Mung Bean Experimental Design

Project Purpose: To involve students in a real world scientific investigation in which they design a controlled
experiment in which only one variable is tested.

Problem: What household ingredient, when added to water, will cause mung beans to germinate and grow.

Group Members
Tatum Okamoto is responsible for Control Group 1

Nina Delos Santos is responsible for Control Group 2

Lele Meeken is responsible for Experimental Group 1

Billy Joe is responsible for Experimental Group 2

**Joella Mae Corpuz - Group Manager**

Top 3 Choices! Look over the research each group member compiled. Complete the table below on your TOP 3 choices.
Household ingredient #1 Sugar Household ingredient #2 Cinnamon Household ingredient #3 Coffee

What did you learn about this ingredient? What did you learn about this ingredient? What did you learn about this ingredient?

I learned that sugar comes in many forms -
sucrose, fructose, glucose- and and is a
necessity in both plant and animal lives.
Sugars are produced from plants after
photosynthesis and is used to generate
energy; this energy takes form as four plant
enzymes being Proteases, Amylases,
Lipases, and Cellulases. Focusing, on the
I learned that cinnamon can help grow
sprouts rapidly and can be a defense against
bacteria and disease
Coffee has a caffeine content ranging from
1.0% - 2.5% based on its weight. There are
also low concentration levels of theophylline,
theobromine, paraxanthine, libertine, and
methyllibertine (other alkaloids). Proteins
account for 8% - 12% of the bean. Amino
acids, carbohydrates, 11.7 - 14 g/100 g of
lipids, and antioxidants are also present in
enzyme Amylases which reduces
polysaccharides into disaccharides- lactose,
maltose, and sucrose. With that said Sugar is
a common carbohydrate found in fruits and
vegetables.
Why would this ingredient cause mung beans
to germinate and grow?
Sucrose (glucose and fructose) is a major
product of photosynthesis, a process that
allows plants to turn sunlight, carbon dioxide,
and water into energy, that is used to grow
and germinate plants. With that said as the
seed soaks up water, its food stored inside
begins to be converted into energy in the
form of enzymes. One of these enzymes is
Amylases which reduces polysaccharides (a
carbohydrate that consists of a number of
sugar molecules) into lactose, maltose, and
sucrose. So with Sucrose already present the
process of germination will occur in the
mung bean.
Additional information learned from
research
Sugar is used to create energy in all living
things. Both in plants through photosynthesis,
and in animals that need to find food and as
Why would this ingredient cause mung beans
to germinate and grow?
The cinnamon will encourage the bean to
produce more stems, while helping to
prevent the fungus that causes damping-off
disease, It will make the mung bean safe and
grow at a fast pace Perfect! :) it can be used
as an air freshener and an ant and moth
repellant.it might reduce inflammation or
have antioxidant effects. It's all natural
Straight from trees
Additional information learned from
research
Cinnamon can help fight bacteria in foods
and prevent spoilage. It can help prevent
fungus that causes damping-off diseases.
coffee.
Why would this ingredient cause mung beans
to germinate and grow?
Caffeine is an alkaloid. Alkaloids are natural
occurring chemical compounds and are
typically produced by bacteria, fungi, plants,
and animals. All these are components that
help nurture growth of plants and give it the
nutrients it needs to grow. The coffee will act
as a fertilizer and create an environment
where the mung bean can sprout.
Additional information learned from
research
Coffee grounds can be used to fertilize plants
and keep away small creatures that can
potentially feed on them. Using used (then
rinsed) coffee grounds can help with the pH
 the food is digested it is broken down into levels of soil and the plant. Its also organic
sugars used for energy. which is great because theres no harsh
chemicals added!

Team hypothesis (If and then statement
please): If, we add sugar to water, then the
mung beans will germinate and grow by
providing pre-ready sucrose to the forming
energy enzymes then, the mung beans will
produce the energy it needs for cellular
growth.
Team hypothesis (If and then statement Team hypothesis (If and then statement
please): lf cinnamon can have an effect on please): If we add coffee to water and mung
producing stems more rapidly also a defense beans, then the mung beans will germinate
against disease then the mung bean can faster with the coffee and water mixture
grow faster and healthier. :D than the water itself, due to exposure to
additional content. :3

Title: Using Coffee Grounds to Grow Mung Beans

I. Problem:

What household ingredient when added to water will cause mung beans to germinate and grow?

II. Team Hypothesis:

If we add coffee to water, it will cause the mung beans to germinate faster and grow quicker than just water itself.

III. Experiment
A. Materials
Water 40 Mung beans Coffee grounds
Petri-dishes (4) Paper towel Post-it notes
Tweezers Beakers Graduated Cylinder
 Glass stirring rod Ruler Camera

B. Procedure
Step 1: Label petri dishes with group names (2 control group, 2 experimental group) with post-it notes.
Step 2: Place 10 Mung beans onto 2 separate Petri-dishes (10 in each group, 10 for control group, and
10 for experimental group)
Step 3: Pour 25 mL of plain water into the 4 petri dishes labeled control group and experimental
group
Step 4: Measure 1.0 g of coffee grounds and pour into the 2 Petri-dishes labeled Experimental Group
Step 5: Transfer Petri-dishes by the window to give them sunlight
Step 6: Wait for the next day
Step 7: Take picture of mung bean growth results from the day
Step 8: Rinse the Mung beans gently under stream of clean water.
Step 9: Record any growth changes of the mung beans.
Step 10: Calculate the average growth of the mung beans for the day.
Step 11: Repeat steps 5-11 the next day.

C. The Controlled Experiment

1. Describe the control group
The control group will consist of the mung beans that will grow in plain water.

2. Describe the experimental group

The experimental group will be the mung beans in the petri-dish that will grow in the coffee
 grounds & water mixture.

3. Identify the controlled variables of the experiment.

Amount of water, amount of mung bean seeds, time picture should be taken, amount of sunlight,
placed next to a window, same room temperature

4. Describe the independent variable

The independent variable is the coffee grounds & water mixture.

5. Describe the dependent variable

The dependent variable will be the amount of the mung beans that germinated/mung bean
growth in the course of 1 week.

IV. Organizing the Data -

A. Recorded Data -
Growth of Beans of Control Group 1
Growth of Beans (mm) Averag-
e
Growth
of Bean
Day 1 0 0 0 0 0 0 0 0 0 0 0
Day 2 0 11 9 8 10 10 12 8 8 6 8.2
Day 3 10 10 15 12 15 24 45 28 38 40 21.6
Day 4 30 64 45 40 68 40 15 10 25 10 34.7
Day 5 80 55 110 57 64 15 18 33 53 5 49
 Growth of Beans of Control Group 2
Growth of Beans (mm) Averag-
e
Growth
of Bean
Day 1 0 0 0 0 0 0 0 0 0 0 0
Day 2 0 10 10 11 12 14 7 9 15 10 9.8
Day 3 25 29 42 36 15 32 10 2 38 28 25.7
Day 4 53 56 51 33 66 72 4 28 26 46 43.5
Day 5 102 81 61 80 26 30 61 89 31 - 62.3

Growth of Beans of Experimental Group 1

Growth of Beans (mm) Averag-
e
Growth
of Bean
Day 1 0 0 0 0 0 0 0 0 0 0 0
Day 2 1 1 2 2 4 0 0 0 0 0 1
Day 3 4 2 4 2 1 1 0 0 0 0 1
Day 4 4 2 - - - - - - - - 1
Day 5 4 2 - - - - - - - - 1
 Growth of Beans of Experimental Group 2
Growth of Beans (mm) Averag-
e
Growth
of Bean
Day 1 0 0 0 0 0 0 0 0 0 0 0
Day 2 1 3 3 5 0 0 0 0 0 0 1.2
Day 3 4 2 1 1 1 0 0 0 0 0 1
Day 4 4 2 4 - - - - - - - 1
Day 5 4 2 4 - - - - - - - 1
B. Processed Data -

Average Growth of Mung Beans

Control Group 1 Control Group 2 Experimental Experimental Total Day
Group 1 Group 2 Average
Day 1 0 0 0 0 0
Day 2 8.2 9.8 1 1.2 5.05
Day 3 21.6 25.7 1 1 12.32
Day 4 34.7 43.5 1 1 20.05
Day 5 49 62.3 1 1 28.32

Total Group 22.7 28.26 0.8 0.84 13.15

Average
C. Graphs -