The Effect of Polyphenol Robusta Coffee Beans (Coffea Robusta) in the Commented [dT1]: Diganti Polyphenol

Superoxide Dismutase (SOD) on Neutrofil Invitro

Tantin Ermawati*, Zahara Meilawaty*, Happy Harmono*, Dessy Rachmawaty
*Departement of Dental Biomedic, Dental Faculty of Jember University, Jember, Indonesia
Jl. Kalimantan no.37, Jember, Indonesia. e-mail ; tantin.ermawati@gmail.com Commented [dT2]: Tulisan penulisnya di perbesar sedikit ya
Commented [dT3]: Email tantin.ermawati@gmail.com

Abstract
Background. Neutrophils are the first body defense cells that act against an infectious and activate
NADPH oxidase to release large amounts of ROS. Polyphenols are active compounds in robusta coffee
beans (Coffea robusta). It has been widely known as antioxidants property that play role is superoxide
dismutase (SOD).

Objective of this research is to investigate the effect of polyphenol robusta coffee beans in vitro on SOD
production in neutrophil.

Methode

Peripheral venous blood

human

Neutrophils Isolate

Neutrophil + Neutrophil + Polyphenol Polyphenol Polyphenol Polyphenol

RPMI LPS extract 3,13% extract 6,25% extract 12,5% extract 25%
+ LPS + LPS + LPS + LPS

ELISA Test

SOD Level
Result

Table1. Mean Value of Superoxide Dismutase (SOD)

Groups Average SOD (U/ml) Commented [dT4]: Diganti (U/ml)

X SD
KP 1 206.1 5.57
KP 2 156.0 23.1
KP 3 201.8 12.9
KP 4 208.7 56.4
KP 5 222.6 21.7
KP 6 261.7 51.5
KP 1 :Neutrophils (negative control group)
KP 2 :Neutrophils exposed with LPS (positive control group)
KP 3 :Neutrophils+ 3.13% polyphenol extract + LPS
KP 4 :Neutrophils+ 6,25% polyphenol extract + LPS
KP 5 :Neutrophils+12,5% polyphenol extract + LPS
KP 6:Neutrophils+ 25% polyphenol extract + LPS Commented [dT5]: Diganti Neutrophils, polyphenol

Mean of SOD level

300

250

200

150

100

50

0
KP1 KP2 KP3 KP4 KP5 KP6

Figure 1. Diagram of the Mean Value of Superoxide Dismutase (SOD). Bars represent mean from a represented
experiment in triplicate (n = 3).

The obtained data were analyzed by using one way ANOVA and no significant difference were
found between the treatment groups. In Table 1, KP 6 showed the highest SOD production,
whereas KP 2 showed the lowest one. Based on the tables above, it is known that the SOD level
is increased in the treatment groups compared to the control group (KP2).

CONCLUSION
Polyphenol extract of Robusta coffee beans has wide biological activities for health in
general, and one of them is its properties as an antioxidant which is proved by the elevation of
SOD enzyme level where the highest level is observed in 25% polyphenol group.
ACKNOWLEDGEMENT

This study was supported by DRPM, Directorate General for Research and Development,
Ministry of Research, Technology and Higher Education 2016 from PDUPT Grant.