OASIS LABORATORIES PRIVATE LIMITED, DEHRADUN

SOP No.:
QUALITY CONTROL DEPARTMENT Page 1 of 4
QCG006
Supersedes : Effective:
STANDARD OPERATING PROCEDURE
00 April 2010
Version No. : SOP FOR OPERATION AND CALIBRATION OF Review date:
01 pH METER March 2013

1.0 OBJECTIVE:

To describe the procedure for the Operation and Calibration of pH

Meter.

2.0 SCOPE:

This SOP is applicable to pH Meter at M/s. Oasis Laboratories Private

Limited, Dehradun.

3.0 RESPONSIBILITY:

Quality Control Department

4.0 ACCOUNTABILITY:

HOD QC

5.0 PROCEDURE :

5.1 Ensure that the instrument is clean and free from dust.
5.2 Ensure the plug is connected to the mains.
5.3 Press power ON / OFF switch to ON position.
5.4 Set the temperature compensation knob to the temperature of
solution.

5.5 Place electrodes in buffer solution of pH 7.0.

5.6 Adjust the potential knob to pH position.
5.7 Adjust the pH of the buffer solution to pH 7.0 with the help of
standardize knob.
5.8 Remove the electrode form buffer 7.0 wash, and place then in
buffer 4.0.
5.9 Adjust the pH 4.0 with the help of the slop control knob.
5.10 Remove the electrode form buffer 4.0 wash, and place then in
buffer 9.2.
5.11 Adjust the pH 9.2 with the help of the control knob.

Designation Signature Date

Prepared by Chemist - QC
Reviewed by Manager - QC
Approved by Manager - QA
 OASIS LABORATORIES PRIVATE LIMITED, DEHRADUN
SOP No.:
QUALITY CONTROL DEPARTMENT Page 2 of 4
QCG006
Supersedes : Effective:
STANDARD OPERATING PROCEDURE
00 April 2010
Version No. : SOP FOR OPERATION AND CALIBRATION OF Review date:
01 pH METER March 2013

5.12 Remove the electrode and wash, repeat the process by dip the
electrode in the buffer having pH 7.0, 4.0 and 9.2 till the
observed value get correctly.

5.13 Rinse the electrode with distill water and place it in sample and
read the pH of the sample.

5.14 After adjust the potential knob at check position switch of the
instrument when not in use for long time.

6.0 CALIBRATION:-
To achieve accuracy the instrument is calibrated by the following
procedure:-
The pH meter is calibrated daily with buffer solution having pH 4.0, 7.0
and 9.2 as per the operating procedure.

7.0 PRECAUTION:-
7.1 Do not leave electrode dry, when not in use, keep the electrode in
distilled water.

7.2 Do not wipe the glass electrode bulb with any coarse material.
7.3 The buffer should not get contaminated.
8.0 RECORDS:

Calibration Record of pH Meter.(annexure-1).

9.0 ANNEXURES:
Annexure-1 & 2 Format for Calibration Record of pH Meter.

10. ABBREVIATIONS & DEFINITIONS:

SOP : Standard Operating Procedure.
Dept. : Department.
HOD : Head of the Department.
QC : Quality Control
Annexure-2
pH BUFFER PREPARATION RECORD

SOP REF NO QCG006 QC-F-024-00

Designation Signature Date
Prepared by Chemist - QC
Reviewed by Manager - QC
Approved by Manager - QA
 OASIS LABORATORIES PRIVATE LIMITED, DEHRADUN
SOP No.:
QUALITY CONTROL DEPARTMENT Page 3 of 4
QCG006
Supersedes : Effective:
STANDARD OPERATING PROCEDURE
00 April 2010
Version No. : SOP FOR OPERATION AND CALIBRATION OF Review date:
01 pH METER March 2013

Location: Q.C. Lab.

Date Buffer solution Make Qty. Qty. Next Due Remark Prepared Checked
Received Balance Date by by
Buffer pH 4.0

Buffer pH 7.0

Buffer pH 9.2

Annexure-1
pH BUFFER PREPARATION RECORD

SOP REF NO. QCG006 QC-F-007-00

Location: Liquid Mfg. Section

Date Buffer solution Make Qty. Received Qty. Next Due Remark Prepared Checked
Balance Date By by

Designation Signature Date

Prepared by Chemist - QC
Reviewed by Manager - QC
Approved by Manager - QA
 OASIS LABORATORIES PRIVATE LIMITED, DEHRADUN
SOP No.:
QUALITY CONTROL DEPARTMENT Page 4 of 4
QCG006
Supersedes : Effective:
STANDARD OPERATING PROCEDURE
00 April 2010
Version No. : SOP FOR OPERATION AND CALIBRATION OF Review date:
01 pH METER March 2013

Buffer pH 4.0

Buffer pH 7.0

Buffer pH 9.2

Designation Signature Date

Prepared by Chemist - QC
Reviewed by Manager - QC
Approved by Manager - QA