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BACHELOR OF SCIENCE IN MEDICAL TECHNOLOGY

Incidence of Orthostatic Proteinuria among selected

Fish vendors at Tanauan City Public Market

A Thesis presented to the

College of Allied Medicine
In partial fulfillment of the requirements for the degree
Bachelor of Science in Medical Technology

Manaig, Shaira Alheena Mikaili O.

Nuevo, Marianne Carmel M.
Zumarraga, Lauri Leigh Q.

May 2017
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APPROVAL SHEET

This research paper entitled Incidence of Orthostatic Proteinuria among selected Fish

vendors at Tanauan Public Market, prepared and submitted by Manaig, Shaira Alheena

Mikaili O., Nuevo, Marianne Carmel M., and Zumarraga, Lauri Leigh Q., in partial

fulfillment of the requirements for the degree of BS in Medical Technology has been

examined and is recommended for acceptance and approval for Oral Defense.

Unice L. Padilla, RMT

Adviser

Approved by the Committee on Oral Examination.

Leah F. Quinto, RMT, MSc Dr. Merlita C. Medallon

BS Medical Technology Chairperson Statistician, Research Center
(Panel Member) (Panel Chair)

Accepted and approved in partial fulfillment of the requirements for the degree of Bachelor
of Science in Medical Technology.

Janet C. Calupitan, RN, MAN

Dean, College of Allied Medicine
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ABSTRACT

The study Incidence of Orthostatic Proteinuria among selected Fish vendors in Tanauan
Public Market is mainly about the occurrence of Orthostatic Proteinuria among the
selected respondents who are prone to be affected of the said condition due to prolonged
standing. Thirty Tanauan Fish vendors were chosen for they are one of the most suitable
respondents for this study. Urine samples with a volume of forty mL are collected from
the respondents before standing and after prolonged standing, placed in a sterile container
and stored in an ice box to preserve and maintain the specimens integrity. Reagent strips
are used to test for the presence of protein while Sulfos alicyclic Acid is the reagent used
as a confirmation for the presence of protein in which each trial, before and after
prolonged standing results are recorded. The study has shown that there is an incidence of
Orthostatic Proteinuria among the selected respondents for having positive results in all
the three trials specifically during the 3rd trial of the experiment. There is no significant
difference in their protein level when grouped by age and gender, but with regards to
hours of standing, a significant difference was observed. Study has also showed that there
is a significant relationship between the protein, Sulfosalicylic Acid and the hours of
standing. Therefore, researchers proved that as hours of standing are prolonged, an
increase number of respondents showed positive results in their protein level and
sulfosalicylic acid test.

Keywords: Orthostatic Proteinuria, Sulfosalicylic Acid, Reagent Strip, Urine protein

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ACKNOWLEDGEMENT

The researchers would like to extend their deepest gratitude to the Father

Almighty who gave them knowledge and ability to to finish this kind of academic

endeavor.

To Ms. Unice L. Padilla, RMT the researchers thesis adviser, for her guidance

throughout this roller coaster ride journey and for the encouragement to strive excellence.

To Ms. Ana Maria A. Bacudio, RMT, MPH the researchers research professor,

for her expertise and strength of character in the pursuit of academic excellence.

To Dr. Merlita C. Medallon, Director of Research and Statistical Center, for

sharing her expertise in the field of Statistics.

To Ms. Madelle Abuyo, the researchers grammarian, for sharing her expertise in

checking this paper and for her efforts to make this paper more universally acceptable.

To all the fish vendors that served as their respondents in this study, the

researchers are extending their utmost gratitude for your full cooperation in their data

gathering.

To the researchers parents, for the patience and understanding, assistance and

unwavering support. It is impossible to adequately acknowledge the support and

encouragement provided by them.

Upon the completion of this study, the researchers humbly thank all the

individuals who have played roles in making this possible. It is not possible to name all

of the individuals who have contributed to this research study. To those mentioned here
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and to those not explicitly named heartfelt thanks for your phenomenal efforts and

support.
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TABLE OF CONTENTS
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TITLE PAGE i
APPROVAL SHEET ii
ABSTRACT iii
ACKNOWLEDGEMENT iv
TABLE OF CONTENTS vi
LIST OF TABLES viii
LIST OF FIGURES viii

CHAPTER

1 INTRODUCTION 1
Review of Literature 3
Conceptual Framework 12
Objectives of the Study 13

2 RESEARCH METHODOLOGY 14
Research Design 14
Research Locale 14
Respondents of the Study 15
Sources of data 15
Instrumentation and validation 16
Collection of samples 16
Research Methods 17
Statistical Treatment 17
Standards 19
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3 RESULTS AND DISCUSSION 20

4 CONCLUSION AND RECOMMENDATION 27

Conclusions 27
Recommendations 28

BIBLIOGRAPHY 29
Appendices
A. Definition of Terms 34

B. Letter of Permit 35

C. Informed Consent 38

D. Interview Questions 40

E. Materials and Procedures 42

Curriculum Vitae 45
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LIST OF TABLES

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Table 1. Frequencies and Percent Distribution of the 20

Respondents According to Age
Table 2. Frequencies and Percent Distribution of the 21
Respondents According to Hours of standing
Table 3. Protein of urine before and after prolonged 22
standing using Reagent Strip
Table 4. Sulfosalicylic Acit test result of urine before 23
and after prolonged standing
Table 5. Significant difference of protein when grouped 24
in terms of Age/ Gender/ Hours of standing
Table 6. Pearson Correlation of Protein 26

LIST OF FIGURES
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Figure 1. Representation of the Diagram Procedure 18