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Journal of Ethnopharmacology
journal homepage: www.elsevier.com/locate/jep
a r t i c l e i n f o a b s t r a c t
Article history: Ethnopharmacological relevance: Lauha bhasma (iron ash) is one of the iron-based herbo-metallic
Received 20 September 2011 preparations used in Ayurvedic medicine for treating various ailments due to iron deciency.
Received in revised form Materials and methods: The preparation of Lauha bhasma (iron ash) requires normal purication (heat
25 March 2012
treatment in vegetable and animal products), special purication (treatment with herbal constituents)
Accepted 10 April 2012
Available online 26 April 2012
and calcination steps aimed at converting the raw material to a suitable therapeutic form. In this study,
we have systematically and scientically evaluated through a series of qualitative tests and modern
Keywords: analytical tools the importance of the treating media.
Lauha bhasma (iron ash) Results: Our data demonstrates that these steps are necessary to remove the grease and scales in the
Ayurveda (knowledge of life)
raw material. While heating, microcracks appeared on the surface of the iron, which improved the
Herbo-metallic preparation
reactivity with the herbal constituents in addition to incorporating nanostructured features. Further,
Nanostructure
the use of plant products facilitated the removal of Fe3 present in the raw material by forming soluble
complexes. The Fe2 present in the raw materials also forms an insoluble complex with the herbal
constituents in the presence of UV radiation.
Conclusions: In conclusion, our data summarily suggest that the purication steps involved in the
preparation of Lauha bhasma (iron ash) are critical.
& 2012 Elsevier Ireland Ltd. All rights reserved.
0378-8741/$ - see front matter & 2012 Elsevier Ireland Ltd. All rights reserved.
http://dx.doi.org/10.1016/j.jep.2012.04.021
B. Krishnamachary et al. / Journal of Ethnopharmacology 142 (2012) 98104 99
modify the properties of the therapeutic materials to enhance sodhana (special purication), bhanupaka (exposure to sunlight),
their potential. sthali paka (roasting in an iron pan) and putapaka (calcination). A
The traditional Ayurvedic practitioners have used various ow diagram depicting the steps in the preparation of Lauha
qualitative tests to characterize the bhasma (ash), which includes bhamsa (iron ash) is shown in Fig. 1.
varna (test for color), nishchandratvam (test for luster), varitara
(test for lightness), rekhapurnata (test for neness), niruttha (test
2.1.1. Samanya sodhana (normal purication)
for metallic form) (Anand and Neetu, 2010). Many variations in
The rst step in the preparation of Lauha bhasma (iron ash) is
the procedure for the preparation of Lauha bhasma (iron ash) have
sodhana (purication step), which involves sequential quenching
been reported in the literature (Singh and Reddy, 2011; Bhanu,
in tila thaila (sesame oil), takra (butter milk), kanjika (rice gruel),
1997). Hence it is imperative to understand the scientic basis of
gomuthra (cows urine), and kulatha kasaya (horse gram decoc-
various steps involved in the preparation of Lauha bhasma (iron
tion). As in a typical purication step, about 2 kg of the raw
ash). In this study, we have systematically and scientically
material (iron lings) was heated to red hot condition ( 530
evaluated the importance of each of the purication steps
560 1C) and immersed in 2 L of quenching medium (room tem-
involved in the preparation of Lauha bhasma (iron ash).
perature) for 2 h. Each of the quenching processes was repeated
thrice with each treating liquid by using fresh medium every
time. These steps constitute samanya sodhana (normal purica-
2. Materials and methods
tion) and the Lauha (iron) obtained at this stage is called Suddha
Lauha (iron after normal purication).
2.1. Preparation of Lauha bhasma (iron ash)
The preparation of Lauha bhasma (iron ash) was carried out 2.1.2. Vishesha sodhana (special purication)
following the procedure described in the Ayurvedic Formulary of Coarse powders of dried fruits, viz., Phyllanthus emblica (Indian
India. The raw material (Iron lings) were procured from the gooseberry), Terminalia chebula (Chebulic myrobalans) and Termi-
market in Trichy, India and used as raw material for preparation nalia bellerica (Belleric myrobalans) were taken in equal quanti-
of Lauha bhasma (iron ash). The preparation involves the follow- ties (2 kg each) and mixed with 50 L of water. The resulting
ing major stepssamanya sodhana (normal purication), vishesha mixture was heated to reduce to one-eighth of the original
Fig. 1. Flow diagram showing various steps in the preparation of Lauha bhasma.
100 B. Krishnamachary et al. / Journal of Ethnopharmacology 142 (2012) 98104
volume to obtain Triphala kasaya (decoction of three fruits). About cool to room temperature. When this process is repeated number of
2 kg of Suddha Lauha (iron after normal purication) was heated times, it is referred in the classical ayurveda texts as bhasmikarana
to red-hot condition and immersed in a mixture of 1 L each of (ashing). For the puta (calcination) process, the cakrikas (thin at
triphala kasaya (decoction of three fruits) and cows urine for 2 h. disks) were placed in an earthen vessel (Fig. 2(A)) and covered with
This purication step was repeated seven times using freshly another earthen vessel (Fig. 2(B)) and the interface between the
prepared mixture of triphala kasaya (decoction of three fruits) and vessels was sealed with a clay-smeared cloth (Fig. 2(C)). This
cows urine. The Lauha (iron) obtained at this stage is called Lauha arrangement is known as sarava samputa (sealed earthen pot).
after vishesha sodhana (iron after special purication). The calcination process in the preparation of Lauha bhasma (iron
ash) was carried out in a specially designed pit, measuring 90 90
2.1.3. Bhanupaka (exposure to sunlight) 90 cm. Cow dung cakes each weighing about 350 g were used as heat
Triphala kasaya (decoction of three fruits) was added to Lauha source. The bottom half of the pit was stacked with several layers of
obtained after vishesha sodhana (iron after special purication) cow dung cakes, over which the sarava samputa (sealed earthen pot)
and allowed to dry under sunlight for 5 day. This process was containing cakrikas (thin at disks) were placed (Fig. 3). This was
repeated seven times and the Lauha at this stage is called Lauha followed by stacking several layers of cow dung cakes on top. The
after bhanupaka (puried iron after exposure to sunlight). number of cow dung cakes used in this process ranged between 250
and 300 and this arrangement is referred to as Gajaputa (calcination
in pit measuring one yard in three directions) (Fig. 3).
2.1.4. Sthalipaka (frying in iron pan)
Triphala kasaya (decoction of three fruits) was freshly prepared 2.2. Characterization
by adding 2.3 kg each of Indian gooseberry, Chebulic myrobalans
and Belleric myrobalans to about 110 L of water and reduced to In order to understand the stage-wise transformations in
one-eighth of its original volume. About 2.3 kg of Lauha after surface morphology, the solid material obtained after each
bhanupaka (puried iron after exposure to sunlight) was washed quenching step was characterized using a cold-eld emission
with water and placed in a sthali (iron pan), to which 13.5 L of scanning electron microscope (FE-SEM, JSM 6701F, JEOL, Japan).
Triphala kasaya (decoction of three fruits) was added and heated The crystallinity of the samples obtained after each purication
to dryness for about 1 h at 95100 1C. The solid material obtained step was analyzed using an X-ray diffractometer (D8 Focus,
after this purication process is referred to as Lauha after Bruker, Germany). The surface area of samples was determined
sthalipaka (puried iron after frying in iron pan). using BET isotherms obtained from a surface area analyzer (ASAP
2020, Micromeritics, USA). The infrared spectrum and the elec-
2.1.5. Puta paka (calcination) tronic spectrum were recorded using a FTIR analyzer (Perkin
About 670 g each of Indian gooseberry, Chebulic myrobalans Elmer, Germany) and UV-Visible Spectrometer (Lamda 750,
and Belleric myrobalans were added to 4 L of water and heated to Perkin Elmer, USA) with diffuse reectance accessory, respectively.
reduce to one-fourth of its original volume. This was added to
2 kg of Lauha after sthalipaka (puried iron after frying in iron 2.3. Potassium thiocyanate (KCNS) test for presence of Fe3
pan); triturated well and made into cakrikas (thin at disks),
which were dried and stored for the next transformation. About 1 mL of KCNS was added to 1 mL of sample to test for
In traditional literature, the process of puta (calcination) refers to the appearance of red color. Immediate appearance of red color
controlled heating of herbo-mineral preparations and allowing to indicates the presence of free iron in trivalent form. In the
Fig. 2. Arrangements for Sarava samputa [A] cakrikas in an earthen vessel; [B] earthen vessels before sealing and [C] interface sealed with clay-smeared cloth.
Fig. 3. Arrangements for Gajaputa [A] Sarava samputa over stacked cow dung cakes; [B] Sarava samputa sandwiched between layers of cow dung cakes.
B. Krishnamachary et al. / Journal of Ethnopharmacology 142 (2012) 98104 101
absence of red color, few drops of concentrated nitric acid were oil before and after being used for purication. The spent sesame
added to decompose the complex and liberate free Fe3 and KCNS oil appeared dark in color due to transfer of greasy matter from
was added to test for the appearance of red color. iron. Fig. 5 shows the scanning electron micrographs of raw iron
and sesame oil-treated iron, demonstrating that the raw material
2.4. Photochemical reactor does not possess crystallites or grains in nanoscale ( o100 nm),
while several nanostructures were observed in the size range of
A photochemical reactor was designed to study the effect of UV
50100 nm for treated iron, which may be attributed to the
radiation during the process of bhanupaka (exposure to sunlight)
physical properties and temperature of the quenching medium.
(Fig. 4). A mixture of 0.4 g iron (III) chloride, 20 mL of triphala
High boiling liquids like vegetable or mineral oils are generally
kasaya (decoction of three fruits) and gallic acid (1 g) were placed
preferred over aqueous media for quenching due to the limita-
in a quartz tube and irradiated with 125 W UV source. After 2 h,
tions of the latter like inefcient heat transfer due to excessive air
the sample was checked for the presence of solid content.
bubbles formed on the surface. On the other hand, quenching in
cold oil renders more brittleness to the product than that in hot
3. Results and discussion oil due to the faster rate of cooling in the former case (Bhanu,
1997). In a similar trend, repetitive quenching (e.g., three times)
3.1. Heat treatment with sesame oil might induce more thermal stress causing cracks and fractures
thereby reducing the particle size.
Sesame oil is used to remove greasy matter from the raw
material. Table 1 summarizes the characteristics of fresh sesame
3.2. Heat treatment with buttermilk
Table 1
Characteristics of fresh sesame oil and sesame oil after treatment.
Fig. 5. Scanning electron micrographs of raw material and sesame oil-treated material.
102 B. Krishnamachary et al. / Journal of Ethnopharmacology 142 (2012) 98104
Table 2
BET surface area of solid material after each treatment step.
have also been observed due to these heat treatments (Fig. 7),
depending on parameters including chemical composition of
treating liquid, temporal variation of temperature and surface
energy.
Fig. 7. Surface morphology of solid material after each treatment step during samanya sodhana.
Fig. 8. Surface morphology of Lauha after bhanupaka and Lauha after Sthalipaka.
(Pineda and Ashmead, 2001). Chelated iron is less toxic than free 4. Conclusions
and hydrated metallic ions (Papanikolaou and Pantopoulos, 2005;
Heather et al., 2003). Hence, it is imperative to have iron in the The process of making Lauha bhasma (iron ash) as per the
divalent state to enter mucosal cells. Fe3 form is not preferred as Ayurvedic texts involves thorough and stringent purication steps
it has to be reduced to ferrous form in the intestinal lumens, which include the samanya sodhana (normal purication), vishe-
which is difcult. sha sodhana (special purication), bhanupaka (exposure to
104 B. Krishnamachary et al. / Journal of Ethnopharmacology 142 (2012) 98104
References