Abstracts / 51 (2015) e1e36
cancer. The purpose of this study was to evaluate if tumour budding
can be a prognostic factor for OSCCs.
Methods: Tumour-tissue from 58 patients with primary, small
OSCC, at stages T1/T2, N0, and M0, was collected from the ENT
department, Rikshospitalet, Oslo. After diagnosis, patients were pri-
marily treated by surgery and 62% with postoperative radiotherapy.
Follow-up information was obtained from their medical records.
Budding was detected by immunohistochemistry using
anti-pan-keratin antibodies, and tumour budding was scored in the
most invasive part of the tumour, registrating the eld with most buds
(200 magnication). Findings were correlated with treatment and
follow-up information.
Findings: All the carcinomas stained positively for pan-keratin. The
tumours were split into two groups: low-budding (<5 buds) and
high-budding (P5 buds). The number of buds were correlated with
follow-up status, which displayed a signicantly higher number of
recurrences among patients with ve or more buds (p = 0.043).
Comparing the high-budding tumours in relation to the type of treat-
ment type (had surgery only or postoperative radiation), there was a
trend of fewer relapses within the postoperative-radiation group,
although it was not statistically signicant. When comparing various
clinicopathological variables, tumour budding was the only signicant
prognostic factor in Cox multivariate analysis.
Interpretation: Tumour budding in the tumour front was detected in
most of the OSCCs, and a high number of buds were associated with a
higher frequency of recurrent disease. This indicates a possible clinical
value for use of such scoring.
http://dx.doi.org/10.1016/j.ejca.2015.06.057
P0095
TRENDS IN CURE PROPORTION OF MAJOR CANCER SITES
IN JAPAN BETWEEN 1993 AND 2006 (J-CANSIS STUDY): AN
OBSERVATIONAL STUDY
Y. Ito a,*, I. Miyashiro a, M. Hattori b, Y. Nishino c, A. Ioka a, T.
Nakayama a, B. Rachet d. a Center for Cancer Control and Statistics,
Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka,
Japan, b Department of Cancer Therapy Center, Fukui Prefectural
Hospital, Fukui, Japan, c Division of Epidemiology, Miyagi Cancer
Center, Research Institute, Natori, Japan, d Cancer Research UK
Cancer Survival Group, Department of Non-Communicable Disease
Epidemiology, London, UK
Background: Cure proportion is an important statistic for cancer
patients and clinicians. However, these data were scarce in Japan
and other Asian countries. We aimed to report trends in cure propor-
tion and median survival time (MST) for patients who still have cancer
after treatment (uncured patients) to evaluate the improvement in can-
cer treatment in Japan.
Methods: The J-CANSIS study was a collaborative observational
study of cancer survival in Japan, and used population-based cancer
registry data. We used 791,284 patients data from six prefectural can-
cer registries (Yamagata, Miyagi, Fukui, Niigata, Osaka, and
Nagasaki). We applied exible parametric cure models to estimate cure
proportion and MST for uncured patients with major cancer types (ie,
stomach, colorectal, liver, lung, breast, cervix, and ovary) at diagnosis
(during 199397, 19982001, and 200206), sex, and by age. We
selected an adequate model based on its tting and likelihood-ratio test
for all six cancer sites.
Findings: Cure parameters could not be estimated for uncured
patients with breast cancer and the cure models tted poorly to those
e19
with liver cancer (all age groups) or colorectal cancer (aged 75 years or
older). The cure proportion of patients with stomach cancer was not
substantially improved over the diagnosis periods; MST increased by
a few months in the latest period (200206). From rst to last period
the cure proportion of those with ovarian cancer improved by 5.8
12.9%. Lung cancer showed dierent trends among sexes; the cure pro-
portion improved more for women (9.514.7%) than for men (2.9
6.5%).
Interpretation: Cure proportion for women with ovarian cancer or
lung cancer improved considerably, which was probably due to the
improvement in cancer treatment with time.
http://dx.doi.org/10.1016/j.ejca.2015.06.058
P0098
ASSOCIATION OF SPECIAL DIETARY HABITS WITH
ABERRANT METHYLATION OF P16, E-CADHERIN, AND
HMLH1 GENES IN GASTRIC CARCINOMA IN KASHMIR
VALLEY, INDIA
M.A. Banday a,*, K. Alam a, M.R. Mir b. a Department of Medical
Oncology and General Surgery, SK Institute of Medical Sciences, Soura,
Srinagar, India, b Clinical Biochemistry, SKAUST, Kashmir, India
Background: Kashmir valley (India) has a high incidence of gastric
cancer, suggesting that special dietary habits of the Kashmiri popula-
tion diet consumption (including traditional pickles, sundried food,
and mixed spice cake) could be a predisposing factor for gastric carci-
noma. This study aimed to correlate some special dietary habits of
patients with gastric carcinoma in Kashmir with the hypermethylation
of three important cancer related genes (p16, hMLH1, and E-cadherin).
Methods: DNA was extracted from the surgical samples of 65
patients with gastric carcinoma and hypermethylation. Specic PCR
(MSP-PCR) was used to reveal the hypermethylation of p16,
hMLH1, and E-cadherin genes. The relationship between the
hypermethylation of these genes particularly with dietary habits and
with other patient characteristics, were evaluated.
Findings: Aberrant gene methylation of p16, E-cadherin, and
hMLH1 were 40%, 58%, and 80%, respectively. A signicant
age-related association (older than 50 years) was observed with
hypermethylation of hMLH1 (p = 0.003) and p16 (p = 0.01).
Furthermore, a highly signicant association was noted between dier-
entiated histological type of tumour and hMLH1 gene methylation
(p = 0.0001), whereas methylated E-cadherin displayed a fairly signif-
icant association with dierentiated histological types of tumours
(p = 0.02). A strong association was observed between smokers and
hMLH1 (p = 0.002) and E-cadherin (p = 0.04) gene methylation.
Interestingly, p16 gene methylation showed a highly signicant
association with eating traditional pickle (p = 0.0001) and mixed
spice cake (p = 0.004), which was the same for E-cadherin gene
methylation (p = 0.01). Sundried vegetable consumption was also
found to be strongly associated with hMLH1 gene methylation
(p = 0.0001).
Interpretation: The special dietary habits of the Kashmiri popula-
tion probably plays an important part in induction of methylation of
genes p16, hMLH1, and E-cadherin, and thus to the development of
gastric carcinoma.
http://dx.doi.org/10.1016/j.ejca.2015.06.059