Buffalo (or buffaloe) primarily refers to:

African buffalo or Cape Buffalo (Syncerus caffer)

Bubalus, a genus of bovines including various water buffalo species
Wild water buffalo (Bubalus arnee)
Water buffalo (Bubalus bubalis)
Italian buffalo or Italian Mediterranean buffalo, a breed of water buffalo
Anoa
Tamaraw (Bubalus mindorensis)
Bison
American bison (Bison bison), also commonly referred to as the American buffalo or
simply "buffalo" in North America
Buffalo, New York
Buffalo wing, a style of chicken wing prepared with a spicy sauce coating, originally created
in Buffalo, New York
Buffalo (or buffaloe) may also refer to: