CHEMISTRY TECHNICAL MANUAL PROCEDURE

1. Received the test request from the cashier after payment.

2. The Medical Technologist confirm the name and age of
the patient, check the spelling by asking the patient.
3. Properly label the test tubes to be used of the name of the
patient according to the test requested.
4. Inform the patient ready of the procedure and select the
proper site for blood extraction, disinfect the site with
cotton wet with 70% alcohol prior to the procedure.
5. Extract blood from the patient considering the amount of
sample according to the patients requests.
6. Prepare the sample ready for examination. Let it stand to
clot for serum sample and for the tube with anticoagulant
do the mixture by tilting the tube few times to prevent
clotting of the sample.
7. Centrifuge the blood after it clot and separate the serum
from blood to the other clean test tube with properly
labelled by the name of the patient.
8. Prepare the blood chemistry reagents, take it out from the
refrigerator and allow it to cool at room temperature.
9. Switch on the Chemistry machine MapLab TC 84 Plus and
let it run the calibration and blanking until it beep and
ready to run the test.
10. The Medical Technologist should perform the
Chemistry Quality Control using pathologic and non-
pathologic sample every first week of the month of all the
chemistry test procedures to ensure the accuracy of the
test results.
11. The results in every test are recorded, get their
average and Standard Deviation (SD) and plot each
results in the graph.
12. In Chemistry machine select the number of test
according to the patients request.
13. Using micropipettes, measure the reagents and
sample.
Example: The request is test for Fasting Blood Sugar (FBS)
FBS Test Procedure:
1. Measure 1000ul of Glucose reagent and put it in the
cuvette tube. Prepare blank solution for the test.
2. Select the Glucose test number in the machine and follow
the instruction of the machine.
3. After setting the test number, the machine will ask to
insert the blank reagent. Put the blank reagent into the
cuvette and insert into the flow cell inside and press read.
4. The machine will then ask to insert the sample after the
blank.
5. Add 10ul sample to the 1000ul glucose reagent in the
cuvette tube and put it in the incubator on the machine
and incubate it for 5 minutes.
6. After 5 minutes incubation, the cuvette tube will be
inserted into the flow cell of the machine and press read.
7. The machine will display the result of the reading in the
monitor and in the print out.
8. Take note the result of the test and write it down and
record it in the logbook.
9. Please refer the other chemistry tests procedure below.
10.Encode the results and double check the print out prior
to signature of the Medical Technologist and the Pathologist
signature and put it in the releasing box ready for release.
11. The time duration of each chemistry test is 15-20
minutes including the 5 minutes incubation. Therefore the
Medical Technologist can do 15-25 chemistry tests in 8
hours shift.
12. After the test save the specimen in the refrigerator for 24
hours before disposal for any followup test request.

CHEMISTRY STEP BY STEP TEST PROCEDURES:

MAPLAB TC 84 PLUS CHEMISTRY MACHINE USED:
TEST # 8 GLUCOSE
1 ml Glucose reagent, add 10ul serum sample. Incubate for 5
minutes at 37 degree celcius, read test against reagent
blank.
TEST # 2 TOTAL CHOLESTEROL
1ml cholesterol reagent, add 10ul serum sample. Incubate
for 5 minutes at 37 degree celcius, read test against reagent
blank.
TEST # 3 BLOOD URIC ACID
1 ml Uric acid reagent, add 25ul serum sample. Incubate for
5 minutes at 37 degree Celsius, read test against reagent
blank.
TEST # 4 TRIGLYCERIDE
1ml Triglyceride reagent, add 10ul serum sample. Incubate
for 5 minutes at 37 degree celcius, read test against reagent
blank.
TEST # 5 CREATININE
500ul picric acid and 500ul NaOH mix. Add 100ul serum
sample and read right away.
TEST # 6 SGPT
Reagent preparation: 4 ml of reagent B to the bottle of
reagent A
Test: 1 ml SGPT reagent, add 100ul serum sample and read.

TEST #7 BLOOD UREA NITROGEN

Reagent preparation: 4ml of reagent B to the bottle of
reagent B
Test: 1ml BUN reagent, add 10ul serum sample and Read.