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Preparation of reagents
1.2. Heparin
2 mg heparin was dissolved in 0.9 % NaCl (10 ml). 200 l of heparin was
added to each 1 ml of blood to prevent coagulation.
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Preparation of reagents
1.4.1. Tris HCl buffer (50 mM) containing EDTA (1 mM; pH 8.2)
605 mg of Tris HCl was dissolved in 100 ml of distilled water. To this, 0.0372
g of EDTA was added and pH was adjusted to 8.2.
1.5. Catalase
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Preparation of reagents
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Preparation of reagents
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Preparation of reagents
1.11. Hexokinase
1.12. Phosphofructokinase
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Preparation of reagents
605 mg of Tris HCl was dissolved in 100 ml of distilled water and the pH was
adjusted to 8.
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Preparation of reagents
1.13.5. Glycogen (1 %)
50 mg of glycogen was dissolved in 5 ml with distilled water.
1.15. Glucose-6-Phosphatase
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Preparation of reagents
1.17. Immunoblotting
1.17.2. Ponceau S
500 mg of Ponceaus S, 7.5 g of TCA and 7.5 g of sulfosalicylic acid were
dissolved in and made up to 25 ml with distilled water.
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Preparation of reagents
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