Molecular innovation that actively

prevents aging
The first Anti-RCS Protects DNA from Detoxifies
active in cosmetics UVA-induced damage cutaneous cells

Description Properties
GHK tripeptide stabilised within the tertiary Prevention and protection of the skin from
restructure structure of the vegetal protein, is able to extrinsic and intrinsic agents which cause
detoxify the skin from the noxius Reactive premature aging.
Carbonyl Species (RCS) and protects the skin
from photoaging. Applications

anti-radical aldenine can be incorporated in daily cosmetic
Appearance formulations where prevention of photoaging
Translucent solution containing 0.1% Tripeptide-1 is desired, improving simultaneously the
(GHK). elasticity and firmness of the skin. Also suitable
for sun care products.
INCI
Water (Aqua), Hydrolyzed Wheat Protein, Dosage
Hydrolyzed Soy Protein, Xanthan Gum, 2-5 %
Tripeptide-1.
Please contact us for information on the preservative system. Solubility
Water soluble.
Science

aldenine is the first cosmetic active ingredient
that is able to capture noxius RCS, avoiding the
necrosis of the keratinocytes and the loss of
collagen elasticity. At the same time it protects
the skin from DNA damage induced by UV
radiation.

is a registered trademark of Lipotec S.A. We research for you www.lipotec.com

In vitro efficacy
Relative capture of RCS RCS are captured efficiently
Evaluation of the quenching activity of GHK versus 4-Hydroxynonenal (HNE) by GHK
and Acrolein (ACR) GHK forms adducts with HNE,
Comparative quenching activity
100
the cells are detoxified and
remain healthy.

(%) Quenched aldehyde

80
GHK (1mM)vs ACR (30uM) 60
GHK (1mM)vs HNE (30uM) 40
20

0
0 1 2
Time (h)

The quenching activity of GHK is dose-dependent.

Glycation inhibitory activity of GHK Glycation inhibitory effect

Study of the inactivation of Superoxide Dismutase (SOD) Avoids the formation of Advanced
use dosage Glycation End-products (AGEs), which
SOD Relative activity (%)

100
are effective indicators of protein
80
damage.
60
40
20

0
control 10 1 0.1 0.01
GHK (mg/ml)

The inactivation of SOD by its reaction with fructose is used as a model of glycation.

Synthesis of Collagen III 300% Increase of Collagen III

An ELISA test with monoclonal antobodies was tested in Human Dermal synthesis
Fibroblasts. Collagen III is characteristic of youthful
1.000
OD 492 mm

0.800 skin, and its increase provides a healthy

0.600 skin.
0.400
0.200
- 0.000
0 1 10 100 500
Hydrolysed vegetal protein ( g/ml)

The increase in Collagen III can be seen even after 24 hours but a dose
dependent result is obtained after 7 days.

Cellular Detoxification Detoxifies cutaneous cells

Keratinocytes were irradiated with a low UVB dose (50 mJ/cm2) in
When the skin is exposed to the
order to evaluate the quenching activity of GHK. sunlight, the cells need GHK to
quench HNE and other noxious RCS.

HNE 200 M + UVB HNE 200 M + GHK 20mM + UVB

Keratinocytes detoxify endogenously-formed HNE by forming adducts with GSH

(glutathione), the skins natural antioxidant. However, when submitted to UVB,
keratinocytes are depleted of GSH, they can no longer detoxify from HNE and
they die. 97% DNA Protection
DNA Protection Protects skin cells from UVA-
The DNA protection was tested in primary cultures of human melanocytes induced DNA damage.
using the alkaline comet assay.

Uva treated cell (positive control) Cells + UVA + 2%

showed to have an internal photoprotection capacity against UVA light.

While the claims and supporting data provided in this publication are believed to be reliable and they are presented free and for guidance only, there are
no warranties of any kind. All express and implied warranties are disclaimed. The recipient is solely responsible for ensuring that products marketed to
consumers comply with all relevant laws and regulation. LIPOTEC is the exclusive holder of the both industrial and intellectual property rights identified
herein. Recipient of this publication agrees to indemnify and hold harmless each entity of the LIPOTEC organization for any and all regulatory action arising
from recipients use of any claims or information in this publication, including, but not limited to, use in advertising and finished product label claims, and
not present this publication as evidence of finished product claim substantiation to any regulatory authority.

Isaac Peral 17. Pol. Industrial Cam Ral - 08850 Gav (Barcelona) Spain - Tel +34 93 638 80 00 / Fax +34 93 638 93 93 - commercial@lipotec.com
Ed.9B