Lahore University of Management Sciences

lBIO 212 Biochemistry

Spring 2016
Instructor Dr Syed Shahzad Ul Hussan, Dr Ahmed Jawaad Afzal
Room No. 9-4178, 9-316
Office Hours
Email Shahzad.hussan@lums.edu.pk; ahmed.afzal@lums.edu.pk
Telephone
Secretary/TA
TA Office
Hours
Course URL (if
any)

Course Basics
Credit Hours 3
Lecture(s) Nbr of Lec(s) 2 Duration 75 minutes each
Per Week
Recitation (per week) Nbr of Rec (s) 1 Duration
Per Week
Lab (if any ) per Nbr of Duration
week Session(s) Per
Week
Tutorial (per week) Nbr of Tut(s) Per Duration
Week

Course Distribution
Core Core
Elective
Open for Student Open but capped at (maximum # of students you can have in class)
Category
Closed for Student Non-Biology/Chemistry majors
Category

COURSE DESCRIPTION

The Biochemistry Sophomore course is designed to provide undergraduate students an understanding of

fundamental concepts in Biochemistry. Students will learn molecular mechanisms underlying biological
processes, fundamental principles that drive biochemical reactions, molecular organization starting with the
monomeric subunits, structures and how structure relates to function, different techniques in studying
structural properties and finally the metabolic pathways of biological system.

COURSE PREREQUISITE(S)

Introductory Biology

COURSE OBJECTIVES
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To understand fundamental biochemical concept, processes, and structure-function relationship
To have basic idea of various methods and techniques involved in genomics, proteomics and
bioinformatics.

Learning Outcomes
At the end of the course, students should be able to comprehend the basic biochemical processes and
concepts involved in various biological systems.
Students will understand the interaction of macromolecules involved in biological processes, chemical
bonding, enzyme kinetics, structure, folding and function.
Additionally, students will demonstrate their knowledge of basic proteomics and bioinformatics and
various techniques associated with protein purification and structural analysis.
Grading Breakup and Policy

Assignment(s): 2 (5% each)

Quiz(s): 20% (N-2 policy)
Attendance: 10% (disruptive behavior will be penalized)
Midterm Examination: 30%
Final Examination: 30 %

Examination Detail

Yes/No: yes
Midterm Combine Separate: separate (not cumulative)
Exam Duration: I hr 10 min
Preferred Date:
Exam Specifications: multiple choice questions, true and false and descriptive
Yes/No:
Final Combine Separate: separate
Exam Duration: 1 hr 10 min
Exam Specifications: multiple choice questions and true and false and descriptive

Recommended Reading:
Topics Instructor LehningerL or Voet and VoetV
Chapter; Page No
Foundation of Biochemistry:
Lecture 1 cellular, chemical, physical
AJA Ch.1; Pg. 1-30L
24-01-2017 and genetic foundation

Lecture 2 Proteomics: Protein

AJA
26-01-2017 detection and Interaction Ch.9; Pg. 333-339L

Lecture 3 Water, acids and bases

31-01-2017 SHZ Ch. 2; Pg. 43-70

Lecture 4 Amino acids, peptides and

SHZ CH. 3; Pg. 71-112
2-02-2017
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proteins; and Primary
structure of proteins

Lecture 5 Three dimensional structure

07-02-2017 of proteins SHZ Ch. 4; pg. 113-140

Lecture 6 X-ray and NMR structures

09-02-2017 SHZ Additional reading

Bioinformatics: In silico
Lecture 7 methods for
14-02-2017 Ch. 4; Pg. 115-141L + Additional
Structure prediction AJA
reading
Alignment algorithms

Methods in protein
quantification
Lecture 8
Gel electrophoresis (native, Ch. 3; Pg. 90-96L,
16-02-2017 AJA
Ch.6; Pg. 147-156V
SDS and 2D gel
+ Additional reading
electrophoresis, DR PAGE,
Western Blots), ITRAQ

Post-translational
Lecture 9
modifications: Glycosylation AJA Ch. 10; Pg. 357-368L
21-02-2017
(Akhlaq) Ch. 12; Pg. 386-393V

Lecture 10 Enzyme catalysis AJA Ch.6; Pg. 192-213L

23-02-2017 Ch.15; Pg. 506-517V

Lecture 11 SHZ
Ch. 6; Pg. 183-203
28-02-2017 Enzyme kinetics

Lecture 12
SHZ CH. 6; Pg. 204-227 + additional
02-03-2017
Enzyme inhibition reading

Lecture 13
07-03-2017 SHZ
Ch.7; Pg. 235-251
Carbohydrates

Lecture 14 Glycobiology, glycoproteins

SHZ
09-03-2017 and glycolipids Ch. 7; Pg. 252-263
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10-03-2015 Midterm Exam

Lecture 15 Protein Phosphorylation and
14-03-2017 signal transduction AJA Ch. 12; Pg. 433- 472L
Ch. 19; Pg. 671- 699V

Lecture 16 Protein Mis-folding and Prion

AJA Ch. 4; Pg. 143-151L
16-03-2017 diseases

Lecture 17 Prions (2)

AJA
28-03-2017 Ch. 9; Pg. 306-316V

Lecture 18 Bioenergetics and

AJA
30-03-2017 biochemical reaction types Ch. 13; Pg. 505-535L

Lecture 19 Carbohydrate biosynthesis in

AJA Ch. 20; Pg. 799-810L
04-04-2017 plants (Photosynthesis) Ch. 24; Pg. 901-938V
Lecture 20 Glycolysis SHZ
Ch. 14; Pg. 551-568
06-04-2017
Lecture 21 Metabolic regulation SHZ
Ch. 16; Pg. 615-629
11-04-2017
Lecture 22 Fatty acid catabolism
SHZ
13-04-2017 Ch. 14; Pg. 5528-547

Lecture 23 Amino acid oxidation

SHZ
18-04-2017 Ch. 18; Pg. 673-701

Lecture 24 Oxidative phosphorylation

SHZ
20-04-2017 and photophosphorylation Ch. 19; Pg. 707-760

Gluconeogenesis and
Lecture 25
pentose phosphate SHZ
25-04-2017 Ch. 15; Pg. 570-610
pathways

Lecture 26 Citric acid cycle

SHZ
27-04-2017 Ch. 17; Pg. 647-667

Lecture 27 Lipid biosynthesis

SHZ
02-05-2017 Ch. 21; Pg. 805-840

Lecture 28 Hormonal regulation in

SHZ
04-05-2017 mammalian metabolism Ch. 23; 901-945

Final Exam
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Recommended reading: Principles of Biochemistry, 6th edition, by Lehninger and

Biochemistry, 4th edition by Voet and Voet