You are on page 1of 316

Product Catalogue & Laboratory Guide

Contact Information Ordering by Phone, Fax or E-mail


New and returning customers can place orders by
Contact Us By Mail phone, fax, or by e-mailing sales@phytotechlab.com.
PhytoTechnology Laboratories
PO Box 12205 When submitting an order, please make sure to
Shawnee Mission, KS provide the following information:
66282-2205 Shipping and Billing Information
United States of America Shipping Address with Country
Billing Address with Country
Company Name (if applicable)
Contact Us by Phone or Fax Billing Contact Name
Phone: 1.913.341.5343 Phone Number
1.888.749.8682* E-mail Address
Fax: 1.913.341.5442 Customer VAT Number (EU Only)
1.888.449.8682* Product Information
Product Catalogue (ID) Number
Product Description
*Toll free numbers are applicable for the United Package Size
States, Canada, and some parts of the Caribbean.
Contact Information

Quantity to be ordered
Credit Card Information
Shipping Address Name on Credit Card
PhytoTechnology Laboratories Billing Address with Country
9245 Flint Street Credit Card Number
Overland Park, KS Expiration Date
Three or Four Digit Security Number (SSV)
66214-1739 Miscellaneous Information
United States of America PO Number (if applicable)
Special Shipping Instructions (if applicable)
Sales Information
Questions regarding pricing, product availability, Hours of Operation
or shipping and returns policies can be answered by Our hours of operation are 8:00 am through 5:30 pm,
contacting: sales@phytotechlab.com. Monday through Thursday and 8:00 am and through
1:00 pm on Friday. Our offices are closed on Saturday
and Sunday. PhytoTechnology Laboratories operates
Technical Inquiries in the Central Time Zone (UTC -0600).
Technical inquiries regarding our products (e.g.,
questions about product usage, custom media PhytoTechnology Laboratories is closed on the
quotations, bulk pricing, or recommended following holidays:
concentrations) or requests for any technical Memorial Day (Last Monday in May)
documentation (e.g., MSDSs or Certificates of Independence Day (4 July)
Analysis) can be directed to: tech@phytotechlab.com. Labor Day (First Monday in September)
Thanksgiving (4th Thursday & Friday in
November )
For Questions about Orders Winter Holidays (23 December to 02 January)
For any questions about an order that has already been
placed, please contact: cs@phytotechlab.com. Follow Us On:
Placing an Order
Ordering Online
For your convenience PhytoTechnology Laboratories For more information about PhytoTechnology
accepts orders online through www.phytotechlab.com.
Laboratories products and services, for expert
Rush orders (i.e., requiring same day shipping) should
be placed by phone to ensure availability.
technical information, or to place an order, be sure
to visit www.phytotechlab.com.

2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
General Information: 2-14
Ordering and general company & catalogue information.

Biochemicals: 15-56
General use biochemicals for use in the plant sciences.

Plant Tissue Culture Media: 57-130


Growth media fir plant tissue culture and micropropagation.

Molecular Biology: 131-150


Buffers, Reagents, and Antibiotics for use in plant molecular biology.

Microbiology: 151-159

Table of Contents
Bacterial and fungal growth media for microbiology research.

Phytopathology: 161-164
Growth media for phytopathology research.

Seed Testing: 165-172


Media, antibiotics, stains and dyes for use in seed testing.

Phycology: 173-178
Media and biochemicals for use in algal biomass production and research.

Kits: 179-185
Educational classroom kits for demonstrating plant tissue culture.

Equipment: 187-218
Laboratory equipment for all fields of plant science and general use.

Technical Information: 219-293


Basic technical information on laboratory set up, tissue culture
techniques, product applications, and more. Media formulation
tables on pages 270 to 293.

Indices: 294-311
Technical Figures and Tables Index.
Alphabetical Index of all PhytoTechnology Laboratories Products.

Table of Contents Page 1


Product Catalogue & Laboratory Guide
About PhytoTechnology Sterile Liquids
PhytoTechnology Laboratories offers a range of sterile
Laboratories liquids, including: antibiotic solutions, plant growth
PhytoTechnology Laboratories was established in 1998, regulator stock solutions, vitamin solutions, liquid
by Dr. Kenneth C. Torres, to provide a new source for media and more. Each solution is manufactured in-
pre-manufactured plant tissue culture media. In house and then filtered through a 0.2 m filter. All
the years since, PhytoTechnology Laboratories has sterile filtered liquids are confirmed sterile according
grown to provide a wide range of products for the to USP<71> protocols.
plant sciences, including: plant tissue culture, plant
molecular biology, phytopathology, seed testing,as well Physio-Chemical Testing
as microbiology, and phycology. Prior to packaging, or use in manufacturing, all
biochemicals must pass a series of physio-chemical
For over a decade, one thing has set PhytoTechnology testing. These tests are designed to ensure the product
Laboratories apart from its competitors: an unwavering adheres to set specifications for: solubility, appearance,
dedication to quality. By providing quality products of pH, and FTIR or HPLC identity confirmation.
consistent excellence, PhytoTechnology Laboratories
enables their customers to produce controlled and Biological Testing
General Information

repeatable results in experimental research and helps The vast majority of our biochemicals and media are
mass micropropagationists produce larger yields of then tested on commercially significant and product
plants through plant tissue culture. appropriate plant cell lines, to ensure conformity to
expected standards and promote plant growth.
PhytoTechnology Laboratories offers the world's
largest selection of media for plant tissue culture, and
ISO Certification
with the other product lines offered, carries over 900+
products for plant sciences applications. They also
offer a range of proprietary products including unique
equipment like our ergonomic Glass Bead Sterilizers
(product numbers S7510/S7520) and single-use
disposable culture vessels (product numbers C1958/
C2118/C1932).

PhytoTech Standards of Quality PhytoTechnology Laboratories was one of the first


small business suppliers for the plant sciences to
Biochemicals attain and maintain its ISO Certification. As an
PhytoTechnology Laboratories takes extra steps to ISO 9001:2008 registered firm, PhytoTechnology
source biochemicals of the highest quality for sale and Laboratories must pass an annual outside audit of
manufacture. Biochemicals meet either United States our manufacturing and quality control processes
Pharmacopeia (USP), European Pharmacopeia (EP), to maintain this rating. An ISO certification is an
or American Chemical Society (ACS) specifications, independent and outside confirmation that you will
as applicable to the product. be receiving products of superior quality when you
purchase from PhytoTechnology Laboratories.
Media
PhytoTechnology Laboratories media is manufactured
in-house at our main campus in Overland Park,
KS. All media is manufactured in accordance with
current Good Manufacturing Processes (cGMP)
guidelines with a proprietary inventory tracking and
management system that ensures minimal lot-to-lot
variation in media production.

Page 2 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
Understanding Catalogue Listings
Generally speaking, this catalogue uses two types of
catalogue listings for chemicals. The first listing is for
products that consist of one chemical, or chemical solution.
Denotes new product since last
New Product Product Number & Name
catalogue. For your convenience, the product
G3350 - Gentamicin Solution (50 mg/mL) number and name link to the product
Gentamycin Sulfate web page
Product notes, full chemical
Aqueous Solution
name, and common aliases.
Sterile Filtered
Liquid Dry Ice, Chemical Hazards and
Form, CAS Number, Formula,
Molecular Weight, Solubility, & Storage CAS Number: 1405-41-0 Hazardous Shipping Symbols.
Temperature. Formula: C21H43N5O7 H2SO4 See "Catalogue Symbols" on page 11
Molecular Weight: 575.67 for more information.
Available Package Sizes Miscible with Water
Listed from smallest to largest.
10LFB Indicates a single-use foil bag
Store at 2 to 6 C
instead of a plastic bottle.
10x1mL A package of ten bottles in
Available Package Sizes

General Information
1mL aliquots. 10mL 25mL 100mL
Unless a "liquid" symbol is listed,
1L indicates the amount of powder
needed for a 1L solutions.
Package sizes link to the product web
page.
The second type of listing is for products that are
manufactured by PhytoTechnology Laboratories from
multiple chemicals, specifically media. This listing
provides the formulation of the product. Product Number & Name
For your convenience, the product
L5138 - LB Broth, Lennox L number and name link to the product
Contains the nutrients as described by Bertani (1951), and web page
Formulation reference, product Luria and Burrows (1955). Formulation modified by Lennox L
notes and aliases. (1955).
Also known as Lysogeny Broth or Luria-Bertani Broth
Store from 2 to 6 C
Soluble in Water Storage Temperature, Solubility
Use at 20.00 grams per liter and usage in grams per liter.
Formulation Legend Components (mg/L)
Most listings are mg/L
Sodium Chloride 5000
Tryptone 10,000
Available Package Sizes Yeast Extract 5000 Formulation., component name
Listed from smallest to largest. and amount in mg per liter.
10LFB Indicates a single-use foil bag Approximate pH at Room Temperature 6.0 - 7.5
instead of a plastic bottle.
10x1mL A package of ten bottles in
Available Package Sizes
1mL aliquots. 1L 500g 5Kg
Unless a "liquid" symbol is listed,

Catalogue Listings Glossary


1L indicates the amount of powder
needed for a 1L solutions.
Package sizes link to the product web
page. Sterile Filtered Solution has been filtered through a 0.2 m filter. Product is
confirmed sterile according to USP<71> protocols.
Use at xx.x grams per liter amount in grams of the product required to make
a 1x solution in 1 liter of water.
Solutions Unless otherwise indicated, all solutions are prepared in DI Water.
Products are listed alphabetically in the catalogue. Indexes by CAS Number
and by Product Number can be found at the back of the catalogue.

General Information Page 3


Product Catalogue & Laboratory Guide
Understanding our Labels
All products sold by PhytoTechnology Laboratories are labeled in a consistent fashion, which is designed to
provide any and all pertinent information about the product. PhytoTechnology Laboratories makes every
attempt to provide the most accurate product information possible, over time the specifics of our labels may
change to keep in adherence with current government and international regulations. This section is intended to
be a general guideline to our labeling practices.

A D

B
C

E
General Information

F H

A. Product Number

B. Package SizeThis will indicate the minimum quantity of product in the package. For pre-weighed media, this will be the amount of powder
required to prepare the media in the volume listed. The user should always measure the amount needed from the container.

C. Lot Number

D. Product NameThis will indicate the products name as well as specific descriptions or synonyms.

E. Storage Temperature, Usage, Chemical Formula, Formula (Molecular) Weight, CAS Number, UN Number, and Harmonized
Commodities CodeStorage temperatures indicated are for long-term storage of products (Products may be shipped under different
conditions to reduce shipping cost, while still ensuring product quality).

Usage is used for products that have a specific usage requirements. Most commonly this appears on labels for plant tissue culture or
microbiology media and the usage indicates the prescribed grams per liter usage to prepare one liter of media.

The Chemical Abstract Service (CAS) number is shown whenever available. CAS numbers vary in how specifically they describe a product,
therefore, more than one CAS number may have been assigned to a product. Some chemicals have a UN Number which was assigned
them by the United Nations. It relates to how hazardous a material may be, and can differ between liquids and solids of the same chemical.
Harmonized Commodities Codes are schedule B export codes that can be specific to a chemical or a family of chemicals.

F. QR Code: Depending on the product, the QR code can take you to either the product's Certificate of Analysis for the specific lot, the
Material Safety Data Sheet for the product, or to the Product Information Sheet.

G. Hazard InformationThis provides a more complete description of the hazardous nature (if any), handling precautions, and emergency
procedures that need to be followed when handling this product. Risk and Safety values are listed below this information. The user should
always consult the Material Safety Data Sheet (MSDS) prior to the use of any product. See "Catalogue Symbols" on page 11 for a list of
hazardous pictograms used in this catalogue and on our labels.

H. Hazard PictogramThis lets the user know at a glance what safety hazards exist with this product. If no symbol is required, this diamond
will indicate so by "No GHS Symbol."

Page 4 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
PhytoTechnology Laboratories
PhytoDirect EU Distribution Programme

Door to Door distribution of products; most delivered in 3-4 Business Days;


Shipping includes clearing all products through customs and paying all duties and
VAT (when provided with customer VAT);
Flat rate shipping of $25.00 USD for all orders of $250.00 - 1000.00 USD of select
products and package sizes;

General Information
Free shipping on orders greater than $1000.00 USD of selected products and
package sizes;
Orders may be placed online at www.phytotechlab.eu.com or by e-mail or fax.
E-mail confirmation and tracking number provided with each order.
Programme does not apply to package sizes larger than 5 Kilograms.
Programme is subject to change without notification.

Day One: Day Two: Days Three - Four:


Product Leaves Product arrives in Product Leaves
PhytoTech Labs France and clears distribution facility
in Overland Park, EU customs. Duties in France and is
KS to distribution and VAT are paid. delivered to any EU
facility in France. Member State.

Countries Included in the PhytoDirect EU Distribution Programme


Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Great Britain, Greece, Hungary, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Poland,
Portugal, Romania, Slovak Republic, Slovenia, Spain and Sweden

PhytoTechnology Laboratories provides research quality products for plant tissue culture,
plant molecular biology, and microbiology applications at competitive prices worldwide.
Quality, Innovation, and Service are the backbone of the PhytoTech Philosophy which
has allowed PhytoTechnology Laboratories to be a leader in the plant science market for
over fifteen years. Discover the difference PhytoTech Quality & Service can make!

www.phytotechlab.eu.com
General Information Page 5
Product Catalogue & Laboratory Guide

Organic Media for Potatoes


At the 2013 Society for In Vitro biology meeting, we presented a poster paper (Development of Organic-Based
Micropropagation Media) that outlined the development and testing of our organic potato media. This poster is
available at http://www.phytotechlab.com/pdf/SIVB2013OrganicMedia.pdf. In continuation to that previous study
a comparison between different sources of vitamins has been examined. In this study, Organic Coconut Powder
(Product No. C1780), a natural and organic source of vitamins, was tested against Gamborg Vitamin Solution
1000X (Product No. G219). Additionally, this study evaluates the overall performance of Phyto-Organic Potato
Media (Product No. P6700 and P7000) with Murashige & Skoog (MS) Basal Salts when supplemented with organic
PGRs and non-organic PGRs, respectively.

Figure 1: Results of fresh weights


General Information

after 30 and 60 day periods of potato


tissues on Phyto-Organic Potato Me-
dia 1 and 2. Control (MS) w/G219
was supplemented with 4.4 M BA
and 0.14 M NAA. All organic-
based media were supplemented
with 2.5 mL/L Org. PGR A and 1
m/L Org. PGR B.

Potato Medium 2 w/C1780 @ 15 g/L yielded an increase in fresh weight of 7346% while the MS control had an
increase of 3566%.
Although data collected were the results from one potato cultivar, both Potato Media supplemented with either
G219 or C1780 showed similar or greater increase in fresh weight than those grown on MS w/G219.

Typical concentrations used to obtain the above results are listed in the following table:
Recommended Concentrations Per Liter
Product Description Product Number Amount to use per liter
Phyto-Organic Potato Medium 1 P6700 8.71 g/L
OR
Phyto-Organic Potato Medium 2 P7000 8.88 g/L
Supplemented with components listed below
Organic PGR A (provided with media) 2.5 mL/L
Organic PGR B (provided with media) 1 mL/L
Phyto-Organic Sucrose S7761 30 g/L
Phyto-Organic Coconut Powder C1780 15 g/L
Phyto-Organic Agar A1000 6 g/L
Adjust Final pH to 5.6 5.8

Page 6 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
New from PhytoTechnology Laboratories
Products for Plant Defense Research
Stress resistance, both abiotic and biotic, is a widely researched topic
in plant science. We are introducing a new product line to aid re-
searchers in deciphering Plant Defense and Immunity. This product
line covers mostly biotic stress resistance and the pathogen-triggered
innate arm of plants immune system. In pathogen-triggered immu-
nity there are two-main pathways: Biotrophic (e.g., micro-organism
induced infection) and Necrotrophic (herbivore/wound based infec-
tion), and there is cross-talk between key signals in each pathway.
The most well-characterized biotrophic infection comes from the
N-terminus of the flagellin of Pseudomonas aeruginosa, a small con-
served 22 amino-acid sequence (P6622) that interacts with PAMP
(pathogen-associated molecular patterns) receptors and activates the
MAPK (mitogen-activated protein kinase) pathway to ultimately
induce SAR (systemic acquired resistance). Other biotrophic infec-
tions can utilize the shikimate pathway for accumulation of salicylic

General Information
acid (S7530, S7580) which upregulates PR (pathogenesis-related
proteins) and also leads to SAR.
In necrotrophic infections, the most well characterized pathway re-
sulting from wounding comes from a peptide systemin which exists
in the Solanceae family of plants. This path leads to accumulation of
jasmonic acid (J379) and methyl jasmonate (J389) to warn nearby
plants and express endogenous defensin peptides that often have
anti-microbial and antifungal properties.
Product Package
Number Product Description Size
FLG22 PEPTIDE
Peptide with its sequence derived from the flagellin N-terminus of P. aeruginosa and is known to elicit specific innate 1 mg
P6622 5 mg
immune response in plants as well as animals.

ACETYLSALICYLIC ACID
Hydrolyzes to form salicylic acid (S7530). One of the first exogenously applied chemicals to induce resistance to
A118 100 g
Tobacco Mosaic Virus.

1-AMINOCYCLOPROPANECARBOXYLIC ACID (ACC) 100 mg


ACC is a cyclic -amino acid that is the immediate precursor of the plant hormone ethylene.
A1180 500 mg
1g

2,6-DICHLOROISONICOTINIC ACID (INA)


First synthetic chemical to induce SAR. Like salicylic acid it inhibits catalase to start the cascade of upregulating 1g
D2181 5g
pathogenesis-related genes.

METHYL JASMONATE
Methyl ester of jasmonic acid (J379) which has significant vapor pressure so it can signal induction of defense responses 5g
J389 25 g
to nearby plants.

METHYL SALICYLATE
Involved in plant defense reactions, present in plant tissues as well as being an airborne defense signal. 500 mL
S7785 1L

SALICYLIC ACID
An endogenous signal mediating local and systemic plant defense responses against pathogens. 500 g
S7530 1 Kg

SALICYLIC ACID, SODIUM SALT


An endogenous signal mediating local and systemic plant defense responses against pathogens. A more water soluble 500 g
S7580 1 Kg
form of S7530.

For more information call us at 913.341.5343 or visit www.phytotechlab.com


General Information Page 7
Product Catalogue & Laboratory Guide
Accepted Forms of Payment collection unless prior payment arrangements have
been made and all future orders must be paid in
Credit Cards advance.
PhytoTechnology Laboratories accepts American
Express, Discover, MasterCard and Visa. PhytoTechnology Laboratories is pleased to provide
high-quality products and efficient service at
competitive prices. However, in order to keep prices
competitive, it is necessary for us to be paid in a
timely manner. Thank you in advance for adherence
Other Accepted Forms of Payment to our credit policy.
For your convenience PhytoTechnology Laboratories
also will accept payment by international bank wires,
Electronic Funds Transfer (EFT), and pre-payment by Placing International Orders
business checking. Please contact In addition to ordering through our distributors
sales@phytotechlab.com for more information about PhytoTechnology Laboratories accepts and ships
paying by EFT or international wire. international orders directly from our main campus in
the United States. International orders are shipped via
Orders Requiring Pre-Payment Federal Express shipping, and depending on regional
General Information

First Time Customers: Orders placed by any first- conditions, is either delivered by Federal Express or
time customer require pre-payment. through one of Federal Express's shipping partners
in your home country. International shipping usually
Hobbyists and Personal Orders: Orders by takes somewhere between 3 to 5 days, but individual
hobbyists, individuals, and orders being shipped to shipping times may vary based on regional conditions.
residential addresses require pre-payment.
The customer is responsible for all duties, taxes, fees,
International Orders: All orders placed by companies and permits not included in the shipping cost, that
or individuals outside the United States require pre- may result from importing products. The customer
payment for their purchase. For your convenience we assumes full responsibility for the shipment after it
do accept pre-payment by international wire. Contact leaves PhytoTechnology Laboratories. If you require
sales@phytotechlab.com for more information. any special documentation before we ship your order,
it is the customer's responsibility to contact customer
High Value Orders: For orders of high value, pre- service before their order has shipped.
payment in full may be required for purchase. Orders
are considered to be high value solely at the discretion PhytoTechnology Laboratories products are available
of PhytoTechnology Laboratories. to any country that enjoys unrestricted trade with the
United States. At the printing of this catalogue, the
only countries that we are unable to ship to are Cuba,
Term Payment and Credit Iran, North Korea, Sudan, and Syria.
For those customers who desire term payment
accounts (Net 30-Day terms), a credit application
A list of our International Distributors can be found on
may be filled out and submitted for approval. We will
the inside back cover.
attempt to approve this credit as soon as possible, but
it is subject to the response time from the customers
trade references. Orders to the European Union
PhytoTechnology Laboratories is pleased to announce
For those customers who qualify for Net 30-Day direct distribution through France. Orders to the
terms, payment will be required within 30 days. EU will be shipped duty free (with a customer VAT
Delinquent accounts will be contacted first with a Number) and will typically arrive within 3 to 4 days.
Past-Due Notice via e-mail and a fax or phone call. For more information contact:
Any orders from customers with past-due balances sales@phytotechlab.eu.com
will be held until such balances are paid in full. At or visit: www.phytotechlab.eu.com.
60 days past due, accounts will be turned over for
Page 8 2015 PhytoTechnology Laboratories
www. p h y t o t e c h l a b .c o m
Shipping Information member of the PhytoTechnology Laboratories
customer service department.
Most products are shipped within 1 to 2 business days
You can choose to have your order billed to your
of receipt of order. If any items in the order are back-
ordered you will be informed via phone or e-mail when personal Federal Express account number. A
the order is placed. Unless otherwise specified by the $5.00 USD per box handling fee will be applied
customer, all back-ordered items will ship separately, to your order.
allowing any in-stock items to ship immediately. Shipments to residential addresses typically incur
Please note the following: additional shipping costs from FedEx.
No orders are shipped on Fridays.
Orders placed within 1 to 2 days of holidays Federal Express Shipping Options
observed by PhytoTechnology Laboratories may
be shipped after the holiday. PhytoTechnology Laboratories provides shipping
through Federal Express (FedEx), which offers
domestic and international shipping. For your
Recommended Shipping Methods convenience we allow the following shipping
To ensure the long-term stability of your items
methods, organized below in increasing speed of
and that they arrive in optimal conditions,
delivery and cost:
PhytoTechnology Laboratories recommends the
Domestic Services

General Information
following:
FedEx Ground Service (See "FedEx Ground
Products intended for room temperature storage
can ship by any method, Service Map" on page 10)
Products intended for 2 to 6 C storage should be FedEx 2 Day Service
shipped by two-day express service or faster, and FedEx Standard Overnight (delivery by 3:00 p.m.
Products that require storage in -20 to 0 C the next day)
storage should be shipped overnight. FedEx Priority Overnight (Delivery by 10:00 a.m.
For orders requiring shipment on dry ice, see the next day; not available in some areas)
"Special Shipping Requirements" on page 11. International Services
FedEx International Ground Service (Canada
While refrigerator storage (at 2 to 6 C) or freezer only)
storage (at -20 to 0 C) is recommended for the FedEx International Economy Service
long-term stability of products marked with specific FedEx International Priority Service
temperature storage ranges, up to seven days in air Other Services
transit at ambient temperatures will not detrimentally FedEx Freight Service
affect the products quality or shelf-life. FedEx Hazardous Shipping Service (domestic,
ground service shipping only)
Shipping Charges FedEx Smart Post
PhytoTechnology Laboratories makes every effort to
provide you with cost-effective shipping solutions. Shipping Quotes
Shipping fees are calculated based solely on the PhytoTechnology Laboratories can, on request,
size and weight of the items being shipped and the provide shipping quotes for international or high
distance being travelled. Please be advised of the volume shipments. You will be provided with an
following: itemized quote, including freight charges that will
For all orders, a $5.00 USD per box handling fee be valid for 30 days only. Please be cognizant that
is included in the shipping cost. shipping prices can fluctuate over time and as such we
Orders cannot be shipped to a PO Box cannot provide shipping quotes that last longer than
maintained by the United States Postal Service 30 days.
(USPS) without prior arrangements having been
made with PhytoTechnology Laboratories.
For large-volume orders we recommend shipping
by FedEx freight. Should your order qualify
for freight shipment you will be informed by a

General Information Page 9


Product Catalogue & Laboratory Guide
FedEx Ground Service Map
PhytoTechnology Laboratories makes every effort to ship products by the safest, most economical method
possible. Products are shipped via traceable courier, unless the customer specifies an alternative method of
shipping. Most products can be shipped anywhere within the continental United States via FedEx Ground
within 3 days (see map below, current as of June 2013).
General Information

Please Remember:
A business day does not include the initial ship date of the order, Saturdays, Sundays, or holidays.
Example: A package is shipped on Monday that takes 2 business days to arrive. The day the package
is shipped does not count as a business day, so your package would arrive on Wednesday, the second
business day after the ship date.
The delivery schedule illustrated above is accurate at the time of this catalog printing; however, it is subject
to change at the carriers discretion.
Keep in mind that shipping rates may change at any time without prior notice. Shipping quotes are valid
only for 30 days from the date of the quote. Shipping charges are determined by weight and zone.
Shipping rates may also be affected by the following:
A minimum $5.00 USD handling charge per box will be added to shipping charges. A surcharge may be
added to shipments to residential addresses by the carrier.
Hazardous material surcharges range from $35.00 USD per Hazardous UN Number for ground
shipments to $75.00 USD per Hazardous UN Number for air shipments.
Box size may affect rates on air shipments.
Products requiring shipping on Dry Ice or Blue Ice will incur a surcharge of $15.00 for domestic
shipments and $35.00 USD for international shipments.
Shipping charge quotes can be provided for domestic orders over $2500 and all international orders. Please
bear in mind that these quotes are estimates and that the actual shipping charges are subject to change.

Page 10 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
Special Shipping Requirements Catalogue Symbols
Hazardous Items Harmful: substances which can have limited
The International Air Transport Association (IATA), effects on health and/or substances which can cause
inflammation and/or irritation.
The International Civil Aviation Organization
(ICAO), and the United States Department of Health Hazard: substances which can cause serious
and/or extremely serious acute, carcinogenic or
Transportation (through Federal Regulation 49
chronic effects on health.
CFR) classify certain products as hazardous products.
Packaging and shipment of these products is Toxic: substances which can be fatal if ingested,
inhaled, or handled without appropriate personal
regulated and all carriers impose Hazardous Material protective equipment.
(HAZMAT) fees. [A minimum $35.00 USD charge
Corrosive: substances which can destroy living
is added for all HAZMAT ground shipments. For tissue. Can cause severe skin burns or eye damage.
shipping hazardous items by air or internationally a May be corrosive to metals.
minimum $75.00 USD fee applies.]
Oxidizing: substances which can produce and/or
intensify high exothermic reactions in contact with
Temperature Sensitive Items other substances.
Products labeled for storage at -20 to 0 C or lower Flammable: substances with a flash point greater

General Information
will be shipped on Dry Ice or Blue Ice as needed than 21C and less than or equal to 55C. Keep
to ensure product stability. Dry Ice and Blue Ice away from heat, sparks, hot surfaces and open flame.
shipments require an additional fee of $15.00 USD Dangerous for the Environment: substances which
minimum. For international shipments requiring Dry can cause serious effects on the environment.
Ice or Blue Ice a fee of $35.00 USD will be charged
(Prices are subject to change without notice). Hazardous: substances deemed generally hazardous.
Hazardous shipping fees apply to these products.

Liquid Solution: product is a liquid solution or is


naturally a liquid product. Individual product listings
will list liquid type (e.g., water or PBS Buffer).
Product is shipped on Dry or Blue Ice: to preserve
product stability, dry or blue ice shipping is
required. Dry or blue ice shipping fees apply.

General Information Page 11


Product Catalogue & Laboratory Guide
Custom Services PhytoTechnology Laboratories can currently
package Liquid Media in sizes ranging from 10mL
Custom Dry Powder Media bottles to 20L bags.
PhytoTechnology Laboratories can custom
manufacture proprietary and non-proprietary dry Custom Packaging
powder media for customers according to cGMP PhytoTechnology Laboratories can custom package
standards, and in accordance with ISO standards proprietary and non-proprietary dry powder media
of quality, in an environmentally controlled for customers according to cGMP standards in
manufacturing facility in Overland Park, KS. their environmentally controlled packaging rooms.
This service has proven to save time and money in PhytoTechnology Laboratories currently maintains
media preparation: multiple environmentally controlled packaging and
Custom media reduces the possibility of formulation rooms. With rooms dedicated to the
technician error in the formulation of media; packaging of either Non-Animal Derived Products,
In many cases, a single lot of a custom medium Animal Derived Products, or Antibiotics, ensuring no
can be made to meet your annual research or cross-contamination between product types.
production requirements, ensuring consistency Media can be packaged in foil pouches or bottles
in the medium throughout a research program or in the amounts required by the end-user;
General Information

production cycle; Custom media packaging in ready-to-use sizes


Custom dry powder media can be manufactured reduces the possibility of technician error in the
in batch sizes ranging from 100L to 50,000L, preparation of the media; this helps to ensure the
depending upon the formulation's complexity. accuracy and batch-to- batch consistency of media
Custom dry powder media can be formulated prepared by in-house technicians;
to contain any or all of the following: basal salts, Biochemicals or other media components such
carbohydrates, plant growth regulators, gelling as carbohydrates or gelling agents can be custom
agents, and can be buffered and pH adjusted. packaged in ready-to-use packages. This eliminates
A mutually signed confidentiality agreement customers spending time weighing out chemicals,
provides assurance that all records pertaining to thus improving their efficiency;
a custom medium will be held in the strictest These services have proven to save time and
confidence. money in media preparation for customers.
PhytoTechnology Laboratories is currently
providing this service for a number of agro-
biotech firms, micropropagation labs, and bio-
Custom Testing
PhytoTechnology Laboratories can offer custom
pharmaceutical companies.
testing services at an additional charge. These testing
services are in addition to the normal testing of media
Custom Liquids conducted by the company. These services are fee-
The supply of liquid media offers scientists and based and include, but are not limited to:
production personnel sterile, ready-to-use media ICP Elemental Analysis
for bioreactors, micropropagation production, and CHN Analysis
research. SOPs specific for each phase of liquid USP Sterility Testing
media formulation, manufacturing and packaging are Bioburden Testing
stringently followed. PhytoTechnology Laboratories can, in
All liquid media products manufactured by conjunction with the researcher, develop and/or
PhytoTechnology Laboratories are sterility- optimize proprietary media and test to meet the
tested according to US Pharmacopeia (USP) requirements for any specific plant cell line
specifications; provided by the customer.
Custom media reduces the possibility of
technician error in the formulation of the media;
Custom liquid media can be manufactured in
batch sizes ranging from 10L to 200L depending
upon the complexity of the media;
Page 12 2015 PhytoTechnology Laboratories
www. p h y t o t e c h l a b .c o m
Custom Orders Process Use of Our Products
To start a custom order process either visit
All products sold by PhytoTechnology Laboratories
www.phytotechlab.com, contact us by e-mail at
are intended for laboratory and research use only, and
tech@phytotechlab.com or contact us directly at
are not to be used on humans or animals, or for the
913.341.5343 and ask for a custom order form.
production of food supplements, drugs, or cosmetics.
1. Fill out the custom order form. Making sure to fill
United States government regulations require us
out:
to exercise due diligence in screening all orders and
Your contact information,
we are further expected to refuse to ship any order
The desired formulation of your custom
where improper use is indicated or suspected. Thus
media,
PhytoTechnology Laboratories reserves the right to
The batch and package sizes required, or
refuse service to anyone suspected of misusing these
The custom testing services required (if
products.
applicable).
2. Return the form to tech@phytotechlab.com or
Our plant growth regulators and media containing
by faxing it to 913.341.5442.
plant growth regulators are intended only for
3. Indicate if a signed confidentiality agreement is
laboratory use, plant tissue culture media preparation,
required.

General Information
plant micropropagation, and plant research purposes.
4. Custom orders usually take about a week to
prepare a quote. We look to ensure and confirm:
Products offered by PhytoTechnology Laboratories
Correct formulation,
are intended for use by qualified professionals who
Feasibility of manufacturing,
are familiar with their potential hazards and trained
Availability of biochemical components,
in safe laboratory practices. Information pertaining
Pricing of the order, and
to the potential hazards of our products is provided
When the order can be manufactured.
on our product labels and in our Material Safety Data
5. After the order has been reviewed and confirmed
Sheets.
by both parties, manufacturing can generally
occur within 1 to 3 business weeks.
Material Safety Data Sheets are available online at
6. The order will be physio-chemically tested to
www.phytotechlab.com, as required by OSHAs
ensure that it was manufactured correctly.
Hazard Communication Standard, for products that
7. Packaging of the order usually occurs within 24 to
are hazardous. The absence of a warning must not be
48 hours after passing physio-chemical testing.
interpreted as an indication of safety. The safe use of
8. The order ships, to most locations in the United
each product must be determined by the end user.
States, within 3 business days.

For custom testing, turnaround can be one to four Safety Documentation


weeks depending on the testing services desired. PhytoTechnology Laboratories, in accordance
with ISO quality guidelines and local and federal
Custom Media quotation forms can now be filled regulations provides the following documentation for
out entirely online at www.phytotechlab.com. all products, as applicable:
Material Safety Data Sheets: Also known
as MSDS(s), a Material Safety Data Sheet is
available for all chemicals sold and manufactured
by PhytoTechnology Laboratories. These sheets
include information on any potential hazards
of the chemical, proper handling and storage
recommendations, and shipping and regulatory
information.

General Information Page 13


Product Catalogue & Laboratory Guide
Certificates of Analysis: Also known as CofA(s)
and COA(s), Certificates of Analysis provide
Refund & Restocking Policies
in-depth chemical and stability analysis of every The customer service department at PhytoTechnology
chemical on a lot-to-lot basis. Each Certificate Laboratories is available to assist you, should a
of Analysis covers only one manufacturing problem arise. Please inspect your packages upon
or packaging lot of a chemical, and specific receipt and notify us immediately of any damage
information such as expected shelf-life can vary that may have occurred with any orders. Prior to
slightly from lot to lot. returning any product, please contact our customer
Product Information Sheets: Available for service department to obtain authorization and proper
many PhytoTech products, and are intended to shipping procedures. PhytoTechnology Laboratories
provide information about a product's typical will do its best to accommodate your request to return
usage, applicable plant cell lines, and references products. However, to maintain the quality of our
to literature on the product. Product Information products, certain products may not be accepted for
Sheets also contain media formulation tables for return. Sterile products, hazardous products, and
all non-proprietary media formulations. products which require refrigerated or freezer storage
may not be returned. There will be a 15% restocking
All of these documents are available at fee applied to the return of any product. Returns must
General Information

www.phytotechlab.com or can be obtained by be made within 30 days of purchase.


contacting tech@phytotechlab.com.

Warranties
PhytoTechnology Laboratories warrants that its
products perform as described in PhytoTechnology
Laboratories literature. The purchaser must
determine the suitability of the products for his/her
particular use. Should any product fail for reasons
other than misuse, PhytoTechnology Laboratories
will replace the product free of charge or refund the
purchase price, at the company's discretion.

This warranty is exclusive, and PhytoTechnology


Laboratories makes no other warranty, expressed
or implied, including any implied warranty of
merchantability of fitness for any particular purpose.

PhytoTechnology Laboratories shall not be liable for


any incidental, consequential, or contingent damages.

Page 14 2015 PhytoTechnology Laboratories


Product Catalogue & Laboratory Guide
Biochemicals Featured in this section
PhytoTechnology Laboratories provides
Agars
an extensive line of biochemicals for use
in plant tissue culture, microbiology, and
molecular biology research. This section
Amino Acids
outlines many of these products. Most
of the products listed in this section have Auxins
a wide variety of uses. Products that are
intended for, or used most commonly
with one specific field of plant sciences are Buffers
included in those sections. Please refer to
the following sections:
Molecular Biology, page 131 Carbohydrates
Microbiology, page 151
Seed Testing, page 165
Phycology, page 173 Carrageenans
For assistance with locating products
within the catalogue, please consult the
Cytokinins
Biochemicals

indices at the back of this catalogue.


Be sure to check back at Enzymes
www.phytotechlab.com for new products
often.
Gellan Gums
Gelling Agents
PhytoTechnology Laboratories offers
several different types of gelling agents for Gibberellins
use in plant tissue culture, including agars,
gellan gums, and carrageenans. Macronutrients
Agars:
A111 - Agar, Gracilaria
A296 - Agar, Gelidium Micronutrients
A175 - Agar TC, Purified Grade
Gellan Gums:
G434 - Gellan Gum pH Adjustment
G3251 - Gelzan
Carrageenans:
C257 - Carrageenan, Gelcarin Reagents
C2000 - Carrageenan, High Clarity
Solvents
Media Components
Most of the biochemical components used
in the manufacture of PhytoTechnology Vitamins
Laboratories tissue culture media are
available for purchase individually. This
includes a wide variety of plant growth
regulators, vitamins, amino acids,
carbohydrates, and undefined organic
supplements.
Page 16 2015 PhytoTechnology Laboratories
www. p h y t o t e c h l a b .c o m
A102 - (+/-)-Abscisic Acid A120 - Adenine
ABA; Dormin; Abscisin II; (2Z,4E)-5-[(1S)-Hydroxy-2,6,6- 6-Aminopurine; Vitamin B4
trimethyl-4-oxo-2-cyclohexen-1-yl]-3-methyl-2,4-pentadienoic Plant Tissue Culture Tested
Acid Powder
Abscission-accelerating plant hormone CAS Number: 73-24-5
Plant Tissue Culture Tested Formula: C5H5N5
Powder Molecular Weight: 135.13
CAS Number: 14375-45-2 Soluble in KOH
Formula: C15H20O4 Store at Room Temperature
Molecular Weight: 264.32 Merck 13, 152
Soluble in KOH Available Package Sizes
Store at -20 to 0 C
Merck 13, 9 25g 100g

Available Package Sizes A545 - Adenine Hemisulfate


100mg 500mg 1g 5g 6-Aminopurine Sulfate
Plant Tissue Culture Tested
A256 - Acetic Acid, Glacial
Powder
Ethanoic Acid CAS Number: 321-30-2
Liquid Formula: (C5H6N5)2SO4
CAS Number: 64-19-7 Molecular Weight: 368.34
Formula: C2H4O2 Soluble in Warm Water
Molecular Weight: 60.05 Store at Room Temperature

Biochemicals
Miscible with Water Merck 13, 152
Store at Room Temperature Available Package Sizes
Merck 13, 56
25g 100g

A296 - Agar, Plant TC


Micropropagation Grade - Gelidium
Agar-Agar; Gum Agar
Gel Strength: minimum 700 g/cm2
Available Package Sizes
Plant Tissue Culture Tested
10x1mL 10mL 25mL
Powder
A104 - Acetosyringone CAS Number: 9002-18-0
Soluble in Boiling Water
See Molecular Biology Section for Complete Listing Store at Room Temperature
Merck 13, 184
A110 - Acetosyringone 100mM Solution
See Molecular Biology Section for Complete Listing Available Package Sizes
9g 100g 500g 1Kg 5Kg 10Kg 25Kg
A118 - Acetylsalicylic Acid
2-(Acetyloxy)benzoic Acid; Salicilic acid acetate; Aspirin A111 - Agar, Plant TC
Plant Tissue Culture Tested Micropropagation Grade - Gracilaria
Powder Agar-Agar; Gum Agar
CAS Number: 50-78-2 Gel Strength: minimum 900 g/cm2
Formula: C9H8O4 Plant Tissue Culture Tested
Molecular Weight: 180.16 Powder
Soluble in Hot to Boiling Water or DMSO CAS Number: 9002-18-0
Store at Room Temperature Soluble in Boiling Water
Merck 13, 856 Store at Room Temperature
Available Package Sizes Merck 13, 184
100g Available Package Sizes
9g 100g 500g 1Kg 5Kg 10Kg 25Kg

Biochemicals A-Z Page 17


Product Catalogue & Laboratory Guide
Gelling Agents Usage Chart A1315 - Agarose, Low Gelling Temp, SeaPlaque
See "Figure 19. Usage Guide for Gelling Agents" on page 246 Gelling Temperature: 26 to 30 C
in the Technical Section. Gelling Strength (1% concentration): 200 g/cm2
Plant Tissue Culture Tested
A175 - Agar, TC; Purified Grade - Gelidium Powder
Agar-Agar; Gum Agar Low EEO
Gel Strength: minimum 700 g/cm2 CAS Number: 9012-36-6
Plant Tissue Culture Tested Formula: (C12H14O5(OH)4)n
Powder Molecular Weight: Variable
CAS Number: 9002-18-0 Soluble in Hot Water
Soluble in Boiling Water Store at Room Temperature
Store at Room Temperature Available Package Sizes
Merck 13, 184
25g
Available Package Sizes
8g 100g 250g 500g 1Kg 2.5Kg 5Kg A121 - L-Alanine
USP Grade
A133 - Agargellan (S)-2-Aminopropanoic Acid
A proprietary blend of Gellan Gum and Micropropagation Plant Tissue Culture Tested
grade Agar. Powder
Plant Tissue Culture Tested CAS Number: 56-41-7
Powder Formula: C3H7NO2
Soluble in Boiling Water Molecular Weight: 89.09
Biochemicals

Store at Room Temperature Soluble in Water


Store at Room Temperature
Available Package Sizes Merck 13, 203
100g 500g 1Kg 5Kg
Available Package Sizes
A110 - Agarose 100g 500g 1Kg
Gelling Temperature: >35C
Plant Tissue Culture Tested A1286 - Alcian Blue
Powder See Seed Testing Section for Complete Listing
CAS Number: 9012-36-6
A108 - Alginic Acid, Sodium Salt
Formula: (C12H14O5(OH)4)n
Low EEO Sodium Alginate
Molecular Weight: Variable Powder
Soluble in Hot Water CAS Number: 9005-38-3
Store at Room Temperature Molecular Weight: 240,000 avg.
Partially Soluble in Water Forms Viscous Colloidal
Available Package Sizes
Suspension
100g Store at Room Temperature
Merck 13, 238
A1050 - LE Agarose
See Molecular Biology Section for Complete Listing Available Package Sizes
100g 250g 500g
A105 - Agarose, Low Gelling Temperature
Gelling Temperature: 26 to 29 C A127 - Aluminum Chloride Hexahydrate
Plant Tissue Culture Tested Plant Tissue Culture Tested
Powder Powder
CAS Number: 9012-36-6 CAS Number: 7784-13-6
Formula: (C12H14O5(OH)4)n Formula: AlCl36H2O
Molecular Weight: Variable Molecular Weight: 241.43
Soluble in Hot Water Soluble in Water
Store at Room Temperature Store at Room Temperature
Merck 13, 335
Available Package Sizes
100g Available Package Sizes
100g

Page 18 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
A103 - p-Aminobenzoic Acid New Product
4-Aminobenzoic Acid; PABA; Vitamin BX; Vitamin H1 A1180 - 1-Aminocyclopropanecarboxylic Acid
Plant Tissue Culture Tested
ACC, ACPC, 1-Aminocyclopropane-1-carboxylic Acid
Powder
CAS Number: 150-13-0 ACC is a cyclic -amino acid that is a precursor of the plant
Formula: C7H7NO2 hormone ethylene.
Molecular Weight: 137.14 Plant Tissue Culture Tested
Soluble in KOH/NaOH
Powder
Slightly soluble in water at low concentrations
CAS Number: 22059-21-8
Store at 2 to 6 C
Formula: C4H7NO2
Merck 13, 422
Molecular Weight: 101.10
Available Package Sizes Soluble in Water/DMSO
5g 25g 100g Store at Room Temperature
Available Package Sizes
A109 - Ammonium Chloride
100mg 500mg 1g
Plant Tissue Culture Tested
Powder A112 - Ammonium Molybdate (VI) Tetrahydrate
CAS Number: 12125-02-9 Molybdic Acid, Ammonium Salt Tetrahydrate
Formula: NH4Cl Plant Tissue Culture Tested
Molecular Weight: 53.49
Powder
Soluble in Water
CAS Number: 12027-67-7
Store at Room Temperature

Biochemicals
Formula: (NH4)6Mo7O244H2O
Merck 13, 510
Molecular Weight: 1235.9
Available Package Sizes Soluble in Water
100g 1Kg Store at Room Temperature
Merck 13, 536
A114 - Ammonium Citrate, Dibasic Available Package Sizes
Diammonium Citrate; Citric Acid Diammonium Salt 25g 100g 500g 100g
Plant Tissue Culture Tested
Powder Macronutriens Sources Chart
CAS Number: 3012-65-5 See "Figure 9. List of Macronutrient Sources & PhytoTech
Formula: (NH4)2HC6H5O7 Product Numbers" on page 236 in the Technical Section.
Molecular Weight: 226.19
Soluble in Water A300 - Ammonium Nitrate
Store at Room Temperature Plant Tissue Culture Tested
Merck 13, 513
Powder
Available Package Sizes CAS Number: 6484-52-2
500g 1Kg Formula: NH4NO3
Molecular Weight: 80.04
Soluble in Water
Store at Room Temperature
Merck 13, 538
Available Package Sizes
500g 1Kg 5Kg 10Kg 15Kg

Biochemicals A-Z Page 19


Product Catalogue & Laboratory Guide
A113 - Ammonium Phosphate, Monobasic A126 - Antioxidant Mixture
Ammonium Dihydrogen Phosphate A mixture of Ascorbic Acid and Citric Acid
Plant Tissue Culture Tested Plant Tissue Culture Tested
Powder Use 0.25 grams per liter to make a stock solution.
CAS Number: 7722-76-1 This product is typically made up as a stock solution, and an
Formula: NH4H2PO4 explant is dipped into it before transferred to fresh medium.
Molecular Weight: 115.03 It may also be added directly to the medium in amounts
Soluble in Water determined by the user.
Store at Room Temperature Powder
Merck 13, 547 Formula: C6H8O6/ C6H8O7
Available Package Sizes Molecular Weight: 176.13/ 192.14
Soluble in Water
100g 500g 1Kg
Store at Room Temperature
A305 - Ammonium Sulfate Available Package Sizes
Sulfuric Acid Diammonium Salt 1L 10L
Plant Tissue Culture Tested
Powder A124 - Apple Powder
CAS Number: 7783-20-2 A mixture of natural apple puree and maltodextrin.
Formula: (NH4)2SO4 Plant Tissue Culture Tested
Molecular Weight: 132.14 Powder
Soluble in Water Soluble in Water
Store at Room Temperature Store at Room Temperature
Biochemicals

Merck 13, 559


Available Package Sizes
Available Package Sizes
500g 1Kg
500g 1Kg
A143 - L-Arginine, Free Base
A116 - Ampicillin, Sodium Salt
(S)-2-Amino-5-[(aminoiminomethyl)amino]pentanoic Acid
See Molecular Biology Section for Complete Listing Plant Tissue Culture Tested
A1116 - Ampicillin Solution (100mg/mL) Powder
CAS Number: 74-79-3
See Molecular Biology Section for Complete Listing
Formula: C6H14N4O2
A123 - Ancymidol Molecular Weight: 174.20
Soluble in Water
-Cyclopropyl--[4-methoxyphenyl]-5-pyrimidinemethanol Store at Room Temperature
Plant Tissue Culture Tested Merck 13, 785
Powder
Available Package Sizes
CAS Number: 12771-68-5
Formula: C15H16N2O2 100g 500g 1Kg
Molecular Weight: 256.3
Soluble in DMSO
A106 - L-Ascorbic Acid
Store at 2 to 6 C 3-Oxo-L-gulofuranolactone; Vitamin C
Merck 13, 637 Plant Tissue Culture Tested
Available Package Sizes Powder
CAS Number: 50-81-7
100mg 500mg 1g
Formula: C6H8O6
A1272 - Aniline Blue Molecular Weight: 176.13
Soluble in Water
See Seed Testing Section for Complete Listing Store at Room Temperature
Merck 13, 837
Available Package Sizes
100g 500g 1Kg

Page 20 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
A107 - L-Asparagine B852 - Banana Powder
(S)-2,4-Diamino-4-oxobutanoic Acid Monohydrate; A mixture of natural banana puree and maltodextrin.
-Aminosuccinamic Acid Monohydrate Plant Tissue Culture Tested
Plant Tissue Culture Tested Powder
Powder Suspension in Water
CAS Number: 5794-13-8 Store at Room Temperature
Formula: C4H8N2O3H2O Available Package Sizes
Molecular Weight: 150.1
Soluble in Water 30g 100g 500g 1Kg
Store at Room Temperature
Merck 13, 842
B151 - N6-Benzoyladenine
Plant Tissue Culture Tested
Available Package Sizes
Powder
25g 100g 500g 1Kg
CAS Number: 4005-49-6
A117 - L-Aspartic Acid, Free Acid Formula: C12H9N5O
Molecular Weight: 239.2
(S)-Aminobutanedioic Acid; Aminosuccinic Acid Soluble in DMSO
Plant Tissue Culture Tested Store at 2 to 6 C
Powder
Available Package Sizes
CAS Number: 56-84-8
Formula: C4H7NO4 1g 25g
Molecular Weight: 133.1
Soluble in Water
Making Stock Solutions of Plant Growth

Biochemicals
Store at Room Temperature Regulators
Merck 13, 845 See "Figure 16. Stock Solution Dilution Chart" on page 242
Available Package Sizes in the Technical Section.
100g 500g 1Kg B800 - 6-Benzylaminopurine
A147 - Auxindole BA; N6-Benzyladenine
N-(phenylmethyl)-1H-purin-6-amine
3-Bromooxindole-3-acetic Acid Plant Tissue Culture Tested
Plant Tissue Culture Tested
Powder
Auxindole is the trade name of 3-bromooxindole-3-acetic CAS Number: 1214-39-7
acid, which is instantaneously and quantitatively converted to Formula: C12H11N5
3-methyleneoxindole [(MO);(FW = 145)] in aqueous solutions. Molecular Weight: 225.3
MO polymerizes readily at concentrations 1 mM; stock Soluble in KOH
solutions should be diluted as soon as possible. Store at Room Temperature

MO is a potent sulfhydryl reagent that forms adducts Available Package Sizes


with sulfhydryl (-SH) compounds and sulfur-containing 1g 5g 25g 100g
nucleophiles; therefore, such compounds should not be used as
solvents or buffer components. The use of dimethyl sulfoxide B130 - 6-Benzylaminopurine Solution (1 mg/mL)
(DMSO) as a solvent is particularly discouraged. BA; N6-Benzyladenine
MO is sensitive to pH extremes. The biological activity of MO N-(phenylmethyl)-1H-purin-6-amine
is best demonstrated between pH 5.7 and 6.8. Sterile Filtered
Plant Tissue Culture Tested
For instructions on how to use this product, review the product
Liquid
information available on our website.
CAS Number: 1214-39-7
Powder Formula: C12H11N5
Formula: C10H8BrNO3 Molecular Weight: 225.3
Molecular Weight: 269.9 Miscible with Water
Soluble in EtOH Store at 2 to 6 C
Store at -20 to 0 C
Available Package Sizes
Available Package Sizes
100mL 500mL 1L
10mg 100mg
X884 - Blue-Gal
See Molecular Biology Section for Complete Listing

Biochemicals A-Z Page 21


Product Catalogue & Laboratory Guide
B140 - D-Biotin New Product
Hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanoic B1376 - Bromocresol Purple, Sodium Salt
Acid; Vitamin H; Coenzyme R 5,5-Dibromo-o-cresolsulfonephthalein; BCP
Plant Tissue Culture Tested
Powder A pH indicator that is yellow below pH 5.2 and purple above
CAS Number: 58-85-5 pH 6.8. It can be used as a fluorescent stain to detect yeast cells
Formula: C10H16N2O3S with plasma membrane damage for dead cell counts.
Molecular Weight: 244.31 Powder
Soluble in Warm Water or KOH CAS Number: 62625-30-3
Store at Room Temperature Formula: C21H15Br2NaO5S
Merck 13, 1231 Molecular Weight: 562.20
Available Package Sizes Soluble in Water
1g 5g Store at Room Temperature
Merck 13, 1371
B210 - Boric Acid Available Package Sizes
Plant Tissue Culture Tested 5g 25g
Powder
CAS Number: 10043-35-3 B1714 - Bromoyhymol Blue
Formula: H3BO3 See Seed Testing Section for Complete Listing
Molecular Weight: 61.83
Soluble in Water B148 - 4-Bromophenoxyacetic Acid
Store at Room Temperature Plant Tissue Culture Tested
Biochemicals

Merck 13, 1326


Powder
Available Package Sizes CAS Number: 1878-91-7
500g 1Kg Formula: C8H7BrO3
Molecular Weight: 231.05
B619 - Boric Acid, USP Grade Soluble in KOH
Plant Tissue Culture Tested Store at Room Temperature
Powder Available Package Sizes
CAS Number: 10043-35-3 25g
Formula: H3BO3
Molecular Weight: 61.83 B155 - Bromoxynil
Soluble in Water 3,5-Dibromo-4-hydroxybenzonitrile
Store at Room Temperature
Powder
Merck 13, 1326
CAS Number: 1689-84-5
Available Package Sizes Formula: C7H3Br2NO
500g 1Kg Molecular Weight: 276.92
Soluble in DMSO
B131 - Bialaphos Store at Room Temperature
See Molecular Biology Section for Complete Listing Merck 13, 1426
Available Package Sizes
B1730 - Bialaphos Solution (1mg/mL)
100mg 250mg
See Molecular Biology Section for Complete Listing

B162 - BIS-TRIS B235 - Buffer Solution, pH Reference Standard,


pH 4.0 0.02 at 25 C
See Molecular Biology Section for Complete Listing
Color Coded Red
B441 - Bradford Reagent Used for pH meter calibration
See Molecular Biology Section for Complete Listing Liquid
Store at Room Temperature

Available Package Sizes


500mL 4L

Page 22 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
B236 - Buffer Solution, pH Reference Standard, C625 - Calcium Chloride Ahydrous, USP Grade
pH 7.0 0.02 at 25 C USP Grade
Color Coded Yellow Plant Tissue Culture Tested
Used for pH meter calibration Powder
CAS Number: 10043-52-4
Liquid
Formula: CaCl2
Store at Room Temperature
Molecular Weight: 110.98
Soluble in Water
Available Package Sizes Store at Room Temperature
500mL 4L Merck 13, 1660
Available Package Sizes
B237 - Buffer Solution, pH Reference Standard,
500g 1Kg
pH 10.0 0.02 at 25 C
Color Coded Blue C231 - Calcium Gluconate Monohydrate
Used for pH meter calibration D-Gluconic Acid Calcium Salt Monohydrate
Liquid Plant Tissue Culture Tested
Store at Room Temperature Powder
CAS Number: 299-28-5
Formula: C12H22O14CaH2O
Available Package Sizes Molecular Weight: 448.39
500mL 4L Soluble in Hot Water
Store at Room Temperature

Biochemicals
C135 - Calcium Chloride Dihydrate Merck 13, 1671
Plant Tissue Culture Tested Available Package Sizes
Powder 500g 1Kg
CAS Number: 10035-04-8
Formula: CaCl22H2O C180 - Calcium Nitrate
Molecular Weight: 147.02 Plant Tissue Culture Tested
Soluble in Water
Store at Room Temperature Powder
Merck 13, 1660 CAS Number: 13477-34-4
Formula: Ca(NO3)24H2O
Available Package Sizes Molecular Weight: 236.15
500g 1Kg 5Kg Soluble in Water
Store at Room Temperature
C266 - Calcium Chloride Anhydrous Merck 13, 1687
FCC Grade Available Package Sizes
Plant Tissue Culture Tested
500g 1Kg 5Kg
Powder
CAS Number: 10043-52-4 C186 - Calcium Pantothenate
Formula: CaCl2 USP Grade
Molecular Weight: 110.98 N-[(2R)-2,4-Dihydroxy-3,3-dimethyl-1-oxobutyl]--alanine,
Soluble in Water Calcium Salt; D-Pantothenic Acid, Calcium Salt;
Store at Room Temperature Vitamin B5
Merck 13, 1660 Plant Tissue Culture Tested
Available Package Sizes Powder
500g 1Kg 5Kg CAS Number: 137-08-6
Formula: C18H32O10N2Ca
Molecular Weight: 476.54
Soluble in Water
Store at 2 to 6 C
Merck 13, 7085
Available Package Sizes
100g 500g 1Kg

Biochemicals A-Z Page 23


Product Catalogue & Laboratory Guide
C274 - Calcium Phosphate, Tribasic C257 - Carrageenan
Calcium Hydroxyphosphate Gelcarin GP 812; Irish Moss; Kappa-Type Carrageenan
Plant Tissue Culture Tested Plant Tissue Culture Tested
Powder Gelcarin GP 812 is a registered trademark of FMC
CAS Number: 12167-74-7 BioPolymer.
Formula: Ca5(OH) (PO4)3 Powder
Molecular Weight: 502.31 CAS Number: 9000-07-1
Soluble in HCl Soluble in Boiling Water
Store at Room Temperature Store at Room Temperature
Merck 13, 1878
Available Package Sizes
100g 500g Available Package Sizes
250g 1Kg 5Kg 25Kg
C134 - Calcium Silicate
Calcium Metacilicate; Calcium Silicon oxide C2000 - Carrageenan, High Clarity
Plant Tissue Culture Tested Irish Moss; Kappa-Type Carrageenan
Powder Plant Tissue Culture Tested
CAS Number: 1344-95-2 Powder
Formula: CaSiO3 CAS Number: 9000-07-1
Molecular Weight: 116.16 Soluble in Boiling Water
Suspension in Water Store at Room Temperature
Store at Room Temperature Merck 13, 1878
Merck 13, 1707 Available Package Sizes
Biochemicals

Available Package Sizes 10g 100g 500g 1Kg 5Kg 10Kg


25g 1Kg
C184 - Casein, Enzymatic Hydrolysate
C1933 - Calcofluor White M2R Plant Tissue Culture Tested
Calcofluor White ST; Fluorescent Brightner 28 disodium salt Powder
Calcofluor is a fluorescent stain used to indicate cell wall CAS Number: 9000-71-9
biosynthesis in both plant and fungal species. It can also be Soluble in Water
used as a viability stain. Store at Room Temperature
Powder Available Package Sizes
CAS Number: 4193-55-9
Formula: C40H42N12O10S2Na2 100g 500g 1Kg
Molecular Weight: 960.95
Soluble in Water
C380 - Cefotaxime, Sodium Salt
Store at Room Temperature See Molecular Biology Section for Complete Listing
Available Package Sizes C537 - Cefotaxime Solution (100 mg/mL)
1g 5g 25g See Molecular Biology Section for Complete Listing
C346 - Carbenicillin, Disodium Salt C1880 - Cefotaxime Solution (250 mg/mL)
See Molecular Biology Section for Complete Listing See Molecular Biology Section for Complete Listing
C540 - Carbenicillin Solution (100 mg/mL) C1842 - Cellobiose
See Molecular Biology Section for Complete Listing See Seed Testing Section for Complete Listing
C2046 - Carbenicillin Solution (250 mg/mL) C1970 - Cephalexin
See Molecular Biology Section for Complete Listing See Seed Testing Section for Complete Listing

C2112 - Cephalexin Solution (100mg/mL)


See Seed Testing Section for Complete Listing

Page 24 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
Undefined Organic Supplements Used in Plant V883 - Cholecalciferol
Tissue Culture (3,5Z,7E)-9,10-Secocholesta-5,7,10(19)-trien-3-ol; Vitamin D3
See "Figure 15. List of Common Undefined Organic Plant Tissue Culture Tested
Supplements & PhytoTech Product Numbers" on page 240 Powder
in the Technical Section. CAS Number: 67-97-0
Formula: C27H44O
C325 - Charcoal, Activated Molecular Weight: 384.65
Carbon Black; Activated Carbon; Acid Washed Soluble in DMSO
Plant Tissue Culture Tested Store at 2 to 6 C
Powder Merck 13, 10079
CAS Number: 7440-44-0 Available Package Sizes
Formula: C 1g
Molecular Weight: 12.01
Suspension in Water C232 - Choline Chloride
Store at Room Temperature
2-Hydroxy-N,N,N-trimethylethanaminium Chloride;
Merck 13, 1818
Vitamin B4
Available Package Sizes Plant Tissue Culture Tested
1Kg Powder
CAS Number: 67-48-1
C526 - CHAPS Formula: C5H14ClNO
See Molecular Biology Section for Complete Listing Molecular Weight: 139.63
Soluble in Water

Biochemicals
C207 - Chlormequat Chloride Store at Room Temperature
CCC; Chlorocholine Chloride; Merck 13, 2226
[2-Chloroethyl]trimethylammonium Chloride Available Package Sizes
Plant Tissue Culture Tested
100g 500g
Powder
CAS Number: 999-81-5 C283 - trans-Cinnamic Acid
Formula: C5H13Cl2N (E)-3-Phenyl-2-propenoic Acid
Molecular Weight: 158.07 Plant Tissue Culture Tested
Soluble in Water
Store at Room Temperature Powder
Merck 13, 2120 CAS Number: 140-10-3
Formula: C9H8O2
Available Package Sizes Molecular Weight: 148.16
100mg 1g Soluble in KOH
Store at Room Temperature
C213 - p-Chlorophenoxyacetic Acid Merck 13, 2321
CPA; 4-Chlorophenoxyacetic Acid Available Package Sizes
Plant Tissue Culture Tested
25g
Powder
CAS Number: 122-88-3 C277 - Citric Acid Anhydrous
Formula: C8H7ClO3 2-Hydroxy-1,2,3-propanetricarboxylic Acid
Molecular Weight: 186.59 Plant Tissue Culture Tested
Soluble in KOH
Store at Room Temperature Powder
CAS Number: 77-92-9
Available Package Sizes Formula: C6H8O7
25g 100g Molecular Weight: 192.14
Soluble in Water
Store at Room Temperature
Merck 13, 2350
Available Package Sizes
25g 500g 1Kg

Biochemicals A-Z Page 25


Product Catalogue & Laboratory Guide
C350 - Cobalt Chloride Hexahydrate C195 - Coconut Water
Cobalt(II) Chloride Hexahydrate; Cobaltous Chloride Natural
Hexahydrate Sterile Filtered
Plant Tissue Culture Tested Plant Tissue Culture Tested
Powder Liquid
CAS Number: 7791-13-1 Miscible with Water
Formula: CoCl26H2O Store at 2 to 6 C
Molecular Weight: 237.84
Soluble in Water Available Package Sizes
Store at Room Temperature 100mL 500mL 1L
Merck 13, 2462
C226 - Colchicine
N-[(7S)-5,6,7,9-Tetrahydro-1,2,3,10-tetramethoxy-9-
oxobenzo[a]heptalen-7-yl]acetamide
Available Package Sizes Plant Tissue Culture Tested
25g 100g
Antimitotic commonly used for doubling chromosomes.
C193 - Cobalt Nitrate Hexahydrate Powder
Cobalt(II) Nitrate Hexahydrate; Cobaltous Nitrate CAS Number: 64-86-8
Hexahydrate Formula: C22H25NO6
Plant Tissue Culture Tested Molecular Weight: 399.44
Soluble in Water
Powder Store at Room Temperature
CAS Number: 10026-22-9
Biochemicals

Merck 13, 2496


Formula: Co(NO3)26H2O
Molecular Weight: 291.04
Soluble in Water
Tariff Code: 2834.29.6050
Store at Room Temperature
Merck 13, 2469
Available Package Sizes
500mg 1g 25g
Available Package Sizes
25g 100g New Product
Undefined Organic Supplements Chart C2026 - Colchicine Solution (2.5 mg/mL)
See "Figure 15. List of Common Undefined Organic N-[(7S)-5,6,7,9-Tetrahydro-1,2,3,10-tetramethoxy-9-
Supplements & PhytoTech Product Numbers" on page 240 oxobenzo[a]heptalen-7-yl]acetamide
in the Technical Section. Plant Tissue Culture Tested
Antimitotic commonly used for doubling chromosomes.
C187 - Coconut Powder Liquid
A mixture of natural coconut puree and maltodextrin. CAS Number: 64-86-8
Plant Tissue Culture Tested Formula: C22H25NO6
Powder Molecular Weight: 399.44
Suspension in Water Miscible with Water
Store at Room Temperature Store at 2 to 6 C
Light Sensitive
Available Package Sizes Merck 13, 2496
100g 500g 1Kg

Available Package Sizes


25mL 100mL

Page 26 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
C466 - Copper(II) Sulfate Solution (4%) C242 - Cyanocobalamin
See Molecular Biology Section for Complete Listing USP Grade
5,6-Dimethylbenzimidazolyl Cyanocobalamide; Vitamin B12
C279 - 4-CPPU Plant Tissue Culture Tested
N-(2-Chloro-4-pyridyl)-N-phenylurea Powder
Plant Tissue Culture Tested CAS Number: 68-19-9
Powder Formula: C63H88CoN14O14P
CAS Number: 68157-60-8 Molecular Weight: 1355.36
Formula: C12H10ClN3O Soluble in Water
Molecular Weight: 247.7 Store at 2 to 6 C
Soluble in DMSO Merck 13, 10074
Store at -20 to 0 C Available Package Sizes
Available Package Sizes 500mg 1g 5g
100mg 1g
C204 - L-Cysteine, Free Base
C375 - Cupric Sulfate (R)-2-Amino-3-mercaptopropanoic Acid
ACS Grade Plant Tissue Culture Tested
Copper(II) Sulfate Pentahydrate Cysteine is readily oxidized to Cystine in neutral to slightly
Plant Tissue Culture Tested alkaline solutions or upon exposure to air in as little as
Powder 1 week at 2 to 6 C. Cystine may precipitate at higher
CAS Number: 7758-99-8 concentrations and a sulfur odor may be present. For short to
Formula: CuSO45H2O long-term storage, Cysteine stocks are more stable in acidic

Biochemicals
Molecular Weight: 249.68 conditions and should be stored at 2 to 6 C.
Soluble in Water Powder
Store at Room Temperature CAS Number: 52-90-4
Merck 13, 2682 Formula: C3H7NO2S
Available Package Sizes Molecular Weight: 121.16
Soluble in Water
250g 500g
Store at Room Temperature
C628 - Cupric Sulfate Pentahydrate, USP Grade Merck 13, 2810
Copper(II) Sulfate Pentahydrate Available Package Sizes
Plant Tissue Culture Tested 100g 500g 1Kg
Powder
CAS Number: 7758-99-8 C248 - L-Cystine, Free Base
Formula: CuSO45H2O [R-(R*,R*)]-3,3-Dithiobis[2-aminopropanoic Acid]
Molecular Weight: 249.68 Plant Tissue Culture Tested
Soluble in Water Cystine is readily soluble in aqueous solutions with pH
Store at Room Temperature above 8 or below 2. It may precipitate out of neutral solutions
Merck 13, 2682 at higher concentrations. Stocks are more stable
Available Package Sizes in the acidic or alkaline conditions and should be stored at 2 to
6 C.
100g 500g 1Kg
Powder
C1830 - Crystal Violet CAS Number: 56-89-3
See Seed Testing Section for Complete Listing Formula: C6H12N2O4S2
Molecular Weight: 240.3
H276 - CTAB Soluble in KOH or HCl; Slightly Soluble in Water
Store at Room Temperature
See Molecular Biology Section for Complete Listing
Merck 13, 2811
H3818 - CTAB Solution (100mg/mL) Available Package Sizes
See Molecular Biology Section for Complete Listing 25g 100g

Biochemicals A-Z Page 27


Product Catalogue & Laboratory Guide
D308 - Dextran Sulfate, Sodium Salt New Product
Powder D2181 - 2,6-Dichloroisonicotinic Acid (INA)
CAS Number: 9011-18-1 INA; 2,6-Dichloropyridine-4-carboxylic Acid
Molecular Weight: 500,000 Avg.
Soluble in Water INA is employed in the biological study of plant pathogen
Store at Room Temperature resistance. It can induce a systemic acquired resistance (SAR)
Merck 13, 2969 response in plants.
Available Package Sizes Plant Tissue Culture Tested
25g 100g 500g Powder
CAS Number: 5398-44-7
D159 - Dicamba Formula: C6H3Cl2NO2
3,6-Dichloro-o-anisic Acid Molecular Weight: 192.00
Plant Tissue Culture Tested Soluble in Methanol; 1% Ethanol
Powder Store at -20C
CAS Number: 1918-00-9 Merck 13, 5206
Formula: C8H6Cl2O3 Available Package Sizes
Molecular Weight: 221.04 1g 5g
Soluble in KOH
Store at 2 to 6 C D295 - 2,4-Dichlorophenoxyacetic Acid
Merck 13, 3065 Solution (1 mg/mL)
Available Package Sizes 2,4-D
Biochemicals

100mg 500mg 1g Sterile Filtered


Plant Tissue Culture Tested
D165 - Dicamba Solution (1 mg/mL) Liquid
3,6-Dichloro-o-anisic Acid CAS Number: 94-75-7
Sterile Filtered Formula: C8H6Cl2O3
Plant Tissue Culture Tested Molecular Weight: 221.04
Liquid Miscible with Water
CAS Number: 1918-00-9 Store at 2 to 6 C
Formula: C8H6Cl2O3
Molecular Weight: 221.04
Miscible with Water
Store at 2 to 6 C Available Package Sizes
Available Package Sizes 100mL 500mL 1L
100mL 25mL
D309 - 2,4-Dichlorophenoxyacetic Acid
D253 - Dichloroisocyanuric Acid, Sodium Salt Solution (10 mg/mL)
NaDCC 2,4-D
Plant Tissue Culture Tested Sterile Filtered
A disinfectant commonly used to surface-sterilize plant tissue. Plant Tissue Culture Tested
Powder Liquid
CAS Number: 2893-78-9 CAS Number: 94-75-7
Formula: C3Cl2N3O3Na Formula: C8H6Cl2O3
Molecular Weight: 219.9 Molecular Weight: 221.04
Soluble in Water Miscible with Water
Store at Room Temperature Store at 2 to 6 C

Available Package Sizes Available Package Sizes


100g 500g 1Kg 100mL 500mL 1L

Page 28 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
D297 - Dikegulac D544 - DNA Neutralizing Solution, pH 7.4
2,3:4,6-Bis-O-(methylethylidene)--L-xylo-2-hexulofuranosonic See Molecular Biology Section for Complete Listing
Acid
Plant Tissue Culture Tested E244 - 2,4-Epibrassinolide
Powder (22R/S, 23R/S, 24R)-2,3,22,23-Tetrahydroxy-B-homo-7-oxa-
CAS Number: 18467-77-1 5-ergostan-6-one
Formula: C12H18O7 Plant Tissue Culture Tested
Molecular Weight: 274.27 Powder
Soluble in DMSO CAS Number: 78821-43-9
Store at 2 to 6 C Formula: C28H48O6
Merck 13, 3222 Molecular Weight: 480.68
Available Package Sizes Slightly soluble in Water, DMSO
Store at -20 to 0 C
1g
Available Package Sizes
D525 - 6-(,-Dimethylallylamino)purine 10mg 100mg
2iP; N6-[2-Isopentyl]adenine
Plant Tissue Culture Tested E410 - Ethylenediaminetetraacetic Acid, Disodium Salt
Powder Na2-EDTA Dihydrate; Disodium Edetate Dihydrate
CAS Number: 2365-40-4 Plant Tissue Culture Tested
Formula: C10H13N5 Powder
Molecular Weight: 203.3 CAS Number: 6381-92-6
Soluble in KOH Formula: C10H14N2O8Na22H2O

Biochemicals
Store at -20 to 0 C Molecular Weight: 372.24
Available Package Sizes Soluble in Water
Store at Room Temperature
100mg 500mg 1g 5g 10g 25g Merck 13, 3543
D217 - 6-(,-Dimethylallylamino)purine Available Package Sizes
Solution (1 mg/mL) 100g 500g 1Kg
2iP; N6-[2-Isopentyl]adenine
Sterile Filtered E349 - Ethylenediamine Bis(2-hydroxyphenylacetic
Plant Tissue Culture Tested Acid), Ferric-Sodium Salt
Liquid FeNa-EDDHA; Ethylenediamine Bis(2-hydroxyphenylacetic
CAS Number: 2365-40-4 Acid), Iron(III)-Sodium Salt
Formula: C10H13N5 Plant Tissue Culture Tested
Molecular Weight: 203.3 Powder
Miscible with Water CAS Number: 16455-61-1
Store at -20 to 0 C Formula: C18H16O6N2NaFe
Available Package Sizes Molecular Weight: 434.8
Soluble in Water
100mL Store at Room Temperature
D259 - Dithiothreitol Available Package Sizes
1,4-Dithiothreitol; DTT; Clelands Reagent 100g 500g 1Kg
Powder
CAS Number: 3483-12-3
Formula: C4H10O2S2
Molecular Weight: 154.2
Soluble in Water
Store at 2 to 6 C
Merck 13, 3412
Available Package Sizes
1g 5g 25g

D548 - DNA Denaturing Solution


See Molecular Biology Section for Complete Listing

Biochemicals A-Z Page 29


Product Catalogue & Laboratory Guide
E676 - Ethylenediaminetetraacetic Acid, Ferric- F3138 - Ferric Ammonium Sulfate
Sodium Salt See Phycology Section for Complete Listing
FeNa-EDTA; Ethylenediaminetetraacetic Acid; Iron(III)-
Sodium Salt; Ferric Sodium Edetate F2876 - Ferric Chloride, Anhydrous
Plant Tissue Culture Tested See Phycology Section for Complete Listing
Powder
F383 - Ferric Chloride, Hexahydrate
CAS Number: 15708-41-5
Formula: C10H12N2O8NaFe Iron(III) Chloride Hexahydrate
Molecular Weight: 367.02 Plant Tissue Culture Tested
Soluble in Water Powder
Store at Room Temperature CAS Number: 10025-77-1
Merck 13, 4062 Formula: FeCl36H2O
Available Package Sizes Molecular Weight: 270.32
Soluble in Water
500g 1Kg 5Kg Store at Room Temperature
Merck 13, 4048
E316 - Ethylenediaminetetraacetic Acid, Free Acid
See Molecular Biology Section for Complete Listing Available Package Sizes
100g 500g
E582 - EDTA Solution 0.5 M, pH 8.0
See Molecular Biology Section for Complete Listing F352 - Ferric Citrate
Iron(III) Citrate
E2799 - Evans Blue Plant Tissue Culture Tested
Biochemicals

Direct Blue 53 Powder


An azo dye commonly used in viability testing; living cells reject CAS Number: 3552-50-7
the dye, while dead cells stain blue. Formula: C6H5O7Fex
Powder Molecular Weight: 244.95
CAS Number: 314-13-6 Soluble in Boiling Water
Formula: C34H24N6Na4O14S4 Store at Room Temperature
Molecular Weight: 960.81 Available Package Sizes
Soluble in Water
250g
Store at Room Temperature
Available Package Sizes F318 - Ferrous Sulfate/Chelate Solution (100x)
10g 50g 100g Iron(II) Sulfate/EDTA Chelated Solution
Sterile Filtered
F3178 - Fast Green (FCF) Stain Plant Tissue Culture Tested
See Seed Testing Section for Complete Listing Use 10.0mL per liter for an equivalent Iron molar
concentration to MS Basal Salts.
Micronutrient Sources Chart Liquid
See "Figure 10. List of Micronutrient Sources & PhytoTech CAS Number: 7782-63-0/ 6381-92-6
Product Numbers" on page 237 in the Technical Section. Formula: FeSO4 7H2O/ C10H14N2O8Na22H2O
Molecular Weight: 278.02/ 372.24
F374 - Ferric Ammonium Citrate Miscible with Water
Ammonium Iron(III) Citrate Store at 2 to 6 C
Plant Tissue Culture Tested Available Package Sizes
Powder 100mL 500mL 1L
CAS Number: 1185-57-5
Formula: C6H8O7Fex(NH3)x
Molecular Weight: Varies
Soluble in Water
Store at Room Temperature
Merck 13, 518
Available Package Sizes
100g 500g

Page 30 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
F388 - Ferric Sulfate Hydrate New Product
Iron(III) Sulfate Hydrate P6622 - flg22 Peptide
Plant Tissue Culture Tested flg22 Peptide is a peptide with its sequence derived from
Powder the flagellin N-terminus of Pseudomonas aeruginosa
CAS Number: 10028-22-5 and is known to elicit specific innate immune response
Formula: Fe2(SO4)3xH2O in plants as well as animals. Amino acid sequence:
Molecular Weight: 399.89+x(18.01) QRLSTGSRINSAKDDAAGLQIA
Soluble in Water Powder
Store at Room Temperature Formula: C93H162N32O34
Merck 13, 4065 Molecular Weight: 2272.5 Avg.
Available Package Sizes Soluble in Water/DMF
250g Store at -20C
Available Package Sizes
F263 - Ferrous Sulfate Heptahydrate
1mg 5mg
Iron(II) Sulfate Heptahydrate
Plant Tissue Culture Tested F357 - Fluridone
Powder 1-Methyl-3-phenyl-5-[3-(trifluoromethyl)phenyl]-4(1H)-
CAS Number: 7782-63-0 pyridinone
Formula: FeSO47H2O Plant Tissue Culture Tested
Molecular Weight: 278.02 Powder
Soluble in Water CAS Number: 59756-60-4
Store at Room Temperature

Biochemicals
Formula: C19H14F3NO
Merck 13, 4091 Molecular Weight: 329.32
Available Package Sizes Soluble in DMSO
100g 500g 1Kg Store at 2 to 6 C
Available Package Sizes
F629 - Ferrous Sulfate Heptahydrate 100mg
Iron(II) Sulfate Heptahydrate
USP Grade F376 - Flurprimidol
Plant Tissue Culture Tested -(1-Methylethyl)--[4-(trifluoromethoxy)phenyl]-5-
Powder pyrimidinemethanol
CAS Number: 7782-63-0 Plant Tissue Culture Tested
Formula: FeSO47H2O Powder
Molecular Weight: 278.02 CAS Number: 56425-91-3
Soluble in Water Formula: C15H15F3N2O2
Store at Room Temperature Molecular Weight: 312.29
Merck 13, 4091 Soluble in DMSO
Available Package Sizes Store at 2 to 6 C
100g 500g Merck 13, 4230
Available Package Sizes
Stains & Dyes Usage Table 10mg 100mg
See "Figure 35. Stains & Dyes Usage Table" on page 264 in
the Technical Section.

Biochemicals A-Z Page 31


Product Catalogue & Laboratory Guide
F430 - Folic Acid G434 - Gellan Gum Powder
N-[4-[[(2-Amino-1,4-dihydro-4-oxo-6-pteridinyl)methyl] CultureGel Type I Biotech Grade
amino]benzoyl]-L-glutamic Acid; Vitamin M Transparency: minimum 80%
Plant Tissue Culture Tested Gel Strength: minimum 800 g/cm2
Powder Plant Tissue Culture Tested
CAS Number: 59-30-3 See also G3251, GelzanTM
Formula: C19H19N7O6 Powder
Molecular Weight: 441.45 CAS Number: 71010-52-1
Soluble in KOH; Slightly Soluble in Water Soluble in Boiling Water
Store at Room Temperature Store at Room Temperature
Merck 13, 4247 Merck 13, 4394
Available Package Sizes Available Package Sizes
5g 25g 2g 100g 500g 1Kg 5Kg
F563 - D-(-)-Fructose New Product
USP/FCC Grade G3500 - Gelling Agent Sample Pack
Fruit Sugar, D-Levulose
Plant Tissue Culture Tested Gelling Agent Sample Pack comes with:
A111 - Tissue Culture Grade Agar: 9g
Powder A175 - Purified Agar: 8g
CAS Number: 57-48-7 G3251 - GelzanTM: 2g
Formula: C6H12O6 A133 - AgargellanTM: 5g
Molecular Weight: 180.16
Biochemicals

C2000 - High Clarity Carrageenan: 10g


Soluble in Water
Store at Room Temperature Contains enough of each gelling agent to make 1L of each
Merck 13, 4295 using the suggested concentration range.
Powder
Available Package Sizes
Soluble in Water
500g 1Kg 5Kg Store at Room Temperature
F356 - Fumaric Acid, Free Acid Available Package Sizes
(E)-2-Butenedioic Acid 1 Each
Plant Tissue Culture Tested
G3251 - Gelzan
Powder
CAS Number: 110-17-8 A Gellan Gum Powder derived from bacterial fermentation by
Formula: C4H4O4 Sphingomonas elodea.
Molecular Weight: 116.07 Transparency: Minimum 80%
Soluble in Water Gel Strength: 400 to 700 g/cm2
Store at Room Temperature Plant Tissue Culture Tested
Merck 13, 4308 Gelzan is a trademark of CP Kelco.
Powder
Available Package Sizes
CAS Number: 71010-52-1
100g 500g Soluble in Boiling Water
Store at Room Temperature
G810 - G418 Sulfate
See Molecular Biology Section for Complete Listing Available Package Sizes
2g 100g 500g 1Kg 5Kg
Gelling Agents Usage Chart
See "Figure 19. Usage Guide for Gelling Agents" on page 246
in the Technical Section.

Page 32 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
G500 - Gibberellic Acid G386 - D-(+)-Glucose Anhydrous
GA3; Gibberellin A3 Dextrose; Corn Sugar
Plant Tissue Culture Tested USP Grade
Powder Plant Tissue Culture Tested
CAS Number: 77-06-5 Powder
Formula: C19H22O6 CAS Number: 50-99-7
Molecular Weight: 346.41 Formula: C6H12O6
Soluble in KOH and Ethanol Molecular Weight: 180.16
Store at 2 to 6 C Soluble in Water
Merck 13, 4430 Store at Room Temperature
Merck 13, 4472
Available Package Sizes
1g 5g Available Package Sizes
500g 1Kg 5Kg 25Kg
G198 - Gibberellic Acid Solution (1 mg/mL)
GA3; Gibberellin A3 G360 - D-(+)-Glucose Monohydrate
Plant Tissue Culture Tested Dextrose Monohydrate; Corn Sugar
Liquid Plant Tissue Culture Tested
CAS Number: 77-06-5 Powder
Formula: C19H22O6 CAS Number: 77938-63-7
Molecular Weight: 346.41 Formula: C6H12O6H2O
Miscible with Water Molecular Weight: 198.2
Store at 2 to 6 C Soluble in Water

Biochemicals
Store at Room Temperature
Available Package Sizes
100mL Available Package Sizes
1Kg 5Kg 25Kg
G362 - Gibberellic Acid Solution (13 mg/mL)
(13,000 ppm) G399 - L-Glutamic Acid
GA3; Gibberellin A3 (S)-2-Aminopentanedioic Acid
Sterile Filtered Plant Tissue Culture Tested
Plant Tissue Culture Tested Powder
Liquid CAS Number: 56-86-0
CAS Number: 77-06-5 Formula: C5H9NO4
Formula: C19H22O6 Molecular Weight: 147.13
Molecular Weight: 346.41 Soluble in Water
Miscible with Water Store at Room Temperature
Store at 2 to 6 C Merck 13, 4482
Merck 13, 4430 Available Package Sizes
Available Package Sizes 100g 1Kg
15mL 100mL
G229 - L-Glutamine
G358 - Gibberellins A4 + A7 (S)-2,5-Diamino-5-oxopentanoic Acid
Plant Tissue Culture Tested Plant Tissue Culture Tested
Powder Powder
Soluble in KOH CAS Number: 56-85-9
Store at 2 to 6 C Formula: C5H10N2O3
Merck 13, 4431 Molecular Weight: 146.14
Soluble in Water
Available Package Sizes Store at Room Temperature
1g Merck 13, 4484

Carbohydrate Selection Guide Available Package Sizes


See "Figure 12. Choosing a Carbohydrate" on page 238 in the 100g 500g 1Kg 5Kg
Technical Section.

Biochemicals A-Z Page 33


Product Catalogue & Laboratory Guide
G3399 - Glutathione (Reduced), Free Acid G3574 - Guaiacol
-L-Glutamyl-L-cysteinyl-glycine See Seed Testing Section for Complete Listing
Plant Tissue Culture Tested
Powder
G3300 - Guanidine Thiocyanate
CAS Number: 70-80-8 See Molecular Biology Section for Complete Listing
Formula: C10H17N3O6S
Molecular Weight: 307.33 H326 - HEPES, Free Acid
Soluble in Water See Molecular Biology Section for Complete Listing
Store at 2 to 6 C
New Product
Available Package Sizes
H4000 - (Z)3Hexenol
1g 10g
cis-3-Hexen-1-ol; leaf alcohol
G381 - Glycerol
Glycerin; 1,2,3-Propanetriol Plant Defense chemical that when airborne can increase levels
Plant Tissue Culture Tested of HexVic ((Z)-3-hexynl-vicianoside) in un-assaulted tomato
leaves, a chemical that reduces growth of larvae.
Liquid Plant Tissue Culture Tested
CAS Number: 56-81-5
Formula: C3H8O3 Liquid
Molecular Weight: 92.09 CAS Number: 928-96-1
Miscible with Water Formula: C6H12O
Store at Room Temperature Molecular Weight: 100.16
Merck 13, 4497 Miscible with Water
Biochemicals

Store at Room Temperature


Available Package Sizes Merck 13, 4719
100mL 500mL 1L
Available Package Sizes
Amino Acids Used in Plant Tissue Culture 100mL 250mL
See "Figure 14. List of Amino Acids by Type & PhytoTech
Product Numbers" on page 240 in the Technical Section.
H397 - Hygromycin B
See Molecular Biology Section for Complete Listing
G503 - Glycine
Aminoacetic Acid
H370 - Hygromycin B Solution, 50 mg/mL in
Plant Tissue Culture Tested PBS Buffer
Powder See Molecular Biology Section for Complete Listing
CAS Number: 56-40-6
Formula: C2H5NO2
H385 - Hygromycin B Solution, 100 mg/mL in
Molecular Weight: 75.08 Distilled Water
Soluble in Water See Molecular Biology Section for Complete Listing
Store at Room Temperature
Merck 13, 4504 I4068 - Indigo Carmine
Available Package Sizes See Seed Testing Section for Complete Listing
100g 500g 1Kg I885 - Indole-3-Acetic Acid
G345 - Glyphosate IAA; Heteroauxin
Plant Tissue Culture Tested
N-(Phosphonomethyl)glycine
Plant Tissue Culture Tested Powder
CAS Number: 87-51-4
Powder
Formula: C10H9NO2
CAS Number: 1071-83-6
Molecular Weight: 175.19
Formula: C3H8NO5P
Soluble in KOH
Molecular Weight: 169.07
Store at -20 to 0 C
Soluble in Water
Merck 13, 4986
Store at 2 to 6 C
Merck 13, 4525 Available Package Sizes
Available Package Sizes 5g 25g 100g
1g 5g

Page 34 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
I364 - Indole-3-Acetic Acid Solution (1 mg/mL) I460 - Indole-3-Butyric Acid Solution (1 mg/mL)
IAA; Heteroauxin IBA; 4-[3-Indolyl]butyric Acid
Sterile Filtered Sterile Filtered
Plant Tissue Culture Tested Plant Tissue Culture Tested
Liquid Liquid
CAS Number: 87-51-4 CAS Number: 133-32-4
Formula: C10H9NO2 Formula: C12H13NO2
Molecular Weight: 175.19 Molecular Weight: 203.24
Miscible with Water Miscible with Water
Store at -20 to 0 C Store at -20 to 0 C
Light Sensitive Light Sensitive
Merck 13, 4986 Merck 13, 4987
Available Package Sizes Available Package Sizes
100mL 100mL

I538 - Indole-3-Butyric Acid I409 - Indole-3-Propionic Acid


IBA; 4-[3-Indolyl]butyric Acid IPA
Plant Tissue Culture Tested Plant Tissue Culture Tested
Powder Powder
CAS Number: 133-32-4 CAS Number: 830-96-6
Formula: C12H13NO2 Formula: C11H11NO2
Molecular Weight: 203.24 Molecular Weight: 189.2

Biochemicals
Soluble in KOH Soluble in KOH
Store at 2 to 6 C Store at 2 to 6 C
Merck 13, 4987 Available Package Sizes
Available Package Sizes 5g
5g 25g 100g
I703 - myo-Inositol
Plant Growth Regulators Usage and Storage i-Inositol; meso-Inositol;
Chart 1,2,3,5/4,6-Hexahydroxycyclohexane
See "Figure 18. Plant Growth Regulators Usage and Storage Plant Tissue Culture Tested
Chart" on page 244 in the Technical Section. Powder
CAS Number: 87-89-8
I560 - Indole-3-Butyric Acid, Potassium Salt Formula: C6H12O6
K-IBA; 4-[3-Indolyl]butyric Acid, Potassium Salt Molecular Weight: 180.16
Plant Tissue Culture Tested Soluble in Water
Powder Store at Room Temperature
CAS Number: 60096-23-3 Merck 13, 5001
Formula: C12H12NO2K Available Package Sizes
Molecular Weight: 241.3 100g 500g 1Kg 5Kg
Soluble in Water
Store at 2 to 6 C I331 - Iron Chelate
Available Package Sizes Sequestrene 330;
5g 25g 100g Sodium ferric diethylenetriaminepentaacetate; NaFe - DTPA
Plant Tissue Culture Tested
Powder
Soluble in Water
Store at Room Temperature
Available Package Sizes
100g 500g

I373 - Isopropyl--D-thiogalactopyranoside
See Molecular Biology Section for Complete Listing

Biochemicals A-Z Page 35


Product Catalogue & Laboratory Guide
New Product K483 - Kinetin Solution (1 mg/mL)
J389 - Methyl Jasmonate (MeJA) 6-Furfurylaminopurine
Sterile Filtered
MeJA; 2-Pentenylcyclopentanone-3-acetic acid; Methyl 3-oxo-
Plant Tissue Culture Tested
2-(pent-2-enyl)cyclopentaneacetate
Liquid
Methyl Jasmonate is a key signaling hormone associated CAS Number: 525-79-1
with necrotropic/herbivore stress which affects plant defense Formula: C10H9N5O
responses as well as growth and development. Molecular Weight: 215.2
Plant Tissue Culture Tested Miscible with Water
Store at -20 to 0 C
Liquid
Merck 13, 5329
CAS Number: 39924-52-2
Formula: C13H20O3 Available Package Sizes
Molecular Weight: 224.3 100mL 500mL
Miscible with EtOH
Store from 2 to 6 C L451 - Lanolin
Merck 13, 5279 Wool Fat; Lanolin Wax
Available Package Sizes Waxy Solid
5mL 25mL CAS Number: 8006-54-0
Soluble in Hot Alcohol, Chloroform and Ether
K378 - Kanamycin Monosulfate Store at Room Temperature
See Molecular Biology Section for Complete Listing Merck 13, 5001
Available Package Sizes
Biochemicals

K586 - Kanamycin Monosulfate Solution (50 mg/mL)


454g
See Molecular Biology Section for Complete Listing
L574 - L-Leucine
K4751 - Kanamycin Monosulfate
(S)-2-Amino-4-methylpentanoic Acid
Solution (100 mg/mL)
Plant Tissue Culture Tested
See Molecular Biology Section for Complete Listing
Powder
K559 - Kasugamycin Hydrochloride Monohydrate CAS Number: 61-90-5
Formula: C6H13NO2
See Molecular Biology Section for Complete Listing Molecular Weight: 131.2
Soluble in Water
K424 - Keiki Paste
Store at Room Temperature
Contains Lanolin, trans-Cinnamic Acid, myo-Inositol, Merck 13, 5470
6-Benzylaminopurine, Thiamine HCl, and Adenine
Hemisulfate Available Package Sizes
Paste 100g 500g
Store at Room Temperature
L594 - L-Lysine Monohydrochloride
Available Package Sizes (S)-2,6-Diaminohexanoic Acid Monohydrochloride
50mL Plant Tissue Culture Tested
Powder
K750 - Kinetin
CAS Number: 657-27-2
6-Furfurylaminopurine Formula: C6H14O2N2HCl
Plant Tissue Culture Tested Molecular Weight: 182.65
Powder Soluble in Water
CAS Number: 525-79-1 Store at Room Temperature
Formula: C10H9N5O Merck 13, 5656
Molecular Weight: 215.2
Available Package Sizes
Soluble in KOH
Store at -20 to 0 C 100g 500g 1Kg
Merck 13, 5329
Available Package Sizes
1g 5g 25g

Page 36 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
M521 - Magnesium Chloride Hexahydrate M635 - Magnesium Sulfate Anhydrous, USP
Plant Tissue Culture Tested Grade
Powder USP Grade
CAS Number: 7791-18-6 Plant Tissue Culture Tested
Formula: MgCl26H2O Powder
Molecular Weight: 203.3 CAS Number: 7487-88-9
Soluble in Water Formula: MgSO4
Store at Room Temperature Molecular Weight: 120.37
Merck 13, 5684 Soluble in Water
Available Package Sizes Store at Room Temperature
Merck 13, 5715
100g 500g 1Kg
Available Package Sizes
M542 - Magnesium Nitrate Hexahydrate 1Kg
Nitromagnesite
Plant Tissue Culture Tested M573 - Malachite Green Oxalate
Powder Basic Green; Aniline Green
CAS Number: 13446-18-9 Powder
Formula: Mg(NO3)26H2O CAS Number: 2437-29-8
Molecular Weight: 256.41 C23H25N2C2HO40.5C2H2O4
Soluble in Water Molecular Weight: 463.5
Store at Room Temperature Soluble in Water and Ethanol
Merck 13, 5697 Store at Room Temperature

Biochemicals
Available Package Sizes Merck 13, 5722
500g 1Kg 5Kg

M200 - Magnesium Sulfate Heptahydrate Available Package Sizes


Epsom Salts 25g 100g
Plant Tissue Culture Tested
Powder M471 - L-Maleic Acid
CAS Number: 10034-99-8 cis-Butenedioic Acid; cis-1,2-Ethylenedicarboxylic Acid
Formula: MgSO47H2O Plant Tissue Culture Tested
Molecular Weight: 246.48 Powder
Soluble in Water CAS Number: 110-16-7
Store at Room Temperature Formula: C4H4O4
Merck 13, 5715 Molecular Weight: 116.07
Available Package Sizes Soluble in Water
Store at Room Temperature
500g 1Kg 5Kg 10Kg
Merck 13, 5726
M150 - Magnesium Sulfate Anhydrous Available Package Sizes
Plant Tissue Culture Tested 100g 500g
Powder
CAS Number: 7487-88-9 M494 - Maleic Acid Hydrazide
Formula: MgSO4 Maleic Hydrazide; 1,2-Dihydro-3,6-pyridazinedione
Molecular Weight: 120.37 Plant Tissue Culture Tested
Soluble in Water Powder
Store at Room Temperature CAS Number: 123-33-1
Merck 13, 5715 Formula: C4H4N2O2
Available Package Sizes Molecular Weight: 112.10
Soluble in Hot Water and DMSO
500g 1Kg
Store at Room Temperature
Merck 13, 5728
Available Package Sizes
25g 100g

Biochemicals A-Z Page 37


Product Catalogue & Laboratory Guide
M5536 - DL-Malic Acid M250 - Manganese Sulfate Monohydrate
DL-Hydroxybutanedioic acid Plant Tissue Culture Tested
Plant Tissue Culture Tested Powder
Powder CAS Number: 10034-96-5
CAS Number: 6915-15-7 Formula: MnSO4H2O
Formula: C4H6O5 Molecular Weight: 169.02
Molecular Weight: 134.09 Soluble in Water
Soluble in Water Store at Room Temperature
Store at Room Temperature Merck 13, 5763
Merck 13, 5730 Available Package Sizes
Available Package Sizes 100g 500g
500g 1Kg 5Kg
M637 - Manganese Sulfate Monohydrate, USP
M474 - Malt Extract Grade
Co-extract of Corn and Malted Barley USP Grade
Plant Tissue Culture Tested Plant Tissue Culture Tested
Powder Powder
CAS Number: 8002-48-0 CAS Number: 10034-96-5
Soluble in Water Formula: MnSO4H2O
Store at Room Temperature Molecular Weight: 169.02
Available Package Sizes Soluble in Water
Store at Room Temperature
Biochemicals

500g 1Kg
Merck 13, 5763
M588 - D-Maltose Monohydrate Available Package Sizes
4-O--D-Glucopyranosyl-D-glucose 100g 500g
Plant Tissue Culture Tested
Powder M562 - D-Mannitol
CAS Number: 6363-53-7 USP Grade
Formula: C12H22O11H2O Plant Tissue Culture Tested
Molecular Weight: 360.32 Powder
Soluble in Water CAS Number: 69-65-8
Store at Room Temperature Formula: C6H14O6
Merck 13, 5736 Molecular Weight: 182.2
Available Package Sizes Soluble in Water
Store at Room Temperature
100g 500g 1Kg 5Kg 10Kg 25Kg
Merck 13, 5769
M455 - Manganese Chloride Tetrahydrate Available Package Sizes
Plant Tissue Culture Tested 500g 1Kg 5Kg
Powder
CAS Number: 13446-34-9 M486 - D-(+)-Mannose
Formula: MnCl24H2O Plant Tissue Culture Tested
Molecular Weight: 197.91 Powder
Soluble in Water CAS Number: 3458-28-4
Store at Room Temperature Formula: C6H12O6
Merck 13, 5751 Molecular Weight: 180.16
Available Package Sizes Soluble in Water
Store at Room Temperature
100g
Merck 13, 5772
Available Package Sizes
25g 250g

Page 38 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
M567 - Meat Extract F323 - Media Dye, Red
Plant Tissue Culture Tested Erythrosine; FD&C Red #3
Powder Plant Tissue Culture Tested
Soluble in Water Recommended 100 mg to 250 mg per liter.
Store at Room Temperature For our media coloration sampler containing four different
media dyes, see product M5856.
Available Package Sizes
Powder
500g 1Kg
CAS Number: 16423-68-0
M5856 - Media Coloration Sampler Formula: C20H6I4Na2O5
Molecular Weight: 879.86
Media Coloration Sampler comes with four 1 gram package
Soluble in Water
sizes of red (Product Number F323), green (Product Number
Store at Room Temperature
F320), blue (Product Number F322), and yellow (Product
Merck 13, 3727
Number F321) media dyes.
Plant Tissue Culture Tested Available Package Sizes
Recommended 0.01 to 0.1g per liter. 1g 10g 100g
Powder
F321 - Media Dye, Yellow
Soluble in Water
Store at Room Temperature Tartrazine; FD&C Yellow #5
Plant Tissue Culture Tested
Available Package Sizes
Recommended 100 mg to 250 mg per liter.
4x1g Kit For our media coloration sampler containing four different

Biochemicals
media dyes, see product M5856.
F322 - Media Dye, Blue
Powder
Brilliant Blue FCF; FD&C Blue #1
CAS Number: 1934-21-0
Plant Tissue Culture Tested
Formula: C16H9N4Na3O9S2
Recommended 100 mg to 250 mg per liter. Molecular Weight: 534.37
For our media coloration sampler containing four different Soluble in Water
media dyes, see product M5856. Store at Room Temperature
Powder Merck 13, 9160
CAS Number: 3844-45-9 Available Package Sizes
Formula: C37H34N2Na2O9S3
Molecular Weight: 792.86 1g 10g 100g
Soluble in Water
M5781 - Methylene Blue
Store at Room Temperature
Merck 13, 1359 See Seed Testing Section for Complete Listing

Available Package Sizes M539 - L-Methionine


1g 10g 100g (S)-2-Amino-4-(methylthio)butanoic Acid
Plant Tissue Culture Tested
F320 - Media Dye, Green
Powder
Pyla-Cert Green MX-415 CAS Number: 63-68-3
A mixture of FD&C Green #3 and FD&C Yellow #5 Formula: C5H11NO2S
Plant Tissue Culture Tested Molecular Weight: 149.21
Recommended 100 mg to 250 mg per liter. Soluble in Water
For our media coloration sampler containing four different Store at Room Temperature
media dyes, see product M5856. Merck 13, 6004
Powder Available Package Sizes
Soluble in Water
25g 100g
Store at Room Temperature
Available Package Sizes
1g 10g 100g

Biochemicals A-Z Page 39


Product Catalogue & Laboratory Guide
M488 - Molybdenum Trioxide N605 - -Naphthaleneacetic Acid Solution (1 mg/mL)
Molybdenum(VI) Oxide; Molybdic Anhydride NAA; 1-Naphthaleneacetic Acid
Plant Tissue Culture Tested Sterile Filtered
Powder Plant Tissue Culture Tested
CAS Number: 1313-27-5 Liquid
Formula: MoO3 CAS Number: 86-87-3
Molecular Weight: 143.94 Formula: C12H10O2
Soluble in NaOH or KOH; Slightly soluble Molecular Weight: 186.2
in Water Miscible with Water
Store at Room Temperature Store at 2 to 6 C
Merck 13, 6261 Merck 13, 6397
Available Package Sizes Available Package Sizes
100g 500g 100mL 500mL 1L

M572 - MOPS-EDTA-Sodium Acetate Buffer N610 - -Naphthaleneacetic Acid, Potassium Salt


Solution (10x) K-NAA; 1-Naphthaleneacetic Acid, Potassium Salt
See Molecular Biology Section for Complete Listing Plant Tissue Culture Tested
Powder
M631 - MOPS, Free Acid CAS Number: 15165-79-4
See Molecular Biology Section for Complete Listing Formula: C12H9KO2
Molecular Weight: 224.3
M565 - MTT Soluble in Water
Biochemicals

See Molecular Biology Section for Complete Listing Store at Room Temperature
Available Package Sizes
M569 - MUG
5g 25g 100g
See Molecular Biology Section for Complete Listing
N6179 - 1-Naphthol
Plant Growth Regulators Usage and Storage
-Naphthol
Chart
Has been used for staining or visualization of glycoglycerolipids
See "Figure 18. Plant Growth Regulators Usage and Storage and in Molischs test for the detection of carbohydrates.
Chart" on page 244 in the Technical Section. Applying 1-Naphthol to barley seeds stimulates seedling growth
significantly.
N600 - -Naphthaleneacetic Acid
Powder
NAA; 1-Naphthaleneacetic Acid CAS Number: 90-15-3
Plant Tissue Culture Tested Formula: C10H8O
Powder Molecular Weight: 144.17
CAS Number: 86-87-3 Soluble in Ethanol
Formula: C12H10O2 Store at Room Temperature
Molecular Weight: 186.2
Soluble in KOH
Available Package Sizes
Store at Room Temperature 10g 100g
Merck 13, 6397
N564 - -Naphthoxyacetic Acid
Available Package Sizes
BNOA; O-(2-Naphthyl)glycolic Acid
25g 100g Plant Tissue Culture Tested
Powder
CAS Number: 120-23-0
Formula: C12H10O3
Molecular Weight: 202.21
Soluble in DMSO
Store at Room Temperature
Merck 13, 6423
Available Package Sizes
25g 100g

Page 40 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
N609 - Niacinamide N617 - Nitric Acid Solution 1.0 N
Nicotinamide; 3-Pyridinecarboxamide; Vitamin B3; Aquafortis
Vitamin PP Plant Tissue Culture Tested
Plant Tissue Culture Tested Liquid
Powder CAS Number: 7697-37-2
CAS Number: 98-92-0 Formula: HNO3
Formula: C6H6N2O Molecular Weight: 63.01
Molecular Weight: 122.12 Miscible with Water
Soluble in Water Store at Room Temperature
Store at Room Temperature Merck 13, 6608
Merck 13, 6550
Available Package Sizes
25g 100g Available Package Sizes
1L 4L
N478 - Nickel (II) Chloride Hexahydrate
Plant Tissue Culture Tested N602 - Nitroblue Tetrazolium
Powder NBT; 2,2-Di--nitrophenyl-5,5-diphenyl-3,3-(3,3-
CAS Number: 7791-20-0 dimethoxy-4,4-diphenylene)-ditetrazolium Chloride
Formula: NiCl26H2O Powder
Molecular Weight: 237.7 CAS Number: 298-83-9
Soluble in Water Formula: C40H30Cl2N10O6
Store at Room Temperature Molecular Weight: 817.6

Biochemicals
Merck 13, 6529 Soluble in DMSO
Available Package Sizes Store at 2 to 6 C
5g 100g Available Package Sizes
100mg 1g
N458 - Nickel (II) Sulfate Hexahydrate
Plant Tissue Culture Tested N604 - 2-Nitrophenyl--D-galactopyranoside
Powder See Molecular Biology Section for Complete Listing
CAS Number: 10101-97-0
Formula: NiSO46H2O N618 - 4-Nitrophenyl--D-glucuronide
Molecular Weight: 262.84 See Molecular Biology Section for Complete Listing
Soluble in Water
Store at Room Temperature O626 - L-Ornithine Hydrochloride
Merck 13, 6541 2,5-Diaminopentanoic Acid Hydrochloride
Available Package Sizes Plant Tissue Culture Tested
5g 100g 500g 1Kg Powder
CAS Number: 3184-13-2
N765 - Nicotinic Acid Formula: C5H12N2O2HCl
Molecular Weight: 168.65
Niacin; Pyridine-3-carboxylic Acid
Soluble in Water
Plant Tissue Culture Tested
Store at Room Temperature
Powder
CAS Number: 59-67-6 Available Package Sizes
Formula: C6H5NO2 25g 100g
Molecular Weight: 123.12
Soluble in Water Stains & Dyes Usage Table
Store at Room Temperature See "Figure 35. Stains & Dyes Usage Table" on page 264 in
Merck 13, 6552 the Technical Section.
Available Package Sizes
100g 500g

Biochemicals A-Z Page 41


Product Catalogue & Laboratory Guide
O6350 - Orange G P775 - Peptone, Type 1, Peptic Digest of Meat
Acid Orange 10; 7-Hydroxy-8-phenylazo-1,3-naphthalene Plant Tissue Culture Tested
disulphonic acid disodium salt; Wool Orange 2G Powder
Orange G is a plant tissue culture stain, and one of the three Soluble in Water
components in Flemmings triple stain. It is an azo dye used Store at Room Temperature
primarily as a histological stain.
Available Package Sizes
Powder
CAS Number: 1936-15-8 100g 500g 1Kg
Formula: C16H10N2Na2O7S2
Molecular Weight: 452.37
P780 - Peptone, Glysate
Soluble in Water Enzymatic Digest of Gelatin
Store at Room Temperature Plant Tissue Culture Tested
Available Package Sizes Powder
Soluble in Water
25g 100g Store at Room Temperature
O630 - Oryzalin Available Package Sizes
4-(Dipropylamino)-3,5-dinitrobenzenesulfonamide 500g 1Kg
Plant Tissue Culture Tested
Powder
P721 - Peptone, From Soymeal
CAS Number: 19044-88-3 Papainic; Enzymatic Digest of Soybean
Formula: C12H18N4O6S Plant Tissue Culture Tested
Molecular Weight: 346.36 Powder
Biochemicals

Soluble in DMSO Soluble in Water


Store at Room Temperature Store at Room Temperature
Merck 13, 6953
Available Package Sizes
Available Package Sizes 500g 1Kg
500mg 1g
P778 - L-Phenylalanine
P687 - Paclobutrazol (S)-2-amino-3-phenylpropanoic acid
1-tert-butyl-2-(p-chlorobenzyl)-2-(1,2,4-triazol-1-yl)ethanol Plant Tissue Culture Tested
Plant Tissue Culture Tested Powder
Powder CAS Number: 63-91-2
CAS Number: 76738-62-0 Formula: C9H11NO2
Formula: C15H20ClN3O Molecular Weight: 165.19
Molecular Weight: 293.80 Soluble in Water
Soluble in DMSO Store at Room Temperature
Store at Room Temperature Merck 13, 7355
Merck 13, 7053
Available Package Sizes
Available Package Sizes 25g 100g 500g
25g 100g

P6737 - Pentachloronitrobenzene (PCNB)


See Seed Testing Section for Complete Listing

P777 - Penicillin G
See Seed Testing Section for Complete Listing

P6767 - Penicillin G Solution (10 mg/mL)


See Seed Testing Section for Complete Listing

Page 42 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
New Product P679 - DL-Phosphinothricin, Monoammonium Salt
A1000 - Phyto-Organic Agar See Molecular Biology Section for Complete Listing
Phyto-Organic Products are sourced from the Organic Material G523 - DL-Phosphinothricin Solution (1 mg/mL)
Review Institutes (OMRI) list of organic chemicals and are
Certified Organic. For use in laboratory research only. See Molecular Biology Section for Complete Listing

Agar-Agar; Gum Agar. Derivied from Gelidium species base.


P694 - Phloroglucinol
Gel Strength: minimum 500 g/cm2 1,3,5-Trihydroxybenzene
Plant Tissue Culture Tested Plant Tissue Culture Tested
Powder Powder
CAS Number: 9002-18-0 CAS Number: 108-73-6
Soluble in Boiling Water Formula: C6H6O3
Store at Room Temperature Molecular Weight: 126.12
Merck 13, 184 Soluble in Water
Store at Room Temperature
Available Package Sizes Merck 13, 7413
9g 100g 500g 1Kg
Available Package Sizes
New Product 25g 100g
C1780 - Phyto-Organic Freeze-Dried Coconut P717 - Picloram
Powder 4-Amino-3,5,6-trichloropicolinic Acid
Phyto-Organic Products are sourced from the Organic Material Plant Tissue Culture Tested

Biochemicals
Review Institutes (OMRI) list of organic chemicals and are
Powder
Certified Organic. For use in laboratory research only.
CAS Number: 1918-02-1
Formula: C6H3Cl3N2O2
Plant Tissue Culture Tested
Molecular Weight: 241.48
Powder Soluble in DMSO
Suspension in Water Store at Room Temperature
Store at Room Temperature Merck 13, 7482
Available Package Sizes Available Package Sizes
100g 500g 1Kg 100mg 1g 5g 10g
New Product P862 - Pineapple Powder
S7761 - Phyto-Organic Sucrose A mixture of natural pineapple puree and maltodextrin.
Phyto-Organic Products are sourced from the Organic Material Plant Tissue Culture Tested
Review Institutes (OMRI) list of organic chemicals and are Powder
Certified Organic. For use in laboratory research only. Suspension in Water
Store at Room Temperature
-D-Fructofuranosyl--D-glucopyranoside; D(+)saccharose;
Available Package Sizes
Cane Sugar
Plant Tissue Culture Tested 500g 1Kg
Crystalline P770 - Pluronic F-68
CAS Number: 57-50-1
Poloxamer 188
Formula: C12H22O11
Plant Tissue Culture Tested
Molecular Weight: 342.34
Pluronic is a registered trademark of BASF Corporation.
Soluble in Water
Store at Room Temperature Powder
Merck 13, 8966 CAS Number: 9003-11-6
Formula: (C3H6OC2H4O)x
Available Package Sizes Molecular Weight: 8400 Avg.
30g 100g 500g 1Kg 5kg Soluble in Water
Store at Room Temperature
P711 - PIPES Merck 13, 7645
See Molecular Biology Section for Complete Listing
Available Package Sizes
100g

Biochemicals A-Z Page 43


Product Catalogue & Laboratory Guide
P714 - Polyethylene Glycol, PEG-3350 P720 - Polyoxyethylenesorbitan Monolaurate
Carbowax Tween 20; Polysorbate 20
Plant Tissue Culture Tested Plant Tissue Culture Tested
Powder Tween is a registered trademark of ICI America, Inc.
CAS Number: 25322-68-3 Liquid
Formula: H(C2H4O)nOH CAS Number: 9005-64-5
Molecular Weight: 3000-3700 Formula: C58H114O26
Soluble in Water Molecular Weight: 1227.5
Store at Room Temperature Miscible with Water
Merck 13, 7651 Store at Room Temperature
Merck 13, 7664
Available Package Sizes
1Kg 5Kg Available Package Sizes
100mL 500mL 1L
P725 - Polyethylene Glycol , PEG-1500
Carbowax P738 - Polyoxyethylenesorbitan Monooleate
Plant Tissue Culture Tested Tween 80; Polysorbate 80
Waxy Solid Plant Tissue Culture Tested
CAS Number: 25322-68-3 Tween is a registered trademark of ICI America, Inc.
Formula: H(C2H4O)nOH Liquid
Molecular Weight: 1300-1600 CAS Number: 9005-65-6
Soluble in Water Formula: C64H124O26
Store at Room Temperature Molecular Weight: 1309.63
Biochemicals

Merck 13, 7651 Miscible with Water


Available Package Sizes Store at Room Temperature
Merck 13, 7664
500g 1Kg 5Kg 10Kg
Available Package Sizes
P741 - Polyethylene Glycol, PEG-8000 100mL 500mL 1L
Carbowax
Plant Tissue Culture Tested P728 - Polyvinylpyrrolidone
Powder PVP-40; Povidone
CAS Number: 25322-68-3 Plant Tissue Culture Tested
Formula: H(C2H4O)nOH Powder
Molecular Weight: 7000-9000 CAS Number: 9003-39-8
Soluble in Water Formula: (C6H9NO)n
Store at Room Temperature Molecular Weight: 40,000 Average
Merck 13, 7651 Soluble in Water
Available Package Sizes Store at Room Temperature
Merck 13, 7783
1 Kg
Available Package Sizes
P6809 - Polymyxin B Sulfate 100g 500g 1Kg
See Seed Testing Section for Complete Listing
P661 - Potassium Acetate
P6719 - Polymyxin B Solution (10 mg/mL) Plant Tissue Culture Tested
See Seed Testing Section for Complete Listing
Powder
CAS Number: 127-08-2
Formula: KC2H3O2
Molecular Weight: 98.14
Soluble in Water
Store at Room Temperature
Merck 13, 7687
Available Package Sizes
100g 500g 1Kg

Page 44 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
P671 - Potassium Acetate Solution 3.0 M, pH 5.0 P672 - Potassium Hydroxide
See Molecular Biology Section for Complete Listing Plant Tissue Culture Tested
Pellets
P6905 - Potassium Bromide CAS Number: 1310-58-3
Commonly used as FTIR matrix. Formula: KOH
Powder Molecular Weight: 56.11
CAS Number: 7758-02-3 Soluble in Water
Formula: KBr Store at Room Temperature
Molecular Weight: 119.0 Merck 13, 7724
Soluble in Water Available Package Sizes
Store at Room Temperature
Merck 13, 7701 500g 1Kg 5Kg

Available Package Sizes P682 - Potassium Hydroxide Solution 1.0 N


100g Plant Tissue Culture Tested
Liquid
P614 - Potassium Carbonate CAS Number: 1310-58-3
Sesquihydrate Formula: KOH
Plant Tissue Culture Tested Molecular Weight: 56.11
Powder Miscible with Water
CAS Number: 6381-79-9 Store at Room Temperature
Formula: K2CO31.5H2O Merck 13, 7724
Molecular Weight: 165.23

Biochemicals
Soluble in Water
Store at Room Temperature
Available Package Sizes
Merck 13, 7702
500mL 1L 4L
Available Package Sizes
100g 500g 1Kg P840 - Potassium Iodide
Plant Tissue Culture Tested
P704 - Potassium Chloride
Powder
Plant Tissue Culture Tested CAS Number: 7681-11-0
Powder Formula: KI
CAS Number: 7447-40-7 Molecular Weight: 166.0
Formula: KCl Soluble in Water
Molecular Weight: 74.55 Store at Room Temperature
Soluble in Water Merck 13, 7727
Store at Room Temperature
Available Package Sizes
Merck 13, 7704
100g 500g
Available Package Sizes
500g 1Kg 5Kg P640 - Potassium Iodide, USP Grade
USP Grade
P729 - Potassium Citrate Monohydrate Plant Tissue Culture Tested
Tripotassium Citrate Monohydrate Powder
Plant Tissue Culture Tested CAS Number: 7681-11-0
Powder Formula: KI
CAS Number: 6100-05-6 Molecular Weight: 166
Formula: C6H5K3O7H2O Soluble in Water
Molecular Weight: 324.41 Store at Room Temperature
Soluble in Water Merck 13, 7727
Store at Room Temperature
Available Package Sizes
Available Package Sizes 100g
500g
Macronutriens Sources Chart
See "Figure 9. List of Macronutrient Sources & PhytoTech
Product Numbers" on page 236 in the Technical Section.

Biochemicals A-Z Page 45


Product Catalogue & Laboratory Guide
P100 - Potassium Nitrate Undefined Organic Supplements Chart
Plant Tissue Culture Tested See "Figure 15. List of Common Undefined Organic
Powder Supplements & PhytoTech Product Numbers" on page 240
CAS Number: 7757-79-1 in the Technical Section.
Formula: KNO3
Molecular Weight: 101.10
P692 - Potato Powder
Soluble in Water Plant Tissue Culture Tested
Store at Room Temperature Powder
Merck 13, 7733 Suspension in Water
Available Package Sizes Store at Room Temperature
500g 1Kg 5Kg 10Kg 15Kg Available Package Sizes
100g 500g 1Kg
P705 - Potassium Phosphate, Dibasic Anhydrous
Dipotassium Phosphate; Dipotassium Hydrogen Phosphate New Product
Plant Tissue Culture Tested P6847 - Prohexadione Calcium
Powder BX-112, 3,5-Dioxo-4-propionyl-cyclohexanecarboxylic acid
CAS Number: 7758-11-4 calcium salt.
Formula: K2HPO4
Molecular Weight: 174.18 Plant growth regulator with anti-gibberellin activity.
Soluble in Water Plant Tissue Culture Tested
Store at Room Temperature
Powder
Merck 13, 7743
Biochemicals

CAS Number: 127277-53-6


Available Package Sizes Formula: C10H10CaO5
100g 500g 1Kg Molecular Weight: 250.26
Soluble in Water
P846 - Potassium Phosphate, Monobasic Anhydrous Store at Room Temperature
Monopotassium Phosphate; Potassium Dihydrogen Phosphate Merck 13, 7868
Plant Tissue Culture Tested Available Package Sizes
Powder 100mg 1g
CAS Number: 7778-77-0
Formula: KH2PO4 P698 - L-Proline
Molecular Weight: 136.09 (S)-2-Pyrrolidinecarboxylic Acid
Soluble in Water Plant Tissue Culture Tested
Store at Room Temperature
Powder
Merck 13, 7744
CAS Number: 147-85-3
Available Package Sizes Formula: C5H9NO2
100g 500g 1Kg 5Kg 10Kg Molecular Weight: 115.13
Soluble in Water
P6780 - Potassium Sodium Tartrate Store at Room Temperature
See Seed Testing Section for Complete Listing Merck 13, 7871
Available Package Sizes
P854 - Potassium Sulfate, Anhydrous
100g 500g 1Kg
Plant Tissue Culture Tested
Powder Amino Acids Used in Plant Tissue Culture
CAS Number: 7778-80-5 See "Figure 14. List of Amino Acids by Type & PhytoTech
Formula: K2SO4 Product Numbers" on page 240 in the Technical Section.
Molecular Weight: 174.26
Soluble in Water
Store at Room Temperature
Merck 13, 7759
Available Package Sizes
500g 1Kg 5Kg

Page 46 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
P6698 - L-Proline 2.5 M Solution P684 - Pyruvic Acid, Potassium Salt
(S)-2-Pyrrolidinecarboxylic Acid -Ketopropionic Acid, Potassium Salt
Sterile Filtered Plant Tissue Culture Tested
Plant Tissue Culture Tested Powder
Liquid CAS Number: 4151-33-1
CAS Number: 147-85-3 Formula: C3H3KO3
Formula: C5H9NO2 Molecular Weight: 126.15
Molecular Weight: 115.13 Soluble in Water
Miscible with Water Store at 2 to 6 C
Store at 2 to 6 C Available Package Sizes
Merck 13, 7871
25g 100g
Available Package Sizes
25mL 100mL R7228 - Rhodamine B
Basic Violet 10, Brilliant Pink B, Rhodamine O,
P733 - Putrescine Dihydrochloride Tetraethylrhodamine
1,4-Butanediamine Dihydrochloride; Tetramethylenediamine Rhodamine B is a protein-specific fluorescent stain commonly
Dihydrochloride used in fluorescence microscopy; it stains plant tissue trichomes.
Plant Tissue Culture Tested Powder
Powder CAS Number: 81-88-9
CAS Number: 333-93-7 Formula: C28H31ClN2O3
Formula: C4H12N22HCl Molecular Weight: 479.01
Molecular Weight: 161.1 Soluble in Water

Biochemicals
Soluble in Water Store at Room Temperature
Store at Room Temperature Available Package Sizes
Merck 13, 8038
1g 5g 25g 100g
Available Package Sizes
1g 10g 25g R795 - Ribavirin
1--D-Ribofuranosyl-1H-1,2,4-triazole-3-carboxamide; Virazole
Vitamins Used in Plant Tissue Culture Plant Tissue Culture Tested
See "Figure 13. List of Vitamins used in plant tissue culture Plant Tissue Culture Antiviral
& PhytoTech Product Numbers" on page 239 in the
Powder
Technical Section.
CAS Number: 36791-04-5
P866 - Pyridoxine Hydrochloride Formula: C8H12N4O5
Molecular Weight: 244.2
5-Hydroxy-6-methyl-3,4-pyridinedimethanol Hydrochloride;
Soluble in Water
Vitamin B6 Hydrochloride
Store at 2 to 6 C
Plant Tissue Culture Tested
Merck 13, 8282
Powder
CAS Number: 58-56-0 Available Package Sizes
Formula: C8H11NO3HCl 1g
Molecular Weight: 205.64
Soluble in Water R779 - Riboflavin
Store at Room Temperature USP Grade
Merck 13, 8072 7,8-dimethyl-10-(D-ribo-2,3,4,5-tetrahydroxypentyl)-
isoalloxazine; Vitamin B2
Available Package Sizes
Plant Tissue Culture Tested
25g 100g
Powder
CAS Number: 83-88-5
Formula: C17H20N4O6
Molecular Weight: 376.37
Soluble in KOH
Store at Room Temperature
Merck 13, 8284
Available Package Sizes
25g

Biochemicals A-Z Page 47


Product Catalogue & Laboratory Guide
R7171 - Rose Bengal New Product
See Seed Testing Section for Complete Listing S7530 - Salicylic Acid
SA; 2-Hydroxybenzoic acid.
R7278 - Ruthenium Red
See Seed Testing Section for Complete Listing Salicylic Acid is an endogenous signal mediating local and
systemic plant defense responses against pathogens.
S7775 - Saline Solution (0.85%) Plant Tissue Culture Tested
See Seed Testing Section for Complete Listing
Powder
For More Information on Stains & Dyes CAS Number: 69-72-7
Formula: C7H6O3
See "Figure 35. Stains & Dyes Usage Table" on page 264 in Molecular Weight: 138.12
the Technical Section. Soluble in Ethanol (50mg/mL)
Store at Room Temperature
S7400 - Safranin O
Merck 13, 18411
Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y
or A Available Package Sizes
A biological stain, often used as a counter-stain to Crystal 500g 1Kg
Violet (C1830)
Powder
New Product
CAS Number: 477-73-6 S7580 - Salicylic Acid Sodium Salt
Formula: C20H19ClN4 2-Hydroxybenzoic acid sodium salt; Sodium Salicylate
Molecular Weight: 350.84
Biochemicals

Soluble in Water An endogenous signal mediating local and systemic plant


Store at Room Temperature defense responses against pathogens. Sodium Salicylate is a
Available Package Sizes more water soluble form of Salicylic Acid.
Plant Tissue Culture Tested
25g 100g
Powder
New Product CAS Number: 54-21-7
Formula: C7H5NaO3
S7785 - Methyl Salicylate (MeSA)
Molecular Weight: 160.10
2-Hydroxybenzoic acid methyl ester; Methyl; Soluble in Water (1.25 g/mL)
2-hydroxybenzoate; Wintergreen Oil; MeSA Store at Room Temperature
Merck 13, 8411
Methyl Salicylate is involved in plant defense reactions, present
in plant tissues as well as being an airborne defense signal. Available Package Sizes
Plant Tissue Culture Tested 500g 1Kg
Liquid
CAS Number: 119-36-8
S807 - L-Serine
Formula: C8H8O3 (S)-2-amino-3-hydroxypropanoic acid
Molecular Weight: 152.15 Plant Tissue Culture Tested
Miscible with Water Powder
Store at Room Temperature CAS Number: 56-45-1
Merck 13, 6143 Formula: C3H7O3N
Available Package Sizes Molecular Weight: 105.09
Soluble in Water
500mL 1L Store at Room Temperature
Merck 13, 8534
Available Package Sizes
25g 500g

Page 48 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
S169 - Silver Nitrate S624 - Sodium Chloride
Plant Tissue Culture Tested Plant Tissue Culture Tested
Powder Powder
CAS Number: 7761-88-8 CAS Number: 7647-14-5
Formula: AgNO3 Formula: NaCl
Molecular Weight: 169.89 Molecular Weight: 58.44
Soluble in Water Soluble in Water
Store at Room Temperature Store at Room Temperature
Merck 13, 8591 Merck 13, 8671
Available Package Sizes
500g 1Kg 5Kg
Available Package Sizes
25g 100g S663 - Sodium Citrate Dihydrate
Trisodium Citrate Dihydrate
New Product Plant Tissue Culture Tested
S7220 - Sodium Acetate Trihydrate Powder
Sodium Acetate Trihydrate; Acetic Acid Sodium Salt CAS Number: 6132-04-3
Plant Tissue Culture Tested Formula: C6H5Na3O72H2O
Molecular Weight: 294.1
Powder
Soluble in Water
CAS Number: 6131-90-4
Store at Room Temperature
Formula: C2H3O2Na 3H2O
Merck 13, 8675

Biochemicals
Molecular Weight: 136.10
Soluble in Water Available Package Sizes
Store at Room Temperature 500g 1Kg
Merck 13, 8642
Available Package Sizes S844 - Sodium Dodecyl Sulfate
See Molecular Biology Section for Complete Listing
100g 1Kg

S803 - Sodium Bicarbonate S845 - Sodium Hydroxide


Soda Lye
USP Grade
Plant Tissue Culture Tested
Sodium Hydrogen Carbonate; Baking Soda
Plant Tissue Culture Tested Pellets
CAS Number: 1310-73-2
Powder
Formula: NaOH
CAS Number: 144-55-8
Molecular Weight: 40.00
Formula: NaHCO3
Soluble in Water
Molecular Weight: 84.01
Store at Room Temperature
Soluble in Water
Merck 13, 8701
Store at Room Temperature
Merck 13, 8655 Available Package Sizes
Available Package Sizes 500g 1Kg
25g 500g

S7550 - Sodium Carbonate


See Phycology Section for Complete Listing

Biochemicals A-Z Page 49


Product Catalogue & Laboratory Guide
S835 - Sodium Hydroxide Solution 1.0 N S515 - Sodium Phosphate, Monobasic Monohydrate
Soda Lye Sodium Dihydrogen Phosphate Monohydrate
Sterile Filtered Monosodium Phosphate
Plant Tissue Culture Tested Plant Tissue Culture Tested
Liquid Powder
CAS Number: 1310-73-2 CAS Number: 10049-21-5
Formula: NaOH Formula: NaH2PO4H2O
Molecular Weight: 40.00 Molecular Weight: 137.99
Miscible with Water Soluble in Water
Store at Room Temperature Store at Room Temperature
Merck 13, 8701 Merck 13, 8734
Available Package Sizes
100g 500g 1Kg 5Kg
Available Package Sizes
100mL 500mL 1L 4L S745 - Sodium Phosphate, Dibasic Heptahydrate
Disodium Hydrogen Phosphate Heptahydrate
S7834 - Sodium Metasilicate Disodium Phosphate
See Phycology Section for Complete Listing Plant Tissue Culture Tested
Powder
Micronutrient Sources Chart CAS Number: 7782-85-6
See "Figure 10. List of Micronutrient Sources & PhytoTech Formula: Na2HPO47H2O
Product Numbers" on page 237 in the Technical Section. Molecular Weight: 268.07
Biochemicals

Soluble in Water
M651 - Sodium Molybdate (VI) Dihydrate Store at Room Temperature
Molybdic Acid, Sodium Salt Dihydrate Merck 13, 8733
Plant Tissue Culture Tested Available Package Sizes
Powder 500g 1Kg 5Kg
CAS Number: 10102-40-6
Formula: Na2MoO42H2O S843 - Sodium Sulfate Anhydrous
Molecular Weight: 241.95
Plant Tissue Culture Tested
Soluble in Water
Store at Room Temperature Powder
Merck 13, 8718 CAS Number: 7757-82-6
Formula: Na2SO4
Available Package Sizes Molecular Weight: 142.04
100g 500g Soluble in Water
Store at Room Temperature
S802 - Sodium Nitrate Merck 13, 8755
Plant Tissue Culture Tested Available Package Sizes
Powder 100g 500g
CAS Number: 7631-99-4
Formula: NaNO3 S620 - Sodium Thiosulfate, Anhydrous
Molecular Weight: 84.99
Sodium Hyposulfite
Soluble in Water
Plant Tissue Culture Tested
Store at Room Temperature
Merck 13, 8720 Powder
CAS Number: 7772-98-7
Formula: Na2S2O3
Molecular Weight: 158.11
Available Package Sizes Soluble in Water
500g 1Kg 5Kg Store at Room Temperature
Merck 13, 8769
Available Package Sizes
500g 1Kg

Page 50 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
S744 - D-Sorbitol S7750 - Sudan IV
D-Glucitol Biebrich scarlet R fat soluble, Fat Ponceau R or 4, Lipid
Plant Tissue Culture Tested Crimson, Oil Red IV, Scarlet Red Scharlach, Solvent Red 24,
Powder Sudan R or BB
CAS Number: 50-70-4 Sudan IV is a biological stain used primarily to stain lipids and
Formula: C6H14O6 fatty substances present in cells and tissues.
Molecular Weight: 182.17 Powder
Soluble in Water CAS Number: 85-83-6
Store at Room Temperature Formula: C24H20N4O
Merck 13, 8797 Molecular Weight: 380.44
Soluble in Isopropanol and Chloroform
Available Package Sizes
Store at Room Temperature
500g 1Kg 5Kg 25Kg
Available Package Sizes
S742 - Spectinomycin Dihydrochloride 25g 100g
See Molecular Biology Section for Complete Listing
Carbohydrate Selection Guide
S837 - Spermidine, Free Base See "Figure 12. Choosing a Carbohydrate" on page 238 in the
N-(3-Aminopropyl)-1,4-butanediamine Technical Section.
Plant Tissue Culture Tested
S391 - D-Sucrose
Solid that melts at Room Temperature
CAS Number: 124-20-9 -D-Fructofuranosyl--D-glucopyranoside; D(+)saccharose;
Formula: C7H19N3 Cane Sugar

Biochemicals
Molecular Weight: 145.25 Plant Tissue Culture Tested
Soluble in Water Powder
Store at 2 to 6 C CAS Number: 57-50-1
Merck 13, 8816 Formula: C12H22O11
Molecular Weight: 342.34
Available Package Sizes
Soluble in Water
1g 5g 25g Store at Room Temperature
Merck 13, 8966
S716 - SSC Buffer Solution (20x), pH 7.0
See Molecular Biology Section for Complete Listing Available Package Sizes
30g 500g 1Kg 5Kg 25Kg
S677 - SSPE Buffer Solution (10x)
See Molecular Biology Section for Complete Listing S829 - D-Sucrose, Ultra-Pure
-D-Fructofuranosyl--D-glucopyranoside; D(+)Saccharose;
S739 - Streptomycin Sulfate Cane Sugar
See Molecular Biology Section for Complete Listing Plant Tissue Culture Tested
Powder
S7739 - Streptomycin Sulfate Solution (250 mg/mL) CAS Number: 57-50-1
See Molecular Biology Section for Complete Listing Formula: C12H22O11
Molecular Weight: 342.34
S746 - Succinic Acid 2,2-Dimethylhydrazide Soluble in Water
Daminozide; SADH Store at Room Temperature
Plant Tissue Culture Tested Merck 13, 8966
Powder Available Package Sizes
CAS Number: 1596-84-5 500g 1Kg 5Kg 25Kg
Formula: C6H12N2O3
Molecular Weight: 160.17
Soluble in Water
Store at 2 to 6 C
Merck 13, 2836
Available Package Sizes
10g 100g

Biochemicals A-Z Page 51


Product Catalogue & Laboratory Guide
S804 - Sulfuric Acid Solution 1.0 N T888 - Thidiazuron
Sterile Filtered TDZ; 1-Phenyl-3-(1,2,3-thiadiazol-5-yl)urea
Plant Tissue Culture Tested Plant Tissue Culture Tested
Liquid Purity: minimum 98%
CAS Number: 7664-93-9 Powder
Formula: H2SO4 CAS Number: 51707-55-2
Molecular Weight: 98.07 Formula: C9H8N4OS
Miscible with Water Molecular Weight: 220.3
Store at Room Temperature Soluble in DMSO
Merck 13, 9064 Store at -20 to 0 C
Merck 13, 9384
Available Package Sizes
Available Package Sizes 100mg 500mg 1g
500mL 1L
T438 - Thidiazuron (95%)
S7664 - Sulfuric Acid, 0.1% Solution TDZ; 1-Phenyl-3-(1,2,3-thiadiazol-5-yl)urea
See Phycology Section for Complete Listing Purity: 95%
Plant Tissue Culture Tested
T8199 - Tetrazolium Blue Powder
BTC, Blue Tetrazolium Chloride, TZ CAS Number: 51707-55-2
Tetrazolium Blue is a colorless tetrazolium that forms a colored Formula: C9H8N4OS
formazan when reduced; it is therefore used as an assay of Molecular Weight: 220.3
Biochemicals

reducing sugars. Tetrazolium Blue is used as an indicator for Soluble in DMSO


germination capacity. Store at -20 to 0 C
Powder Merck 13, 9384
CAS Number: 1871-22-3 Available Package Sizes
Formula: C40H32Cl2N8O2
100mg 500mg 1g
Molecular Weight: 727.64
Soluble in Methanol New Product
Store at Room Temperature
T7999 - Thidiazuron Solution (1 mg/mL in DMSO)
Available Package Sizes
TDZ; 1-Phenyl-3-(1,2,3-thiadiazol-5-yl)urea
1g 5g 10g 25g Plant Tissue Culture Tested
Raw Material Purity: Minimum 98%
Vitamins Used in Plant Tissue Culture
Liquid
See "Figure 13. List of Vitamins used in plant tissue culture
CAS Number: 51707-55-2
& PhytoTech Product Numbers" on page 239 in the
Formula: C9H8N4OS
Technical Section.
Molecular Weight: 220.3
T390 - Thiamine Hydrochloride Miscible with Water
Store at -20 to 0 C
3-[(4-Amino-2-methyl-5-pyrimidinyl)methyl]-5-(2- Merck 13, 9384
hydroxyethyl)-4-methylthiazolium chloride Hydrochloride;
Vitamin B1 Hydrochloride
Plant Tissue Culture Tested
Powder Available Package Sizes
CAS Number: 67-03-8 10mL 100mL
Formula: C12H17ClN4OSHCl
Molecular Weight: 337.27 T7982 - Thiourea
Soluble in Water See Seed Testing Section for Complete Listing
Store at Room Temperature
Merck 13, 9366
Available Package Sizes
25g 100g

Page 52 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
T857 - L-Threonine, Methyl Ester, Hydrochloride T7885 - meta-Topolin Solution (1mg/mL)
Plant Tissue Culture Tested 6-(3-Hydroxybenzylamino)purine
Powder Sterile Filtered
CAS Number: 39994-75-7 Plant Tissue Culture Tested
Formula: C5H11O3NHCl Liquid
Molecular Weight: 169.6 CAS Number: 75737-38-1
Soluble in Water Formula: C12H10N5OH
Store at -20 to 0 C Molecular Weight: 241.50
Miscible with Water
Available Package Sizes
Store at -20 to 0 C
5g
Available Package Sizes
T869 - Timentin 10mL 25mL 100mL
See Molecular Biology Section for Complete Listing
T7968 - Trehalose Dihydrate
T767 - Timentin Solution (100 mg/mL) See Seed Testing Section for Complete Listing
See Molecular Biology Section for Complete Listing
T818 - 1-Triacontanol
T7869 - Timentin Solution (50 mg/mL) 1-Hydroxytriacontane; Melissyl Alcohol
See Molecular Biology Section for Complete Listing Plant Tissue Culture Tested
Powder
T8092 - Toluidine Blue O CAS Number: 593-50-0
See Seed Testing Section for Complete Listing

Biochemicals
Formula: C30H62O
Molecular Weight: 438.82
T872 - Tomato Powder Soluble in Hot EtOH
A mixture of natural tomato puree and maltodextrin. Store at 2 to 6 C
Plant Tissue Culture Tested Merck 13, 9665
Powder Available Package Sizes
Soluble in Water
1g
Store at Room Temperature
Available Package Sizes T828 - Trifluralin
500g 1Kg ,,-Trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine
Plant Tissue Culture Tested
T841 - meta-Topolin Powder
6-(3-Hydroxybenzylamino)purine CAS Number: 1582-09-8
Plant Tissue Culture Tested Formula: C13H16F3N3O4
Powder Molecular Weight: 335.28
CAS Number: 75737-38-1 Soluble in DMSO
Formula: C12H10N5OH Store at Room Temperature
Molecular Weight: 241.5 Merck 13, 9757
Soluble in KOH or NaOH Available Package Sizes
Store at -20 to 0 C
100mg 250mg
Available Package Sizes
T850 - 2,3,5-Triiodobenzoic Acid
100mg 500mg 1g
TIBA
Plant Tissue Culture Tested
Powder
CAS Number: 88-82-4
Formula: C7H3O2I3
Molecular Weight: 499.8
Soluble in KOH
Store at -20 to 0 C
Available Package Sizes
5g 10g

Biochemicals A-Z Page 53


Product Catalogue & Laboratory Guide
T761 - Trinexapac-Ethyl T832 - Tryptone
Ethyl 4-cyclopropyl(hydroxyl)methylene-3,5- Enzymatic digest of Casein
dioxocyclohexanecarboxylate Plant Tissue Culture Tested
Plant Tissue Culture Tested Powder
Powder Soluble in Water
CAS Number: 95266-40-3 Store at Room Temperature
Formula: C13H16O5 Available Package Sizes
Molecular Weight: 252.3
Soluble in Water 500g 1Kg
Store at 2 to 6 C
T851 - L-Tryptophan
Available Package Sizes (S)--Amino-1H-indole-3-propanoic Acid
100mg 250mg Plant Tissue Culture Tested
Powder
T8164 - 2,3,5-Triphenyl Tretrazolium Chloride (TTC) CAS Number: 73-22-3
See Seed Testing Section for Complete Listing Formula: C11H12N2O2
Molecular Weight: 204.2
T769 - Tris Acetate EDTA Buffer Solution (50x), Soluble in Water
pH 8.1 0.1 Store at Room Temperature
See Molecular Biology Section for Complete Listing Merck 13, 9868
Available Package Sizes
T838 - Tris Base
100g 500g
See Molecular Biology Section for Complete Listing
Biochemicals

T848 - Tryptose
T831 - Tris Borate EDTA Buffer
Enzymatic Hydrolysate of Protein; Trypticase
See Molecular Biology Section for Complete Listing Plant Tissue Culture Tested
T773 - Tris Borate EDTA Buffer Solution (5x) Powder
Soluble in Water
See Molecular Biology Section for Complete Listing
Store at Room Temperature
T774 - Tris Borate EDTA Buffer Solution (10x) Available Package Sizes
See Molecular Biology Section for Complete Listing 500g

T855 - Tris-EDTA Buffer T8110 - Tyrothricin


See Molecular Biology Section for Complete Listing See Seed Testing Section for Complete Listing

T752 - Tris EDTA Buffer Solution (10X), pH 8.0 T8020 - Tyrothricin Solution (10mg/mL)
See Molecular Biology Section for Complete Listing See Seed Testing Section for Complete Listing

T814 - Tris-Glycine Buffer T873 - L-Tyrosine, Free Base


See Molecular Biology Section for Complete Listing (S)--Amino-4-hydroxybenzenepropanoic Acid
Plant Tissue Culture Tested
T821 - Tris Glycine SDS Buffer
Powder
See Molecular Biology Section for Complete Listing CAS Number: 60-18-4
Formula: C9H11NO3
T749 - Tris-Glycine-SDS Buffer Solution (10x) Molecular Weight: 181.21
See Molecular Biology Section for Complete Listing Soluble in HCl; Slightly soluble in water
Store at Room Temperature
T858 - Tris Hydrochloride Merck 13, 9907
See Molecular Biology Section for Complete Listing
Available Package Sizes
T764 - Tris HCl Solution 1.0 M, pH 7.5 5g 100g 500g 1Kg
See Molecular Biology Section for Complete Listing

Page 54 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
U890 - Urea Y892 - Yeast Extract
Carbonyldiamide; Carbamide Microbiological Grade
Plant Tissue Culture Tested Plant Tissue Culture Tested
Powder Powder
CAS Number: 57-13-6 CAS Number: 8013-01-2
Formula: CH4N2O Soluble in Water
Molecular Weight: 60.06 Store at 2 to 6 C
Soluble in Water Available Package Sizes
Store at Room Temperature
Merck 13, 9935 500g 1Kg 2.5Kg

Available Package Sizes Making Stock Solutions of Plant Growth


500g 1Kg 5Kg Regulators
See "Figure 16. Stock Solution Dilution Chart" on page 242
V878 - L-Valine in the Technical Section.
(S)-2-amino-3-methylbutanoic Acid
Plant Tissue Culture Tested Z125 - Zeatin, trans Isomer
Powder (2E)-2-methyl-4-(1H-purin-6-ylamino)-2-buten-1-ol
CAS Number: 72-18-4 Plant Tissue Culture Tested
Formula: C5H11NO2 Powder
Molecular Weight: 117.17 CAS Number: 1637-39-4
Soluble in Water Formula: C10H13N5O
Store at Room Temperature

Biochemicals
Molecular Weight: 219.25
Merck 13, 9975 Soluble in KOH
Available Package Sizes Store at -20 to 0 C
Merck 13, 10170
25g 100g
Available Package Sizes
V886 - Vinegar, White Distilled 10mg 50mg 100mg 250mg 500mg 1g
5% Acetic Acid
Liquid Z875 - Zeatin Riboside Solution (1 mg/mL), trans Isomer
CAS Number: 64-19-7 9-(-D-Ribofuranosyl)-trans-zeatin, N6-(trans-4-Hydroxy-3-
Formula: C2H4O2 methyl-2-buten-1-yl)adenosine
Molecular Weight: 60.06 Aqueous Solution
Miscible with Water Sterile Filtered
Store at Room Temperature Plant Tissue Culture Tested
Available Package Sizes Liquid
CAS Number: 6025-53-2
15mL
Formula: C15H21N5O5
X874 - X-Gal Molecular Weight: 351.4
Miscible with Water
See Molecular Biology Section for Complete Listing
Store at -20 to 0 C
X877 - X-Gluc, Monocyclohexyl Ammonium Salt Available Package Sizes
See Molecular Biology Section for Complete Listing 10mL

X871 - X-Gluc, Sodium Salt


See Molecular Biology Section for Complete Listing

X8451 - X-Gluc Solution (20mg/mL)


See Molecular Biology Section for Complete Listing

Biochemicals A-Z Page 55


Product Catalogue & Laboratory Guide
Z899 - Zeatin Riboside, trans Isomer Z897 - Zinc Nitrate Hexahydrate
9-(-D-Ribofuranosyl)-trans-zeatin, N6-(trans-4-Hydroxy-3- Plant Tissue Culture Tested
methyl-2-buten-1-yl)adenosine Powder
Plant Tissue Culture Tested CAS Number: 10196-18-6
Powder Formula: Zn(NO3)26H2O
CAS Number: 6025-53-2 Molecular Weight: 297.48
Formula: C15H21N5O5 Soluble in Water
Molecular Weight: 351.4 Store at Room Temperature
Soluble in KOH Merck 13, 10197
Store at -20 to 0 C
Available Package Sizes
10mg 50mg 100mg 250mg 1g Available Package Sizes
100g 500g 1Kg
Z860 - Zeatin Solution (1 mg/mL), trans Isomer
(2E)-2-methyl-4-(1H-purin-6-ylamino)-2-buten-1-ol Z101 - Zinc Sulfate Heptahydrate
Sterile Filtered Plant Tissue Culture Tested
Plant Tissue Culture Tested Powder
Liquid CAS Number: 7446-20-0
CAS Number: 1637-39-4 Formula: ZnSO47H2O
Formula: C10H13N5O Molecular Weight: 287.54
Molecular Weight: 219.25 Soluble in Water
Miscible with Water Store at Room Temperature
Biochemicals

Store at -20 to 0 C Merck 13, 10213


Merck 13, 10170
Available Package Sizes
Available Package Sizes 100g 500g
10mL

Find Microbiology Media on Pages 151 to 159


LB Agars and Broths
Tryptic Soy Broth
YEP Media
Hanahans Broth
Peptone Waters

Page 56 2015 PhytoTechnology Laboratories


Product Catalogue & Laboratory Guide
Plant Tissue Culture Media Featured in this section
PhytoTechnology Laboratories offers Agronomic Media
a large selection of ready-to-use plant
tissue culture media. The media outlined
in this section are for plant tissue Crop Specific Media
micropropagation. Media for other
applications, please refer to the following
sections: Deficient Media
Microbiology, page 151
Phytopathology, page 161
Seed Testing, page 165
MS Media
Phycology, page 173
Orchid Media
The formulation for each non-proprietary
Plant Tissue Culture Media

media is detailed in the product listings


on the following pages. To compare Stock Solutions
media formulations, please consult the
formulation tables in the technical section
(pages 270 to 293) or visit Woody Plant Media
www.phytotechlab.com.

For instructions on media preparation and


Vitamins
usage please consult "Powdered Media" on
page 232 or visit www.phytotechlab.com. Murashige & Skoog
Formulations
For assistance locating products within Many of the media formulations offered
this catalogue, please consult the indices by PhytoTechnology Laboratories are
at the back of this catalogue. Be sure to derivatives of the formulation popularized
check back at www.phytotechlab.com for by Toshio Murashige and Folke Skoog in
new products! 1962. On the following page is provided
a breakdown of the MS formulations
Custom Media Formulations offered by PhytoTechnology Laboratories
In addition to the many popular by media types.
formulations offered, PhytoTechnology
Laboratories also offers custom Our Product Definitions
manufacturing services for proprietary Basal Salts contain macronutrients
research formulations. Custom and micronutrients. Does not include
formulations provide all of the benefits vitamins, plant growth regulators, or
of pre-made media but can be tailored carbohydrates.
to specific research needs. Eliminate Basal Media contains macronutrients
variability from batch to batch by and micronutrients, as well as some
taking advantage of custom media organic components. Generally lacks
manufacturing. All custom media meets either vitamins, plant growth regulators,
ISO 9001:2008 and cGMP standards of or carbohydrates, and is therefore not
quality. considered a "complete medium".
Media typically considered complete
See "Custom Services" on page 12 for and requires only gelling agents, if desired.
more information about Custom Media.

Page 58 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
Murashige & Skoog Media Family

Media with
Deficient Media
Vitamins & PGRs
M502 - MS Macronutrient Salt Base
M516 - MS BC Potato Medium
M654 - MS Macronutrient Stock Solution
M401 - MS Modified Medium
M554 - MS Micronutrient Salt Base
M550 - MS Modified Medium with Kinetin & 2,4-D
M529 - MS Micronutrient Stock Solution (10x)
M701 - MS Modified Medium with 2iP, IAA, & Adenine
M407 - MS Basal Salts without N, P, & K
M702 - MS Modified Medium with 2iP, IAA, & Adenine
M561 - MS Basal Mixture with 1/2 NH4NO3 & KNO3
M555 - MS Modified Multiplication Medium with Kinetin
M571 - MS Basal Salt Mixture without NH4NO3
M517 - MS African Violet/Gloxinia Multiplication Medium
M531 - MS Basal Salt Mixture without Nitrogen
M518 - MS African Violet/Gloxinia Pretransplant Medium
M290 - MS Modified Basal Salt Mixture with 1/2 NH4NO3, KNO3, &
M508 - MS Fern Multiplication Medium with Kinetin & NAA
CaCl
M509 - MS Gerbera Multiplication Medium with Kinetin & IAA
M153 - MS Basal Salt Mixture with 1/2 Macro- & Micronutrients
M510 - MS Gerbera Pretransplant Medium with IAA
M511 - MS Kalanoche Multiplication Medium with 2iP
M512 - MS Kalanoche Pretransplant Medium with IAA

Plant Tissue Culture Media


M527 - MS Multiplication Medium with Kinetin & IAA
Basal Salts M491 - MS Multiplication Medium with 2iP & IAA
M507 - Murashige Cattleya Orchid Medium with IAA, IBA, & NAA

M524 - MS Basal Salt Mixture


M576 - MS Basal Salt Concentrate Solution (20x) Crop Specific
M489 - MS Basal Salts with FeNaEDTA
M5541 - MS Basal Salt Mixture, Van Der Salm Modification Media
M504 - MS Basal Salts, Finer & Nagasawa Modification
M153 - MS Basal Salt Mixture with 1/2 Macro- & Micronutrients
M516 - MS BC Potato Medium
M517 - MS African Violet/Gloxinia Multiplication Medium
Media with M518 - MS African Violet/Gloxinia Pretransplant Medium
Vitamins M508 - MS Fern Multiplication Medium with Kinetin & NAA
M509 - MS Gerbera Multiplication Medium with Kinetin & IAA
M510 - MS Gerbera Pretransplant Medium with IAA
L689 - Linsmaier & Skoog (LS) Medium M511 - MS Kalanoche Multiplication Medium with 2iP
M5800 - MS Medium with FeNaEDTA M512 - MS Kalanoche Pretransplant Medium with IAA
M541 - MS Modified Medium with Casein & Adenine; without KH2PO4 B144 - Banana AGS Medium
M535 - MS Modified Medium with Adenine M462 - Musa (Banana) Multiplication Medium
M536 - MS Modified Medium with Adenine M507 - Murashige Cattleya Orchid Medium with IAA, IBA, & NAA
M5642 - MS Medium, Van Der Salm Modification H435 - Hosta Initiation/Multiplication Medium
M519 - MS Medium with Vitamins H436 - Hosta Multiplication Medium
M404 - MS Medium with Gamborg's Vitamins H437 - Hosta Pretransplant Medium
M5531 - MS Medium with 1.0 g/L MES H3959 - Hosta Initiation/Multiplication Medium II
L477 - LS Medium with 1.0 g/L MES B468 - BY-2 Medium (for tobacco)

Media with
Vitamins & Sucrose
M5615- -MS
M5615 MSMedium
Mediumwith
with15.0
15.0g/L
g/LSucrose
Sucrose&&Glucose
Glucose
M5501- -MS
M5501 MSMedium
Mediumwith
withVitamins
Vitaminsand&Sucrose
Sucrose Murashige & Skoog Media
M5530- -MS
M5530 MSMedium
Mediumwith
Solution (1x) with Vitamins & Sucrose
Sucrose Toshio Murashige and Folke Skoog developed the
M5707- -MS
M5707 MSMedium
Mediumwith
with10.0
10.0g/L
g/LSucrose
Sucroseand
& 20.0 g/L Glucose original formulation of Murashige & Skoog Medium for
L473- -LSLSMedium
L473 Mediumwith
with30.0
30.0g/L
g/LSucrose
Sucroseand
&1.0
1.0mg/L
mg/LMES
MES
use with tobacco callus cultures in 1962. The basal salts
and vitamins described, have become the most popular
Media with Vitamins, plant tissue culture media. MS Medium is effective on
a wide variety of plants and there are many popular
Sucrose & Agar modifications designed for specific plants or growth
L467 - LS Medium with 30.0 g/L Sucrose & 7.0 g/L Agar
requirements.
L452 - LS Medium with 30.0 g/L Sucrose, 7.0 g/L Agar, & 1.0 g/L MES
The original formulations are:
M524 - MS Basal Salt Mixture
M519 - MS Medium with Vitamins

Plant Tissue Culture Media A-Z Page 59


Product Catalogue & Laboratory Guide
A267 - Anderson Basal Salt Mixture B144 - Banana AGS Basal Medium
Contains the macro- and micronutrients as described by Contains the macro- and micronutrients, vitamins, and plant
Anderson (1978, 1980). growth regulators required to culture bananas.
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water Soluble in Water
Use at 1.89 grams per liter Use at 4.71 grams per liter
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Nitrate 400 Ammonium Nitrate 1650
Boric Acid 6.2 Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2 Calcium Chloride, Anhydrous 333
Cobalt Chloride6H2O 0.025 Cobalt Chloride6H2O 0.025
Cupric Sulfate5H2O 0.025 Cupric Sulfate5H2O 0.025
Na2EDTA2H2O 74.5 Ferric Sodium EDTA 36.7
Plant Tissue Culture Media

Ferrous Sulfate7H2O 55.7 Magnesium Sulfate, Anhydrous 181


Magnesium Sulfate, Anhydrous 180.7 Manganese SulfateH2O 16.9
Manganese SulfateH2O 16.9 Molybdic Acid, Disodium Salt2H2O 0.25
Molybdic Acid, Disodium Salt2H2O 0.25 Potassium Iodide 0.83
Potassium Iodide 0.3 Potassium Nitrate 1900
Potassium Nitrate 480 Potassium Phosphate, Monobasic, Anhydrous 170
Sodium Phosphate, MonobasicH2O 330.6 Sodium Phosphate, MonobasicH2O 295
Zinc Sulfate7H2O 8.6 Zinc Sulfate7H2O 8.6
Approximate pH at Room Temperature 3.75 0.5 6-(,-Dimethylallylamino)purine 10
Available Package Sizes Indole-3-acetic Acid 1.0
1L 10L 50L myo-Inositol 100
ThiamineHCl 0.4
B129 - Bacteria Screening Medium 523 Approximate pH at Room Temperature 4.75 0.5
See Phytopathology Section for Complete Listing
Available Package Sizes
1L 10L 50L

BG-11 Blue Green Media


See Phycology Section for Complete Listings

Find Biochemicals on Pages 15 to 56


Gelling Agents
Plant Growth Regulators
Media Components
Stains and Dyes
Carbohydrates

Page 60 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
B514 - Blaydes Basal Medium B138 - BM-1 Terrestrial Orchid Medium
Contains the macro- and micronutrients, sucrose, and Without Agar
thiamine as described by Blaydes (1966). Contains the macro- and micronutrients, vitamins, and
Store at 2 to 6 C supplements required to culture orchids.
Soluble in Water Especially suited for terrestrial orchids, Paphiopedilum, and
Use at 31.86 grams per liter Phragmipedium.
Plant Tissue Culture Tested Seed germination may be enhanced by the addition of 50 mL/L
Components (mg/L) Coconut Water (Product Number C195).
Ammonium Nitrate 1000 Store at 2 to 6 C
Soluble in Water
Boric Acid 1.6 Use at 21.22 grams per liter
Calcium Nitrate 241.1 Plant Tissue Culture Tested
Na2EDTA2H2O 74.5 Components (mg/L)
Ferrous Sulfate7H2O 55.7 Boric Acid 10
Magnesium Sulfate, Anhydrous 17.1 Cobalt Chloride6H2O 0.025

Plant Tissue Culture Media


Manganese SulfateH2O 4.4 Cupric Sulfate5H2O 0.025
Potassium Chloride 100 Na2EDTA2H2O 37.25
Potassium Iodide 0.8 Ferrous Sulfate7H2O 27.85
Potassium Nitrate 65 Magnesium Sulfate, Anhydrous 100
Potassium Phosphate, Monobasic, Anhydrous 300 Manganese SulfateH2O 25
Zinc Sulfate7H2O 1.5 Molybdic Acid, Disodium Salt2H2O 0.25
Glycine 2.0 Potassium Phosphate, Monobasic, Anhydrous 300
Sucrose 30,000 Zinc Sulfate7H2O 10
ThiamineHCl 0.1 D-Biotin 0.05
Approximate pH at Room Temperature 3.5 0.75 Casein, Enzymatic Hydrolysate 500
Available Package Sizes Folic Acid 0.5
1L 10L 50L L-Glutamine 100
Glycine 2.0
myo-Inositol 100
Nicotinic Acid 5.0
PyridoxineHCl 0.5
Sucrose 20,000
ThiamineHCl 0.5
Tissue Culture Kits
Approximate pH at Room Temperature 5.5 0.75
PhytoTechnology Laboratories
offers a selection of plant tissue Available Package Sizes
culture kits for educational 1L 10L 50L
use. For more information
please see pages 179 to 185. Terrestrial Orchid Media Selection Guide
See "Figure 22. Terrestrial Orchid Media Selection Guide" on
Kits for: page 250 in the Technical Section.
African Violet,
Carrot,
Carnivorous Plants,
Fern,
Hosta,
Lily,
Orchids, and
Potato.

Plant Tissue Culture Media A-Z Page 61


Product Catalogue & Laboratory Guide
B141 - BM-1 Terrestrial Orchid Medium With Agar B470 - BM-2 Terrestrial Orchid Medium
Contains the macro- and micronutrients, vitamins, and Without Agar
supplements required to culture orchids. Contains 0.2 mg/L 6-Benzylaminopurine (BA, Product
Especially suited for terrestrial orchids, Paphiopedilum, and Number B800).
Phragmipedium. Contains the macro- and micronutrients, vitamins, and
Seed germination may be enhanced by the addition of 50 mL/L supplements required to culture orchids.
Coconut Water (Product Number C195).
Store at 2 to 6 C
Store at 2 to 6 C Soluble in Water
Soluble in Water (Partially), Fully Soluble in Use at 21.22 grams per liter
Boiling Water Plant Tissue Culture Tested
Use at 26.22 grams per liter
Components (mg/L)
Plant Tissue Culture Tested
Boric Acid 10
Components (mg/L)
Cobalt Chloride6H2O 0.025
Boric Acid 10
Cupric Sulfate5H2O 0.025
Cobalt Chloride6H2O 0.025
Na2EDTA2H2O 37.25
Plant Tissue Culture Media

Cupric Sulfate5H2O 0.025


Ferrous Sulfate7H2O 27.85
Na2EDTA2H2O 37.25
Magnesium Sulfate, Anhydrous 100
Ferrous Sulfate7H2O 27.85
Manganese SulfateH2O 25
Magnesium Sulfate, Anhydrous 100
Molybdic Acid, Disodium Salt2H2O 0.25
Manganese SulfateH2O 25
Potassium Phosphate, Monobasic, Anhydrous 300
Molybdic Acid, Disodium Salt2H2O 0.25
Zinc Sulfate7H2O 10
Potassium Phosphate, Monobasic, Anhydrous 300
6-Benzylaminopurine 0.2
Zinc Sulfate7H2O 10
D-Biotin 0.05
Agar 5000
Casein, Enzymatic Hydrolysate 500
D-Biotin 0.05
Folic Acid 0.5
Casein, Enzymatic Hydrolysate 500
L-Glutamine 100
Folic Acid 0.5
Glycine 2.0
L-Glutamine 100
myo-Inositol 100
Glycine 2.0
Nicotinic Acid 5.0
myo-Inositol 100
PyridoxineHCl 0.5
Nicotinic Acid 5.0
Sucrose 20,000
PyridoxineHCl 0.5
ThiamineHCl 0.5
Sucrose 20,000
Approximate pH at Room Temperature 5.25 0.5
ThiamineHCl 0.5
Approximate pH at Room Temperature 5.5 0.75 Available Package Sizes
1L 10L 50L
Available Package Sizes
1L 10L 50L

Tissue Culture & Orchids


See "Tissue Culture & Orchids" on page 250 to 251 in the
Technical Section.

Page 62 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
B142 - BM-2 Terrestrial Orchid Medium With Agar B1396 - Broadleaf Tree Basal Medium
Contains 0.2 mg/L 6-Benzylaminopurine (BA, Product Contains the micro- and macro-nutrients and vitamins
Number B800). described by Chalupa (1984).
Contains the macro- and micronutrients, vitamins, and Store at 2 to 6 C
supplements required to culture orchids. Soluble in Water
Especially suited for terrestrial orchids, Paphiopedilum and Use at 1.21 grams per liter
Phragmipedium. Plant Tissue Culture Tested
Store at 2 to 6 C Components (mg/L)
Soluble in Water (Partially), Fully Soluble in Boiling Water
Use at 27.22 grams per liter Ammonium Nitrate 82.5
Plant Tissue Culture Tested Ammonium Sulfate 120
Components (mg/L) Boric Acid 1.55
Boric Acid 10 Calcium Chloride, Anhydrous 16.6
Cobalt Chloride6H2O 0.025 Calcium Nitrate 222
Cupric Sulfate5H2O 0.025 Cobalt Chloride, Hexahydrate 0.005

Plant Tissue Culture Media


Na2EDTA2H2O 37.25 Cupric Sulfate5H2O 0.063
Ferrous Sulfate7H2O 27.85 Na2EDTA2H2O 9.325
Magnesium Sulfate, Anhydrous 100 Ferrous Sulfate7H2O 6.95
Manganese SulfateH2O 25 Magnesium Sulfate, Anhydrous 90
Molybdic Acid, Disodium Salt2H2O 0.25 Manganese SulfateH2O 4.225
Potassium Phosphate, Monobasic, Anhydrous 300 Molybdic Acid, Disodium Salt2H2O 0.063
Zinc Sulfate7H2O 10 Potassium Iodide 0.038
Agar 6000 Potassium Nitrate 95
6-Benzylaminopurine 0.2 Potassium Phosphate, Monobasic, Anhydrous 85
D-Biotin 0.05 Potassium Sulfate, Anhydrous 430
Casein, Enzymatic Hydrolysate 500 Zinc Nitrate6H2O 2.15
Folic Acid 0.5 myo-Inositol 50
L-Glutamine 100 Nicotinic Acid 0.25
Glycine 2.0 Approximate pH at Room Temperature 4.0 0.5
myo-Inositol 100 Available Package Sizes
Nicotinic Acid 5.0 1L 10L 50L
PyridoxineHCl 0.5
Sucrose 20,000
ThiamineHCl 0.5
Approximate pH at Room Temperature 5.5 0.5
Available Package Sizes
1L 10L 50L

B1650 - Bolds Basal Media (BBM)


See Phycology Section for Complete Listings

Plant Tissue Culture Media A-Z Page 63


Product Catalogue & Laboratory Guide
C206 - Cape Sundew/Venus Flytrap C216 - Cape Sundew/Venus Flytrap
Multiplication Basal Medium Pretransplant Basal Medium
Contains the macro- and micronutrients, vitamins, and plant Contains the macro- and micronutrients, vitamins, and plant
growth regulators required to culture Cape Sundew and Venus growth regulators required to culture Cape Sundew and Venus
Flytrap. Flytrap.
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water Soluble in Water
Use at 2.12 grams per liter Use at 2.20 grams per liter
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Nitrate 400 Ammonium Nitrate 825
Boric Acid 6.2 Boric Acid 3.1
Calcium Chloride, Anhydrous 332.2 Calcium Chloride, Anhydrous 166.5
Cobalt Chloride6H2O 0.025 Cobalt Chloride6H2O 0.0125
Cupric Sulfate5H2O 0.025 Cupric Sulfate5H2O 0.0125
Plant Tissue Culture Media

Na2EDTA2H2O 74.5 Ferric Sodium EDTA 18.35


Ferrous Sulfate7H2O 55.7 Magnesium Sulfate, Anhydrous 90.5
Magnesium Sulfate, Anhydrous 180.7 Manganese SulfateH2O 8.45
Manganese SulfateH2O 16.9 Molybdic Acid, Disodium Salt2H2O 0.125
Molybdic Acid, Disodium Salt2H2O 0.25 Potassium Iodide 0.415
Potassium Nitrate 480 Potassium Nitrate 950
Sodium Phosphate, MonobasicH2O 380 Potassium Phosphate, Monobasic, Anhydrous 85
Zinc Sulfate7H2O 8.6 Zinc Sulfate7H2O 4.3
Adenine Hemisulfate2H2O 80 myo-Inositol 50
6-(,-Dimethylallylamino)purine 1.0 ThiamineHCl 0.2
myo-Inositol 100 Approximate pH at Room Temperature 5.0 0.5
ThiamineHCl 0.4 Available Package Sizes
Approximate pH at Room Temperature 3.75 0.5 1L 10L 50L
Available Package Sizes
1L 10L 50L

Find Molecular Biology Products on Pages 131 to 150


Antibiotics
Buffers
DNA Extraction Kits
Sterile Solutions
IPTG, X-Gal & X-Gluc

Page 64 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
C1935 - Carnivorous Plant (Nepenthes) Basal C212 - Carrot Callus Initiation Basal Medium
Salt Mixture Contains the macro- and micronutrients, vitamins, and plant
This medium is commonly supplemented with MS Vitamins growth regulators required to initiate carrot callus from root
(e.g., M533, M553) and 25 g/L sucrose (Product Number tissue.
S391). Store at 2 to 6 C
Store at 2 to 6 C Soluble in Water
Soluble in Water Use at 3.21 grams per liter
Use at 0.65 grams per liter Plant Tissue Culture Tested
Plant Tissue Culture Tested Components (mg/L)
Components (mg/L) Ammonium Sulfate 134
Ammonium Nitrate 160.1 Boric Acid 3.0
Boric Acid 9.275 Calcium Chloride, Anhydrous 113.24
Calcium Chloride, Anhydrous 33.94 Cobalt Chloride6H2O 0.025
Cobalt Chloride6H2O 0.024 Cupric Sulfate5H2O 0.025
Cupric Sulfate5H2O 0.025 Na2EDTA2H2O 37.26

Plant Tissue Culture Media


Na2EDTA2H2O 36.72 Ferrous Sulfate7H2O 27.8
Magnesium Sulfate, Anhydrous 36.11 Magnesium Sulfate, Anhydrous 122.09
Manganese SulfateH2O 16.90 Manganese SulfateH2O 10
Molybdic Acid, Disodium Salt2H2O 0.242 Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Iodide 0.830 Potassium Iodide 0.75
Potassium Nitrate 202.2 Potassium Nitrate 2500
Sodium Phosphate, Monobasic 138.01 Sodium Phosphate, MonobasicH2O 150
Zinc Sulfate7H2O 11.502 Zinc Sulfate7H2O 2.0
Approximate pH at Room Temperature 4.5 0.5 2,4-Dichlorophenoxyacetic Acid 1.0
Available Package Sizes myo-Inositol 100
1L 10L 50L Nicotinic Acid 1.0
PyridoxineHCl 1.0
ThiamineHCl 10
Approximate pH at Room Temperature 4.0 0.5
Available Package Sizes
1L 10L 50L

Plant Tissue Culture Media A-Z Page 65


Product Catalogue & Laboratory Guide
C222 - Carrot Shoot Development Basal Medium C287 - Chee & Pool C2d Vitis Basal Medium
Contains the macro- and micronutrients, vitamins, and plant Contains the macro- and micronutrients and vitamins as
growth regulators required to initiate shoots from carrot callus. described by Chee & Pool (1987).
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water Soluble in Water
Use at 3.21 grams per liter Use at 4.49 grams per liter
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Sulfate 134 Ammonium Nitrate 1650
Boric Acid 3.0 Boric Acid 6.2
Calcium Chloride, Anhydrous 113.24 Calcium Nitrate 492.3
Cobalt Chloride6H2O 0.025 Cobalt Chloride6H2O 0.025
Cupric Sulfate5H2O 0.025 Cupric Sulfate5H2O 0.025
Na2EDTA2H2O 37.26 Na2EDTA2H2O 37.3
Plant Tissue Culture Media

Ferrous Sulfate7H2O 27.8 Ferrous Sulfate7H2O 27.8


Magnesium Sulfate, Anhydrous 122.09 Magnesium Sulfate, Anhydrous 180.6
Manganese SulfateH2O 10 Manganese SulfateH2O 0.845
Molybdic Acid, Disodium Salt2H2O 0.25 Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Iodide 0.75 Potassium Nitrate 1900
Potassium Nitrate 2500 Potassium Phosphate, Monobasic, Anhydrous 170
Sodium Phosphate, MonobasicH2O 150 Zinc Sulfate7H2O 8.6
Zinc Sulfate7H2O 2.0 myo-Inositol 10
myo-Inositol 100 Nicotinic Acid 1.0
Kinetin 0.2 PyridoxineHCl 1.0
Nicotinic Acid 1.0 ThiamineHCl 1.0
PyridoxineHCl 1.0 Approximate pH at Room Temperature 4.0 0.5
ThiamineHCl 10 Available Package Sizes
Approximate pH at Room Temperature 4.0 0.5 1L 10L 50L
Available Package Sizes
C149 - Chu N6 Vitamin Solution (1000x)
1L 10L 50L
Contains the vitamins as described by Chu et al. (1975).
Store at 2 to 6 C
Sterile Filtered
Miscible with Water
Use at 1.0 mL per liter of medium
Plant Tissue Culture Tested
Components (mg/L)
Glycine 2000
Nicotinic Acid 500
PyridoxineHCI 500
ThiamineHCI 1000
Approximate pH at Room Temperature 4.0 0.5
Available Package Sizes
100mL

Page 66 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
C167 - Chu N6 Basal Medium with Vitamins C442 - Corn Meal Agar
Contains the macro- and micronutrients and vitamins as See Phytopathology Section for Complete Listing
described by Chu et al. (1975).
Store at 2 to 6 C
Czapek-Dox Broth & Agar
Soluble in Water See Phytopathology Section for Complete Listings
Use at 3.99 grams per liter
Plant Tissue Culture Tested D146 - DCR Basal Salt Mixture
Components (mg/L) Contains the macro- and micronutrients as described by Gupta
& Durzan (1985).
Ammonium Sulfate 463
Store at 2 to 6 C
Boric Acid 1.6
Soluble in Water
Calcium Chloride, Anhydrous 125.33 Use at 1.64 grams per liter
Na2 EDTA2H2O 37.25 Plant Tissue Culture Tested
Ferrous Sulfate7H2O 27.85 Components (mg/L)
Magnesium Sulfate, Anhydrous 90.37 Ammonium Nitrate 400

Plant Tissue Culture Media


Manganese SulfateH2O 3.3 Boric Acid 6.2
Potassium Iodide 0.8 Calcium Chloride, Anhydrous 64.14
Potassium Nitrate 2830 Calcium Nitrate 386.31
Potassium Phosphate, Monobasic, Anhydrous 400 Cobalt Chloride6H2O 0.025
Zinc Sulfate7H2O 1.5 Cupric Sulfate5H2O 0.25
Glycine 2.0 Na2EDTA2H2O 37.3
Nicotinic Acid 0.5 Ferrous Sulfate7H2O 27.8
PyridoxineHCl 0.5 Magnesium Sulfate, Anhydrous 180.7
ThiamineHCl 1.0 Manganese SulfateH2O 22.3
Approximate pH at Room Temperature 4.0 0.5 Molybdic Acid, Disodium Salt2H2O 0.25
Available Package Sizes Nickel Chloride6H2O 0.025
1L 10L 10LFB 50L 100L Potassium Iodide 0.83
Potassium Nitrate 340
C416 - Chu N6 Basal Salt Mixture Potassium Phosphate, Monobasic, Anhydrous 170
Contains the macro- and micronutrients as described by Chu et Zinc Sulfate7H2O 8.6
al. (1975).
Approximate pH at Room Temperature 4.0 0.5
Store at 2 to 6 C
Soluble in Water Available Package Sizes
Use at 3.98 grams per liter 1L 10L 50L
Plant Tissue Culture Tested
Components (mg/L)
Ammonium Sulfate 463
Boric Acid 1.6
Calcium Chloride, Anhydrous 125.33
Na2 EDTA2H2O 37.25
Ferrous Sulfate7H2O 27.85
Magnesium Sulfate, Anhydrous 90.37
Manganese SulfateH2O 3.3
Potassium Iodide 0.8
Potassium Nitrate 2830
Potassium Phosphate, Monobasic, Anhydrous 400
Zinc Sulfate7H2O 1.5
Approximate pH at Room Temperature 4.25 0.5
Available Package Sizes
1L 10L 10LFB 50L 100L

Plant Tissue Culture Media A-Z Page 67


Product Catalogue & Laboratory Guide
D2206 - De Greef & Jacobs Medium D191 - DKW Basal Medium
Contains the macro- and micronutrients and vitamins as Contains 10 g/L Sucrose; without vitamins.
described by De Greef & Jacobs (1979). Contains the macro- and micronutrients as described by Driver
Store at 2 to 6 C & Kuniyuki (1984) and McGranahan et al. (1987).
Soluble in Water Store at 2 to 6 C
Use at 3.91 grams per liter of medium Soluble in Water
Plant Tissue Culture Tested Use at 15.22 grams per liter
Components (mg/L) Plant Tissue Culture Tested
Ammonium Sulfate 400 Note: The high calcium level in this medium may inhibit many
gellan gums from completely melting during autoclaving. For this
Boric Acid 10.62
reason the use of Gellan Gum or Agargellan with this medium is
Calcium Chloride, Anhydrous 226.5 not recommended as a clear, firm gel may not be obtained.
Cobalt Chloride 0.0025 Components (mg/L)
Cupric Sulfate 0.0025 Ammonium Nitrate 1416
Ferrous Sulfate 27.8 Boric Acid 4.8
Plant Tissue Culture Media

Magnesium Sulfate, anhydrous 244.33 Calcium Chloride, Anhydrous 112.5


Manganese Sulfate, monohydrate 1.68 Calcium Nitrate 1367
Disodium EDTA, dihydrate 37.26 Cupric Sulfate5H2O 0.25
Potassium Chloride 600 Na2EDTA2H2O 45.4
Potassium Iodide 1.58 Ferrous Sulfate7H2O 33.8
Potassium Nitrate 2000 Magnesium Sulfate, Anhydrous 361.49
Sodium Phosphate, monobasic 250 Manganese SulfateH2O 33.5
Sodium Molybdate 0.0025 Molybdic Acid, Disodium Salt2H2O 0.39
Zinc Sulfate 1.06 Nickel Sulfate6H2O 0.005
myo-Inositol 100 Potassium Phosphate, Monobasic, Anhydrous 265
Nicotinic Acid 1 Potassium Sulfate, Anhydrous 1559
Pyridoxine HCl 1 Zinc Nitrate6H2O 17
Thiamine HCl 10 Sucrose 10,000
Approximate pH at Room Temperature 4.0 0.5 Approximate pH at Room Temperature 4.0 0.5
Available Package Sizes Available Package Sizes
1L 10L 50L 1L 10L 50L

Find Seed Testing Products on Pages 165 to 172


PhytoSelect Basal Medium
mD5A Medium
Stains & Dyes
Kings B Medium
Selection Agents

Page 68 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
D189 - DKW Basal Medium D190 - DKW Basal Salt Mixture
Contains 30 g/L Sucrose; without vitamins. Contains the macro- and micronutrients as described by Driver
Contains the macro- and micronutrients as described by Driver & Kuniyuki (1984) and McGranahan et al. (1987).
& Kuniyuki (1984) and McGranahan et al. (1987). Store at 2 to 6 C
Store at 2 to 6 C Soluble in Water
Soluble in Water Use at 5.22 grams per liter
Use at 35.22 grams per liter Plant Tissue Culture Tested
Plant Tissue Culture Tested Note: The high calcium level in this medium may inhibit many
Note: The high calcium level in this medium may inhibit many gellan gums from completely melting during autoclaving. For this
gellan gums from completely melting during autoclaving. For this reason the use of Gellan Gum or Agargellan with this medium is
reason the use of Gellan Gum or Agargellan with this medium is not recommended as a clear, firm gel may not be obtained.
not recommended as a clear, firm gel may not be obtained. Components (mg/L)
Components (mg/L) Ammonium Nitrate 1416
Ammonium Nitrate 1416 Boric Acid 4.8
Boric Acid 4.8 Calcium Chloride, Anhydrous 112.5

Plant Tissue Culture Media


Calcium Chloride, Anhydrous 112.5 Calcium Nitrate 1367
Calcium Nitrate 1367 Cupric Sulfate5H2O 0.25
Cupric Sulfate5H2O 0.25 Na2EDTA2H2O 45.4
Na2EDTA2H2O 45.5 Ferrous Sulfate7H2O 33.8
Ferrous Sulfate7H2O 33.8 Magnesium Sulfate, Anhydrous 361.49
Magnesium Sulfate, Anhydrous 361.49 Manganese SulfateH2O 33.5
Manganese SulfateH2O 33.5 Molybdic Acid, Disodium Salt2H2O 0.39
Molybdic Acid, Disodium Salt2H2O 0.39 Nickel Sulfate6H2O 0.005
Nickel Sulfate6H2O 0.005 Potassium Phosphate, Monobasic, Anhydrous 265
Potassium Phosphate, Monobasic, Anhydrous 265 Potassium Sulfate, Anhydrous 1559
Potassium Sulfate, Anhydrous 1559 Zinc Nitrate6H2O 17
Zinc Nitrate6H2O 17 Approximate pH at Room Temperature 4.0 0.5
Sucrose 30,000
Available Package Sizes
Approximate pH at Room Temperature 4.0 0.5
1L 10L 50L
Available Package Sizes
1L 10L 50L

Find Phycology Products on Pages 173 to 178


Bolds Basal Medium
Blue-Green Medium
Tris Acetate Phosphate
Seawater
Sueokas High Salt Medium

Plant Tissue Culture Media A-Z Page 69


Product Catalogue & Laboratory Guide
D2470 - DKW Basal Medium with Vitamins E575 - Economou & Read Basal Medium
Contains the macro- and micronutrients as described by Driver Contains the macro- and micronutrients and vitamins as
& Kuniyuki (1984) and McGranahan et al. (1987). described by Economou & Read (1984).
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water Soluble in Water
Use at 5.32 grams per liter Use at 1.84 grams per liter
Plant Tissue Culture Tested Plant Tissue Culture Tested
Note: The high calcium level in this medium may inhibit many Components (mg/L)
gellan gums from completely melting during autoclaving. For this
Ammonium Nitrate 400
reason the use of Gellan Gum or Agargellan with this medium is
not recommended as a clear, firm gel may not be obtained. Ammonium Sulfate 132
Components (mg/L) Boric Acid 6.2
Ammonium Nitrate 1416 Calcium Chloride, Anhydrous 332.2
Boric Acid 4.8 Cobalt Chloride6H2O 0.025
Calcium Chloride, Anhydrous 112.5 Cupric Sulfate5H2O 0.025
Plant Tissue Culture Media

Calcium Nitrate 1367 Iron Chelate, Sequestrene 330 56


Cupric Sulfate5H2O 0.25 Magnesium Sulfate, Anhydrous 180.7
Na2EDTA2H2O 45.4 Manganese SulfateH2O 16.9
Ferrous Sulfate7H2O 33.8 Molybdic Acid, Disodium Salt2H2O 0.25
Magnesium Sulfate, Anhydrous 361.49 Potassium Nitrate 202
Manganese SulfateH2O 33.5 Potassium Phosphate, Monobasic, Anhydrous 408
Molybdic Acid, Disodium Salt2H2O 0.39 Zinc Sulfate7H2O 8.6
Nickel Sulfate6H2O 0.005 myo-Inositol 100
Potassium Phosphate, Monobasic, Anhydrous 265 ThiamineHCI 0.4
Potassium Sulfate, Anhydrous 1559 Approximate pH at Room Temperature 4.5 0.5
Zinc Nitrate6H2O 17 Available Package Sizes
myo-Inositol 100 1L 10L 50L
Glycine 2
E330 - Eriksson Vitamin Solution (1000x)
Nicotinic Acid 1
Contains the vitamins as described by Eriksson (1965).
ThiamineHCl 2
Store at 2 to 6 C
Approximate pH at Room Temperature 4.0 0.5 Sterile Filtered
Available Package Sizes Miscible with Water
1L 10L 50L Use at 1.0 mL per liter of medium
Plant Tissue Culture Tested
Components (mg/L)
Glycine 2000
Nicotinic Acid 500
PyridoxineHCl 500
ThiamineHCl 500
Approximate pH at Room Temperature 4.25 0.5
Available Package Sizes
100mL

Page 70 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
E333 - EXS IIIa Basal Medium with Adenine F522 - Fast Terrestrial Orchid Medium
Contains Adenine Hemisulfate (Product Number A545) and Contains the macro- and micronutrients, vitamins, sucrose and
vitamins. Does not contain carbohydrates or gelling agents. fructose supplements required to culture terrestrial orchids.
EXS IIIa is a proprietary plant cell culture medium that Contains Agar.
was originally developed for the culture of foliage plants Store at 2 to 6 C
such as Syngonium, Ficus and Spathiphyllum. Soluble in Water (Partially)
Store at 2 to 6 C Use at 27.84 grams per liter
Soluble in Water Plant Tissue Culture Tested
Use at 3.95 grams per liter Components (mg/L)
Plant Tissue Culture Tested
Aluminum Chloride 0.03
Approximate pH at Room Temperature 4.0 0.5
Ammonium Nitrate 167
Available Package Sizes Boric Acid 1
1L 10L 50L
Calcium Nitrate 40.1
E337 - EXS III Basal Medium without Adenine Cupric Sulfate5H2O 0.03

Plant Tissue Culture Media


Contains vitamins. Without Adenine Hemisulfate. Does not FeNaEDTA 17
contain carbohydrates or gelling agents. Ferric ChlorideH2O 1
EXS III is a proprietary plant cell culture medium that was
Magnesium Sulfate, Anhydrous 19.8
originally developed for the culture of foliage plants such as
Syngonium, Ficus and Spathiphyllum. Manganese SulfateH2O 0.8
Store at 2 to 6 C Nickle Chloride6H2O 0.03
Soluble in Water Potassium Chloride 167
Use at 3.87 grams per liter Potassium Iodide 0.01
Plant Tissue Culture Tested
Potassium Phosphate, Dibasic 83
Approximate pH at Room Temperature 4.75 0.5
Zinc Sulfate7H2O 1
Available Package Sizes Agar 7000
1L 10L 50L D-Biotin 0.01
f/2 Guillards Marine Enriched Seawaters Fructose 5000
See Phycology Section for Complete Listings Nicotinic Acid 0.1
Peptone from Meat 1670
Sucrose 11,670
Yeast Extract 2000
Approximate pH at Room Temperature 6.5 0.5
Available Package Sizes
Custom Media & Services 1L 10L 50L
PhytoTechnology Laboratories
offers custom manufacturing Selecting Terrestrial Orchid Media by Genus
and testing services. For more See "Figure 24. Terrestrial Orchid Media Selection Guide by
information please see page Genus" on page 251 in the Technical Section.
12.

Custom Dry Powder Media

Custom Liquid Media

Custom Packaging

Testing Services

Plant Tissue Culture Media A-Z Page 71


Product Catalogue & Laboratory Guide
G398 - Gamborg B-5 Basal Medium G768 - Gamborg Basal Salt Mixture (B-5 Salts)
Contains the macro- and micronutrients and vitamins as Contains the macro- and micronutrients as described by
described by Gamborg et al. (1968). Gamborg et al. (1968).
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water Soluble in Water
Use at 3.21 grams per liter Use at 3.10 grams per liter
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Sulfate 134 Ammonium Sulfate 134
Boric Acid 3.0 Boric Acid 3.0
Calcium Chloride, Anhydrous 113.24 Calcium Chloride, Anhydrous 113.24
Cobalt Chloride6H2O 0.025 Cobalt Chloride6H2O 0.025
Cupric Sulfate5H2O 0.025 Cupric Sulfate5H2O 0.025
Na2 EDTA2H2O 37.26 Na2 EDTA2H2O 37.26
Plant Tissue Culture Media

Ferrous Sulfate7H2O 27.8 Ferrous Sulfate7H2O 27.8


Magnesium Sulfate, Anhydrous 122.09 Magnesium Sulfate, Anhydrous 122.09
Manganese SulfateH2O 10 Manganese SulfateH2O 10
Molybdic Acid, Disodium Salt2H2O 0.25 Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Iodide 0.75 Potassium Iodide 0.75
Potassium Nitrate 2500 Potassium Nitrate 2500
Sodium Phosphate, MonobasicH2O 150 Sodium Phosphate, MonobasicH2O 150
Zinc Sulfate7H2O 2.0 Zinc Sulfate7H2O 2.0
myo-Inositol 100 Approximate pH at Room Temperature 4.0 0.5
Nicotinic Acid 1.0 Available Package Sizes
PyridoxineHCl 1.0 1L 10L 10LFB 50L
ThiamineHCl 10
Approximate pH at Room Temperature 4.0 0.5
G249 - Gamborg Vitamin Powder (1000x)
Contains the vitamins as described by Gamborg et al. (1968).
Available Package Sizes myo-Inositol may precipitate out of the prepared stock solution
1L 10L 10LFB 50L 100L when cold; warming with occasional agitation will redissolve
the precipitated myo-Inositol.
Store at 2 to 6 C
Soluble in Water
Use 11.20 grams to make 100 mL of 1000x stock
Use at 1.0 mL per liter of medium
Plant Tissue Culture Tested
Components (mg/L)
myo-Inositol 100,000
Nicotinic Acid (Free Acid) 1000
PyridoxineHCl 1000
ThiamineHCl 10,000
Approximate pH at Room Temperature 3.25 0.5
Available Package Sizes
100 mL

Page 72 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
G219 - Gamborg Vitamin Solution (1000x) G371 - Gresshoff & Doy Basal Medium
Contains the vitamins as described by Gamborg et al. (1968). Contains the macro- and micronutrients and vitamins as
myo-Inositol may precipitate out of the prepared stock solution described by Gresshoff & Doy (1974).
when cold; warming with occasional agitation will redissolve Store at 2 to 6 C
the precipitated myo-Inositol. Soluble in Hot Water
Store at 2 to 6 C Use at 2.71 grams per liter
Sterile Filtered Plant Tissue Culture Tested
Miscible with Water Components (mg/L)
Use at 1.0 mL per liter of medium
Ammonium Nitrate 1000
Plant Tissue Culture Tested
Boric Acid 0.3
Components (mg/L)
Calcium Nitrate 241.2
myo-Inositol 100,000
Cobalt Chloride6H2O 0.025
Nicotinic Acid 1000
Cupric Sulfate5H2O 0.025
PyridoxineHCl 1000
Na2EDTA2H2O 37.25
ThiamineHCl 10,000

Plant Tissue Culture Media


Ferrous Sulfate7H2O 27.85
Approximate pH at Room Temperature 3.5 0.75
Magnesium Sulfate, Anhydrous 17.1
Available Package Sizes
Manganese SulfateH2O 1.0
100mL 500mL
Molybdic Acid, Disodium Salt2H2O 0.025
Vitamins Mixtures for use in Plant Tissue Culture Potassium Chloride 65
See "Vitamin Formulations Table" on page 287 in the Potassium Iodide 0.8
Technical Section. Potassium Nitrate 1000
Potassium Phosphate, Monobasic, Anhydrous 300
Zinc Sulfate7H2O 0.3
D-Biotin 0.2
Glycine 4.0
myo-Inositol 10
Nicotinic Acid 0.1
PyridoxineHCl 0.1
ThiamineHCl 1.0
Find Culture Vessels in the Equipment Section Approximate pH at Room Temperature 4.0 0.5
For our full line of Available Package Sizes
culture vessels, consult
the equipment section 1L 10L 50L
on pages 187 to 218.
H289 - Hanahans Broth
Sterile Culture Vessels See Microbiology Section for Complete Listing

Reusable Culture
Vessels

Petri Dishes

Culture Tubes

Plant Tissue Culture Media A-Z Page 73


Product Catalogue & Laboratory Guide
O612 - H1 Oat Medium H393 - Heller Basal Salt Mixture
Contains the macro- and macronutrients and supplements Contains the macro- and micronutrients as described by Heller
required for the symbiotic culture of orchids. Contains Agar (1953).
and Sucrose. Store at 2 to 6 C
Store at 2 to 6 C Soluble in Water
Soluble in Water (Partially) Use at 1.64 grams per liter
Use at 12.52 grams per liter Plant Tissue Culture Tested
Plant Tissue Culture Tested Components (mg/L)
Components (mg/L) Aluminum Chloride6H2O 0.0543
Calcium Nitrate 96.6 Boric Acid 1.0
Magnesium Sulfate 23.9 Calcium Chloride, Anhydrous 56.7
Potassium Chloride 100 Cupric Sulfate5H2O 0.03
Potassium Phosphate, Monobasic 200 Ferric Chloride6H2O 1.0
Agar 7000 Magnesium Sulfate, Anhydrous 122.1
Plant Tissue Culture Media

Sucrose 2000 Manganese SulfateH2O 0.0758


Rolled Oats 3000 Molybdenum Trioxide 0.03
Yeast Extract 100 Potassium Chloride 750
Approximate pH at Room Temperature 6.0 1.0 Potassium Iodide 0.01
Available Package Sizes Sodium Nitrate 600
1L 10L Sodium Phosphate, MonobasicH2O 108.75
Zinc Sulfate7H2O 1.0
O622 - H2 Oat Medium Approximate pH at Room Temperature 5.0 0.5
Contains the macro- and micronutrients and supplements
required for the symbiotic culture of orchids. Contains Agar Available Package Sizes
and Glucose. 1L 10L 50L
Store at 2 to 6 C
H353 - Hoagland Modified Basal Salt Mixture
Soluble in Water (Partially)
Use at 12.52 grams per liter Contains Ferrous Sulfate
Plant Tissue Culture Tested Contains the macro- and micronutrients as described by
Hoagland & Arnon (1950).
Components (mg/L)
Store at 2 to 6 C
Calcium Nitrate 96.6
Soluble in Water
Magnesium Sulfate 23.9 Use at 1.63 grams per liter
Potassium Chloride 100 Plant Tissue Culture Tested
Potassium Phosphate, Monobasic 200 Components (mg/L)
Agar 7000 Ammonium Phosphate, Monobasic 115.03
Glucose 2000 Boric Acid 2.86
Rolled Oats 3000 Calcium Nitrate 656.4
Yeast Extract 100 Cupric Sulfate5H2O 0.08
Approximate pH at Room Temperature 6.0 1.0 Na2EDTA2H2O 3.35
Available Package Sizes Ferrous Sulfate7H2O 2.50
1L 10L Magnesium Sulfate, Anhydrous 240.76
Manganese Chloride4H2O 1.81
Molybdenum Trioxide 0.016
Potassium Nitrate 606.6
Zinc Sulfate7H2O 0.22
Approximate pH at Room Temperature 4.75 0.5
Available Package Sizes
1L 10L 50L 100L

Page 74 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
For Help Preparing Tissue Culture Media H435 - Hosta Initiation/ Multiplication Medium
See "Figure 7. Basic Steps to Preparing 1 Liter of Medium" on Stage I/II Medium
page 233 in the Technical Section. Contains the macro- and micronutrients as described by
Murashige & Skoog (1962) and the vitamins described by
H3959 - Hosta Initiation/Multiplication Medium II Linsmaier & Skoog (1965). Also contains sucrose and agar.
A modification of the macro- and micronutrients of Murashige Store at 2 to 6 C
and Skoog Basal Medium (M524) with vitamins, PGRs, Soluble in Water (Partially), Fully Soluble in Boiling/Hot
Sucrose and Agar. Water
Store at 2 to 6 C Use at 43.40 grams per liter
Partially soluble in Water Plant Tissue Culture Tested
Use at 43.4 grams per liter of medium Components (mg/L)
Plant Tissue Culture Tested
Ammonium Nitrate 1650
Components (mg/L) Boric Acid 6.2
6-Benzylaminopurine 2 Calcium Chloride, Anhydrous 332.2
Adenine Hemisulfate 160 Cobalt Chloride6H2O 0.025

Plant Tissue Culture Media


Agar 8000 Cupric Sulfate5H2O 0.025
Ammonium Nitrate 1650 Na2EDTA2H2O 37.26
-Naphthaleneacetic Acid 0.02 Ferrous Sulfate7H2O 27.8
Boric Acid 6.2 Magnesium Sulfate, Anhydrous 180.7
Calcium Chloride, Anhydrous 332.2 Manganese SulfateH2O 16.9
Casein Hydrolysate 500 Molybdic Acid, Disodium Salt2H2O 0.25
Cobalt Chloride6H2O 0.025 Potassium Iodide 0.83
Cupric Sulfate5H2O 0.025 Potassium Nitrate 1900
Ferrous Sulfate7H2O 27.8 Potassium Phosphate, Monobasic, Anhydrous 300
Glycine 2 Sodium Phosphate, MonobasicH2O 170
Magnesium Sulfate, Anhydrous 180.7 Zinc Sulfate7H2O 8.6
Manganese SulfateH2O 16.9 Adenine Hemisulfate2H2O 160
Molybdic Acid, Disodium Salt2H2O 0.25 Agar 8000
myo-Inositol 100 6-Benzylaminopurine 2.0
Na2EDTA2H2O 37.26 Casein, Enzymatic Hydrolysate 500
Potassium Iodide 0.83 Glycine 2.0
Potassium Nitrate 1900 myo-Inositol 100
Potassium Phosphate, Monobasic, Anhydrous 300 -Naphthaleneacetic Acid 0.5
Sodium Phosphate, MonobasicH2O 170 Sucrose 30,000
Sucrose 30,000 ThiamineHCl 0.4
ThiamineHCl 0.4 Approximate pH at Room Temperature 4.5 0.75
Zinc Sulfate7H2O 8.6 Available Package Sizes
Approximate pH at Room Temperature 4.5 0.75 1L 10L 50L
Available Package Sizes
1L 10L 50L

Plant Tissue Culture Media A-Z Page 75


Product Catalogue & Laboratory Guide
H436 - Hosta Multiplication Medium H437 - Hosta Rooting Medium
Stage II Medium Stage III Medium
Contains the macro- and micronutrients as described by Contains the macro- and micronutrients as described by
Murashige & Skoog (1962) and the vitamins described by Murashige & Skoog (1962) and the vitamins described by
Linsmaier & Skoog (1965). Also contains Sucrose and Agar. Linsmaier & Skoog (1965). Also contains Sucrose and Agar.
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water (Partially), Fully Soluble in Boiling/Hot Soluble in Water (Partially), Fully Soluble in Boiling/Hot
Water Water
Use at 43.39 grams per liter Use at 43.23 grams per liter
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Nitrate 1650 Ammonium Nitrate 1650
Boric Acid 6.2 Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2 Calcium Chloride, Anhydrous 332.2
Cobalt Chloride6H2O 0.025 Cobalt Chloride6H2O 0.025
Plant Tissue Culture Media

Cupric Sulfate5H2O 0.025 Cupric Sulfate5H2O 0.025


Na2EDTA2H2O 37.26 Na2EDTA2H2O 37.26
Ferrous Sulfate7H2O 27.8 Ferrous Sulfate7H2O 27.8
Magnesium Sulfate, Anhydrous 180.7 Magnesium Sulfate, Anhydrous 180.7
Manganese SulfateH2O 16.9 Manganese SulfateH2O 16.9
Molybdic Acid, Disodium Salt2H2O 0.25 Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Iodide 0.83 Potassium Iodide 0.83
Potassium Nitrate 1900 Potassium Nitrate 1900
Potassium Phosphate, Monobasic, Anhydrous 300 Potassium Phosphate, Monobasic, Anhydrous 300
Sodium Phosphate, MonobasicH2O 170 Sodium Phosphate, MonobasicH2O 170
Zinc Sulfate7H2O 8.6 Zinc Sulfate7H2O 8.6
Adenine Hemisulfate2H2O 160 Agar 8000
Agar 8000 6-Benzylaminopurine 0.1
6-Benzylaminopurine 0.1 Casein, Enzymatic Hydrolysate 500
Casein, Enzymatic Hydrolysate 500 myo-Inositol 100
Glycine 2.0 -Naphthaleneacetic Acid 0.5
myo-Inositol 100 Sucrose 30,000
-Naphthaleneacetic Acid 0.5 ThiamineHCl 0.4
Sucrose 30,000 Approximate pH at Room Temperature 5.0 0.75
ThiamineHCl 0.4 Available Package Sizes
Approximate pH at Room Temperature 4.5 0.75 1L 10L 50L
Available Package Sizes
1L 10L 50L

Page 76 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
I365 - Ichihashi New Phalaenopsis (NP) Medium K427 - Kao & Michayluk Modified Basal Medium
Contains the components as described by Ichihashi (1992); Contains the macro- and micronutrients, vitamins, and organic
modified to contain 82.0 mg/L Ammonium Nitrate (Product supplements as described by Kao & Michayluk (1975).
Number A300). Store at 2 to 6 C
Store at 2 to 6 C Soluble in Water
Soluble in Water (Partially), Fully Soluble in Boiling/Hot Use at 3.90 grams per liter
Water Plant Tissue Culture Tested
Use at 25.35 grams per liter Components (mg/L)
Plant Tissue Culture Tested
Ammonium Nitrate 600
Components (mg/L)
Boric Acid 3.0
Ammonium Nitrate 82
Calcium Chloride, Anhydrous 453
Ammonium Sulfate 303.9
Cobalt Chloride6H2O 0.025
Boric Acid 3.1
Cupric Sulfate5H2O 0.025
Calcium Nitrate 637.6
Na2EDTA2H2O 37.26
Cobalt Chloride6H2O 0.0125

Plant Tissue Culture Media


Ferrous Sulfate7H2O 27.85
Cupric Sulfate5H2O 0.0125
Magnesium Sulfate, Anhydrous 146.55
Na2EDTA2H2O 37.3
Manganese SulfateH2O 10
Ferrous Sulfate7H2O 27.8
Molybdic Acid, Disodium Salt2H2O 0.25
Magnesium Nitrate6H2O 256.4
Potassium Chloride 300
Manganese SulfateH2O 11.2
Potassium Iodide 0.75
Molybdic Acid, Disodium Salt2H2O 0.125
Potassium Nitrate 1900
Potassium Iodide 0.415
Potassium Phosphate, Monobasic, Anhydrous 170
Potassium Nitrate 424
Zinc Sulfate7H2O 2.0
Potassium Phosphate, Monobasic, Anhydrous 462.7
p-Aminobenzoic Acid 0.02
Zinc Sulfate7H2O 4.3
L-Ascorbic Acid 2.0
Gellan Gum, CultureGelTM Biotech 3000
D-Biotin 0.01
Glycine 2.0
Calcium Pantothenate 1.0
myo-Inositol 100
Cholecalciferol; Vitamin D3 0.01
Nicotinic Acid 0.5
Choline Chloride 1.0
PyridoxineHCl 0.5
Citric Acid, Anhydrous 40
Sucrose 20,000
Cyanocobalamin; Vitamin B12 0.02
ThiamineHCl 0.1
Folic Acid 0.4
Approximate pH at Room Temperature 4.75 0.5
Fumaric Acid, Free Acid 40
Available Package Sizes Malic Acid 40
1L 10L 50L myo-Inositol 100
Molecular Biology Kits Niacinamide 1.0
PhytoTechnology Laboratories offers a variety PyridoxineHCl 1.0
of kits for molecular biology. Please see the Pyruvic Acid, Potassium Salt 20
molecular biology section on pages 131 to Retinol; Vitamin A 0.01
150 for more information. Riboflavin 0.2
RNA Extraction Kits ThiamineHCl 1.0
Approximate pH at Room Temperature 3.5 0.75
Genomic DNA Extraction Kits
Available Package Sizes
Plasmid DNA Extraction Kits 1L 10L 50L 100L
DNA Ladder Kits

Plant Tissue Culture Media A-Z Page 77


Product Catalogue & Laboratory Guide
K413 - Kao & Michayluk Basal Salt Mixture K421 - Kao & Michayluk Vitamin Solution
Contains the macro- and micronutrients as described by Kao & (100x)
Michayluk (1975). Contains the vitamins as described by Kao & Michayluk
Store at 2 to 6 C (1975).
Soluble in Water Store at -20 to 0 C
Use at 3.65 grams per liter Sterile Filtered
Plant Tissue Culture Tested Miscible with Water
Components (mg/L) Use at 10.0 mL per liter of Medium
Ammonium Nitrate 600 Plant Tissue Culture Tested
Boric Acid 3.0 Components (mg/L)
Calcium Chloride, Anhydrous 453 p-Aminobenzoic Acid 2.0
Cobalt Chloride6H2O 0.025 L-Ascorbic Acid 200
Cupric Sulfate5H2O 0.025 D-Biotin 1.0
Na2 EDTA2H2O 37.26 Calcium Pantothenate 100
Choline Chloride 100
Plant Tissue Culture Media

Ferrous Sulfate7H2O 27.85


Magnesium Sulfate, Anhydrous 146.55 Cyanocobalamin; Vitamin B12 2.0
Manganese SulfateH2O 10 Folic Acid 40
Molybdic Acid, Disodium Salt2H2O 0.25 myo-Inositol 10,000
Potassium Chloride 300 Niacinamide 100
Potassium Iodide 0.75 PyridoxineHCl 100
Potassium Nitrate 1900 Retinol; Vitamin A 1.0
Potassium Phosphate, Monobasic, Anhydrous 170 Riboflavin 20
Zinc Sulfate7H2O 2.0 ThiamineHCl 100
Approximate pH at Room Temperature 4.0 0.5 Approximate pH at Room Temperature 5.25 1.75

Available Package Sizes Available Package Sizes


1L 10L 50L 100mL 500mL 1L

K5013 - Kings B Medium


See Seed Testing Section for Complete Listing

Custom Media & Services


PhytoTechnology Laboratories
offers custom manufacturing
and testing services. For more
information please see page
12.

Custom Dry Powder Media

Custom Liquid Media

Custom Packaging

Testing Services

Page 78 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
K400 - Knudson C Orchid Medium Morel L154 - Lloyd & McCown Woody Plant Basal
Modification Salt Mixture
Contains the components as described by Knudson (1946) as Contains the macro- and micronutrients as described by Lloyd
modified by Morel (1965). & McCown (1981).
For optimal results, this medium may be supplemented with Store at 2 to 6 C
5-10% (v/v) Cocount Water (Product Number C195) or 30-50 Soluble in Water
g/L Banana Powder (Product Number B852). Use at 2.30 grams per liter
Store at 2 to 6 C Plant Tissue Culture Tested
Soluble in Water Components (mg/L)
Use at 22.00 grams per liter
Ammonium Nitrate 400
Plant Tissue Culture Tested
Boric Acid 6.2
Components (mg/L)
Calcium Chloride, Anhydrous 72.5
Ammonium Nitrate 500
Calcium Nitrate 386
Ammonium Sulfate 500
Cupric Sulfate5H2O 0.25
Calcium Nitrate 347.2

Plant Tissue Culture Media


Na2EDTA2H2O 37.3
Ferrous Sulfate7H2O 25
Ferrous Sulfate7H2O 27.85
Magnesium Sulfate, Anhydrous 122.13
Magnesium Sulfate, Anhydrous 180.7
Manganese SulfateH2O 5.68
Manganese SulfateH2O 22.3
Potassium Chloride 250
Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Phosphate, Monobasic, Anhydrous 250
Potassium Phosphate, Monobasic, Anhydrous 170
Sucrose 20,000
Potassium Sulfate, Anhydrous 990
Approximate pH at Room Temperature 4.5 0.5
Zinc Sulfate7H2O 8.6
Available Package Sizes
Approximate pH at Room Temperature 4.0 0.5
1L 10L 50L
Available Package Sizes
Epiphytic Orchid Media Selection Guide 1L 10L 50L 100L
See "Figure 23. Epiphytic Orchid Media Selection Guide" on
page 250 in the Technical Section.

K425 - Knudson C Modified Plus Orchid Medium


Proprietary Formulation
A complete orchid replate and seed sowing medium.
Contains Activated Charcoal, Sucrose, Banana Powder,
and a Gelling Agent.
Store at 2 to 6 C
Soluble in Water (Partially), Fully Soluble in Hot/Boiling
Water
Use at 79.11 grams per liter
Plant Tissue Culture Tested
Approximate pH at Room Temperature 4.75 0.5
Available Package Sizes
1L 10L 50L

LB Broths & Agars


See Microbiology Section for Complete Listings

L476 - Leifert & Waites Sterility Test Medium


See Phytopathology Section for Complete Listing

Plant Tissue Culture Media A-Z Page 79


Product Catalogue & Laboratory Guide
L472 - Lindemann Orchid Basal Medium L467 - Linsmaier & Skoog Modified Basal Medium
Contains the macro- and micronutrients as described by with 30 g/L Sucrose & 7 g/L Agar
Lindemann et al. (1970); contains Sucrose and Vitamins. Contains the macro- and micronutrients and vitamins as
A complete orchid replate and seed sowing medium. described by Linsmaier & Skoog (1965). Also contains
Store at 2 to 6 C 30 g/L Sucrose and 7 g/L Agar.
Soluble in Water (Partially), Fully Soluble in Warm to Hot This medium may be sold as Murashige & Skoog (MS)
Water Medium with Minimal Organics (MSMO) by some media
Use at 22.71 grams per liter suppliers.
Plant Tissue Culture Tested Store at 2 to 6 C
Components (mg/L) Soluble in Water (Partially), Fully Soluble in
Aluminum Chloride6H2O 0.0561 Hot/Boiling Water
Use at 41.43 grams per liter
Ammonium Sulfate 1000
Plant Tissue Culture Tested
Boric Acid 1.014
Components (mg/L)
Calcium Nitrate 347.2
Ammonium Nitrate 1650
Cupric Sulfate5H2O 0.019
Plant Tissue Culture Media

Boric Acid 6.2


Ferric Citrate 4.4
Calcium Chloride, Anhydrous 332.2
Magnesium Sulfate, Anhydrous 58.62
Cobalt Chloride6H2O 0.025
Manganese SulfateH2O 0.0515
Cupric Sulfate5H2O 0.025
Nickel Chloride6H2O 0.0312
Na2EDTA2H2O 37.26
Potassium Chloride 1050
Ferrous Sulfate7H2O 27.8
Potassium Iodide 0.099
Magnesium Sulfate, Anhydrous 180.7
Potassium Phosphate, Monobasic, Anhydrous 135
Manganese SulfateH2O 16.9
Zinc Sulfate7H2O 0.565
Molybdic Acid, Disodium Salt2H2O 0.25
Glycine 2.0
Potassium Iodide 0.83
myo-Inositol 100
Potassium Nitrate 1900
Nicotinic Acid 1.0
Potassium Phosphate, Monobasic, Anhydrous 170
PyridoxineHCl 1.0
Zinc Sulfate7H2O 8.6
Sucrose 20,000
Agar 7000
ThiamineHCl 10
myo-Inositol 100
Approximate pH at Room Temperature 4.5 0.5
Sucrose 30,000
Available Package Sizes ThiamineHCl 0.4
1L 10L 50L Approximate pH at Room Temperature 4.75 0.5
Available Package Sizes
1L 10L 50L

Page 80 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
L452 - Linsmaier & Skoog Modified Basal Medium L689 - Linsmaier & Skoog Basal Medium
with 30 g/L Sucrose & 7 g/L Agar Contains the macro- and micronutrients and vitamins as
pH Adjusted and Buffered described by Linsmaier & Skoog (1965).
Contains the macro- and micronutrients and vitamins as This medium may be sold as Murashige & Skoog (MS)
described by Linsmaier & Skoog (1965). Also contains Minimal Organics (MSMO) by some media suppliers.
30 g/L Sucrose and 7 g/L Agar. Store at 2 to 6 C
This medium may be sold as Murashige & Skoog (MS) Soluble in Water
Medium with Minimal Organics (MSMO) by some media Use at 4.43 grams per liter
suppliers. Plant Tissue Culture Tested
Store at 2 to 6 C Components (mg/L)
Soluble in Water (Partially), Fully Soluble in Hot/Boiling Ammonium Nitrate 1650
Water
Boric Acid 6.2
Use at 42.53 grams per liter
Plant Tissue Culture Tested Calcium Chloride, Anhydrous 332.2
Components (mg/L) Cobalt Chloride6H2O 0.025
Ammonium Nitrate 1650 Cupric Sulfate5H2O 0.025

Plant Tissue Culture Media


Boric Acid 6.2 Na2EDTA2H2O 37.26
Calcium Chloride, Anhydrous 332.2 Ferrous Sulfate7H2O 27.8
Cobalt Chloride6H2O 0.025 Magnesium Sulfate 180.7
Cupric Sulfate5H2O 0.025 Manganese SulfateH2O 16.9
Na2EDTA2H2O 37.26 Molybdic Acid, Disodium Salt2H2O 0.25
Ferrous Sulfate7H2O 27.8 Potassium Iodide 0.83
Magnesium Sulfate 180.7 Potassium Nitrate 1900
Manganese SulfateH2O 16.9 Potassium Phosphate, Monobasic, Anhydrous 170
Molybdic Acid, Disodium Salt2H2O 0.25 Zinc Sulfate7H2O 8.6
Potassium Hydroxide 100 myo-Inositol 100
Potassium Iodide 0.83 ThiamineHCl 0.4
Potassium Nitrate 1900 Approximate pH at Room Temperature 4.0 0.5
Potassium Phosphate, Monobasic, Anhydrous 170 Available Package Sizes
Zinc Sulfate7H2O 8.6 1L 10L 10LFB 50L 100L
Agar 7000
myo-Inositol 100
MES (Free Acid) H2O 1000
Sucrose 30,000
ThiamineHCl 0.4
Approximate pH at Room Temperature 5.75 0.5
Available Package Sizes
1L 10L 50L

Plant Tissue Culture Media A-Z Page 81


Product Catalogue & Laboratory Guide
L473 - Linsmaier & Skoog Modified Basal Medium L477 - Linsmaier & Skoog Modified Basal Medium
with 30 g/L Sucrose pH Adjusted and Buffered
pH Adjusted and Buffered Contains the macro- and micronutrients and vitamins as
Contains the macro- and micronutrients and vitamins as described by Linsmaier & Skoog (1965).
described by Linsmaier & Skoog (1965). This medium may This medium may be sold as Murashige & Skoog (MS)
be sold as Murashige & Skoog (MS) Medium with Minimal Medium with Minimal Organics (MSMO) by some media
Organics (MSMO) by some media suppliers. suppliers.
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water Soluble in Water
Use at 35.53 grams per liter Use at 5.53 grams per liter
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Nitrate 1650 Ammonium Nitrate 1650
Boric Acid 6.2 Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2 Calcium Chloride, Anhydrous 332.2
Plant Tissue Culture Media

Cobalt Chloride6H2O 0.025 Cobalt Chloride6H2O 0.025


Cupric Sulfate5H2O 0.025 Cupric Sulfate5H2O 0.025
Na2EDTA2H2O 37.26 Na2EDTAH2O 37.26
Ferrous Sulfate7H2O 27.8 Ferrous Sulfate7H2O 27.8
Magnesium Sulfate, Anhydrous 180.7 Magnesium Sulfate, Anhydrous 180.7
Manganese SulfateH2O 16.9 Manganese SulfateH2O 16.9
Molybdic Acid, Disodium Salt2H2O 0.25 Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Hydroxide 100 Potassium Hydroxide 100
Potassium Iodide 0.83 Potassium Iodide 0.83
Potassium Nitrate 1900 Potassium Nitrate 1900
Potassium Phosphate, Monobasic, Anhydrous 170 Potassium Phosphate, Monobasic, Anhydrous 170
Zinc Sulfate7H2O 8.6 Zinc Sulfate7H2O 8.6
myo-Inositol 100 myo-Inositol 100
MES (Free Acid)H2O 1000 MESH2O 1000
Sucrose 30,000 ThiamineHCl 0.4
ThiamineHCl 0.4 Approximate pH at Room Temperature 5.75 0.5
Approximate pH at Room Temperature 5.75 0.5 Available Package Sizes
Available Package Sizes 1L 10L 50L
1L 10L 50L

Page 82 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
L546 - Litvay Basal Salt Mixture L444 - Lloyd & McCown Woody Plant
Contains the macro- and micronutrients as described by Litvay Micronutrient Mixture
et al. (1981). Contains the micronutrients as described by Lloyd &
Originally developed for the culture of both juvenile and McCown (1981).
mature tissues of Douglas-fir (Pseudotsuga menziesii) and
Store at 2 to 6 C
Loblolly pine (Pinus taeda).
Soluble in Water
Store at 2 to 6 C Use at 0.53 grams per liter
Soluble in Water Plant Tissue Culture Tested
Use at 4.95 grams per liter
Components (mg/L)
Plant Tissue Culture Tested
Boric Acid 6.2
Components (mg/L)
Calcium Chloride, Anhydrous 72.5
Ammonium Nitrate 1650
Cupric Sulfate5H2O 0.25
Boric Acid 31
Na2 EDTA2H2O 37.3
Calcium Chloride, Anhydrous 16.61
Ferrous Sulfate7H2O 27.85
Cobalt Chloride6H2O 0.125

Plant Tissue Culture Media


Magnesium Sulfate, Anhydrous 180.7
Cupric Sulfate5H2O 0.5
Manganese SulfateH2O 22.3
Ferric Sodium EDTA 36.7
Molybdic Acid, Disodium Salt2H2O 0.25
Magnesium Sulfate, Anhydrous 903.38
Potassium Phosphate, Monobasic, Anhydrous 170
Manganese SulfateH2O 21
Zinc Sulfate7H2O 8.6
Molybdic Acid, Disodium Salt2H2O 1.25
Approximate pH at Room Temperature 4.0 0.5
Potassium Iodide 4.15
Potassium Nitrate 1900 Available Package Sizes
Potassium Phosphate, Monobasic, Anhydrous 340 1L 10L 50L
Zinc Sulfate7H2O 43
Approximate pH at Room Temperature 4.75 0.5
Available Package Sizes
1L 10L 50L

Find Phytopathology Media on Pages 161 to 164


Oat Meal Agar
Czapex & Dox Agar & Broth
Bacterial Screening Media
Potato Dextrose Agar & Broth
Corn Meal Agar

Plant Tissue Culture Media A-Z Page 83


Product Catalogue & Laboratory Guide
L449 - Lloyd & McCown Woody Plant Basal M551 - Malmgren Modified Terrestrial Orchid
Medium with Vitamins Medium Without Sucrose
Contains the macro- and micronutrients and vitamins as Contains the macro- and micronutrients, agar, and organic
described by Lloyd & McCown (1981). components as described by Malmgren (1996). Does not
Store at 2 to 6 C contain Sucrose.
Soluble in Water Store at 2 to 6 C
Use at 2.41 grams per liter Soluble in Water (Partially), Fully Soluble in Hot/Boiling
Plant Tissue Culture Tested Water
Components (mg/L) Use at 28.84 grams per liter
Plant Tissue Culture Tested
Ammonium Nitrate 400
Components (mg/L)
Boric Acid 6.2
Calcium Phosphate, Tribasic 75
Calcium Chloride, Anhydrous 72.5
Na2EDTA2H2O 37.26
Calcium Nitrate 386
Ferrous Sulfate7H2O 27.8
Cupric Sulfate5H2O 0.25
Magnesium Sulfate, Anhydrous 97.69
Plant Tissue Culture Media

Na2EDTA2H2O 37.3
Manganese SulfateH2O 1.54
Ferrous Sulfate7H2O 27.85
Potassium Phosphate, Monobasic, Anhydrous 75
Magnesium Sulfate, Anhydrous 180.7
Activated Charcoal 1000
Manganese SulfateH2O 22.3
Agar 7000
Molybdic Acid, Disodium Salt2H2O 0.25
D-Biotin 0.05
Potassium Phosphate, Monobasic, Anhydrous 170
Casein, Enzymatic Hydrolysate 400
Potassium Sulfate, Anhydrous 990
Folic Acid 0.5
Zinc Sulfate7H2O 8.6
Glycine 2.0
Glycine 2.0
myo-Inositol 100
myo-Inositol 100
Nicotinic Acid 5.0
Nicotinic Acid 0.5
Pineapple Powder 20,000
PyridoxineHCl 0.5
PyridoxineHCl 5.0
ThiamineHCl 1.0
ThiamineHCl 10
Approximate pH at Room Temperature 4.0 0.5
Approximate pH at Room Temperature 4.25 0.5
Available Package Sizes
Available Package Sizes
1L 10L 50L
1L 10L 50L

Find Microbiology Media on Pages 151 to 159


LB Agars and Broths
Tryptic Soy Broth
YEP Media
Hanahans Broth
Peptone Waters

Page 84 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
M534 - Malmgren Modified Terrestrial Orchid M482 - Malmgren Modified Terrestrial Orchid
Medium Without Sucrose & Agar Medium Without Sucrose, Agar, & Pineapple
Contains the macro- and micronutrients, and organic Powder
components as described by Malmgren (1996). Does not Contains the macro- and micronutrients, and organic
contain Sucrose or Agar. components as described by Malmgren (1996). Does not
Store at 2 to 6 C contain Sucrose, Agar, or Pineapple Powder.
Soluble in Water (Partially) Store at 2 to 6 C
Use at 21.84 grams per liter Soluble in Water (Partially), Fully Soluble in Hot/Boiling
Plant Tissue Culture Tested Water
Components (mg/L) Use at 1.84 grams per liter
Calcium Phosphate, Tribasic 75 Plant Tissue Culture Tested
Na2EDTA2H2O 37.26 Components (mg/L)
Ferrous Sulfate7H2O 27.8 Calcium Phosphate, Tribasic 75
Magnesium Sulfate, Anhydrous 97.69 Na2EDTA2H2O 37.26
Manganese SulfateH2O 1.54 Ferrous Sulfate7H2O 27.8

Plant Tissue Culture Media


Potassium Phosphate, Monobasic, Anhydrous 75 Magnesium Sulfate, Anhydrous 97.69
Activated Charcoal 1000 Manganese SulfateH2O 1.54
D-Biotin 0.05 Potassium Phosphate, Monobasic, Anhydrous 75
Casein, Enzymatic Hydrolysate 400 Activated Charcoal 1000
Folic Acid 0.5 D-Biotin 0.05
Glycine 2.0 Casein, Enzymatic Hydrolysate 400
myo-Inositol 100 Folic Acid 0.5
Nicotinic Acid 5.0 Glycine 2.0
Pineapple Powder 20,000 myo-Inositol 100
PyridoxineHCl 5.0 Nicotinic Acid 5.0
ThiamineHCl 10 PyridoxineHCl 5.0
Approximate pH at Room Temperature 4.25 0.5 ThiamineHCl 10
Approximate pH at Room Temperature 5.75 0.5
Available Package Sizes
1L 10L 50L Available Package Sizes
1L 10L 50L

Malt Extract Broth & Agar


See Microbiology Section for Complete Listings

M5516 - mD5A Medium


See Seed Testing Section for Complete Listing

Plant Tissue Culture Media A-Z Page 85


Product Catalogue & Laboratory Guide
M419 - MG Basal Salt Mixture M579 - Mitra Maintenance/Replate Medium
Modified Murashige & Skoog/Gamborg Basal Salt Mixture Contains the macro- and micronutrients, vitamins, and
Contains the macronutrients as described by Murashige & supplements as described by Mitra et al. (1976) for the culture
Skoog (1962) and the micronutrients as described by Gamborg of orchids. Contains Activated Charcoal, Agar, and Sucrose.
et al. (1968). Store at 2 to 6 C
Store at 2 to 6 C Soluble in Water (Partially), Fully Soluble in Hot/Boiling
Soluble in Water Water
Use at 1.88 grams per liter Use at 29.82 grams per liter
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Sulfate 33.5 Ammonium Sulfate 100
Boric Acid 2.3 Boric Acid 0.6
Calcium Chloride, Anhydrous 111.36 Calcium Nitrate 100
Cobalt Chloride6H2O 0.0125 Cobalt Nitrate6H2O 0.05
Cupric Sulfate5H2O 0.0125 Cupric Sulfate5H2O 0.05
Plant Tissue Culture Media

Na2 EDTA2H2O 18.64 Na2EDTA2H2O 22.3


Ferrous Sulfate7H2O 13.9 Ferrous Sulfate7H2O 16.7
Magnesium Sulfate, Anhydrous 75.7 Manganese Chloride4H2O 0.4
Manganese SulfateH2O 6.7 Magnesium Sulfate, Anhydrous 250
Molybdic Acid, Disodium Salt2H2O 0.125 Molybdic Acid, Disodium Salt2H2O 0.05
Potassium Iodide 0.4 Potassium Iodide 0.03
Potassium Nitrate 1100 Potassium Nitrate 180
Potassium Phosphate, Monobasic, Anhydrous 42.5 Sodium PhosphateH2O 150
Sodium Nitrate 437.8 Zinc Sulfate7H2O 0.05
Sodium Phosphate, MonobasicH2O 32.6 Activated Charcoal 2000
Zinc Sulfate7H2O 2.7 Agar 7000
Approximate pH at Room Temperature 4.25 0.5
D-Biotin 0.05
Available Package Sizes Folic Acid 0.3
1L 10L 50L Nicotinic Acid 1.25
PyridoxineHCl 0.3
Riboflavin 0.05
Sucrose 20,000
ThiamineHCl 0.3
Approximate pH at Room Temperature 5.75 0.75
Available Package Sizes
1L 10L 50L

Terrestrial Orchid Media Selection Guide


See "Figure 22. Terrestrial Orchid Media Selection Guide" on
page 250 in the Technical Section.

Page 86 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
M587 - Morel & Martin Vitamin Solution (100x) M5800 - Murashige & Skoog Basal Medium
Contains the vitamins as described by Morel & Martin (1955). with EDTA Ferric-Sodium Salt
myo-Inositol may precipitate out of the solution when Contains the macro- and micronutrients and vitamins as
cold; warming with occasional agitation will redissolve the described by Murashige and Skoog (1962).
precipitated myo-Inositol.
Store at 2 to 6 C
Store at 2 to 6 C Soluble in Water
Sterile Filtered Use at 4.41 grams per liter of medium
Miscible with Water Plant Tissue Culture Tested
Use at 10 mL per liter of medium
Components (mg/L)
Plant Tissue Culture Tested
Ammonium Nitrate 1650
Components (mg/L)
Boric Acid 6.2
D-Biotin 0.1
Calcium Chloride, Anhydrous 332.2
D-Calcium pantothenate 100
Cobalt Chloride6H2O 0.025
myo-Inositol 10,000
Cupric Sulfate5H2O 0.025
Nicotinic Acid 100

Plant Tissue Culture Media


FeNaEDTA 36.7
PyridoxineHCl 100
Magnesium Sulfate, Anhydrous 180.7
Approximate pH at Room Temperature 4.3 0.75
Manganese SulfateH2O 16.9
Available Package Sizes
Molybdic Acid, Disodium Salt2H2O 0.25
100mL
Potassium Iodide 0.83
M580 - Modified Melin-Norkrans Medium Potassium Nitrate 1900
See Microbiology Section for Complete Listing Potassium Phosphate, Monobasic, Anhydrous 170
Zinc Sulfate7H2O 8.6
Glycine (Free Base) 2
myo-Inositol 100
Nicotinic Acid (Free Acid) 0.5
Pyridoxine HCl 0.5
Thiamine HCl 0.1
Approximate pH at Room Temperature 5.0 0.75
Find Culture Vessels in the Equipment Section Available Package Sizes
For our full line of
1L 10L 50L 100L
culture vessels, consult
the equipment section
on pages 187 to 218.

Sterile Culture Vessels

Reusable Culture
Vessels

Petri Dishes

Culture Tubes

Plant Tissue Culture Media A-Z Page 87


Product Catalogue & Laboratory Guide
M519 - Murashige & Skoog Basal Medium with M5501 - Murashige & Skoog Basal Medium
Vitamins with Vitamins and Sucrose
Contains the macro- and micronutrients and vitamins as Contains the macro- and micronutrients and vitamins as
described by Murashige & Skoog (1962). described by Murashige & Skoog (1962).
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water Soluble in Water
Use at 4.43 grams per liter Use at 34.43 grams per liter of medium
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Nitrate 1650 Ammonium Nitrate 1650
Boric Acid 6.2 Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2 Calcium Chloride, Anhydrous 332.2
Cobalt Chloride6H2O 0.025 Cobalt Chloride6H2O 0.025
Cupric Sulfate5H2O 0.025 Cupric Sulfate5H2O 0.025
Plant Tissue Culture Media

Na2EDTA2H2O 37.26 Na2EDTA2H2O 37.26


Ferrous Sulfate7H2O 27.8 Ferrous Sulfate7H2O 27.8
Magnesium Sulfate, Anhydrous 180.7 Magnesium Sulfate, Anhydrous 180.7
Manganese SulfateH2O 16.9 Manganese SulfateH2O 16.9
Molybdic Acid, Disodium Salt 2H2O 0.25 Molybdic Acid, Disodium Salt 2H2O 0.25
Potassium Iodide 0.83 Potassium Iodide 0.83
Potassium Nitrate 1900 Potassium Nitrate 1900
Potassium Phosphate, Monobasic, Anhydrous 170 Potassium Phosphate, Monobasic, Anhydrous 170
Zinc Sulfate7H2O 8.6 Zinc Sulfate7H2O 8.6
Glycine 2.0 Glycine 2
myo-Inositol 100 myo-Inositol 100
Nicotinic Acid 0.5 Nicotinic Acid 0.5
PyridoxineHCl 0.5 PyridoxineHCl 0.5
ThiamineHCl 0.1 Sucrose 30,000
Approximate pH at Room Temperature 4.0 0.5 ThiamineHCl 0.1
Available Package Sizes Approximate pH at Room Temperature 4.0 0.5
1L 5L 10L 10LFB 50L 100L Available Package Sizes
For Find Media for the Various Stages of 1L 10L 50L 100L
Tissue Culture Plant Growth
See "Figure 5. Examples of Media Designed for a Specific Stage
of Growth" on page 231 in the Technical Section.

Page 88 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
M5530 - Murashige & Skoog Basal Medium with M5615 - Murashige & Skoog Basal Medium with
Vitamins and Sucrose Solution (1x) Vitamins, Glucose and Sucrose Solution (1x)
Contains the macro- and micronutrients and vitamins as Contains the macro- and micronutrients and vitamins as
described by Murashige & Skoog (1962). described by Murashige & Skoog (1962).
Store at 2 to 6 C Store at 2 to 6 C
Sterile Filtered Sterile Filtered
1x Solution 1x Solution
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Nitrate 1650 Ammonium Nitrate 1650
Boric Acid 6.2 Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2 Calcium Chloride, Anhydrous 332.2
Cobalt Chloride6H2O 0.025 Cobalt Chloride6H2O 0.025
Cupric Sulfate5H2O 0.025 Cupric Sulfate5H2O 0.025

Plant Tissue Culture Media


Na2EDTA2H2O 37.26 Na2EDTA2H2O 37.26
Ferrous Sulfate7H2O 27.8 Ferrous Sulfate7H2O 27.8
Magnesium Sulfate, Anhydrous 180.7 Magnesium Sulfate, Anhydrous 180.7
Manganese SulfateH2O 16.9 Manganese SulfateH2O 16.9
Molybdic Acid, Disodium Salt 2H2O 0.25 Molybdic Acid, Disodium Salt 2H2O 0.25
Potassium Iodide 0.83 Potassium Iodide 0.83
Potassium Nitrate 1900 Potassium Nitrate 1900
Potassium Phosphate, Monobasic, Anhydrous 170 Potassium Phosphate, Monobasic, Anhydrous 170
Zinc Sulfate7H2O 8.6 Zinc Sulfate7H2O 8.6
Glycine 2 Glycine 2
myo-Inositol 100 myo-Inositol 100
Nicotinic Acid 0.5 Nicotinic Acid 0.5
PyridoxineHCl 0.5 PyridoxineHCl 0.5
Sucrose 30,000 Sucrose 15,000
ThiamineHCl 0.1 Glucose 15,000
Approximate pH at Room Temperature 4.0 0.5 ThiamineHCl 0.1
Available Package Sizes Approximate pH at Room Temperature 4.0 0.5
500mL 1L Available Package Sizes
500mL 1L

Find Biochemicals on Pages 15 to 56


Gelling Agents
Plant Growth Regulators
Media Components
Stains and Dyes
Carbohydrates

Plant Tissue Culture Media A-Z Page 89


Product Catalogue & Laboratory Guide
M5707 - Murashige & Skoog Basal Medium M5825 - Murashige & Skoog Basal Medium
with Vitamins, Glucose, and Sucrose with Vitamins, Sucrose and Gelzan
Contains the macro- and micronutrients and vitamins as Contains the macro- and micronutrients and vitamins as
described by Murashige & Skoog (1962). described by Murashige & Skoog (1962).
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water Partially soluble in Water
Use at 34.43 grams per liter of medium Use at 36.43 grams per liter of medium
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Nitrate 1650 Ammonium Nitrate 1650
Boric Acid 6.2 Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2 Calcium Chloride, Anhydrous 332.2
Cobalt Chloride6H2O 0.025 Cobalt Chloride6H2O 0.025
Cupric Sulfate5H2O 0.025 Cupric Sulfate5H2O 0.025
Plant Tissue Culture Media

Na2EDTA2H2O 37.26 Na2EDTA2H2O 37.26


Ferrous Sulfate7H2O 27.8 Ferrous Sulfate7H2O 27.8
Magnesium Sulfate, Anhydrous 180.7 Magnesium Sulfate, Anhydrous 180.7
Manganese SulfateH2O 16.9 Manganese SulfateH2O 16.9
Molybdic Acid, Disodium Salt 2H2O 0.25 Molybdic Acid, Disodium Salt 2H2O 0.25
Potassium Iodide 0.83 Gelzan 2000
Potassium Nitrate 1900 Potassium Iodide 0.83
Potassium Phosphate, Monobasic, Anhydrous 170 Potassium Nitrate 1900
Zinc Sulfate7H2O 8.6 Potassium Phosphate, Monobasic, Anhydrous 170
D-Glucose 20,000 Zinc Sulfate7H2O 8.6
Glycine 2 Glycine 2
myo-Inositol 100 myo-Inositol 100
Nicotinic Acid 0.5 Nicotinic Acid 0.5
PyridoxineHCl 0.5 PyridoxineHCl 0.5
Sucrose 10,000 Sucrose 30,000
ThiamineHCl 0.1 ThiamineHCl 0.1
Approximate pH at Room Temperature 4.0 0.5 Approximate pH at Room Temperature 4.5 0.5
Available Package Sizes Available Package Sizes
1L 10L 50L 100L 1L 5L 10L 50L

Find Molecular Biology Products on Pages 131 to 150


Antibiotics
Buffers
DNA Extraction Kits
Sterile Solutions
IPTG, X-Gal & X-Gluc

Page 90 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
M5642 - Murashige & Skoog Basal Medium, M5541 - Murashige & Skoog Basal Salt Mixture
Van Der Salm Modification Van der Salm Modification
Contains the macro- and micronutrients as described by Contains the macro- and micronutrients and vitamins as
Murashige & Skoog (1962) with the iron source as described by described by Murashige & Skoog (1962) with the iron source as
Van der Salm et al. (1994). described by Van der Salm et al. (1994).
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water Soluble in Water
Use at 4.46 grams per liter Use at 4.36 grams per liter of medium
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Nitrate 1650 Ammonium Nitrate 1650
Boric Acid 6.2 Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2 Calcium Chloride, Anhydrous 332.2
Cobalt Chloride6H2O 0.025 Cobalt Chloride6H2O 0.025
Cupric Sulfate5H2O 0.025 Cupric Sulfate5H2O 0.025

Plant Tissue Culture Media


FeNa-EDDHA 96 FeNa-EDDHA 96.0
Magnesium Sulfate, Anhydrous 180.7 Magnesium Sulfate, Anhydrous 180.7
Manganese SulfateH2O 16.9 Manganese SulfateH2O 16.9
Molybdic Acid, Disodium Salt2H2O 0.25 Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Iodide 0.83 Potassium Iodide 0.83
Potassium Nitrate 1900 Potassium Nitrate 1900
Potassium Phosphate, Monobasic, Anhydrous 170 Potassium Phosphate, Monobasic, Anhydrous 170
Zinc Sulfate7H2O 8.6 Zinc Sulfate7H2O 8.6
Glycine 2 Approximate pH at Room Temperature approx. 5.0
myo-Inositol 100
Available Package Sizes
Nicotinic Acid 0.5
1L 10L 50L
PyridoxineHCl 0.5
ThiamineHCl 0.1
Approximate pH at Room Temperature 5.0 0.5
Available Package Sizes
1L 10L 50L

Plant Tissue Culture Media A-Z Page 91


Product Catalogue & Laboratory Guide
M576 - Murashige & Skoog Basal Salt M524 - Murashige & Skoog Basal Salt Mixture
Concentrate (20x) Contains the macro- and micronutrients as described by
Contains the macro- and micronutrients as described by Murashige & Skoog (1962).
Murashige & Skoog (1962). Store at 2 to 6 C
Store at 2 to 6 C Soluble in Water
Sterile Filtered Use at 4.33 grams per liter
Miscible with Water Plant Tissue Culture Tested
Use at 50.0 mL per liter Components (mg/L)
Plant Tissue Culture Tested Ammonium Nitrate 1650
Components (mg/L) Boric Acid 6.2
Ammonium Nitrate 33,000 Calcium Chloride, Anhydrous 332.2
Boric Acid 124 Cobalt Chloride6H2O 0.025
Calcium Chloride, Anhydrous 6644 Cupric Sulfate5H2O 0.025
Cobalt Chloride6H2O 0.5 Na2EDTA2H2O 37.26
Cupric Sulfate5H2O 0.5
Plant Tissue Culture Media

Ferrous Sulfate7H2O 27.8


Na2EDTA2H2O 745.2 Magnesium Sulfate, Anhydrous 180.7
Ferrous Sulfate7H2O 556 Manganese SulfateH2O 16.9
Magnesium Sulfate, Anhydrous 3614 Molybdic Acid, Disodium Salt2H2O 0.25
Manganese SulfateH2O 338 Potassium Iodide 0.83
Molybdic Acid, Disodium Salt2H2O 5.0 Potassium Nitrate 1900
Potassium Iodide 16.6 Potassium Phosphate, Monobasic, Anhydrous 170
Potassium Nitrate 38,000 Zinc Sulfate7H2O 8.6
Potassium Phosphate, Monobasic, Anhydrous 3400 Approximate pH at Room Temperature 4.0 0.5
Zinc Sulfate7H2O 172
Available Package Sizes
Approximate pH at Room Temperature 3.25 0.5
1L 5L 10L 10LFB 50L 100L
Available Package Sizes
500mL 1L For Help Preparing Tissue Culture Media
See "Figure 7. Basic Steps to Preparing 1 Liter of Medium" on
page 233 in the Technical Section.

M502 - Murashige & Skoog Macronutrient Salt


Base
Contains the macronutrients as described by Murashige &
Skoog (1962).
Store at 2 to 6 C
Soluble in Water
Use at 4.23 grams per liter
Plant Tissue Culture Tested
Components (mg/L)
Ammonium Nitrate 1650
Calcium Chloride, Anhydrous 332.2
Magnesium Sulfate, Anhydrous 180.7
Potassium Nitrate 1900
Potassium Phosphate, Monobasic, Anhydrous 170
Approximate pH at Room Temperature 4.75 0.75
Available Package Sizes
1L 10L 50L 100L

Page 92 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
M654 - Murashige & Skoog Macronutrient M5531 - Murashige & Skoog Medium with 1g/L
Stock Solution (10x) MES
Contains the macronutrients as described by Murashige & Contains the macro- and micronutrients and vitamins as
Skoog (1962). described by Murashige & Skoog (1962).
Store at 2 to 6 C Store at 2 to 6 C
Sterile Filtered Soluble in Water
Miscible with Water Use at 5.43 grams per liter
Use at 100.0 mL per liter Plant Tissue Culture Tested
Plant Tissue Culture Tested Components (mg/L)
Components (mg/L) Ammonium Nitrate 1650
Ammonium Nitrate 16,500 Boric Acid 6.2
Calcium Chloride, Anhydrous 3322 Calcium Chloride, Anhydrous 332.2
Magnesium Sulfate, Anhydrous 1807 Cobalt Chloride6H2O 0.025
Potassium Nitrate 19,000 Cupric Sulfate5H2O 0.025
Potassium Phosphate, Monobasic, Anhydrous 1700

Plant Tissue Culture Media


Na2EDTA2H2O 37.26
Approximate pH at Room Temperature 4.25 0.5 Ferrous Sulfate7H2O 27.8
Available Package Sizes Magnesium Sulfate, Anhydrous 180.7
500mL 1L Manganese SulfateH2O 16.9
Molybdic Acid, Disodium Salt 2H2O 0.25
M554 - Murashige & Skoog Micronutrient Salt
Potassium Iodide 0.83
Base
Potassium Nitrate 1900
Contains the micronutrients as described by Murashige &
Skoog (1962). Potassium Phosphate, Monobasic, Anhydrous 170
Store at -20 to 0 C Zinc Sulfate7H2O 8.6
Soluble in Water Glycine 2.0
Use at 0.10 grams per liter myo-Inositol 100
Plant Tissue Culture Tested Nicotinic Acid 0.5
Components (mg/L) PyridoxineHCl 0.5
Boric Acid 6.2 ThiamineHCl 0.1
Cobalt Chloride6H2O 0.025 MES 1000
Cupric Sulfate5H2O 0.025 Approximate pH at Room Temperature 4.0 0.5
Na2EDTA2H2O 37.26
Available Package Sizes
Ferrous Sulfate7H2O 27.8
1L 10L 50L
Manganese SulfateH2O 16.9
Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Iodide 0.83
Zinc Sulfate7H2O 8.6
Approximate pH at Room Temperature 4.3 0.5
Available Package Sizes
1L 10L 50L 100L

Plant Tissue Culture Media A-Z Page 93


Product Catalogue & Laboratory Guide
M535 - Murashige & Skoog Modified Basal M701 - Murashige & Skoog Modified Basal
Medium Medium
Contains the macro- and micronutrients as described by This medium may be sold as Murashige Begonia Multiplication
Murashige & Skoog (1962) and the vitamins described by Medium by some media suppliers. Contains the macro- and
Linsmaier & Skoog (1965). Also contains 80 mg/L Adenine micronutrients as described by Murashige & Skoog (1962)
Hemisulfate. Comporable to Linsmaier & Skoog Basal Medium and the vitamins described by Linsmaier & Skoog (1965). Also
(L689) with an added 80 mg/L Adenine Hemisulfate. contains (mg/L): 30.0 Adenine Hemisulfate, 10.0 2iP, 1.0 IAA,
Store at 2 to 6 C and Ferric Sodium EDTA in place of both Ferrous Sulfate and
Soluble in Water Disodium EDTA.
Use at 4.51 grams per liter Store at 2 to 6 C
Plant Tissue Culture Tested Soluble in Water
Components (mg/L) Use at 4.44 grams per liter
Plant Tissue Culture Tested
Ammonium Nitrate 1650
Components (mg/L)
Boric Acid 6.2
Ammonium Nitrate 1650
Calcium Chloride, Anhydrous 332.2
Plant Tissue Culture Media

Boric Acid 6.2


Cobalt Chloride6H2O 0.025
Calcium Chloride, Anhydrous 333
Cupric Sulfate5H2O 0.025
Cobalt Chloride6H2O 0.025
Na2EDTA2H2O 37.26
Cupric Sulfate5H2O 0.025
Ferrous Sulfate7H2O 27.8
Ferric Sodium EDTA 36.7
Magnesium Sulfate, Anhydrous 180.7
Magnesium Sulfate, Anhydrous 181
Manganese SulfateH2O 16.9
Manganese SulfateH2O 16.9
Molybdic Acid, Disodium Salt2H2O 0.25
Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Iodide 0.83
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Nitrate 1900
Potassium Phosphate, Monobasic, Anhydrous 170
Potassium Phosphate, Monobasic, Anhydrous 170
Zinc Sulfate7H2O 8.6
Zinc Sulfate7H2O 8.6
Adenine Hemisulfate2H2O 80
Adenine Hemisulfate2H2O 30.0
myo-Inositol 100
6-(,-Dimethylallylamino)purine; 2iP 10.0
ThiamineHCl 0.4
Indole-3-acetic Acid 1.0
Approximate pH at Room Temperature 3.75 0.5
myo-Inositol 100
Available Package Sizes
ThiamineHCl 0.4
1L 10L 50L 100L
Approximate pH at Room Temperature 4.25 0.5
Available Package Sizes
1L 10L 50L

Page 94 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
M541 - Murashige & Skoog Modified Basal M401 - Murashige & Skoog Modified Basal
Medium Medium
Contains the macro- and micronutrients as described by Contains the macro- and micronutrients and vitamins as
Murashige & Skoog (1962) and modified vitamins with the described by Murashige & Skoog (1962); modified with the
following exception: Without Potassium Phosphate Monobasic. addition of (mg/L): 1.0 BA and 0.1 NAA. Also contains 30 g/L
Also contains (mg/L): Sucrose.
300 Sodium Phosphate Monobasic, 150 Adenine Hemisulfate, Store at 2 to 6 C
1000 Casein, and Ferric Sodium EDTA in place of Ferrous Soluble in Water
Sulfate and Disodium EDTA. Use at 34.44 grams per liter
Store at 2 to 6 C Plant Tissue Culture Tested
Soluble in Water Components (mg/L)
Use at 5.69 grams per liter
Ammonium Nitrate 1650
Plant Tissue Culture Tested
Boric Acid 6.2
Components (mg/L)
Calcium Chloride, Anhydrous 332.2
Ammonium Nitrate 1650
Cobalt Chloride6H2O 0.025

Plant Tissue Culture Media


Boric Acid 6.2
Cupric Sulfate5H2O 0.025
Calcium Chloride, Anhydrous 332.2
Na2EDTA2H2O 37.26
Cobalt Chloride6H2O 0.025
Ferrous Sulfate7H2O 27.8
Cupric Sulfate5H2O 0.025
Magnesium Sulfate, Anhydrous 180.7
Ferric Sodium EDTA 36.7
Manganese SulfateH2O 16.9
Magnesium Sulfate, Anhydrous 180.7
Molybdic Acid, Disodium Salt2H2O 0.25
Manganese SulfateH2O 16.9
Potassium Iodide 0.83
Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Nitrate 1900
Potassium Iodide 0.83
Potassium Phosphate, Monobasic, Anhydrous 170
Potassium Nitrate 1900
Zinc Sulfate7H2O 8.6
Sodium Phosphate, MonobasicH2O 300
6-Benzylaminopurine 1.0
Zinc Sulfate7H2O 8.6
Glycine 2.0
Adenine Hemisulfate2H2O 150
myo-Inositol 100
Casein, Enzymatic Hydrolysate 1000
-Naphthaleneacetic Acid 0.1
Glycine 2.0
Nicotinic Acid 0.5
myo-Inositol 100
PyridoxineHCl 0.5
Nicotinic Acid 5.0
Sucrose 30,000
PyridoxineHCl 1.0
ThiamineHCl 0.4
ThiamineHCl 0.5
Approximate pH at Room Temperature 4.0 0.5
Approximate pH at Room Temperature 4.75 0.5
Available Package Sizes
Available Package Sizes
1L 10L 50L
1L 10L 50L

Plant Tissue Culture Media A-Z Page 95


Product Catalogue & Laboratory Guide
M404 - Murashige & Skoog Modified Basal M504 - Murashige & Skoog Modified Basal Salt
Medium with Gamborg Vitamins Mixture
Contains the macro- and micronutrients as described by Finer & Nagasawa (1988) Modification
Murashige & Skoog (1962) and the vitamins described by Contains the macro- and micronutrients as described by
Gamborg et al. (1968). Murashige & Skoog (1962) with the following exceptions:
Store at 2 to 6 C Contains 1.6x Potassium Nitrate, 0.5x Ammonium Nitrate,
Soluble in Water and Ferric Sodium EDTA in place of Ferrous Sulfate and
Use at 4.44 grams per liter Disodium EDTA.
Plant Tissue Culture Tested Store at 2 to 6 C
Components (mg/L) Soluble in Water
Use at 4.61 grams per liter
Ammonium Nitrate 1650
Plant Tissue Culture Tested
Boric Acid 6.2
Components (mg/L)
Calcium Chloride, Anhydrous 332.2
Ammonium Nitrate 825
Cobalt Chloride6H2O 0.025
Boric Acid 6.2
Cupric Sulfate5H2O 0.025
Plant Tissue Culture Media

Calcium Chloride, Anhydrous 332.2


Na2EDTA2H2O 37.26
Cobalt Chloride6H2O 0.025
Ferrous Sulfate7H2O 27.8
Cupric Sulfate5H2O 0.025
Magnesium Sulfate, Anhydrous 180.7
Ferric Sodium EDTA 36.7
Manganese SulfateH2O 16.9
Magnesium Sulfate, Anhydrous 180.54
Molybdic Acid, Disodium Salt2H2O 0.25
Manganese SulfateH2O 16.9
Potassium Iodide 0.83
Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Nitrate 1900
Potassium Iodide 0.83
Potassium Phosphate, Monobasic, Anhydrous 170
Potassium Nitrate 3030
Zinc Sulfate7H2O 8.6
Potassium Phosphate, Monobasic, Anhydrous 170
myo-Inositol 100
Zinc Sulfate7H2O 8.6
Nicotinic Acid 1
Approximate pH at Room Temperature 5.0 0.5
PyridoxineHCl 1
ThiamineHCl 10
Available Package Sizes
1L 10L 50L
Approximate pH at Room Temperature 4.0 0.5
Available Package Sizes
1L 10L 10LFB 50L 100L

Page 96 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
M499 - Murashige & Skoog Modified Basal Salt M290 - Murashige & Skoog Modified Basal Salt
Mixture Mixture (x Nitrates & x Calcium)
Contains the macro- and micronutrients as described by Contains the macro- and micronutrients as described by
Murashige & Skoog (1962) with the following exception: Murashige & Skoog (1962) with the following exceptions:
Contains Ferric Sodium EDTA in place of both Ferrous Sulfate Ammonium Nitrate, Potassium Nitrate, and
and Disodium EDTA. Calcium Chloride.
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water Soluble in Water
Use at 4.30 grams per liter Use at 2.39 grams per liter
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Nitrate 1650 Ammonium Nitrate 825
Boric Acid 6.2 Boric Acid 6.2
Calcium Chloride, Anhydrous 333 Calcium Chloride, Anhydrous 166.1
Cobalt Chloride6H2O 0.025 Cobalt Chloride6H2O 0.025

Plant Tissue Culture Media


Cupric Sulfate5H2O 0.025 Cupric Sulfate5H2O 0.025
Ferric Sodium EDTA 36.7 Na2EDTA2H2O 37.26
Magnesium Sulfate, Anhydrous 181 Ferrous Sulfate7H2O 27.8
Manganese SulfateH2O 16.9 Magnesium Sulfate, Anhydrous 180.7
Molybdic Acid, Disodium Salt2H2O 0.25 Manganese SulfateH2O 16.9
Potassium Iodide 0.83 Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Nitrate 1900 Potassium Iodide 0.83
Potassium Phosphate, Monobasic, Anhydrous 170 Potassium Nitrate 950
Zinc Sulfate7H2O 8.6 Potassium Phosphate, Monobasic, Anhydrous 170
Approximate pH at Room Temperature 4.75 0.5 Zinc Sulfate7H2O 8.6
Available Package Sizes Approximate pH at Room Temperature 4.0 0.5
1L 10L 50L 100L Available Package Sizes
1L 10L 50L 100L

Find Seed Testing Products on Pages 165 to 172


PhytoSelect Basal Medium
mD5A Medium
Stains & Dyes
Kings B Medium
Selection Agents

Plant Tissue Culture Media A-Z Page 97


Product Catalogue & Laboratory Guide
M153 - Murashige & Skoog Modified Basal Salt M561 - Murashige & Skoog Modified Basal Salt
Mixture (x Macros & Micros) Mixture (x Nitrates)
Contains the macro- and micronutrients as described by Contains the macro- and micronutrients as described by
Murashige & Skoog (1962). Murashige & Skoog (1962) with the following exceptions:
Store at 2 to 6 C Ammonium Nitrate and Potassium Nitrate.
Soluble in Water Store at 2 to 6 C
Use at 2.17 grams per liter Soluble in Water
Plant Tissue Culture Tested Use at 2.56 grams per liter
Components (mg/L) Plant Tissue Culture Tested
Ammonium Nitrate 825 Components (mg/L)
Boric Acid 3.1 Ammonium Nitrate 825
Calcium Chloride, Anhydrous 166.1 Boric Acid 6.2
Cobalt Chloride6H2O 0.0125 Calcium Chloride, Anhydrous 332.2
Cupric Sulfate5H2O 0.0125 Cobalt Chloride6H2O 0.025
Cupric Sulfate5H2O 0.025
Plant Tissue Culture Media

Na2EDTA2H2O 18.63
Ferrous Sulfate7H2O 13.9 Na2EDTA2H2O 37.26
Magnesium Sulfate, Anhydrous 90.35 Ferrous Sulfate7H2O 27.8
Manganese SulfateH2O 8.45 Magnesium Sulfate, Anhydrous 180.7
Molybdic Acid, Disodium Salt2H2O 0.125 Manganese SulfateH2O 16.9
Potassium Iodide 0.415 Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Nitrate 950 Potassium Iodide 0.83
Potassium Phosphate, Monobasic, Anhydrous 85 Potassium Nitrate 950
Zinc Sulfate7H2O 4.3 Potassium Phosphate, Monobasic, Anhydrous 170
Approximate pH at Room Temperature 4.25 0.5 Zinc Sulfate7H2O 8.6
Approximate pH at Room Temperature 4.0 0.5
Available Package Sizes
1L 10L 50L Available Package Sizes
1L 10L 50L

Page 98 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
M531 - Murashige & Skoog Modified Basal Salt M407 - Murashige & Skoog Modified Basal Salt
Mixture (Without Nitrogen) Mixture (Without Nitrogen, Phosphorous, and
Contains the macro- and micronutrients as described by Potassium)
Murashige & Skoog (1962) with the following exceptions: Contains the macro- and micronutrients as described by
Without Ammonium Nitrate & Potassium Nitrate. Murashige & Skoog (1962) with the following exceptions:
Store at 2 to 6 C Without Ammonium Nitrate, Potassium Nitrate, and
Soluble in Water Potassium Phosphate, Monobasic.
Use at 0.78 grams per liter Store at 2 to 6 C
Plant Tissue Culture Tested Soluble in Water
Components (mg/L) Use at 0.61 grams per liter
Boric Acid 6.2 Plant Tissue Culture Tested
Calcium Chloride, Anhydrous 332.2 Components (mg/L)
Cobalt Chloride6H2O 0.025 Boric Acid 6.2
Cupric Sulfate5H2O 0.025 Calcium Chloride, Anhydrous 332.2
Na2EDTA2H2O 37.26 Cobalt Chloride6H2O 0.025

Plant Tissue Culture Media


Ferrous Sulfate7H2O 27.8 Cupric Sulfate5H2O 0.025
Magnesium Sulfate, Anhydrous 180.7 Na2EDTA2H2O 37.26
Manganese SulfateH2O 16.9 Ferrous Sulfate7H2O 27.8
Molybdic Acid, Disodium Salt2H2O 0.25 Magnesium Sulfate, Anhydrous 180.7
Potassium Iodide 0.83 Manganese SulfateH2O 16.9
Potassium Phosphate, Monobasic, Anhydrous 170 Molybdic Acid, Disodium Salt2H2O 0.25
Zinc Sulfate7H2O 8.6 Potassium Iodide 0.83
Approximate pH at Room Temperature 4.0 0.5 Zinc Sulfate7H2O 8.6
Approximate pH at Room Temperature 4.3 0.5
Available Package Sizes
1L 10L 50L Available Package Sizes
1L 10L 50L

Tissue Culture Kits


PhytoTechnology Laboratories
offers a selection of plant tissue
culture kits for educational
use. For more information
please see pages 179 to 185.

Kits for:
African Violet,
Carrot,
Carnivorous Plants,
Fern,
Hosta,
Lily,
Orchids, and
Potato.

Plant Tissue Culture Media A-Z Page 99


Product Catalogue & Laboratory Guide
M571 - Murashige & Skoog Modified Basal Salt M516 - Murashige & Skoog Modified BC Potato
Mixture (Without NH4NO3) Basal Medium
Contains the macro- and micronutrients as described by Contains the macro- and micronutrients as described by
Murashige & Skoog (1962) with the following exception: Murashige & Skoog (1962). Contains Ferric Sodium EDTA
Without Ammonium Nitrate. in place of both Ferrous Sulfate and Disodium EDTA. Also
contains Kinetin (Product Number K750) and the vitamins
Store at 2 to 6 C cited below.
Soluble in Water
Use at 2.68 grams per liter Store at 2 to 6 C
Plant Tissue Culture Tested Soluble in Water
Use at 4.41 grams per liter
Components (mg/L) Plant Tissue Culture Tested
Boric Acid 6.2
Components (mg/L)
Calcium Chloride, Anhydrous 332.2
Ammonium Nitrate 1650
Cobalt Chloride6H2O 0.025
Boric Acid 6.2
Cupric Sulfate5H2O 0.025
Calcium Chloride, Anhydrous 333
Plant Tissue Culture Media

Na2EDTA2H2O 37.26
Cobalt Chloride6H2O 0.025
Ferrous Sulfate7H2O 27.8
Cupric Sulfate5H2O 0.025
Magnesium Sulfate, Anhydrous 180.7
Ferric Sodium EDTA 36.7
Manganese SulfateH2O 16.9
Magnesium Sulfate, Anhydrous 181
Molybdic Acid, Disodium Salt2H2O 0.25
Manganese SulfateH2O 16.9
Potassium Iodide 0.83
Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Nitrate 1900
Potassium Iodide 0.83
Potassium Phosphate, Monobasic, Anhydrous 170
Potassium Nitrate 1900
Zinc Sulfate7H2O 8.6
Potassium Phosphate, Monobasic, Anhydrous 170
Approximate pH at Room Temperature 4.0 0.5
Zinc Sulfate7H2O 8.6
Available Package Sizes Glycine 2.0
1L 10L 50L myo-Inositol 100
Kinetin 0.04
Nicotinic Acid 0.5
PyridoxineHCl 0.5
ThiamineHCl 0.4
Approximate pH at Room Temperature 4.75 0.5
Available Package Sizes
1L 10L 50L

Page 100 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
M702 - Murashige & Skoog Modified Medium M550 - Murashige & Skoog Modified Medium
Contains the macro- and micronutrients as described by Arabidopsis Culture Medium
Murashige and Skoog (1962) and the vitamins described by Contains the macro- and micronutrients as described by
Linsmaier and Skoog (1965). Contains 30 g/L Sucrose. Murashige and Skoog (1962) and modified vitamins. Contains
Also contains: 148 mg/L Sodium Phosphate Monobasic, 80 20 g/L Sucrose. Also contains: 2.0 mg/L 2,4-D and 0.05 mg/L
mg/L Adenine Hemisulfate, 30 mg/L 2iP, and 0.3 mg/L IAA. Kinetin.
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water Soluble in Water
Use at 34.69 grams per liter Use at 24.44 grams per liter
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Nitrate 1650 Ammonium Nitrate 1650
Boric Acid 6.2 Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2 Calcium Chloride, Anhydrous 332.2
Cobalt Chloride6H2O 0.025 Cobalt Chloride6H2O 0.025

Plant Tissue Culture Media


Cupric Sulfate5H2O 0.025 Cupric Sulfate5H2O 0.025
Na2EDTA2H2O 37.26 Na2EDTA2H2O 37.26
Ferrous Sulfate7H2O 27.8 Ferrous Sulfate7H2O 27.8
Magnesium Sulfate, Anhydrous 180.7 Magnesium Sulfate, Anhydrous 180.7
Manganese SulfateH2O 16.9 Manganese SulfateH2O 16.9
Molybdic Acid, Disodium Salt2H2O 0.25 Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Iodide 0.83 Potassium Iodide 0.83
Potassium Nitrate 1900 Potassium Nitrate 1900
Potassium Phosphate, Monobasic, Anhydrous 170 Potassium Phosphate, Monobasic, Anhydrous 170
Sodium Phosphate, MonobasicH2O 148 Zinc Sulfate7H2O 8.6
Zinc Sulfate7H2O 8.6 2,4-Dichlorophenoxyacetic Acid 2.0
Adenine Hemisulfate2H2O 80 myo-Inositol 100
6-(,-Dimethylallylamino)purine; 2iP 30 Kinetin 0.05
Indole-3-acetic Acid 0.3 Nicotinic Acid 1.0
myo-Inositol 100 PyridoxineHCl 1.0
Sucrose 30,000 Sucrose 20,000
ThiamineHCl 0.4 ThiamineHCl 10.0
Approximate pH at Room Temperature 4.0 0.75 Approximate pH at Room Temperature 3.75 0.5
Available Package Sizes Available Package Sizes
1L 10L 50L 1L 10L 50L

Plant Tissue Culture Media A-Z Page 101


Product Catalogue & Laboratory Guide
M555 - Murashige & Skoog Modified M547 - Murashige & Skoog Modified Vitamin
Multiplication Medium Powder (1000x)
This medium may be sold as Murashige & Skoog (MS) Shoot Contains the vitamins as described by Murashige & Skoog
Multiplication Medium C by some media suppliers. (1962) modified with additional Thiamine HCl (Product
Contains the macro- and micronutrients as described by Number T390).
Murashige and Skoog (1962) and the vitamins described by myo-Inositol may precipitate out of the solution when
Linsmaier and Skoog (1965). Contains 30 g/L Sucrose. cold; warming with occasional agitation will redissolve the
Also contains: 148 mg/L Sodium Phosphate Monobasic, 80 precipitated myo-Inositol.
mg/L Adenine Hemisulfate, 1.0 mg/L Kinetin, 0.1 mg/L NAA. Store at 2 to 6 C
Store at 2 to 6 C Soluble in Water
Soluble in Water Use 10.40 grams to make 100 mL
Use at 34.66 grams per liter Use at 1.0 mL per liter of Medium
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Nitrate 1650 Glycine 2000
Plant Tissue Culture Media

Boric Acid 6.2 myo-Inositol 100,000


Calcium Chloride, Anhydrous 332.2 Nicotinic Acid 500
Cobalt Chloride6H2O 0.025 PyridoxineHCl 500
Cupric Sulfate5H2O 0.025 ThiamineHCl 1000
Na2EDTA2H2O 37.26 Approximate pH at Room Temperature 4.0 0.5
Ferrous Sulfate7H2O 27.8 Available Package Sizes
Magnesium Sulfate, Anhydrous 180.7 100mL 250mL
Manganese SulfateH2O 16.9
Molybdic Acid, Disodium Salt2H2O 0.25
Vitamins Mixtures for use in Plant Tissue Culture
See "Vitamin Formulations Table" on page 287 in the
Potassium Iodide 0.83
Technical Section.
Potassium Nitrate 1900
Potassium Phosphate, Monobasic, Anhydrous 170 M557 - Murashige & Skoog Modified Vitamin
Sodium Phosphate, MonobasicH2O 148 Solution (1000x)
Zinc Sulfate7H2O 8.6 Contains the vitamins as described by Murashige & Skoog
Adenine Hemisulfate2H2O 80 (1962) modified with additional Thiamine HCl Product
Number T390).
myo-Inositol 100
myo-Inositol may precipitate out of the solution when
Kinetin 1.0 cold; warming with occasional agitation will redissolve the
-Naphthaleneacetic Acid 0.1 precipitated myo-Inositol.
Sucrose 30,000 Store at 2 to 6 C
ThiamineHCl 0.4 Sterile Filtered
Miscible with Water
Approximate pH at Room Temperature 3.75 0.5
Use at 1.0 mL per liter of Medium
Available Package Sizes Plant Tissue Culture Tested
1L 10L 50L Components (mg/L)
Glycine 2000
myo-Inositol 100,000
Nicotinic Acid 500
PyridoxineHCl 500
ThiamineHCl 1000
Approximate pH at Room Temperature 3.75 0.5
Available Package Sizes
100 mL

Page 102 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
M553 - Murashige & Skoog Vitamin Solution M517 - Murashige Modified African Violet/
(1000x) Gloxinia Multiplication Basal Medium
Contains the vitamins as described by Murashige & Skoog Contains the macro- and micronutrients as described by
(1962). Murashige & Skoog (1962) and the vitamins described by
myo-Inositol may precipitate out of the solution when Linsmaier & Skoog (1965). Also contains:
cold; warming with occasional agitation will redissolve the 170 mg/L Sodium Phosphate Monobasic, 80 mg/L Adenine
precipitated myo-Inositol. Hemisulfate, 2.0 mg/L IAA, 2.0 mg/L Kinetin, and Ferric
Store at 2 to 6 C Sodium EDTA in place of Ferrous Sulfate and Disodium
Sterile Filtered EDTA.
Miscible with Water Store at 2 to 6 C
Use at 1.0 mL per liter of Medium Soluble in Water
Plant Tissue Culture Tested Use at 4.66 grams per liter
Components (mg/L) Plant Tissue Culture Tested
Glycine 2000 Components (mg/L)
myo-Inositol 100,000 Ammonium Nitrate 1650

Plant Tissue Culture Media


Nicotinic Acid 500 Boric Acid 6.2
PyridoxineHCl 500 Calcium Chloride, Anhydrous 333
ThiamineHCl 100 Cobalt Chloride6H2O 0.025
Approximate pH at Room Temperature 4.0 0.5 Cupric Sulfate5H2O 0.025
Ferric Sodium EDTA 36.7
Available Package Sizes
Magnesium Sulfate, Anhydrous 181
100 mL
Manganese SulfateH2O 16.9
Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic, Anhydrous 170
Sodium Phosphate, MonobasicH2O 170
Zinc Sulfate7H2O 8.6
Molecular Biology Kits Adenine Hemisulfate2H2O 80
Indole-3-acetic Acid 2.0
PhytoTechnology Laboratories offers a variety
of kits for molecular biology. Please see the Kinetin 2.0
molecular biology section on pages 131 to myo-Inositol 100
150 for more information. ThiamineHCl 0.4
Approximate pH at Room Temperature 4.0 0.5
RNA Extraction Kits
Available Package Sizes
Genomic DNA Extraction Kits
1L 10L 50L
Plasmid DNA Extraction Kits

DNA Ladder Kits

Plant Tissue Culture Media A-Z Page 103


Product Catalogue & Laboratory Guide
M518 - Murashige Modified African Violet/ M507 - Murashige Modified Cattleya Orchid
Gloxinia Pretransplant Basal Medium Multiplication Medium
Contains the macro- and micronutrients as described by Contains the macro- and micronutrients as described by
Murashige & Skoog (1962) and the vitamins described by Murashige & Skoog (1962) and modified vitamins. Contains
Linsmaier & Skoog (1965). Also contains: 1.0 mg/L IAA, and 20 g/L Sucrose. Also contains: 150 mg/L Citric Acid, 0.3 mg/L
Ferric Sodium EDTA in place of Ferrous Sulfate and Disodium IAA, 1.75 mg/L IBA, 1.75 mg/L NAA, and Ferric Sodium
EDTA. EDTA in place of Ferrous Sulfate and Disodium EDTA.
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water Soluble in Water
Use at 4.40 grams per liter Use at 24.57 grams per liter
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Nitrate 1650 Ammonium Nitrate 1650
Boric Acid 6.2 Boric Acid 6.2
Calcium Chloride, Anhydrous 333 Calcium Chloride, Anhydrous 333
Plant Tissue Culture Media

Cobalt Chloride6H2O 0.025 Cobalt Chloride6H2O 0.025


Cupric Sulfate5H2O 0.025 Cupric Sulfate5H2O 0.025
Ferric Sodium EDTA 36.7 Ferric Sodium EDTA 36.7
Magnesium Sulfate, Anhydrous 181 Magnesium Sulfate, Anhydrous 181
Manganese SulfateH2O 16.9 Manganese SulfateH2O 16.9
Molybdic Acid, Disodium Salt2H2O 0.25 Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Iodide 0.83 Potassium Iodide 0.83
Potassium Nitrate 1900 Potassium Nitrate 1900
Potassium Phosphate, Monobasic, Anhydrous 170 Potassium Phosphate, Monobasic, Anhydrous 170
Zinc Sulfate7H2O 8.6 Zinc Sulfate7H2O 8.6
Indole-3-acetic Acid 1.0 Citric Acid, Anhydrous 150
myo-Inositol 100 Glycine 2.0
ThiamineHCI 0.4 Indole-3-acetic Acid 0.3
Approximate pH at Room Temperature 4.75 0.5 Indole-3-butyric Acid 1.75
Available Package Sizes myo-Inositol 100
1L 10L 50L -Naphthaleneacetic Acid 1.75
Nicotinic Acid 0.5
PyridoxineHCl 0.5
Sucrose 20,000
ThiamineHCl 10
Approximate pH at Room Temperature 3.25 0.5
Available Package Sizes
1L 10L 50L

Selecting Epiphytic Orchid Media by Genus


See "Figure 25. Epiphytic Orchid Media Selection Guide by
Genus" on page 252 in the Technical Section.

Page 104 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
M508 - Murashige Modified Fern Multiplication M509 - Murashige Modified Gerbera
Basal Medium Multiplication Basal Medium
Contains the macro- and micronutrients as described by Contains the macro- and micronutrients as described by
Murashige & Skoog (1962) and the vitamins described by Murashige & Skoog (1962) and modified vitamins. Also
Linsmaier & Skoog (1965). Also contains: 255 mg/L Sodium contains: 85 mg/L Sodium Phosphate Monobasic, 80 mg/L
Phosphate Monobasic, 2.0 mg/L Kinetin, 0.1 mg/L NAA, and Adenine Hemisulfate, 10.0 mg/L Kinetin, 0.5 mg/L IAA, 100
Ferric Sodium EDTA in place of Ferrous Sulfate and Disodium mg/L L-Tyrosine, and Ferric Sodium EDTA in place of Ferrous
EDTA. Sulfate and Disodium EDTA.
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water Soluble in Water
Use at 4.66 grams per liter Use at 4.72 grams per liter
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Nitrate 1650 Ammonium Nitrate 1650
Boric Acid 6.2 Boric Acid 6.2

Plant Tissue Culture Media


Calcium Chloride, Anhydrous 333 Calcium Chloride, Anhydrous 333
Cobalt Chloride6H2O 0.025 Cobalt Chloride6H2O 0.025
Cupric Sulfate5H2O 0.025 Cupric Sulfate5H2O 0.025
Ferric Sodium EDTA 36.7 Ferric Sodium EDTA 36.7
Magnesium Sulfate, Anhydrous 181 Magnesium Sulfate, Anhydrous 181
Manganese SulfateH2O 16.9 Manganese SulfateH2O 16.9
Molybdic Acid, Disodium Salt2H2O 0.25 Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Iodide 0.83 Potassium Iodide 0.83
Potassium Nitrate 1900 Potassium Nitrate 1900
Potassium Phosphate, Monobasic, Anhydrous 170 Potassium Phosphate, Monobasic, Anhydrous 170
Sodium Phosphate, MonobasicH2O 255 Sodium Phosphate, MonobasicH2O 85
Zinc Sulfate7H2O 8.6 Zinc Sulfate7H2O 8.6
Kinetin 2.0 Adenine Hemisulfate2H2O 80
myo-Inositol 100 Kinetin 10.0
-Naphthaleneacetic Acid 0.1 Indole-3-acetic Acid 0.5
ThiamineHCI 0.4 myo-Inositol 100
Approximate pH at Room Temperature 4.75 0.5 Nicotinic Acid 10.0
Available Package Sizes PyridoxineHCl 1.0
1L 10L 50L ThiamineHCl 30
L-Tyrosine 100
Approximate pH at Room Temperature 4.0 0.5
Available Package Sizes
1L 10L 50L

Plant Tissue Culture Media A-Z Page 105


Product Catalogue & Laboratory Guide
M510 - Murashige Modified Gerbera M511 - Murashige Modified Kalanchoe
Pretransplant Basal Medium Multiplication Basal Medium
Contains the macro- and micronutrients as described by Contains the macro- and micronutrients as described by
Murashige & Skoog (1962) and modified vitamins. Also Murashige & Skoog (1962) and the vitamins described by
contains: 85 mg/L Sodium Phosphate Monobasic, 10.0 mg/L Linsmaier & Skoog (1965). Also contains: 3.0 mg/L 2iP and
IAA, 100 mg/L L-Tyrosine, and Ferric Sodium EDTA in place Ferric Sodium EDTA in place of Ferrous Sulfate and Disodium
of Ferrous Sulfate and Disodium EDTA. EDTA.
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water Soluble in Water
Use at 4.64 grams per liter Use at 4.41 grams per liter
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Nitrate 1650 Ammonium Nitrate 1650
Boric Acid 6.2 Boric Acid 6.2
Calcium Chloride, Anhydrous 333 Calcium Chloride, Anhydrous 333
Plant Tissue Culture Media

Cobalt Chloride6H2O 0.025 Cobalt Chloride6H2O 0.025


Cupric Sulfate5H2O 0.025 Cupric Sulfate5H2O 0.025
Ferric Sodium EDTA 36.7 Ferric Sodium EDTA 36.7
Magnesium Sulfate, Anhydrous 181.7 Magnesium Sulfate, Anhydrous 181
Manganese SulfateH2O 16.9 Manganese SulfateH2O 16.9
Molybdic Acid, Disodium Salt2H2O 0.25 Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Iodide 0.83 Potassium Iodide 0.83
Potassium Nitrate 1900 Potassium Nitrate 1900
Potassium Phosphate, Monobasic, Anhydrous 170 Potassium Phosphate, Monobasic, Anhydrous 170
Sodium Phosphate, MonobasicH2O 85 Zinc Sulfate7H2O 8.6
Zinc Sulfate7H2O 8.6 myo-Inositol 100
myo-Inositol 100 6-(,-Dimethylallylamino)purine; 2iP 3.0
Indole-3-acetic Acid 10 ThiamineHCl 0.4
Nicotinic Acid 10 Approximate pH at Room Temperature 4.75 0.5
PyridoxineHCI 1.0 Available Package Sizes
ThiamineHCI 30 1L 10L 50L
L-Tyrosine 100
Approximate pH at Room Temperature 4.25 0.5
Available Package Sizes
1L 10L 50L

Page 106 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
M512 - Murashige Modified Kalanchoe M536 - Murashige Modified Multiplication Basal
Pretransplant Basal Medium Medium
This medium may be sold as Murashige Syngonium Contains the macro- and micronutrients as described by
Pretransplant Medium by some media suppliers. Murashige & Skoog (1962) and modified vitamins. Also
Contains the macro- and micronutrients as described by contains: 170 mg/L Sodium Phosphate Monobasic and
Murashige & Skoog (1962) and the vitamins described by 80 mg/L Adenine Hemisulfate.
Linsmaier & Skoog (1965). Also contains: 3.0 mg/L IAA and Store at 2 to 6 C
Ferric Sodium EDTA in place of Ferrous Sulfate and Disodium Soluble in Water
EDTA. Use at 4.68 grams per liter
Store at 2 to 6 C Plant Tissue Culture Tested
Soluble in Water Components (mg/L)
Use at 4.41 grams per liter
Ammonium Nitrate 1650
Plant Tissue Culture Tested
Boric Acid 6.2
Components (mg/L)
Calcium Chloride, Anhydrous 332.2
Ammonium Nitrate 1650
Cobalt Chloride6H2O 0.025

Plant Tissue Culture Media


Boric Acid 6.2
Cupric Sulfate5H2O 0.025
Calcium Chloride, Anhydrous 333
Na2EDTA2H2O 37.26
Cobalt Chloride6H2O 0.025
Ferrous Sulfate7H2O 27.8
Cupric Sulfate5H2O 0.025
Magnesium Sulfate, Anhydrous 180.7
Ferric Sodium EDTA 36.7
Manganese SulfateH2O 16.9
Magnesium Sulfate, Anhydrous 181
Molybdic Acid, Disodium Salt2H2O 0.25
Manganese SulfateH2O 16.9
Potassium Iodide 0.83
Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Nitrate 1900
Potassium Iodide 0.83
Potassium Phosphate, Monobasic, Anhydrous 170
Potassium Nitrate 1900
Sodium Phosphate, MonobasicH2O 170
Potassium Phosphate, Monobasic, Anhydrous 170
Zinc Sulfate7H2O 8.6
Zinc Sulfate7H2O 8.6
Adenine Hemisulfate2H2O 80
myo-Inositol 100
Glycine 2
Indole-3-acetic Acid 3.0
myo-Inositol 100
ThiamineHCl 0.4
Nicotinic Acid 0.5
Approximate pH at Room Temperature 4.75 0.5
PyridoxineHCl 0.5
Available Package Sizes
ThiamineHCl 0.4
1L 10L 50L
Approximate pH at Room Temperature 3.75 0.5
Available Package Sizes
1L 10L 50L 100L

Plant Tissue Culture Media A-Z Page 107


Product Catalogue & Laboratory Guide
M527 - Murashige Modified Multiplication Basal M491 - Murashige Modified Shoot Multiplication
Medium Basal Medium
This medium may be sold as Murashige & Skoog (MS) Shoot This medium may be sold as Murashige & Skoog (MS) Shoot
Tip Rooting Medium by some media suppliers. Contains the Multiplication Medium A by some media suppliers. Contains
macro- and micronutrients as described by Murashige & Skoog the macro- and micronutrients as described by Murashige
(1962) and the vitamins described by Linsmaier & Skoog & Skoog (1962) and the vitamins described by Linsmaier &
(1965). Also contains: 1.0 mg/L Kinetin, 0.3 mg/L IAA, and Skoog (1965). Also contains: 170 mg/L Sodium Phosphate
Ferric Sodium EDTA in place of Ferrous Sulfate and Disodium Monobasic, 80 mg/L Adenine Hemisulfate, 30.0 mg/L 2iP,
EDTA. 0.3 mg/L IAA, and Ferric Sodium EDTA in place of Ferrous
Store at 2 to 6 C Sulfate and Disodium EDTA.
Soluble in Water Store at 2 to 6 C
Use at 4.41 grams per liter Soluble in Water
Plant Tissue Culture Tested Use at 4.68 grams per liter
Components (mg/L) Plant Tissue Culture Tested
Ammonium Nitrate 1650 Components (mg/L)
Ammonium Nitrate 1650
Plant Tissue Culture Media

Boric Acid 6.2


Calcium Chloride, Anhydrous 333 Boric Acid 6.2
Cobalt Chloride6H2O 0.025 Calcium Chloride, Anhydrous 333
Cupric Sulfate5H2O 0.025 Cobalt Chloride6H2O 0.025
Ferric Sodium EDTA 36.7 Cupric Sulfate5H2O 0.025
Magnesium Sulfate, Anhydrous 181 Ferric Sodium EDTA 36.7
Manganese SulfateH2O 16.9 Magnesium Sulfate, Anhydrous 181
Molybdic Acid, Disodium Salt2H2O 0.25 Manganese SulfateH2O 16.9
Potassium Iodide 0.83 Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Nitrate 1900 Potassium Iodide 0.83
Potassium Phosphate, Monobasic, Anhydrous 170 Potassium Nitrate 1900
Zinc Sulfate7H2O 8.6 Potassium Phosphate, Monobasic, Anhydrous 170
Indole-3-acetic Acid 0.3 Sodium Phosphate, MonobasicH2O 170
myo-Inositol 100 Zinc Sulfate7H2O 8.6
Kinetin 1.0 Adenine Hemisulfate2H2O 80
ThiamineHCl 0.4 6-(,-Dimethylallylamino)purine; 2iP 30.0
Approximate pH at Room Temperature 4.75 0.5 Indole-3-acetic Acid 0.3
myo-Inositol 100
Available Package Sizes
ThiamineHCl 0.4
1L 10L 50L
Approximate pH at Room Temperature 4.0 0.5
Available Package Sizes
1L 10L 50L

Find Phycology Products on Pages 173 to 178


Bolds Basal Medium
Blue-Green Medium
Tris Acetate Phosphate
Seawater
Sueokas High Salt Medium

Page 108 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
M462 - Musa (Banana) Multiplication Medium N492 - NB Basal Medium
IITA formulation as described by Vuylsteke (1989) Modified Chu/Gamborg Basal Medium
(International Institute for Tropical Agriculture) Contains the macronutrients as described by Chu (1975) and
Contains PGRs, sucrose, and gelling agent. the micronutrients as described by Gamborg et al. (1968).
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water (Partially) Soluble in Water
Use at 36.36 grams per liter Use at 4.10 grams per liter
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Nitrate 1650 Ammonium Sulfate 463
Boric Acid 6.2 Boric Acid 3.0
Calcium Chloride, Anhydrous 332.2 Calcium Chloride, Anhydrous 125.33
Cobalt Chloride6H2O 0.025 Cobalt Chloride6H2O 0.025
Cupric Sulfate5H2O 0.025 Cupric Sulfate5H2O 0.025
Na2EDTA2H2O 37.25 Na2 EDTA2H2O 37.26

Plant Tissue Culture Media


Ferrous Sulfate7H2O 27.85 Ferrous Sulfate7H2O 27.8
Magnesium Sulfate, Anhydrous 180.74 Magnesium Sulfate, Anhydrous 90.37
Manganese SulfateH2O 16.9 Manganese SulfateH2O 10
Molybdic Acid, Disodium Salt2H2O 0.25 Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Iodide 0.83 Potassium Iodide 0.75
Potassium Nitrate 1900 Potassium Nitrate 2830
Potassium Phosphate, Monobasic, Anhydrous 170 Potassium Phosphate, Monobasic, Anhydrous 400
Zinc Sulfate7H2O 8.6 Zinc Sulfate7H2O 2.0
L-Ascorbic Acid 20 myo-Inositol 100
6-Benzylaminopurine 4.5 Nicotinic Acid 1.0
Gellan Gum Biotech Grade 2000 PyridoxineHCI 1.0
Glycine 2.0 ThiamineHCI 10
Indole-3-acetic Acid 0.175 Approximate pH at Room Temperature 4.0 0.5
Nicotinic Acid 0.5 Available Package Sizes
PyridoxineHCl 0.5 1L 4L 10L 50L
Sucrose 30,000
ThiamineHCl 0.4
Approximate pH at Room Temperature 4.25 0.5
Available Package Sizes
1L 10L 50L

Plant Tissue Culture Media A-Z Page 109


Product Catalogue & Laboratory Guide
N616 - Nitsch & Nitsch Basal Medium with N613 - Nitsch & Nitsch Basal Salt Mixture
Vitamins Contains the macro- and micronutrients as described by Nitsch
Contains the macro- and micronutrients and vitamins as & Nitsch (1969).
described by Nitsch & Nitsch (1969). Store at 2 to 6 C
Store at 2 to 6 C Soluble in Water
Soluble in Water Use at 2.10 grams per liter
Use at 2.21 grams per liter Plant Tissue Culture Tested
Plant Tissue Culture Tested Components (mg/L)
Components (mg/L) Ammonium Nitrate 720
Ammonium Nitrate 720 Boric Acid 10
Boric Acid 10 Calcium Chloride, Anhydrous 166
Calcium Chloride, Anhydrous 166 Cupric Sulfate5H2O 0.025
Cupric Sulfate5H2O 0.025 Na2EDTA2H2O 37.26
Na2EDTA2H2O 37.26 Ferrous Sulfate7H2O 27.8
Plant Tissue Culture Media

Ferrous Sulfate7H2O 27.8 Magnesium Sulfate, Anhydrous 90.372


Magnesium Sulfate, Anhydrous 90.372 Manganese SulfateH2O 18.9
Manganese SulfateH2O 18.9 Molybdic Acid, Disodium Salt2H2O 0.25
Molybdic Acid, Disodium Salt2H2O 0.25 Potassium Nitrate 950
Potassium Nitrate 950 Potassium Phosphate, Monobasic, Anhydrous 68
Potassium Phosphate, Monobasic, Anhydrous 68 Zinc Sulfate7H2O 10
Zinc Sulfate7H2O 10 Approximate pH at Room Temperature 4.0 0.5
D-Biotin 0.05 Available Package Sizes
Folic Acid 0.5 1L 10L 50L 100L
Glycine 2.0
myo-Inositol 100 N608 - Nitsch & Nitsch Vitamin Powder (1000x)
Nicotinic Acid 5.0 Contains the vitamins as described by Nitsch & Nitsch (1969).
myo-Inositol may precipitate out of the solution when
PyridoxineHCl 0.5 cold; warming with occasional agitation will redissolve the
ThiamineHCl 0.5 precipitated myo-Inositol.
Approximate pH at Room Temperature 4.0 0.5 Store at 2 to 6 C
Available Package Sizes Soluble in Water
Use 10.86 grams to make 100 mL
1L 10L 50L
Use at 1.0 mL per liter of Medium
Plant Tissue Culture Tested
Components (mg/L)
D-Biotin 50
Folic Acid 500
Glycine 2000
myo-Inositol 100,000
Nicotinic Acid 5000
PyridoxineHCl 500
ThiamineHCl 500
Approximate pH at Room Temperature 3.75 0.75
Available Package Sizes
100mL 250mL

Page 110 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
N603 - Nitsch & Nitsch Vitamin Solution (1000x) N479 - NLN Basal Medium
Contains the vitamins as described by Nitsch & Nitsch (1969). Contains the macro- and micronutrients and organic
myo-Inositol may precipitate out of the solution when components as described by Lichter (1982).
cold; warming with occasional agitation will redissolve the Store at 2 to 6 C
precipitated myo-Inositol. Soluble in Water
Store at 2 to 6 C Use at 1.77 grams per liter
Sterile Filtered Plant Tissue Culture Tested
Miscible with Water Components (mg/L)
Use at 1.0 mL per liter of medium
Boric Acid 10
Plant Tissue Culture Tested
Calcium Nitrate 347
Components (mg/L)
Cobalt Chloride6H2O 0.025
D-Biotin 50
Cupric Sulfate5H2O 0.025
Folic Acid 500
Ferric Sodium EDTA 36.7
Glycine 2000
Magnesium Sulfate, Anhydrous 61
myo-Inositol 100,000

Plant Tissue Culture Media


Manganese SulfateH2O 18.95
Nicotinic Acid 5000
Molybdic Acid, Disodium Salt2H2O 0.25
PyridoxineHCl 500
Potassium Nitrate 125
ThiamineHCl 500
Potassium Phosphate, Monobasic, Anhydrous 125
Approximate pH at Room Temperature 8.0 0.75
Zinc Sulfate7H2O 10
Available Package Sizes
D-Biotin 0.05
100mL
Folic Acid 0.5
L-Glutamine 800
Glutathione (Reduced) 30
Glycine 2
myo-Inositol 100
Nicotinic Acid 5
PyridoxineHCl 0.5
L-Serine 100
Custom Media & Services
ThiamineHCl 0.5
PhytoTechnology Laboratories
Approximate pH at Room Temperature 4.75 0.5
offers custom manufacturing
and testing services. For more Available Package Sizes
information please see page 1L 10L 50L
12.
Nutrient Broth & Agar
Custom Dry Powder Media See Microbiology Section for Complete Listings
Custom Liquid Media
O606 - Oat Meal Agar
Custom Packaging See Phytopathology Section for Complete Listing

Testing Services

Plant Tissue Culture Media A-Z Page 111


Product Catalogue & Laboratory Guide
O139 - Orchid Maintenance/Replate Medium O156 - Orchid Maintenance/Replate Medium
Without Charcoal and Agar Contains Banana Powder (Product Number B852) and
Store at 2 to 6 C Charcoal (Product Number C325), Without Agar
Soluble in Water Store at 2 to 6 C
Use at 25.31 grams per liter Soluble in Water (Partially)
Plant Tissue Culture Tested Use at 57.31 grams per liter
Components (mg/L) Plant Tissue Culture Tested
Ammonium Nitrate 825 Components (mg/L)
Boric Acid 3.1 Ammonium Nitrate 825
Calcium Chloride, Anhydrous 166 Boric Acid 3.1
Cobalt Chloride6H2O 0.0125 Calcium Chloride, Anhydrous 166
Cupric Sulfate5H2O 0.0125 Cobalt Chloride6H2O 0.0125
Na2EDTA2H2O 37.3 Cupric Sulfate5H2O 0.0125
Ferrous Sulfate7H2O 27.85 Na2EDTA2H2O 37.3
Plant Tissue Culture Media

Magnesium Sulfate, Anhydrous 90.35 Ferrous Sulfate7H2O 27.85


Manganese SulfateH2O 8.45 Magnesium Sulfate, Anhydrous 90.35
Molybdic Acid, Disodium Salt2H2O 0.125 Manganese SulfateH2O 8.45
Potassium Iodide 0.415 Molybdic Acid, Disodium Salt2H2O 0.125
Potassium Nitrate 950 Potassium Iodide 0.415
Potassium Phosphate, Monobasic, Anhydrous 85 Potassium Nitrate 950
Zinc Sulfate7H2O 5.3 Potassium Phosphate, Monobasic, Anhydrous 85
myo-Inositol 100 Zinc Sulfate7H2O 5.3
MESH2O 1000 Activated Charcoal 2000
Nicotinic Acid 1.0 Banana Powder 30,000
Peptone from Meat 2000 myo-Inositol 100
PyridoxineHCl 1.0 MESH2O 1000
Sucrose 20,000 Nicotinic Acid 1.0
ThiamineHCl 10 Peptone from Meat 2000
Approximate pH at Room Temperature 5.25 0.5 PyridoxineHCl 1.0
Sucrose 20,000
Available Package Sizes
ThiamineHCl 10
1L 10L 50L
Approximate pH at Room Temperature 5.0 0.5
Available Package Sizes
1L 10L 50L

Page 112 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
P748 - Orchid Maintenance/Replate Medium P658 - Orchid Maintenance Medium
Contains Banana Powder (Product Number B852) and Contains Charcoal (Product Number C325) and Agar (Product
Charcoal (Product Number C325), and Agar (Product Number Number A111)
A111) Store at 2 to 6 C
Store at 2 to 6 C Soluble in Water (Partially)
Soluble in Water (Partially) Use at 35.31 grams per liter
Use at 64.31 grams per liter Plant Tissue Culture Tested
Plant Tissue Culture Tested Components (mg/L)
Components (mg/L) Ammonium Nitrate 825
Ammonium Nitrate 825 Boric Acid 3.1
Boric Acid 3.1 Calcium Chloride, Anhydrous 166
Calcium Chloride, Anhydrous 166 Cobalt Chloride6H2O 0.0125
Cobalt Chloride6H2O 0.0125 Cupric Sulfate5H2O 0.0125
Cupric Sulfate5H2O 0.0125 Na2EDTA2H2O 37.3
Na2EDTA2H2O 37.3

Plant Tissue Culture Media


Ferrous Sulfate7H2O 27.85
Ferrous Sulfate7H2O 27.85 Magnesium Sulfate, Anhydrous 90.35
Magnesium Sulfate, Anhydrous 90.35 Manganese SulfateH2O 8.45
Manganese SulfateH2O 8.45 Molybdic Acid, Disodium Salt2H2O 0.125
Molybdic Acid, Disodium Salt2H2O 0.125 Potassium Iodide 0.415
Potassium Iodide 0.415 Potassium Nitrate 950
Potassium Nitrate 950 Potassium Phosphate, Monobasic, Anhydrous 85
Potassium Phosphate, Monobasic, Anhydrous 85 Zinc Sulfate7H2O 5.3
Zinc Sulfate7H2O 5.3 Activated Charcoal 2000
Activated Charcoal 2000 Agar 8000
Agar 7000 myo-Inositol 100
Banana Powder 30,000 MESH2O 1000
myo-Inositol 100 Nicotinic Acid 1.0
MESH2O 1000 Peptone from Meat 2000
Nicotinic Acid 1.0 PyridoxineHCl 1.0
Peptone from Meat 2000 Sucrose 20,000
PyridoxineHCl 1.0 ThiamineHCl 10
Sucrose 20,000 Approximate pH at Room Temperature 5.25 0.5
ThiamineHCl 10
Available Package Sizes
Approximate pH at Room Temperature 5.0 0.5
1L 10L 50L 100L
Available Package Sizes
1L 10L 50L

Plant Tissue Culture Media A-Z Page 113


Product Catalogue & Laboratory Guide
P668 - Orchid Maintenance Medium O753 - Orchid Multiplication Medium
Contains Charcoal (Product Number C325), Without Agar Contains Agar (Product Number A111), Without Charcoal
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water (Partially) Soluble in Water (Partially)
Use at 27.31 grams per liter Use at 32.30 grams per liter
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Nitrate 825 Ammonium Nitrate 825
Boric Acid 3.1 Boric Acid 3.1
Calcium Chloride, Anhydrous 166 Calcium Chloride, Anhydrous 166
Cobalt Chloride6H2O 0.0125 Cobalt Chloride6H2O 0.0125
Cupric Sulfate5H2O 0.0125 Cupric Sulfate5H2O 0.0125
Na2EDTA2H2O 37.3 Na2EDTA2H2O 37.3
Ferrous Sulfate7H2O 27.85 Ferrous Sulfate7H2O 27.85
Plant Tissue Culture Media

Magnesium Sulfate, Anhydrous 90.35 Magnesium Sulfate, Anhydrous 90.35


Manganese SulfateH2O 8.45 Manganese SulfateH2O 8.45
Molybdic Acid, Disodium Salt2H2O 0.125 Molybdic Acid, Disodium Salt2H2O 0.125
Potassium Iodide 0.415 Potassium Iodide 0.415
Potassium Nitrate 950 Potassium Nitrate 950
Potassium Phosphate, Monobasic, Anhydrous 85 Potassium Phosphate, Monobasic, Anhydrous 85
Zinc Sulfate7H2O 5.3 Zinc Sulfate7H2O 5.3
Activated Charcoal 2000 Agar 7000
myo-Inositol 100 6-Benzylaminopurine 2.0
MESH2O 1000 myo-Inositol 100
Nicotinic Acid 1.0 MESH2O 1000
Peptone from Meat 2000 -Naphthaleneacetic Acid 0.5
PyridoxineHCl 1.0 Nicotinic Acid 0.5
Sucrose 20,000 Peptone from Meat 2000
ThiamineHCl 10 PyridoxineHCl 0.5
Approximate pH at Room Temperature 5.25 0.5 Sucrose 20,000
Available Package Sizes ThiamineHCl 1.0
1L 10L 50L 100L Approximate pH at Room Temperature 5.25 0.5
Available Package Sizes
Selecting Terrestrial Orchid Media by Genus
1L 10L 50L
See "Figure 24. Terrestrial Orchid Media Selection Guide by
Genus" on page 251 in the Technical Section.

Find Phytopathology Media on Pages 161 to 164


Oat Meal Agar
Czapex & Dox Agar & Broth
Bacterial Screening Media
Potato Dextrose Agar & Broth
Corn Meal Agar

Page 114 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
P793 - Orchid Multiplication Medium P723 - Orchid Seed Sowing Medium
Without Charcoal and Agar Contains Charcoal (Product Number C325) and Agar (Product
Store at 2 to 6 C Number A111)
Soluble in Water Store at 2 to 6 C
Use at 25.30 grams per liter Soluble in Water (Partially)
Plant Tissue Culture Tested Use at 32.74 grams per liter
Components (mg/L) Plant Tissue Culture Tested
Ammonium Nitrate 825 Components (mg/L)
Boric Acid 3.1 Ammonium Nitrate 412.5
Calcium Chloride, Anhydrous 166 Boric Acid 1.65
Cobalt Chloride6H2O 0.0125 Calcium Chloride, Anhydrous 83
Cupric Sulfate5H2O 0.0125 Cobalt Chloride6H2O 0.00625
Na2EDTA2H2O 37.3 Cupric Sulfate5H2O 0.00625
Ferrous Sulfate7H2O 27.85 Na2EDTA2H2O 18.65

Plant Tissue Culture Media


Magnesium Sulfate, Anhydrous 90.35 Ferrous Sulfate7H2O 13.93
Manganese SulfateH2O 8.45 Magnesium Sulfate, Anhydrous 75.18
Molybdic Acid, Disodium Salt2H2O 0.125 Manganese SulfateH2O 4.23
Potassium Iodide 0.415 Molybdic Acid, Disodium Salt2H2O 0.0625
Potassium Nitrate 950 Potassium Iodide 0.2075
Potassium Phosphate, Monobasic, Anhydrous 85 Potassium Nitrate 475
Zinc Sulfate7H2O 5.3 Potassium Phosphate, Monobasic, Anhydrous 42.5
6-Benzylaminopurine 2.0 Zinc Sulfate7H2O 2.65
myo-Inositol 100 Activated Charcoal 1000
MESH2O 1000 Agar 8000
-Naphthaleneacetic Acid 0.5 myo-Inositol 100
Nicotinic Acid 0.5 MESH2O 500
Peptone from Meat 2000 Nicotinic Acid 1.0
PyridoxineHCl 0.5 Peptone from Meat 2000
Sucrose 20,000 PyridoxineHCl 1.0
ThiamineHCl 1.0 Sucrose 20,000
Approximate pH at Room Temperature 5.25 0.5 ThiamineHCl 10
Approximate pH at Room Temperature 5.75 0.5
Available Package Sizes
1L 10L 50L Available Package Sizes
1L 10L 50L

Plant Tissue Culture Media A-Z Page 115


Product Catalogue & Laboratory Guide
P727 - Orchid Seed Sowing Medium II P713 - Parker Thompson Fern Basal Salt Mixture
Contains Agar (Product Number A111), Without Charcoal Contains the macro- and micronutrients as described by
Store at 2 to 6 C Hickok et al. (1995).
Soluble in Water (Partially) Store at 2 to 6 C
Use at 31.74 grams per liter Soluble in Water
Plant Tissue Culture Tested Use at 0.77 grams per liter
Components (mg/L) Plant Tissue Culture Tested
Ammonium Nitrate 412.5 Components (mg/L)
Boric Acid 1.65 Ammonium Molybdate(VI)4H2O 0.037
Calcium Chloride, Anhydrous 83 Ammonium Nitrate 125
Cobalt Chloride6H2O 0.0063 Boric Acid 1.86
Cupric Sulfate5H2O 0.0063 Calcium Chloride, Anhydrous 19.628
Na2EDTA2H2O 18.65 Cupric Sulfate5H2O 0.37
Ferrous Sulfate7H2O 13.93 Na2EDTA2H2O 37.3
Plant Tissue Culture Media

Magnesium Sulfate, Anhydrous 75.18 Ferrous Sulfate7H2O 27.8


Manganese SulfateH2O 4.23 Magnesium Sulfate, Anhydrous 58.565
Molybdic Acid, Disodium Salt2H2O 0.0625 Manganese SulfateH2O 0.25
Potassium Iodide 0.2075 Potassium Phosphate, Monobasic, Anhydrous 500
Potassium Nitrate 475 Zinc Sulfate7H2O 0.52
Potassium Phosphate, Monobasic, Anhydrous 42.5 Approximate pH at Room Temperature 4.0 0.5
Zinc Sulfate7H2O 2.65 Available Package Sizes
Agar 8000 1L 10L 50L
myo-Inositol 100
Peptone Waters
MESH2O 500
See Microbiology Section for Complete Listings
Nicotinic Acid 1.0
Peptone from Meat 2000 New Product
PyridoxineHCl 1.0 P6700 - Phyto-Organic Potato Media Kit
Sucrose 20,000 Proprietary media formulation. Kit contains sufficient
ThiamineHCl 10 Phyto-Organic Organic Plant Growth Regulators (OPGR)
Approximate pH at Room Temperature 5.5 0.5 Liquid Supplements for amount of media purchased. OPGR
Supplements are allotted as one-liter applications.
Available Package Sizes
1L 10L 50L Phyto-Organic Media are manufactured from chemical
components sourced from the Organic Material Review
Institutes (OMRI) list of organic chemicals.
For use in laboratory research only.
Store at 2 to 6 C
Soluble in Water
Use at 8.71 grams per liter of medium
Plant Tissue Culture Tested
pH at Room Temperature 5.7 1.0
Available Package Sizes
1L 10L

Page 116 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
New Product P785 - PhytoTech Orchid Replate Medium II
P7000 - Phyto-Organic Potato Media II Kit Proprietary Formulation
Contains Sucrose, Banana Powder, and a Gelling Agent
Proprietary media formulation. Kit contains sufficient
Does not contain Activated Charcoal
Phyto-Organic Organic Plant Growth Regulators (OPGR)
A complete orchid replate and seed sowing medium.
Liquid Supplements for amount of media purchased. OPGR
Supplements are allotted as one-liter applications. Store at 2 to 6 C
Soluble in Water (Partially)
Phyto-Organic Media are manufactured from chemical Use at 65.79 grams per liter
components sourced from the Organic Material Review Plant Tissue Culture Tested
Institutes (OMRI) list of organic chemicals. Approximate pH at Room Temperature 4.75 0.5
For use in laboratory research only.
Available Package Sizes
Store at 2 to 6 C
1L 10L 50L
Soluble in Water
Use at 8.88 grams per liter of medium P656 - PhytoTech Phalaenopsis Replate Medium
Plant Tissue Culture Tested
Proprietary Formulation
pH at Room Temperature 5.7 1.0

Plant Tissue Culture Media


Contains Sucrose, Banana Powder, Potato Powder, Activated
Available Package Sizes Charcoal, and a gelling agent.
1L 10L A complete orchid replate medium.
Store at 2 to 6 C
P6800 - PhytoSelect Basal Medium Soluble in Water (Partially)
See Seed Testing Section for Complete Listing Use at 61.31 grams per liter
Plant Tissue Culture Tested
P781 - PhytoTech Orchid Replate Medium Approximate pH at Room Temperature 5.5 0.5
Proprietary Formulation
Available Package Sizes
Contains Sucrose
Does not contain Banana Powder, Activated Charcoal, or a 1L 10L 50L
Gelling Agent
An orchid replate and seed sowing medium.
P6647 - PhytoTech Spathiphyllum
Multiplication Medium
Store at 2 to 6 C
Soluble in Water A complete medium for the micropropagation of
Use at 35.81 grams per liter Spathiphyllum. Contains gelling agent, carbohydrates, and plant
Plant Tissue Culture Tested growth regulators.
PhytoTech Spathiphyllum Multiplication Medium is a
Approximate pH at Room Temperature 4.75 0.5
proprietary plant cell culture medium that was originally
Available Package Sizes developed for the culture of foliage plants such as
1L 10L 50L Spathiphyllum.
Store at 2 to 6 C
P782 - PhytoTech Orchid Replate Medium Soluble in Water
Proprietary Formulation Use at 33.85 grams per liter
Contains Sucrose and a Gelling Agent Plant Tissue Culture Tested
Does not contain Banana Powder or Activated Charcoal Available Package Sizes
An orchid replate and seed sowing medium.
1L 10L 50L
Store at 2 to 6 C
Soluble in Water (Partially) Potato Dextrose Broth & Agar
Use at 43.81 grams per liter
See Phytopathology Section for Complete Listings
Plant Tissue Culture Tested
Approximate pH at Room Temperature 5.0 0.5
Available Package Sizes
1L 10L 50L

Epiphytic Orchid Media Selection Guide


See "Figure 23. Epiphytic Orchid Media Selection Guide" on
page 250 in the Technical Section.

Plant Tissue Culture Media A-Z Page 117


Product Catalogue & Laboratory Guide
Q673 - Quoirin & Lepoivre Basal Salt Mixture R756 - Rose Modified Initiation Basal Medium
Contains the macro- and micronutrients as described by Stage I
Quoirin & Lepoivre (1977) and Quoirin et al. (1977). Contains Ferric Sodium EDTA in place of both Ferrous Sulfate
Store at 2 to 6 C and Disodium EDTA.
Soluble in Water Store at 2 to 6 C
Use at 3.56 grams per liter Soluble in Water
Plant Tissue Culture Tested Use at 4.51 grams per liter
Components (mg/L) Plant Tissue Culture Tested
Ammonium Nitrate 400 Components (mg/L)
Boric Acid 6.2 Ammonium Nitrate 1650
Calcium Nitrate 833.77 Boric Acid 6.2
Cobalt Chloride6H2O 0.025 Calcium Chloride, Anhydrous 333
Cupric Sulfate5H2O 0.025 Cobalt Chloride6H2O 0.025
Na2EDTA2H2O 37.3 Cupric Sulfate5H2O 0.025
Ferric Sodium EDTA 36.7
Plant Tissue Culture Media

Ferrous Sulfate7H2O 27.8


Magnesium Sulfate, Anhydrous 175.79 Magnesium Sulfate, Anhydrous 181
Manganese SulfateH2O 0.76 Manganese SulfateH2O 16.9
Molybdic Acid, Disodium Salt2H2O 0.25 Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Iodide 0.08 Potassium Iodide 0.83
Potassium Nitrate 1800 Potassium Nitrate 1900
Potassium Phosphate, Monobasic, Anhydrous 270 Potassium Phosphate, Monobasic, Anhydrous 170
Zinc Sulfate7H2O 8.6 Zinc Sulfate7H2O 8.6
Approximate pH at Room Temperature 4.0 0.5 L-Ascorbic Acid 50
6-Benzylaminopurine 2.0
Available Package Sizes
Citric Acid, Anhydrous 50
1L 10L 50L
Glycine 2.0
Indole-3-acetic Acid 0.3
myo-Inositol 100
Nicotinic Acid 0.5
PyridoxineHCl 0.5
ThiamineHCl 0.4
Approximate pH at Room Temperature 3.5 0.5
Available Package Sizes
1L 10L 50L

For Find Media for the Various Stages of


Tissue Culture Plant Growth
See "Figure 5. Examples of Media Designed for a Specific Stage
of Growth" on page 231 in the Technical Section.

Page 118 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
R757 - Rose Modified Multiplication Basal R758 - Rose Modified Rooting Basal Medium
Medium Stage III
Stage II Contains Ferric Sodium EDTA in place of both Ferrous Sulfate
Contains Ferric Sodium EDTA in place of both Ferrous Sulfate and Disodium EDTA.
and Disodium EDTA. Store at 2 to 6 C
Store at 2 to 6 C Soluble in Water
Soluble in Water Use at 1.18 grams per liter
Use at 4.51 grams per liter Plant Tissue Culture Tested
Plant Tissue Culture Tested Components (mg/L)
Components (mg/L) Ammonium Nitrate 412.5
Ammonium Nitrate 1650 Boric Acid 1.55
Boric Acid 6.2 Calcium Chloride, Anhydrous 83.25
Calcium Chloride, Anhydrous 333 Cobalt Chloride6H2O 0.00625
Cobalt Chloride6H2O 0.025 Cupric Sulfate5H2O 0.00625
Cupric Sulfate5H2O 0.025 Ferric Sodium EDTA 9.175

Plant Tissue Culture Media


Ferric Sodium EDTA 36.7 Magnesium Sulfate, Anhydrous 45.25
Magnesium Sulfate, Anhydrous 181 Manganese SulfateH2O 4.725
Manganese SulfateH2O 16.9 Molybdic Acid, Disodium Salt2H2O 0.0625
Molybdic Acid, Disodium Salt2H2O 0.25 Potassium Iodide 0.2075
Potassium Iodide 0.83 Potassium Nitrate 475
Potassium Nitrate 1900 Potassium Phosphate, Monobasic, Anhydrous 42.5
Potassium Phosphate, Monobasic, Anhydrous 170 Zinc Sulfate7H2O 2.15
Zinc Sulfate7H2O 8.6 Glycine 2.0
L-Ascorbic Acid 50 myo-Inositol 100
6-Benzylaminopurine 3.0 -Naphthaleneacetic Acid 0.03
Citric Acid, Anhydrous 50 Nicotinic Acid 0.5
Glycine 2.0 PyridoxineHCl 0.5
Indole-3-acetic Acid 0.30 ThiamineHCl 0.4
myo-Inositol 100 Approximate pH at Room Temperature 5.0 0.5
Nicotinic Acid 0.5 Available Package Sizes
PyridoxineHCl 0.5 1L 10L 50L
ThiamineHCl 0.4
Approximate pH at Room Temperature 3.5 0.5
Available Package Sizes
1L 10L 50L

Find Microbiology Media on Pages 151 to 159


LB Agars and Broths
Tryptic Soy Broth
YEP Media
Hanahans Broth
Peptone Waters

Plant Tissue Culture Media A-Z Page 119


Product Catalogue & Laboratory Guide
R7100 - Rugini Olive Medium S813 - Schenk & Hildebrandt Basal Medium
Contains the Macronutrients, Micronutrients, and Vitamins as Contains 10 g/L Sucrose (Product Number S391)
described by Rugini (1984). Contains the macro- and micronutrients and vitamins as
Store at 2 to 6 C described by Schenk & Hildebrandt (1972).
Soluble in Water Store at 2 to 6 C
Use at 4.05 grams per liter of medium Soluble in Water
Plant Tissue Culture Tested Use at 14.21 grams per liter
Components (mg/L) Plant Tissue Culture Tested
Ammonium Nitrate 412 Components (mg/L)
Boric Acid 12.4 Ammonium Phosphate, Monobasic 300
Calcium Chloride, Anhydrous 332.16 Boric Acid 5
Calcium Nitrate, anhydrous 416.9 Calcium Chloride, Anhydrous 151
Cobalt Chloride, hexahydrate 0.025 Cobalt Chloride6H2O 0.1
Cupric Sulfate, pentahydrate 0.25 Cupric Sulfate5H2O 0.2
Na2 EDTA2H2O 20
Plant Tissue Culture Media

Disodium EDTA dihydrate 37.5


Ferrous Sulfate, heptahydrate 27.8 Ferrous Sulfate7H2O 15
Magnesium Sulfate, anhydrous 732.5 Magnesium Sulfate, Anhydrous 195.4
Manganese Sulfate, monohydrate 16.9 Manganese SulfateH2O 10
Molybdic Acid, sodium salt dihydrate 0.25 Molybdic Acid, Disodium Salt2H2O 0.1
Potassium Chloride 500 Potassium Iodide 1
Potassium Nitrate 2500
Potassium Iodide 0.83
Zinc Sulfate7H2O 1
Potassium Nitrate 1100
myo-Inositol 1000
Potassium Phosphate, monobasic 340
Nicotinic Acid 5
Zinc Sulfate, heptahydrate 14.3
PyridoxineHCI 0.5
D-Biotin 0.05
Sucrose 10,000
Folic Acid 0.5
ThiamineHCI 5
Glycine 2
Approximate pH at Room Temperature 4.25 0.5
myo-Inositol 100
Nicotinic Acid 5
Available Package Sizes
1L 10L 50L
Pyridoxine HCl 0.5
Thiamine HCl 0.5 S826 - Schenk & Hildebrandt Vitamin Powder
Approximate pH at Room Temperature 4.5 0.5 (100x)
Available Package Sizes Contains the vitamins as described by Schenk & Hildebrandt
1L 10L 50L (1972).
Store at 2 to 6 C
S7536 - Sabouraud Dextrose Broth Soluble in Water
See Microbiology Section for Complete Listing Use 10.11 grams to make 100 mL
Use at 10.0 mL per liter of Medium
Plant Tissue Culture Tested
Components (mg/L)
myo-Inositol 100,000
Nicotinic Acid 500
PyridoxineHCl 50
ThiamineHCl 500
Approximate pH at Room Temperature 4.0 0.75
Available Package Sizes
100mL 1L

Page 120 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
S808 - Schenk & Hildebrandt Modified Basal S811 - Schenk & Hildebrandt Modified Basal
Medium (x) Medium
Contains 10 g/L Sucrose Contains 10 g/L Sucrose (Product Number S391)
Contains the macro- and micronutrients and the vitamins Contains the macro- and micronutrients as described by Schenk
as described by Schenk & Hildebrandt (1972). & Hildebrandt (1972); without vitamins.
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water Soluble in Water
Use at 12.10 grams per liter Use at 13.20 grams per liter
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Phosphate, Monobasic 150 Ammonium Phosphate, Monobasic 300
Boric Acid 2.5 Boric Acid 5.0
Calcium Chloride, Anhydrous 75.5 Calcium Chloride, Anhydrous 151
Cobalt Chloride6H2O 0.05 Cobalt Chloride6H2O 0.1
Cupric Sulfate5H2O 0.1 Cupric Sulfate5H2O 0.2

Plant Tissue Culture Media


Na2 EDTA2H2O 10 Na2 EDTA2H2O 20
Ferrous Sulfate7H2O 7.5 Ferrous Sulfate7H2O 15
Magnesium Sulfate, Anhydrous 97.7 Magnesium Sulfate, Anhydrous 195.4
Manganese SulfateH2O 5.0 Manganese SulfateH2O 10
Molybdic Acid, Disodium Salt2H2O 0.05 Molybdic Acid, Disodium Salt2H2O 0.1
Potassium Iodide 0.5 Potassium Iodide 1.0
Potassium Nitrate 1250 Potassium Nitrate 2500
Zinc Sulfate7H2O 0.5 Zinc Sulfate7H2O 1.0
myo-Inositol 500 Sucrose 10,000
Nicotinic Acid 2.5 Approximate pH at Room Temperature 4.25 0.5
PyridoxineHCI 0.25 Available Package Sizes
Sucrose 10,000 1L 10L 50L
ThiamineHCI 2.5
Approximate pH at Room Temperature 4.5 0.5
Available Package Sizes
1L 10L 50L

Find Biochemicals on Pages 15 to 56


Gelling Agents
Plant Growth Regulators
Media Components
Stains and Dyes
Carbohydrates

Plant Tissue Culture Media A-Z Page 121


Product Catalogue & Laboratory Guide
S806 - Schenk & Hildebrandt Modified Basal Salt S657 - SOC Medium Solution
Mixture Without Calcium See Microbiology Section for Complete Listing
Contains the macro- and micronutrients as described by Schenk
& Hildebrandt (1972); without Calcium Chloride. S7478 - Sodaria Crossing Agar
Store at 2 to 6 C See Microbiology Section for Complete Listing
Soluble in Water
S743 - Staba Modified Vitamin Solution (100x)
Use at 3.05 grams per liter
Plant Tissue Culture Tested Contains a modification of the Staba (1969) vitamins as
described by Skirvin & Chu (1979). This modification differs
Components (mg/L)
from the original Staba formulation in that
Ammonium Phosphate, Monobasic 300 myo-Inositol was reduced from 2000 mg/L to 100 mg/L.
Boric Acid 5.0 Store at -20 to 0 C
Cobalt Chloride6H2O 0.1 Sterile Filtered
Cupric Sulfate5H2O 0.2 Miscible with Water
Use at 10.0 mL per liter of Medium
Na2 EDTA2H2O 20
Plant Tissue Culture Tested
Plant Tissue Culture Media

Ferrous Sulfate7H2O 15
Components (mg/L)
Magnesium Sulfate, Anhydrous 195.4
p-Aminobenzoic Acid 50
Manganese SulfateH2O 10
D-Biotin 100
Molybdic Acid, Disodium Salt2H2O 0.1
Calcium Pantothenate 100
Potassium Iodide 1.0
Choline Chloride 100
Potassium Nitrate 2500
Cyanocobalamin 0.15
Zinc Sulfate7H2O 1.0
Folic Acid 50
Approximate pH at Room Temperature 4.25 0.5
myo-Inositol 10,000
Available Package Sizes Niacinamide 200
1L 10L 50L PyridoxineHCl 200
S816 - Schenk & Hildebrandt Basal Salt Mixture Riboflavin 50
Contains the macro- and micronutrients as described by Schenk ThiamineHCl 100
& Hildebrandt (1972). Approximate pH at Room Temperature 6.0 0.5
Store at 2 to 6 C Available Package Sizes
Soluble in Water 100mL 500mL
Use at 3.20 grams per liter
Plant Tissue Culture Tested S7668 - Sueokas High-Salt Medium (HSM)
Components (mg/L) See Phycology Section for Complete Listing
Ammonium Phosphate, Monobasic 300
Boric Acid 5.0
Calcium Chloride, Anhydrous 151
Cobalt Chloride6H2O 0.1
Cupric Sulfate5H2O 0.2
Na2 EDTA2H2O 20
Ferrous Sulfate7H2O 15
Magnesium Sulfate, Anhydrous 195.4
Manganese SulfateH2O 10
Molybdic Acid, Disodium Salt2H2O 0.1
Potassium Iodide 1.0
Potassium Nitrate 2500
Zinc Sulfate7H2O 1.0
Approximate pH at Room Temperature 4.5 0.5
Available Package Sizes
1L 10L 10LFB 50L 100L

Page 122 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
T839 - Terrestrial (Cypripedium) Orchid Medium T849 - Terrestrial (Cypripedium) Orchid Medium
Contains 400 mg/L Calcium Nitrate (Product Number C180) Contains 400 mg/L Calcium Nitrate (Product Number C180)
and 400 mg/L Casein (Product Number C184) Without Casein
Without Ammonium Nitrate Mother Flasking Medium V
Contains a modification of the macro- and micronutrients, Contains the macro- and micronutrients, Glucose, and Agar as
Glucose, and Agar as described by Steele (1996). described by Steele (1996).
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water (Partially) Soluble in Water (Partially)
Use at 27.44 grams per liter Use at 28.44 grams per liter
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Citrate, Dibasic 19 Ammonium Citrate, Dibasic 19
Boric Acid 0.5 Ammonium Nitrate 1400
Calcium Nitrate 400 Boric Acid 0.5
Cupric Sulfate5H2O 0.025 Calcium Nitrate 400

Plant Tissue Culture Media


Ferric Ammonium Citrate 25 Cupric Sulfate5H2O 0.025
Magnesium Sulfate, Anhydrous 97.69 Ferric Ammonium Citrate 25
Manganese SulfateH2O 1.54 Magnesium Sulfate, Anhydrous 97.69
Molybdic Acid, Diodium Salt2H2O 0.02 Manganese SulfateH2O 1.54
Potassium Chloride 100 Molybdic Acid, Disodium Salt2H2O 0.02
Potassium Iodide 0.1 Potassium Chloride 100
Potassium Nitrate 200 Potassium Iodide 0.1
Potassium Phosphate, Monobasic, Anhydrous 200 Potassium Nitrate 200
Zinc Sulfate7H2O 0.5 Potassium Phosphate, Monobasic, Anhydrous 200
Agar 6000 Zinc Sulfate7H2O 0.5
Casein, Enzymatic Hydrolysate 400 Agar 6000
D-Glucose, Anhydrous 20,000 D-Glucose, Anhydrous 20,000
Approximate pH at Room Temperature 5.5 0.5 Approximate pH at Room Temperature 5.25 0.5
Available Package Sizes Available Package Sizes
1L 10L 50L 1L 10L 50L

Tissue Culture Kits


PhytoTechnology Laboratories
offers a selection of plant tissue
culture kits for educational
use. For more information
please see pages 179 to 185.

Kits for:
African Violet,
Carrot,
Carnivorous Plants,
Fern,
Hosta,
Lily,
Orchids, and
Potato.

Plant Tissue Culture Media A-Z Page 123


Product Catalogue & Laboratory Guide
T842 - Terrestrial (Cypripedium) Orchid Medium T7954 - TM4 Basal Medium
Contains 600 mg/L Calcium Nitrate (Product Number C180) Contains the Macro- and Micronutrients and Vitamins as
and 200 mg/L Casein (Product Number C184) described by Shahin (1984).
Without Ammonium Nitrate Originally developed for work with tomato protoplasts.
Contains a modification of the macro- and micronutrients, Store at 2 to 6 C
Glucose, and Agar as described by Steele (1996). Soluble in Water
Store at 2 to 6 C Use at 2.99 grams per liter of medium
Soluble in Water (Partially) Plant Tissue Culture Tested
Use at 27.44 grams per liter Components (mg/L)
Plant Tissue Culture Tested
Ammonium Nitrate 320
Components (mg/L)
Ammonium Phosphate, Monobasic 230
Ammonium Citrate, Dibasic 19
Ammonium Sulfate 134
Boric Acid 0.5
Boric Acid 6.2
Calcium Nitrate 600
Calcium Chloride, Anhydrous 113.25
Cupric Sulfate5H2O 0.025
Cobalt Chloride6H2O 0.025
Plant Tissue Culture Media

Ferric Ammonium Citrate 25


Cupric Sulfate5H2O 0.025
Magnesium Sulfate, Anhydrous 97.69
Na2 EDTA2H2O 18.5
Manganese SulfateH2O 1.54
Ferrous Sulfate7H2O 13.9
Molybdic Acid, Disodium Salt2H2O 0.02
Magnesium Sulfate, Anhydrous 122.12
Potassium Chloride 100
Manganese SulfateH2O 16.9
Potassium Iodide 0.1
Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Nitrate 200
Potassium Iodide 0.83
Potassium Phosphate, Monobasic, Anhydrous 200
Potassium Nitrate 1900
Zinc Sulfate7H2O 0.5
Zinc Sulfate7H2O 8.6
Agar 6000
D-Biotin 0.05
Casein, Enzymatic Hydrolysate 200
Choline Chloride 0.10
D-Glucose, Anhydrous 20,000
Folic Acid 0.5
Approximate pH at Room Temperature 5.25 0.5
Glycine (Free Base) 2.5
Available Package Sizes
myo-Inositol 100
1L 10L 50L
Nicotinic Acid 5
Terrific Broths PyridoxineHCl 0.5
See Microbiology Section for Complete Listings ThiamineHCl 0.5
Approximate pH at Room Temperature 4.5 0.5
Available Package Sizes
1L 10L 50L

Page 124 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
T8024 - TM4 Basal Salt Mixture T868 - TM4G Basal Medium
Contains the Macro- and Micronutrients and Vitamins as Store at 2 to 6 C
described by Shahin (1984). Soluble in Water
Originally developed for work with tomato protoplasts. Use at 2.99 grams per liter
Store at 2 to 6 C Plant Tissue Culture Tested
Soluble in Water Components (mg/L)
Use at 2.88 grams per liter of medium Ammonium Nitrate 320
Plant Tissue Culture Tested
Ammonium Phosphate, Monobasic 230
Components (mg/L)
Ammonium Sulfate 130
Ammonium Nitrate 320
Boric Acid 6.2
Ammonium Phosphate, Monobasic 230
Calcium Chloride, Anhydrous 113.25
Ammonium Sulfate 134
Cobalt Chloride6H2O 0.025
Boric Acid 6.2
Cupric Sulfate5H2O 0.025
Calcium Chloride, Anhydrous 113.25
Na2EDTA2H2O 18.65
Cobalt Chloride6H2O 0.025

Plant Tissue Culture Media


Ferrous Sulfate7H2O 13.9
Cupric Sulfate5H2O 0.025
Magnesium Sulfate, Anhydrous 122.12
Na2EDTA2H2O 18.5
Manganese SulfateH2O 16.9
Ferrous Sulfate7H2O 13.9 Molybdic Acid, Disodium Salt2H2O 0.25
Magnesium Sulfate, Anhydrous 122.12 Potassium Iodide 0.83
Manganese SulfateH2O 16.9 Potassium Nitrate 1900
Molybdic Acid, Disodium Salt2H2O 0.25 Zinc Sulfate7H2O 9.2
Potassium Iodide 0.83 D-Biotin 0.05
Potassium Nitrate 1900 Folic Acid 0.5
Zinc Sulfate7H2O 8.6 Glycine 2.5
Approximate pH at Room Temperature 4.25 0.5 myo-Inositol 100
Available Package Sizes Nicotinic Acid 5.0
1L 10L 50L PyridoxineHCl 0.5
ThiamineHCl 0.5
Approximate pH at Room Temperature 4.25 0.5
Available Package Sizes
1L 10L 50L

Plant Tissue Culture Media A-Z Page 125


Product Catalogue & Laboratory Guide
T853 - TM4G Basal Salt Mixture T856 - Tobacco Modified Callus Initiation Basal
Store at 2 to 6 C Medium
Soluble in Water Contains Ferric Sodium EDTA in place of both Ferrous Sulfate
Use at 2.88 grams per liter and Disodium EDTA.
Plant Tissue Culture Tested
Store at 2 to 6 C
Components (mg/L) Soluble in Water
Ammonium Nitrate 320 Use at 5.41 grams per liter
Ammonium Phosphate, Monobasic 230 Plant Tissue Culture Tested
Ammonium Sulfate 130 Components (mg/L)
Boric Acid 6.2 Ammonium Nitrate 1650
Calcium Chloride, Anhydrous 113.25 Boric Acid 6.2
Cobalt Chloride6H2O 0.025 Calcium Chloride, Anhydrous 333
Cupric Sulfate5H2O 0.025 Cobalt Chloride6H2O 0.025
Na2EDTA2H2O 18.65 Cupric Sulfate5H2O 0.025
Plant Tissue Culture Media

Ferrous Sulfate7H2O 13.9 Ferric Sodium EDTA 36.7


Magnesium Sulfate, Anhydrous 122.12 Magnesium Sulfate, Anhydrous 181
Manganese SulfateH2O 16.9 Manganese SulfateH2O 16.9
Molybdic Acid, Disodium Salt2H2O 0.25 Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Iodide 0.83 Potassium Iodide 0.83
Potassium Nitrate 1900 Potassium Nitrate 1900
Zinc Sulfate7H2O 9.2 Potassium Phosphate, Monobasic, Anhydrous 170
Approximate pH at Room Temperature 4.25 0.5 Zinc Sulfate7H2O 8.6
Casein, Enzymatic Hydrolysate 1000
Available Package Sizes
Glycine 2.0
1L 10L 50L
Indole-3-acetic Acid 2.0
myo-Inositol 100
Kinetin 0.2
Nicotinic Acid 0.5
PyridoxineHCl 0.5
ThiamineHCl 0.4
Approximate pH at Room Temperature 5.5 1.0
Available Package Sizes
1L 10L 50L

Find Molecular Biology Products on Pages 131 to 150


Antibiotics
Buffers
DNA Extraction Kits
Sterile Solutions
IPTG, X-Gal & X-Gluc

Page 126 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
T867 - Tobacco Modified Shoot & Root Basal T864 - Tobacco Modified Shoot Multiplication
Medium Basal Medium
Contains Ferric Sodium EDTA in place of both Ferrous Sulfate Contains Ferric Sodium EDTA in place of both Ferrous Sulfate
and Disodium EDTA. and Disodium EDTA.
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water Soluble in Water
Use at 5.41 grams per liter Use at 5.41 grams per liter
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Nitrate 1650 Ammonium Nitrate 1650
Boric Acid 6.2 Boric Acid 6.2
Calcium Chloride, Anhydrous 333 Calcium Chloride, Anhydrous 333
Cobalt Chloride6H2O 0.025 Cobalt Chloride6H2O 0.025
Cupric Sulfate5H2O 0.025 Cupric Sulfate5H2O 0.025

Plant Tissue Culture Media


Ferric Sodium EDTA 36.7 Ferric Sodium EDTA 36.7
Magnesium Sulfate, Anhydrous 181 Magnesium Sulfate, Anhydrous 181
Manganese SulfateH2O 16.9 Manganese SulfateH2O 16.9
Molybdic Acid, Disodium Salt2H2O 0.25 Molybdic Acid, Disodium Salt2H2O 0.25
Potassium Iodide 0.83 Potassium Iodide 0.83
Potassium Nitrate 1900 Potassium Nitrate 1900
Potassium Phosphate, Monobasic, Anhydrous 170 Potassium Phosphate, Monobasic, Anhydrous 170
Zinc Sulfate7H2O 8.6 Zinc Sulfate7H2O 8.6
Casein, Enzymatic Hydrolysate 1000 Casein, Enzymatic Hydrolysate 1000
Glycine 2.0 Glycine 2.0
Indole-3-acetic Acid 0.03 myo-Inositol 100
myo-Inositol 100 Kinetin 1.0
Kinetin 1.0 Nicotinic Acid 0.5
Nicotinic Acid 0.5 PyridoxineHCI 0.5
PyridoxineHCl 0.5 ThiamineHCI 0.4
ThiamineHCl 0.4 Approximate pH at Room Temperature 5.5 1.0
Approximate pH at Room Temperature 5.5 1.0 Available Package Sizes
Available Package Sizes 1L 10L 50L
1L 10L 50L

Plant Tissue Culture Media A-Z Page 127


Product Catalogue & Laboratory Guide
T861 - Tobacco Modified Root Initiation Basal V505 - Vacin & Went Modified Orchid Basal
Medium Salt Mixture
Contains Ferric Sodium EDTA in place of both Ferrous Sulfate Contains the macro- and micronutrients as described by
and Disodium EDTA. Vacin & Went (1949) modified with an equivalent Iron molar
Store at 2 to 6 C concentration of Ferrous Sulfate in place of Ferric Tartrate.
Soluble in Water Store at 2 to 6 C
Use at 5.41 grams per liter Soluble in Water
Plant Tissue Culture Tested Use at 1.67 grams per liter
Components (mg/L) Plant Tissue Culture Tested
Ammonium Nitrate 1650 Components (mg/L)
Boric Acid 6.2 Ammonium Sulfate 500
Calcium Chloride, Anhydrous 333 Calcium Phosphate, Tribasic 200
Cobalt Chloride6H2O 0.025 Na2EDTA2H2O 37.26
Cupric Sulfate5H2O 0.025 Ferrous Sulfate7H2O 27.8
Magnesium Sulfate, Anhydrous 122.1
Plant Tissue Culture Media

Ferric Sodium EDTA 36.7


Magnesium Sulfate, Anhydrous 181 Manganese SulfateH2O 5.6
Manganese SulfateH2O 16.9 Potassium Nitrate 525
Molybdic Acid, Disodium Salt2H2O 0.25 Potassium Phosphate, Monobasic, Anhydrous 250
Potassium Iodide 0.83 Approximate pH at Room Temperature 5.75 0.5
Potassium Nitrate 1900 Available Package Sizes
Potassium Phosphate, Monobasic, Anhydrous 170 1L 10L 50L
Zinc Sulfate7H2O 8.6
Selecting Epiphytic Orchid Media by Genus
Casein, Enzymatic Hydrolysate 1000
See "Figure 25. Epiphytic Orchid Media Selection Guide by
Glycine 2.0 Genus" on page 252 in the Technical Section.
Indole-3-acetic Acid 3.0
myo-Inositol 100 V882 - Vacin & Went Modified Orchid Basal
Nicotinic Acid 0.5 Medium
PyridoxineHCl 0.5 Without Sucrose
Contains the macro- and micronutrients as described by
ThiamineHCl 0.4
Vacin & Went (1949) modified with an equivalent Iron molar
Approximate pH at Room Temperature 5.5 1.0 concentration of Ferrous Sulfate in place of Ferric Tartrate. Also
Available Package Sizes contains (mg/L): 0.4 Thiamine.
1L 10L 50L Store at 2 to 6 C
Soluble in Water
Tryptic Soy Broths Use at 1.67 grams per liter
See Microbiology Section for Complete Listings Plant Tissue Culture Tested
Components (mg/L)
T8050 - Tris-Acetate-Phosphate (TAP) Ammonium Sulfate 500
1x Solution Calcium Phosphate, Tribasic 200
See Phycology Section for Complete Listing
Na2EDTA2H2O 37.26
T8224 - Tris-Acetate-Phosphate (TAP) Ferrous Sulfate7H2O 27.8
See Phycology Section for Complete Listing Magnesium Sulfate, Anhydrous 122.1
Manganese SulfateH2O 5.6
Potassium Nitrate 525
Potassium Phosphate, Monobasic, Anhydrous 250
ThiamineHCl 0.4
Approximate pH at Room Temperature 5.75 0.5
Available Package Sizes
1L 10L 50L

Page 128 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
V895 - Vacin & Went Modified Orchid Medium W863 - Westvaco WV3 Basal Medium
Contains Agar (Product Number A111) & Sucrose (Product Contains the macro- and micronutrients and vitamins as
Number S391) described by Coke (1996).
Contains the macro- and micronutrients as described by Store at 2 to 6 C
Vacin & Went (1949) modified with an equivalent Iron molar
Soluble in Water
concentration of Ferrous Sulfate in place of Ferric Tartrate. Also
contains: 0.4 mg/L Thiamine. Use at 4.29 grams per liter
Plant Tissue Culture Tested
Store at 2 to 6 C
Soluble in Water (Partially) Components (mg/L)
Use at 28.67 grams per liter Boric Acid 31
Plant Tissue Culture Tested Calcium Chloride, Anhydrous 452.88
Components (mg/L) Cobalt Chloride6H2O 0.025
Ammonium Sulfate 500 Cupric Sulfate5H2O 0.25
Calcium Phosphate, Tribasic 200 Ferric Sodium EDTA 36.71
Na2EDTA2H2O 37.26 Magnesium Sulfate, Anhydrous 903.79

Plant Tissue Culture Media


Ferrous Sulfate7H2O 27.8 Manganese SulfateH2O 15.16
Magnesium Sulfate, Anhydrous 122.1 Molybdic Acid, Disodium Salt2H2O 0.25
Manganese SulfateH2O 5.6 Potassium Chloride 656.79
Potassium Nitrate 525 Potassium Iodide 0.83
Potassium Phosphate, Monobasic, Anhydrous 250 Potassium Nitrate 910.06
Agar 7000 Potassium Phosphate, Monobasic, Anhydrous 270
Sucrose 20,000 Zinc Sulfate7H2O 8.6
ThiamineHCl 0.4 myo-Inositol 1000
Approximate pH at Room Temperature 5.75 0.5 ThiamineHCI 0.4
Available Package Sizes Approximate pH at Room Temperature 4.75 0.5
1L 10L 50L Available Package Sizes
1L 10L 50L
V891 - Vacin & Went Modified Orchid Medium
Contains Sucrose (Product Number S391)
Contains the macro- and micronutrients as described by Vacin
and Went (1949) modified with an equivalent Iron molar
concentration of Ferrous Sulfate in place of Ferric Tartrate. Also
contains (mg/L): 0.4 Thiamine.
Store at 2 to 6 C
Soluble in Water
Use at 21.67 grams per liter
Plant Tissue Culture Tested
Components (mg/L)
Ammonium Sulfate 500
Calcium Phosphate, Tribasic 200
Na2 EDTA2H2O 37.26
Ferrous Sulfate7H2O 27.8
Magnesium Sulfate, Anhydrous 122.1
Manganese SulfateH2O 5.6
Potassium Nitrate 525
Potassium Phosphate, Monobasic, Anhydrous 250
Sucrose 20,000
ThiamineHCl 0.4
Approximate pH at Room Temperature 5.75 0.5
Available Package Sizes
1L 10L 50L

Plant Tissue Culture Media A-Z Page 129


Product Catalogue & Laboratory Guide
W865 - Westvaco WV5 Basal Medium W898 - White Basal Salt Mixture
Contains the macro- and micronutrients and vitamins as Contains the macro- and micronutrients and vitamins as
described by Coke (1996). described by White (1963).
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water Soluble in Water
Use at 5.22 grams per liter Use at 0.93 grams per liter
Plant Tissue Culture Tested Plant Tissue Culture Tested
Components (mg/L) Components (mg/L)
Ammonium Nitrate 700 Boric Acid 1.5
Boric Acid 31 Calcium Nitrate 208.5
Calcium Chloride, Anhydrous 452.88 Cupric Sulfate5H2O 0.001
Cobalt Chloride6H2O 0.025 Ferric SulfatexH2O 2.5
Cupric Sulfate5H2O 0.25 Magnesium Sulfate, Anhydrous 351.62
Ferric Sodium EDTA 36.71 Manganese SulfateH2O 5.31
Plant Tissue Culture Media

Magnesium Sulfate, Anhydrous 903.79 Molybdenum Trioxide 0.0001


Manganese SulfateH2O 15.16 Potassium Chloride 65
Molybdic Acid, Disodium Salt2H2O 0.25 Potassium Iodide 0.75
Potassium Chloride 718.67 Potassium Nitrate 80
Potassium Iodide 0.83 Sodium Phosphate, MonobasicH2O 16.5
Potassium Nitrate 1084.06 Sodium Sulfate, Anhydrous 200
Potassium Phosphate, Monobasic, Anhydrous 270 Zinc Sulfate7H2O 3.0
Zinc Sulfate7H2O 8.6 Approximate pH at Room Temperature 4.75 0.75
myo-Inositol 1000 Available Package Sizes
ThiamineHCl 0.4 1L 10L 50L
Approximate pH at Room Temperature 4.75 0.5
X8454 - XTS Medium
Available Package Sizes
See Seed Testing Section for Complete Listing
1L 10L 50L
YEP Media
W887 - Wilkins-Chalgren Agar
See Microbiology Section for Complete Listings
See Microbiology Section for Complete Listing
Y893 - YMB Medium
See Microbiology Section for Complete Listing

Y8488 - Yeast Malt Broth


See Microbiology Section for Complete Listing

Y8565 - YEP Broth


See Microbiology Section for Complete Listing

Page 130 2015 PhytoTechnology Laboratories


Product Catalogue & Laboratory Guide
Molecular Biology Featured in this section
PhytoTechnology Laboratories offers a Antibiotics
wide variety of products for use in plant
molecular biology. The products listed
in this section are those that are most Antibiotic Solutions
commonly used in various molecular
biology applications. For bacteria growth
media please refer to the Microbiology Buffers
section on page 151 and the
Phytopathology section on page 161.
Transformation
If you have trouble finding a product
please consult the indices at the back Stains & Dyes
of this catalogue. Be sure to check back
at www.phytotechlab.com for new
products! Reagents
Molecular Biology

Sterile Antibiotic Solutions


PhytoTechnology Laboratories offers
And more...
a line of ready-to-use, sterile-filtered
antibiotic solutions for use in molecular
biology. Each solution is manufactured in-
house and then sterile-filtered at the main
campus of PhytoTechnology Laboratories.
All sterile filtered liquids are confirmed
sterile according to USP<71> protocols.

Many of our sterile antibiotic solutions are


offered, for your convenience, in packages
of ten 1mL aliquots. Sterile antibiotic
solutions are not only convenient and
easy-to-use, but also allow the use of
antibiotics without the need for a full
body respirator. For more information
about antibiotics, see "Antibiotic
Selection, Preparation, & Storage" on
page 257.

Page 132 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
A104 - Acetosyringone A116 - Ampicillin, Sodium Salt
4-Hydroxy-3,5-dimethoxyacetophenone USP Grade, D(-)--Aminobenzylpenicillin, Sodium Salt
Plant Tissue Culture Tested Powder
This material is sensitive to temperature and should not be CAS Number: 69-52-3
autoclaved. Formula: C16H18N3NaO4S
Powder Molecular Weight: 371.4
CAS Number: 2478-38-8 Soluble in Water, DMSO
Formula: C10H12O4 Store at 2 to 6 C
Molecular Weight: 196.20 Merck 13, 591
Soluble in DMSO Available Package Sizes
Store at Room Temperature 5g 25g
Available Package Sizes
1g 5g 25g
A1116 - Ampicillin Solution (100 mg/mL)
D(-)--Aminobenzylpenicillin, Sodium Salt in DMSO
A1104 - Acetosyringone 100 mM Solution Liquid
4-Hydroxy-3,5-dimethoxyacetophenone in DMSO CAS Number: 69-52-3
Plant Tissue Culture Tested Formula: C16H18N3NaO4S
This material is sensitive to temperature and should not be Molecular Weight: 371.4
autoclaved. Miscible with Water

Molecular Biology
Liquid Store at -20 to 0 C
CAS Number: 2478-38-8 Ships on dry ice
Formula: C10H12O4
Molecular Weight: 196.20
Miscible with Water Available Package Sizes
Store at -20 to 0 C
10x1mL 10mL 25mL 100mL
Ships on dry ice
Available Package Sizes A119 - Amphotericin B
10x1mL 10mL 25mL Powder
CAS Number: 1397-89-3
A1315 - Agarose, Low Gelling Temp, SeaPlaque Formula: C47H73NO17
See Biochemicals Section for Complete Listing Molecular Weight: 924.09
Soluble in DMSO
A1050 - LE Agarose Store at 2 to 6 C
For gel electrophoresis of nucleic acids. Merck 13, 590
Gelling Temperature: >33C for 1.5% gels Available Package Sizes
Gelling Strength (1% is > 1200 g/cm2)
500mg 1g 5g
Powder
CAS Number: 9012-36-6 A122 - Amoxicillin Trihydrate
Formula: (C12H14O5(OH)4)n (2S,5R,6R)-6-[[(2R)-Amino(4-hydroxyphenyl)acetyl]amino]
Low EEO -3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-
Melting Temperature: 87.0 1.5C for 1.5% gels carboxylic Acid Trihydrate
Molecular Weight: Variable
Powder
Soluble in Hot Water
CAS Number: 61336-70-7
Store at Room Temperature
Formula: C16H19N3O5S3H2O
Available Package Sizes Molecular Weight: 419.5
10g 100g 500g 1Kg Forms Suspension with Water
Store at 2 to 6 C
Merck 13, 582
Available Package Sizes
1g 5g 25g

B129 - Bacteria Screening Medium 523


See Phytopathology Section for Complete Listing

Molecular Biology A-Z Page 133


Product Catalogue & Laboratory Guide
B132 - Bacitracin Zinc X884 - Blue-Gal
Powder 5-Bromo-3-indolyl--D-galactopyranoside
CAS Number: 1405-89-6 Powder
Formula: C66H101N17O16SZn CAS Number: 97753-82-7
Molecular Weight: 1486.07 Formula: C14H16BrNO6
Soluble in Water at 5.1 mg/mL (28 C) Merck Molecular Weight: 374.2
Store at 2 to 6 C Soluble in DMSO or KOH
Merck 13, 938 Store at -20 to 0 C
Available Package Sizes Available Package Sizes
1g 5g 25g 100mg 500mg 1g
B131 - Bialaphos B441 - Bradford Reagent
A tripeptide herbicidal antibiotic that can be used as a selection Used to determine concentration of proteins in solution based
agent with the bar and pat genes for transformation research. on the formation of a Brilliant Blue G - protein complex.
Powder Useful in determining the protein content of samples and for
CAS Number: 71048-99-2 gel electrophoresis.
Formula: C11H21N3NaO6P Liquid
Molecular Weight: 345.27 Store at 2 to 6 C
Soluble in Water
Molecular Biology

Store at -20 to 0 C
Available Package Sizes
Available Package Sizes
10mL 100mL
100mg 500mg 1g
C1888 - Carbendazim
B1730 - Bialaphos Solution (1mg/mL)
BCM, Methyl benzimidazol-2-ylcarbamate, methyl-1H-
A tripeptide herbicidal antibiotic that can be used as a selection benzimidazol-2-yl carbamate
agent with the bar and pat genes. Plant Tissue Culture Tested
Sterile Filtered
Powder
Liquid CAS Number: 10605-21-7
CAS Number: 71048-99-2 Formula: C9H9N3O2
Formula: C11H21N3NaO6P Molecular Weight: 191.19
Molecular Weight: 345.27 Soluble in HCl at 10 mg/mL
Miscible with Water Store at 2 to 6 C
Store at -20 to 0 C
Available Package Sizes
Available Package Sizes 1g 5g 25g
10x1mL 25mL 100mL
C346 - Carbenicillin, Disodium Salt
B162 - BIS-TRIS -Carboxybenzylpenicillin, Disodium Salt
2,2-Bis-(hydroxymethyl)-2,2',2"-nitrilotriethanol Plant Tissue Culture Tested
Plant Tissue Culture Tested
Powder
Useful pH range: 5.8-7.2
CAS Number: 4800-94-6
pKa @ 25C = 6.5
Formula: C17H16N2O6SNa2
Powder Molecular Weight: 422.41
CAS Number: 6976-37-0 Soluble in Water
Formula: C8H19NO5 Store at 2 to 6 C
Molecular Weight: 209.2 Merck 13, 1801
Soluble in Water
Store at Room Temperature Available Package Sizes
5g 10g 25g 100g
Available Package Sizes
100g 500g 1Kg

Page 134 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
C540 - Carbenicillin Solution (100 mg/mL) C537 - Cefotaxime Solution (100 mg/mL)
-Carboxybenzylpenicillin, Disodium Salt Aqueous Solution
Aqueous Solution Sterile Filtered
Sterile Filtered Plant Tissue Culture Tested
Plant Tissue Culture Tested Liquid
Liquid CAS Number: 64485-93-4
CAS Number: 4800-94-6 Formula: C16H16N5O7S2Na
Formula: C17H16N2O6SNa2 Molecular Weight: 477.4
Molecular Weight: 422.41 Miscible with Water
Miscible with Water Store at -20 to 0 C
Store at -20 to 0 C Ship on dry ice
Ship on dry ice
Available Package Sizes
Available Package Sizes 10x1mL 10mL 100mL
10x1mL 10mL 25mL 100mL
C1880 - Cefotaxime Solution (250 mg/mL)
C2046 - Carbenicillin Solution (250 mg/mL) Aqueous Solution
-Carboxybenzylpenicillin, Disodium Salt Sterile Filtered
Aqueous Solution Plant Tissue Culture Tested
Sterile Filtered Liquid

Molecular Biology
Plant Tissue Culture Tested CAS Number: 64485-93-4
Liquid Formula: C16H16N5O7S2Na
CAS Number: 4800-94-6 Molecular Weight: 477.4
Formula: C17H16N2O6SNa2 Miscible with Water
Molecular Weight: 422.41 Store at -20 to 0 C
Miscible with Water Ships on dry ice
Store at -20 to 0 C Available Package Sizes
Ships on dry ice
10x1mL 10mL 25mL 100mL
Available Package Sizes
10x1mL 10mL 25mL 100mL C526 - CHAPS
3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
For More Information about Antibiotics
CHAPS is a nondenaturing zwitterionic detergent that is
See "Figure 30. Antibiotics/Antimycotics/Selection Agent typically used to solubilize membrane proteins.
Guide" on page 258 in the Technical Section.
Powder
C380 - Cefotaxime, Sodium Salt CAS Number: 75621-03-3
Formula: C32H58N2O7S
(6R,7R)-3-[(Acetyloxy)methyl]-7-[[(2Z)-(2-amino-4-thiazolyl)
Molecular Weight: 614.89
(methoxyimino)acetyl]amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]
Soluble in Water
oct-2-ene-2-carboxylic Acid, Sodium Salt
Store at Room Temperature
Plant Tissue Culture Tested
Merck 13, 2052
Powder
CAS Number: 64485-93-4 Available Package Sizes
Formula: C16H16N5O7S2Na 1g 5g 10g
Molecular Weight: 477.4
Soluble in Water
Store at 2 to 6 C
Merck 13, 1946
Available Package Sizes
1g 5g 25g 100g

Molecular Biology A-Z Page 135


Product Catalogue & Laboratory Guide
C252 - Chloramphenicol H276 - CTAB
2,2-Dichloro-N-[(1R,2R)2-hydroxy-1-(hydroxymethyl)-2-(4- Hexadecyltrimethylammonium Bromide, HTAB, Centimide,
nitrophenyl)ethyl]acetamide Cetrimide, Cetrimonium bromide,
Plant Tissue Culture Tested N,N,N-trimethyl-1-hexadecanaminium bromide
Powder Molecular Biology Grade
CAS Number: 56-75-7 Powder
Formula: C11H12Cl2N2O5 CAS Number: 59-09-0
Molecular Weight: 323.13 Formula: CH3(CH2)15N(Br)(CH3)3
Soluble in Water at 2.5 mg/mL per tje Merck Molecular Weight: 364.45
Index. Soluble in Water
Store at Room Temperature Store at Room Temperature
Merck 13, 2087
Available Package Sizes
Available Package Sizes 100g 500g
100mg 5g 25g 100g
H3818 - CTAB Solution (100 mg/mL)
C2010 - Chloramphenicol Solution (10 mg/mL) Hexadecyltrimethylammonium Bromide, HTAB, Centimide,
2,2-Dichloro-N-[(1R,2R)2-hydroxy-1-(hydroxymethyl)-2-(4- Cetrimide, Cetrimonium bromide,
nitrophenyl)ethyl]acetamide N,N,N-trimethyl-1-hexadecanaminium bromide.
Sterile Filtered solution in 95% Ethanol An aqueous solution of CTAB (H276) at 100 milligrams per
Molecular Biology

Plant Tissue Culture Tested milliliter. A cationic detergent with bactericidal activity against
Liquid both gram-positive and gram-negative organisms.
CAS Number: 56-75-7 Liquid
Formula: C11H12Cl2N2O5 Sterile Filtered
Molecular Weight: 323.13 CAS Number: 57-09-0
Miscible with Water Formula: C19H42NBr
Store at -20 to 0 C Molecular Weight: 364.45
Ships on dry ice Miscible withWater
Store at Room Temperature

Available Package Sizes


10x1mL 10mL 25mL 100mL Available Package Sizes
10mL 100mL
C375 - Cupric Sulfate
See Biochemicals Section for Complete Listing C1989 - Cycloheximide
Actidione, Naramycin A
C466 - Copper(II) Sulfate Solution (4%) 3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]
Copper(II) Sulfate Pentahydrate glutarimide
Typically used with Bicinchoninic Acid Solution (B496) in the An antimycotic derived from Streptomyces griseus, which is active
determination of protein concentration. against many fungi, molds and yeasts; can be used as a plant
Liquid growth regulator, since it induces ethylene production in plants.
CAS Number: 7758-99-8 Powder
Formula: CuSO45H2O CAS Number: 66-81-9
Molecular Weight: 249.68 Formula: C15H23NO4
Miscible with Water Molecular Weight: 281.4
Store at Room Temperature Soluble in Water, DMSO, Ethanol
Merck 13, 2682 Store at 2 to 6 C
Available Package Sizes Available Package Sizes
100mL 500mL 1L 1g 5g 25g

Page 136 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
C1796 - Cycloheximide Solution (100 mg/mL) D241 - Dimethyl Sulfoxide
A solution of Cycloheximide at 100 milligrams per milliliter. DMSO; Methyl Sulfoxide
Cycloheximide is also known as Actidione, Naramycin A Plant Tissue Culture Tested
3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl] Solidifies below and melts above 18.5C.
glutarimide
Liquid
An antimycotic derived from Streptomyces griseus, which is active
CAS Number: 67-68-5
against many fungi, molds and yeasts; can be used as a plant
Formula: (CH3)2SO
growth regulator, since it induces ethylene production in plants.
Molecular Weight: 78.13
Liquid Miscible with Water
CAS Number: 66-81-9 Store at Room Temperature
Formula: C15H23NO4 Merck 13, 3285
Molecular Weight: 281.4
Miscible with Water Available Package Sizes
Store at 2 to 6 C 500mL 1L 4L
Available Package Sizes D548 - DNA Denaturing Solution
10x1mL 10mL 25mL Contains 1.5 M Sodium Chloride (87.66 g/L) and 0.5 M
Sodium Hydroxide (20.0 g/L)
P679 - DL-Phosphinothricin, Monoammonium Salt
Sterile Filtered
PPT; 2-Amino-4-(hydroxymethylphosphinyl)butanoic Acid,

Molecular Biology
Liquid
Monoammonium Salt; Glufosinate Ammonium
Miscible with Water
Powder Store at Room Temperature
CAS Number: 77182-82-2
Formula: C5H15N2O4P
Molecular Weight: 198.19
Soluble in Water Available Package Sizes
Store at Room Temperature 100mL 500mL 1L
Merck 13, 7425
Available Package Sizes DNA Extraction System for Plant Genomic DNA
Viogene Plant Genomic DNA Extraction System
250mg 1g
Isolation of up to 50kb of Plant Genomic Material with
G523 - DL-Phosphinothricin Solution (1 mg/mL) predominant fragments of 20-30 kb, final yield dependant on
kit type.
PPT; 2-Amino-4-(hydroxymethylphosphinyl)butanoic Acid, Tissue is ground with liquid N2, Incubated with RNAse,
Monoammonium Salt; Glufosinate Ammonium lysate is sheared, gDNA is captured on silica-gel membrane.
Aqueous Solution Isolation of gDNA (viral, mitochondial, and chloroplast)
Sterile Filtered from 1g of plant tissue (MaxiPrep) or 0.1g of plant tissue
Liquid (MiniPrep).
CAS Number: 77182-82-2 Purities typically range from A260/A280 = 1.7-1.9
Formula: C5H15N2O4P D2251 (MiniPrep Kit), D2275 (MaxiPrep Kit, Pictured
Molecular Weight: 198.19 Below)
Miscible with Water
Store at 2 to 6 C
Available Package Sizes
10mL 100mL

Product Number Isolation Capacity Available Package Sizes


D2251 Up to 40g 50 Preps 250 Preps
D2275 Up to 1000g 20 Preps

Molecular Biology A-Z Page 137


Product Catalogue & Laboratory Guide
DNA Extraction System for Plasmid DNA DNA Ladders
Great kit for cloning plasmid pieces that will be cut-out and Viogene VioEasy DNA Ladders
ligated into vectors. Linear dsDNA with blunt-ends.
Downstream applications: Automated or Manual Sequencing, Pictured below are both DNA ladders in 2% LE Agarose
Restriction analysis, Cloning, and Transfection of most cell (Product Number A1050).
lines. Preps per kit 166 preps in D2300, 167 preps in D2450.
Viogene Plasmid DNA Extraction System D2300 D2450
DNA able to be processed at capacity dependant on kit type,
which generally corresponds to 1-5 mL of E. Coli culture
(OD600=0.9-1.5).
Silica-gel membrane technology that binds up to 40g of
Plasmid DNA in the presence of chaotropic salts (MiniPlus Kit).
Elution into low-salt buffer so that it doesnt impact
sequencing, transfection, etc. (MiniPlus Kit).
Diethylaminoethyl (DEAE) ligands bound to macropus
silica resin for anion exchange in gravity flow. (MidiPlus and
MaxiPlus kits)
D2126 (MiniPlus Kit), D2179 (MidiPlus Kit), D2231
(MaxiPlus Kit, Pictured Below)
Molecular Biology

Product Number Ladder Size Lane Amount Package Sizes


D2300 100 bp 300 ng 50g/0.5mL
D2450 1 kb 400 ng 67g/0.5mL

D544 - DNA Neutralizing Solution, pH 7.4


Sterile Filtered
DNAse/RNAse Free
Product Number Processing Capacity Available Package Sizes
Liquid
D2126 > 0.04mg 50 Preps 250 Preps Miscible with Water
D2179 > 0.1mg 25 Preps 50 Preps Store at Room Temperature
D2231 > 0.5mg 10 Preps 25 Preps Components (g/L)
Tris HCl (0.84 M) 132.20
Tris Base (0.16 M) 19.40
Sodium Chloride (1.5 M) 87.66
Contains sufficient HCl to adjust pH to 7.4
Available Package Sizes
500mL 1L

Page 138 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
E316 - Ethylenediaminetetraacetic Acid, Free Acid G810 - G418 Sulfate
EDTA; Edetic Acid Geneticin
Plant Tissue Culture Tested Geneticin is a registered trademark of Invitrogen Corporation.
Powder Powder
CAS Number: 60-00-4 CAS Number: 108321-42-2
Formula: C10H16N2O8 Formula: C20H40N4O10 2H2SO4
Molecular Weight: 292.24 Molecular Weight: 692.7
Soluble in KOH Soluble in Water
Store at Room Temperature Store at 2 to 6 C
Merck 13, 3546
Available Package Sizes
Available Package Sizes 1g 5g 10g
100g 500g 1Kg

E582 - EDTA Solution 0.5 M, pH 8.0 G570 - Gentamicin Sulfate


Sterile Filtered Gentamycin Sulfate
DNAse/RNAse Free Powder
Liquid CAS Number: 1405-41-0
CAS Number: 6381-92-6 Formula: C21H43N5O7 H2SO4
Formula: C10H14N2O8Na22H2O Molecular Weight: 575.67

Molecular Biology
Molecular Weight: 372.24 Soluble in Water
Miscible with Water Store at 2 to 6 C
Store at Room Temperature Merck 13, 4403
Merck 13, 3543 Available Package Sizes
Components (g/L) 5g 10g 25g
Na2EDTA2H2O (0.5 M) 186.12
Contains sufficient NaOH to adjust pH to 8.0
G3410 - Gentamicin Solution (100 mg/mL)
Gentamycin Sulfate
Available Package Sizes Aqueous Solution
500mL 1L Sterile Filtered

E344 - Erythromycin Liquid


CAS Number: 1405-41-0
USP Grade Formula: C21H43N5O7 H2SO4
Erythromycin A Molecular Weight: 575.67
Powder Miscible with Water
CAS Number: 114-07-8 Store at 2 to 6 C
Formula: C37H67NO13
Molecular Weight: 733.92 Available Package Sizes
Soluble in EtOH at 2mg/mL per the Merck 10mL 25mL 100mL
Index
Store at Room Temperature G3350 - Gentamicin Solution (50 mg/mL)
Merck 13, 3714 Gentamycin Sulfate
Aqueous Solution
Available Package Sizes
Sterile Filtered
5g 25g
Liquid
CAS Number: 1405-41-0
Formula: C21H43N5O7 H2SO4
Molecular Weight: 575.67
Miscible with Water
Store at 2 to 6 C
Available Package Sizes
10mL 25mL 100mL

G3574 - Guaiacol
See Seed Testing Section for Complete Listing

Molecular Biology A-Z Page 139


Product Catalogue & Laboratory Guide
G3300 - Guanidine Thiocyanate New Product
Guanidinium rhodanide or Guanidinium thiocyanate. X8475 - GUS Staining Solution without X-Gluc (1x)
A denaturing and chaotropic agent often used in buffers for Solution contains biochemical reagents used in GUS gene
nucleic acid extractions. identification models using the presence of -galactosidase.
Powder
CAS Number: 593-84-0 This solution does not include X-Gluc, a necessary component of
Formula: CH5N3 HSCN the GUS Staining Solution. See either product number X8451 -
Molecular Weight: 118.16 X-Gluc Solution (20 mg/mL) or product number X877 - X-Gluc,
Soluble in Water Monocyclohexyl Ammonium Salt.
Store at Room Temperature Store at -20 to 0 C
Available Package Sizes Sterile Filtered
100g 500g 1kg Adjusted to pH 7.0
Miscible with Water
New Product Light Sensitive
G3600 - GUS Staining Kit
Kit contains biochemical reagents used in GUS gene Components (mg/mL)
identification models using the presence of -galactosidase. Sodium Phosphate, Dibasic 10.37
Sodium Phosphate, Monobasic 8.46
Molecular Biology

Kit contains two solutions that when combined will provide EDTA, Disodium Salt 0.37
100mL of completed GUS Staining Solution.
Potassium Ferricyanide 0.16
This Kit Contains:
Potassium Ferrocyanide 0.21
Product Package Product Quantity
Triton X-100 0.1% v/v
Number Size Description
X8451 5 mL X-Gluc Solution (20 mg/mL) 1 Available Package Sizes
X8475 95 mL GUS Staining Solution 1 95mL 475mL
without X-Gluc (1x)
H289 Hanahans Broth
Instruction Manual 1
See Microbiology Section for Complete Listing
Available Package Sizes
1 Kit H326 - HEPES, Free Acid
N-(2-Hydroxyethyl)Piperazine-N-(2-Ethanesulfonic Acid);
4-(2-Hydroxyethyl)-1-Piperazineethanesulfonic Acid
Molecular Biology Grade
DNAse/RNAse Free
pKa 7.5 (25 C)
Effective Buffering Range: pH 6.8 to 8.2
Powder
CAS Number: 7365-45-9
Formula: C8H18N2O4S
Molecular Weight: 238.3
Soluble in Water
Store at Room Temperature
Available Package Sizes
100g 500g 1Kg

Page 140 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
H245 - Hydrochloric Acid Solution 1.0 N H370 - Hygromycin B Solution, 50 mg/mL in PBS
Hydrogen Chloride Solution 1.0 N; Muriatic Acid Buffer
Plant Tissue Culture Tested O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-
Contains about 3.06% (w/v) concentrated Hydrochloric Acid (12-3)-O--D-talopyranosyl-(15)-2-deoxy-N3-methyl-D-
per liter Streptamine
Liquid Sterile Filtered
CAS Number: 7647-01-0 From Streptomyces hygroscopicus
Formula: HCl Liquid
Molecular Weight: 36.46 CAS Number: 31282-04-9
Miscible with Water Formula: C20H37N3O13
Store at Room Temperature Molecular Weight: 527.5
Merck 13, 4801 Miscible with Water or PBS Buffer
Store at 2 to 6 C; Do Not Freeze
Available Package Sizes
Available Package Sizes
10x1mL 20mL
500mL 1L 4L
I373 - Isopropyl--D-thiogalactopyranoside
H397 - Hygromycin B
IPTG
O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-

Molecular Biology
Powder
(12-3)-O--D-talopyranosyl-(15)-2-deoxy-N3-methyl-D-
CAS Number: 367-93-1
Streptamine
Formula: C9H18O5S
From Streptomyces hygroscopicus
Molecular Weight: 238.3
Powder Soluble in Water
CAS Number: 31282-04-9 Store at 2 to 6 C
Formula: C20H37N3O13
Molecular Weight: 527.5 Available Package Sizes
Soluble in Water or PBS Buffer 1g 5g 25g
Store at 2 to 6 C
Merck 13, 4878 K378 - Kanamycin Monosulfate
Available Package Sizes O-3-Amino-3-deoxy--D-glucopyranosyl-(16)-O-[6-amino-
6-deoxy--D-glucopyranosyl-(14)]-2-deoxy-D-Streptamine
1g 5g Monosulfate
Plant Tissue Culture Tested
H385 - Hygromycin B Solution, 100 mg/mL in
Powder
Distilled Water
CAS Number: 25389-94-0
O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene- Formula: C18H36N4O11 H2SO4
(12-3)-O--D-talopyranosyl-(15)-2-deoxy-N3-methyl-D- Molecular Weight: 582.6
Streptamine Soluble in Water
Sterile Filtered Store at Room Temperature
From Streptomyces hygroscopicus Merck 13, 5299
Liquid
Available Package Sizes
CAS Number: 31282-04-9
Formula: C20H37N3O13 5g 10g 25g
Molecular Weight: 527.5
Miscible with Water or PBS Buffer
Store at 2 to 6 C; Do Not Freeze
Available Package Sizes
10x1mL 10mL

Molecular Biology A-Z Page 141


Product Catalogue & Laboratory Guide
K4751 - Kanamycin Monosulfate M649 - -Mercaptoethanol
Solution (100 mg/mL) 2-Mercaptoethanol, 2-Me, 2-Thioethanol, Thioglycol,
Sterile Filtered 2-Hydroxyethylmercaptan
Aqueous Solution Molecular Biology Grade
Plant Tissue Culture Tested DNase/RNase Free
Liquid Liquid
CAS Number: 25389-94-0 CAS Number: 60-24-2
Formula: C18H16N4O11 H2SO4 Formula: HSCH2CH2OH
Molecular Weight: 582.6 Molecular Weight: 78.13
Miscible with Water Miscible with Water
Store at 2 to 6 C Concentration: 14.3 M
Ship on Blue Ice Density: 1.114 g/mL at 20 C
Store at Room Temperature
Available Package Sizes
10mL 25mL 100mL
Available Package Sizes
K586 - Kanamycin Monosulfate Solution (50 mg/mL) Inquire about Package Size, Pricing, and more Information
Sterile Filtered
New Product
Aqueous Solution
M5600 - Meropenem Trihydrate
Molecular Biology

Plant Tissue Culture Tested


Liquid (1R,5S,6S)-2-[(3S,5S)-5-(dimethylaminocarbonyl)pyrrolidin-
CAS Number: 25389-94-0 3-ylthio]-6-[(R)-1-hydroxyethyl]-1-methylcarbapen-2-em-3-
Formula: C18H16N4O11 carboxylic acid trihydrate
Molecular Weight: 582.6 Powder
Miscible with Water CAS Number: 119478-56-7
Store at 2 to 6 C Formula: C17H25N3O5S 3H2O
Ship on Blue Ice Molecular Weight: 437.51
Available Package Sizes Soluble in DMSO
Store at -20C
10x1mL 10mL 25mL 100mL
Merck 13, 5926
K559 - Kasugamycin Hydrochloride Monohydrate Available Package Sizes
3-O-[2-amino-4-[(carboxyiminomethyl)amino]-2,3,4,6- 10mg 100mg 1g
tetradeoxy--D-arabino-hexopyranosyl]-D-chiro-inositol
Hydrochloride Monohydrate M825 - MES, Monohydrate
Plant Tissue Culture Tested 2-(N-Morpholino)ethanesulfonic Acid, Free Acid
Powder Plant Tissue Culture Tested
CAS Number: 6980-18-3 Useful pH range: 5.5 - 6.7
Formula: C14H25N3O9HClH2O pKa @ 25oC = 6.1
Molecular Weight: 433.8 Powder
Soluble in Water CAS Number: 145224-94-8
Store at 2 to 6 C Formula: C6H13NO4SH2O
Available Package Sizes Molecular Weight: 213.3
Soluble in Water
1g 5g 25g
Store at Room Temperature
LB Broths & Agars Merck 13, 5929

See Microbiology Section for Complete Listing Available Package Sizes


100g 500g 1Kg
Malt Extract Broth & Agar
See Microbiology Section for Complete Listing M5781 - Methylene Blue
See Seed Testing Section for Complete Listing

Page 142 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
M572 - MOPS-EDTA-Sodium Acetate Buffer N584 - Neomycin Sulfate
Solution (10x) Powder
MESA Buffer CAS Number: 1405-10-3
Sterile Filtered Formula: C23H46N6O13 3H2SO4
DNAse/RNAse Free Molecular Weight: 908.9
Soluble in Water
Liquid
Store at 2 to 6 C
Miscible with Water
Merck 13, 6482
Store at Room Temperature
Available Package Sizes
Components (g/L)
25g 100g
MOPS (0.4 M) 83.71
Sodium Acetate (0.1 M) 8.20 N5967 - Neomycin Solution (50 mg/mL)
Na2EDTA2H2O (0.01 M) 7.4 Sterile Filtered
Available Package Sizes Aqueous Solution
100mL 500mL 1L Liquid
CAS Number: 1405-10-3
M631 - MOPS, Free Acid Formula: C23H46N6O13 3H2SO4
Molecular Weight: 908.9
3-[N-Morpholino]propanesulfonic acid, Free Acid
Miscible with Water

Molecular Biology
Useful pH range: 6.5-7.9
Store at -20 to 0 C
DNAse/RNAse Tested
Ships on dry ice
pKa @ 25 C = 7.15
Powder
CAS Number: 1132-61-2
Formula: C7H15NO4S Available Package Sizes
Molecular Weight: 209.3 10mL 25mL 100mL
Soluble in Water
Store at Room Temperature N618 - 4-Nitrophenyl--D-glucuronide
Merck 13, 6288 PNPG
Available Package Sizes Powder
500g 1Kg CAS Number: 10344-94-2
Formula: C12H13NO9
M565 - MTT Molecular Weight: 315.2
3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium Bromide; Soluble in DMSO
Thiazolyl Blue Tetrazolium Bromide Store at -20 to 0 C
Powder Available Package Sizes
CAS Number: 298-93-1 250mg 1g
Formula: C18H16N5SBr
Molecular Weight: 414.32 N604 - 2-Nitrophenyl--D-galactopyranoside
Soluble in Water ONPG
Store at 2 to 6 C
Powder
Available Package Sizes CAS Number: 369-07-3
1g 5g 10g Formula: C12H15NO8
Molecular Weight: 301.3
M569 - MUG Soluble in DMSO
4-Methylumbelliferyl--D-glucuronide Trihydrate Store at -20 to 0 C
Powder Available Package Sizes
CAS Number: 6160-80-1 1g 5g 25g
Formula: C16H16O93H2O
Molecular Weight: 406.4
Soluble in DMSO
Store at -20 to 0 C
Available Package Sizes
100mg 1g

Molecular Biology A-Z Page 143


Product Catalogue & Laboratory Guide
N581 - Nystatin P711 - PIPES
USP Grade Piperazine-N,N-bis(2-ethanesulfonic Acid), Sesquisodium Salt
Powder Plant Tissue Culture Tested
CAS Number: 1400-61-9 Useful pH range: 6.1-7.5
Formula: C47H75NO17 pKa @ 25 C = 6.8
Molecular Weight: 926.12 Powder
Soluble in DMSO CAS Number: 100037-69-2
Store at 2 to 6 C Formula: C8H16.5N2O6S2Na1.5
Merck 13, 6770 Molecular Weight: 335.3
Soluble in Water
Available Package Sizes
Store at Room Temperature
5g 10g 25g Merck 13, 7561
O606 - Oatmeal Agar Available Package Sizes
See Phytopathology Section for Complete Listing 100g 500g 1Kg

P710 - Paromomycin Sulfate P671 - Potassium Acetate Solution 3.0 M, pH 5.0


O-2-Amino-2-deoxy--D-glucopyranosyl-(14)-O-[O- Sterile Filtered
2,6-diamino-2,6-dideoxy--L-idopyranosyl-(13)--D- Liquid
ribofuranosyl-(15)]-2-deoxy-D-streptamine sulfate CAS Number: 127-08-2
Molecular Biology

Plant Tissue Culture Tested Formula: KC2H3O2


Powder Molecular Weight: 98.14
CAS Number: 1263-89-4 Miscible with Water
Formula: C23H45N5O14H2SO4 Store at Room Temperature
Molecular Weight: 713.7 Merck 13, 7687
Soluble in Water Components (mg/L)
Store at Room Temperature
Potassium Acetate 294,420
Merck 13, 7113
Contains sufficient HCl to adjust pH to 5.0
Available Package Sizes
Available Package Sizes
5g 25g 100g
100mL 500mL 1L
P621 - Percoll
Percoll, Sterile
P730 - Potassium Chloride Solution 3.0 M
23% (w/w) aqueous solution of 15-30nm diameter colloidal Electrode Filling/ Soaking Solution
silica particles coated with polyvinylpyrrolidone (PVP). Liquid
Percoll is a registered trademark of GE Healthcare. CAS Number: 7447-40-7
Liquid Formula: KCl
Density: 1.130 0.005 g/mL Molecular Weight: 74.55
pH: 9.0 0.5 @ 20C Miscible with Water
Miscible with Water Store at Room Temperature
Store at 2 to 6 C Merck 13, 7704
Non-toxic Available Package Sizes
Available Package Sizes 100mL 500mL
25mL 100mL
How to Make a GUS Gene Staining Solution
P777 - Penicillin See "Figure 31. GUS Gene Staining Solution Formulation" on
See Seed Testing Section for Complete Listing page 260 in the Technical Section.

P6767 - Penicillin G Solution


See Seed Testing Section for Complete Listing

Peptone Waters
See Microbiology Section for Complete Listing

Page 144 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
P6626 - Potassium Ferricyanide R7153 - Plant RNA Extraction System
Also known as Potassium hexacyanoferrate(III), Iron Potassium Viogene VioTotal Plant RNA Mini
Cyanide, or Prussian Red Isolates RNA greater than 200nt (excluding 5.8S rRNA, 5S
Powder rRNA, and tRNA) from 50mg of plant tissue or 1x104 - 5x106
CAS Number: 13746-66-2 cells.
Formula: K3Fe(CN)6 Downstream applications include: Northern Blotting, RT-
Molecular Weight: 329.25 PCR, cDNA synthesis.
Soluble in Water The System accomodates secondary metabolites that have
Store at Room Temperature, Protect from light been known to gum/clog membranes.
Merck, 13, 7712 Purities typically range from A260/A280 = 1.9-2.1

Available Package Sizes


25g 100g 500g

P6826 - Potassium Ferrocyanide


Also known as Potassium Hexacyanoferrate(II) Trihydrate or
Yellow Prussiate.
Powder
CAS Number: 14459-95-1
Formula: K4Fe(CN)6 3H2O

Molecular Biology
Molecular Weight: 422.41
Soluble in Water
Store at Room Temperature
Merck 13, 7713 Available Package Sizes
Available Package Sizes 50 preps 250 preps
25g 100g 500g
New Product
P6780 - Potassium Sodium Tartrate S7777 - Silwet L-77
See Biochemicals Section for Complete Listing Also known as Alkoxylated trisilane.

R501 - Rifampicin Silwet L-77 is a non-ionic surfactant that is used in floral-dip


transformation with Agrobacterium tumefacians. It can also
3-[[(4-Methyl-1-piperazinyl)imino]methyl]rifamycin; Rifampin
be used as a wetting agent since it reduces the surface tension
Plant Tissue Culture Tested
of aqueous solutions. Silwet is a registered trademark of OSi
Powder Specialities.
CAS Number: 13292-46-1
Liquid
Formula: C43H58N4O12
CAS Number: 27306-78-1
Molecular Weight: 822.94
Formula: C13H34O4Si3
Soluble in DMSO
Molecular Weight: 338.66
Store at -20 to 0 C
Miscible with Water
Merck 13, 8300
Store at Room Temperature
Available Package Sizes Available Package Sizes
5g 10g 25g
10mL 25mL 100mL

S657 - SOC Medium Solution


See Microbiology Section for Complete Listing

Molecular Biology A-Z Page 145


Product Catalogue & Laboratory Guide
S844 - Sodium Dodecyl Sulfate S739 - Streptomycin Sulfate
SDS; Sodium Lauryl Sulfate USP Grade
Powder O-2-Deoxy-2-(methylamino)--L-glucopyranosyl-(12)
CAS Number: 151-21-3 -O-5-deoxy-3-C-formyl--L-lyxofuranosyl-(14)-N,N-
Formula: C12H25NaO4S bis(aminoiminomethyl)-D-streptamine Sulfate
Molecular Weight: 288.38 Powder
Soluble in Water CAS Number: 3810-74-0
Store at Room Temperature Formula: (C21H39N7O12)2 3H2SO4
Merck 13, 8710 Molecular Weight: 1457.4
Soluble in Water
Available Package Sizes
Store at 2 to 6 C
25g 100g 500g Merck 13, 8905
S742 - Spectinomycin Dihydrochloride Available Package Sizes
Togamycin; Trobicin 25g 100g
Powder
S7739 - Streptomycin Sulfate Solution (250 mg/mL)
CAS Number: 22189-32-8
Formula: C14H24N2O72HCl5H2O O-2-Deoxy-2-(methylamino)--L-glucopyranosyl-(12)
Molecular Weight: 495.35 -O-5-deoxy-3-C-formyl--L-lyxofuranosyl-(14)-N,N-
Soluble in Water bis(aminoiminomethyl)-D-streptamine Sulfate
Molecular Biology

Store at 2 to 6 C Aqueous Solution


Merck 13, 8813 Sterile Filtered
Liquid
Available Package Sizes
CAS Number: 3810-74-0
5g 25g Formula: (C21H39N7O12)2 3H2SO4
Molecular Weight: 1457.4
S716 - SSC Buffer Solution (20x), pH 7.0 Miscible with Water
Sterile Filtered Store at -20 to 0 C
DNAse/RNAse Free
Liquid
Available Package Sizes
Miscible with Water 10mL 25mL 100mL
Store at Room Temperature
Terrific Broths
Components (mg/L)
See Microbiology Section for Complete Listing
Sodium Chloride (3 M) 175,320
Sodium Citrate2H2O (0.3 M) 88,230 T859 - Tetracycline Hydrochloride
Contains sufficient HCl to adjust pH to 7.0 [4S-(4,4a,5a,6,12a)]-4-(Dimethylamino)-1,4,4a,5,5a,6-
11,12a-octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-
Available Package Sizes dioxo-2-napthacenecarboxamide Hydrochloride
500mL 1L Powder
CAS Number: 64-75-5
S677 - SSPE Buffer Solution (20x) Formula: C22H24N2O8HCl
Saline - Sodium Phosphate EDTA Buffer Solution Molecular Weight: 480.94
Sterile Filtered Soluble in Water
DNAse/RNAse Free Store at -20 to 0 C
Liquid Merck 13, 9271
Miscible with Water Available Package Sizes
Store at Room Temperature
5g 25g 100g
Components (g/L)
Sodium Chloride 175.32
Sodium Phosphate Monobasic 27.6
Na2EDTA2H2O 7.4
Contains sufficient amount of NaOH to adjust pH to 7.4 0.2
Available Package Sizes
100mL 500mL 1L

Page 146 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
T7859 - Tetracycline Hydrochloride T7869 - Timentin Solution (50 mg/mL)
Solution (10 mg/mL) Ticarcillin Disodium Salt/ Potassium Clavulanate Mixture (15:1)
[4S-(4,4a,5a,6,12a)]-4-(Dimethylamino)-1,4,4a,5,5a,6- Sterile Filtered
11,12a-octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11- Aqueous Solution
dioxo-2-napthacenecarboxamide Hydrochloride in DMSO Plant Tissue Culture Tested
Liquid Liquid
CAS Number: 64-75-5 CAS Number: 4697-14-7 / 61177-45-5
Formula: C22H24N2O8HCl Formula: C15H14N2Na2O6S2 / C8H8KNO5
Molecular Weight: 480.94 Molecular Weight: 428.4 / 237.3
Miscible with Water Miscible with Water
Store at -20 to 0 C Store at -20 to 0 C
Ships on dry ice Ships on dry ice
Available Package Sizes Available Package Sizes
10mL 25mL 100mL 10x1mL 10mL 25mL 100mL

For More Information about Antibiotics T834 - Tobramycin Sulfate


See "Figure 30. Antibiotics/Antimycotics/Selection Agent USP Grade
Guide" on page 258 in the Technical Section. O-3-Amino-3-deoxy--D-glucopyranosyl-(16)-O-[2,6-
diamino-2,3,6-trideoxy--D-ribo-hexopyranosyl-(14)]-2-

Molecular Biology
T869 - Timentin deoxy-D-Streptamine Sulfate
Ticarcillin Disodium Salt/ Potassium Clavulanate Powder
mixture (15:1) CAS Number: 79645-27-5
Plant Tissue Culture Tested Formula: (C18H37N5O9)2 5H2SO4
Powder Molecular Weight: 1425.4
CAS Number: 4697-14-7 / 61177-45-5 Soluble in Water
Formula: C15H14N2Na2O6S2 / C8H8KNO5 Store at 2 to 6 C
Molecular Weight: 428.4 / 237.3 Merck 13, 9567
Soluble in Water Available Package Sizes
Store at 2 to 6 C 1g 5g 25g
Available Package Sizes
2g 5g 10g 25g 100g
T769 - Tris Acetate EDTA Buffer Solution (50x),
pH 8.1 0.1
T767 - Timentin Solution (100 mg/mL) TAE Buffer used for DNA electrophoresis
Ticarcillin Disodium Salt/ Potassium Clavulanate Mixture (15:1) Sterile Filtered
Sterile Filtered DNAse/RNAse Free
Plant Tissue Culture Tested Liquid
Liquid Miscible with Water
CAS Number: 4697-14-7 / 61177-45-5 Store at Room Temperature
Formula: C15H14N2Na2O6S2 / C8H8KNO5 Components (g/L)
Molecular Weight: 428.4 / 237.3
Miscible with Water Tris Base 242.28
Store at -20 to 0 C EDTA, Free Acid 14.61
Ship on dry ice Glacial Acetic Acid 57.1 mL
Available Package Sizes Available Package Sizes
10x1mL 10mL 25mL 100mL 500mL 1L

For More Information on Preparing Tris Buffers


See "Figure 40. Tris Buffer Preparation Guide" on page 267 in
the Technical Section.

Molecular Biology A-Z Page 147


Product Catalogue & Laboratory Guide
T838 - Tris Base T773 - Tris Borate EDTA Buffer Solution (5x)
2-Amino-2-hydroxymethyl-1,3-propanediol TBE Buffer
Powder Sterile Filtered
CAS Number: 77-86-1 DNAse/RNAse Free
Formula: C4H11NO3 Liquid
Molecular Weight: 121.13 Miscible with Water
Soluble in Water Store at Room Temperature
Store at Room Temperature
Components (g/L)
Merck 13, 9842
Tris Base 53.9
Available Package Sizes
Boric Acid 27.5
100g 500g 1Kg 5Kg
Na2EDTA2H2O 3.7
T831 - Tris Borate EDTA Buffer Available Package Sizes
TBE Buffer 500mL 1L
Use 17.02 grams to make 1 liter of 1x buffer solution
Powder T855 - Tris-EDTA Buffer
Soluble in Water when pH = 8.5 TE Buffer
Store at Room Temperature Use 1.58 grams to make 1 liter of 1x buffer solution
Molecular Biology

Components (g/L) Powder


Soluble in Water
Tris Base 10.78
Store at Room Temperature
Boric Acid 5.5
Components (g/L)
Na2EDTA2H2O 0.74
Tris Base 1.21
Available Package Sizes
Na2EDTA2H2O 0.37
100g 500g
Available Package Sizes
T774 - Tris Borate EDTA Buffer Solution (10x) 100g 500g
TBE Buffer
Sterile Filtered T752 - Tris EDTA Buffer Solution (10X), pH 8.0
DNAse/RNAse Free TE Buffer
Liquid Sterile Filtered
Miscible with Water DNAse/RNAse Free
Store at Room Temperature Liquid
Miscible with Water
Components (g/L)
Store at Room Temperature
Tris Base 107.8
Components (g/L)
Boric Acid 55.0
Tris Base 1.21
Na2EDTA2H2O 7.4
Na2EDTA2H2O 0.37
Available Package Sizes
Contains sufficient HCl to adjust pH to 8.0
500mL 1L
Available Package Sizes
500mL 1L

Page 148 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
T814 - Tris-Glycine Buffer T858 - Tris Hydrochloride
TG Buffer 2-Amino-2-hydroxymethyl-1,3-propanediol Hydrochloride
Use 17.44 grams to make 1 liter of 1x buffer solution Powder
Powder CAS Number: 1185-53-1
Soluble in Water Formula: C4H11NO3HCl
Store at Room Temperature Molecular Weight: 157.6
Components (g/L) Soluble in Water
Store at Room Temperature
Tris Base 3.03
Glycine 14.41
Available Package Sizes
100g 500g 1Kg
Available Package Sizes
100g 500g T8100 - Triton X-100
Octoxynol; X-100; 4-(1,1,3,3-Tetramethylbutyl)phenyl-
T821 - Tris Glycine SDS Buffer polyethylene glycol; t-Octylphenoxypolyethoxyethanol;
TG-SDS Buffer / TGS Buffer Polyethylene glycol tert-octylphenyl ether
Use 18.44 grams to make 1 liter of 1x buffer solution Triton X-100 is a nonionic surfactant. Its often used to
Powder solubilized proteins in molecular biology applications.
Soluble in Water Liquid
Store at Room Temperature

Molecular Biology
CAS Number: 9002-93-1
Components (g/L) Formula: C14H22O(C2H4O)n (n = 9 to 10)
Molecular Weight: Average 625
Tris Base 3.03 Miscible with Water
Glycine 14.41 Store at Room Temperature
Sodium Dodecyl Sulfate 1.00 Available Package Sizes
Available Package Sizes 10mL 25mL 100mL
100g 500g
V870 - Vancomycin Hydrochloride
T749 - Tris-Glycine-SDS Buffer Solution (10x) Plant Tissue Culture Tested
Protein Running Buffer; TG-SDS Buffer Powder
Sterile Filtered CAS Number: 1404-93-9
DNAse Free Formula: C66H75Cl2N9O24HCl
Liquid Molecular Weight: 1485.73
Miscible with Water Soluble in Water
Store at Room Temperature Store at 2 to 6 C
Merck 13, 9995
Components (g/L)
Tris Base 30.28
Available Package Sizes
1g 5g 10g 25g
Glycine 144.14
Sodium Dodecyl Sulfate 10.0 V8370 - Vancomycin Hydrochloride Solution
Available Package Sizes (100 mg/mL)
500mL 1L Aqueous Solution
Sterile Filtered
T764 - Tris HCl Solution 1.0 M, pH 7.5 Plant Tissue Culture Tested
Sterile Filtered Liquid
CAS Number: 1404-93-9
Liquid Formula: C66H75Cl2N9O24HCl
Miscible with Water Molecular Weight: 1485.73
Store at Room Temperature Miscible with Water
Store at -20 to 0 C
Components (g/L)
Ships on dry ice
Tris Base 121.14
Contains sufficient HCl to adjust pH to 7.5
Available Package Sizes Available Package Sizes
500mL 1L 10x1mL 10mL 25mL 100mL

Molecular Biology A-Z Page 149


Product Catalogue & Laboratory Guide
W783 - Water, Plant Tissue Culture Grade X8451 - X-Gluc Stock Solution (20 mg/mL)
Sterile Filtered A solution of X-Gluc (X877) at 20 milligrams per milliliter in
Plant Tissue Culture Tested DMSO (D241).
DNAse/RNAse Free 5-bromo-4-chloro-3-indolyl--D-glucuronic Acid
Liquid Monocyclohexyl Ammonium Salt
CAS Number: 7732-18-5 Liquid
Formula: H2O CAS Number: 114162-64-0
Molecular Weight: 18.02 Formula: C20H26BrClN2O7
Store at Room Temperature Molecular Weight: 521.8
Merck 13, 10098 Miscible with Water
Store at -20C
Available Package Sizes
Light Sensitive
1L 3.9L
Available Package Sizes
W887 - Wilikins-Chalgren Agar 5mL 25mL
See Microbiology Section for Complete Listing
Y889 - YEP Medium
X874 - X-Gal See Microbiology Section for Complete Listing
5-Bromo-4-chloro-3-indolyl--D-galactopyranoside
Y893 - YMB Medium
Molecular Biology

Powder
CAS Number: 7240-90-6 See Microbiology Section for Complete Listing
Formula: C14H15BrClNO6
Molecular Weight: 408.63
Soluble in DMSO or KOH
Store at -20 to 0 C
Available Package Sizes
100mg 500mg 1g

How to Make a GUS Gene Staining Solution


See "Figure 31. GUS Gene Staining Solution Formulation" on
page 260 in the Technical Section.

X877 - X-Gluc, Monocyclohexyl Ammonium Salt


X-GlcA; 5-Bromo-4-chloro-3-indolyl--D-glucuronic Acid,
Monocyclohexyl Ammonium Salt
Powder
CAS Number: 114162-64-0
Formula: C20H26BrClN2O7
Molecular Weight: 521.8
Soluble in DMSO or KOH
Store at -20 to 0 C
Available Package Sizes Custom Media & Services
100mg 500mg 1g 5g PhytoTechnology Laboratories
offers custom manufacturing
X871 - X-Gluc, Sodium Salt and testing services. For more
5-Bromo-4-chloro-3-indolyl--D-glucuronic Acid, Sodium Salt information please see page
12.
Powder
CAS Number: 129541-41-9 Custom Dry Powder Media
Formula: C14H12BrClNaNO7
Molecular Weight: 444.6 Custom Liquid Media
Soluble in DMSO or KOH
Store at -20 to 0 C Custom Packaging
Available Package Sizes Testing Services
100mg 500mg 1g 5g

Page 150 2015 PhytoTechnology Laboratories


Product Catalogue & Laboratory Guide
Microbiology Czapex-Dox Broth & Agar
PhytoTechnology Laboratories offers a line of See Phytopathology Section for Complete Listing
media for the growth of bacteria and fungi. These H289 - Hanahans Broth
media are manufactured with the same dedication SOB Medium was developed by Hanahan (1983) for the
and standards of quality that has made the cultivation of competent E. coli cells prior to transformation.
PhytoTechnology Laboratories plant tissue culture This is a nutritionally rich medium to which 22 mM (0.4%)
media so popular. D-glucose is added for the final stage of transformation; this
allows the E. coli cells to mend from transformation and
replicate.
Most components used in the formulation of
Store at 2 to 6 C
Microbiology Media are available as individual Soluble in Water
components. Check the Biochemicals Section on Use at 28.09 grams per liter
Page 15 for component product listings and more Components (mg/L)
information. Tryptone 20,000
Yeast Extract 5000
Be sure to check www.phytotechlab.com frequently
Magnesium Sulfate, Anhydrous 2400
as we continue to expand this product line.
Sodium Chloride 500
Potassium Chloride 186
For More Information on Microbiology Media Approximate pH at Room Temperature 7.0 0.5
See "Phytopathology & Microbiology Media" on page 261 in
Microbiology

the Technical Section.


Available Package Sizes
1L 100g 500g
A1272 - Aniline Blue
See Seed Testing Section for Complete Listing
K5013 - Kings B Medium
See Seed Testing Section for Complete Listing
B129 - Bacteria Screening Medium 523
See Phytopathology Section for Complete Listing
L5128 - LB Agar, Lennox L Modification
Contains the nutrients as described by Bertani (1951), and
B1714 - Bromothymol Blue Luria & Burrows (1955). Formulation modified by Lennox L
See Seed Testing Section for Complete Listing (1955).
Also known as Lysogeny Agar or Luria-Bertani Agar
C184 - Casein, Enzymatic Hydrolysate Store at 2 to 6 C
See Biochemicals Section for Complete Listing Soluble in Water (Partially)
Use at 32.00 grams per liter
C1842 - Cellobiose Components (mg/L)
See Seed Testing Section for Complete Listing Sodium Chloride 5,000
C1970 - Cephalexin Monohydrate Agar 12,000
See Seed Testing Section for Complete Listing Tryptone 10,000
Yeast Extract 5,000
C2112 - Cephalexin Solution (100 mg/mL) Approximate pH at Room Temperature 6.9 0.5
See Seed Testing Section for Complete Listing
Available Package Sizes
C442 - Corn Meal Agar 1L 500g 5Kg
See Phytopathology Section for Complete Listing

C1830 - Crystal Violet


See Seed Testing Section for Complete Listing

H276 - CTAB
See Molecular Biology Section for Complete Listing

H3818 - CTAB Solution (100 mg/mL)


See Molecular Biology Section for Complete Listing

Page 152 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
L465 - LB Agar, Miller Modification L475 - LB Broth, Miller Modification
Contains the nutrients as described by Bertani (1951), and Contains the nutrients as described by Bertani (1951), and
Luria & Burrows (1955). Formulation modified by Miller Luria & Burrows (1955). Formulation modified by Miller
(1972). (1972).
This is the most widely-used formulation of LB Broth, This is the most widely-used formulation of LB Broth,
Stambrook (1989). Stambrook (1989).
Also known as Lysogeny Agar or Luria-Bertani Agar Also known as Lysogeny Broth or Luria-Bertani Broth
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water Soluble in Water
Use at 37.00 grams per liter Use at 25.00 grams per liter
Components (mg/L) Components (mg/L)
Agar 12,000 Sodium Chloride 10,000
Sodium Chloride 10,000 Tryptone 10,000
Tryptone 10,000 Yeast Extract 5,000
Yeast Extract 5,000 Approximate pH at Room Temperature 7.0 0.5
Approximate pH at Room Temperature 7.0 0.5 Available Package Sizes
Available Package Sizes 1L 500g 5Kg
1L 500g 5Kg
L585 - LB Broth Solution, Lennox L Modification
L5138 - LB Broth, Lennox L Modification (1x)

Microbiology
Contains the nutrients as described by Bertani (1951), and Contains the nutrients as described by Bertani (1951), and
Luria & Burrows (1955). Formulation modified by Lennox L Luria & Burrows (1955). Formulation modified by Lennox L
(1955). (1955).
Also known as Lysogeny Broth or Luria-Bertani Broth Also known as Lysogeny Broth or Luria-Bertani Broth
Store at 2 to 6 C Liquid
Soluble in Water Store at 2 to 6 C
Use at 20.00 grams per liter Sterile Filtered
Components (mg/L) Miscible with water
Sodium Chloride 5,000 Components (mg/L)
Tryptone 10,000 Sodium Chloride 5,000
Yeast Extract 5,000 Tryptone 10,000
Approximate pH at Room Temperature 6.75 0.75 Yeast Extract 5,000
Approximate pH at Room Temperature 6.9 0.5
Available Package Sizes
1L 500g 5Kg Available Package Sizes
100mL 500mL 1L
L5190 - LB Broth, Lennox L Modification (ADP Free)
Contains the nutrients as described by Bertani (1951), and
Luria & Burrows (1955). Formulation modified by Lennox L
(1955). Also known as Lysogeny Broth or Luria-Bertani Broth
Store at 2 to 6 C
Soluble in Water
Free of Animal Derived Products (ADP)
Use at 24.0 grams per liter
Components (mg/L)
Peptone, from Soy 14,000
Yeast Extract 5000
Sodium Chloride 5000
Approximate pH at Room Temperature 6.75 0.75
Available Package Sizes
1L 500g

Microbiology A-Z Page 153


Product Catalogue & Laboratory Guide
L301 - LB Broth Solution, Miller Modification (1x) M498 - Malt Extract Agar
Contains the nutrients as described by Bertani (1951), and Store at Room Temperature
Luria & Burrows (1955). Formulation modified by Miller Soluble in Water (Partially)
(1972). Use at 48.00 grams per liter
This is the most widely-used formulation of LB Broth, Components (mg/L)
Stambrook (1989).
Agar 15,000
Also known as Lysogeny Broth or Luria-Bertani Broth
Malt Extract 30,000
Liquid
Store at 2 to 6 C Peptone from Meat 3,000
Sterile Filtered Approximate pH at Room Temperature 6.4 0.5
Miscible with water
Available Package Sizes
Components (mg/L)
500g 1Kg
Tryptone 10,000
Yeast Extract 5000 M5506 - Malt Extract Agar with Glucose
Sodium Chloride 10,000 and Maltose
Malt Extract Agar is a nutrient medium used for the isolation,
Available Package Sizes
detection, transformation and growth of yeasts and fungi in
100mL 500mL 1L microbiology applications. Formulation as published by Thom
& Church (1926).
L591 - LB Broth Solution with Glycerol
Store at 2 to 6 C
Contains the nutrients as described by Bertani (1951), and
Soluble in Water
Luria & Burrows (1955). Formulation modified by Lennox L
Microbiology

Use at 30.0 grams per liter of medium


(1955).
Also known as Lysogeny Broth or Luria-Bertani Broth Components (mg/L)
Liquid Agar 15,000
Store at 2 to 6 C D-Glucose, anhydrous 6000
Sterile Filtered Malt Extract 6000
Miscible with water
Maltose 1800
Components (mg/L)
Yeast Extract 1200
Glycerol 4.0 mL
Sodium Chloride 5,000
Available Package Sizes
1L 100g 500g
Tryptone 10,000
Yeast Extract 5,000 M484 - Malt Extract Broth
Approximate pH at Room Temperature 6.9 0.5 Store at Room Temperature
Available Package Sizes Soluble in Water
Use at 20.00 grams per liter
100mL 500mL 1L
Components (mg/L)
L476 - Leifert & Waites Sterility Test Medium Malt Extract 17,000
See Phytopathology Section for Complete Listing Peptone from Meat 3,000
Approximate pH at Room Temperature 6.5 0.5
M474 - Malt Extract
See Biochemicals Section for Complete Listing Available Package Sizes
1L 500g 1Kg

M5516 - mD5A Medium


See Seed Testing Section for Complete Listing

M567 - Meat Extract


See Biochemicals Section for Complete Listing

M5781 - Methylene Blue


See Seed Testing Section for Complete Listing

Page 154 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
M580 - Modified Melin-Norkrans Medium P6737 - PCNB
Used for the isolation of Fungi from mixed microbiological See Seed Testing Section for Complete Listing
samples.
Store at 2 to 6 C P777 - Penicillin G
Soluble in Water (Partially) See Biochemical Section for Complete Listing
Use at 20.82 grams per liter
P6767 - Penicillin G Solution (10 mg/mL)
Components (mg/L)
See Seed Testing Section for Complete Listing
Ammonium Phosphate, Monobasic 184.7
Calcium Chloride, Anhydrous 50 P721 - Peptone, from Soymeal
FeNaEDTA 20 See Biochemicals Section for Complete Listing
Magnesium Sulfate 35.8
P780 - Peptone, Glysate
Potassium Phosphate, Monobasic, Anhydrous 500
See Biochemicals Section for Complete Listing
Sodium Chloride 25
Agar 7,000 P775 - Peptone, Type I
Malt Extract 3,000 See Biochemicals Section for Complete Listing
Sucrose 10,000
P632 - Peptone Water (1x)
ThiamineHCl 0.1
Approximate pH at Room Temperature 6.5 0.5 Liquid
Store at 2 to 6 C
Available Package Sizes Miscible with Water

Microbiology
1L 10L Sterile Filtered
Components (mg/L)
Indexing with Microbiology Media
Peptone from Meat 10,000
See "Figure 20. Media Used in Indexing Explants" on page
248 in the Technical Section. Sodium Chloride 5,000
Approximate pH at Room Temperature 7.2 0.5
N601 - Nutrient Agar
Available Package Sizes
Store at Room Temperature
100mL 500mL
Soluble in Water (Partially)
Use at 23.00 grams per liter P732 - Peptone Water, Buffered (1x)
Components (mg/L)
Liquid
Agar 15,000 Store at 2 to 6 C
Meat Extract 3,000 Miscible with Water
Peptone from Meat 5,000 Sterile Filtered
Approximate pH at Room Temperature 7.0 0.5
Available Package Sizes Components (mg/L)
1L 100g 500g Peptone from Meat 10,000
Sodium Chloride 5000
N611 - Nutrient Broth Sodium Phosphate, Dibasic 3500
Store at Room Temperature Potassium Phosphate, Monobasic 1500
Soluble in Water
Approximate pH at Room Temperature 7.2 0.5
Use at 8.00 grams per liter
Components (mg/L) Available Package Sizes
Meat Extract 3,000 100mL 500mL 1L
Peptone from Meat 5,000 P6800 - PhytoSelect Basal Medium
Approximate pH at Room Temperature 7.0 0.5 See Seed Testing Section for Complete Listing
Available Package Sizes
P6809 - Polymyxin B Sulfate
1L 500g
See Seed Testing Section for Complete Listing
O606 - Oat Meal Agar
P6719 - Polymyxin B Solution (10 mg/mL)
See Phytopathology Section for Complete Listing
See Seed Testing Section for Complete Listing

Microbiology A-Z Page 155


Product Catalogue & Laboratory Guide
Potato Dextrose Broth & Agar S657 - SOC Medium Solution
See Phytopathology Section for Complete Listing Typically used in the recovery step of Escherichia coli competent
cell transformations. Formulation as described by Hanahan
New Product (1983).
P6750 - Potato Infusion Powder Liquid
Potato Extract; Dehydrated Infusion of Potato Store at 2 to 6 C
A supplement added to microbiological media to encourage Sterile Filtered
growth of bacteria and fungi. Miscible with water
Powder Components (mg/L)
Soluble in Water Glucose, Anhydrous 3,600
Store at Room Temperature Magnesium Sulfate, Anhydrous 2,400
Available Package Sizes Potassium Chloride 186
500g 2.5Kg Sodium Chloride 500
Tryptone 20,000
P692 - Potato Powder
Yeast Extract 5,000
See Biochemicals Section for Complete Listing
Approximate pH at Room Temperature 7.2 0.5
R7171 - Rose Bengal Available Package Sizes
See Seed Testing Section for Complete Listing
100mL 500mL 1L
S7536 - Sabouraud Dextrose Broth S7478 - Sordaria Crossing Agar
Microbiology

Used in the isolation and cultivation of yeasts, fungi.


Used as medium for genetic crossover culture of the fungus
Store at Room Temperature Sordaria fimicola.
Soluble in Water
Store at 2 to 6 C
Use at 50.0 grams per liter
Soluble in Boiling Water
Components (mg/L) Use at 35.1 grams per liter
D-(+) Glucose, Anhydrous 40,000 Components (mg/L)
Peptone from Meat 10,000 Agar 15,000
Available Package Sizes Corn Meal 2000
1L 100g 500g D-Glucose, Anhydrous 7000
Potassium Phosphate, Monobasic 100
S7775 - Saline Solution (0.85%) Sucrose 10,000
See Seed Testing Section for Complete Listing
Yeast Extract 1000
Available Package Sizes
1L 100g 500g 5Kg

T8199 - Tetrazolium Blue


See Biochemicals Section for Complete Listing

Page 156 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
T760 - Terrific Broth T7900 - Tryptic Soy Broth
Terrific Broth is a nutrient media for use with the culture Tryptic Soy Broth, Type I
Escherichia coli for microbiology applications. Store at Room Temperature
Requires the addition of 4.0 mL of Glycerol (Product Number Soluble in Water
G381) per liter of broth. Use at 25.0 grams per liter of medium
Powder
Components (mg/L)
Store at 2 to 6 C
Soluble in Water Tryptone 15,000
Use at 47.00 grams per liter Peptone from Soymeal 5000
Components (mg/L) Sodium Chloride 5000
Tryptone 11,800 Approximate pH at Room Temperature 7.0 0.5
Potassium Phosphate, Dibasic, Anhydrous 9,400 Available Package Sizes
Potassium Phosphate, Monobasic, Anhydrous 2,200 1L 500g 1Kg
Yeast Extract 23,600
T8000 - Tryptic Soy Broth, Type II
Approximate pH at Room Temperature 7.3 0.5
Store at Room Temperature
Available Package Sizes Soluble in Water
1L 500g 5kg Use at 30.0 grams per liter of medium

T754 - Terrific Broth Solution with Glycerol (1x) Components (mg/L)


Terrific Broth is a nutrient media for use with the culture Tryptone 17,000

Microbiology
Escherichia coli for microbiology applications. Peptone from Soymeal 3000
Liquid Sodium Chloride 5000
Store at 2 to 6 C Potassium Phosphate, Dibasic 2500
Sterile Filtered D- Glucose 2500
Miscible with water
Components (mg/L)
Available Package Sizes
1L 500g 1Kg
Glycerol 4.0 mL
Tryptone 11,800 T832 - Tryptone
Potassium Phosphate, Dibasic, Anhydrous 9400 See Biochemicals Section for Complete Listing
Potassium Phosphate, Monobasic, Anhydrous 2200
T848 - Tryptose
Yeast Extract 23,600
See Biochemicals Section for Complete Listing
Approximate pH at Room Temperature 7.3 0.5
Available Package Sizes T8110 - Tyrothricin
100mL 500mL 1L See Seed Testing Section for Complete Listing

T7968 - Trehalose Dihydrate T8020 - Tyrothricin (10 mg/mL)


See Seed Testing Section for Complete Listing See Seed Testing Section for Complete Listing

T752 - TRIS EDTA Buffer Solution, pH 8.0 W783 - Water, Plant Tissue Culture Grade
See Molecular Biology Section for Complete Listing See Molecular Biology Section for Complete Listing

Microbiology A-Z Page 157


Product Catalogue & Laboratory Guide
W887 - Wilkins-Chalgren Agar Y8488 - Yeast Malt Broth
This medium is typically used to isolate and culture anaerobic Also known as Yeast Mold Broth.
bacteria. Formulation as described by Wilkens & Chalgren Store at 2 to 6 C
(1976). Soluble in Water
Store at 2 to 6 C Use at 21.0 grams per liter
Prepared medium should be stored at 2 to 6 C in the dark.
Components (mg/L)
Soluble in Water (Partially)
Use at 43.00 grams per liter Peptone from Meat 5000
Components (mg/L) Yeast Extract 3000
Agar 10,000 D- Glucose, Anhydrous 10,000
L-Arginine (Free Base) 1,000 Malt Extract 3000
D-Glucose 1,000 Available Package Sizes
Haemin 5.0 1L 500g
Menadione 0.5
New Product
Peptone, Glysate 10,000
Y8935 - Yeast Mannitol Agar
Pyruvic Acid 1,000
Also known as YMA; Yeast Extract Mannitol Agar ; YEMA;
Sodium Chloride 5,000
YEM Agar; YM Agar
Tryptone 10,000
Yeast Extract 5,000 Used for the cultivation, isolation and enumeration of
Approximate pH at Room Temperature 6.9 0.5 Rhizobium spp. and other soil microorganisms.
Microbiology

Available Package Sizes Store at 2 to 6 C


Soluble in Water
500g Use at 27.7 grams per liter
X8454 - XTS Medium Components (mg/L)
See Seed Testing Section for Complete Listing Magnesium Sulfate, Anhydrous 98
Potassium Phosphate, Dibasic 500
Y892 - Yeast Extract
Sodium Chloride 100
See Biochemicals Section for Complete Listing
Agar 15,000
New Product D-Mannitol 10,000
Y8575 - Yeast Extract Beef Broth Yeast Extract 1000
YEB Broth Calcium Carbonate 1000
pH at Room Temperature 3.5 0.75
This medium has been used extensively to culture Agrobacterium
Available Package Sizes
species.
500g 1Kg
Store at 2 to 6 C
Soluble in Water
Use at 16.24 grams per liter
Components (mg/L)
Magnesium Sulfate, Anhydrous 240
Meat Extract 5000
Peptone from Meat 5000
D-Sucrose 5000
Yeast Extract 1000
pH at Room Temperature 3.5 0.75
Available Package Sizes
1L 10L 500g

Page 158 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
New Product Y8480 - YEP Medium (ADP Free)
Y8930 - Yeast Mannitol Broth Also known as Yeast Extract Peptone Medium. This has been
used to culture Agrobacterium as well as yeasts.
Also known as YM Broth; Yeast Extract Mannitol Broth; YEM
Broth; YEMB Store at 2 to 6 C
Soluble in Boiling Water
Used for the cultivation, isolation and enumeration of Free of Animal Derived Products (ADP)
Rhizobium spp. and other soil microorganisms. Use at 45.0 grams per liter
Store at 2 to 6 C Components (mg/L)
Soluble in Water Peptone from Soy 15,000
Use at 12.7 grams per liter Yeast Extract 10,000
Components (mg/L) Sodium Chloride 5,000
Magnesium Sulfate, Anhydrous 98 Agar 15,000
Potassium Phosphate, Dibasic 500 Available Package Sizes
Sodium Chloride 100 1L 500g
D-Mannitol 10,000
Yeast Extract 1000
Calcium Carbonate 1000
pH at Room Temperature 3.5 0.75
Available Package Sizes

Microbiology
500g 1Kg

Y8565 - YEP Broth


Also known as Yeast Extract Peptone Broth. This has been used
to culture Agrobacterium as well as yeasts.
Store at 2 to 6 C
Soluble in Water
Use at 25.0 grams per liter
Components (mg/L)
Peptone from Meat 10,000
Yeast Extract 10,000
Sodium Chloride 5000
Available Package Sizes
1L 10L 500g

Y889 - YEP Medium


Also known as Yeast Extract Peptone Medium. This has been
used to culture Agrobacterium as well as yeasts.
Store at 2 to 6 C
Soluble in Water (Partially)
Use at 40.00 grams per liter
Components (mg/L)
Agar 15,000
Peptone from Meat 10,000
Sodium Chloride 5,000
Yeast Extract 10,000
Approximate pH at Room Temperature 6.6 0.5
Available Package Sizes
1L 500g

Microbiology A-Z Page 159


Product Catalogue & Laboratory Guide
Introducing the PhytoTechnology Laboratories Designed, Ergonomic
Glass Bead Sterilizer: S7510/S7520 ErgoSteri VT
ErgoSteri VT
PhytoTechnology Laboratories is pleased to introduce their new
ErgoSteri glass bead sterilizer. This unique unit features a slanted
bead well that is more ergonomically friendly than convention units
that are vertical. Another important feature is a digital temperature
controller that is adjustable between 110 and 350 C. Most units
on the market today feature either factory preset temperatures that
cannot be changed, or analog knobs that make it difficult to set an
exact temperature. The only other digital model, the Steripot brand,
has a very limited temp range (250 to 275 C).

The ErgoSteri was designed with quality and accurate temperature


maintenance in mind. The unit is manufactured and assembled in
the USA, and the digital temperature controller allows for accurate
temperature adjustments within a broad range. The construction
of the unit allows for easy maintenance and care-free operation;
however, PhytoTech offers a one-year warranty on parts & labor
should a problem be encountered. And since all manufacturing and
assembly are performed in the USA PhytoTech can offer a quick
turnaround if any unit should require warranty work.

The ErgoSteri heats fast, reading a stable 200 C in under 20


minutes. PhytoTechs unique design also prohibits glass beads from
sliding into any other part of the unit other than the bead well
making filling and emptying much easier.

Please visit our website for more information on the ErgoSteri glass
bead sterilizer.
Product Pkg
Product Description Product Notes Dimensions and Other Info
Number Size
20.1 cm
Temperature Read-Out: Digital Body Height:
(8.25 in)
ERGOSTERI VT, 110 V
S7510 14.5 cm
Glass Bead Sterilizer Body Width:
110 to 350C (5.75 in)
Temperature Range:
Body Length: 23.5 cm
1 ea
(front to back) (9.25 in)
Manufactured in: USA 5 cm
ERGOSTERI VT, 220 V Bead Well Diameter:
S7520 (2 in)
Glass Bead Sterilizer
Warranty: 1-year 12.7 cm
Bead Well Depth:
(5 in)
BEAD REPLACEMENTS Packaged specifically for the ErgoSteri
S638 Quantity: 450 g 1 ea
Glass Bead Sterilizer Beads bead well capacity.

Temperature Ergonomic
Competitor Model Country of Origin Temperature
Read-Out Design
A MODEL BS-1000 INDIA PRE-SET NONE NO

B STERI-350 SWITZERLAND PRE-SET ANALOG NO

C STERIPOT-S150D with Digital Controller INDIA ADJUSTABLE DIGITAL NO

THE
D LAB ASSOCIATES G3301
NETHERLANDS
ADJUSTABLE NONE NO

PhytoTech ERGOSTERI VT USA ADJUSTABLE DIGITAL YES

Page 160 2015 PhytoTechnology Laboratories


Product Catalogue & Laboratory Guide
Phytopathology C1842 - Cellobiose
PhytoTechnology Laboratories offers a line of media See Seed Testing Section for Complete Listing
and products for use in phytopathology research. C1970 - Cephalexin Monohydrate
Phytopathology media are manufactured with the See Seed Testing Section for Complete Listing
same dedication and standards of quality that has
made PhytoTechnology Laboratories plant tissue C2112 - Cephalexin Solution (100 mg/mL)
culture media so popular. See Seed Testing Section for Complete Listing

C442 - Corn Meal Agar


Most components used in the formulation of
Store at Room Temperature
Phytopathology Media are available as individual Soluble in Water (Partially)
components. Check the Biochemicals Section on Use at 22.00 grams per liter
Page 15 for component product listings and more Components (mg/L)
information. Agar 7,000
Corn Meal 15,000
Be sure to check www.phytotechlab.com frequently
Approximate pH at Room Temperature 7.2 0.5
as we continue to expand this product line.
Available Package Sizes
1L 100g 500g
For More Information on Phytopathology Media
Phytopathology

See "Phytopathology & Microbiology Media" on page 261 in C1830 - Crystal Violet
the Technical Section. See Seed Testing Section for Complete Listing
A1286 - Alcian Blue H276 - CTAB
See Seed Testing Section for Complete Listing See Molecular Biology Section for Complete Listing
A1272 - Aniline Blue H3818 - CTAB Solution (100 mg/mL)
See Seed Testing Section for Complete Listing See Molecular Biology Section for Complete Listing
B129 - Bacteria Screening Medium 523 C506 - CzapekDox Agar
Used to identify pathogen-free explants. Does not promote Czapek-Dox broth is a semi-synthetic broth used for the
explant growth. Formulation as described by Viss & Driver cultivation of fungi. This medium is prepared according to the
(1991). formula developed by Thom and Church (1926).
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water (Partially) Soluble in Water (Partially)
Use at 32.15 grams per liter Use at 50.01 grams per liter
Plant Tissue Culture Tested
Components (mg/L)
Components (mg/L)
Agar 15,000
Magnesium Sulfate7H2O 150
Ferrous Sulfate7H2O 10
Potassium Phosphate, Monobasic, Anhydrous 2,000
Magnesium Sulfate, Anhydrous 500
Agar 8,000
Potassium Chloride 500
Casein, Enzymatic Hydrolysate 8,000
Potassium Phosphate, Dibasic, Anhydrous 1,000
Sucrose 10,000
Sodium Nitrate 3,000
Yeast Extract 4,000
Sucrose 30,000
Approximate pH at Room Temperature 6.0 0.5
Approximate pH at Room Temperature 7.9 0.5
Available Package Sizes
Available Package Sizes
1L 100g 500g 1Kg
1L 500g
B1714 - Bromothymol Blue
See Seed Testing Section for Complete Listing

C184 - Casein, Enzymatic Hydrolysate


See Biochemicals Section for Complete Listing

Page 162 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
C443 - CzapekDox Broth L476 - Leifert & Waites Sterility Test Medium
Czapek-Dox broth is a semi-synthetic broth used for the This medium was developed to determine and identify potential
cultivation of fungi. This medium is prepared according to the pathogens in explants. Formulation as described by Leifert, C
formula developed by Thom and Church (1926). & WM Waites (1992).
Store at 2 to 6 C Store at 2 to 6 C
Soluble in Water Soluble in Water
Use at 35.01 grams per liter Use at 45.22 grams per liter
Components (mg/L) Plant Tissue Culture Tested
Ferrous Sulfate7H2O 10 Components (mg/L)
Magnesium Sulfate, Anhydrous 500 Ammonium Nitrate 825
Potassium Chloride 500 Boric Acid 3.1
Potassium Phosphate, Dibasic, Anhydrous 1,000 Calcium Chloride, Anhydrous 166.1
Sodium Nitrate 3,000 Cobalt Chloride6H2O 0.0125
Sucrose 30,000 Cupric Sulfate5H2O 0.0125
Approximate pH at Room Temperature 8.3 0.5 Na2EDTA2H2O 18.63
Ferrous Sulfate7H2O 13.9
Available Package Sizes
Magnesium Sulfate, Anhydrous 90.35
1L 500g
Manganese SulfateH2O 8.45
G3574 - Guaiacol Molybdic Acid, Disodium Salt2H2O 0.125

Phytopathology
See Seed Testing Section for Complete Listing Potassium Iodide 0.415
Potassium Nitrate 950
Indexing with Phytopathology Media
Potassium Phosphate, Monobasic, Anhydrous 85
See "Figure 20. Media Used in Indexing Explants" on page
248 in the Technical Section. Sodium Chloride 2,000
Zinc Sulfate7H2O 4.3
D-Glucose, Anhydrous 5,000
Glycine 1.0
myo-Inositol 50
Meat Extract 7,000
Nicotinic Acid 0.25
Molecular Biology Kits Peptone from Meat 4,000
PhytoTechnology Laboratories offers a variety PyridoxineHCl 0.25
of kits for molecular biology. Please see the Sucrose 15,000
molecular biology section on pages 131 to ThiamineHCl 0.05
150 for more information. Yeast Extract 10,000
RNA Extraction Kits Approximate pH at Room Temperature 6.8 0.5
Available Package Sizes
Genomic DNA Extraction Kits
500g 1Kg
Plasmid DNA Extraction Kits
M474 - Malt Extract
DNA Ladder Kits See Biochemicals Section for Complete Listing

M567 - Meat Extract


See Biochemicals Section for Complete Listing

M5781 - Methylene Blue


See Seed Testing Section for Complete Listing

Phytopathology A-Z Page 163


Product Catalogue & Laboratory Guide
O606 - Oat Meal Agar P692 - Potato Powder
Store at Room Temperature See Biochemicals Section for Complete Listing
Soluble in Water (Partially)
Use at 10.50 grams per liter R7171 - Rose Bengal
Components (mg/L) See Seed Testing Section for Complete Listing
Agar 7,000 S7775 - Saline Solution (0.85%)
Rolled Oats 3,500 See Seed Testing Section for Complete Listing
Approximate pH at Room Temperature 6.9 0.5
Available Package Sizes T8199 - Tetrazolium Blue
See Biochemicals Section for Complete Listing
1L 100g 500g

P6737 - PCNB T7968 - Trehalose Dihydrate


See Seed Testing Section for Complete Listing
See Seed Testing Section for Complete Listing

P777 - Penicillin G T8092 - Toluidine Blue O


See Seed Testing Section for Complete Listing
See Seed Testing Section for Complete Listing

P6767 - Penicillin G Solution (10 mg/mL) T8110 - Tyrothricin


See Seed Testing Section for Complete Listing
See Seed Testing Section for Complete Listing
T8020 - Tyrothricin (10 mg/mL)
Phytopathology

Peptone From Meat -or- Peptone From Soy


See Seed Testing Section for Complete Listing
See Biochemicals Section for Complete Listings

P6809 - Polymyxin B Sulfate T752 - TRIS EDTA Buffer Solution, pH 8.0


See Molecular Biology Section for Complete Listing
See Seed Testing Section for Complete Listing

P6719 - Polymyxin B Solution (10 mg/mL) T832 - Tryptone


See Biochemicals Section for Complete Listing
See Seed Testing Section for Complete Listing

P772 - Potato Dextrose Agar T848 - Tryptose


See Biochemicals Section for Complete Listing
Store at Room Temperature
Soluble in Water (Partially) W783 - Water, Plant Tissue Culture Grade
Use at 39.00 grams per liter
See Molecular Biology Section for Complete Listing
Components (mg/L)
Agar 15,000 W887 - Wilkins-Chalgren Agar
D-Glucose, Anhydrous 20,000 See Microbiology Section for Complete Listing
Potato Powder 4,000
X8454 - XTS Medium
Approximate pH at Room Temperature 7.0 0.5
See Seed Testing Section for Complete Listing
Available Package Sizes
1L 100g 500g 2.5Kg Y892 - Yeast Extract
See Biochemicals Section for Complete Listing
P762 - Potato Dextrose Broth
Store at Room Temperature Y8488 - Yeast Malt Broth
Soluble in Water See Microbiology Section for Complete Listing
Use at 24.00 grams per liter
Components (mg/L)
YEP Broths & Medium
See Microbiology Section for Complete Listings
D-Glucose, Anhydrous 20,000
Potato Powder 4,000 Y893 - YMB Medium
Approximate pH at Room Temperature 7.0 0.5 See Microbiology Section for Complete Listing
Available Package Sizes
1L 100g 500g

Page 164 2015 PhytoTechnology Laboratories


Product Catalogue & Laboratory Guide
Seed Testing B1714 - Bromothymol Blue
PhytoTechnology Laboratories introduced the Seed Bromothymol Blue Sodium Salt, BTB,
4,4-(1,1-dioxido-3H-2,1-benzoxathiole-3,3-diyl)bis(2-bromo-
Testing product line in late 2012.
6-isopropyl-3-methylphenol)
A biological (histological) stain and pH indicator.
Most components used in the formulation of Seed Yellow at pH 6.0, green at pH 6.7, and blue at pH 7.6.
Testing Media are available as individual components. Powder
Check the Biochemicals Section on Page 15 for CAS Number: 34722-90-2
component product listings and more information. Formula: C27H27Br2O5SNa
Molecular Weight: 646.37
Soluble in Water
Check www.phytotechlab.com frequently as we Store at Room Temperature
continue to expand this product line.
Available Package Sizes
10g 25g
For More Information on Stains & Dyes
See "Figure 35. Stains & Dyes Usage Table" on page 264 in C1933 - Calcofluor White M2R
the Technical Section. See Biochemicals Section for Complete Listing

A1286 - Alcian Blue New Product


Alcian Blue 8GX C1759 - Carmine
A biological stain useful for staining pectins in plant cell walls.
C.I.# 75470; Cochineal; Nacarat; Natural Red 4; Crimson Lake
Powder
Powder
Seed Testing

CAS Number: 75881-23-1


CAS Number: 1390-65-4
Formula: C56H68Cl4CuN16S4
Formula: C22H20O13
Molecular Weight: 1298.86
Molecular Weight: 492.39
Soluble in Water
Soluble in NaOH, KOH (1 mg/mL); Slightly
Store at Room Temperature
Soluble in Hot Water
Available Package Sizes Store at Room Temperature
10g 25g 100g Merck 13, 1855
Available Package Sizes
A1272 - Aniline Blue
5g 25g 100g
A histological stain used to detect fungal contaminants during
seed testing. Sometimes used with Lactic Acid during seed C1842 - Cellobiose
testing.
A general carbon source. Can be used as a carbon source in
Powder mD5A medium for the selection of
CAS Number: 28631-66-5 Xanthomonas hortorum.
Formula: C32H25N3O9S3Na2
Powder
Molecular Weight: 737.72
CAS Number: 528-50-7
Soluble in Water
Formula: C12H22O11
Store at Room Temperature
Molecular Weight: 342.30
Available Package Sizes Soluble in Water
1g 5g 25g 50g Store at Room Temperature
Available Package Sizes
A116 - Ampicillin
10g 25g 100g
See Molecular Biology Section for Complete Listing

A1116 - Ampicillin Solution (100 mg/mL)


See Molecular Biology Section for Complete Listing

B132 - Bacitracin Zinc


See Molecular Biology Section for Complete Listing

Page 166 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
C1970 - Cephalexin Monohydrate H3818 - CTAB Solution (100 mg/mL)
An antibiotic used with XTS Medium (X8454) and PhytoSelect See Molecular Biology Section for Complete Listing
Medium (P6800) for the isolation of Xanthomonas translucens
from Barley and Wheat seeds, Xanthomonas camperstris from C375 - Cupric Sulfate
Brassicas, and other plant pathogens. See Biochemicals Section for Complete Listing
Powder
CAS Number: 23325-78-2 C1989 - Cycloheximide
Formula: C16H17N3O4SH2O See Molecular Biology Section for Complete Listing
Molecular Weight: 365.4
Soluble in Water, DMSO C1796 - Cycloheximide Solution (100 mg/mL)
Store at Room Temperature See Molecular Biology Section for Complete Listing
Available Package Sizes E2799 - Evans Blue
5g 10g 25g
See Biochemicals Section for Complete Listing
C2112 - Cephalexin Solution (100 mg/mL) F3178 - Fast Green (FCF)
A solution of Cephalexin Monohydrate (C1970) at 100
ethyl-[4-[[4-[ethyl-[(3-sulfophenyl)methyl]amino]phenyl]-
milligrams per milliliter of DMSO. An antibiotic used with
(4-hydroxy-2-sulfophenyl)methylidene]-1-cyclohexa-2,5-
XTS Medium (X8454) and PhytoSelect Medium (P6800) for
dienylidene]-[(3-sulfophenyl)methyl]azanium, C.I. 42053
the isolation of Xanthomonas translucens from Barley and Wheat
seeds, Xanthomonas campestris from Brassicas, and other plant Powder
pathogens. CAS Number: 2353-45-9
Formula: C37H34N2O10S3Na2
Liquid
Molecular Weight: 808.85

Seed Testing
CAS Number: 23325-78-2
Soluble in Water
Formula: C16H17N3O4SH2O
Store at Room Temperature
Molecular Weight: 365.4
Miscible withWater Available Package Sizes
Store at -20 to 0 C 5g 10g 25g 100g
Available Package Sizes
G570 - Gentamicin Sulfate
10mL 100mL
See Molecular Biology Section for Complete Listing
C252 - Chloramphenicol
G3350 - Gentamicin Solution (50 mg/mL)
See Molecular Biology Section for Complete Listing
See Molecular Biology Section for Complete Listing
C2010 - Chloramphenicol Solution (10 mg/mL)
G3410 - Gentamicin Solution (100 mg/mL)
See Molecular Biology Section for Complete Listing
See Molecular Biology Section for Complete Listing
C1830 - Crystal Violet
G399 - l-Glutamic Acid
Hexamethylpararosaniline Chloride, Gentian Violet,
See Biochemicals Section for Complete Listing
Basic Violet 3
A biological stain used in PhytoSelect Medium (P6800) for G381 - Glycerol
plant pathogen selection; also used in gram staining, usually
with Safranin O (Product Number S7400). See Biochemicals Section for Complete Listing
Powder G503 - Glycine
CAS Number: 548-62-9
See Biochemicals Section for Complete Listing
Formula: C25H30ClN3
Molecular Weight: 407.98 For More Information about Antibiotics
Soluble in Water
Store at Room Temperature See "Figure 30. Antibiotics/Antimycotics/Selection Agent
Guide" on page 258 in the Technical Section.
Available Package Sizes
25g 100g

H276 - CTAB
See Molecular Biology Section for Complete Listing

Seed Testing A-Z Page 167


Product Catalogue & Laboratory Guide
G3574 - Guaiacol New Product
2-methoxyphenol L5356 - Lactic Acid
Used in peroxidase assays as a colorimetric agent; used in 2-Hydroxypropionic acid; Propanoic acid
cultivar separation of soybeans, and alternatively to detect
H. oryzae in rice seeds. Used in seed testing in place of phenol for seed clearing.
The melting point of this product can be as low as 26.1C, so it can Also used in microbiology/phytopathology media for pH
be either a fine powder or a liquid at room temperature. adjustments. Recommended in ISTA seed testing protocols.
Liquid or Powder Liquid
CAS Number: 90-05-1 CAS Number: 50-21-5
Formula: C7H8O2 Formula: C3H6O3
Molecular Weight: 124.14 Molecular Weight: 90.08
Miscible with and Soluble in Water Miscible with Water
Store at Room Temperature Store at Room Temperature
Available Package Sizes Merck 13, 5351
25g 50g 100g 250g Available Package Sizes
500mL 1L
I4068 - Indigo Carmine
5,5'-Indigodisulfonic Acid Sodium Salt, Indigotine, Acid Blue M562 - d-Mannitol
74, CI#: 73015 See Biochemicals Section for Complete Listing
Used in viability tests to stain seeds to detect dead tissues; stain
will penetrate dead cells, but will not stain living cells. M573 - Malachite Green Oxalate
Can also be used as a pH indicator: it is blue at pH 11.4 and
See Biochemicals Section for Complete Listing
yellow at 13.0.
Seed Testing

Powder M5516 - mD5A Medium


CAS Number: 860-22-0 Modified D-5 Agar Medium.
Formula: C16H8N2Na2O8S2 Used for detecting seedborne Xanthamonas campestris pv. carotae
Molecular Weight: 466.35 on carrot seeds. Recommended in ISTA method 7-020.
Soluble in Water, Ethanol
Store at Room Temperature Store at Room Temperature
Soluble in Boiling Water
Available Package Sizes Use at 20.45 grams per liter of medium
25g 100g Components (mg/L)
K5013 - Kings B Medium Magnesium Sulfate, Heptahydrate 300
Also known as Kings B Agar or KB. Sodium Phosphate, Monohydrate 1150
A non-selective medium for the subculture of suspected isolates. Ammonium Chloride 1000
Glycerol (G381) is often added as a carbon source. Potassium Phosphate, Dibasic 3000
Store at Room Temperature Agar 15,000
Soluble in Boiling Water
Use at 37.23 grams per liter of medium Available Package Sizes
1L 10L
Components (mg/L)
Peptone 20,000 M539 - l-Methionine
Potassium Phosphate, Dibasic 1500 See Biochemicals Section for Complete Listing
Magnesium Sulfate 730
Agar 15,000
Available Package Sizes
1L 500g

Page 168 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
M5781 - Methylene Blue P6767 - Penicillin G Solution (10 mg/mL)
3,7-bis(Dimethylamino)-phenothiazin-5-ium chloride, (2S,5R,6R)-3,3-Dimethyl-7-oxo-6-[(phenylacetyl)amino]-4-
Basic Blue 9, Tetramethylthionine chloride. thia-1-azabicyclo-[3.2.0]heptane-2-carboxylic Acid, Sodium
A biological stain, redox indicator and cationic dye. Salt
Powder An aqueous solution of Penicillin G Sodium Salt (P777) at 10
CAS Number: 7220-79-3 milligrams per milliliter. Used with PhytoSelect Basal Medium
Formula: C16H18ClN3S 3H2O (P6800) for the selection of Xanthomonas campestris.
Molecular Weight: 373.9 Liquid
Soluble in Water CAS Number: 69-57-8
Store at Room Temperature Formula: C16H17N2O4SNa
Available Package Sizes Molecular Weight: 356.4
Miscible with Water
25g 100g Store at -20 to 0 C
Sterile Filtered
N6179 - 1-Naphthol
Ships on Dry Ice
See Biochemicals Section for Complete Listing

N581 - Nystatin Available Package Sizes


See Molecular Biology Section for Complete Listing 10mL 100mL

P6737 - Pentachloronitrobenzene (PCNB) For More Information on the PhytoSelect Basal


Quintozene Medium
An antimycotic used in seed testing, specifically for the See "Seed Testing P6800 PhytoSelect Basal Medium &

Seed Testing
detection of Fusarium circinatum. SMART Media" on page 263 in the Technical Section.
Powder
CAS Number: 82-68-8 P6800 - PhytoSelect Basal Medium
Formula: C6Cl5NO2 PhytoSelect Basal Medium is formulated based on the SMART
Molecular Weight: 295.33 media system developed by Kawanishi et al. (2011)
Soluble in DMSO It requires the addition of antibiotics and a specific carbon
Store at Room Temperature source to be a complete selection medium. See the Product
Available Package Sizes Information Sheet available online for more information.

25g 100g Store at Room Temperature


Soluble in Boiling Water
P777 - Penicillin G, Sodium Salt Use at 24.93 grams per liter of medium
(2S,5R,6R)-3,3-Dimethyl-7-oxo-6-[(phenylacetyl)amino]-4- Components (mg/L)
thia-1-azabicyclo-[3.2.0]heptane-2-carboxylic Acid, Sodium Sodium Phosphate, Monohydrate 5665
Salt
Potassium Phosphate, Monobasic, Anhydrous 3000
Powder Ammonium Chloride 1000
CAS Number: 69-57-8
Formula: C16H17N2O4SNa Magnesium Sulfate, Heptahydrate 250
Molecular Weight: 356.4 Ferrous Sulfate, Heptahydrate 9
Soluble in Water Agar 15,000
Store at Room Temperature
Crystal Violet 3
Merck 13, 7165
Available Package Sizes
Available Package Sizes
1L 5L 10L 50L
25g 100g

Seed Testing A-Z Page 169


Product Catalogue & Laboratory Guide
P6809 - Polymyxin B Sulfate R7171 - Rose Bengal
An antibiotic used with PhytoSelect Medium (P6800) for the 4,5,6,7-tetrachloro-2,4,5,7-tetraiodofluorescein disodium
selection of plant pathogens. salt, Acid Red 94, Bengal Rose B sodium salt, Rose Bengal
Powder sodium salt
CAS Number: 1405-20-5 Powder
Formula: C55H96N16O13 2H2SO4 CAS Number: 632-69-9
Molecular Weight: 1385.61 Formula: C20H2Cl4I4Na2O5
Soluble in Water Molecular Weight: 1017.64
Store at 2 to 6 C Soluble in Water
Store at Room Temperature
Available Package Sizes
100mg 500mg 1g Available Package Sizes
5g 25g
P6719 - Polymyxin B Solution (10 mg/mL)
An aqueous solution of Polymyxin B Sulfate (P6809)at 10 R7278 - Ruthenium Red
milligrams per milliliter. An antibiotic used with PhytoSelect Ammoniated Ruthenium Oxychloride, CI# 77800
Medium (P6800) for the selection of plant pathogens. Ruthenium Red is used to stain pectins, mucilages and gums
Liquid within plant material. Can be used to stain seed
Sterile Filtered mucilage when using a concentration of 0.01% solution.
CAS Number: 1405-20-5 Powder
Formula: C55H96N16O13 2H2SO4 CAS Number: 11103-72-3
Molecular Weight: 1385.61 Formula: H42Cl6N14O2Ru3
Miscible with Water Molecular Weight: 786.35
Seed Testing

Store at -20 to 0 C Soluble in Water


Store at Room Temperature
Available Package Sizes
10mL 100mL Available Package Sizes
100mg 1g 5g 25g
P672 - Potassium Hydroxide
See Biochemicals Section for Complete Listing S7775 - Saline Solution (0.85%)
Used for the extraction of plant pathogens from tissue; can be
P682 - Potassium Hydroxide Solution 1.0 N diluted and plated for pathogen identification.
See Biochemicals Section for Complete Listing Recommended for ISTA methods 7-019, 7-020, and 7-021.
Store at Room Temperature
P846 - Potassium Phosphate, Monobasic Anhydrous Sterile Filtered
See Biochemicals Section for Complete Listing Miscible with Water
Components (mg/L)
P6780 - Potassium Sodium Tartrate
Sodium Chloride 8500
Rochelle Salt, Seignette Salt, L(+)-Tartaric acid Potassium
Sodium salt Tween 20 0.2 mL
A component of Fehlings solution, used to differentiate Available Package Sizes
between water-soluble carbohydrate and ketone functional
100mL 500mL 1L
groups, and as a test for monosaccharides. Fehlings solution can
be used to determine whether a carbonyl-containing compound S7400 - Safranin O
is an aldehyde or a ketone.
See Biochemicals Section for Complete Listing
Powder
CAS Number: 6381-59-5
Formula: C4H4KNaO6 4H2O
Molecular Weight: 282.22
Soluble in Water
Store at Room Temperature
Available Package Sizes
100g 500g 1kg

Page 170 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
Seed Testing Papers, Round T8092 - Toluidine Blue O
Whatman Seed Germination Papers. Tolonium Chloride, C.I. 52040
100 per pack. A biological stain.
Pore Size: 4-7 m Powder
Average Width: 180 m CAS Number: 92-31-9
Formula: C15H16ClN3S
Molecular Weight: 305.83
Soluble in Water
Store at Room Temperature
Available Package Sizes
1g 5g 25g 100g

T7968 - Trehalose Dihydrate


-D-Glucopyranosyl--D-glucopyranoside
Used as a carbon source in the PhytoSelect Basal Media (P6800)
to select for Xanthomonas hortorum.
Powder
CAS Number: 6138-23-4
Formula: C12H22O112H2O
Product Number Paper Size Available Package Sizes Molecular Weight: 378.33
P6635 70 mm 1 Each Soluble in Water
P6645 90 mm 1 Each Store at Room Temperature

Seed Testing
Available Package Sizes
S515 - Sodium Phosphate, Monobasic Monohydrate 5g 25g 100g
See Biochemicals Section for Complete Listing
T8164 - Triphenyl Tetrazolium Chloride (TTC)
S744 - d-Sorbitol 2,3,5-Triphenyl-tetrazolium chloride, Tetrazolium chloride
See Biochemicals Section for Complete Listing
Powder
S804 - Sulfuric Acid Solution 1.0 N CAS Number: 298-96-4
Formula: C19H15ClN4
See Biochemicals Section for Complete Listing Molecular Weight: 334.8
Soluble in Water
T8199 - Tetrazolium Blue
Store at 2 to 6 C
See Biochemicals Section for Complete Listing
Available Package Sizes
T7982 - Thiourea 5g 10g 25g 100g
Thiocarbamide, Sulfourea
Thiourea is commonly used to increase the stress tolerance T8110 - Tyrothricin
in plants, and also to increase germination rate or to break Also known as Tyrothicinum.
dormancy in seeds. It has been used as a substitute for An antibiotic used with PhytoSelect Basal Medium (P6800); a
stratification in a number of tree species. combination of about 20% Gramicidin and 80% Tyrocidine.
Powder Powder
CAS Number: 62-56-6 CAS Number: 1404-88-2
Formula: CH4N2S Formula: C65H85N11O13
Molecular Weight: 76.12 Molecular Weight: 1228.44
Soluble in Water Soluble in Ethanol
Store at Room Temperature Store at Room Temperature
Available Package Sizes Available Package Sizes
25g 100g 500g 1g 5g 25g

For More Information on Stains & Dyes


See "Figure 35. Stains & Dyes Usage Table" on page 264 in
the Technical Section.

Seed Testing A-Z Page 171


Product Catalogue & Laboratory Guide
T8020 - Tyrothricin Solution (10 mg/mL)
A solution of Tyrothricin (T8110) - also known as
Tyrothicinum - at 10 milligrams per milliliter of Ethanol. An
antibiotic used with PhytoSelect Basal Medium (P6800); a
combination of about 20% Gramicidin and 80% Tyrocidine.
Liquid
CAS Number: 1404-88-2
Formula: C65H85N11O13
Molecular Weight: 1228.44
Miscible with Water
Store at -20 to 0C
Available Package Sizes
10mL 25mL 100mL

X8454 - XTS Medium


A semi-selective medium for the isolation of Xanthomonas
translucens from barley and wheat seeds as described by Schaad
and Forster (1985).
Store at Room Temperature
Soluble in Water
Use at 28.0 grams per liter of medium
Components (mg/L)
Seed Testing

Meat Extract 3000


Peptone 5000
Agar 15,000
D-Glucose, Anhydrous 5000
Available Package Sizes
1L 500g

Find Plant Tissue Culture Media on Pages 57 to 130


MS Media
Crop Specific Media
Vitamin Solutions
Orchid Media
Stock Solutions

Page 172 2015 PhytoTechnology Laboratories


Product Catalogue & Laboratory Guide
Phycology B1411 - Blue-Green Medium (BG-11)
PhytoTechnology Laboratories has recently 50x Concentrate Solution
introduced a series of products for phycology research. A growth medium for freshwater cyanobacteria as described by
Stanier et al. (1971).
With media and reagents for freshwater and marine
This medium can be buffered with HEPES (H326), and is
algae and cyanobacteria, all manufactured to the same biologically tested with 20mM of HEPES per liter of medium.
levels of quality as our plant tissue culture media. Store at 2 to 6 C
Miscible with Water
Most components used in the formulation of Use at 20 milliliters per liter of medium
Phycology Media are available as individual Adjust to pH 8.0 0.1
Algal Culture Tested
components. Check the Biochemicals Section on
Page 15 for component product listings and more Components (mg/L)
information. Boric Acid 143
Calcium Chloride, Anhydrous 1359
Check www.phytotechlab.com frequently as we Citric Acid 300
continue to expand this product line. Cobalt Nitrate Hexahydrate 2.45
Cupric Sulfate Pentahydrate 3.95
A256 - Acetic Acid, Glacial Ferric Ammonium Citrate 300
See Biochemicals Section for Complete Listing Magnesium Sulfate, Anhydrous 3750
Manganese Chloride Tetrahydrate 90.5
B1511 - Blue-Green Medium (BG-11) Disodium Magnesium EDTA 50
Phycology

A growth medium for freshwater cyanobacteria as described by Potassium Phosphate, Dibasic 2000
Stanier et al. (1971).
Sodium Carbonate, Anhydrous 1000
This medium can be buffered with HEPES (H326), and is
biologically tested with 20mM of HEPES per liter of medium. Sodium Molybdate Dihydrate 19.5
Store at 2 to 6 C Sodium Nitrate 75000
Soluble in Water Zinc Sulfate Heptahydrate 11.1
Use at 1.68 grams per liter of medium Approximate pH at Room Temperature 4.2 0.5
Adjust to pH 8.0 0.1
Algal Culture Tested Available Package Sizes
Components (mg/L) 1L 2L
Boric Acid 2.86
Calcium Chloride, Anhydrous 27.18
Citric Acid 6.0
Cobalt Nitrate Hexahydrate 0.049
Cupric Sulfate Pentahydrate 0.079
Ferric Ammonium Citrate 6.0
Magnesium Sulfate, Anhydrous 75.0
Manganese Chloride Tetrahydrate 1.81
Disodium Magnesium EDTA 1.0
Potassium Phosphate, Dibasic 40.0
Sodium Carbonate, Anhydrous 20.0
Sodium Molybdate Dihydrate 0.39
Sodium Nitrate 1500.0
Zinc Sulfate Heptahydrate 0.222
Approximate pH at Room Temperature 8.0 1.0
Available Package Sizes
1L 10L 50L

Page 174 2015 PhytoTechnology Laboratories


www. p h y t o t e c h l a b .c o m
B1675 - Bolds Basal Medium (BBM) B1650 - Bolds Basal Medium (BBM)
A traditional freshwater algal medium for axenic maintenance 50x Concentrate Solution
as described by Nichols & Bold (1965). A freshwater algal standard medium as described by Nichols &
1.0 mL of 0.1% Sulfuric Acid Solution (S7664) to be added per Bold (1965).
liter of media at 1x concentration.
Store at 2 to 6 C
Store at 2 to 6 C Miscible with Water
Soluble in Water Use at 20.0 mL per liter of medium
Use at 0.705 grams per liter of medium Adjust to pH 6.6 0.1
Adjust to pH 6.6 0.1 Algal Culture Tested
Algal Culture Tested
Components (mg/L)
Components (mg/L)
Boric Acid 571
Boric Acid 11.42
Calcium Chloride, Anhydrous 944
Calcium Chloride, Anhydrous 18.87
Cobalt Nitrate Hexahydrate 24.5
Cobalt Nitrate Hexahydrate 0.49
Cupric Sulfate Pentahydrate 78.5
Cupric Sulfate Pentahydrate 1.57
EDTA, Disodium Salt 3181
EDTA, Disodium Salt 63.69
Ferrous Sulfate Heptahydrate 249
Ferrous Sulfate Heptahydrate 4.98
Magnesium Sulfate, Anhydrous 3750
Magnesium Sulfate, Anhydrous 36.63
Manganese Chloride Tetrahydrate 72
Manganese Chloride Tetrahydrate 1.44
Potassium Hydroxide 2984
Potassium Hydroxide 31.0
Potassium Phosphate, Dibasic 1550
Potassium Phosphate, Dibasic 75.0
Potassium Phosphate, Monobasic 3750

Phycology
Potassium Phosphate, Monobasic 175.0
Sodium Chloride 8750
Sodium Chloride 25.0
Sodium Molybdate 1250
Sodium Molybdate 1.19
Sodium Nitrate 12550
Sodium Nitrate 250.0
Zinc Sulfate Heptahydrate 441
Zinc Sulfate Heptahydrate 8.82
Approximate pH at Room Temperature 4.2 0.5
Approximate pH at Room Temperature 8.0 2.0
Available Package Sizes
Available Package Sizes 500mL 1L 2L
1L 10L 50L
C2124 - Cobalt Sulfate, Heptahydrate
Plant Tissue Culture Tested
Powder
CAS Number: 10026-24-1
Formula: CoSO4 7H2O
Molecular Weight: 281.103
Soluble in Water
Store at Room Temperature
Merck 13, 2473
Available Package Sizes
25g 100g

Phycology A-Z Page 175


Product Catalogue & Laboratory Guide
F3222 - f/2 Guillards Marine Enriched Seawater G3454 - f/2 Guillards Marine Enriched Seawater
A powder formulation of the vitamins, macro- and trace without Silicate
element solutions as described by Guillard and Ryther (1962). A powder formulation of the vitamins, macro- and trace
To be dissolved into Seawater . Contains silicate for diatom element solutions as described by Guillard and Ryther (1962).
growth. This product does not contain silicate, and can therefore be
Store at 2 to 6 C used for the axenic maintenance of non-diatom marine algae.
Partially Soluble in Seawater To be dissolved into Seawater .
Use at 0.1 grams per liter of medium Store at 2 to 6 C
Adjust to pH 7.5 0.5 Partially Soluble in Seawater
Algal Culture Tested Use at 0.09 grams per liter of medium
Components (mg/L) Adjust to pH 7.5 0.5
Sodium Nitrate 75.0 Algal Culture Tested
Cobalt Chloride Hexahydrate 0.01 Components (mg/L)
Cupric Sulfate Pentahydrate 0.01 Sodium Nitrate 75.0
EDTA, Disodium Salt 4.36 Cobalt Chloride Hexahydrate 0.01
Ferric Chloride, Anhydrous 1.9 Cupric Sulfate Pentahydrate 0.01
Manganese Chloride Tetrahydrate 0.18 EDTA, Disodium Salt 4.36
Molybdic Acid, Disodium Salt 0.006 Ferric Chloride, Anhydrous 1.9
Sodium Phosphate, Monobasic 5.0 Manganese Chloride Tetrahydrate 0.18
Sodium Metasilicate, Anhydrous 12.94 Molybdic Acid, Disodium Salt 0.006
Zinc Sulfate, Heptahydrate 0.022 Sodium Phosphate, Monobasic 5.0
Phycology

Biotin 0.0005 Zinc Sulfate, Heptahydrate 0.022


Cyanocobalamin, Vitamin B12 0.0005 Biotin 0.0005
Thiamine Hydrochloride 0.1 Cyanocobalamin, Vitamin B12 0.0005
Approximate pH at Room Temperature 6.0 2.0 Thiamine Hydrochloride 0.1
Approximate pH at Room Temperature 6.0 2.0
Available Package Sizes
1L 10L 50L Available Package Sizes
10L 50L

F3138 - Ferric Ammonium Sulfate


Plant Tissue Culture Tested
Powder
Custom Media & Services CAS Number: 7783-83-7
PhytoTechnology Laboratories Formula: NH4Fe(SO4)2 12H2O
offers custom manufacturing Molecular Weight: 482.25
and testing services. For more Soluble in Water
information please see page Store at Room Temperature
12.