Phytochemical screening (primary metabolites)

1. Carbohydrates

Molischs test Alpha-naphthol

Ethanol
Concentrated H2SO4

2. Proteins

Millons test Millons reagent

3. Fats and oils

Oil test 1% osmic acid

Solubility test Chloroform

Ethanol

4. Steroid

Salkowski test Chloroform

Concentrated H2SO4

Liebermann- Burchard test Chloroform

Acetic anhydride
Concentrated H2SO4

5. Flavonoids

Shinoda test 95% ethanol/ t-butyl alcohol

Concentrated HCl
Magnesium turnings

Sulfuric acid test Sulphuric acid

 6. Alkaloids

Dragendorffs test Dragendroffs reagent

Mayers test Mayers reagent

Tannic acid test Tannic acid solution

7. Tannins and phenolic compounds

5% FeCl3 solution
Acetic acid solution
Potassium dichromate reagent