmodium?

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falciparum
vivax
malariae
ovale
barundi

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Multiple ring forms are most frequently seen in Plasmodium falciparum. The
trophozoite develops into a schizont made up of multiple ring forms (also known as
merozoites) via schizogony, or asexual replication. The schizont then matures which
causes the rupture of the erythrocytes- releasing merozoites into the circulation which
in turn infect other erythrocytes

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Adult Ascaris lumbricoides worms live in the:

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Lungs
Liver
Small Intestines
Large Intestines

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Although the larval form of Ascaris migrates through the lung, where it can cause
Loeffler's syndrome, the mature form lives in the duodenum or proximal jejunum. The
adults are very large worms, and can measure up to 35 cm in length.

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This parasite resides in human intestine.

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Taenia solium scolex


Hymenolepis nana scolex
Dipylidium caninum scolex
Diphyllobothrium latum scolex

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The Taenia solium scolex is identified by the presence of four suction-cuplike suckers
and the presence of a fleshy rostellum with hooklets.

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Match the organism names from the drop down box to the name of the associated
condition listed below.

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Plasmodium ovale Malaria


Trypanosoma cruzi Chagas' Disease
Cestodes Tapeworms
Trypanosoma rhodesiense Sleeping Sickness

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Plasmodium ovale: Malaria

Trypanosoma cruzi: Chagas' Disease

Trypanosoma rhodesiense: Sleeping Sickness

Cestodes: Tapeworms

Question Difficulty: Level 5

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The iodine prep is least helpful in the identification of which of the following parasitic
stages:

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Larvae
Eggs
Cysts
Trophozoites

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Trophozoites are not generally motile in iodine stained wet preps. Typically, a small
drop of iodine stain may be placed on the slide prior to adding the coverslip. The slide
is then thoroughly examined microscopically for larvae, eggs, and cysts.

Question Difficulty: Level 5

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I measure 15 micro meters and am found in stool.

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Entamoeba histolytica cyst


Entamoeba hartmanni cyst
Entamoeba coli cyst
Endolimax nana cyst

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Entamoeba histolytica cysts are characterized by their size (usually larger than 10
micro meters) and the presence of 2 to 4 nuclei each consisting of a small central
karyosome inside a ring of evenly distributed peripheral chromatin. Thin chromatin
bars are also common in older cysts.

Question Difficulty: Level 7

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The trophozoite state of the intestinal flagellate Giardia lamblia is characterized by:

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two nuclei
an undulating membrane
four flagella
pattern of the karyosome
spoked-wheel nucleoli

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The trophozoite state of the intestinal flagellate Giardia lamblia is characterized by


two nuclei. It also has a large sucking disk which it uses to attach to a host's intestinal
wall lining. The trophozoite is usually shaped like a pear and utilizes four pairs of
flagella for propulsion.

Question Difficulty: Level 6

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What is the anatomical feature of a tapeworm that possesses both male and female
reproductive structures?

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Brood capsule
Proglottid
Rostellum
Scolex

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The body of a tapeworm is composed of successive segments known as proglottids.


Each mature proglottid has both male and female reproductive structures.

Question Difficulty: Level 6

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This suspicious form, shown below at both low (10X) and high dry (40X) power,
measures 90 micro meters by 42 micro meters. It was seen in a stool sample.

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Hookworm egg
Pseudoparasite
Ascaris lumbricoides egg
Diphyllobothrium latum egg

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The mature eggs of Ascaris lumbricoides are readily visible at both 10X and 40X dry.
The organism size as well as the presence of an outer albuminous coating help to
identify the egg.
Question Difficulty: Level 4

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Which of the following has a life cycle that most closely resembles that of
Plasmodium sp:

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Dracunculus medinensis
Blastocystis hominis
Babesia microti
Toxocara canis

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Like malaria, Babesia is a protozoan which infects red cells and produces a febrile
illness. Unlike malaria, which is spread by mosquitoes, Babesia is transmitted by
ticks.

Question Difficulty: Level 4

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