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V max [S] V 0 V max V 0 K m 1 2 V max
V max [S] V 0 V max V 0 K m 1 2 V max
V max [S] V 0 V max V 0 K m 1 2 V max
V max [S]
V 0 V max
V 0
K m
1
2 V max
K m
[S] (mM)
V 0 ( M/min)
(a) Competitive inhibition E S ES E P S S I K I EI I
(a)
Competitive inhibition
E
S
ES
E P
S
S
I
K
I
EI
I
I
(b)
Uncompetitive inhibition
E
S
ES
E P
I
S
K
S
I
ESI
I
I
S
(c)
Mixed inhibition
E
S
ES
E P
I
I
S
K
K
S
I
I
EI
S
ESI
I
I
S
S
I
I
V max 1 2 V max
V max
1
2 V max

K 0.5

[S] (mM)

V max 1 V max 2 K 0.5 K 0.5 K 0.5
V max
1
V max
2
K 0.5
K 0.5
K 0.5

[S] (mM)

V max V max V max 1 2 V max
V max
V max
V max
1
2 V max
No inhibitor Acetazolamide
No inhibitor
Acetazolamide
Reaction 2: ATP → ADP P i Reaction 3: Glucose ATP → glucose 6-phosphate ADP
Reaction 2:
ATP → ADP P i
Reaction 3:
Glucose ATP →
glucose 6-phosphate ADP
Reaction 1:
Glucose P i →
glucose 6-phosphate
∆G 2
∆G 3
∆G 1
∆G 3 = ∆G 1 ∆G 2
[I] 2 3 [I] 1.5 2 1 1 No inhibitor 1 1 K m Slope
[I]
2
3
[I]
1.5
2
1
1
No inhibitor
1
1
K m
Slope
K m
V max
V max
No inhibitor
1 0 M /min( 1
)
V
1
)
V 1 0
M/min(
1 0 M /min( 1
)
V
Inhibitor type Apparent V max Apparent K m None V max Competitive V max Uncompetitive
Inhibitor type
Apparent V max
Apparent K m
None
V max
Competitive
V max
Uncompetitive
Mixed
V max /
V max /
K m
K m
K m /
K m /
Activation barrier
(transition state, ‡)
G
uncat
Reactants (A)
G
cat
G
Products (B)
Stored Other nutrients cellular work Ingested Complex foods biomolecules Solar Mechanical photons work Osmotic
Stored
Other
nutrients
cellular work
Ingested
Complex
foods
biomolecules
Solar
Mechanical
photons
work
Osmotic
work
ADP
2
Catabolic
HPO 4
Anabolic
reaction
reaction
pathways
pathways
exergonic)
(endergonic)
ATP