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CLINICAL RESEARCH

Bio-Emulation: biomimetically
emulating nature utilizing a histo
anatomic approach; visual synthesis

Panaghiotis Bazos, DDS


Emulation, Athens, Greece

Pascal Magne, DMD, MSc, PhD


Associate Professor, Don and Sybil Harrington Foundation,
Chair of Esthetic Dentistry, Herman Ostrow School of Dentistry,
University of Southern California, Los Angeles, USA

Correspondence to: Panaghiotis Bazos DDS


33 Vasilissis Sophias Avenue 106 75 Athens, Greece. Tel: +30 210 722 2329; E-mail: p_bazos@mac.com

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Abstract
A thorough understanding of the spatial Themain goalsfor this article are to cog-
distribution pertaining to the histo-ana- nize histo-anatomic visualization by in-
tomic coronal structures and dynamic troducing: (1) Dynamic light interaction,
light interaction of the natural dentition (2)the 9 elements of visual synthesis,
provides the dental team with the ulti- (3)
dynamic infinite optical thickness,
mate strategic advantage with regards and (4)amplified visual perception ef-
to optical integration of the final restor- fect of the hard dental tissues. Further-
ation. The second part of this two-part more, a diversification of photographic
article will attempt to provide insight on illumination techniques will be illustrat-
the illumination interactivity and the spa- ed in order to juxtapose optical asso-
tial arrangement of the coronal elements ciations between the enamel/dentinoe-
of natural teeth through the utilization of namel complex/dentin nexus.
this knowledge in the clinical and techni-
cal restorative approach. (Int J Esthet Dent 2014;9:330352)

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DENTIN OPACIFICATION FLUO

R E L A T I V E
DEC TRANSPARENCY FLUO

ENAMEL TRANSLUCENCY OPAL

Fig 1 Relative attribution: although translucent by nature, the coronal structural elements can be graded
with regards to their relative dynamic light interactivity and unique optical expression.

Introduction luster (S/G/L) should also be appraised


in conjunction with translucency, opal-
In the modern dental practice, recreat- escence and fluorescence (T/O/F) as
ing the optical features of the intact tooth part of the process of visual assessment
presents a formidable task, due to the in- (Fig2).
herent translucent nature of enamel, the
dentinoenamel complex (DEC) and den-
tin. Translucent materials offer a signifi- Dynamic light interaction
cant color measurement challenge since
they interact with light in a far more com- Reflection and refraction
plex manner than most other materials. in enamel and dentin
While being translucent by nature,
when coronal structures, such as enam- Incident light ray interactivity with a tooth
el, the DEC and dentin, are compared can be:
among each other, they seem to pos- Reflected specularly and/or diffusely
sess relative translucency, transpar- from its surface (Fig3)
ency and opacity respectively (Fig1). Refracted and either:
Anachronistic traditional visual estima- scattered within it and subsequently
tion approaches that solely employ the reflected, a process largely respon-
Munsell color model system based on sible for color perception (Fig4)
hue, chroma, and value (H/C/V) domi- 
transmitted diffusely through it (re-
nate the dental market appear to be lating to the properties of transpar-
inadequate when conveying the perti- ency, translucency and opacity)
nent information among the dental team (Fig5)
members (clinician/technician/patient). absorbed within it (the electromag-
Further information regarding the de- netic energy is transformed to other
scription of surface texture, gloss, and forms of energy, eg, heat, photolu-

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S G L descriptive

H C V objective

T O F subjective

9 Elements

Fig 2 Dynamic light interaction will generate the visual synthesis which is influenced by nine elements:
surface texture, gloss and luster (S/G/L); hue, chroma and value (H/C/V) and translucency, opalescence
and fluorescence (T/O/F).

Incident Light Ray

Diffuse Reflection

Specular Reflection
Reflection

Fig 3 The term reflectance is used to denote the fraction of light energy that is reflected by the surface
of a given material. If the surface is not plane but curved, as is the case with enamel above, it may still be
considered to be made up of many very small, elementary plane surfaces.

DENTIN DEC ENAMEL

1.54 1.43 1.63


Refraction

Fig 4 The term refraction is used to denote a change in direction of propagation of light waves as a result
of its traveling at different speeds at different points among the wave front between mediums of varying
optical densities. Primary subsurface scattering is denoted by the radial arrow depictions.

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T
H
G
LI
T
N
E
ID
C
IN

DENTIN

T
H
G
LI
D
TE
IT
SM R
E
N F
A L
TR E
C
T
E
D
L
IG
H
T DEC

ENAMEL

Fig 5 Light interactivity model. From the cervical to the incisal regions, the dominance of the dentin core
gradually gives way to that of the enamel shell respectively, achieving a brief equilibrium in the middle
region. Multi-directional forms of scattering (colored arrows) and refractive index variations between the
enamel/dentinoenamel complex/dentin substrates create infinite photonic pathways, collectively rendering
a unique visual synthesis depending upon the incident light direction and intensity.

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minescence, photoelectric effect Light guiding by scattering


etc) in enamel and dentin
re-radiated with a lower energy state
(eg, fluorescence) The heterogenous composition and
asymmetric directional distribution of
Relative refractive index the hard dental tissue structural compo-
nents add to the level of complexity with
Due to the fact that enamel and dentin regards to microscopic light interactivity.
are heterogeneous hydrated substrates Natural waveguides,8 such as enamel
of variable inorganic and organic com- and dentin, are differentiated from con-
position, one must consider collectively ventional optical fibers by being non-
the volume fraction of their individual uniform and containing scattering parti-
elemental components refractive index cles. Nonetheless, they have the ability
(RI), resulting in their respective relative to collect light and transport it purposely
refractive index (RRI). Moreover, de- towards the pulp chamber (Fig5).9-12
pending on the localized mineral content Scattering generally implies a forced
of these substrates, minor fluctuations deviation of light from a straight trajec-
in the RI may ensue, with the highest tory by localized non-uniformities (scat-
values always occurring within the more terers), found upon or within the me-
mineralized locations.1,2 dium through which it interacts, without
The structural orientation and the ar- the loss of energy. Reflection, refraction
rangement of the enamel prisms do not and diffraction represent various forms
seem to have a significant effect on light of scattering. With regards to enamel
attenuation, resulting in an RRI value of and dentin, multiple scattering path-
1.63.3,4 Unlike enamel, the structural ori- ways are prevalent. In the quantum pic-
entation and arrangement of the dentin ture, when the wavelength (frequency)
tubules seem to play a significant role of the scattered light is the same as the
with regards to the RRI of dentin. Tra- incident light, elastic scattering occurs.
ditionally, dentin has been cited with a Conversely, when the emitted radiation
generalized RRI value of 1.54.3 Contem- has a wavelength different from that of
porary localized RRI values for dentin the incident radiation, inelastic scatter-
subadjacent to the dentinoenamel com- ing occurs.
plex (DEC) include 1.60 (cervical), 1.56 The inorganic component of the den-
(middle), and 1.49 (incisal).4 tal hard tissues is responsible for elastic
The DEC, being an organic proteina- scattering; via Rayleigh scattering (rath-
ceous continuum5 that is dominated pri- er isotropic, only depending on the po-
marily by Type I collagen, has an RRI larization of the wavelength) in the case
value of 1.43.6,7 of enamel and via Mie scattering (rather
anisotropic, forward scattering is pre-
dominant) in the case of dentin,13 while
the organic component of the dental
hard tissues is responsible for inelastic
scattering; via fluorescence.

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a b

c d

e f

Fig 6 The frontal longitudinal tooth section was submerged in distilled water and photographed on a
black background. Despite using the same amount of direct reflective illumination per exposure, a direc-
tional change of 90 degrees reveals stunning and complex light transmissive and reflective pathways,
emphasizing the optical anisotropy of dentin. Sharp details and remarkable contrast within the dentin shade
is seen (c). Pronounced backscattering across the enamel prisms and the dentin tubules render a diffuse
appearance obscuring critical details as evident in depiction (d), based on incident light direction. Paral-
lel illumination with respect to the long axis of the tooth present on the left and perpendicular on the right.

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Dentin tubule direction at the DEC

parallel 0

oblique 45

POV

perpendicular 90

Fig 7 Dentin subadjacent to the DEC exhibits a transitional orthogonal rotational orientation from cervical
to incisal with regards to the dentin tubules. Hence, despite the fact that cervical dentin is thicker, due to
the parallel orientation of of the tubules, it is rendered more translucent. The opposite happens with respect
to incisal dentin, despite the fact that it is thinner, due to the perpendicular orientation of the tubules, it is
rendered more opacious.

In the case of enamel, major random DEC, scattering is decreased due to


scattering occurs on the ultrastructur- low tubule density with small tubule size,
al level from the hydroxyapatite (HAp) compared to the dentin directly adja-
crystal subunits, whereas minor random cent to or above the pulp due to high
scattering occurs on the microstructural tubule density with large tubule size.21
level due to the prism sheaths/interpris- Thus there exists a significant translu-
matic material orientation in conjunc- cency gradient, that of superficial den-
tion with the sinuous paths of Hunter- tin which is more translucent and that of
Schreger bands.14,15 The scattering deep dentin, which is three times more
coefficient appears to increase with opacious.22 Additionally, the regional
shorter wavelengths,16 while thin enam- variation of dentin tubule orientation is of
el sections also exhibit a Fraunhofer dif- particular relevance with regards to light
fraction pattern in the plane perpendicu- transmission, rendering cervical dentin
lar to the enamel prisms,17 acting as a as highly transmissive, middle dentin
diffraction grating which in turn may be as moderate, and incisal dentin as low
responsible for generating iridescent re- (Fig7).
flections (Fig6). Conversely, the DEC lacks significant
In the case of dentin, multiple direc- scatterers. The elevated lateral light dif-
tional scattering occurs on the micro- fusion that occurs at the DEC has been
structural level due to the presence and described as the glass layer or high
spatial arrangement of the dentin tubules diffusion layer or brilliance zone.23,24
and the collagen fiber mesh.14,18,19 In Factors that may be considered for this
contrast with enamel, the scattering co- enhanced light diffusion is that the in-
efficient does not change significantly ner aprismatic enamel presents a more
with wavelength.20 Directly below the uniform HAp crystal orientation, con-

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Fig 8 Direct illumination is used to enhance the sensation of surface topography of the maxillary central
incisors. Moderate wear is viewed on the vertical developmental lobular heights of contour whereas the
concave depressions have retained parts of their original horizontal structural anatomy; diffuse light was
utilized via indirect illumination to enhance the sensation of gloss and luster.

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sequently producing an elevated light- Luster


flux density concentration at this junc- Luster describes the qualitative correla-
tion,25,26 while the underlying mantle tion of the visual appearance produced
dentin exhibits low tubule density and by the reflection of light with the enamel
small tubular size, providing minimal surface. Also known as contrast gloss,
scattering. Furthermore, the change in luster can be somewhat subjective, ex-
the RRI between dentin and enamel re- pressed in relative terms such as satin-
sults in partial reflections of light at this like, pearly, metallic, glass-like (Fig8).
junction and theoretically, when condi-
tions are favorable for short distances, Objective color elements
localized total internal reflections may
be attained. Hue
Hue Is defined as the name that distin-
guishes one family of colors from an-
The nine elements other. Hue is specified as the dominant
range of wavelengths in the visible spec-
of visual synthesis
trum that yields the perceived color.27
Descriptive surface elements The base shade of dentin primarily de-
termines the hue of a tooth.28,29 Hue can
Surface texture be considered the quality of pigment
Surface texture describes the physical (Figs9 and 10).
characteristics of the enamel surface,
being directional (vertical developmen- Chroma
tal lobes/horizontal cervical ridges) and Chroma is defined as the saturation,
structural (perikymata). Perpetual sur- intensity, or strength of the hue. Unlike
face texture adaptation is a function of value, which occurs independently of
the inherent microstructure at eruption hue, chroma is only present when there
and the subsequent physical and chem- is hue.30 Chromaticity is an objective
ical processes that modify it: attrition, specification of the quality of a color
abrasion, and erosion (Fig8). regardless of its value, that is, as de-
termined by its hue and chroma, and
Gloss is readily visualized via cross-polarized
Gloss describes the visual perception reflective photography (Figs9 and 10).
based on the interaction of light with the
physical characteristics of the enamel Value
surface, relating to the ability to reflect Value is defined as the relative white-
light in a specular (glossy appearance ness or blackness of a color and is de-
observed on polished convex con- termined by comparing it to a gray of
tours) or diffuse (matte appearance similar brightness. Value is also called
observed within concave depressions) lightness, brightness or luminance
manner. Like color, it exhibits physical, (Figs9 and 10).
physiologic, and psychological aspects
(Fig8).

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Fig 9 The intact teeth comprising the maxillary triad were extracted concurrently due to periodontal
reasons and deemed as exemplary dental specimens for exploring interdental structural and optical
inter-relationships. Aggressive acidulation led to the selective enamel dissolution and revealed the dentin
substructure. Lobular coalescence is particularly prevalent in the central incisor.

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C +

M HUE ++

I +++

C +++

M CHROMA ++

I +

C ++

M VALUE +++

I +

Fig 10 The objective color elements as viewed and estimated; general congruency is observed among
the cervical (C), middle (M) and incisal (I) thirds amongst the maxillary triad. A multitude of hues is found
at the incisal third due to the phenomena of opalescence and counter-opalescence. Chroma is more pro-
nounced at the cervical third due to the thickness of dentin.31 In all instances Value is highest at the middle
third, due to the fact that the enamel and dentin present an equilibrium in terms of thickness ratios.32

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Fig 11 The longitudinal histological tooth section of a maxillary central incisor, 1mm in thickness, was
submerged in distilled water and photographed via transmissive illumination (upper) and reflective illumina-
tion (lower) to epitomize the opalescent nature of enamel.

Subjective optical elements wide to 8m high) meet this property be-


cause the HAp subunit crystals exhibit
Translucency thicknesses of 25 to 40nm, and widths
Translucency is defined as a gradi- of 40 to 90nm. For that reason the short
ent between transparency (complete blue wavelengths reflect preferentially
transmission of light) and opacification from the enamel, while the longer am-
(complete reflection of light). The light ber wavelengths transmit accordingly
transmission of enamel has been shown through it (Fig11).
to be wavelength specific, age related
and is influenced by its state of hydra- Fluorescence
tion. A decrease in translucency during An example of photoluminescence is a
dehydration is explained as a result of phenomenon in which invisible UV light
an increased difference in refractive in- is absorbed and then re-emitted almost
dices between the enamel prisms and immediately34 (10-8 s) at a less energet-
the surrounding medium when water is ic wavelength in the visible spectrum.
replaced by air.33 Enamel and dentin both possess fluo-
rescent properties, with dentin generally
Opalescence exhibiting three times the intensity than
Known as the Rayleigh scattering ef- that of enamel35 upon longitudinal sec-
fect; enamel demonstrates this dichroic tion, emitting a white-blue luminescence
effect, which is caused by scattering after excitation, imparting additional vi-
particles with typical dimensions much tality and brightness to the natural tooth
smaller than the wavelength of illumina- appearance predominantly in UV rich
tion used. The mineral crystals present environments only. The DEC also exhib-
in the enamel prism (measuring 4m its elevated fluorescence due to the col-

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ENAMEL DENTIN
+ +++

Fig 12 A submerged maxillary premolar was photographed with reflected long wavelength UV illumina-
tion (365nm), enabling the visualization of dentin exhibiting three times the fluorescence intensity than that
of enamel. Note that the DEC also exhibits pronounced fluorescence.39-41

lagen-rich, highly cross-linked composi- of that material either on a white or black


tion, with primary intrinsic (endogenous) background, defines its infinite optical
fluorophores being the aromatic amino thickness (IOT).
acid tryptophan36,37 and the collagen Although light transmission in enamel
cross-linking agent hydroxypyridium at 1mm has been tentatively measured
(Fig12).38 to be 66% 11%, while that of dentin at
1mm is 44% 12%,47 one must con-
sider the significance of sample loca-
Dynamic infinite optical tion, the relative thickness distribution of
both tissues as well as stage of tissue
thickness
maturation.48
Perceived color can be considered a With regards to dynamic aging on
combination of the reflected color of the the macrostructural level, enamel inad-
translucent enamel layer plus the color vertently goes through a volumetric re-
reflected from the underlying relatively duction via functional wear, mechanical
opacified dentin layer. As the enamel abrasion and chemical erosion, result-
and dentin layers vary inversely in rela- ing externally in a hyperpolished sur-
tive thickness from cervical to incisal, face. Conversely, internally the dentin
the amount of color contribution from volume increases via secondary and
the two tissues will be reciprocal (vice tertiary dentin deposition.
versa) (Figs13 and 14).4446 On a microstructural level, a signifi-
The thickness of a given translucent cant reduction in the porosity of the
material at which any further material ad- enamel is due to posteruptive matura-
dition does not alter the transmission of tion via hypermineralization4951 and
light, nor the perceived reflected color homogenization leading to HAp crys-

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enamel thickness
Average facial

C 0.230.40mm 0.260.30mm 0.240.40mm 0.3mm

M 0.800.99mm 0.800.92mm 0.800.90mm 0.9mm

I 1.001.19mm 0.901.02mm 0.861.00mm 1.0mm

ENAMEL
+++ IOT

+++
translucen
cy gradien
additional t
material th
ickness (+
++) does
not alter op
tical expres
sion

Infinite Optical Thickness

Fig 13 Individual and pooled averages with regards to labial enamel thickness for the maxillary triad at
the cervical (C), middle (M) and incisal (I) thirds.44-46

tal elongation,52 while in a homologous appearing more transparent grey (lower


manner, hypermineralization of dentin value) due to less light scattering.
ensues via natural tubular obliteration, Further research is desired in order
rendering dentin more translucent over to estimate the dynamic IOT values for
time. enamel and dentin at different stages of
Due to this dynamic cycle of events, tissue maturation, thus establishing uni-
juvenile enamel, which is thicker, pos- versal industry standards for composite
sesses a texturized surface and is com- resins and etchable ceramics. Possess-
posed of small HAp crystals, appearing ing knowledge of the dynamic IOT and
to be translucent white (higher value) the degree of translucency and opacity
due to more light scattering, while adult of dental resins53,54 and etchable ce-
(and senior) enamel, which is thinner, is ramics at given thicknesses will auto-
characterized by a polished surface and matically enable clinicians and techni-
composed of large HAp crystals, thus cians to strategize accordingly in order

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DENTIN thickness
Average facial

C 2.5mm 1.8mm 2.2mm 2.2mm

M 1.9mm 1.4mm 1.4mm 1.6mm

I 0.2mm 0.2mm 0.2mm 0.2mm

DENTIN

+++
IOT

+++
translucen
cy gradien
t
additional
material th
ickness (+
++) does
not alter op
tical expres
sion

Infinite Optical Thickness

Fig 14 Pooled averages with regards to labial dentin thickness for the maxillary triad at the cervical (C),
middle (M) and incisal (I) thirds.44

to provide adequate tooth reduction to a stunning effect; an optical illusion of


meet the specific objectives that are re- magnification55 and spatial proximity is
quired. perceived with regards to the underlying
dentin mamelons. This apparent mag-
nification manifests in an incisobuccal
Amplified visual direction, creating an optical illusion with
regards to the position and dimension
perception effect
of dentin mamelons. This optical illusion
Due to the convex lens-like shape of is subdued in part by the birefringent
enamel in conjunction with possessing nature of enamel, obscuring details ren-
an RRI of 1.63, optical distortion oc- dering a hazy net appearance (Fig15).
curs. Thus, light being refracted within Concurrently, dentin also exhibits mag-
enamel, the DEC and subsequently in- nification properties56,57 due to the di-
ternally reflected from dentin produces vergent radial fanning of the dentin tu-

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apparent dentin mamelons

actual dentin mamelons

Fig 15 Enamel is responsible for creating an optical illusion of the apparent versus the actual position
with regards to the visualization of the underlying incisal dentin.

bules when light is reflected internally graphic techniques aimed at increasing


from the deeper strata. the accuracy and objectivity of dental
shade evaluation and laboratory com-
munication. In order to minimize the
Diversification of user-dependent error in future clinic-
al practice, it is necessary to develop
photographic illumination
standardized, reproducible imaging
techniques
modalities and objective image analysis
Intact dental specimens provide the ul- methods (Figs16 and 17).
timate reference for the perpetual de-
votion of time and attention to acquire Reflective Illumination
the needed knowledge with regards to
visual interpretation.58,59 Direct reflective illumination utilizing a
Over the last decade, there has been macro twin flash, via manual standardi-
profound interest in alternative photo- zation of power output, remains the pho-

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Fig 16 Facial illumination techniques from top to Fig 17 Palatal illumination techniques from top to
bottom: reflective, reflective cross-polarized, reflec- bottom: reflective, reflective cross-polarized, reflec-
tive UV and transillumination. tive UV and transillumintion.

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tographic standard for providing pre- estimation of incisal enamel distribu-


dictable and repeatable levels of light tion. Opalescence may also be visually
for shade estimation. Indirect reflective assessed and gauged via this type of
illumination (Lumiquest, Pocket Bounc- photography. To obtain such an image
ers,) on the other hand, aids in revealing intraorally, a fiber optic transilluminator
fine surface texture details. is utilized (Micro-Lux, AdDent).

Reflective cross-polarized
illumination Discussion
This photography technique significant- The restorative task is elaborated along
ly mitigates unwanted specular reflec- four levels of integration: biological,
tions which obscure the fine details of functional, mechanical, and optical.
dental structures23,60, while providing a To meet contemporary challenges, the
high contrast/hypersaturated dental im- dental team must enhance its capacity
age to be objectively analyzed via a cali- in all four levels equally.
brated RAW workflow utilizing a generic From an optical standpoint in ambi-
software program (Adobe Photoshop) ent light, enamel can be considered
in the CIE L*a*b color space. To obtain isotropic, with the visual gradient being
such a photographic image intraorally, expressed in the vertical direction (cer-
a cross-polarization filter is utilized (po- vical/incisal) due to thickness variation,
lar_eyes, Emulation). whereas dentin can be considered ani-
sotropic, with the visual gradient being
UV Illumination expressed: a) in a radial direction due
to the dentin tubule attributes of diam-
Ultraviolet Illumination is utilized in or- eter and density (qualitiative), and b) in a
der to induce fluorescence and aid in horizontal zonal direction (cervical/mid-
the selection of the restorative material dle/incisal) due to thickness variation
(etchable ceramics and resins) with a and differing RRI indexes (quantitative).
similar fluorescence intensity,61-63 pro- Hue and chroma are predominately
viding the restoration with optimal inte- determined by the properties of dentin,
gration primarily in the event of exposure dynamically changing over time as sec-
to a UV dominant lighting environment, ondary deposition occurs. In some in-
such as a dancehall or a nightclub. To stances, as in the cases of severe incisal
obtain such an image intraorally, a cus- wear, dentin can be breached to such
tom modified xenon flash tube is utilized an extent that external chromophores
(fluor_eyes, Emulation). become readily absorbed, resulting in
infiltration staining (Fig10).
Transillumination While the opacity of dentin provides
and establishes a baseline for value, the
Transillumination reveals histo-anatomic luminosity is predominately regulated by
relative opacity levels (transparent den- the properties of enamel. The surface
tin vs opaque) and visual quantitative texture influences the primary interac-

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CENTRAL

LATERAL

CANINE

Fig 18 Stone replicas facilitate visual assessment and rumination of the variability between enamel and
dentin surface topography. Mesiobccal (left) and Mesiopalatal (right) oblique views of central incisor,
lateral incisor and canine (top to bottom). Generalized external enamel macromorphological congruency
is seen upon the dentin counterpart, with amplified vertical corrugations, providing added roughness and
waviness that is critical to be emulated during restorative stratification techniques.64

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tion of the incident light. A highly tex- they opt to employ; a trilaminar technique
tured surface renders higher amounts of (enamel/DEC/dentin) for a simplex inter-
diffuse surface scattering, thus elevat- pretation or a pentalaminar technique
ing the perceived value, appearing to (Exo enamel/eso enamel/DEC/exo den-
be more translucent. This is in contrast tin/eso dentin) for a complex one. The
to a smooth surface, which would ex- utilization of this knowledge in the clin-
hibit lesser amounts of diffuse surface ical and technical restorative approach
scattering, thus demoting the perceived is to be described thoroughly in articles
value, and appearing to be more trans- that will be published in future issues of
parent. The relative thickness of enamel this journal.
dictates the proportional amount of dif-
fuse subsurface scattering (quantita-
tive), while the degree and postmatura- Conclusion
tion stage of the HAp crystals affects the
type of photonic interaction (qualitative). This article presented fundamental yet
Transparency, translucency and simplified photonic interactions with re-
opacification are all visual representa- gards to the histoanatomic elements, ren-
tions of the amount of light that is scat- dering the final visual synthesis. It should
tered and subsequently reflected to the be emphasized that a thorough under-
observer by the microstructural features standing of the light propagation within
of a given substrate. Embracing the the coronal structures is a prerequisite
paradigm shift of thinking in terms of dy- in order to elucidate color and shade,
namic light interactivity via the principle however mastery of spatial distribution
of scattering enables the clinician and of the three-dimensional histoanatomic
technician to choose the level of sophis- relationships is paramount in the quest
tication within the stratification protocol for restorative dental emulation (Fig18).

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