OM-505

DNA and RNA from saliva

formicrobiology sampling

All-in-one system for the collection and rapid stabilization

ofmicrobial nucleic acids
Microarrays and Next Generation Sequencing give researchers the ability to easily detect and
quantitate molecular targets in ahighly multiplexed fashion. Due to the complex nature of
samples required for metagenomic research of the microbiome, bias canbeeasilyintroduced
through improper sample collection, stabilization and transport.

Provides a snapshot of the microbial profile at time ofcollection

and prevents microbial growth
Stabilizes RNA at room temperature and DNA
upto37C for 3 weeks
Easy self-collection of high quality DNA and
RNA from oral samples
Eliminates the need for cold chain transportation
Compatible with downstream applications
Sample ready for
(e.g.,RTqPCR,microarray) transport, storage
andprocessing

By using HOMIM (Human Oral Microbe Identification Microarray), we demonstrated

thatsaliva samples stored in OMNIgene were stabilized for at least 3 weeks, even at
Collection device catalog #: OM-505
37degrees. This is outstanding! Consequently, I think OMNIgene would alleviate the
needfora -80 freezer or, for that matter, any refrigeration to preserve samples. This is an For more information contact
incredible benefit for any investigator who is interested in preserving precious clinical samples, info@dnagenotek.com
especially for use with molecular analyses of genomic material.

Bruce J. Paster, Ph.D., Director, Human Microbe Identification Microarray Core

Head, Department of Molecular Genetics, The Forsyth Institute

For Research Use Only DNA Genotek Inc. Toll-free (North America): 1.866.813.6354
Not for use in diagnostic procedures 2 Beaverbrook Road Tel.: 613.723.5757 Fax: 613.723.5057
Ottawa, ON, Canada K2K 1L1 www.dnagenotek.com
Subsidiary of OraSure Technologies, Inc. info@dnagenotek.com
www.dnagenotek.com
Benefits Easy collection
Stabilizes samples at point of collection ensuring the DNA and RNA is unchanged at time
ofprocessing
Facilitates simultaneous detection of DNA and RNA from bacteria and viruses all from
onesample
Reduces transportation and storage cost and complexity with 3 weeks of ambient temperature
stability of DNA and RNA
Fill line
Enables identification of the living or metabolically active bacteria at time of collection
throughRNA expression profiling Spit until the amount
ofsaliva (not bubbles)
Format compatible with high-throughput processing increases efficiency and minimizes reachesthefill line.
samplehandling errors

Supporting evidence
Subject Target Reference
Virus DNA EBV1, HHV-82, 3, CMV4
RNA HCV5, HIV5, 6
Bacteria DNA HOMIM7 Close lid tightly by pushing
down hard onthefunnel lid
RNA E. coli9 until youhear a loud click.
Extraction systems Abbott m20005, 6, Qiagen MinElute8
Downstream applications qPCR1, 2, 3, Microarray7
1 Stability, recovery efficiency and detection limit of human viral DNA inOMNIgeneDISCOVER (OM-505). DNA Genotek. PD-WP-00028.
2 High quality microbial DNA collected with OMNIgeneDISCOVER enables detection of HHV-8 in saliva. DNA Genotek. MK-00018.
3 Evaluation of a new commercial kit for detection of human herpesvirus 8 (HHV-8) in saliva. http://www.dnagenotek.com/ROW/pdf/GriffithUniversity.pdf
4 Genotyping of cytomegalovirus from toddlers saliva samples collected with OMNIgeneDISCOVER. DNA Genotek. MK-00050.
5 Compatibility of saliva collected using OMNIgeneDISCOVER (OM-505) with the Abbott m2000rt RealTime System for the detection of viral RNA.
DNAGenotek.PDWP-00025.
6 OMNIgeneDISCOVER (OM-505) stabilizes RNA from human viruses at room temperature for several weeks. DNA Genotek. PD-WP-00024.
Unscrew the funnel
fromthetube.
7 OMNIgeneDISCOVER stabilizes microbial DNA profiles in oral fluid samples, enables more precise characterization of oral flora. DNA Genotek. MK-00090.
8 OM-505 Microbial DNA and RNA purification protocol using QIAGEN QIAamp MinElute Virus Spin Kit. DNA Genotek. PD-PR-00214.
9 Internal validation using OM-505 extraction8 in house RT-qPCR.

OM-505 kit contents

Product specifications
Pre-use with packaging:
Dimensions: 14.0 x 8.0 x 2.8 cm Close tube tightly
Weight: 39 g withsmallcap.
Shelf-life: 24 months
Post sample collection specifications
Collector Standard false bottom tube:
Tube diameter: 16 mm
Packaging Tube height (without cap): 93 mm
Sample stability: 3 weeks
Collected specimen is potentially infectious
andshould behandled withappropriate Shakefor 10seconds.
Tube cap biosafetypractices.

Ship in accordance to applicable regulations

Instructions EuropeanPatent No. 1956 969;
covering transportof biological specimens. Patent pending
Canadian Design Nos.
127470;132896; 132897
OMNIgeneDISCOVER is for research use only, not for use in diagnostic procedures. U.S. D631,554 S and D640,795 S
Some DNA Genotek products may not be available in all geographic regions. Community Design Nos.
OMNIgene is a registered trademark of DNA Genotek Inc. All other brands and names contained herein are the property of their respective owners. 001095186-0001; -0002; -0003

Superior samples Proven performance

For Research Use Only PD-BR-00057 Issue 2/2012-06
Not for use in diagnostic procedures 2012 DNA Genotek Inc., a subsidiary of OraSure Technologies, Inc., all rights reserved. www.dnagenotek.com