Must know in Mycology

Basic structure of fungi hyphae and spore

General culture media SDA
Czapeks medium Aspergillus
Serum at 37OC germ tube
Cornmeal agar Chlamydospore
Media with olive oil Malassezia furfur
Hair baiting test T. mentagrophytes
Trichophyton skin, hair, nails
Red color, tear drop conidia T. rubrum
Favic chandelier (antler) T. schoenleinii
Coenocytic Phycomycetes / Zygomycetes
Ascospore Saccharomyces cerevisiae
C. neoformans Niger birdseed (+), urease (+), phenol oxidase (+), India ink
Arthrospore Geotrichum, Coccidioides, Trichosporon, Aureobasidium
Blastospores yeast
10% KOH direct microscopic, clearing
LPCB common fungal stain
Wright / Giemsa H. capsulatum
Dichotomous hyphae Aspergillus
Thrush, diaper rash, Moniliasis Candida
Actinomyces Sulfur granules
Nocardia modified acid fast
Macro- microconida Dermatophytes
Sputum systemic mycoses
CHO assimilation ID yeast, free of CHO
BHIA- dimorphic fungi
C. immitis high risk to lab personnel
Chlorampenicol and cycloheximide inhibitory agent for mycosel / mycobiotic