Академический Документы
Профессиональный Документы
Культура Документы
10 mg drug was dissolved in pH 7.4 Phosphate Buffer, to prepared 100 ug/ml solution
Step 3: After taking absorbance, to plot graph in Excel, Click Insert Chart Scatter
Scatter
Chart
Select Graph
Absorbance of Prepared
Concentrations (10 g/ml)
Generated Graph
Title
Axis Title
Generate Equation
Y = mx + c
Where, Y = Absorbance,
m= Slope or gradient,
x = Concentration,
c = Y intercept,
Write click
Add Trendline
computer screen
computer screen
0 0 0 0 0 0 0 0 0 0
IMPORTANT NOTE:
1. In every calculation in Excel sheet, Always starting the calculation with = this sine
(equal to sine), if youre not used this sine calculation was not done.
2. After complete all calculation to press ENTER button to give your reading.