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Polysaccharide-based chiral HPLC

The moment you adopt our Kromasil High Performance Concept, you join thousands
phases for better performance

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of chromatographers who share a common goal: to achieve better separations when
analyzing or isolating pharmaceuticals or other substances.
Not only will you benefit from our patented silica technology, but you gain a
strong partner with a reliable track record in the field of silica products. For the past
60 years, Eka Chemicals has pioneered new types of silica. Our long experience in

This publication may not be reproduced in any way without the consent of Eka Chemicals AB.
the field of silica chemistry is the secret behind the development of Kromasil, and
the success of our Separation Products Group.
Kromasil is available in bulk, or in high-pressure slurry-packed columns.
The development, production and marketing of Kromasil are ISO 9001 certified.
Eka Chemicals is a global company with 3,000 people in 30 countries. It is a business
unit within AkzoNobel, one of the worlds largest chemical groups, with more than
67,000 employees in 80 countries.

Eka Chemicals AB 2011

Kromasil AmyCoat
Kromasil CelluCoat

www.kromasil.com Kromasil
Designed to stretch the limits.

Kromasil AmyCoat and Kromasil CelluCoat stretch the limits for analytical
and industrial chiral chromatography. The silica is based on an in-house developed
matrix and coated with a functionalized amylose or cellulose selector. High resolution,
excellent selectivity, no pressure limits* and stable performance when switching
between compatible mobile phases are some important benefits.

Analytical chromatography

No pressure limits High flow rates Faster analyses

Small particles (3 m) High resolution Better analyses

Unique coating method Excellent selectivity Better analyses

Preparative chromatography

Unique silica matrix Mechanically strong Longer lifetime

Wide range of particles Consistent performance Easier to scale up

Unique coating method Higher loadability Higher productivity

* Kromasil AmyCoat and Kromasil CelluCoat can withstand flow rates equivalent to
pressures of up to 400 bari.e. the approximate limit for most HPLC systems.
Peak performance. Expect nothing less. Kromasil AmyCoat Kromasil CelluCoat
Selectivity and resolution comparisonKromasil AmyCoat 3 m and Chiralpak AD-H (5 m). Selectivity and resolution comparisonKromasil CelluCoat 3 m and Chiralcel OD-H (5 m).
Kromasil AmyCoat, 3 m Chiralpak AD-H
Rs Kromasil CelluCoat, 3 m Chiralcel OD-H
Rs
To speed up and simplify method development, we have removed some of the 500
= 1.5
800 0
= 1.5
AmyCoat 3 m Chiralpak AD-H* AmyCoat 3 m Chiralpak AD-H* 500
= 2.3
400 0
= 2.0
CelluCoat 3 m Chiralcel OD-H* CelluCoat 3 m Chiralcel OD-H*

00
Rs= 7.3 605 0 Rs= 5.8 Ambucetamide 1.4 1.4 4.8 4.2 Rs= 13.2 305 0 Rs= 8.3 Trans-Stilbene oxide 2.3 2.0 13.2 8.3
restrictions for coated polysaccharide phases. In analytical scale chromatography, 000 Carbinoxamine 1.5 1.5 7.3 5.8
50
0 Benzoin 1.5 1.5 8.6 5.7
400 0 Ketoprofen 1.4 1.3 4.6 4.3 200 0 TFAE 2.9 2.9 14.7 11

3 m particles and the absence of pressure limits allow faster chromatography with 0
50
Naproxen 1.2 1.2 3.4 3.1 00
0
Trgers base 1.4 1.4 3.7 2.7
205 Oxamniquine 1.2 1.2 3.3 3.1 105 Oxprenolol 5.6 5.5 18.1 15.1
50
better results than ever before. 0 0
Proglumide
Sulindac
2.7
1.3
2.8
1.3
11.8
4.8
9.0
3.9 0 0
Naproxen
Proglumide
1.2
2.0
1.2
2.0
2.9
7.6
2.2
3.2
0 5 10 15 0 5 10 15 (* 5 m) 0 2 4 6 8 0 2 4 6 8 * (5 m)
[min.] [min.] [min.] [min.]

Common conditions Common conditions


Solute: Carbinoxamine Flow rate: 0.5 ml/min Solute: trans-Stilbene oxide Flow rate: 1 ml/min
Mobile phase: Heptane/2-Propanol/DEA (90/10/0.1) Temperature: 22 C Mobile phase: Heptane/2-Propanol (90/10) Temperature: 25 C
Better results Faster results Column size: 4.6 150 mm Detection: UV 226 nm Column size: 4.6 x 150 mm Detection: UV 229 nm

Both Kromasil AmyCoat and Kromasil CelluCoat Not only can you get better results with Kromasil
show great enantioselectivity for many different AmyCoat and Kromasil CelluCoat you also get
racemates. In a separate section of the brochure, them faster. Thanks to a product characteristic
you will find an application guide showing exactly like the absence of pressure limits, you can run
the performance levels you can expect. analytical chromatography at very high flow
By having access to 3 m particles, it enables rates and save time. Difference in resolutionKromasil AmyCoat Fast analytical chromatography Fast analytical chromatography
a higher plate count and resolution for analytical The high selectivity and 3 m particles are also 3 m vs. 5 m.
chromatography. Combined with great selectivity, important factors in speeding up the method. You Particle size: 5 m Particle size: 3 m Particle size: 3 m Particle size: 3 m Particle size: 3 m Particle size: 5 m Particle size: 3 m Particle size: 3 m Particle size: 3 m Particle size: 3 m
Column length: 150 mm Column length: 150 mm Column length: 150 mm Column length: 150 mm Column length: 150 mm Column length: 250 mm Column length: 150 mm Column length: 50 mm Column length: 50 mm Column length: 50 mm
this facilitates the separation of the enantiomers. achieve an improved resolution, which, in turn, .4
4
Flow rate: 1 ml/min
.5
Flow rate: 1 ml/min Flow rate: 1 ml/min Flow rate: 2 ml/min Flow rate: 4.5 ml/min Flow rate: 1 ml/min Flow rate: 1 ml/min Flow rate: 1 ml/min Flow rate: 3 ml/min Flow rate: 7 ml/min

0
allows a higher flow rate. 0 = 1.1 = 1.1 00 00 00 50 00 00 00 5
.3
.4 =2.3
= 1.8
= =2.21.8 = 1.7 = 2.2 = 2.3 = 2.3 = 2.2 = 2.2
Rs= 1.3 Rs= 2.1 Rs=R4.7
= 6.9 R = 5.6 Rs= 3.5
00 s 00 Rs=s 3.8 Rs= 9.0 Rs= 10.8 Rs= 4.7 Rs= 3.8 Rs= 3.0
00 50 50
0 .3 00 00 0 0
.2 00 00
.2 00 00 00
00 00
0
.1 50 00 5 0
.1 00 50 50
00 00

0 0 0 0 0 0 0 0 0 0
0 2 4 6 0 1 2 3 0 0.75 1.5 0 5 10 0 5 10 0 1 2 3 0 1 0 1
[min.] [min.] [min.] [min.] [min.] [min.] [min.] [min.]

Common conditions Common conditions Common conditions


Solute: trans-2-Phenyl-1-cyclohexanol Temperature: 25 C Solute: Trgers base Temperature: 25 C Solute: trans-Stilbene oxide Temperature: 25 C
Mobile phase: Heptane/2-Propanol (95/5) Detection: UV 220 nm Mobile phase: Heptane/2-Propanol/DEA (90/10/0.1) Detection: UV 220 nm Mobile phase: Heptane/2-Propanol (90/10) Detection: UV 229 nm
Column diameter: 4.6 mm Column diameter: 4.6 mm Column diameter: 4.6 mm

Baseline separation by Baseline separation in Baseline separation in


using 3 m particles less than 1 minute less than 30 seconds

Kromasil AmyCoat and Kromasil CelluCoat are Kromasil AmyCoat and Kromasil CelluCoat
available in particle sizes down to 3 m. faster results, better results.
CelluCoat high flow rate

Makes everyday work so much easier. Kromasil AmyCoat Kromasil CelluCoat


Stable performanceNo pressure limitsFreedom to switch solvents Stable performanceNo pressure limitsFreedom to switch solvents
Identical result Identical result 120 8

selectivity

Change rel. start value [%]


7

Kromasil AmyCoat and Kromasil CelluCoat let you perform method development with-

Reduced plate height


Before After backpressure
110 6

Solute: trans-Stilbene oxide Test sequence Solute: trans-Stilbene oxide 5


= 2.2
out interference from restrictive parameters. Forget pressure limits, long equilibration
Start
00 00 100 4
0 = 3.1 Mobile phase Flow rate Run time Total back-pressure
= 3.1 After 100% Acetonitrile (3h) = 2.1
[ml/min] [h] [bar] 3

times and degradation in performance when switching from one compatible mobile 00
Rs= 15.5
1. Heptane/2-Propanol (90/10) 4.3 2 345 00
Rs= 15.6
90 2
After 100% 2-propanol (3h) = 2.1
0
1
2. 2-Propanol 0.7 2 260
phase to anotherthey simply do not exist anymore. 00 3. Acetonitrile 1 2 58 00
80
0 10 20 30 40 50
0
0

0 time [h]
1 0.1 0.2 0.3 0.4 0.5 0.6
4. Ethanol 1 2 188 Reduced velocity
0 5. Heptane/2-Propanol (90/10) 1 2 72 0 Conditions Common conditions
Solute: trans-Stilbene oxide Mobile phase: Heptane/2-Propanol (90/10) Solute: trans-Stilbene oxide Mobile phase: Heptane/2-Propanol (90/10)
0 5 10 0 5 10 Stationary phase: Kromasil CelluCoat, 3 m Flow rate: 7 ml/min Stationary phase: Kromasil CelluCoat, 5 m Flow rates: 0.1-1.2 ml/min
[min.] [min.] Column size: 4.6 x 50 mm Temperature: 25 C Column size: 4.6 x 250 mm Temperature: 25 C
No pressure limits Short equilibration times
Common conditions
User-friendliness, as we see it, is the ability to Column equilibration is a time-consuming activity Stationary phase: Kromasil AmyCoat, 3 m
Column size: 4.6 150 mm
Mobile phase: Heptane/2-Propanol (90/10)
Flow rate: 1 ml/min
Temperature: 25 C Detection: UV 229 nm
perform method development without being when running chiral chromatography. Higher flow
restricted by various parameters. One well-known rates allow Kromasil AmyCoat and Kromasil
restriction for coated polysaccharide phases is the CelluCoat to show substantially shorter equilibra-
general pressure limit over the bed. Kromasil tion times than other coated phases on the mar-
AmyCoat and Kromasil CelluCoat withstand flow ket. Short equilibration timesFreedom to switch solvents
Identical result Identical result
rates equivalent to pressures up to 400 bar In general, long equilibration times are most
which is about the limit for the HPLC system itself. pronounced when switching mobile phases Short equilibration timesFreedom to switch additives Mobile phase 1 Mobile phase 2 Mobile phase 1

50 50
50
This makes it possible to run analytical chromatog- containing basic additives to acidic additives or Identical result Identical result
00
= 2.3 00 = 1.4 00 = 2.2
Rs= 10.0 Rs= 2.6 Rs= 9.9
50 50
raphy a lot faster. the other way around. The test with a Kromasil Mobile phase 1
Solute: Carbinoxamine
Mobile phase 2
Solute: Ketoprofen
Mobile phase 1
Solute: Carbinoxamine
50
00 00
Detection: UV 228 nm Detection: UV 250 nm Detection: UV 228 nm 00 Equilibration Equilibration
CelluCoat 3 m column switching between two 00 00 00 50
Mobile phase 2 50
Duration: 10 min
Mobile phase 1 50
Duration: 15 min
= 1.5 = 1.2 = 1.5 0 0
Stable performance compatible mobile phases shows how short 00 Rs= 5.0
50
Rs= 3.4 00 Rs= 5.0
0
0 1 2 3 0 2 4
0 2 4 [min.] [min.]
[min.]
00
From a stability perspective, you can run Kromasil equilibration times you can get. 00 Equilibration Equilibration 00
Mobile phase 2 50 Mobile phase 1 Common conditions
AmyCoat and Kromasil CelluCoat using compatible There are also two tests illustrating short Duration: 18 min Duration: 12 min Solute: trans-Stilbene oxide
Detection: UV 229 nm
Column size: 4.6 x 150 mm
Mobile phase 1: Heptane/2-Propanol (90/10)
Flow rate: 2 ml/min
Temperature: 25 C
0 0 0
Stationary phase: Kromasil CelluCoat, 3 m Mobile phase 2: Ethanol
normal, polar organic and reversed mobile phases. equilibration times and additive switch for both 0 2 4
[min.]
0 2 4 6 8
[min.]
0 2 4
[min.]

Switching between compatible normal to polar Kromasil AmyCoat and Kromasil CelluCoat with
Common conditions
Stationary phase: Kromasil AmyCoat, 3 m Mobile phase 1: Heptane/2-Propanol/DEA (90/10/0.1) Flow rate: 2 ml/min
organic mobile phases will not lead to any deg- absolutely no sign of memory effects. Column size: 4.6 x 150 mm Mobile phase 2: Heptane/2-Propanol/TFA (90/10/0.1) Temperature: 25 C

radation in performance, no need for solvent


dedicated columns.
To show the stability in column efficiency for
Kromasil CelluCoat, we have performed mobile Short equilibration timesFreedom to switch additives
Identical result Identical result
phase switches and visualized them after each
Mobile phase 1 Mobile phase 2 Mobile phase 1
switch with a Knox plot. Solute: Naproxen Solute: Trgers base Solute: Naproxen
Detection: UV 226 nm Detection: UV 220 nm Detection: UV 226 nm
00
00
00 = 1.2 00
= 1.4 = 1.2
Rs= 2.7 Rs= 3.4 00 Rs= 2.6
00 00
Equilibration Equilibration 00
Mobile phase 2 Mobile phase 1
00
Duration: 20 min Duration: 20 min 00
00
00 00

0 0 0
0 2 4 6 8 10 0 2 4 6 8 0 2 4 6 8 10
[min.] [min.] [min.]

Common conditions
Stationary phase: Kromasil CelluCoat, 3 m Mobile phase 1: Heptane/2-Propanol/TFA (90/10/0.1) Flow rate: 1 ml/min
Column size: 4.6 x 150 mm Mobile phase 2: Heptane/2-Propanol/DEA (90/10/0.1) Temperature: 25 C
Works all the way. Kromasil AmyCoat Kromasil CelluCoat
Preparative applications Loadability comparison
Kromasil CelluCoat, 10 m Chiralcel OD, 10 m Kromasil CelluCoat, 10 m Chiralcel OD, 10 m

Mobile phase: Heptane/2-Propanol/DEA (90/10/0.1)


Analytical injection Preparative injection
Equivalent loading on a
Sample size: 10 g Sample size: 7.5 mg
Kromasil products are well known for their ability to work along the whole spectrum Detection: UV 254 nm Detection: UV 280 nm 50 250 mm column: Analytical injection: 20 g Analytical injection: 20 g Preparative injection: 5 mg
Collected fractions size: 0.5 min
Preparative injection: 5 mg
Collected fractions size: 0.5 min
00 00
50 60 00 00
from analytical to industrial scale chromatography. Kromasil AmyCoat and Kromasil = 1.5
00 = 1.3 = 1.2 00
40
00
CelluCoat are no exception. 00
30
40 00 00

00 00
00 20

Flow rate: 0.7 ml/min


20 00 00
00

Carbinoxamine
10 00
0 0
0 5 10 15 0 5 10 15
0.9 g 0 0 0 0
Simplifies method development 4000 [min.] [min.] 0 5 10 15 20 25
[min.]
0 5 10 15 20 25
[min.]
0 5 10 15 20 25
[min.]
0 5 10 15 20 25
[min.]
Enantiomer 1 Enantiomer 2
With particle sizes from 3 m to 10 m giving 3500
Purity [%] 100 99.7
3500100
Yield [%] 99.7 Enantiomer 1 Enantiomer 2

identical selectivity, Kromasil AmyCoat and Kromasil CelluCoat, 10 m (7.2 bar*)


Kromasil CelluCoat, 10 m (7.2 bar)*
Purity
[%]
Yield
[%]
Purity
[%]
Yield
[%]
3000 3000 Kromasil CelluCoat 91.2 73.3 94.4 50.1
Kromasil CelluCoat make it easy to scale up while Daicel Chiralcel OD 91.4 46.7 96.6 24.9

B ac k -pres s u re i n c reas e [%]


B a c k -p r e s s u r e i n c r e a s e [ % ]
Kromasil AmyCoat, 10 m (9 bar*) Analytical injection Preparative injection
Kromasil AmyCoat, 10 m (9 bar)*

Mobile phase: Heptane/2-Propanol (85/15)


Sample size: 8.5 g Sample size: 17.1 mg
retaining excellent performance. 2500 Detection: UV 230 nm Detection: UV 325 nm 2500 Common conditions
Chiralcel OD, 20 m (0.8 bar*) CHIRALCEL OD, 20 m (0.8 bar)* Solute: Naproxen Temperature: 25 C
50 00
As for all Kromasil products, you can perform 2000 0 2000
Mobile phase: Heptane/2-Propanol/TFA (90/10/0.1)
Column size: 4.6 x 250 mm
Flow rate: 0.5 ml/min

00 = 3.3 CHIRALPAK AD, 20 m (0.8 bar)*


the required method development in analytical Chiralpak AD, 20 m (0.8 bar*)
0 00
1500 50 1500

Flow rate: 1 ml/min


scale columns and then scale up to a larger column. (* initial bed pressure) 00
00
You can, for example, use 3 m particles in 1000 1000

Warfarin
50

an analytical scale column and translate all the 500 0 0 500 2.0 g
0 10 20 30 0 5 10 15 20 25 30 35
generated data to a larger column packed with [min.] [min.]
0 0
Enantiomer 1 Enantiomer 2
10 m particles. 1 2 3 4 5 6 Purity [%] 99.7 99.5 1 2 3 4 5 6
(70 bar) (70 bar) (70 bar) (70 bar) (100 bar) (100 bar)
Yield [%] 99.4 99.7
(70 bar) (70 bar) (70 bar) (70 bar) (100 bar) (100 bar) Preparative applications
If you plan to scale up the process, we suggest
Cycles of packing (piston pressure) Cycles of packing (piston pressure) Analytical injection Preparative injection
Common conditions Equivalent loading on a
you use an analytical column packed with 10 m

Mobile phase: Heptane/2-Propanol (90/10)


Stationary phase: Kromasil AmyCoat 10 m Temperature: 25 C Sample size: 10 g Sample size: 1 mg
N.B. The relative back-pressure increase is a measure of the degree of Column size: 4.6 x 250 mm (* initial bed pressure) Detection: UV 223 nm Detection: UV 350 nm
50 250 mm column:

00 particles right from the start. degradation of the material after repeated packings. Actual particle size for 0
0
Chiralcel and Chiralpak is about 3 times larger than that for Kromasil, which 00 = 1.2
explains the difference in initial back pressure (back-pressure is inversely
0
00 proportional to the square of the particle size). 0
00

Easy to Scale up 0
The test was designed to exert greater than normal 00 0
00

Binaphtol
Easy to scale up mechanical stress on the chiral stationary phases, 0
Conditions: Conditions: Conditions: Conditions:
0 5 10 15 20 25
0
5 10 15 20 25
0.1 g
00 and is performed at a packing pressure above the Column size: 4.6 150 mm
Flow rate: 0.5 ml/min
DAC system: NovaSep Pack-n-Sep, 50 mm i.d.
Bed length: 135 mm
Column size: 4.6 150
Flow rate: 0.5 ml/min
DAC system: NovaSep Pack-n-Sep, 50 mm i.d.
Bed length: 132 mm [min.] 0 [min.]
Flow rate: 60 ml/min Flow rate: 60 ml/min Enantiomer 1 Enantiomer 2
maximum 50 bar recommended by the manufac- 500 200 0 500 000 0
Purity [%] 95.4 97.8
Yield [%] 91.7 72.8
0 = 1.4 = 1.3 = 2.9 = 3.0
turer of Chiralcel OD and Chiralpak AD. 00 Rs= 2.6
00
5 0 Rs= 2.4 00 Rs= 9.9 805 0 Rs= 7.9
0 5 10 15 0
50
80 0
50 60
[min.] 600 0 0 0
Analytical injection Preparative injection

Mobile phase: Heptane/2-Propanol/DEA (90/10/0.1)


00 00 40
Fully back-integrated 0 40 0
Kromasil Kromasil 5-CelluCoat Kromasil 10-CelluCoat
Sample size: 10 g Sample size: 20 mg
3-CelluCoat 5 5 Detection: UV 223 nm Detection: UV 290 nm
50 20 50 20
= 2.4 R = 13.9 = 2.4 R = 11.4 = 2.4 R = 10.0 00
0
s s s
We manufacture the super wide pore silica for 00
0 0 0 0 = 2.4
Common conditions Kromasil polysaccharide products and perform all 0 5 10 15 20 0 5 10 15 20 0 5 10 15 20 25 30 35 0 5 10 15 20 25 30 35 00 00
Column size: 4.6 150 mm Temperature: 25 C [min.] [min.] [min.] [min.]
Mobile phase: Heptane/2-Propanol (90/10) Solute: trans-Stilbene oxide
Flow rate: 0.5 ml/min Detection: UV 229 nm subsequent steps leading to the final product. 00
Common conditions Common conditions
Stationary phase: Kromasil AmyCoat, 10 m Detection: UV 254 nm 00
This means you get a product with full traceability. Mobile phase: Heptane/2-Propanol (90/10) Temperature: 20 C
Stationary phase: Kromasil CelluCoat, 10 m
Mobile phase: Heptane/2-Propanol (90/10)
Detection: UV 254 nm
Temperature: 20 C 00
Solute: Trifluoro-anthrylethanol Solute: Trifluoro-anthrylethanol
Mechanically strong Every manufacturing step is ensured through 0 0
2.4 g

Metoprolol
0 5 10 15 20 0 5 10 15 20
Mechanical strength is an important product our detailed quality system, and the final product [min.] [min.]
Enantiomer 1 Enantiomer 2
lifetime parameter. Kromasil AmyCoat and is never released until it has passed our rigorous Purity [%] 100 99.7
Yield [%] 97.9 88.1

Kromasil CelluCoat have a mechanically strong quality control tests. Having all the manufacturing
Common conditions
spherical silica which withstands repeated cycles steps in-house also gives us a strong position in Stationary phase: Kromasil CelluCoat, 10 m Temperature: 25 C
Column size: 4.6 x 250 mm Flow rate: 1 ml/min

of packing. terms of production capacity.


Taking a closer look.

Kromasil AmyCoat Kromasil CelluCoat


Chiral selector Before testing conditions not mentioned, Chiral selector Before testing conditions not mentioned,
The coated selector is tris-(3,5-dimethylphenyl) please contact the Kromasil application group, The coated selector is tris-(3,5-dimethylphenyl) please contact the Kromasil application group,
carbamoyl amylose. kromasil@akzonobel.com carbamoyl cellulose. kromasil@akzonobel.com

Product assortment* Product assortment*


HN

HN
3 m - AmyCoat 5 m - AmyCoat 10 m - AmyCoat 3 m - CelluCoat 5 m - CelluCoat 10 m - CelluCoat
NH O O
O
O
O 4.6 Kromasil 3-AmyCoat Kromasil 5-AmyCoat ___
HN
O
4.6 Kromasil 3-CelluCoat Kromasil 5-CelluCoat ___
O HN
x 4.6 x 50 mm 4.6 x 50 mm O O x 4.6 x 50 mm 4.6 x 50 mm
50 mm O 50 mm
O
O * O
O O *
* O O
O O O 4.6 Kromasil 3-AmyCoat Kromasil 5-AmyCoat Kromasil 10-AmyCoat O 4.6 Kromasil 3-CelluCoat Kromasil 5-CelluCoat Kromasil 10-CelluCoat
O O x 4.6 x 150 mm 4.6 x 150 mm 4.6 x 150 mm NH
HN n
x 4.6 x 150 mm 4.6 x 150 mm 4.6 x 150 mm
NH HN O 150 mm O 150 mm
O O
O O
O HN
4.6 Kromasil 3-AmyCoat Kromasil 5-AmyCoat Kromasil 10-AmyCoat 4.6 Kromasil 3-CelluCoat Kromasil 5-CelluCoat Kromasil 10-CelluCoat
NH HN O O
x 4.6 x 250 mm 4.6 x 250 mm 4.6 x 250 mm x 4.6 x 250 mm 4.6 x 250 mm 4.6 x 250 mm
*
250 mm 250 mm
n
10 ___ Kromasil-5-AmyCoat Kromasil-10-AmyCoat 10 ___ Kromasil-5-CelluCoat Kromasil-10-CelluCoat
x 10 x 250 mm 10 x 250 mm x 10 x 250 mm 10 x 250 mm
250 mm 250 mm
tris-(3,5-dimethylphenyl)carbamoyl amylose 21.2 Kromasil 5-AmyCoat Kromasil 10-AmyCoat tris-(3,5-dimethylphenyl)carbamoyl cellulose 21.2 Kromasil 5-CelluCoat Kromasil 10-CelluCoat
x ___ 21.2 x 250 mm 21.2 x 250 mm x ___ 21.2 x 250 mm 21.2 x 250 mm
250 mm 250 mm
Particle sizes Particle sizes
* Other column dimensions available upon request. * Other column dimensions available upon request.

Product codes Product codes


Kromasil X-AmyCoat followed by column diameter Kromasil X-CelluCoat followed by column diameter
and length. X indicates particle size: 3, 5 or 10 m. and length. X indicates particle size: 3, 5 or 10 m.
For example: Kromasil 3-AmyCoat 4.6 x 150 mm For example: Kromasil 3-CelluCoat 4.6 x 150 mm

Kromasil AmyCoat is available in 3 and 5 m as Kromasil CelluCoat is available in 3 and 5 m as


pre-packed columns, and in 10 m as pre-packed pre-packed columns, and in 10 m as pre-packed
column and bulk material. column and bulk material.

Compatible mobile phases Compatible mobile phases


0 10 20 30 40 50 60 70 80 90 100 % 0 10 20 30 40 50 60 70 80 90 100 %
2-Propanol in Alkane 2-Propanol in Alkane

Ethanol in Alkane Ethanol in Alkane

Methanol in Alkane* Methanol in Alkane*

2-Propanol in Acetonitrile MTBE in Alkane

Methanol in Ethanol MTBE in Ethanol

Methanol in Acetonitrile Methanol in Acetonitrile

Methanol in CO2 (SFC) Methanol in CO2 (SFC)

*Due to limited miscibility of methanol in alkanes, ethanol should *Due to limited miscibility of methanol in alkanes, ethanol should
be added as a mediator when exceeding 5% methanol. be added as a mediator when exceeding 5% methanol.

Compatible conditions Further investigations required Compatible conditions Further investigations required