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Centrifugal Microfluidics

Basic Concept of Centrifugal Microfluidics

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Why Using a Centrifugal System?
flow rates 1 nL/s 100 mL/s
great range for microfluidics
pumping insensitive to pH, ionic strength, chemical
composition
life science and chemical applications available
fluid gating by passive structures
cost efficient
easy to implement
easy combination of fluidic elements for diverse fluidic
operations
low cost fabrication technology
miniaturization feasible

Centrifugal Force

centrifugal force for all fluid elements at radius r is constant


high parallelization
pulse-free transport of liquids
sedimentation processes due to different densities
fluid transport only in radial direction

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Centrifugal Fluid Transport

Centrifugal Fluid Transport


fluid propulsion driven by:
centrifugally induced pressure
rotational frequency
geometry of channels
channel and reservoir location
fluid properties

centrifugal fluid transport is insensitiv to


physiochemical properties of fluids
ionic strength
pH
conductivity
various analytes

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Forces and Effects on a
Centrifugal Platform

Capillary Action

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Capillary Fluid Transport

Hydrophobic Valving and Flow Control

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Hydrophobic Valving and Flow Control

Geometric Valving

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Siphon Valving

Capillary Fluid Transport

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Hydrophobic Metering

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Splitting/Aliquoting

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Splitting/Aliquoting

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Shake-mode Mixing

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Coriolis Mixing
single channel mixer
mixing depends on:
rotational frequency
downstream position of liquid in channel

Ducree, Ultrafast
Micromixing by Coriolis-
Induced Multi-Lamination
of Centrifugal Flow, 2004

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Coriolis Switching

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A Gyrolab CD microlaboratory consists of 96 or 112
individual microstructures, which are processed in parallel,
under the control of Gyrolab Workstation. Each
microstructure contains a column pre-packed with
streptavidin-coated beads that are activated with
biotinylated capture reagents as the first step in the
immunoassay.

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