Академический Документы
Профессиональный Документы
Культура Документы
1. Principle of MALDI-TOF
Mass spectrometry is a technique used to identify a molecule and determine its chemical structure
by analyzing the mass and the charge of its ions. One of the uses of MALDI-TOF* mass spectrometry
is to determine the elemental composition of a sample.
MALDI stands for "Matrix Assisted Laser Desorption/Ionization". The sample for MALDI
is uniformly mixed in a large quantity of matrix. The matrix absorbs the ultraviole t light
(nitrogen laser light, wavelength 337 nm) and converts it to heat energy. A small part of
the matrix (down to 100 nm from the top outer surface of the Analyte in the diagram)
heats rapidly (in several nano seconds) and is vaporized, together with t he sample.
Fast and reliable identification of microbes which pose threats as agents of bioterrorism is required,
not only to combat biological-warfare attacks, but also to prevent natural outbreaks caused by these
organisms. Conventionally, organisms which pose severe threats as agents of bioterrorism have
been identified by phenotypic, genotypic, and immunological identification systems which are slow,
cumbersome and pose significant risk to the laboratory personnel. Recently various researchers
reported MALDI-TOF MS as a simple, rapid and reliable approach to identify highly pathogenic
organisms like Brucella spp., Coxiella burnetti, Bacillus anthracis, Francisella tularensis, and Y.
pestis (Shaw et al., 2004; Pierce et al., 2007; Lasch et al., 2009; Ayyadurai et al., 2010; Seibold et
al., 2010; Lista et al., 2011; Vranakis et al., 2013).
References:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4525378/
https://www.ncbi.nlm.nih.gov/pubmed/21133692
http://www.biomerieux.com/en/maldi-tof-mass-spectrometry
http://www.shimadzu.com/an/lifescience/maldi/princpl1.html