School logo Name of the University

Address
College of Medical Technology
Learning Plan/Instructors Guide in Clinical Chemistry1
VISION Vision of the College of Medical Technology

Mission of the College of Medical Technology

MISSION

INSTITUTIONAL CORE VALUES Institutional Commitment:

 Institutional Intended Learning Outcomes (ILOs) Student Outcomes (SO) or SLO
SO # 1.
ILO 1: demonstrate technical competence in the performance of clinical laboratory tests in aid
Description: of diagnosis, treatment and management of diseases vis--vis biosafety and waste
management;
ILO 2 :

Description: Performance Indicators (PI)

ILO 3: The graduates shall be able to:

Description:

ILO 4:
skills and technology;
Description:
sting procedures, equipment and professional/ technical competency
Program Educational Objectives (PEO) using quality assurance methodologies;
At the end of the program, the graduates of Bachelor of
ILO1 ILO2 ILO3 ILO 4
Science in Medical Laboratory Science shall have: maintenance;
1. developed the knowledge, skills, and professional regulations;
attitude in the performance of clinical laboratory procedures
needed to help the physician in the proper diagnosis,
treatment, prognosis and prevention of diseases;
instruments; and
rinciples of educational methodology and laboratory management.
2. developed critical thinking skills in Medical
Technology/Medical Laboratory Science. .

3. appreciated the need for research and community-

related activities; SO# 4
4. participated in activities related to promoting the
profession and actively engage in lifelong learning Demonstrate entrepreneurship skills in areas related to Medical Technology practice
activities; and
 5. developed collaborative and leadership qualities Performance Indicators (PI)

The graduates shall be able to:

SO # 2. management;
Demonstrate critical thinking skills in the workplace
Performance Indicators (PI)

SO # 5..
The graduates shall be able to: Demonstrate inter-personal skills, leadership qualities and ethical
lity assurance and continuous quality improvement; practice of the profession

reporting; Performance Indicators (PI)

The graduates shall be able to:
action; and
-disciplinary and multi-cultural team;
apply the principles of educational methodology and resource management.
-personal skills with patients;

SO # 3
Contribute in the collection, analysis and projection of health information for he principles of data security and patients confidentiality; and
improving the health care management system

Performance Indicators (PI)

The graduates shall be able to:

and issues.
 SO # 6 Demonstrate research skills in relevant areas of Med Tech practice SO# 7 Engage in community-oriented activities

Performance Indicators (PI) Performance Indicators (PI)

The graduates shall be able to:
The graduates shall be able to:
-oriented activities;
arch topic relevant to medical technology; -related activities in the community; and
ent, monitor and evaluate activities in the community.

Logo of the
SO # 8. Engage in lifelong learning activities SO # 9. Demonstrate effective communication skills

Performance Indicators (PI) Performance Indicators (PI)

The graduates shall be able to:
The graduates shall be able to:
patients.

ional organization; and

.
SchoolLogo Name of the School
Address

College of Medical Technology/Medical Laboratory Science

Learning Plan/ Instructors Guide in Clinical Chemistry 1

Course Title: Clinical Chemistry 1

Course Description:

The course deals with the quantitative measurement of biochemical substances found in body fluids essentially blood. This involves the knowledge and understanding of the basic concepts and principles of their metabolism,
laboratory analyses, and pathophysiology. Quality assurance and safety are given due emphasis.

Course Credit: 4 units (3 units lecture, 1 unit laboratory)

Contact Hours: 3 hours lecture, 3 hours laboratory per week (54 hours lecture ad 54 hours laboratory per semester)

Prerequisites: Biochemistry, Human Anatomy and Physiology

Placement: Third Year, 1st Semester

Course Learning Outcomes :

At the end of this course, the student is able to:
1. Explain the different biochemical substances, their metabolism, actions, reference ranges and variables that may affect their analyses
2. Correlate test results with pathologic conditions
3. Apply concepts and principles of instrumentation in the laboratory
4. Perform basic laboratory calculations
5. Practice quality assurance and laboratory safety
6. Perform correctly laboratory essays used to measure concentration of specific analyses
7. Solve common problems encountered in the clinical laboratory
8. Manifest professionalism
References: Latest Edition

1. Anderson, Shauna and Susan Cockyane. Clinical Chemistry: Concepts and Applications. USA: Waveland Press Inc., 2007.
2. Arneson, W. and J. Brickell. Clinical Chemistry: A Laboratory Perspective. USA: F.A. Davis Co., 2007.
3. Ashwood E., D. Bruns and C. Burtis. Tietzs Fundamentals of Clinical Chemistry 6th ed. PhiladelphiaL W.B. Saunders Co., 2007.
4. Ashwood E., D. Bruns and C. Burtis. Tietzs Textbook of Clinical Chemistry and Molecular Diagnostics 4th ed. Philadelphia: W.B. Saunders Co., 2007.
5. Bishop, Michael L. et.al. Clinical Chemistry: Principles, Procedures, Correlations, 5th ed. Philadelphia: Lippincott Williams, Philadelphia, 2005.
6. Crook, Martin. Clinical Chemistry and Metabolic Medicine 7th ed. USA: Hodder Arnold Publication, 2006.
7. Furr, Keith. CRC Handbook of Laboratory Safety 5th ed. USA: CRC, 2000.
8. Garza, Diana and Kathleen Becan-McBride. Phlebotomy Handbook: Blood Collection Essentials 7th ed. USA: Prentice Hall, 2004.
9. Helms, Joel R. Mathematics for Medical and Clinical Laboratory Professionals. USA: Delmar Learning, 2008.
10. Hibbert, Brynn. Quality Assurance in the Analytical Chemistry Laboratory. USA: Oxford University Press, 2007.
11. Hoeltke, Lynn. Phlebotomy: Principles and Procedures. USA: Delmar Learning, 2006.
12. Kaplan, A., A. Pesce and S. Kazmierczak. Clinical Chemistry: Theory, Analysis, Correlation 4th ed. Edinburgh: Mosby, 2002.
13. Lewandrowsky, Kent. Clinical Chemistry: Laboratory Management & Clinical Correlations. USA: Lippincott Williams and Wilins 2002.
14. Marshall, William and Stephen Bangert. Clinical Chemistry 5th ed. Edinburgh: Mosby, 2004.
15. McClatchey, Kenneth. Clinical Laboratory Medicine 2nd ed. USA: Lippincott Williams and Wilkins, 2002.
16. Mcpherson, Richard A. and Matthew R. Pincus. Henrys Clinical Diagnosis and Management by Laboratory Methods 21st ed. Philadelphia: Elsevier Inc., 2007.
17. Prichard, Elizabeth and Victoria Barwick. Quality Assurance in Analytical Chemistry. USA: Wiley-Interscience, 2007.
18. Sacher, Ronald and Richard McPherson. Widmanns Clinical Interpretation of Laboratory Tests 11th ed. Thailand: F.A. Davis, 2000.
19. Scott M., A. Gronowski and C. Eby. Tietzs Applied Laboratory Medicine 2nd ed. USA: Wiley-Liss, 2007.
20. Wu, Allan. Tietzs Clinical Guide to Laboratory Tests 4th ed. Philadelphia: W.B. Saunders, 2006.

Electronic References:
1. http://webpages.chhs.niu.edu/williams/AHP318/ClinicalChem.htm
2. http://www.dgrhoads.com/links.shtml
3. http://www.kmcsystems.com/invitro_clinical.asp#1
4. http://www.ualberta.ca~intd410/departments/spoc.html
5. http://www2.apsu.edu/~thompsonj/clin-chem-page1.htm
 Unit Learning Outcomes Teaching Learning Resources
Values Assessment Tasks
( ULOs) Content T.A. Activities
Deployed
Lecture Laboratory Assessment Tool
At the end of this unit, the student is I. Introduction Didactic Accuracy
able to: Lecture: Hrs Method Demo and Honesty MCQ Textbooks
1. Define common terms used 1. Scope of Clinical Chemistry Lecture Return Demo Responsibility Answer Key
2. Identify the duties of a Med Tech a. Overview Accountability E-books
in Clinical Chemistry section b. Importance Interactive Demonstration
3. Describe the functions of the c. Role of Medical Technologist Group of pipetting PPT
measuring devices in terms of d. Definition of terms Discussion technique and Identification
when and how to use them (Cooperativ volume Laboratory Manual
4. Cite the differences between the Laboratory 4 hrs e) measurement
different measuring devices 1. Clinical Laboratory Apparatus and
used in Clinical Chemistry Supplies Discussion of
5. Correctly used pipettes (for 2. Volume measurement - laboratory Answer Key
measurement and delivery of wares
liquid) & weighing balance - water &
chemicals
- supplies

At the end of this unit, the student is II. Laboratory Mathematics Interactive Demonstration Accuracy Problem Solving Rubrics Textbooks
able to: Lecture: 8 hrs Group Honesty
1. Compute for Normality, Molarity, 1. Laboratory Mathematics discussion Preparation of Responsibility Board Work E-books
% solutions a. Unit conversions solutions Accountability Or
2. Carry out unit conversions b. Percent solutions PPT
3. Prepare various dilutions of c. Normally Preparation of Seatwork
samples and chemical d. Molarity dilutions Laboratory Manual
e. Dilutions
f. pH and pOH

Laboratory: 4 hrs
1. preparation of solutions and dilutions
At the end of this unit, the student III. Laboratory Safety Interactive Demonstration Accuracy Board work Rubrics Textbooks
able to: Lecture: 3 hrs Group Honesty
1. Apply the universal precautions 1. Universal Precautions discussion Preparation of Responsibility Assignments E-books
2. Discuss the different laboratory 2. Laboratory Hazards solutions Accountability
hazards a. Biological Seatwork PPT
3. Demonstrate laboratory safety b. Chemical Preparation of
practices and proper waste c. Electrical dilutions Laboratory Manual
disposal d. Fire
4. Cite the significance of e. Radiation
laboratory waste management f. Others
3. Safety equipment
4. Laboratory waste management
a. Segregation
b. Storage
c. Treatment
d. Disposal

Laboratory: 3 hrs
1. Hand washing
2. Cleaning of glassware
3. Disinfection of working areas
4. Laboratory Waste management
5. Laboratory Hazards
At the end of this unit, the student is IV. Specimen Collection and Processing Interactive Demonstration Patience MCQ Answer Keys Textbooks
able to: Lecture: 6 hrs Group - skin pick Compassion TFQ
1. Establish guidelines on 1. Specimen Discussion - venipuncture Accountability MTQ E-books
acceptability of blood samples a. Types - arterial Responsibility Rubrics
submitted to clinical laboratory - Blood Discovery puncture Beneficence Case PPT
2. Cite the duties of a Medical - CSF Teaching Confidentiality presentati
technologists with regards to - Urine (Unknown Centrifugation Adherence to on Laboratory Manual
proper specimen collection, - Others to known) of blood standards of
processing and handling b. Collection & Labeling practice
3. Given a request slip, rationalize c. Handling, Transport Processing, Preparation of
the steps to be undertaken in Storage & Preservation Serum,
patient preparation and 2. Specimen Variables Plasma and
specimen collection, processing a. Pre-analytical Whole Blood
and handling - Patient identification and
4. Correctly perform venipuncture preparation
and finger-prick methods of - Anticoagulants and
blood collection preservatives
5. Enumerate the precautions to be b. Analytical
consider in proper specimen c. Post-analytical
collection
6. List the pre0analytical variables Laboratory: 7 hrs
that may affect laboratory 1. Blood Collection
analyses and effects of test 2. Skin Puncture
results 3. Venipuncture
7. Prepare serum/plasma 4. Syringe
5. Evacuated System
6. Arterial Puncture
7. Preparation of Serum, Plasma and
Whole Blood
8. Laboratory Safety
9. Laboratory Waste Management
At the end of this unit, the student is V. Quality Management Interactive Preparation of Patience Computati Rubrics on Case Study Textbooks
able to: Lecture: 5 hrs Group QC chart Compassion on with
1. Discuss the principles and 1. Introduction discussion Accountability given set Rubrics on QC Chart E-books
concepts and advantages of a. Importance Case study Responsibility of
automation 2. Definition of Terms Beneficence problems PPT
2. Classify the different types of 3. Statistics Confidentiality
automation used in Clinical a. Descriptive Adherence to Laboratory Manual
Chemistry - Mean standards of
3. Compute & establish the values - Median practice
of central tendencies, - Mode
dispersions (x, SD, CV, etc) - SD
4. Prepare guidelines on how to - CV
prevent the interference of pre- - Variance
analytic variables on test results - SEM
5. Explain the concepts of internal b. Inferential
and external quality control - T tests
programs - F tests
4. Quality Control Charts
a. Levey-Jennings
b. Westgard Rules
c. Six-sigma
d. Others
5. Intra-laboratory QC Monitoring
6. Proficiency Testing
7. Method Selection
8. Issues and Concerns

Laboratory: 4 hrs
1. Preparation and interpretation of QC
chart
2. Application of Westgard Rules
At the end of this unit, the student is VI. Instrumentation Didactic Engaged Patience MCQ Answer Keys Textbooks
able to: Lecture: 8 hrs Method Learning Accountability TFQ
1. Discuss the concepts and 1. Methods Lecture Responsibility MTQ E-books
principles of instrumentation a. Photometry Lab. Adherence to
2. Enumerate the components of b. Spectrophotometry Interactive Performance standards of PPT
each instrument and their uses - AAS Group operation Problem
3. Differentiate instruments - FES discussion Spectrophoto Solving Laboratory Manual
according to components, - Reflectance Spectrophotometry meter using
analyses measured & uses; c. Nephelometry Micro Reading Readings
operations d. Turbidimetry Teaching
4. Explain correct proper specimen e. Fluorometry on Use of
collection, handling & transport f. Electrophoresis Assignment available
according to tests requested g. Chromatography automated
5. Use spectrophotometer, UV-Vis h. Ultracentrifugation machines
& IR spectrophotometer i. Chemiluminiscence
6. Correctly label blood, j. Electrochemistry Calibration of
serum/plasma samples k. Immunochemistry instruments
l. Dry Chemistry
2. Automation Preventive
3. Point of care testing maintenance
 4. Current trends of laboratory
instruments
Laboratory:
1. Operation and maintenance of 4 hrs
Spectrophotometer and other
laboratory instruments
2. Preventive maintenance of laboratory
instruments

At the end of this unit, the student is VII. Carbohydrates MCQ Answer Keys Textbooks
able to: Lecture: 6 hrs Engaged
1. Define common terms 1. Biochemistry Discussion Method of Technical TFQ E-books
associated with carbohydrates a. Classification Learning or Accuracy Rubrics on Case Analysis
2. Describe carbohydrates b. Metabolism Inductive Collaborative Honesty MTQ PPT
according to composition, 2. Specimen Considerations Method on Confidentiality Rubrics on Lab. Performance
classification, chemical 3. Glucose Measurement Case Actual Lab. Responsibility Laboratory Manual
properties a. Methods Analysis performance Reliability
3. Discuss the metabolism of CHO b. Reference Range of glucose
in the body 4. Clinical Significance essays and
4. Explain the mode actions of a. Diabetes mellitus others
different hormones in the b. Hyperglycemia
maintenance of blood glucose c. Hypoglycemia Case analysis
levels d. Other related diseases
5. Discuss the different processes 5. Tests for Diabetes Mellitus
involve in the maintenance of
normal blood glucose level Laboratory: 7 hrs
6. Rationalize the consideration 1. Enzymatic methods for glucose
patient preparation, specimen 2. HbA1c
collection, transport and 3. Tolerance Tests
processing & handling 4. Laboratory Safety
7. Describe the specimen of 5. Laboratory Waste Management
choice, principle involved,
advantages of the different
laboratory methods of glucose
determination

At the end of this unit, the student is VIII. Non-Protein Nitrogen Compounds Interactive Actual Compassion MCQ Answer Keys Textbooks
able to: Lecture: 5 hrs Discussion performance Accuracy
1. Define common terms 1. Biochemistry of glucose Honesty TFQ E-books
associated with renal function a. Types Inductive assays and Confidentiality Rubrics on Case Analysis
tests - Urea Method on others Responsibility MTQ PPT
2. Enumerate the different non- - Creatinine Case Reliability Rubrics on Lab. Performance
protein nitrogen (NPN) - Uric acid Analysis Case analysis Laboratory Manual
 substances present in the blood - Ammonia
3. Discuss the sources, - Amino acid
metabolism, formation and - Others
excretion of uric acid, urea, b. Metabolism
creatinine and ammonia 2. Specimen Considerations
4. Discuss the different factors that
may affect the level of uric, urea, 3. NPN Measurement
creatinine and ammonia in the a. Methods
blood b. Reference Range
5. Rationalize the requirements of 4. Clinical Significance
specimen collection, transport, a. Uremia
processing and handling b. Azotemia
6. Describe the principle involved, c. Gout
advantages of the commonly d. Hepatic coma
used methods of uric, area, e. Others
creatinine and ammonia 5. Other Kidney Function Tests
determination 7 hrs
7. Recognize the effect of age and Laboratory:
sex on the value of BUN, BUA, 1. Methods for NPN measurement
creatinine 2. Laboratory Safety
3. Laboratory Waste Management
At the end of this unit, the student is IX. Proteins Interactive Engaged Compassion MCQ Answer Keys Textbooks
able to: Lecture: 5 hrs Discussion Learning Accuracy
1. Correlate test results with 1. Biochemistry Honesty TFQ E-books
pathologic conditions a. Classification Inductive Actual Confidentiality Rubrics on Case Analysis
2. Given an electrophoresis b. Functions Method on performance Responsibility MTQ PPT
pattern, identify & correlate c. Metabolism Case of assays for Reliability Rubrics on Lab. Performance
abnormal result with pathologic 2. Specimen Considerations Analysis Proteins Laboratory Manual
findings 3. Measurement of Proteins
3. Compute for the AG ratio and a. Methods Case analysis
interpret test results with - Colorimetric
pathologic conditions - Electrophoresis
4. Enumerate different types of b. Electrophoretic patterns
amino acids c. Reference range
5. Explain the process of amino 4. Clinical Significance
acid synthesis and metabolism
6. Discuss the different types of Laboratory: 7 hrs
aminoacidopathies 1. Methods of Protein measurement
2. Laboratory safety
3. Laboratory Waste Management

At the end of this unit, the student is X. Lipid and Lipoproteins Interactive Engaged MCQ Answer Keys Textbooks
able to: Lecture: 5 hrs Discussion Learning
1. Describe terms associated with 1. Biochemistry TFQ E-books
lipids a. Classification Inductive Actual Rubrics on Case Analysis
2. Describe lipids according to - Lipids Method on performance MTQ PPT
 composition, classification, - Lipoproteins Case of assays for Rubrics on Lab. Performance
properties b. Functions Analysis Lipids and Laboratory Manual
3. Discuss the metabolism of lipids c. Metabolism Lipoproteins
in the body 2. Specimen Considerations
4. Rationalize the requirements 3. Measurement of Lipids and Case analysis
regarding patient preparations; Lipoproteins
specimen collection; transport a. Methods
processing and handling - Colorimetric
5. Discuss the principle involved, - Electrophoresis
advantages and disadvantages b. Electrophoretic patterns
of laboratory methods of lipid c. Reference Range
and lipoproteins 4. Clinical significance
6. Enumerate the reference value a. Hyperlipidemia
of each lipid measured b. Hyperlipoproteinemia
7. Correlate laboratory results with 5. Lipoprotein Phenotyping
patients lipid or lipoprotein
status Laboratory: 7 hrs
8. Relate the laboratory data in the 1. Methods for Lipid and Lipoprotein
assessment of risk or Coronary measurement
Heart Disease (CHD) 2. Laboratory Safety
9. Discuss the significance played 3. Laboratory Waste Management
by cardiac proteins and
enzymes in the diagnosis of
heart diseases
10. Perform correctly laboratory
methods of lipid determination
11. Demonstrate safely precautions
during performance of tests
 ALIGNMENT OF TEACHING LEARNING ACTIVITIES (TLA) WITH COURSE OUTCOMES (UOs)
NO TEACHING CO1 CO2 CO3 CO4 CO5 CO6
1. Demonstration
2. Didactic
3. Discovery teaching

NO LEARNING
1. Laboratory works

2. Interactive lecture
3. Library work

ALIGNMENT OF ASSESSMENT TASK (AT) WITH COURSE OUTCOMES (UOs)

NO ASSESSMENT TASK CO1 CO2 CO3 CO4 CO5 CO6
1 1. Quiz
2 2. Return Demonstration
3 3. Post-lab exercise
4 4. Assignment
5 5. Seatwork
6 6. Practical Exam
7 7. Summary Report
8 8. Reporting
COURSE REQUIREMENTS:
Lecture:
1. Regular Attendance
2. Quizzes / Classroom Activity Outputs
3. Seatwork
4. Homework
5. Major Examinations

Laboratory:
1. Regular Attendance
2. Pre-Lab Quizzes
3. Post-Lab Report Sheets
4. Laboratory Performance
5. Seatwork
6. Homework
6. Practical Exam
7. Major Examinations

Both Lecture and Laboratory:

Final Comprehensive Exam
GRADING SYSTEM
Lecture ( cut-off) Laboratory (cut-off)
Criteria Percentage weight Criteria Percentage weight
Major Exam % Major Exam %
Quizzes % Practical Exam %
Other Requirements: Other Requirements:
Seatwork Pre-Lab Quizzes
Homework % Reports (Experiments & Post Lab) %
Performance
Seatwork
Attendance % Attendance %
Total 100% Total 100%

Grading Scheme:

Prepared by: Faculty ( Please include the date)

Recommending Approval Dean ( idate)

Approved by: VP Academic Affairs

Control No: ___________________

 College of Medical Technology/Medical Laboratory Science

Learning Plan/Instructors Guide in Clinical Chemistry 2

Course Title: Clinical Chemistry 2

Course Description:

The course is a continuation of Clinical Chemistry 1 and deals with the quantitative measurement of biochemical substances found in body fluids essentially blood. This involves the knowledge and understanding of the basic
concepts and principles of their metabolism, laboratory analyses, and pathophysiology. Quality assurance and safety are given due emphasis.

Course Credit: 4 units (3 units lecture, 1 unit laboratory)

Contact Hours: 3 hours lecture, 3 hours laboratory per week (54 hours lecture ad 54 hours laboratory per semester)

Prerequisites: Clinical Chemistry 1

Placement: Third Year, 2nd Semester

Course Learning Outcomes :

At the end of this course, the student is able to:
1. Explain the different biochemical substances, their metabolism, actions, reference ranges and variables that may affect their analyses
2. Correlate test results with pathologic conditions
3. Apply concepts and principles of instrumentation in laboratory
4. Perform basic laboratory calculations
5. Practice quality assurance and laboratory safety
6. Perform correctly laboratory assays used to measure concentration of specific analytes
7. Solve common problems encountered in the clinical laboratory
8. Manifest professionalism
References: (Latest edition)
1. Anderson, Shauna and Susan Cockyane. Clinical Chemistry: Concepts and Applications. USA: Waveland Press Inc., 2007.
2. Arneson, W. and J. Brickell. Clinical Chemistry: A Laboratory Perspective. USA: F.A. Davis Co., 2007.
3. Ashwood E., D. Bruns and C. Burtis. Tietzs Fundamentals of Clinical Chemistry 6th ed. PhiladelphiaL W.B. Saunders Co., 2007.
4. Ashwood E., D. Bruns and C. Burtis. Tietzs Textbook of Clinical Chemistry and Molecular Diagnostics 4th ed. Philadelphia: W.B. Saunders Co., 2007.
5. Bishop, Michael L. et.al. Clinical Chemistry: Principles, Procedures, Correlations, 5th ed. Philadelphia: Lippincott Williams, Philadelphia, 2005.
6. Crook, Martin. Clinical Chemistry and Metabolic Medicine 7th ed. USA: Hodder Arnold Publication, 2006.
7. Kaplan, A., A. Pesce and S. Kazmierczak. Clinical Chemistry: Theory, Analysis, Correlation 4th ed. Edinburgh: Mosby, 2002.
8. Lewandrowsky, Kent. Clinical Chemistry: Laboratory Management & Clinical Correlations. USA: Lippincott Williams and Wilins 2002.
9. Marshall, William and Stephen Bangert. Clinical Chemistry 5th ed. Edinburgh: Mosby, 2004.
10. McClatchey, Kenneth. Clinical Laboratory Medicine 2nd ed. USA: Lippincott Williams and Wilkins, 2002.
11. Mcpherson, Richard A. and Matthew R. Pincus. Henrys Clinical Diagnosis and Management by Laboratory Methods 21st ed. Philadelphia: Elsevier Inc., 2007.
12. Sacher, Ronald and Richard McPherson. Widmanns Clinical Interpretation of Laboratory Tests 11th ed. Thailand: F.A. Davis, 2000.
13. Scott M., A. Gronowski and C. Eby. Tietzs Applied Laboratory Medicine 2nd ed. USA: Wiley-Liss, 2007.
14. Wu, Allan. Tietzs Clinical Guide to Laboratory Tests 4th ed. Philadelphia: W.B. Saunders, 2006.

Electronic References:
1. http://webpages.chhs.niu.edu/williams/AHP318/ClinicalChem.htm
2. http://www.dgrhoads.com/links.shtml
3. http://www.kmcsystems.com/invitro_clinical.asp#1
4. http://www.ualberta.ca~intd410/departments/spoc.html
5. http://www2.apsu.edu/~thompsonj/clin-chem-page1.htm
 Unit Learning Outcomes Teaching Learning Activities Resources
Values
(ULOs) Content T.A. Assessment Tasks
Deployed
Lecture Laboratory Assess-ment Tools
At the end of this unit, the student is I. Liver Function Tests Didactic Engaged Compassion MCQ Answer Keys Textbook
able to: Lecture: 8 hrs Method Learning Accuracy MTQ
1. Appreciate the functions of the 1. Anatomy and Physiology of the Liver Honesty TFQ E-Books
liver 2. Bilirubin Lecture Actual Responsibility
2. Apply the appropriate test for a. Metabolism performance Confidentialit PPT prepared by the techer
evaluation of the specific liver b. Types Interactive of assays y Case analysis Rubrics in Case Analysis
function c. Methodologies Group reliability Search Lectures
3. Acquire the necessary skills and d. Reference Range Discussion Case analysis Assignment Rubrics in Lab.
values in the performance of the e. Clinical Significance Performance And
different liver function tests 3. Methods for Evaluation of Liver Output Report-back
4. Perform tests to assess liver Function presentation sessions Photos of Performance of Tests
function a. Excretory Tests
5. Identify factors that may affect b. Metabolic Tests
test results c. Conjugation and
6. Correlate results with various d. Detoxification Test
physiologic and pathologic 4. Differential Diagnosis of Jaundice
conditions
7. Discuss the different principles Laboratory: 9 hrs
of tests 1. Bilirubin determination
8. Discuss the factors that may 2. Laboratory safety
affect test results 3. Laboratory waste management

At the end of this unit, the student is II. Enzymology Didactic Engaged Compassion MCQ Answer Keys
able to: Lecture: 10 Lecture Learning Accuracy MTQ Textbook
1. Classify the enzymes 1. Enzyme hrs Honesty TFQ
2. Explain the properties of a. Definition Interactive Actual Lab. Responsibility Rubrics on Case Analysis E-Books
enzymes b. Nomenclature Discussion performance Confidentialit Case analysis
3. Relate the different enzymes c. Classification of assays y PPT prepared by the techer
with corresponding substrates, - Oxidoreductases Inductive reliability Assignment
pH, temperature and products of - Transferases Approach of Assignment Search Lectures
enzymatic reactions - Hydrolases Teaching Report-back
4. State the clinical significance of - Lyases sessions And
the different enzymes - Isomerases
5. Describe the principles involved - Ligases Photos of Performance of Tests
in the methods of enzyme d. Organ specificity
determination e. Isoenzymes Textbook
6. Perform procedures to f. Enzyme Kinetics
determine enzymatic activities g. Units of Enzymatic Activity E-Books
7. Correlate results with various 2. Enzymes of Clinical Significance
physiologic and pathologic a. Reactions catalyzed PPT prepared by the techer
conditions b. Isoenzymes Case analysis
c. Methods of determination Search Lectures
d. Reference values Assignment
e. Clinical significance And
f. Analytic variables Report-back Answer Keys
- Cardiac enzymes sessions Photos of Performance of Tests
- Hepatic enzymes Rubrics on Case Analysis
- Pancreatic enzymes
- Prostatic enzymes Rubrics on Assigment
- Bone enzymes
- Muscle enzymes Rubricsonn Lab.
- RBC enzymes Performance
- Others Engaged
Learning
Laboratory: 9 hrs Prelim Exam is
1. Determination of activity of specific Actual Lab. given after 2
enzymes performance units (combined
2. Laboratory safety of assays OTQ
3. Laboratory waste management

At the end of this unit, the student is III. Fluids and Electrolytes Blended Actual Compassion MCQ Answer Keys
able to: Lecture: 9 hrs Method: performance Accuracy MTQ Textbook
1. Recognize the importance of the 1. Water Distribution of assays Honesty TFQ
electrolytes necessary in a. Body compartments Didactive and Responsibility E-Books
homeostasis b. Clinical significance Inductive Case analysis Confidentialit Case Rubrics on Case Analysis
2. Explain the methods of 2. Major Electrolytes Method y analysis PPT prepared by the techer
laboratory determination for a. Physiology Lecture- Output reliability Rubrics on Assignment
electrolytes b. Methods Discussion presentation Assignm Search Lectures
3. Perform procedures for the c. Reference values ent Rubrics on Report Back Session
laboratory determination of fluids d. Clinical significance And
and electrolytes - Sodium Report-
4. Discuss the factors that may - Potassium back Photos of Performance of Tests
affect test results - Calcium sessions
5. Correlate electrolyte results with - Magnesium
various metabolic and - Chloride
pathologic processes - Bicarbonate
- Inorganic Phosphate
- Others
Laboratory:
1. Determination of specific electrolytes 9 hrs
2. Laboratory safety
3. Laboratory waste management
At the end of this unit, the student is IV. Trace Elements 8 hrs Didactic Engaged Compassion MCQ Textbook
able to: Lecture: Lecture Learning Accuracy Answer Key
1. Recognize the physiologic 1. Physiology Honesty E-Books
importance of trace elements 2. Methods Actual Responsibility Case
2. Explain the methods of 3. Reference values performance Confidentialit analysis Rubrics on Case Analysis PPT prepared by the techer
laboratory determination for 4. Clinical significance Interactive of assays y
trace elements 9 hrs Group reliability Assignm And Report Back Session Search Lectures
3. Perform procedures for the Laboratory: discussion ents
laboratory determination for 1. Determination of trace elements Rubrics on Lab. Performance And
trace elements 2. Laboratory safety Report -
4. Discuss the factors that may 3. Laboratory waste management back Photos of Performance of Tests
affect test results sessions
5. Correlate results with various
metabolic and pathologic Mid.
processes Term
Exam is
given
after 2
units
(combin
ed OTQ
At the end of this unit, the student is V. Acid-Base Balance Didactic ( Actual Compassion MCQ Answer Keys Textbook
able to: Lecture: 10 Lecture) performance Accuracy MTQ
1. Describe the role and 1. Definitions hrs of assays Honesty TFT Rubrics on Case Analysis E-Books
importance of blood gases in the 2. Physiology of Acid-Base Balance Responsibility
maintenance of body fluid a. Buffer Systems Interactive Confidentialit Case And Report Back Session PPT prepared by the techer
equilibrium b. Organs involved on Acid-Base Group y analysis
2. Distinguish the different balance discussion reliability Rubrics on Lab. Performance Search Lectures
metabolic and respiratory 3. pH and pOH Assignm Rubrics
disturbances in the body 4. Henderson-Hasselbatch Equation ents And
3. Explain the methods of blood 5. Disturbances in Acid-Base Balance
gas determination a. Acidosis Report - Photos of Performance of Tests
4. Perform procedures for the b. Alkalosis back
laboratory determination of 6. Compensatory Mechanisms sessions
blood gases 7. Specimen Considerations
5. Discuss the factors that may 8. Laboratory Determinations
affect test results a. Methods
6. Correlate blood gas results with b. Reference Values
various physiologic and
pathologic processes Laboratory:
7. Report results in an acceptable 1. Determination of pH, pO2, pCO2, 9 hrs
format HCO3
2. Laboratory Safety
3. Laboratory Waste Management
At the end of this unit, the student is VI. Tumor Markers Didactic Engaged Compassion MCQ Answer Keys Textbook
able to: Lecture: 9 hrs (Lecture) Learning Accuracy
1. Define tumor/neoplasm, 1. Definitions Honesty Case Rubrics on Case Analysis E-Books
transformation, malignancy and 2. Common Tumor Markers Interactive Actual Responsibility analysis
metastasis 3. Laboratory Determinations Group performance Confidentialit And Report Back Session PPT prepared by the techer
2. Discuss the relationship 4. Methods discussion of assays y Assignm
between cancer & immunology 5. Reference Values reliability ents Rubrics on Lab. Performance Search Lectures
3. Explain the various tumor 6. Clinical Significance Rubrics
markers and their methods of Report - And
detection Laboratory: 9 hrs back Rubrics on Essay
4. Perform procedures for the 1. Determination of common tumor sessions Photos of Performance of Tests
laboratory determination of markers
certain tumor markers 2. Laboratory Safety Final
5. Discuss the factors that may 3. Laboratory Waste Management Exam is
affect test results given
6. Correlate tumor marker results after 2
with various physiologic and units
pathologic processes (combin
ed OTQ
and
Essay
 College of Medical Technology/Medical Laboratory Science
Learning Plan/Instructors Guide in Clinical Chemistry 3

Course Title: Clinical Chemistry 3

Course Description:

The course is a continuation of Clinical Chemistry 2. The course includes Endocrinology, Toxicology and Drug Testing. Endocrinology deals with the study of the products of a group of structurally and functionally specialized
glands and their action in maintaining the chemical integrity of cell environment. Toxicology involves the study of substances introduced exogenously into the body. It is appointed into the areas of detection of drugs of abuse, environmental
carcinogens, toxins or poisons and monitoring therapeutic levels. The course includes a focus into the establishment of drug testing centers including the legal and technical mandates required in the local setting. Quality assurance and
safety are given due emphasis.

Course Credit: 2 units lecture

Contact Hours: 2 hours lecture per week (36 hours lecture per semester)

Prerequisites: Clinical Chemistry 2

Placement: Fourth Year, 1st Semester

Course Learning Outcomes :

At the end of this course, the student is able to:
1. Explain the different biochemical substances, their metabolism, actions, reference ranges and variables that may affect their analyses
2. Correlate test results with pathologic conditions
3. Apply concepts and principles of instrumentation in the laboratory
4. Perform basic laboratory calculations
5. Practice quality assurance and laboratory safety
6. Perform correctly laboratory assays used to measure concentration of specific analytes
7. Solve common problems encountered in the clinical laboratory
8. Manifest professionalism
References: Latest edition
1. Anderson, Shauna and Susan Cockyane. Clinical Chemistry: Concepts and Applications. USA: Waveland Press Inc., 2007.
2. Arneson, W. and J. Brickell. Clinical Chemistry: A Laboratory Perspective. USA: F.A. Davis Co., 2007.
3. Ashwood E., D. Bruns and C. Burtis. Tietzs Fundamentals of Clinical Chemistry 6th ed. PhiladelphiaL W.B. Saunders Co., 2007.
4. Ashwood E., D. Bruns and C. Burtis. Tietzs Textbook of Clinical Chemistry and Molecular Diagnostics 4th ed. Philadelphia: W.B. Saunders Co., 2007.
5. Bertholf, Roger and Ruth Winecker. Chromatographic Methods in Clinical Chemistry and Toxicology. USA: Wiley, 2007.
6. Bishop, Michael L. et.al. Clinical Chemistry: Principles, Procedures, Correlations, 5th ed. Philadelphia: Lippincott Williams, Philadelphia, 2005.
7. Crook, Martin. Clinical Chemistry and Metabolic Medicine 7th ed. USA: Hodder Arnold Publication, 2006.
8. Gardner, David and Dolores Shoback. Greenspans Basic and Clinical Endocrinology 8th ed. McGraw-Hill Professional, 2007.
9. Hayes, Wallace A. Principles and Methods of Toxicology 5th ed. USA: Informa Healthcare, 2007.
10. Hempel, Georg. Handbook of Analytical Separations: Drug Monitoring and Clinical Chemistry (Vol. 5). Philadelphia: Elsevier Science, 2004.
11. Kaplan, A., A. Pesce and S. Kazmierczak. Clinical Chemistry: Theory, Analysis, Correlation 4th ed. Edinburgh: Mosby, 2002.
12. Klaassen, Curtis. Casarett and Doulls Toxicology: The Basic Science of Poisons 7th ed. USA: McGRaw-Hill Professional, 2007.
13. Kronenberg, Henry M. et. al. Williams Textbook of Endocrinology 11th ed. Philadelphia: W.B. Saunders, 2007.
14. Lewandrowski, Kent. Clinical Chemistry: Laboratory Management & Clinical Correlations. USA: Lippincott Williams and Wilins 2002.
15. Marshall, William and Stephen Bangert. Clinical Chemistry 5th ed. Edinburgh: Mosby, 2004.
16. McClatchey, Kenneth. Clinical Laboratory Medicine 2nd ed. USA: Lippincott Williams and Wilkins, 2002.
17. Mcpherson, Richard A. and Matthew R. Pincus. Henrys Clinical Diagnosis and Management by Laboratory Methods 21st ed. Philadelphia: Elsevier Inc., 2007.
18. Sacher, Ronald and Richard McPherson. Widmanns Clinical Interpretation of Laboratory Tests 11th ed. Thailand: F.A. Davis, 2000.
19. Scott M., A. Gronowski and C. Eby. Tietzs Applied Laboratory Medicine 2nd ed. USA: Wiley-Liss, 2007.
20. Wills, Simon. Drugs of Abuse 2nd ed. USA: Pharmaceutical Press, 205.
21. Wong, Raphael and Harley. Drugs of Abuse: Body Fluid Testing. USA: Humana Press, 2005.
22. Wu, Allan. Tietzs Clinical Guide to Laboratory Tests 4th ed. Philadelphia: W.B. Saunders, 2006.

Electronic References:
1. http://library.med.utah.edu/WebPath/TUTORIAL/DRUG/DRUG.html
2. http://userpages.umbc.edu/~farabaug/sokolo1.html
3. http://webpages.chhs.niu.edu/williams/AHP318/ClinicalChem.htm
4. http://www.kmcsystems.com/invitro_clinical.asp#1
5. http://www.ualberta.ca~intd410/departments/spoc.html
 Unit Learning Outcomes Teaching Learning Resources
Values Assessment Tasks
(ULOs) Content T.A. Activities
Deployed
Lecture Laboratory Assessment Tools
At the end of this unit, the student is I. Clinical Endocrinology Didactic NA Compassion MCQ Answer Keys
able to: Lecture: 12 Lecture Accuracy TFQ Textbooks
1. Discuss the anatomy and 1. Review of Endocrine Glands hrs and Honesty MTQ E-books
physiology of endocrine glands 2. Hormones Interactive Responsibility Rurbics on Case
2. Identify the corresponding a. Source Discussion Confidentiality Analysis PPT with photos
hormones produced by the b. Site of action Reliability
endocrine glands c. Principal action
3. Describe the characteristics of d. Specimen considerations Inductive Rubrics on
the different hormones produced e. Methods of determination Method on Assignement Photos or Schematic Presentations of
by various endocrine glands and f. Reference range Case analysis Laboratory Tests like T3, T4 and TSH
their function 3. Dysfunctions in the following endocrine Rubrics on Report
4. Explain the laboratory assays of glands: Microteaching Back sessions
hormones a. Hypothalamus on
5. Correlate given results of b. Pituitary gland Assignment First Prelims is
hormonal assays with various c. Thyroid gland given after
physiologic and pathologic d. Parathyroid gland Report-back discussion of 5
conditions e. Adrenals sessions endocrine
6. Discuss the different laboratory f. Pancreas glands
assays g. Gonads ( Ovaries and Testes)
7. Explicate the Laboratory Safety h. Pineal Gland (OTQ-
and Laboratory Waste i. Placenta Combined)
Management j. Gastrointestinal organs (
Stomach and Intestines ) Mid Term is
k. Kidneys given after
l. Heart discussion of all
Glands
Laboratory (optional): (OTQ-
1. Determination of hormones Combined)
2. Laboratory safety
3. Laboratory waste management
At the end of this unit, the student is II. Toxicology Didactic NA Compassion MCQ Answer Keys Textbooks
able to: Lecture: 15 Lecture Accuracy TFQ E-books
1. Explain the significance of 1. Definitions hrs ( Lecture Honesty MTQ Rubrics on Case
identifying toxic substances 2. Areas of Toxicology Interactive only) Responsibility Analysis PPT with photos
2. Describe the clinical signs and a. Substances/ Drugs of Abuse Group Confidentiality
symptoms of toxicity b. Therapeutic Drugs Discussion Reliability
3. Discuss preventive measures c. Environmental Carcinogens Rubrics on Assignment
and treatment of toxicity d. Toxins or Poisons Inductive Photos or Schematic Presentations of
4. Identify various toxic substances 3. Methods of Detection/Determination in Method on Rubrics on Report Laboratory Tests like Tests for Drugs
that can affect the body Serum and Urine Case analysis Back sessions
functions NA ( MCQ Answer Keys
Lecture TFQ PPT of Forensic Laboratory
only) MTQ Rubrics on Case
Analysis
 a. Immunochemical Methods Microteaching Rubrics on Assignment
5. Acquire the necessary skills - Enzyme-mediated (multiplied) on Report
required in the detection, immunologic technique Back Session Rubrics on Report
identification and qualification of - Fluorescence polarization on Back sessions
toxic substances immunoassay Assignment
6. Correlate given results of b. Chromatographic Procedures
toxicology assays with - Thin-layer Chromatography
physiologic and pathologic - High Performance Liquid
conditions Chromatography
7. Explicate the Laboratory Safety - Gas Chromatography with Mass
and Laboratory Waste Spectrometry
Management c. Other Laboratory Identification Didactic
Methods Method
4. Drugs of Abuse Lecture
a. Opiates
b. Tranquilizers: Benzodiazepines Interactive
c. Sedatives Hypnotics: Group
Barbiturates Discussion
d. Dopaminergic Pathway
Stimulants Inductive
e. Hallucinogens Case analysis
5. Therapeutic Drugs
a. Cardiotropics Report Back
b. Anticonvulsants Sessions on
c. Antiasthmatics Assignment
d. Anti-inflammatory
e. Immunosuppressives
f. Sedatives
g. Neuroleptics, antipsychotic
h. Major Tranquilizers
i. Other Chemotherapeutic Agents
6. Environmental Carcinogens Final
a. Benzopyrine Examination is
b. Nitrites given after
c. Aflatoxin Toxicology
d. Aromatic hydrocarbon OTQ (
7. Toxins and Acute Poisons Combined)
a. Classification of Poisons
b. Route of Administration
c. Action of Poisons
d. Conditions modifying the Action
of Poisons
e. Entrance and Elimination of
Poisons
f. Diagnosis of Poisoning
g. General Treatment of Poisoning
 Laboratory (optional):
1. Performance of tests
2. Laboratory safety
3. Laboratory waste management
At the end of this unit, the student is III. Drug Testing Interactive Actual Compassion MCQ Answer Keys
able to: Lecture: 9 hrs Group performanc Accuracy TFQ
1. Identify the drugs of abuse 1. Drugs of Abuse Discussion e of assays Honesty MTQ
tested in a clinical laboratory 2. Specimen Considerations Responsibility Rurbics on Case Analysis
setting 3. Methods of Detection Inductive on Case Confidentiality
2. Discuss the proper ways as well a. Screening Case analysis analysis Reliability
as precautionary measures b. Confirmatory Rubrics on Assignement
associated with the handling of 4. Drugs of Abuse Testing Laboratory Report Back Output
specimen for drug testing. session on presentatio Rubrics on Report Back
3. Acquire the necessary skills Laboratory: Assignment n sessions
require in the detection of drugs 1. Performance of tests
of abuse 2. Laboratory safety
4. Explain the legal and technical 3. Laboratory waste management
requirements needed in the
establishment of a drug testing
center.