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Pharmaceutical Analysis:
Series of tests to determine identity, purity, stability, toxicity, physical and chemical properties, and interactions, along with
the structure elucidation of drugs and related substances.
C. Gasometric Analysis:
P: Calculating amount of gases present in the sample.
D: CO2 and NO2 gases
D: Determination of density, specific gravity, viscosity or conductivity of any drug or related product.
A. Potentiometry:
P: The principle involved in the potentiometry is when the pair of electrodes is placed in the sample solution it shows
the potential difference by the addition of the titrant or by the change in the concentration of the ions.
D: % of acetyl salicylic acid in aspirin tablets, determination of the carbonate.
B. Polarography/Voltammetry:
P: The main principle in the polarography is the reduction process taking place at the electrode. This method has
limited sensitivity. The reduction at the electrode increases the voltage applied between the polarisable and non-
polarisable electrodes and the current is recorded that is, the metallic ions are reduced at the surface of the
electrode.
D: Vitamin C, Local anaesthetics
C. Absorption Techniques/Opticometric Methods:
a. UV-Visible Spectrophotometry:
P: measurement of interaction of electromagnetic radiation with the quantised matter at specific energy levels.
D: Diazepam, Acetazolamide, Paracetamol, Riboflavin
b. Mass Spectrophotometry:
P: Mass spectrometry (MS) is an analytical technique that ionizes chemical species and sorts the ions based on
their mass-to-charge ratio. In simpler terms, a mass spectrum measures the masses within a sample.
D: Propafenone, Amiodarone
c. IR Spectrophotometry:
P: The absorption is mainly based on the vibrations of the chemical substances after absorption of the infrared
radiation. The spectrum bands present in the IR spectrum represent the functional groups and bonds present in
the chemical substance. Hence, the IR spectrum is commonly known as the finger print of the chemical
substance.
D: identify functional groups of drugs, kinetics studies.
d. NMR spectroscopy:
P: measurement and interpretation of the radiofrequency which induces the transitions in the nuclei by
absorbing the radiofrequency waves and the spectra are known as the nuclear magnetic resonance spectra.
D: non-destructive analysis of aminoacids, proteins, RNA and DNA, number of carbon atoms present in the
sample.
e. Atomic Absorption spectroscopy:
P: The atoms present in the ground state absorb the radiation produced by the flame and excited to the excited
state and which is measured by the AAS spectrometer. Atomic absorption measures the amount of light at the
wavelength which is absorbed by the atoms. Atoms present in the sample are directly proportional to the
absorption intensity.
D: Arsenic, Trace elements in substances.
D. Separation Techniues:
a. Chromatography:
P: Separation of constituents of a sample due to difference in polarity or affinity.
D: Sensitive separation through high efficiency techniques.
b. Solvent Extraction:
P: Separation of components of a sample based on its miscibility
D: Separation of small batches of sample