MANUAL

3025
FLUORESCENCE MICROSCOPE
SYSTEM

73 Mall Drive, Commack, NY 11725 631-864-1000 (P) 631-543-8900 (F)

www.accu-scope.com info@accu-scope.com
 3025 FLUORESCENCE MICROSCOPE SYSTEM

CONTENTS
SAFETY NOTES .................................................................................................................. 3

CARE AND MAINTENANCE ................................................................................................ 3

INTRODUCTION .................................................................................................................. 4

UNPACKING AND COMPONENTS ..................................................................................... 4

EPI FLUORESCENCE ATTACHMENT DIAGRAM .............................................................. 4

EPI FLUORESCENCE ATTACHMENT ASSEMBLY ............................................................ 5

DETAILED ASSEMBLY PROCEDURE ................................................................................ 6

ADJUSMENT & OPERATION

COMPONENTS DIAGRAM................................................................................................. 8
ADJUSTING THE ROTATION TENSION
OF THE FLUORESCENCE MIRROR BLOCK (FILTER BLOCK) ..................................... 9
PREPARATION ............................................................................................................... 10
CENTERING THE FIELD IRIS DIAPHRAGM ..................................................................... 11
ADJUSTING THE FIELD IRIS DIAPHRAGM ...................................................................... 11
CENTERING THE APERTURE IRIS DIAPHRAGM ............................................................. 12
ADJUSTING THE APERTURE IRIS DIAPHRAGM ............................................................. 12
CENTERING THE MERCURY BURNER ........................................................................... 13
CENTERING THE MIRROR REFLECTED IMAGE ............................................................. 14
MOUNTING ND FILTER................................................................................................... 14

TROUBLESHOOTING........................................................................................................ 15

MAINTENANCE.................................................................................................................. 16

SERVICE ............................................................................................................................ 16

WARRANTY ....................................................................................................................... 16

ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 631-864-1000 www.accu-scope.com 2

 3025 FLUORESCENCE MICROSCOPE SYSTEM

SAFETY NOTES
1. Open the shipping carton carefully to prevent any accessory, i.e. objectives or eyepieces, from
dropping and being damaged.
2. Do not discard the molded Styrofoam container; the container should be retained should the
microscope ever require reshipment.
3. Keep the instrument out of direct sunlight, high temperature or humidity, and dusty environments.
Ensure the microscope is located on a smooth, level and firm surface.
4. If any specimen solutions or other liquids splash onto the stage, objective or any other component,
disconnect the power cord immediately and wipe up the spillage. Otherwise, the instrument may be
damaged.
5. CAUTION: the lamp, lamp housing and adjacent parts will become very hot. Do not touch these parts
until they have completely cooled. Never attempt to handle a hot halogen bulb.
6. All electrical connectors (power cord) should be inserted into an electrical surge suppressor to
prevent damage due to voltage fluctuations.
7. For safety when replacing the halogen lamp or fuse, be sure the main switch is off (O), remove the
power cord, and replace the halogen bulb after the bulb and the lamp house has completely cooled.
8. Confirm that the input voltage indicated on your microscope corresponds to your line voltage. The
use of a different input voltage other than indicated will cause severe damage to the microscope.

CARE AND MAINTENANCE

1. Do not attempt to disassemble any component including eyepieces, objectives or focusing assembly.
2. Keep the instrument clean; remove dirt and debris regularly. Accumulated dirt on metal surfaces
should be cleaned with a damp cloth. More persistent dirt should be removed using a mild soap
solution. Do not use organic solvents for cleansing.
3. The outer surface of the optics should be inspected and cleaned periodically using an air stream from
an air bulb. If dirt remains on the optical surface, use a soft cloth or cotton swab dampened with a
lens cleaning solution (available at camera stores). All optical lenses should be swabbed using a
circular motion. A small amount of absorbent cotton wound on the end of a tapered stick such as
cotton swabs or Q-tips, makes a useful tool for cleaning recessed optical surfaces. Avoid using an
excessive amount of solvents as this may cause problems with optical coatings or cemented optics
or the flowing solvent may pick up grease making cleaning more difficult. Oil immersion objectives
should be cleaned immediately after use by removing the oil with lens tissue or a clean, soft cloth.

4. Store the instrument in a cool, dry environment. Cover the microscope with the dust cover when not
in use.
5. ACCU-SCOPE microscopes are precision instruments which require periodic preventative
maintenance to maintain proper performance and to compensate for normal wear. An annual
schedule of preventative maintenance by qualified personnel is highly recommended. Your
authorized ACCU-SCOPE distributor can arrange for this service.

ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 631-864-1000 www.accu-scope.com 3

 3025 FLUORESCENCE MICROSCOPE SYSTEM

INTRODUCTION
Congratulations on the purchase of your new ACCU-SCOPE fluorescent microscope. ACCU-SCOPE
microscopes are engineered and manufactured to the highest quality standards. Your microscope will
last a lifetime if used and maintained properly. ACCU-SCOPE microscopes are carefully assembled,
inspected and tested by our staff of trained technicians in our New York facility. Careful quality control
procedures ensure each microscope is of the highest quality prior to shipment.

UNPACKING AND COMPONENTS

Your microscope arrived packed in a molded Styrofoam container. Do not discard the container: the
Styrofoam container should be retained for reshipment of your microscope if needed. Avoid placing the
microscope in dusty surroundings or in high temperature or humid areas as mold and mildew will form.
Carefully remove the microscope from the Styrofoam container by its arm and base and place the
microscope on a flat, vibration-free surface.

EPI FLUORESCENCE ATTACHMENT DIAGRAM

Main body of the EPI Fluorescence Attachment

Power Supply Unit

Power Cord (ONLY use the power cord provided)

A Mercury Lamp

Fuses (8A)

ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 631-864-1000 www.accu-scope.com 4

 3025 FLUORESCENCE MICROSCOPE SYSTEM

EPI FLUORESCENCE ATTACHMENT ASSEMBLY

The diagram below shows how to assemble the various modules. The numbers indicate the order of
assembly.

When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching
any parts or touching glass surfaces.

Loosen the set screw and remove the trinocular viewing head from the body of the microscope.

Insert the EPI Fluorescence attachment into the microscope and tighten the set screw until it is firmly
in place.

Insert the trinocular viewing head into the EPI Fluorescence attachment as shown and tighten the set
screw until it is firmly in place.

ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 631-864-1000 www.accu-scope.com 5

 3025 FLUORESCENCE MICROSCOPE SYSTEM

ASSEMBLY (continued)
Mounting the Mercury Burner
(Fig.1 & Fig. 2)

Loosen the lamp housing (burner socket)

clamping screw , and remove the housing
cover.Fig.1

Remove the foam backstop and insert lamp

by using a soft tissue into the (positive) + pole
(the wide head) of the mercury burner to the
lower terminal first and then the (negative)
pole (the thin head) to the upper terminal, then
Fig. 1 tighten the two socket clamping screws .

Slide the lamp housing cover back on, and

tighten the housing clamping screw .

Be sure to ONLY use a 100 watt mercury

lamp.
Be sure to mount the positive + pole (the
wide head) before the negative pole, or
damage to the lamp may occur.
Never use excessive force when mounting
the mercury lamp.

Avoid touching or soiling the mercury lamp. If
this happens, gently wipe it with lens tissue or
a soft, clean cloth.
Fig. 2 To prevent hazard, always turn the main
switch on the power supply unit to O (OFF)
position, unplug the power cord plug from the
main outlet, and wait for a minimum of 10
minutes before replacing the lamp.

Mounting Filter Blocks (Fig.3 and Fig.4)

Unscrew the Allen screws with the
included Allen wrench and take out the filter
block turret .

Allen
screw

Fig. 3

ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 631-864-1000 www.accu-scope.com 6

 3025 FLUORESCENCE MICROSCOPE SYSTEM

Invert the filter block turret , (several model

blocks can be found.) Loosen the Allen screw
to take one of the blocks out.
Mount a filter cube (Fig. 3) into the top of the
model block and tighten the screw . Beside the
screw, you can see a number on the turret
indicating G-excitation. To help identify mirror
blocks, place a label below the corresponding
number on the front side of the mirror turret.
Repeat procedure to mount other blocks.

Fig. 4 Push the filter block turret back into the rail slot
and tighten the Allen screw.

Mounting Protection Barrier (Fig.5)

Install the protection barrier on the attachment by
tightening the screws.

Assembly of the Fluorescent Attachment

(Fig.6)
Fig. 5 Mount the lamp housing into the other end of
the attachment and secure it with two screws .

Cable & Cord Connections

(Fig. 7 & Fig. 8)
Make sure that the main switch of the power
supply is set to O (OFF) position before
connecting cables.
Plug the connector from the lamp socket
securely into the connector on the power supply
Fig. 6 unit.
Plug the power cord connector into the power
supply unit.
Verify that the voltage and the frequency of the
AC main outlet match the setting of the voltage
switch and the frequency switch on the rear of the
power supply unit as improper setting may result in
poor lamp performance, or in rare instances cause
the lamp to explode.
Only use the power cord provided by the
manufacturer. Should you lose or damage the
power cord, contact the manufacturer or your
Fig. 7 distributor.

ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 631-864-1000 www.accu-scope.com 7

 3025 FLUORESCENCE MICROSCOPE SYSTEM

Fuse Replacement (Fig.7 & Fig. 8)

Set the main switch to O (OFF) position and
unplug the power cord before replacing fuses.

Using a flat-head screwdriver to loosen and

remove each of the fuse holders .

Replace both fuses with new ones.

Fig. 8
Always use the designated fuse (8A). And
make sure the voltage of the fuse matches
the voltage of the AC main outlet.

ADJUSTMENT & OPERATION

Components Diagram

Fig. 9

ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 631-864-1000 www.accu-scope.com 8

 3025 FLUORESCENCE MICROSCOPE SYSTEM

Fig. 10

Adjusting the Rotation Tension of the

Fluorescent Mirror Block (Filter Block)
There are 6 fluorescent mirror blocks (filter
blocks) mounted in the filter block turret.
(Fig.11).

A mirror block includes a dichrohic mirror, a

barrier filter, and on excitation filter. Do not take
apart the filter block.

A wide variety of filter sets are available. Please

consult with your ACCU-SCOPE distributor to
select the appropriate set for your application.
Fig. 11

ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 631-864-1000 www.accu-scope.com 9

 3025 FLUORESCENCE MICROSCOPE SYSTEM
OPERATION
Preparation
Verify that the voltage and the frequency of the AC
main outlet match the setting of the voltage switch
and the frequency switch on the rear of the power
supply unit.
Make sure the cord is connected firmly.
When transmitted light observation is required, pull
out the filter system and rotate the blank opening
into the light path.
Adjust the field diaphragm to match the field edge.
If it is not centered, use the Allen wrench to adjust
the screw.
When it is required to interrupt observation for a
short period, use the shutter mechanism on the
filter wheel to block the light accessorial excitation
filter part. (Repeated on-off of the mercury lamp
will shorten its service life considerably).
Precautions on the specimen color fading:
ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 631-864-1000 www.accu-scope.com
The system employs high-intensity excitation light
to enable bright observation of dark fluorescence
specimens. As a result, if high-power objectives
are used frequently, color fading of the specimen
occurs early, degrading the view (contrast) of
fluorescence images. So it is effective to use the
shutter frequently to avoid illuminating the
specimen for a longer period than required.
A ND (neutral density) filter and small aperture
diaphragm can help weaken the intensity of the
excitation light. Also, it is useful to use the light
shutter to reduce the specimen color fading.
Color fading of the specimen can also be delayed
using commercially available color fading
preventing agent (DABCO, etc). The use of color
fading preventing agent is recommended when
you perform high-magnification observation
frequently.
NOTE: color fading preventing agent cannot be
used with certain specimens
10
 3025 FLUORESCENCE MICROSCOPE SYSTEM

Centering the Field Iris Diaphragm (Fig.1)

Switch the light shutter to closed position.

Turn filter block turret to engage the B-excitation

mirror in the light path.

Switch the light shutter to O open position.

Rotate the 10 objective in the light path, and
place the specimen on the stage and bring into
approximate focus.
Fig. 1
Pull the field iris diaphragm lever out until
the diaphragm comes into the smallest state.

Use the Allen wrench to adjust the two field

iris diaphragm centering screws alternately
to move the image of the diaphragm to the
center. (Fig. 2 shows the adjustment of
diaphragm)

Move the field diaphragm lever to open the

diaphragm. As this makes slight deviation
noticeable, adjust the centering precisely.

Enlarge the diaphragm until it just

circumscribes the field of view.

Adjusting the Field Iris Diaphragm

(Fig. 2)
The field diaphragm adjusts the diameter of the
illuminating beam to obtain good image contrast.

Keeping the field diaphragm stopped down to

the smallest required area for each observation
makes it possible to prevent color fading of
areas outside the observation target region.

According to the objective in use, adjust the

Fig. 2 diaphragm image using the field diaphragm
lever so that the field of view is circumscribed by
the field diaphragm to exclude stray light.

ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 631-864-1000 www.accu-scope.com 11

 3025 FLUORESCENCE MICROSCOPE SYSTEM

Centering the Aperture Iris Diaphragm

(Fig. 3)
Switch the light shutter to close position to
turn off the light path.
Rotate the filter block turret to engage the G-
excitation mirror block or another into the light
path.
Fig. 3 Switch the light shutter toO open position
to open the light path.
Rotate the 10 objective in the light path, and
place the centering plate (a white plate with a
cross) on the stage and bring into approximate
focus.
Move the cross of the centering plate to the
center of the field of view.

Pull out the aperture diaphragm lever to

adjust the aperture iris diaphragm to the
smallest diameter.

Pull out the field iris diaphragm lever to adjust

the field iris diaphragm to the smallest diameter.
The image of aperture iris diaphragm can be
found on the centering plate.
Adjust the aperture iris diaphragm centering
screws with attached wrench to superpose
the image of aperture iris diaphragm on the
cross of centering plate.

Adjusting the Aperture Iris Diaphragm (Fig. 3)

The aperture iris diaphragm adjusts image resolution and contrast.

For fluorescence observation, push in the aperture iris diaphragm lever .

Both ND (neutral density) filter and small aperture diaphragm can help weaken the intensity of the
excitation light to delay color fading of the specimen

According to the objective in use, adjust the diaphragm image using the field iris diaphragm lever so
that the field of view is circumscribed by the field iris diaphragm to exclude stray light.

ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 631-864-1000 www.accu-scope.com 12

 3025 FLUORESCENCE MICROSCOPE SYSTEM

Centering the Mercury Lamp (Fig. 4-6)

NOTE: Before proceeding to center the lamp, wait
for the arc image to stabilize to protect against
glare during arc image centering, it should be
viewed across the excitation light protective shield.

Switch the light shutter to close position to

shut off the light path.

Rotate the filter block turret to engage the green or

blue excitation filter block into the light path. If U/V
Fig. 4 excitation filter block is used, be sure to use the
protective shield.

Revolve the nosepiece to engage 10 objective

into the light path. Place the centering plate on
stage, through transmission observation; adjust the
stage until the cross is in the centre of the field of
view.

Remove the objective from the revolving

nosepiece position and engage this position in the
light path.

Pull out the field iris diaphragm lever to close

the iris diaphragm and push in the aperture iris
diaphragm lever to open the iris diaphragm to
the large limit.

Switch the light shutter to O open position to

open the light path.

Turn the collector adjusting knob to project the

arc image on the centering plate and sharpen it.
(A)

Turn the lamp adjusting knob to move the arc

image and the mirror reflected arc image in the
symmetrical position. (B)

Adjust the mirror focusing knob (Fig.6) to

sharpen the mirror reflected arc image. (C)

Turn the burner adjusting knob to overlap the

arc image with the mirror reflected arc image.(D)

Fig. 5 Turn the collector adjusting knob to make the

field of view as bright as regular as possible..

Maintain this condition until the next time the lamp

is replaced.

ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 631-864-1000 www.accu-scope.com 13

 3025 FLUORESCENCE MICROSCOPE SYSTEM

Centering the Mirror Reflected Image

(Fig. 6)
The mirror reflected image has been centered at
the factory. Do not adjust the knob -- only if
necessary. Only when the burner has been
centered precisely, can the knob be adjusted.

NOTE: Once the knob is adjusted, the reflected

mirror cannot be reconverted to the factory
setting.

Knob Control (Fig. 6
Fig. 6 The middle knob is the mirror reflected image
focusing knob which can focus the reflected
image.

The knobs on both sides can adjust the

up/down or left/right position of the mirror
reflected image.

Mounting the ND (Neutral Density) Filter

(Fig. 7)
The ND filter can reduce the excitation light
intensity to delay color fading of the specimen.
Use the ND filter it if does not hinder
observation.

There are two kinds of ND filters available: ND6

and ND25 for position and respectively
Fig. 7 (Fig. 7). To prevent the ND filter from being
damaged, insert the filter with the indication
surface facing the observation side.

When the filter is inserted, you will hear two

clicks. The filter is in the light path on the second
click.

CAUTION
When the mercury lamp is lit for a long
period while an ND filter is inserted, the filter
and its metallic frame may become very hot.
Wait until the ND filter cools down before
replacing it.

ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 631-864-1000 www.accu-scope.com 14


TROUBLESHOOTING
Under certain conditions, performance of this unit may be adversely affected by factors other than defects. If a
problem occurs, please review the following list and take remedial action as needed. If you cannot solve the
problem after checking the entire list, please contact your local dealer for assistance.
Optics
PROBLEM
Although the mercury lamp
illumination is on, the field of
view is invisible or dark.
Visibility is poor. Image is
not sharp. Contrast is poor.
The edge of the field of view
is obscured or not evenly
illuminated
Shadow exists in the field of
view
Electrical System
The main switch cannot
supply power to the system
The main switch can be set
to ON but the burner doesnt
ignite
The mercury lamp flickers or
the brightness is low
ACCU-SCOPE
3025 FLUORESCENCE MICROSCOPE SYSTEM
CAUSE
The light shutter closes the light path
The ND filter is engaged in the light path.
The fluorescence mirror block is
improperly engaged in the light path
The aperture iris diaphragm and field iris
diaphragm are not open enough
The objective or filter is dirty
The aperture iris diaphragm and field iris
diaphragm are adjusted improperly
The fluorescence mirror block is not
proper for the specimen
The objective is improperly engaged in
the light path
The fluorescence mirror block is
improperly engaged in the light path
The field of view doesnt open fully
ND filter is stopped in halfway in the light
path
The mercury lamp is not centered.
The collector focus position is not correct
The burner or collector is dusty or stained
The power cord is connected improperly
A fuse is blown
The lamp housing connecting cord is
connected improperly
The mercury lamp is not mounted
The auto ignition system is
malfunctioning
The phenomenon is observed in a short
period after ignition
The lamp life has expired
73 Mall Drive, Commack, NY 11725 631-864-1000 www.accu-scope.com
SOLUTION
Switch the light shutter to O open
position
Pull out ND filter to open the position
Engage it properly
Open the aperture iris diaphragm fully;
adjust the field iris diaphragm to
circumscribes the field of view
Clean them thoroughly
Open these iris diaphragms fully
Use proper mirror block
Make sure the nosepiece clicks properly
into place
Engage it properly in the light path
Open it fully
Pull in the filter slider until it clicks into
place
Center it
Adjust it to an optimum position
Clean them thoroughly
Connect it properly
Replace the fuses
Connect it properly to the connectors
Attach a mercury lamp
Set the main switch of the power
supply unit to OFF then on again.
(Repeated ON-OFF is possible in this
case)
Wait for 10 minutes or more after ignition
Replace the mercury lamp
15
 3025 FLUORESCENCE MICROSCOPE SYSTEM

MAINTENANCE
Please remember to never leave the microscope with any of the objectives or eyepieces removed and
always protect the microscope with the dust cover when not in use.

SERVICE
ACCU-SCOPE microscopes are precision instruments which require periodic servicing to keep them
performing properly and to compensate for normal wear. A regular schedule of preventative maintenance
by qualified personnel is highly recommended. Your authorized ACCU-SCOPE distributor can arrange
for this service. Should unexpected problems be experienced with your instrument, proceed as follows:

1. Contact the ACCU-SCOPE distributor from whom you purchased the microscope. Some
problems can be resolved simply over the telephone.

2. If it is determined that the microscope should be returned to your ACCU-SCOPE distributor or to

ACCU-SCOPE for warranty repair, pack the instrument in its original Styrofoam shipping carton. If you
no longer have this carton, pack the microscope in a crush-resistant carton with a minimum of three
inches of a shock absorbing material surrounding it to prevent in-transit damage. The microscope should
be wrapped in a plastic bag to prevent Styrofoam dust from damaging the microscope. Always ship the
microscope in an upright position; NEVER SHIP A MICROSCOPE ON ITS SIDE. The microscope or
component should be shipped prepaid and insured.

LIMITED MICROSCOPE WARRANTY

This microscope is warranted to be free from defects in material and workmanship for a period of five years from
the date of invoice to the original (end user) purchaser. The mercury power supply is warranted for a period of one
year from the date of invoice to the original (end user) purchaser. This warranty does not cover damage caused in-
transit, misuse, neglect, abuse or damage resulting from improper servicing or modification by other then ACCU-
SCOPE approved service personnel. This warranty does not cover any routine maintenance work or any other
work, which is reasonably expected to be performed by the purchaser. Normal wear is excluded from this warranty.
No responsibility is assumed for unsatisfactory operating performance due to environmental conditions such as
humidity, dust, corrosive chemicals, deposition of oil or other foreign matter, spillage or other conditions beyond the
control of ACCU-SCOPE INC. This warranty expressly excludes any liability by ACCU-SCOPE INC. for
consequential loss or damage on any grounds, such as (but not limited to) the non-availability to the End User of
the product(s) under warranty or the need to repair work processes. Should any defect in material, workmanship or
electronic component occur under this warranty contact your ACCU-SCOPE distributor or ACCU-SCOPE at (631)
864-1000. This warranty is limited to the continental United States of America. All items returned for warranty
repair must be sent freight prepaid and insured to ACCU-SCOPE INC., 73 Mall Drive, Commack, NY 11725
USA. All warranty repairs will be returned freight prepaid to any destination within the continental United States of
America, for all foreign warranty repairs return freight charges are the responsibility of the individual/company who
returned the merchandise for repair.

ACCU-SCOPE is a registered trademark of ACCU-SCOPE INC., Commack, NY 11725

ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 631-864-1000 www.accu-scope.com 16