Академический Документы
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Культура Документы
1)International
Space Radiation Laboratory, National Institute of Radiological Sciences, 491,
Anaganwa, Inage-ku, Chiba-shi, Chiba 263-8555 and 2)Disease Model Cooperative
Research Association, 351, Aoihigashi, Hamamatsu, Shizuoka 433-8114, Japan
Table 1. Hematological values and concentration of total protein in WKY, SHR and SHRSP
Erythrocyte WKY 732 28 811 14* 784 61* 718 34 732 15 705 14
(104/ml) SHR 786 15# 898 17*, # 901 12*, # 746 26 822 99 780 45*,#
SHRSP 768 26# 876 57*,# 799 123 698 33 889 30*,# 761 122
Hg WKY 15.6 0.6 15.7 0.2 15.8 0.9 15.6 0.6 14.8 0.3* 14.5 0.3*
(g/dl) SHR 15.9 0.4 16.2 0.2# 16.0 0.3 15.6 0.5 15.6 1.2 14.9 0.8*
SHRSP 15.8 0.5 16.0 0.9 14.6 1.8 14.9 0.8 16.6 0.5*,# 14.1 2.3
Ht WKY 43.2 1.6 42.9 0.7 42.2 2.2 41.3 1.8 39.7 0.8 38.2 0.7*
(%) SHR 44.6 1.1 45.4 0.8*,# 43.8 0.8# 41.0 1.6 43.2 2.3# 40.9 2.0#
SHRSP 41.4 1.5# 43.6 2.3 41.5 5.6 36.7 1.6# 44.8 1.6*, # 39.2 6.6
Leucocyte WKY 115 25 88 10* 91 16 86 9 82 14 91 7
( 102/ml) SHR 97 17 90 11 92 10# 83 12 73 15* 79 12
SHRSP 68 16# 59 8*,# 80 19* 61 15# 48 7# 90 27*
Thrombocyte WKY 95 11 83 3* 82 4* 88 4 80 4* 68 5*
(104/ml) SHR 71 15# 70 4# 63 7# 64 24# 72 9# 65 6
SHRSP 76 6# 48 11*,# 77 20 61 27# 65 4# 77 20
* Significantly different from the value at the age of 8 weeks in each strain (p<0.05). # Significantly different from the value of
WKY at the same age (p<0.05). Values are mean SD.
70 S. FUKUDA, S. TSUCHIKURA AND H. IIDA
TP WKY 5.5 0.2 6.4 0.2* 6.4 0.3* 5.6 0.4 6.3 0.4* 6.3 0.2*
(g/dl) SHR 5.7 0.2# 6.3 0.2* 6.5 0.3* 5.5 0.2 6.0 0.1* 6.1 0.1*,#
SHRSP 5.6 0.3 6.1 0.2*,# 6.7 1.0* 5.2 0.2# 6.4 0.2* 6.4 0.8*
GOT WKY 48.7 4.8 53.8 15.6 69.8 9.4* 54.3 11.2 51.9 9.0 97.5 22.3*
(U/l) SHR 48.7 14.7 60.8 27.3 99.4 37.7* 43.7 2.3# 51.7 5.2* 47.8 9.6#
SHRSP 38.2 4.4# 49.6 15.8* 69.2 23.2* 33.8 1.6# 50.6 5.0* 63.8 17.8*,#
GPT WKY 20.6 1.1 19.5 5.5 21.7 3.5 19.1 2.7 18.7 2.3 31.6 8.6*
(U/l) SHR 29.5 10.7 30.1 9.0# 86.2 67.6*,# 20.0 0.8 21.6 4.8 26.1 3.9*
SHRSP 26.0 2.4# 29.5 21.1 45.7 33.4 22.4 1.6# 26.1 3.6*,# 28.7 9.8
ALP WKY 291 36 141 8* 152 12* 204 11 111 18* 157 13*
(U/l) SHR 252 20# 145 10* 135 19*,# 186 13# 101 16* 125 16*,#
SHRSP 229 23# 136 34* 135 34* 173 12# 120 11* 136 26*,#
BUN WKY 14.0 1.2 20.3 2.0* 17.2 1.6* 17.3 2.0 19.9 2.2* 19.7 2.3
(mg/dl) SHR 16.0 2.6# 24.7 1.4*,# 20.4 2.7*,# 12.1 0.8# 17.4 1.3* 14.7 0.9*,#
SHRSP 12.0 1.4# 16.4 2.6*,# 19.7 6.1*,# 11.8 1.7# 16.5 1.5*,# 18.5 2.9*,#
Creatinine WKY 0.419 0.019 0. 527 0.047* 0.576 0.053* 0.466 0.022 0.559 0.049* 0.585 0.02*
(mg/dl) SHR 0.409 0.039 0.465 0.041* 0. 590 0.039* 0.448 0.013 0.483 0.040*,# 0.566 0.052
SHRSP 0.426 0.039 0.469 0.052# 0.691 0.138*,# 0.453 0.045 0.547 0.056* 0.671 0.062*,#
Glucose WKY 171 9 156 10* 157 21 159 19 167 17 158 15
(mg/dl) SHR 165 14 159 8 191 14*,# 156 15 165 63 215 37*,#
SHRSP 177 16 164 17 183 31 175 18 186 14# 167 26
Total Ca WKY 10.0 0.4 9.5 0.3* 9.2 0.6* 10.3 0.3 10.0 0.5 9.6 0.3*
(mg/dl) SHR 9.8 0.6 9.5 0.4 9.0 0.4* 9.6 0.7# 9.6 0.7 9.4 0.6
SHRSP 10.3 0.5 10.3 0.3# 11.2 1.0*,# 10.0 0.4# 10.8 0.4*,# 10.5 0.8#
Phosphorus WKY 8.4 0.7 5.7 0.7* 5.6 0.8* 8.0 0.4 5.7 0.7* 6.0 1.1*
(mg/dl) SHR 8.0 0.7# 6.3 0.7* 5.9 1.1* 7.8 0.8 6.9 0.8# 6.0 0.9*
SHRSP 7.6 0.5# 5.9 0.7* 7.9 1.0# 7.1 0.6# 6.8 0.8# 6.7 1.3#
Ca+ WKY 1.28 0.04 1.24 0.03* 1.25 0.02 1.28 0.04 1.26 0.04 1.29 0.02
(U/l) SHR 1.26 0.04 1.23 0.03 1.22 0.03 1.23 0.05# 1.23 0.05 1.24 0.02#
SHRSP 1.35 0.05# 1.33 0.03# 1.42 0.06*,# 1.38 0.07# 1.32 0.03*,# 1.39 0.05#
Na+ WKY 174.3 4.8 141.5 1.5* 140.8 1.5* 169.8 3.5 141.5 1.1* 141.6 1.2*
(U/L) SHR 165.9 3.4# 143.5 1.4*,# 145.1 1.8*,# 163.7 4.2# 143.0 1.1*,# 143.5 1.5*,#
SHRSP 164.4 2.2# 142.5 1.7* 148.8 9.3*,# 162.2 4.0# 143.4 1.5*,# 143.1 1.5*
K+ WKY 5.40 0.82 4.71 0.28 4.92 0.79 5.15 0.94 4.40 0.38* 4.38 0.44
(U/L) SHR 4.88 0.26 4.56 0.37 4.40 1.18 4.77 0.98 4.86 1.22 3.98 0.37#
SHRSP 4.59 0.46# 4.31 0.42# 4.45 0.86 4.32 0.22# 4.81 1.27 3.91 0.31*
Cl WKY 97.4 1.4 99.7 0.9* 101.5 2.2* 98.1 1.1 100.3 1.6* 101.7 2.3*
(U/L) SHR 100.9 1.4# 101.3 1.3# 105.4 3.3*,# 101.6 1.5# 101.9 1.3 90.2 32.2
SHRSP 101.7 0.7# 106.2 9.7 101.6 3.6 101.7 1.3# 103.1 1.2# 100.7 4.6
* Significantly different from the value at the age of 8 weeks in each strain (p<0.05). # Significantly different from the value of WKY at
the same age (p<0.05). Values are mean SD.
all strains, but was lower in male and female SHR and at 8 weeks of age. The phosphorus decreased at 16 and
SHRSP at 8 weeks and in female SHRSP at 30 weeks. 30 weeks in all strains, except for male SHRSP at 30
The values of BUN and creatinine increased at 16 and weeks. The values of Ca2+ were higher in the SHRSP
30 weeks in all strains. The glucose values were high than in the WKY and SHR at all time points. The
at 30 weeks in male and female SHR. Total calcium values of Na + decreased at 16 and 30 weeks in all
was lower in the SHR, and higher in the SHRSP than strains, while K+ and Cl showed no particular ten-
the WKY at all time points, except for female SHRSP dency.
BLOOD AND BODY WEIGHT VALUES IN SHR, SHRSP, WKY 71
Table 3. Weights of various organs in WKY, SHR and SHRSP (values are percent as 104 of body weight at 8, 16 and 30 weeks of age)
Brain WKY 83.97 5.52 53.70 2.79* 44.13 7.05* 114.37 2.92 85.78 3.82* 67.75 11.14*
SHR 76.56 5.43# 56.18 2.10* 44.76 2.54* 110.67 4.68 89.91 5.67* 61.21 9.06*
SHRSP 83.84 5.25 63.67 3.99*,# 77.16 29.99# 114.88 3.73 87.12 2.06* 96.17 13.66*,#
Heart WKY 37.45 3.52 28.59 1.67* 28.77 3.45* 36.22 2.72 30.37 1.19* 31.91 2.86*
SHR 46.63 6.24# 40.30 1.92*,# 35.72 3.94*,# 50.90 4.83# 48.29 2.40# 42.41 4.47*,#
SHRSP 49.55 4.87# 45.87 3.19# 55.83 5.09*,# 51.26 4.74# 45.22 3.40# 61.59 8.35*,#
Lung WKY 43.02 1.63 32.37 5.55* 32.09 2.22* 47.91 2.12 43.46 3.93 40.53 3.74*
SHR 47.05 2.07# 41.95 6.26# 37.33 6.49 54.84 5.97# 58.05 12.89# 39.51 6.67*
SHRSP 47.78 6.54 46.24 4.93 64.65 27.47# 49.60 4.98 52.37 14.93 63.05 13.40
Thymus WKY 17.57 0.84 7.03 0.54* 6.15 0.88* 21.62 2.90 9.65 1.02* 10.43 3.44*
SHR 13.12 1.87# 4.76 1.02*,# 5.37 1.09* 15.83 3.47# 6.39 2.12*,# 7.46 2.42*
SHRSP 15.71 1.34# 4.94 1.10* 5.56 1.95* 20.46 1.69 8.10 1.06*,# 8.28 3.64*
Liver WKY 429.53 24.56 348.41 19.60* 283.58 20.23* 463.67 19.52 370.92 13.25* 358.98 21.58*
SHR 470.79 16.60# 436.35 32.14*,# 332.39 22.84*,# 476.21 22.88 385.44 18.54*,# 336.91 25.37*,#
SHRSP 446.06 33.25 416.70 16.88*,# 420.18 44.69# 427.75 32.22# 380.54 15.43* 443.40 43.05#
Kidneys WKY 41.18 2.13 32.23 2.12* 29.81 2.30* 40.80 1.88 33.68 2.67* 31.18 1.92*
SHR 44.21 2.22# 37.20 2.07*,# 32.63 1.54*,# 44.49 3.37# 37.11 2.26*,# 33.87 2.93*,#
SHRSP 44.84 1.62# 39.09 2.13*,# 44.59 4.29# 49.16 3.28# 37.19 3.84*,# 43.36 4.16*,#
Adrenal glands WKY 1.30 0.21 0.63 0.12* 0.50 0.16* 1.67 0.26 1.37 0.22* 1.34 0.48*
SHR 0.96 0.14# 0.69 0.14* 0.48 0.11* 1.81 0.37 1.26 0.22* 0.92 0.21*,#
SHRSP 1.16 0.17 0.72 0.14*,# 0.76 0.20*,# 1.46 0.23# 1.25 0.13* 1.46 0.45
Spleen WKY 17.92 0.73 12.55 0.55* 10.63 0.63* 18.06 0.61 14.07 0.79* 13.54 1.88*
SHR 15.71 0.86 14.03 0.77*,# 10.78 0.73* 16.58 1.90 15.91 2.04# 13.21 1.05*
SHRSP 16.87 1.40# 16.50 1.62# 21.04 5.12# 17.60 1.31 16.95 1.03# 29.18 9.74*
Pancreas WKY 26.44 6.10 22.83 3.96 24.90 4.94 35.72 4.93 34.17 6.68 32.36 12.44
SHR 23.56 4.53 22.70 5.38 23.89 5.45 31.95 4.73 38.06 8.74 32.67 14.33
SHRSP 23.38 4.89 26.59 10.23 29.01 13.11 30.60 4.79 31.17 11.44 46.06 15.31*,#
Testes /Ovaries WKY 73.01 2.83 54.36 3.21* 27.37 9.13* 2.17 0.55 1. 80 0.30* 1.41 0.43*
SHR 69.92 5.76 51.65 3.99*,# 31.90 2.78* 1.99 0.41 1.59 0.44*,# 1.18 0.19*,#
SHRSP 67.11 10.98 67.71 5.56# 41.71 4.78*,# 1.74 0.43# 1.39 0.33* 1.43 0.49*
Digestive tract WKY 495.00 45.96 361.40 26.18* 215.61 50.22* 580.49 42.66 405.27 19.75* 363.68 31.92*
SHR 459.41 41.00 352.84 32.77* 226.34 21.94* 546.95 19.11 402.81 51.09* 302.10 19.32*,#
SHRSP 425.35 25.69# 325.46 18.48*,# 328.91 25.95*,# 507.64 31.17# 418.78 19.94*,# 468.79 43.37*,#
Muscle WKY 3.17 0.30 2.88 0.16 3.05 0.27 3.24 0.22 3.27 0.11 3.33 0.46
(Soleus) SHR 4.00 0.12# 4.12 0.65# 4.11 0.30# 4.57 0.49# 4.13 0.44# 4.07 0.19#
SHRSP 3.33 0.24 2.86 0.26 3.25 0.44 3.19 0.19 2.64 0.45*,# 3.71 0.43*
* Significantly different from the value at the age of 8 weeks in each strain (p<0.05). # Significantly different from the value of WKY at
the same age (p<0.05). Values are mean SD.
The values for the organ weights are shown in Table SHRSP than the WKY and SHR at 30 weeks of age.
3, in which they are presented as the rate ( 104) of At autopsy, brain hemorrhage in the SHRSP was ob-
body weights to compare strains and sexes, because the served in 8 of 11 males and 10 of 12 females, and
large strain and sex differences in body weights were thereafter irregularities such as hollow portions and/or
seen at 16 and 30 weeks of age. Also, the weights of unevenness on the surface was found in all rats. Val-
bilateral adrenal glands, kidneys, and testes/ovaries were ues for the heart decreased with aging in all strains
combined because there were no significant differences except in male and female SHRSP at 30 weeks of age,
between right and left sides. The brain weights de- and at each of the measured points, those of the SHRSP
creased with aging, and were lower in males than in were significantly higher than in the other groups, fol-
females in each strain, and significantly higher in the lowed by the SHR. The values for the lung and pancreas
72 S. FUKUDA, S. TSUCHIKURA AND H. IIDA
in the SHRSP were higher at 30 weeks old than those the small intestine [24]. The values of various items
at 8 and 16 weeks, and also than the SHR and WKY at in the SHRSP at 30 weeks of age varied with large
30 weeks. Thymus values decreased with aging in all individual differences, suggesting that the alterations in
strains. Values for the liver, kidneys, adrenal glands, various organs, including the brain, might be progress-
spleen, and digestive tract decreased in all strains with ing due to prolonged hypertension. Although the
aging, and also were higher in the SHRSP than the present study was carried out to obtain fundamental
WKY and SHR at 30 weeks. Values for the testes and data for SHR, SHRSP, and WKY rats, further studies
ovaries decreased in all strains with aging. Values for such as those involving pathological examinations will
the soleus were higher in the male and female SHR at be necessary to clarify the features of these strains.
all measured points.
Regarding the clinical features of the SHRSP strain Acknowledgments
which has blood pressure elevating with aging, the hair
becomes bristly and dirty, and the temperament of the We would like to thank Mr. Xueming Yan for his
animals becomes nervous in response to stimulation help with the autopsy of rats and statistical analysis of
such as sound and restraint compared with SHR and the data.
WKY. These findings may be related to the increase in
weight of the adrenal glands. Nevertheless the body References
weight of the SHRSP continued to increase with aging,
with the increases in the values for various organs such 1. Fukuda, S., Tsuchikura, S., Iida, H., Ikeda, K., Nara, Y.,
and Yamori, Y. 1992. Genetic Hypertension. 218: 421
as the brain, heart, lung, liver, and spleen, suggesting 423.
the presence of alterations in these organs due to con- 2. Fukuda, S., Iida, H., Tsuchikura, S., Nara, Y., Ikeda, K.,
tinuous hypertension over a long period. However, the and Yamori, Y. 1994. Jpn. Heart J. 35: 532.
almost complete lack of change in the levels of serum 3. Fukuda, S., Iida, H., Tsuchikura, S., Ikeda, K., Nara, Y.,
and Yamori, Y.1995. Jpn. Heart J. 36: 508.
biochemical constituents such as GOT, GPT, and ALP
4. Fukuda, S., Tsuchikura, S., Iida, H., Ikeda, K., Nara, Y.,
indicates that the physiological functions of these or- and Yamori Y.1995. Clin. Exp. Pharmacol. Physiol. 22:
gans may stay relatively normal. However, the increases S240241.
in weight of the kidneys with elevations of BUN and 5. Tsuchikura, S., Fukuda, S., Iida, H., Ikeda, K, Nara, Y., and
Yamori, Y. 1992. Genetic Hypertemsion. 218: 417419.
creatinine may indicate the presence of functional dam-
6. Yamori, Y., Fukuda, S., Tsuchikura, S., Ikeda, K., Nara,
age and alterations. The high values of total and ionized Y., and Horie, R.1991. Clin. And Exper. Hyper.-Theory
calcium might be related to not only hypertension, but and Proctice. A13: 755762.
also osteoporosis, probably due to a release of calcium 7. Yamori, Y. 1984. Experomental and genetic models of
by parathyroid hormone against hypocalcemia caused hypertension. pp. 240255. In: deJong W. et. Handbook of
Hypertension. Elsevier, Amsteram.
by urinary high Ca excretion and low Ca absorption of