Grouping of the animals for the study:

Group I: Normal Control (No PCOS) Receiving normal diet, No Flaxseeds or

Chia seeds
Group II: Disease control Inducing PCOS with Letrozole treatment /no treatment
Group III: Drug control Receiving drug treatment with clomiphene citrate
Group IV: Receiving Flaxseed powder at a concentration of 30%
Group V: Receiving Flaxseed powder at a concentration of 40%
Group VI: Receiving Chia seed powder at a concentration of 30%
Group VII: Receiving Chia seed powder at a concentration of 40%
Group VIII: Receiving Flaxseed powder (20%) and Chia seed powder (20%)

Statistical analysis for the following groupings:

I. Analyzing the efficacy of flaxseeds on PCOS:
1. Comparison of Group I with Group II for the onset of the disease PCOS
2. Comparison between Group II and Group III ( drug - Clomiphene citrate)
3. Comparison between Group II and Group IV ( 30% flaxseeds)
4. Comparison between Group II and Group V ( 40% flaxseeds)
5. Comparison between Group IV ( 30% flaxseeds) and Group V ( 40% flaxseeds)
6. Comparison between Group III ( drug) and Group IV ( 30% flaxseeds)
7. Comparison between Group III ( drug) and Group V ( 40% flaxseeds)

II. Analyzing the efficacy of Chiaseeds on PCOS:

1. Comparison of Group I with Group II for the onset of the disease PCOS
2. Comparison between Group II and Group III ( drug - Clomiphene citrate)
3. Comparison between Group II and Group VI ( 30% chia)
4. Comparison between Group II and Group VII ( 40% chia)
5. Comparison between Group IV ( 30% chia) and Group V ( 40% chia)
6. Comparison between Group III ( drug) and Group IV ( 30% chia)
7. Comparison between Group III ( drug) and Group V ( 40% chia)

II. Analyzing the efficacy of a combination of Flaxseeds and Chiaseeds on

PCOS:
1. Comparison of Group I with Group II for the onset of the disease PCOS
2. Comparison between Group II and Group III ( drug - Clomiphene citrate)
3. Comparison between Group IV ( 30 % flax) and Group VIII ( combination)
4. Comparison between Group V ( 40% flax) and Group VIII ( combination)
5. Comparison between Group VI ( 30% chia) and Group VIII ( combination)
6. Comparison between Group VII (40% chia) and Group VIII (combination)
7. Comparison between Group III ( drug) and Group VIII ( combination)

II. Analyzing the efficacy of a combination of Flaxseeds and Chiaseeds on

PCOS:
1. Comparison of Group I with Group II for the onset of the disease PCOS
2. Comparison between Group II and Group III ( drug - Clomiphene citrate)
3. Comparison between Group IV ( 30 % flax) and Group VIII ( combination)
4. Comparison between Group V ( 40% flax) and Group VIII ( combination)
5. Comparison between Group VI ( 30% chia) and Group VIII ( combination)
6. Comparison between Group VII (40% chia) and Group VIII (combination)
7. Comparison between Group III ( drug) and Group VIII ( combination)

IV. Analyzing the comparative efficacy of Chia and Flax on PCOS:

1. Comparison of Group I with Group II for the onset of the disease PCOS
2. Comparison between Group II and Group III (drug - Clomiphene citrate)
3. Comparison between Group IV ( 30 % flax) and Group VI ( 30% chia)
4. Comparison between Group V ( 40% flax) and Group VII (40% chia)