CONFIDENTIAL Dated : 09.12.2015 ‘The Head of the Department (HOD), Department of Forensic Medicine & Toxicology, AIIMS, New Delhi Subject :- Opinion in case FIR No 04/15 dated 01.01.15 w/s 302 IPC P.S Sarojini Nagar, New Delhi(SunandaPushkar death case vide PM No 77/14 dated 18.01.14) Ref: Letter No 2562 (2 pages) dated 16.11.15 P.S Sarojini Nagar, New Delhi. Sir Kindly refer to your office letter no CL/FM-18/2014 dated 21.11.2015 on the subject cited as above, it is submitted that exhibits including viscera (stomach,intestine,kidney,spleen), blood sample, lower and upper wearing clothes of the deceased and a white color bed sheet, bed cover used by deceased in Hotel The Lecela Palace, New Delhi were, sent to FBI, USA for further examination. Following three reports have been received from FBILUSA after the examination of abovementioned exhibits. 1. FBI Laboratory Toxicology Report on Sunanda Pushkar Tharoor dated September 9, 2015. 2. Résults of radiochemistry Analysis conducted under the auspices of Dr. James F. + Blankenship of the CBRNSU, dated August 5, 2015. 3. Supplemental FBI Laboratory Toxicology Report on Sunanda Pushkar Tharoor, dated November 6, 2015. In the'letter dated 06 November, 2015 of FBI, USA a reference of letter dated September 3, 2015 was mentioned in report which was not received from FBI, USA in MHA. Therefore, a clarification was sought by MHA in this regard from FBI, USA. The requisite clarification was received in MHA on 07.12.2015 and the same was communicated to DCP/ South District on 08.12.2015. Attested copies of all the above mentioned four letters dated (1) September 9, 2015, (2) August 5, 2015, (3) November 6, 2015 and (4) December 07, 2015 are enclosed herewith for your perusal and further necessary action as désired vide your letter dated 21.11.2015 ‘You are requested to provide your opinion on the issues mentioned in my Letter No 2562 (2 pages) dated 16.11.15 and’any other useful information which may be helpful in theinvestigation of this case on the basis of your examination of the enclosed reporis from FB] Laboratory, USA Note:-In the letter dated December 7, 2015 of U.S. Department of Justice, Criminal Division, Mr. Mark Tellitocci, Trial Attorney mentioned, “ Pursuant to Article 7 of the treaty, authorization of the use of this material is limited to the purposes for which assistance has been requested and granted. Should you anticipate the need to use the material for any other purpose (Le, a use not stated in the, request for assistance and/or not approved in the grant of assistance), please consult this office to ascertain whether authorization or approval can be secured.” As such, it cannot be shared with any unauthorized person or authority without prior permission from U.S Department of Justice. Hf you have any question, please do not hesitate to contact me by telephone at # 8750870835 or O11- 26882346. ~ (VKP.S.Yadav) SHO/Sarojini Nagar" vk p.s Babe? SHO Encls:- -SAROJIN| NAGE ‘OUTH DISTRICT, NEV. - FBI Laboratory Toxicology Report on SunandsPushkarTharoor dated September 9,2015. - 5 PP. . Results of radiochemistry Analysis conducted under the auspices of Dr. James F. Blankenship of the CBRNSU, dated August 5, 2015. —3 PP. . Supplemental FBI Laboratory Toxicology Report on. SunandaPushkarTharoor, dated November 6, 2015. —4 PP. Letter dated Dec. 7, 2015 from U.S. Department of Justice, Criminal Division, Office of International Affairs, Washington D.C. -2 PP.LMS (Re. 714) UNCLASSIFIED 72501 Invesigation Parkway FBI Laboratory Quanies, Virgina 22/38 REPORT OF EXAMINATION Te: Suhel Duad ‘Date: September 09, 2015 ALAT ‘New Delhi (Case IDNo: 163A-NL-6093936 Lab No: 2015-00295.2 Communication(s): February 9, 2015 ‘Agency Reference(s) Subjects): Vietin(s): Sunanda Pushkar Disciplines): Chemistry - Toxicology FBI Labontory Evidence Designaton(s): item 1 ‘Clothing from Sunanda Pushkar Item2 Case with contents from back room Item 3 ‘Nine slides containing histological specimens from Sunanda Pushkar tem 4 Sixteen photographs from crime scene hem 5 Digital viceo dise stem 6 Digital video dise tem 7 ‘Bed cover from bedroom in Room No. 345 of Leela Palace Kem 8 Sheet from bedroom in Room No. 345 of Leela Palace Item 9 ‘Tissue and fluid sample from Sunanda Pushkar (Borle #1, storiach contents/small intestine) hem 10 ‘Tissue and fluid sample from Sunanda Pushker (Bottle #2, organs/viscere) tem U1 Postmortem blood sample from Sunanca Pushkar stem 12 ‘Sample of preservative This report contains the results of the toxicology examinations. Page 1 of 5 UNCLASSTFTEDUNCLASSIFIED Results of Examinations: Item J clothing from Sunanda Pushkar Analyte Result Note(s} Ricinine Not detected. 1 Over-the-counter, prescription | Identified (qualitative) 2 and illicit drugs Nicotine Cotinine Alprazolam Lidoesine Hydroxychloroquine Results of Examinations: Item 7 bed cover from bedroom Item 8 sheet from bedroom Analyte Result Note(s) Ricinine Not detected, 1 Over-the-counter, prescription | Identified (qualitative: 2 and illicit drugs: Nicotine Cotinine Alprazolam Lidocaine Notes: 1+ “Analysis was performed using solid phase extraction (SPE) followed by liquid- chromatography Fourier transform tandem mass spectrometry (LC-FTMS/MS) 2 This screen targeted alkaline drugs such as antihistamines, antidepressants, opioid analgesics, methamphetamine and other stimulants. Analysis was performed using SPE followed by gas chromatographyfnass spectrometry (GC/MS) and LC-FTMSMMS. Page? of 5 8 5-00295-2 UNCLASSIFIEDUNCLASSIFIED Results of Examinations: Item 9 tissue and fluid sample from Sunanda Pushkar (Bottle #1, stomach contents/small intestine) tem 10 tissue and fluid sample from Sunanda Pushkar (Bottle #2, organsiviscera) {tem 11 postmortem blood sample from Sunanda Pushkar Analyte A Result Note(s) Cardiac Glycosides Not detected. i ‘Over-the-counter, prescription | Identified (qualitative, Items 9, 10, 11): 23,4 drugs Alprazolam Hydroxychloroquine Notes: ; 1 Analysis was performed using liquid-liquid extraction (LE) followed by Tiquid- chromstography Fourier transform tandem mass spectrometry (LC-FTMS/MS), This analysis targeted digoxin, digitoxin, and oleandrin. The limit of detection for these analytes has not been established for the matrices that were analyzed. . 2 This analysis consisted of two drug sereens and additional confirmatory assays. The fist screen targeted al¥aline drugs such as antihistamines, antidepressants, opioid analgesics, nethamphetamine and other stimulants. Analysis was performed using solid phase extraction (PE) followed by gas chroreatography/mass spectrometry (GC/MS) and liquid hromatography/mass spectrometry (LC/MS). The second screen targeted acidic and neutral drugs such as barbiturates, seizure medications, and some non-steroidal ant-inflemmatory drugs, ‘Analysis was performed using liqui/Iiquid extraction (LLE) followed by GC/MS. 3°” This analysis targeted benzodiazepines and was peiformed by SPE followed by LC- FTMSMS. 4 The Jtem 11 postmorterh blood sample was also analyzed by immunoassay for benzoylecgonine, benzodiazepines, and marijuana metabolites with inconclusive results. Results of Examinations: Item 12 sample of preservative Analyte Result Note(s) Over-the-counter, prescription | Not detected. . 1 and illicit drugs 1. This soreen targeted alkaline drugs such as antihistamines, antidepressants, opioid analgesics, metiamphetamine and other stirmulants. Analysis was performed using SPE followed bby gas chromatography/mass spectrometry. (GC/MS) and LC-FTMS/MS. Page 3 of 5 2015-00295-2 é UNCLASSIFIEDUNCLASSIFIED Limitations: ‘The FBI Laboratory hes performed screening in this case for numerous drugs, some of which are only indicated by a general drug class ebove. Drug dosages, drug metabolism rates, and laboratory detection limits for drugs and metabolites vary. For questions about whether or nota specific drug or drug metabolite would have been detected in this analysis, please contact the examiner issuing this report. ‘The following items were not analyzed by chemical methods: Tem 2 Case with contents from back room Stem 3 Nine slides containing histological specimens from Sunanda Pushkar (The FBI Laboratory does not currently conduct histology examinations.) Tem 4 Sixteen photographs from crime scene Item5 Digital video diso Ttem 6 Digital video disc ‘Due to the limited volume and generally degraded nature of the biological specimens submitted, quantitation of the identified drugs was not performed. Item 11 (blood): This sample was received with low volume, high viscotity and was degraded. Consequently, in order to affect any analyses the sample was diluted with five milliliters of laboratory grade deionized water. This dilution of the sample raised the limits of detection for all analyses pesformed on Item 11. Items 1, 7 and 8 (clothing and bed sheet/cover): Portions of these items were selected for analysis. The analytical results expressed above refer only to the portions of those iteras that ‘were sampled. Items 9 and 10 (stomach contents/small intestine/organ/viscera): Portions of these items, ‘were selected for analysis. The analytical results expressed above refer only to the portions of those items that were sampled. : Analysis for snake venom is not currently performed by the FBI Laboratory. Page 40f5 2015-00295-2 , UNCLASSIFIEDUNCLASSIFIED Remarks: Alprazolam (Alprax, Xanax) is a prescription sedative, typically prescribed for the relict of anxiety, insomnia and other disorders. Hydroxychloroquine (Plaquenil, HCQS-200) isa prescription antimalarial drug tat is ‘also used to reduce inflammation in the treatment of rheumatoid arthritis. Lidocaine is a common local anesthetic and antiarrhythmic medication. Lidocaine is used topically to relieve itching, burning, and pain from skin inflammations, injected as a dental anesthetic, or used as a local anesthetic for minor surgery. Nicotine is an active component of tobacco products and is commonly found in environments that have been exposed to tobacco smoke. Cotinine is the primary metabolite of nicotine, For questions about the content of this report, please contact Forensic Examiner Roman P. Karas at 703-632-7861. For questions about the status of your submission, including any remaining forensic examinations, please contact Request Coordinator Douglas A. Hildebrand at 703-632-838) The evidence will be retumed to the contributor under separate cover. The supporting récords for the opinions and interpretations expressed in this report are retained in the FBI files. This report contains the opinions and interpretations of the examiner(s) who issued the report. Roman P. Karas Chemistry Unit Page Sof 5 2015-00295-2 UNCLASSIFIEDFor Official Use Only Law Enforcement Sensitive Nuclear Forensic, Analysis Center ANALYSIS RESULTS — Revision 1 Radiochemistry Analysis DATE: 05 August, 2015 CASE NUMBER: 163A-NL-6093936 FBI LABORATORY NUMBER: 2015-00295 PERFORMING ORGANIZATION: Savannah River National Laboratory (SRNL), Aiken, SC 29808 PROGRAM POINT OF CONTACT: Jim Wong, 803-725-5721. jim.wong@smnl.doe.gov ‘TECHNICAL POINT OF CONTACT: John Young, 803-725-7279, John.young@srnl.doe.gov CBRNSU POINT OF CONTACT: Dr. James Blankenship, CBRNSU, 703-632-7624 Introduction and Backeround ‘The Federal Bureau of Investigation (FBI) contacted Savannah River National Laboratory (SRNL) on Wednesday, 8 July 2015, and requested assistance in the shipment and analysis of potentially radioactive evidence. Evidence was picked up at the Bertram Road Federal Express. facility on Friday, 17 July-2015. Chain of custody was initiated upon receipt and maintained throughout the analysis and storage of the items. Chemical, Biological, Radiological and Nuclear Sciences Unit (CBRNSU) inventoried all items prior to delivery to SRNL from Quantico. Alll items received were liquid, and SRNL took a 50 j4L aliquot from each item for analysis. The aliquots from FBI 9-1, 10-1, 11-1 and 12-1 (NFAC-15-245-1-1 through 245-1-4, respectively) were analyzed by the SRNL Nuclear Forensic Analysis Center (NFAC) for quantification of alphe/beta radioactivity levels by Liquid Scintillation Counting (LSC). Page 1 of3For Official Use Only Law Enforcement Sensitive ‘The results of the analysis and the final Radiological Protection Department inspection of the PB) Items are listed below in Table |. Table 1: Gross Measured Alpha and Beta of FBI Items LSC FBI Probe | Smear i Item NFACID tarmgne | Woman eee a) 200 <200, U7 0 SV | 1524-11 | Soap | cooop | _<4538 <200 <20a 4.70 0-1 -245-| ! 82612 1 008 |_ * [atprazolara, Iby the caffeine lidocaine Jdeceased Page 3 of § 2015-00295-5 UNCLASSIFIEDUNCLASSIFIED Remarks: For questions about the content of this repor, please contact Forensic Exeminer Roman P. Karas at 703-632-7861. For questions about the status of your submission, including any remaining forensic examinations, please contact Request Coordinator Douglas A. Hildebrand at 703-632-8381. ‘The evidence will be returned to the contributor under separate cover. “The supporting records for the opinions and intecpretations expressed in this report are reteined in the FEI files. This report contains the opinions and interpretations of the examiner(s) ‘who issued the report Roman P. Karas Chemistry Unitsnnent af Justice Criminal Division Office of International Affairs 20530 MDRRTIMORAT mdb Washington, 120) 4182-43697 Deseinter 7, 2015 ViA FEDERAL EXPRESS j S.K, Ahuja Under Secretary to the Government of Indie Inlornal Security-II Division, Ministry of Home Affe Hall-B), 1st Floor, NDCC-II Building, ‘Near Jantar Mantay, Jai Singh Road, ‘New Dethi - 110001 Re: Reatest for Assistance in the Matter of Case No, 04/2015 dated 01.1.20015. PS. Sarolisi Dew Under Secretary Ahuja: With regard to your request letter dated December 3,201, we provide the following information and clarification. The evidence, which we previously sent to your office, consists ofthe following: = 1. FBI Laboratory Toxicology Report on Sunanda Pushker Theroor, dated September 9, 2015; 2, Results of Radiochemistry Analysis conducted under the auspices of Dr, James F, Blankenship of the CBRNSU, deted August 5, 2015; and Supplemontal FBI Laboratory Toxicology Report on; Sunanda Pushkar Theroor, daied November 6,2015.\7 ‘The above referenced reports are the only reports prepared by the FB! laboratories in this case. to the supplemental toxicology report, dated Ootober 1, 2015, the preparer mistakenly entered the wrong, date forthe radiological (nuclear isotope testing) testing report. There is no nuclear isotope testing report ated September 3, 2015, only the report listed as item two above and dated August 5, 2015, ‘Additionally, the testing indicaies that chere were no significaot sourses of radioactivity, which preeludas ‘the presence of detectable levels of polonium 210. We regret any confusion that may have been caused, by the mistaken date eatry in the supplemental report. Pursuant to Article 7 of the Treaty, authorization of the use of this materials limited to the puurposes for which assistance has been requested and granted, Should you anticipate the need to use the rraterials for any other purpose (ie., a use not siated in the request for assistance andlor not approved in the grant of assistance), please consult this office to ascertain whether authorization or approval ean be secured.: Ifyou have any questions, please do not hesitate to contsct me by telephone m (202) 383-7384 or Sy email at markteltitpori@usdal pov. In my absences, please contact Mark de Barros by e-mail at, marke barosfusdol gov or by lelephore at (202) 397-1357. Sincerely, Mary D. Redriguez Acting Director By: vi ; Trial Atiomey