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A viable cell count is essential to evaluate the kinetics of cell growth and is crucial for the study
of eukaryotic cells for different purposes. The suspension is either spread onto the surface of agar
plates (spread plate method), or is mixed with molten agar, poured into plates, and allowed to
solidify (pour plate method). 0.1 mL of the diluted culture with dilution factor 106, 107 and 106
are transferred to each agar plate respectively. Streaking technique using bent glass spreader is
used to distribute the bacteria on the surface of agar plate to ensure evenly bacterial growth.
(Cadena-Herrera et al., 2015). The number of colonies on a dilution plate showing a range
between between 30 and 300 colonies is considered statistically effective. Fewer than 30
colonies makes a poorer statistical precision while greater than 300 colonies often
results in overlapping colonies and imprecision in the count. (Cadena-Herrera et al., 2015)
(Sieuwerts et al., 2008)
Ben-David, A. and Davidson, C. E. (2014) Estimation method for serial dilution experiments,
Journal of Microbiological Methods, 107, pp. 214221. doi: 10.1016/j.mimet.2014.08.023.
Cadena-Herrera, D. et al. (2015) Validation of three viable-cell counting methods: Manual, semi-
automated, and automated, Biotechnology Reports, 7, pp. 916. doi:
10.1016/j.btre.2015.04.004.
Keler, C. et al. (2010) Serial Dilution Simulation Lab, The American Biology Teacher, 72(5), pp.
305307. doi: 10.1525/abt.2010.72.5.9.
Sieuwerts, S. et al. (2008) A simple and fast method for determining colony forming units,
Letters in Applied Microbiology, 47(4), pp. 275278. doi: 10.1111/j.1472-765X.2008.02417.x.