in China people people

Altogether with country

country Family Mark
quasi-
GB 47892-2016

National Food Safety Standard

Microbiological examination of
food Determination of
the total number of colonies

2016-12-23re lease 2017-06-

23Imp l eme nt
 National Health and Family Planning Commission
People's Republic of China release
country Family food Article medicine Article
Prison Supervise tube Reasontotal Office

beforeSpeech

Instead of the standard National Food Safety Standard Microbiological examination of

food Determination of the total number of colonies
 National Food Safety Standard
Microbiological examination of food Determination of the
total number of colonies

1 range

This standard specifi es the total number of bacteria

in food The
standard method of measuring the total number of
colonies measured for food in

2 Terms and Defi nitions

T he t ot a l nu m be r o f c o lon ies aerobicplatecount

The resulting sample after processed food sample under conditions such as the
temperature of the culture medium and culture time per culture The
total number of colonies of microorganisms formed in the subject sample

3 Equipment and materials

In addition to the microbiological laboratory culture apparatus and other

conventional sterilization equipment and materials are described below
31 Incubator
32 refrigerator
33 Constant temperature water bath
34 A sense of balance amount
35 Homogenizer
36 Oscillator
37 Sterile pipette Equipment Graduation Equipment Scale or pipette tip
and
38 Sterile conical fl ask capacity
39 Diameter sterile petri dish
310 Meter or Colorimetric tubes or precision Dipstick
311 And a magnifying glass or a colony counter

4 Media and Reagents

41 See Plate Count agar

42 Phosphate buff er, see
43 See sterile saline

5 Inspection procedures

The total number of colonies in Figure inspection procedures

 Map 1 The total number of colonies inspection procedures

6 Steps

61 Sample dilution

611 Solid and semi-solid samples weighing Home fi lled with sample
Phosph ate bu ff ered sali ne solu tion or ster ile in ner
cup homogen eous homogeneous Or placed fi lled with
Sterile diluent bag with homogeneous slap formula
Homogenizer beat production The sample was homogenized
612 Liquid sample to draw sterile pipette Home fi lled with sample
P hosp hat e bu ff e red salin e so lu tion o r an
a pp rop riate nu mber of p re -sterile E rlen meyer fl ask a nd mix well s ter ile
g lass beads ma de The sample was homogenized
613 use Sterile pipette or micropipette draw Samples were homogenized
Note slowly along the wall to be fi lled with Sterile tube dilution with
attention pipette tip or tips do not touch the liquid surface was diluted Tubes
were shaken or switch Su pport steri le pip ett e rep eat ed
pip ett ing it
Formed uniformly mixed The sample was homogenized
614 press Preparation of operation Fold serial diluted sample was
homogenizedEvery incremental change with diluted Secondary Sterile
pipette tips, or
615 According to estimates of the pollution of the sample selection More A
liquid sample was homogenized sample dilutions may comprise a suitable liquid
Making Fold dilution increments draw
Homogenized sample was done in two plates per dilution sterile petri
dish Simultaneously draw
Blank dilution to a sterile petri dish in two as control
616 Promptly Cool to The plate count agar medium can be placed in
Constant temperature water bath and incubated pour
plate rotatable plate for homogenization

62 to cultivate

621 Will be solidifi ed agar plates fl ipped to cultivate Aquatic products

to cultivate
622 It may cover a thin layer of agar agar surface after solidifi cation if the
sample might contain colony growth medium top agar was fi lled about Flip
press plate after solidifi cation Conditions were cultured

63 Colony counts

631 Available recording dilution and corresponding number of colonies or colony

counter with a magnifying glass when necessary, visually Colony
counts in colony forming units Show
632 Select the number of colonies Total plate
count of colonies grown colonies no spread between Below the plate is greater
than the number of recording specifi c colonies It may be recorded as
uncountable The number of colonies for each dilution should be
the mean of the two plates
633 One of the plates has grown larger sheet-like colonies and should not be
used in a non-fl at sheet-like colonies should grow as the number of colonies
dilution if less than half the sheet-like plate while the remaining half of the
colonies were very uniform distribution of colonies i.e. after half of the tablet
may be calculated by multiplying
On behalf of a fl at
colonies
634 When no distinct boundary between the colonies that appeared on the plate
chain will grow each single strand as a colony count

7 Results and Reports

71 The method of calculating the total number of colonies

711 If only a dil uti on of the number of colonies on the plate of the two plates
calcul ate the average number of colonies counte d i n the appropriate range
and the n multipl ied by the correspondi ng diluti on factor as the average of
each of The total number of colonies in the sample
results
712 If the number of two successive plates of colonies upon dilution in a suitable
range according to the count of formula Compute
 C
N =
n +.nd
Where
N Samples colonies
C The number of plates containing the appropriate range of plate culture, and
the number of colonies
n The fi rst dilution low
dilution multiple of the number
plate n The second high dilution
dilution plate number d The
fi rst dilution dilution factor
Examples

Dilution The fi rst dilution The second

dilution
Colonies
 C + + +
N = = = =
n +.nd . .
+ -
 Said data by After rounding numbers expressed as or
713 If all dilutions on a plate are greater than the number of colonies The
hig hes t d ilu tion pla tes were count ed in the other pl ate may be reco rded as a
res u lt uncou ntab le number of colon ies av erag e ca lcu lat ed by th e maximum
dil utio n f actor
714 When the number of plates of colonies were smaller than all dilutions The
lowest average number of colonies should dilutions calculated by multiplying the dilution factor
715 If the liquid sample comprises all dilutions was no colony growth plate stock
places less than Multiplied by the
minimum dilution factor is calculated
716 When the number of plate cultu re not in all dilutions
Wherein a portion of less than between
Greater than or places closest or
The average colony number calculated by
multiplying the dilution factor
72 Total number of colonies

721 Less than the number of colonies When you press the rounded-off numbers
rounded to integer report
722 Is greater than or equal to the number of colonies The first
Using digits before rounding takes the rounded-off Later digit
bits can also be used in place of The exponent is represented by rounded-off
after rounding the use of two signifi cant fi gures
723 If the tablet is spread all colonies can not count the colonies spread report
724 If there is a blank colony growth is the result of the detection is invalid
725 Weighing sampled Reported in units of volume
sampled Units report

Attached record A
Media and Reagents

A1 Plat e Co unt Ag ar platecountagarPCA Medium

A11 ingredient

Trypton
e
glucose
yeast
extract
Agar
Distilled
water

A12 Method
The above components were dissolved by boiling in distilled water and added to
adjust to Dispensing tube or
E r l e n m e y e r fl a s k Autoclave

A2 Phosphate buff er
A21 ingredient

Distilled
water,
potassiu
m
dihydroge
n
phosphat
e

A22 Method

Stock Of potassium Distilled With of Sodium

solution
- hydraulic dihydrogen
Diluted water
with distilled After stored about hydroxide
control
Stock to water
solution to take
dilution in a Diluted with distilled water to
Dispensed into a suitable container Autoclave

A3 Sterile saline
A31
ingredient

Sodiu
m chloride
in distilled
water
A32
Method
Weigh Sodium chloride was dissolved Distilled water Autoclave