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ABSTRACT Adult rats were fed isonitrogenous diets (5 % protein equivalent) providing
indispensable amino acids either in the whole-egg protein pattern or in the adult rat maintenance
pattern of Smith and Johnson (S & J, Brit. J. Nutr. 21 : 17, 1967). In two separate experiments
the threonine and the methionine (total sulfur amino acid) requirements were assessed. Similar
responses were obtained with rats on the threonine experiment independent of the pattern of
indispensable amino acids fed. In the methionine experiment the rats fed the S & J pattern
Despite several studies concerned with (7), who used a lower level of total dietary
the maintenance requirement for indispen- nitrogen and a different starting pattern of
sable amino acids of adult rats (1-3), uncer- indispensable amino acids.
tainty remains as to a reliable set of values Young et al (8) have also drawn
. atten-
that might be used in formulating adequate tion to reported differences among re-
purified diets for this species. The Subcom- searchers in requirements for indispensable
mittee on Laboratory Animal Nutrition of amino acids. They concluded that the cx-
the National Research Council (4), selected tent to which the pattern and absolute in-
the results of Benditt et al. (1) for its re- take of the various essential amino acids
quirement recommendations This is sur-
. may be modified without affecting the mini-
prising, since single animals only were used mum needs for a specific essential amino
to study any one level of a given amino acid, acid is unknown . Studies designed to estab-
and the amino acids used were with few
, lish the minimum requirement for an essen-
exceptions, composed of racemic mixtures tial amino acid given various patterns
of the indispensable amino acids Of course,
. and levels of the essential amino acids are
no statistical evaluation of the results could required to resolve this uncertainty(8)
be performed. Based on parenteral feeding While it is possible that pattern and levels
studies with adult rats, Sitren and Fisher (5) of amino acids may, indeed, be responsible
have recently suggested that the Benditt et
From the Departments of Nutrition and Home
al. values, as given by the National Re-
Economics, Rutgers University, New Brunswick, New
search Council, may be excessively high for Jersey 08903
at least four indispensable amino acids. In 2 Paper of the Journal Series. New Jersey Agricul-
studies with adult human subjects, there is tural Experiment Station.
also need to reconcile divergent published 3 This study was part of Regional Research Project
NE-73, Nutritional Improvement in the Northeast Re-
amino acid requirement values. Rose Lev- ,
gion, and was supported in part by regional research
erton and others (6) have generally reported funds.
much higher requirements than Fisher et al. 4 Professor.
932 The American Journal ofClinicoi Nutrition 30: JUNE 1977, pp. 932-938. Printed in U.S.A.
AMINO ACID REQUIREMENTS OF DIFFERENT DIETARY PATTERNS 933
for the reported differences in requirement order to more nearly equalize the nitrogen intake of the
two groups of animals, the concentration
of the S & J
for specific amino acids of adult animals and amino acid mixture was increased by 25 % (Table 1,
man, the possibility cannot be overlooked, column 2 of amino acid mixtures); this had the desired
as Hegsted recently emphasized, that effect of increasing food intake to the level of the
much of the data obtained by bal- animals on the amino acid mixture simulating the egg
pattern (Table 1).
ance studies must be artifactual for one rea-
The composition of the basal diet together with that
son or another (9). Hegsted proceeded to of the amino acid mixtures is given in Table 1 . In
point out that in his view there is no satisfac- experiment 1 L-threonine was the variable dietary con-
tory means to avoid some or most of the stituent and in experiment 2 L-methionine was the
difficulties inherent in nitrogen balance variable and served as the only source of sulfur amino
acids (cystine was omitted). Isonitrogenous additions
studies, and he warned that until new meth-
or substitutions for the variable amino acid were made
ods are available to measure nutritional re- with glycine.
quirements, the results obtained with bal- The study with college women followed the same
ance techniques must be viewed with cau- protocol as described in detail in earlier papers on the
tion and skepticism. reassessment of amino acid requirements of young
women receiving low nitrogen diets (7, 10, 1 1). As in
The present studies were carried out in an the rat studies, either of two diets were given that
attempt to elucidate the relationship, if any, provided the same amount of nitrogen per day (7 g).
of dietary amino acid pattern on the require-
(7, 11).
E
C.,
Methods a. +1
c-.ti
4
In the rat studies food consumption was measured PS
I
F-.
+1 +1 +1
dietary threonine , irrespective of the amino a. o 0
acid pattern into which the threonine had C., C.,,
retention
els of threonine
peared
response
both diets and in response
to meet
of the rats was similar on
to increasing
The level of 0 1 0% ap-
the requirement
.
1ev-
for this
.0
I E
a
V a. +1 +1 +1
amino acid. Quantitatively, nitrogen reten- 4 N
c-c-c-
fl
Cl,
tion was higher on the highest intake of
threonine (0 .1 0%) for the rats on the egg H
pattern; however, the higher level of methi- tern; therefore the concentration
, of all
omne (0 .225 %) afforded significantly bet- amino acids was increased by 25 % This .
ter nitrogen retention (Table 3). Body change successfully brought food consump-
weight changes on the egg pattern were tion and nitrogen retention for the S & J rats
small and non-significant; food consumption into line with those getting the egg pattern
remained relatively constant but was higher (compare lines 1 and 2 Table 4). Although,
in this experiment than in the previous one, the nitrogen retention results with a dietary
undoubtedly due to the changed food access methionine level of 0.07% seem out of line,
time (twice for 1 hr each versus once for 2 a break in the response pattern is clearly
hr). When the rats that had received the egg evident at 0.10 to 0.12% methionine. If the
pattern were switched to the S & J pattern good nitrogen retention with only 0.07%
(Table 3 last 2 lines), nitrogen
, retention on methionine is also considered, then it ap-
the reduced methionine intake (0.10%) was pears that for methiomne the requirement is
as good as that previously observed with the lower on the S & J pattern between one
higher level of 0.1 5 % on the egg pattern. third and one half in comparison to that
As mentioned in the experimental sec- noted on the egg pattern It is interesting
. to
tion, food intake was much lower with the S note that the rats that had previously been
& J pattern (Table 4) than for the egg pat- fed the egg pattern when switched to the S
Body Weight
L,-methionine Food consumption N retention
Start End
% ofdiet g giday mg/day
TABLE 4
Body weight changes, food consumption, and nitrogen retention of adult male
rats fed the S & J maintenance pattern of indispensible amino acids
with varying levels of L-methiomne
Body weight
UMethionine Food consumption N retention
Start End
ap diet provided amino acids according to the S & J (3) pattern as given in column 1 , Table 1 ; in all other
diets the amino acid levels were increased by 25% as given in column 2, Table 1 . iS Mean SE for eight rats;
the same animals were consecutively given the indicated methionine levels for periods of 2 to 3 weeks; collections
were made daily from Tuesday through Friday of each week; only values within a column that do not share the
same superscript(s) are significantly different (P < 0.05).
936 FISHER ET AL.
tern.
In the human lysine study (Table 5) no I
differences
with either
in body weight
of the two amino
were detected
acid patterns I. a
used. With the egg pattern a statistically a.
significant break in nitrogen retention oc-
curred at a lysine intake of 50 mg/day: re-
tention decreased significantly in reducing
the intake from the high level provided by E
egg protein (2 140 mg) and increased signifi- a.
cantly when 300 mg of lysine were pro- C
B
vided . No difference was observed in the
a.
quantitative nitrogen retention response be- C
Discussion
a
It is interesting to compare the present Lu
In contrast to our observations and those large intakes. We have already pointed out
of Said and Hegsted (14), the requirements that the direction of the change in the con-
reported by Smith and Johnson (3) were centration of an indispensable amino acid
considerably higher for both threonine may also influence the response This is par-
.
ence also prevailed during the threeonine use of plasma amino acid changes and of
study where no difference in requirement C402 monitoring after C4 amino acid ad-
for this amino acid was noted. ministration in conjunction with graded 1ev-
The lysine requirement for women has els of the amino acid under study (8 18) ,
been reported to be much higher (500 mg/ may deserve more consideration in this re-
day (16, 17)) than the 50 mg/day observed spect.
in our earlier studies (10) and again in the
present experiment. Perhaps this difference The authors express their appreciation to Mrs.
Olga Donis, Mrs. Anna Sekowski, Miss Elaine Grimm-
is explainable by an effective conservation
ger and Miss Eileen Liddy for excellent technical assist-
of lysine when the dietary supply is low or ance.
inadequate and further
, compounded by the
short experimental diet periods. However,
References
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from the present study. Alternatively, since AND L. E. FRAZIER. Studies in amino acid utiliza-
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3. SMim, E. B., AND B. C. JOHNSON. Studies of
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(10). Nutr. 21: 17, 1967.
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938 FISHER ET AL.
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