Stage Image Description % in BM

Myeloblast Size: 15- 20 um 02%

Nucleus: Round to oval
Nucleoli: 2-5
Chromatin: Fine
Cytoplasm: Moderate basophilia
Granules: Absent or up to 20
N/C ratio: 4:1

Promyelocyte Size: 14-24 um 25 %

Nucleus: Round to oval
Nucleoli: 1-3 or more
Chromatin: Fine, but slightly coarser than myeloblast
Cytoplasm: Basophilic
Granules:
Primary: >20 to many; colored red to purple or burgundy
Secondary: None
N/C ratio: 3:1
Neutrophilic myelocyte Size: 12 18 um 5 19 %
Nucleus: Round to oval, slightly eccentric
Nucleoli: Usually not visible
Chromatin: Coarse and more condensed than promyelocyte
Cytoplasm: Slightly basophilic to cream-colored
Granules:
Primary: Few to moderate
Secondary: Variable number; become predominant as cell
matures
N/C ratio: 2:1
Neutrophilic Size: 10 15 um 13 22 %
metamyelocyte Nucleus: Indented; kidney bean shape
Nucleoli: Not visible
Chromatin: Moderately clumped
Cytoplasm: Pale pink to cream colored to colorless
Granules:
Primary: Few
Secondary: May
N/C ratio : 1.5:1
Neutrophilic band Size: 10-15 um 17 33 %
Nucleus: Constricted but no threadlike filament
Nucleoli: Not visible
Chromatin: Coarse, clumped
Cytoplasm: Pale blue to pink
Granules:
Primary: Few
Secondary: Abundant
N/C ratio : Cytoplasm predominates
Segmented neutrophil Size: 10 15 um 3 11 %
Nucleus: 2-5 lobes connected by thin filaments without visible
chromatin
Nucleoli: Not visible
Chromatin: Coarse, clumped
Cytoplasm: Pale pink, cream-colored or colorless
Granules:
Primary: Rare
Secondary: Abundant
N/C ratio : Cytoplasm predominates
Stage Image Description % in BM
Myeloblast Size: 15- 20 um 02%
Nucleus: Round to oval
Nucleoli: 2-5
Chromatin: fine
Cytoplasm: moderate basophilia
Granules: absent or up to 20
N:C ratio : 4:1
Promyelocyte Size: 14-24 um 25 %
Nucleus: Round to oval
Nucleoli: 1-3 or more
Chromatin: Fine, but slightly coarser than myeloblast
Cytoplasm: basophilic
Granules:
Primary: >20 to many; colored red to purple or burgundy
None
Secondary: 3:1
N:C ratio :

Basophilic Rarely seen 5 19 %

myelocyte

Basophilic Rarely seen 13 22 %

metamyelocyte

Basophilic Rarely seen 17 33 %

band

Basophil Size: 10-14 um <1%

Nucleus: Usually 2 lobes connected by thin filaments without
visible chromatin
Nucleoli: Not visible
Chromatin: Coarse, clumped
Cytoplasm: Lavender to colorless
Granules:
Primary: Rare
Secondary: Variable in number with uneven distribution, may obscure
nucleus; deep purple to black; irregularly shaped
N:C ratio : Cytoplasm predominates
Stage Image Description % in BM
Monoblast Size: 12-18 um Not defined
Nucleus: Round to oval; may be irregular shaped
Nucleoli: 1-2; may not be visible
Chromatin: Fine
Cytoplasm: Light blue to gray
Granules: None
N:C ratio : 4:1
Promonocyte Size: 12-20 um <1%
Nucleus: Irregularly shaped; folded
Nucleoli: May or may not be visible
Chromatin: Fine to lacy
Cytoplasm: Light blue to gray
Granules: Fine azurophilic (burgundy colored)
N:C ratio : 2-3:1

Monocyte Size: 12-20 um 2%

Nucleus: Variable; may be round, horseshoe shaped or kidney shaped
Nucleoli: Not visible
Chromatin: Lacy
Cytoplasm: Blue-gray, may have pseudopods
Granules: Many fine granules ; ground glass appearance
Vacuoles: Absent to numerous
N:C ratio : Variable

Macrophage Size: 15-80 um Macrophages

Nucleus: Eccentric, kidney or egg-shaped, indented or elongated reside in
Nucleoli: 1-2 tissues such
as bone
Chromatin: Fine, dispersed
marrow
Cytoplasm: Abundant with irregular borders; may contain ingested material
Granules: Many coarse azurophilic
Vacuoles: May be present
Stage Image Description % in BM
Megakaryoblast Size: 10-24 um 20% of
Nucleus: Round megakaryocyte
Nucleoli: 2-6 precursors in
bone marrow
Chromatin: Homogenous, loosely organized
Cytoplasm: Basophilic
Granules: Absent by wright stain
N:C ratio : 3:1
Promegakaryocyte Size: 15-40 um 25% of
Nucleus: Indented megakaryocyte
Nucleoli: Variable precursors in
bone marrow
Chromatin: Condensed
Cytoplasm: Basophilic
Granules: Present
N:C ratio: 1:2
Megakaryocyte Size: 20-90 um 5-10 per 10x
Nucleus: 2-32 lobes (8 lobes: most coomon) objective (x100
The size of the cell varies according to number of lobes magnification) 1-
2 per 50x
present
objective (x500
Cytoplasm: Blue to pink; abundant magnification)
Granules: Reddish to blue; few to abundant
N:C ratio: Variable

Platelets Size: 2-4 um NA

Nucleus: NA
Cytoplasm: Light blue to colorless
Granules: Red to violet, abundant
N/C ratio: NA
Stage Image Description % in BM
Lymphoblast Size: 10-20 um Not
Nucleus: Round to oval defined
Nucleoli: 1
Chromatin: Fine, evenly stained
Cytoplasm: Scant, slightly to moderately basophilic
Granules: None
N:C ratio : 7:1 to 4:1
Prolymphocyte Size: 9-18 um Not
Nucleus: Round or indented defined
Nucleoli: 0-1; usually single, prominent, large nucleolus
Chromatin: Slightly clumped, intermediate between lymphoblast and mature
lymphocyte
Cytoplasm: Light blue
Granules: None
N:C ratio : 3-4:1
Lymphocyte Size: 7-18 um 5 to 15
Nucleus: Round to oval; may be slightly indented %
Nucleoli: Occasional
Chromatin: Condensed; clumped; blocky, smudged
Cytoplasm: Scant to moderate; sky blue; vacuoles may be present
Granules: None in small lymphocyte; may be a few azurophilic in larger
lymphocytes; if granules are prominent, the cell is called a large
granular lymphocyte
N:C ratio : 5:1 to 2:1
Plasma cell Size: 8-20 um 0 to 1 %
Nucleus: Round or oval, eccentric
Nucleoli: None
Chromatin: Coarse
Cytoplasm: Deeply basophilic, often with perinuclear clear zone (hof)
Granules: None
Vacuoles: None to several
N/C ratio: 2:1 to 1:1